eChapters

The udder is responsible for milk production. It is composed of four mammary glands each with its own teat (Fig. 167). Swelling of udder or teats is an indication of udder disease warranting clinical exploration and palpation. Visual examination the udder (before and after milking) and palpation is of great significance in detecting abnormalities. The udder is visually inspected from back and sides for symmetry and also palpated thoroughly. Palpation is a simple, quick and easy routine diagnostic tool for mastitis detection. Teats and supra mammary lymph nodes should also be palpated. These examinations aid in detecting fibrosis, inflammation or atrophy. Diffuse fibrosis gives the quarter a firm feeling. Areas of local fibrosis may vary in size and shape. Hot, painful and diffuse swelling with marked abnormality of mammary secretion is seen in acute mammary inflammation (acute mastitis). Atrophy of the gland is the terminal stage of chronic mastitis. With the development of gangrene, the area becomes cold and bluish. Five point palpation scores (Petrovski et al., 2011; Hogan et al., 1995; Scaletti and Harmon, 2012)

All across the world, the dairy industry is facing numerous problems and changes. Dairy development has received a considerable boost from factors including access to technical advancements, improvements in infrastructural facilities, and the development of modern marketing insights. It is interesting to see how the dairy sector is changing globally.

Dairy farmers are faced with a number of fundamental inquiries. The first inquires as to what dairy animal he needs to keep. Is the breed with the best production performance the one that has adapted to the local environment the most? What animal is the best, asks the second? Is it the one with a showwinning conformation or the one with a strong ancestry? Is the ideal dairy cow one that produces the most milk, has the best feet, legs, and udder support, or combines the performance of these characteristics in the best possible way?

In terms of their contribution to human nutrition, the dairy industry is very important. Since dairy farming has the ability to offer meaningful selfemployment and stable income, it is a dependable source of subsistence for the rural poor. Additionally, it requires less labour and cash to maintain. Dairy cattle feeding directly affect the animals’ ability to reproduce and produce. Therefore, the addition of a mineral mixture is necessary to maximize the animal’s productivity and milk output.

Introduction

Use of acid/base is common in laboratories for experiments like titration and proper safety in their handling laboratory is necessary. In this chapter we will briefly discuss about the safety measures necessary to be adopted while using acids/bases in laboratory for any experiment.

Handling of Strong Acid/Base

There are four major points that need to be taken care of while working with strong acids or bases that might be quite harmful if any accident happens.

1. Protect Yourself

Wear a lab-coat and acid-resistant gloves: Always use lab-coat/apron and make it sure that the sleeves properly cover your arms. More importantly, use gloves made of acid-resistant materials and also wear shoes to protect your feet while wearing long-pants/jeans. Female with long hair, must tie her hair properly (with rubber band) to avoid any disturbance caused by appearance in between.

Wear safety goggles: It is important to wear safety goggles to protect your eyes using larger and fitting goggles that cover front as well as sides of your eyes. Always use properly fitting and adjustable goggles because smaller/ loose goggles may not protect the areas around your eyes against accidental acid spills and it may fall down from your face, if it is too loose.

Locate emergency wash area: There must be an emergency washing area nearby the work place or laboratory containing lab spill management kits. All the workers in the laboratory must be aware of using the kits and emergency plans.

Neutralize acid: Don’t panic even if an acid or chemical spill happens. Stay calm and act as quickly as possible. In case an acid spills, neutralize it by using sodium bicarbonate so that they can be cleaned and disposed safely. If acid comes in contact of skin, wash it off under running tap water but do not use/ apply sodium bicarbonate on skin.

The cell is the unit of structure of all plants and animals. With increasing complexity of organization, masses of different cell types tend to become localized and to form recognizable patterns. An aggregation of cells forming a definite pattern is called tissue. A still higher level of organization, tissues aggregate to form organs. The animal cell is a highly organized structure. Plant cell is different from animal cell  by having a cellulose cell wall.  There are a number of well defined structures viz., nucleus, mitochondria, lysosomes, Golgi apparatus etc in the cell. Animal cells also have centrioles whereas plant cells contain plastids such as chloroplasts. The remainder of the cell is the cytoplasm.

Care and treatment of animals is possible by the combined efforts of all the stake holders of animals shared by the owners, consumers and veterinarians. Although, veterinary services are available throughout the country, involving different stakeholders including veterinarian, there is an entrenched lack of empathy and a profound disconnect among stakeholders when it comes to identifying with the pain and discomfort of animals. 
Veterinarians participate in two ways in animal welfare and animal ethics. One is the role of the veterinarian as an individual member of a profession. The other is the collective role of the profession, as expressed by the professional bodies.

Types of cells : Animal cells are usually defined by the tissue of their origin. On the basis of morphology, they can be divided into 5 types.

    1.    Epithelial cells : Epithelial tissues consist of a layer of cells which cover organs and line cavity. Example skin and the lining of alimentary canal. They grow well as single cell monolayer in culture.

    2.    Fibroblast cells : Connective tissue form the major structural component of animals. The tissue contains fibroblasts and most widely used cell in the laboratory. Fibroblasts are bound to the fibrous protein collagen in the connective tissue. They have excellent growth characteristics.

A finite growth capacity is a characteristic of all cells derived form the normal animal tissue. The cells pass through a series of age related changes before being incapable to divide further. The finite number of generations of growth is a characteristic of the cell type, age and species of origin and referred to as the “Hayflick limit”. The cells derived from embryonic tissue have a greater growth capacity than those derived from adult tissue.

Animal Welfare Board of India was started under the stewardship of Late Smt. Rukmini Devi Arundale, well known humanitarian. The Animal Welfare Board of India is a statutory advisory body on Animal Welfare Laws and promotes animal welfare in the country. The board established in 1962 under Section 4 to 10 of the Prevention of Cruelty to Animals Act, 1960 (No. 59 of 1960).  The primary sources of funding of the AWBI are grants from the Government of India. However, contributions, subscriptions, bequests and gifts will also be encouraged.

Domestication is the most extensive biological experiment ever undertaken by man. It has involved millions of animals and has extended through many centuries. It still continues and animal domestication was one of the major contributory factors to the agricultural revolution during the Neolithic period, which resulted in a shift in human lifestyle from hunting to farming. There are two species of domestic duck, the common duck, domesticated from the wild mallard (Anas platyrhynchos), and the Muscovy duck (Cairina moschata). Both are members of the Anatinae. In both species, the same scientific binomial is used for the domestic and the wild forms. Inter-breeding between the two species occurs, but their offspring are infertile. Both species of domestic duck produce eggs and meat and both are valuable on farms and smallholdings for reducing pests.

Once cells are obtained from a culture collection or isolated from animal tissue,, cells are put in the flask with growth medium. The cells are inoculated at a density of 104-105 cells/ml to get a concentration of 106 cells/ml or 105 cells/cm2 in a solid surface in 2-3 days because of nutrient limitation, accumulation of toxic metabolite or lacking of growth surface.

The process of civilization and materialist life style has forced man to make laws which govern or rule the conduct of the people of society or community. In the earlier days the relation of human and animal was interdependent and mutual. Now a days man has become self-centered and ignorant of his surroundings. This has led to cruelty on the animals in many circumstances. Therefore, in pursuit of prevention of the cruelty to animals following laws, regulations have made by the government.

Cell growth and cell passage : Different types of cells have different growth requirements but in all cases the surrounding medium must supply all the essential nutrients. The temperature, pH, osmolarity and humidity must be kept within limits. Again, toxic or inhibitory substances must not be allowed to accumulate. Serum, a source of macromolecular growth factors, is essential to the growth of many cell types. Anchorage dependent cells require nontoxic or biologically inert glass or plastic surface for attachment.

A laboratory is essential for research work in science and development. Most of the research institutes have dedicated laboratories for researchers and students to conduct experiments, perform research work, as well as to provide hands-on learning/training to students. There are certain rules and regulations laid down by the laboratory in-charge which need to be followed strictly for safety purposes. Some of the laboratories keep ‘safety manual’ for researchers to read before entering into the laboratory to conduct experiments. Whenever safety is compromised in a laboratory, there is increase in risk of unwanted incidents in the laboratory. Storing food/beverages in the same refrigerator where laboratory chemicals are kept may lead to disastrous effects on health. This chapter aims to provide instructions in brief on the things to be performed (Do’s) before and after entering onto a laboratory as well as the things never to be done (Don’ts) in a laboratory for the safety reasons.

Good Laboratory Practices (Do’s)

• P Wear apron/labcoat while working in the laboratory.
• P Use gloves/respiratory mask/fumehood while handling toxic/volatile/inflammable chemicals/reagents.
• P Use pippet or pippetor for safety in transfer of solutions.
• P Protect eyes, face and naked parts of the body from UV radiations. Use safety goggles or face mask/apron.
• P Keep your eyes away from Liquid Nitrogen and use cryogloves while handling it.
• P Arrange all the necessary materials/solutions before starting the experiment.
• P Periodically fumigate/ozonize culture and inoculation rooms.
• P Autoclave all contaminated materials before discarding.
• P Pre-treat gel or solution containing Ethidium Bromide (EtBr) before discarding into laboratory waste-disposal bin, and use gloves while handling them.
• P Burn all the transgenic plant materials in an incinerator after taking necessary observations.
• P Make entry in the designated log-book and clean the instrument after using it properly.
• P Switch-off the instrument after use or when not in use.
• P Wash hands with soap and remove the labcoat before leaving/going out of the laboratory7.
• P Ensure that switches for fans/lights, and water taps are off while leaving the laboratory.
• P Follow the biosafety guidelines suggested for handling and disposal of the hazardous chemical.

Vero (monkey, African green, kidney) 
Morphology: Fibroblast
Species:, African green monkey 
Tissue: Kidney

Properties: Virology, virus titration, virus replication, plaque assays, bacterial cytotoxicity

2.1     Regulation of the Cell Cycle 
    
2.2     Mitosis
    
2.3     Meiosis

Introduction
Antimicrobial resistance (AMR) represents one of the most significant public health challenges of modern times. The discovery of antibiotics in the early 20th century revolutionized medicine, transforming once-fatal infections into manageable conditions and enabling life-saving procedures such as organ transplantation, chemotherapy, and joint replacement surgery. However, the overuse and misuse of antibiotics in human medicine, veterinary practice, and agriculture have accelerated the development of resistant microorganisms. Today, infections caused by resistant pathogens claim millions of lives worldwide each year, with the World Health Organization warning of a postantibiotic era in which minor injuries or routine surgeries could again become life-threatening. Although AMR is often framed within the context of hospital-acquired infections and clinical treatment failure, its implications extend much further. In particular, AMR has significant consequences for physical and health fitness. For athletes, soldiers, patients in rehabilitation, and physically active individuals, resistant infections can disrupt performance, delay recovery, and compromise long-term health. The increasing prevalence of resistant organisms in community environments—including gyms, swimming pools, and sports facilities—further raises concern. This chapter situates AMR at the intersection of clinical medicine, sports science, and community health. It explores how resistant pathogens affect physically active populations, complicate rehabilitation processes, and influence outcomes in both competitive and recreational Fitnes. It also highlights the global and national responses to AMR, preventive strategies relevant to Fitnes and rehabilitation, and the role of healthcare professionals

Sheep and goat rearing in India continues to be traditional even today. This sector is primarily in the hands of poor, landless or small and marginal farmers who raise their animals on natural vegetation, stubbles and supplement tree lopping. The productivity of sheep in India is relatively low with an average body weight gain of 20 to 50 g/day. The major causes for the low productivity are inadequate grazing resources, tropical heat and disease incidences and a lack of awareness about improved management practices.

Small volume cultures are useful for experiments such as studying cell morphology, titration of viruses, plaque assay, pock assay, SNT and to compare the effect of agents on growth and metabolism. However, there are other application where there is a need of large number of cells such as extraction of DNA/RNA from cells, production of viruses for vaccine production and a number of products namely interferons, interleukins, hormones, enzymes, antibodies, glycoproteins etc. The large quantity of cells can be obtained by using a large number of flasks/bottles but it is tedius, labour intensive, expensive and there is a risk of contiamination. Although the unit processes are more cost effective and efficient, it needs some modification to overcome limiting factors such as oxygen depletion, shear damage and metabolic toxicity. There are a number of aberrations available to scale up cell culture described below.