eChapters

Introduction

In developing countries, ruminants thrive mainly on poor quality feeds and pastures, whose nutritive value is poor. They are having a low content of nitrogen, energy, minerals and vitamins and high content of lignin and silica, which limit their efficient utilization by ruminants. Therefore, these poor quality feeds and fodder needs to be upgraded in terms of their nutritive value using various treatments like physical, chemical, physico chemical and biological methods to improve intake and utilization of nutrients from poor quality roughages. However, supplementation of critical nutrients like energy, protein, minerals, vitamins also improved the productivity and health of the ruminants (Dass et al., 2011). Availability of oil cakes as protein supplement in developing countries is very poor and at a very high price, This has led to use of non-protein nitrogen source as urea, to compensate for the nitrogen deficiency of fibrous feeds (Dutta et al., 2009). Thus, enhancing intake, digestibility and availability of nutrients from poor quality roughages are only possible through optimization of rumen fermentation. The use of solid multi-nutrient block supplementation /urea-molasses blocks/ multi nutrients blocks/ pashu chocolates to provide optimum levels of various nutrients primarily for better growth of rumen micro organisms. They need rich sources of fermentable nitrogen, minerals and vitamins, which are lacking in the fibrous feeds like cereal straws (Sahoo et al, 2009). This offer several advantages such as easy transport, storage and handling, and reduced risks of poisoning as compared with other approaches, such as giving a small amount of urea in drinking water, sprinkling of urea solution on fibrous feeds before feeding, or urea-ammonization of crop residues (Dutta et al ., 2009).

Introduction

Incidence of ectoparasitism is on rise in the livestock population, impeding its production and performance. Those areas which were free of ectoparasites are now under great parasite load because of changing climatic conditions and global warming. Global warming has resulted in vectors shifting their habitats to temperate regions also. Ectoparasites are not only causing irritation to livestock by blood sucking and associated pruritus, they are serving as vectors for a variety of haemoprotozoan and rickettsiae leading to far-flung complications. Major ectoparasites infesting ruminants are ticks, lice, fleas and flies. Though ticks and flies are proclaimed as notorious for transmission of haemoprotozoans, now a days evidences are on rise reporting the role of fleas and lice as vectors for various haemoprotozoan and viral diseases. The need of the hour is to explore more and more ectoparasites to identify their vector potential and their effective control measures. While addressing the ectoparasitism in livestock, another major problem arises with their control. Most ectoparasitism is of seasonal nature at least in temperate areas, while it is a round the year phenomenon in tropics. Only very few compounds are available as ectoparasiticides and used by clinicians, and has resulted in onset of resistance among ectoparasites. New compounds need to be developed and marketed to counter this and at the same time it should be environment friendly also. Lest their rising use can lead to eradication of friendly fauna which can be more detrimental to the ecosystem. Now a days researches are focusing on the development of integrated tick/vector control measures which are making use of minimal chemical compounds and utilizing the synergistic application of farming techniques/practices and cultivating plants of acaricidal properties. In this era of pandemic, zoonotic nature of various haemoprotozoan/rickettsiae affecting livestock is also of concern to the mankind. So, they need to be addressed at the earliest considering their impact. The susceptibility to ectoparasitism varies from animal to animal.

1    Consent form for surgery

2    Consent form for euthanesia

3    Slaughter certificate

4    Wound certificate

5    Proforma for export certificate of dogs

Heat stress induced by climate change is a serious issue of livestock industries worldwide (Bajagai, 2011). When environmental temperatures move out of the thermo-neutral zone (or comfort zone) animals begins to experience either heat stress or cold stress. Both these stresses require the animals to increase the amount of energy used to maintain the body temperature so that less energy is available for productive processes. Atmospheric temperature, relative humidity, air movement and solar radiation are the major factors that determine the heat load on animal. Thermoregulation is the ability of the animals to maintain their body temperature in cold or hot environments. It consists of behavioural, physiological and anatomical responses that affect energy metabolism. At lower temperature as a physiological adjustment to maintain the body temperature the animal increases the metabolic heat production by increasing the basal metabolic rate and uncoupling of oxidative phosphorylation. Hence the maintenance energy requirement of animals increases in a cold environment, which reduces the amount of energy available for production. From a practical point of view higher temperatures are much more dangerous for producing animals than a cold environment. Temperatures exceeding the higher critical level compromise animal performance not only by changing the energy and nutrient metabolism, but also by upsetting the body homeostasis, with detrimental consequences both for immune competence and for product quality. In general, livestock with high production potential are at greatest risk of heat stress, and require most attention.

Climate change is one of the most serious and long standing challenges facing livestock around the world and has moved up to the top of agenda among scientific community, civil society, business and government. Majority of scientific experts around the world believe that the climate change is already occurring by human activities, by use of fossil fuel, deforestation and agricultural practices, livestock rearing and that the developing countries in particular would be more vulnerable to the continuously changing climate. Rise in temperature due to climate change is likely to affect livestock production and health. Large deltas and low lying coastal areas would be inundated by a rise in sea level along with increased precipitation intensity. (DFID, 2004).Major contributors to the climate change are the man-made greenhouse gases, such as carbon dioxide, methane and nitrous oxide and hydroflurocarbons in the atmosphere. The accumulation of gas is causing the climatic change globally, as evident from the increased frequency of floods, droughts, cyclones and torrential rains in the recent past. The green house gases (GHGs) emission from agriculture sector alone accounts for 25.5% of global emission and over 60% from anthropogenic sources (FAO,2009)

1. Introduction
Animal experimentation remains a cornerstone of scientific research in many fields, from medical advances to pharmaceutical testing. However, the ethical treatment of laboratory animals has become a point of significant concern worldwide. India, through the Committee for the Control and Supervision of Experiments on Animals (CCSEA), ensures that ethical standards are maintained in research institutions. Established under the Ministry of Environment, Forests, and Climate Change (MoEF&CC), CCSEA is tasked with overseeing the use of animals in experiments, ensuring that procedures adhere to ethical guidelines and international norms. CCSEA’s guidelines are in line with international standards set forth by organizations such as the International Council for Laboratory Animal Science (ICLAS) and the European Union (EU), but they are tailored to address local needs and conditions. The guidelines, which focus on the welfare of laboratory animals, emphasize the ethical review process, animal care, and the importance of minimizing animal suffering. They also stress the need for researchers to implement the 3Rs—Replacement, Reduction, and Refinement—to minimize the use of animals and ensure humane practices in research. This chapter explores the CCSEA guidelines in detail and compares them with animal welfare regulations from around the world, including the United States, European Union, Japan, and Australia. By understanding the similarities and differences between these regulations, researchers and policymakers can ensure the ethical treatment of laboratory animals on a global scale.
2. Objectives of CCSEA Guidelines
The main objective of the CCSEA guidelines is to ensure that laboratory animals are treated ethically and humanely while also supporting the validity and scientific rigor of research. The guidelines encourage researchers to prioritize animal welfare while minimizing unnecessary suffering. Specifically, CCSEA focuses on:
1. Ethical Treatment of Animals: Ensuring that animals are housed in suitable conditions, given adequate veterinary care, and provided with a comfortable environment to reduce stress.
2. Justification of Animal Use: Ensuring that animals are used only when necessary, and that their use is scientifically justified.
3. Pain and Stress Minimization: Ensuring that experimental procedures cause the least pain, distress and suffering possible.

Introduction
The use of animals in scientific research has been a cornerstone of medical advancements for decades. However, this practice has also been the subject of intense ethical debate. This lecture explores the necessity of animal experimentation, the ethical considerations involved, and the regulatory frameworks in place to ensure the humane treatment of laboratory animals.
The Need for Laboratory Animals
Animal experimentation has played a crucial role in numerous medical breakthroughs. The importance of laboratory animals in research cannot be overemphasized. Among the various purposes for which humans use animals, experimentation involves relatively few animals and serves a noble purpose in advancing scientific knowledge and human health.
Laboratory animals are invaluable in the drug development process for several reasons:
• Similarity to human organ systems, metabolism, and enzyme functions.
• Suitability as alternatives to human subjects.
• Small size and ability to be reared in controlled environments.
• High reproductive rates.
• Potential for genetic purity, leading to reproducible and reliable results.
The Importance of Animal Welfare
While the benefits of animal experimentation are clear, it’s equally important to consider the ethical implications of using animals for research. The concept of animal welfare is embedded in the Indian Constitution, which states that it is the duty of every citizen “to protect and improve the natural environment including forests, rivers, lakes and wildlife, and to have right compassion for the living creatures” (Article 51 A (g)). This ethical consideration for animals has been emphasized by philosophers, spiritual leaders, and scientists throughout history:

The skin is the largest organ of the body that plays an important role in defending the body against minor physical injuries, providing some defense against microbial invasion, maintenance of homeostasis, water conservation, temperature conservation, supporting hair growth and heat loss. Changes in the secretion of sebaceous glands lead to compromised defence. Abnormalities of the skin may arise from specific skin disease or by the poor general health status. Therefore, a detailed clinical examination of the animal as a whole and of its skin in particular, is an essential part of the process of diagnosis. The skin has three main layers starting from outside as the epidermis, the dermis and the subcutis. The epidermis consists largely of epithelial cells and pigment. The dermis is a connective tissue layer containing blood vessels, nerves, hair follicles, sebaceous and sweat glands. The inner most subcutis contains fibrous and fatty tissues that provides insulation and support. In healthy animals, the skin has considerable elasticity. It reduces in dehydration and inflammation / injury to skin. General clinical examination should be carried out first as a routine followed by special examination

One Health is defined as “the collaborative effort of multiple disciplines working locally, nationally, and globally to attain optimal health for people, animals and the environment.” The concept of ‘One Health’ dates back to the Greek physician Hippocrates (ca. 460 BCE–a.370) who in his text “On Airs, Waters and Places” stated:

Whoever wishes to investigate medicine properly, should proceed thus: in the first place to consider the seasons of the year, and what effects each of them produces, for they are not all alike, but differ much from themselves in regard to their changes.

Large Animals The collection and evaluation of cerebrospinal fluid (CSF) in farm animals with signs of diseases of nervous system provides very valuable diagnostic and prognostic information. Routinely, CSF is evaluated for total protein level, erythrocyte and leukocyte counts, and a differential cell count. Additional tests are available for specific neurologic conditions. CSF is primarily produced by the choroid plexuses in the lateral, third, and fourth ventricles through plasma ultra filtration and active transport across the blood-brain barrier. Smaller amounts of CSF are generated by the ependymal lining, pia arachnoid, meningeal blood vessels, and spinal cord’s central canal. The turnover rate is about 1% per minute, meaning systemic electrolyte or acid-base changes take several minutes to affect CSF

Introduction
Good Laboratory Practice (GLP) has been a cornerstone of laboratory operations, ensuring quality, reliability, and reproducibility in research and development across various fields, including pharmaceuticals, biotechnology, and environmental science. As we move further into the 21st century, the landscape of GLP is evolving rapidly, influenced by technological advancements, regulatory changes, and the increasing complexity of scientific research. This article explores future trends and innovations in GLP, delving into key areas such as automation, data management, regulatory shifts, and sustainable practices.
1. The Role of Automation in GLP
1.1 Increased Efficiency and Accuracy
One of the most significant trends in GLP is the integration of automation. Laboratory automation includes the prompt use of technology to perform tasks in a much efficient way with minimal human interventions, thus significantly increasing efficiency and accuracy of laboratory practices. Automated systems can handle repetitive tasks such as liquid handling, sample preparation, and data analysis, reducing the potential for human error.
Benefits of Automation
• Reduction of Human Error: Automation minimizes the chances of mistakes that can occur when humans perform these repetitive tasks, thereby improving the reliability of the results.
• Speed up the Research Process: For example, robotic systems can be used in drug discovery. These systems can conduct high-throughput screening, which means they can quickly test thousands of different compounds for potential effectiveness as drugs.

India has an enormous wealth of cattle, which is crucial to the development of the socioeconomic status of millions of rural households as well as the country’s economy. In addition to giving millions of people access to lowcost nutritious food, this industry plays a significant role in boosting family incomes and creating gainful employment in rural areas, notably among landless laborers, small and marginal farmers, and women.

Introduction

While experimenting with chemicals, sometimes we need to dry the chemical/ solution (solid, liquid or gas) using a process like evaporation or distillation, mainly to remove water or different solvents. Drying is the process of evaporation or distillation to remove water/solvent from a solution, solid or even from gas. Generally, a drying agent (usually an anhydrous inorganic salt) is used for this process, which reacts with the water (present in the chemical/ solvent to be dried) to form a hydrate. For example, anhydrous MgSO4 is one of the drying agents. The drying agents are recognized by their capacity (the rate at which they absorb water) and their intensity (the amount of water left behind in the solvent at equilibrium). No chemical reaction or change in phase occurs during the drying process.

Type of Solvents

Apart from water (a universal solvent), there are three families of organic solvents used in laboratories.

1) Oxygenated solvents: Alcohols, Ketones, Glycol ethers, Glycol esters, etc.

2) Hydrocarbon solvents: Aliphatic and Aromatic hydrocarbons.

3) Halogenated solvents: Chlorinated hydrocarbons.

Dairy farmers hope to have better milking cows than those in their current herds. A solid foundation is necessary for every farming operation. Undoubtedly, the dairy farm documentation system is among the most important facets of farm management. Tracking each cow’s productivity within a herd and assisting breed development generally are the major objectives of a dairy farm tracking system. This information could be referred to as production data.

Introduction

For agricultural applications as well as for research work, often we need to prepare solutions of different agrochemicals of varying strength, as per the requirements. Agrochemicals are used to help crop plants grow, produce, and protect them from biotic/abiotic stresses. Many of the agrochemicals (insecticides, herbicides, fungicides, rodenticides, molluscicides, nematicides, etc.) are used to protect the crop from insect pests and diseases. However, some of them (fertilizers and soil conditioners) are used to augment crop yield. While in the previous chapter (Chapter 8) we discussed about preparation of laboratory solutions, in this chapter we will briefly discuss about the preparation of different agrochemicals.

Agrochemical/agrichemical is a generalized term used for the chemicals applied in agriculture. In general, it also includes the feed-supplements used in f isheries and animal husbandry.

Agrochemicals

Fertilizers

Fertilizers are used to augment growth of crop plants and to mitigate nutrient deficiency in the soil. Fertilisers can be organic (containing natural substances, prepared through natural processes) and inorganic fertilisers (synthetic fertilizers, manufactured artificially using chemical processes using natural deposits, by chemical alterations).

Soil Conditioners

Soil conditioners include manure, livestock and leaf compost, peat that help making the soil in good condition. They are laid on the top soil and then mixed properly. They not only increase nutrient contents in the soil but also improve aeration and water holding capacity.

AMR has been prevalent in almost all European countries from past decades. A 2021-2022 report published by the European Food Safety Authority (EFSA) and the European Centre for Disease Prevention and Control (ECDC) on AMR in zoonotic and indicator bacteria from humans, animals and food origin reported vast differences in antimicrobials resistance levels among the European countries, with comparatively higher AMR percentages in the southern and eastern parts of Europe (ECDC, 2022, 2023). The resistance levels ranged from very high for tetracyclines, sulfonamides and ampicillin, moderate for ciprofloxacin to low levels for third-generation cephalosporins (cefotaxime and ceftazidime) in human cases infected with Salmonella spp. in 2022 (ECDC, 2023). The trends are dynamic and over a decade of 2013 2022, the human isolates from 12 and 15 countries reported a statistically significant reduction in tetracycline and ampicillin resistance, particularly for S. typhimurium (ECDC, 2023). The ciprofloxacin and cefotaxime combination, a highest priority critically important antimicrobials (CIA), had very low and rare resistance levels for Salmonella isolates of humans and animal and their derived meat.

Livestock species wise variation was also observed in the resistance pattern in imported meat, being higher in poultry meat isolates compared to pig or cattle meat for Escherichia coli (ECDC, 2023). High to extremely high levels of f luoroquinolone resistance from Campylobacter jejuni and C. coli of both human and animal origin isolates exist, due to which the fluoroquinolones can no more be used as a treatment option for human Campylobacter infections (ECDC 2023). The combined occurrence of erythromycin resistance and ciprofloxacin in Campylobacter spp is considered of high public health importance, and the report mentioned that the combined resistance for these antimicrobials was higher in C. coli compared to C. jejuni from food-producing animals and humans. However, while the ciprofloxacin resistance showed an increase in over 13 study countries, seven countries reported reduced erythromycin resistance for C. jejuni. Overall, resistance to conventional antimicrobials was frequent in Campylobacter and Salmonella from humans and animals. Increasing trend in resistance to one of two CIA for treatment in humans has been observed in poultry-originated Salmonella serovars and Campylobacter while combined resistance was is low in few Salmonella serovars and C. coli isolates from humans and animals in some countries (ECDC, 2023).

The Indian economy, which meets its own dairy demands, has reached a turning point with its bovine breeding initiatives. The viability of this enterprise, however, depends largely on completing as many successful artificial insemination cycles at a cattle farm. Estrus behaviour observation is used to determine when artificial insemination should take place. But since not all cattle exhibit substantial estrus symptoms, insemination results are not always reliable. An estrus detecting instrument known as an Electronic Nose (eNose) is created to address these issues.

The term “Poultry” indicates to all the domesticated species of birds like chicken, ducks, turkeys, Japanese quail, guinea fowls, geese, pigeons, ostrich, emu etc. Ducks account for 7% of poultry population and are mostly found in coastal states of the country and in states with more lakes and rivers like West Bengal, Orissa, Andhra Pradesh, Tamil Nadu, Kerala, Assam, Jammu and Kashmir and Tripura. Duck farming is still in primitive stage and indigenous ducks outnumbered the exotic ducks in spite of their inferior performance.

In India 90-95% of ducks are indigenous or nondescript types, which are hardly, with mediocre egg production and highly suitable for extensive system of rearing.

Primary cells are often preferred to established cell lines as they better represent the cells in vivo in terms of activity and functions. On the other hand, there is significant difference between original culture and present culture and even between cultures of the same cells in different laboratories. The rate of growth increases and there is change in chromosome numbers of lines over the time. The differences in phenotypes can cause problems in comparison and interpretation of results. The advantage of cell lines are ease handling, grow continuously, large yield and readily available. Primary cell cultures compared to established cell line are more fastidious in their growth requirements because many cell lines are derived from tumour tissue and demonstrate reduced growth factor requirements.

Introduction 
Antimicrobial resistance (AMR) is one of the most significant global health challenges of the 21st century. Defined as the ability of microorganisms— including bacteria, viruses, fungi, and parasites—to resist the effects of antimicrobial drugs, AMR undermines the progress of modern medicine. Antimicrobials—including antibiotics, antivirals, antifungals, and antiparasitics—are the cornerstone of modern medicine. Their discovery transformed the treatment of infectious diseases and enabled surgical advances, chemotherapy, and organ transplantation. However, Alexander Fleming, the discoverer of penicillin, warned that misuse could render these drugs ineffective. Antibiotics, since their discovery in the 20th century, revolutionized healthcare, saving millions of lives and enabling complex medical procedures. Yet, the misuse and overuse of antimicrobials in human health, veterinary practices, and agriculture have rapidly accelerated the emergence of resistant organisms. Today, this warning has materialized as a global crisis. Antimicrobial resistance (AMR) is now considered one of the top ten public health threats by the World Health Organization (WHO). It is not only a biomedical issue but also a socio-economic and developmental challenge. This chapter aims to provide an in-depth overview of AMR, focusing on definitions, history, mechanisms, causes, global status, impacts, and the urgency of mitigation efforts. It also explores national and global responses and future directions to curb the spread of AMR. AMR is estimated to cause 700,000 deaths annually worldwide, with projections of 10 million deaths annually by 2050 if urgent interventions are not implemented. In addition to health impacts, AMR poses grave threats to food security, sustainable agriculture, economic stability, and global development. This chapter provides a comprehensive overview of AMR, including definitions and conceptual frameworks, historical background, mechanisms of