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Our animal companions are our family members. We want the best for them and want them to be healthy and happy. However, just as it is with everyone else, our companion animals can also fall ill and suffer from emotions such as grief, fear, and anxiety. And while we follow all expert medical advice, sometimes we may wish for something more – something that will make life easier for them as well as us. This is where the concept of energy healing for animals comes in – to comfort and support our animal companions. With joy, we watch them relax with a sigh; we see our nervous dogs calm down, and those recovering from an acute illness get back on their paws in no time. Energy healing for animals involves a holistic approach to ensure the physical, emotional, and spiritual well-being of our animal friends. It is based on the fundamental concept that just like us, our animal companions too have a subtle energy layer, and they too have vital life-force energies or Prana or Qi. And just like us, they, too, have chakras and meridians through which the Qi flows. Energy healing is a set of powerful techniques practiced to calm and restore the balance of the body. This system is largely based on the removal of energetic blocks and helps restore the body’s energy levels. It is crucial for those living with companion animals to understand the importance of energy healing for their animal companions, as it encompasses a broad range of alternative and complementary therapies designed to support the natural healing process of animals. Here are some proven, popular, non-invasive energy healing techniques that bring relief and comfort to companion animals.
1. Animal Reiki: The word Reiki means “mystical energy.” The system of Reiki for healing was revealed by Japanese Buddhist practitioner Mikao Usui in the early 1900s. And while it was initially meant for humans, practitioners soon discovered that animals responded much more swiftly and effectively to this gentle, non-invasive energy work.
2. EFT Tapping: This involves tapping on specific points on the body to free up emotional blocks. EFT Tapping for animals brings powerful transformations when an animal is struggling with emotional distress, such as grief over the loss of a loved human or animal.
3. Scalar Wave Healing: Scalar Wave was discovered in the 1900s by James Maxwell and later Nicolas Tesla. Scalar Wave harnesses the healing power of high vibrational frequencies meeting to create a standing wave, and practitioners use this to bring about healing at a cellular level.
4. Shamanic Healing: In this ancient technique, practitioners work with the elements of nature, such as plants, trees, and stones, as well as beings in other dimensions, to bring about healing. Shamanic healing can reach beyond space and time and has been very effective in releasing old trauma or emotions that are often hidden beneath layers of survival mechanisms.
5. Healing Mantra: Ayurveda, the Indian system of medicine, advocates about different types of Chikitsa. Daivavyapashraya Chikitsa is one of the treatment approaches concerned with Spiritual way of treatment. It includes so many approaches, amongst all Mantra Chikitsa is described in Ayurveda at various places as a potent approach. It is discussed for both the healthy and unhealthy conditions. Mantra comes from Sanskrit word which means sacred message or text, spell. The word Mantra is derived from Man+Tra, man—mananaat---just by chanting and tra—trayate---- we can protect ourselves. Means, just by chanting one can save himself in the universe. i.e. it is saved from onset of disturbances. Mantras are the words loaded with power and sounds capable of penetrating our body into deep levels. Mantra chikitsa is the ancient science originated from Vedas, this is a parallel science to Ayurveda also called alternative medicine system.Mantra Chikitsa is one among the Daivavyapashraya Chikitsa highlights the importance of concept of Mantra. In Atharva Veda and Kaushikasutra, the two most ancient and authentic sources of Daivavyapashraya. Mantra Chikitsa is mainly used for both preventive and curative aspect. It is also used for the enhancement of the Gunas of Aushadhi. Effect of Mantra is described as Prabhavajanya action. Prabhava means the specific and characteristic action of substances, it is called as Acintya. Mantra Chikitsa cure the Karmaja Vyadhi and Agantuja Vyadhi. In the Vedic period, Daivavyapashraya Chikitsa was followed in various rituals.

A useful method for quickly preparing tissue slides for microscopic analysis is the frozen section technique. There are numerous clinical and research contexts where the frozen section technique is employed. For quick intraoperative diagnosis in histopathology, frozen sections are frequently utilized, giving our surgical colleagues direction. To ascertain the extent of excision required to remove a skin tumour, the surgeon doing Mohs Micrographic Surgery depends solely on the frozen sections. The frozen section technique is used in many scientific applications to create microscopic slides using a variety of complex morphologic, immunohistochemical, and molecular techniques. There are several uses for frozen sections, including rapid diagnosis, immunohistochemistry, enzyme histochemistry, lipid detection, cancer margin research, and more. Making frozen section slides is a difficult technical procedure that calls for the mastery of advanced technical abilities in addition to knowledge of the pathology, microanatomy, and histology of the tissues under investigation. The outcomes will depend on our capacity to produce a high-quality preparation, whether it is utilized for research or intraoperative consulting. Dr. Louis B. Wilson’s 1905 description served as the basis for the frozen section technique used in medical labs today. At the request of surgeon Dr. William Mayo, one of the founders of the Mayo Clinic, Wilson devised the procedure based on previous studies. A frozen section was also used in earlier reports by Dr. Thomas S. Cullen of Johns Hopkins Hospital in Baltimore, but only after formalin fixation. Dr. William Welch, a pathologist at Hopkins, also tried Cullen’s technique, but there were no negative clinical effects. Wilson is therefore widely acknowledged as having really invented the procedure.

Introduction
Antimicrobial resistance (AMR) is increasingly recognised as a “silent pandemic,” threatening global health and development. In 2019 alone, bacterial AMR was directly responsible for 1.27 million deaths and contributed to nearly 5 million more worldwide. Projections warn that by 2050, unchecked AMR could result in 10 million annual deaths and impose a staggering economic burden of USD 100 trillion. A major driver of this crisis is the indiscriminate and often prophylactic use of antibiotics in livestock production systems, which account for a majority of global antimicrobial consumption. The One Health framework—emphasising the interconnectedness of animal, human, and environmental health—offers a strategic pathway for mitigating AMR. It requires dismantling sectoral silos and fostering collaboration among veterinarians, physicians, public health authorities, food safety regulators, and environmental scientists. Against this backdrop, NDDB has positioned itself as a frontrunner in India by embedding One Health principles into large-scale field programmes. Since 2014, NDDB has progressively promoted alternative medicine for bovine mastitis control under its Mastitis Conrol Project (MCP), subsequently expanding in 2017–18 into a broader Disease Control through Alternate Methods (DCAM) project with Ethnoveterinary Medicine (EVM) as the central pillar.
Building Up: From DCAM to DISHA as One Health Strategy
Building on the successes of MCP, DCAM, and BCP, NDDB launched the Dairy Integrated Safety and Health Action (DISHA) project for 2025–2030, DISHA consolidates NDDB’s One Health strategy by combining animal health management, zoonotic disease surveillance, and food safety monitoring. Its objectives include: managing bovine cases with EVM, organising farmer awareness programmes, training veterinarians and health workers, screening cattle as well as their owner for brucellosis, and testing bulk milk samples for aflatoxins and antibiotic residues. Importantly, DISHA also addresses emerging food system contaminants such as microplastics, per- and polyfluoroalkyl substances (PFAs), Bisphenol-A (BPA) and heavy metals, thereby broadening the scope of One Health beyond infectious diseases.

Floriculture has become very important industry as a result of science based techniques and steady supply of improved plant materials. One of the major constraints of floriculture industry is non availability of large scale quality planting materials. Conventional methods of propagation can not meet the increasing demand of propagating materials. Micropropagation is perhaps the most widely used biotechnology tool for large scale propagation of floricultural crops. This economic proposition has led to intensive practical research to evolve tissue culture techniques for mass propagation and production of virus-free plant material.  Since most of the ornamental crops are highly heterozygous, their seed progeny is not true-to-type. Conventionally in these plants vegetative reproduction method is followed to produce genetically identical plants at commercial scale. But the main constrain of this method is that only a limited number of plants can be raised in a year from a single mother plant and often fail to meet the market demand. Therefore, most of the modern floriculturists follow the micropropagation technique for rapid multiplication of ornamental plants.

World trade of fresh cut flower is increasing day by day. Fresh flowers lose their freshness and quality both during travel and also during and after arrangements due to flower specific short vase life. Such deficiencies can be ameliorated through application of nutrient additives to vase water. Use of preservative solutions has been known for many years to increase the vase life of cut flowers. Different methods like use of holding, pulsing and bud opening solution, growth regulators, gamma irradiation, pre-cooling, cold storage, packaging etc. are already in practice in the flower trade to ensure the garden fresh quality of flowers to consumers (Singh et al., 2001). The floral business is expanding in a big way, nationwide.  Sales of cut flowers are rising faster through retailers as every state has entered into development of supermarket chains. Cut flower sale through retailers is  a booming business. Retail growers and Wholesale growers are the major players in our country for domestic market. Retail market suffers from logistic bottlenecks. Retailers do not have any background scientific knowledge to increase the vase life of cut flowers. Flower buyers are very choosy and prefer quality bloom with longer vase life.

There are different crop specific societies in different countries for registering new varieties. This does not provide any intellectual property right. It serves as a documental proof of new variety, author, mode of origin, place and date. For example, there is The American Rose Society which serves as the International Cultivar Registration Authority for Roses; The Royal Horticultural Society, International Registration Authority for the genus Dahlia; Indian Agricultural Research Institute, New Delhi -110 012, India for Bougainvillea. Each society either follows the rules set forth by the International Code of Nomenclature for Cultivated Plants or they are having their own rules and regulations for registration (Anonymous 1981, Choudhury 1973, Singh 1983, 1986, 1987, 1999, Singh et al., 1999, Sindhu 2015, Singh 2007). Newly evolved Bougainvilleas are being registered by the Division of Vegetable Crops and Floriculture, Indian Agricultural Research Institute, New Delhi, since 1966. A check list of all the existing important cultivars based on the available information from literature as well as from amateurs, professional traders and other sources from different countries are available.

14.1 NEED FOR ANIMAL DISEASE INFORMATION SYSTEMS

Once the animal disease data are gathered, a few things need to be done with the data so collected. First, they must be managed and quality checked; Secondly, they must be analyzed to be understandable; and thirdly, they must be acted upon. For data to be analyzable and useful for decision-making, they need cautious management and quality control. An information system is the collection of data, people, procedures, hardware, software, files, and data required to accomplish an organized set of functions.

The constituents of an information system are:

• People – those who gather data, those responsible for data input and those responsible for data analysis

• A storage / retrieval / analysis system – which consists of computer hardware and appropriate software

• A feedback delivery system – mechanisms to ensure that processed data are fed back to data gatherers

Opportunities

India is home to largest milch animal population, totalling around 100 million cattle and buffalo (out of total population of 300 million) spread over 6.5 million census villages. India is the largest milk producer with CAGR of 4.5%, likely to go up to 5%, which is likely to create around 20 million jobs every year. The most significant factor is that dairy industry is likely to exceed IT industry in employment generation. In year 2018, India produced 146 million tonnes of milk contributing 26% of the agriculture GDP.

But the foundation of dairy industry is economically vulnerable as per animal productivity and fertility are very low leading to meagre profits and frequent demands to enhance procurement price. National schemes like Operation Flood, National Dairy Plan-I and National Dairy Plan II, largely focussed on milk collection, processing, distribution and organising farmers but the animal component has been neglected. Enhancing per animal productivity by empowering farmers to scientific management and breeding to achieve genetic improvement needs urgent attention. Although numerous technologies to improve health and genetics are available but the single most important gap is failure to deliver these to farmers. Unlike western world, India has not been able to develop modern veterinary service delivery models. In order to cater to breeding and health services of cattle and buffalo (300 million in numbers) and also pet animals in rural areas and small towns, huge manpower and infrastructure will be required.

Lectins are sugar-binding proteins or glycoprotein of non-immune origin. In 1954 William C. Boyd alone and then together with Elizabeth Shapleigh introduced term ‘lectin’ from the Latin word lego- ‘chosen’ (from the verb legere ‘to choose’ or ‘pick out’).The earliest description of a lectin is believed to have been given by Peter Hermann Stillmark in his doctoral thesis presented in 1888 to the University of Dorpat. Stillmark isolated ricin, an extremely toxic hemagglutinin, from seeds of the castor plant (Ricinus communis).
History
Lectins, although first discovered more than 100 years ago in plants, and defined by their ability to selectively recognize specific carbohydrate structures, are ubiquitous in living organisms and throughout nature. Long before a deeper understanding of their numerous biological functions, the plant lectins, also known as phytohemagglutinins, were noted for their particularly high specificity for foreign HYPERLINK https://en.wikipedia.org/wiki/Glycoconjugate glycoconjugates (e.g. those of fungi, invertebrates, and animals) and used in biomedicine for blood cell testing and in biochemistry for fractionation.
Hundreds of lectins of plant and animal origin have been described, and the list continues to increase. The first lectin to be purified on a large scale and available on a commercial basis was concanavalin A, which is now the mostused lectin for characterization and purification of sugar-containing molecules and cellular structures. The legume lectins are probably the most well-studied lectins.

Scanning electron-microscopes are available nowadays which operate at high vacuum as well as low vacuum mode. Low vacuum mode allows viewing of wet samples, but compromise on very high resolution and high magnification images. In high vacuum mode the samples must be absolutely dry and free of any organic contaminants to avoid gas molecules interfering with both the primary electron beam and the secondary or backscattered electrons emitted from the sample. Biological specimens which are largely composed of water, necessitate additional preparatory steps to ensure that structure of the organism is retained in its natural form.
Sample preparation is essential in scanning electron microscopy. Improper sample preparations can undermine the quality of results and lead to false conclusions. SEM, unlike TEM, allows the processing of larger sized organisms and tissues. For Biological samples the smallest representative sample size should be used as the microscope’s detection capacity is as much as 1μm from the sample surface.
Samples for SEM preparations are normally collected by biopsy/ dissection/ sacrificing of an animal to remove tissues from the main body. Incisions should be done with fresh sharp blades to avoid tissue deformation from the physical forces applied with blunt blades. Collected samples should be immediately transferred to vial containing the fixative for fixation.
Microbial cultures fixation of bacteria and fungus needs to be carried out under strict safety measures. Biosafety cabinets should be used wherever possible and sample should be removed from the biosafety cabinets only after the

Being polytocous animal, sow give birth several (8-14) piglets in each farrowing, mother pig find several deficiencies and disorders during gestation, farrowing and postnatal conditions. Prior to birth intrauterine competition amongst the feotus piglets for nutrition and gas exchange is a big stress for abortion. Several infectious and non-infectious causes may hamper proper growth and pathological conditions resulting mortality (15-20%) in piglets than weaner and adults (Sarma, et al, 2012). Several risk factors are also responsible for piglet mortality.

Prenatal factors: Insults during pregnancy can impact a piglet’s ability to survive after birth and regulate their body temperature. Some maternal aspects that affect survability and mortality amongst piglets are prolong farrowing duration more than 4 hours, number of parity of sow, usually first and second parity show high mortality than subsequent parities and also older sow lose more piglets.

Introduction

Climate change, a global phenomenon of present time, has emerged as the greatest challenge of the world in the twenty-first century. Rising earth’s surface temperature is one of the most threatening factors coming into view. The substantial changes in the earth’s climate have an impact on ecosystems, economics and societies in a variety of ways (Sharma et al., 2024). It is one of the important environmental, agricultural, human and animal health issue. Climate change is an association of multidimensional effects involving physical characteristics, causes and consequences (Visschers, 2018). The climate change has resulted in to increasing global average temperature of air and ocean, melting of snow, rising of ocean level, change in the rainfall pattern and extreme whether events such as drought, flood, heat waves, storms etc.

Nanotechnology is manipulation of matter on an atomic, molecular, and supramolecular scale. The earliest, widespread description of nanotechnology referred to the particular technological goal of precisely manipulating atoms and molecules for fabrication of macroscale products, also now referred to as molecular nanotechnology.
Nanotechnology as the manipulation of matter with at least one dimension sized from 1 to 100 nanometers. This definition reflects the fact that quantum mechanical effects are important at this quantum- realm scale, and so the definition shifted from a particular technological goal to a research category inclusive of all types of research and technologies that deal with the special properties of matter which occur below the given size threshold. It is therefore common to see the plural form "nanotechnologies" as well as "nanoscale technologies" to refer to the broad range of research and applications whose common trait is size.
Introduction
A nanometer (nm) is one thousand millionth of a meter. A single human hair is about 80,000 nm wide; a red blood cell is approximately 7,000 nm wide, a DNA molecule 2 to 2.5 nm, and a water molecule almost 0.3 nm. The term ”nanotechnology” was created by Norio Taniguchi of Tokyo University in 1974 to describe the precision manufacture of materials with nanometer tolerances, but its origins date back to Richard Feynman’s 1959 talk ”There’s Plenty of Room at the Bottom” in which he proposed the direct manipulation of individual atoms as a more powerful form of synthetic chemistry. Eric Drexler of MIT expanded Taniguchi’s definition and popularised nanotechnology in his 1986 book “Engines of Creation: The Coming Era of Nanotechnology”. On a nanoscale, i.e. from around 100 nm down to the size of atoms (approximately 0.2 nm) the properties of materials can be very different from those on a

INTRODUCTION

Molecular tools generally refer to molecular genetic tools. Although the first use of these tools began more than four decades ago, whenLewontin & Hubby (1966) used allozymes to find out the amount of genetic variation present in wild populations of Drosophila, the lack of expertise and high cost of these tools have prevented their widespread use among ecologists in general and behavioural ecologists in particular. But recently the accessibility and costs of these techniques have come down a lot and with the right kind of collaboration, lack of expertise is no longer an issue.

As was mentioned above, the Lewontin & Hubby’s (1966) investigation was done using allozymes. Although still an option, user-friendly neutral nucleotide markers are more preferred by investigators nowadays. The discovery of the polymerase chain reaction (PCR) in 1983 by Kary Mullis was the single landmark in this field, if one has to mention one. The use of molecular markers has totally revolutionized the field of ecology. It has provided ecologists the tool to ask questions, which were hitherto impossible to tackle and also to answer questions with much more precision and accuracy than was possible till now. It has toppled long held beliefs, sometimes, romantic ones, by ecologists.

Tissue Doppler Imaging

Doppler echocardiography relies on detection of the shift in frequency of ultrasound signals reflected from moving objects. With this principle, conventional Doppler techniques assess the velocity of blood flow by measuring high-frequency, low amplitude signals from small, fast-moving blood cells. In TDI, the same Doppler principles are used to quantify the higher-amplitude, lower-velocity signals of myocardial tissue motion.

There are important limitations to TD interrogation. As with all Doppler techniques, TDI measures only the vector of motion that is parallel to the direction of the ultrasound beam. In addition, TDI measures absolute tissue velocity and is unable to discriminate passive motion (related to translation or tethering) from active motion (fiber shortening or lengthening). The emerging technology of Doppler strain imaging provides a means to differentiate true contractility from passive myocardial motion by looking at relative changes in tissue velocity.

Precision medicine and personalised treatment of systemic or tumour diseases gain their acceptance and application in veterinary care, too. The application of personalised medical protocols and companion diagnostics is easier and ethically well reasoned within the veterinary medical field – and it can offer many advantageous lessons to human clinical transformation, too [1]. In vitro companion diagnostics for testing the applicability and sensitivity levels of certain kinase inhibitor and immunotherapeutic drugs offer a greatly increased efficiency for veterinary cancer patients, too. As the understanding of cancer biology has advanced, the field of veterinary oncology has grown significantly, paralleling developments in human oncology. Precision medicine is an innovative approach to disease treatment and prevention that considers individual variability in genes, environment, and lifestyle. In the context of veterinary medicine, precision medicine aims to provide tailored therapies that are specifically designed for the unique genetic and molecular profile of each animal’s cancer. This approach moves away from the traditional “one-size-fits-all” model, instead focusing on targeted treatments that maximize efficacy while minimizing side effects. Companion diagnostics, such as molecular tests, help determine whether a dog or cat is likely to respond to a particular drug. For dogs and cats, precision medicine holds great promise in improving outcomes for cancers, which are among the leading causes of death in companion animals.

As the heart diseases in companion animals is becoming common as in humans, the research in canine cardiac diseases is one of the most extensive studies in the present scenario. As per the available literature, the overall occurrence of various cardiac abnormalities ranges from 0.37 percent to 1.67 percent in various parts of the country. Whereas, in western parts of the globe, nearly 8 million dogs suffer from heart disease accounting 10% of all dogs in the United States. Heart diseases are common among older dogs; up to 75% of senior dogs have some type of heart condition, and unfortunately, most go undiagnosed due to varied reasons. The recognition of canine cardiac diseases has been delayed, and ignored on account of lack of awareness and knowledge by the owner and inadequate diagnostic facility in our country. Physical examination, radiography, electrocardiography and echocardiography are most useful diagnostic procedures employed in diagnosing the cardiac diseases in dogs. However, majority of the acquired cardiac diseases are being managed medically with one or a combination of drugs. Improved diet management and health care of companion animals have led to increased life expectancy, as a result, cardiac diseases have become pivotal causes of death for both dogs and cats. For such reason, a major objective has become to increase the quality of life by reducing the expansion of cardiac diseases via early detection of diseases and provision of effective treatment.

Introduction

Wound is a disruption to the epithelial integrity of the skin, extended in-depth to subcutaneous tissue and other body parts such as ligaments, muscles, blood vessels, nerves, and bone (Robson et al. 2001). An appropriate wound healing is essential for the restoration of disrupted anatomical continuity (Begum, 2000). We have observed increasing interest in research based on traditional medicines of the world in the recent era (Medina-Franco et al. 2013). Traditional and complementary medicine regains popularity not only in developing countries but also in developed countries. Common and known practices across the globe of traditional medicines include Herbal, Ayurveda, Siddha, Unani, Iranian, Islamic, Vietnamese, Chinese, Acupuncture, African and other pseudo-medical knowledge (Dorai 2012). According to World Health Organization (WHO), traditional medicine ‘the health practices, approaches, knowledge and beliefs incorporating plant, animal and mineral-based medicines, spiritual therapies, manual techniques and exercises, applied singularly or in combination to treat, diagnose and prevent illnesses or maintain well-being’. Saxena et al. (2013) reported that phytochemicals present in plants are liable for preventing disease and promoting health. Antibiotics used in the treatment of wound infections are now proved to have adverse effects in the human body and in pathogens where resistance is emerging. Attention has, therefore, turned towards extracting biologically active compounds from plant species having wound healing property (Pattanayak and Sunita, 2008).

Introduction

Animal behaviour is the study of how animals interact with one another, other living things, and their environment. Animals display a variety of behaviours in response to both external stimulus and their own pathophysiological state. Almost all domestic animals express a variety of behavioural responses throughout their lifespan, like feeding, sexual behaviours, etc. But every species responds to the same stimuli in a different way. This is why; the study of animal behaviour helps in efficient breeding, feeding, and reproductive management. It also serves as a reliable indicator of overall health and well-being.

Arteries of neck

Common carotid artery

The right and left common carotid arteries arise from the bicarotid trunk as it bifurcates on the ventral surface of the trachea at the thoracic inlet. In their course to reach the head, both of these, the right and left, common carotid arteries ascend through the neck along the ventrolateral aspect of the trachea and oesophagus respectively. During this course each of these arteries is accompanied by the vagosympathetic trunk dorsal to it, recurrent laryngeal nerve ventral to it and the internal jugular vein lateral to it. All these structures are enclosed in the carotid sheath. The external jugular vein is separated from the common carotid artery by the omohyoideus and sternocephalicus muscles. The right artery crosses the lateral surface of the trachea and passes along its dorsolateral surface and finally reaches to the lateral surface of the larynx and pharynx in front of the digastricus muscle ventral to the jugular process of the occipital bone. The left artery crosses the trachea and oesophagus and continues along its dorsolateral surface to the level of the third cervical vertebra. Here the oesophagus inclines towards the median plane and the artery follows the lateral surface of oesophagus till it reaches to the lateral surface of the larynx and pharynx just in front of the digastricus muscle and ventral to the jugular processes of the occipital bone. At this point the common carotid artery terminates by giving off the occipital, and pharyngeal arteries and then further continues as the external carotid artery. Along its course through the neck, the common carotid artery gives out several branches as below.