eChapters

Introduction
The development of the domestic phenotype mirrors the domestication process. Are certain behavioral patterns typically lost when animal are domesticated? Why do domesticated animals exhibit some behaviors more frequently than their wild counterparts? Darwin (1868) believed that in captivity biological traits are lost through disuse. Hemmer (1990) proposed that domestic animals have experienced a kind of regressive evolution, in which their behavior is less influence by environmental stimuli than that of their wild counterparts. The Earth’s rotation around its axis influences our physiology and behaviour. Animal species that are compelled to be active continuously typically exhibit poor performance, health, and survival. However, we examine the evidence of animals displaying extended periods of activity with weakened or nonexistent circadian rhythms and no observable
negative consequences. Available reports suggested that there is a greater prevalence of around-the-clock and ultradian activity patterns than commonly acknowledged.
This is especially true among herbivores, animals in Polar Regions and constant physical environments, animals in specific life-history stages, and highly social animals. Various mechanisms exist, but research indicates that certain circadian pacemakers still function in animals that are active throughout the day and night. The prevalence of continuous activity in various animals and habitats, along with a wide range of underlying mechanisms, suggests a pattern of convergent evolution. The circadian system’s organizational principles and complexity allow for potential chrono-biological plasticity. There could be compromises between the advantages of consistent daily patterns and adaptability, which, due to reasons that are not well comprehended, result in the functional suitability of irregular daily patterns only in specific environments and for specific ways of life.

1    Disease diagnosis based on posture, gait appearance and clinical signs

Introduction

Mineral imbalance in the animal body produces several problems. Normal dairy animals experience constantly changing nutrient demands and environmental stress during pregnancy and lactation. In the first part of the cycle the negative balance must be held to minimum, later the reserves must be replenished through fine tuned nutritional support for optimum production of the dairy animals. Inadequate blood calcium (Ca), phosphorus (P), magnesium (Mg), or potassium (K) concentrations can cause a dairy animal to lose the ability to rise to her feet as these elements are necessary for nerve and muscle function and are therefore of particular concern in the newly calved dairy animal. Mechanisms for maintaining blood status of these elements concentrations perform efficiently most of the time but occasionally these homeostatic mechanisms fail and metabolic diseases such as milk fever occur. Understanding about cause and predisposing factors behind the same may be helpful to develop strategies to avoid these disorders.

Macro elements have a significant role in normal functioning of the body, including proper reproduction and production. Imbalance of these elements in the body of a peri parturient animal creates severe problems to the animal body and even the productivity of the animal gets seriously affected. Among the macro elements calcium, phosphorus and magnesium with cation-anion balancing elements (sodium, potassium and chlorine) play an important role in normal health of dairy animals during the parturition period.

Introduction

"The new science of nutrigenomics teaches us what specific foods tell your genes. What you eat directly determines the genetic messages your body receives. These messages, in turn, control all the molecules that constitute your metabolism: the molecules that tell your body to burn calories or store them. If you can learn the language of your genes and control the messages and instructions they give your body and your metabolism, you can radically alter how food interacts with your body, lose weight, and optimize your health." (Mark Hyman 2006).

Nutrients have long been considered merely as fuel or cofactors however, it is accepted that diet and feeding strategies play an important role in determining the basic physiological processes that influence the health and wellbeing of animals and ultimately influence the efficiency of production. Traditional research related to animal nutrition mainly deals with either deficiency or excess of the particular nutrient which affects their health and productivity. Subsequently, it was appreciated that nutrients can themselves participate in the regulation of metabolic pathways, for instance through the allosteric regulation of specific enzymes, or by modulating hormone secretion. Shifting of balance between health and disease states involves the complex interplay of genes and the environment, which includes nutrients. The use of systems biology approach in nutrition has attracted both scientists and industrialists during recent decades and a link between diet and health is well established. However, it has often been difficult to easily evaluate the effects of specific nutrients, combinations of nutrients and nutrient interactions because of the lack of straightforward biological indicators that respond to even the simplest changes in nutrition or nutritional status.

1    Common diseases affecting livestock

2    Disease information

3    Diseases of skin

4    Diseases of muscuoskeletal system in canines

1    Large animals

2    Canines

3    Suggested antimicrobial/antifungal agents for intra-uterine administration

4    Commercial preparations of hormone(s) and recommended dosage protocol in cows

5    Drugs safe & contra indicated in pregnancy

Macroeconomic policy : Top-down policy by government and central banks, usually intended to maximise growth while keeping down inflation and unempoyment. The main instruments of macroeconomic policy are changes in the rate of interest and money supply, known as monetary policy, and changes in taxation and public spending, known as fiscal policy. The fact that unemployment and inflation often rise sharply, and that growth often slows or gdp falls, may be evidence of poorly executed macroeconomic policy. However, business cycles may simply be an unavoidable fact of economic life that macroeconomic policy, however well conducted, can never be sure of conquering.

Macroeconomics : The big picture analysing economy-wide phenomena such as growth, inflation and unemployment. Contrast with microeconomics, the study of the behaviour of individual markets, workers, households and firms. Although economists generally separate themselves into distinct macro and micro camps, macroeconomic phenomena are the product of all the microeconomic activity in an economy. The precise relationship between macro and micro is not particularly well understood, which has often made it difficult for a government to deliver well-run macroeconomic policy.

Managed Care : Managed care is a livestock health care plan that integrates the financing and delivery of livestock health care services by using arrangements with selected health care providers to provide services for covered individuals. Plans are generally financed using capitation fees. There are significant financial incentives for members of the plan to use the health care providers associated with the plan. The plan includes formal programs for quality assurance and utilization review.

Laboratory investigation of the samples assist in diagnosing, confirming the cases including veterolegal cases. The type of material, quantity of specimens & preservatives used and the method of dispatch to the laboratory  is tabulated in this chapter.

Pandemic : An epidemic occurring over a very wide area (several countries or continents) and usually affecting a large proportion of the population.

Partial Evaluation : Partial evaluations constitute a number of economic study types which consider costs and/or consequences, but which either do not involve a comparison between alternative interventions or do not relate costs to benefits.

Participation bias : People who participate in a study, especially volunteers, may be systematically different from those who do not, in a way that can affect the response to a treatment. For example, if volunteers are chosen to test the effects of a vaccine, one might find a lower incidence of disease in this population simply because they are more health conscious than those who would not volunteer for such a study.

Path dependence : History matters. Where you have been in the past determines where you are now and where you can go in future. Indeed, even small, apparently trivial, differences in the path you have taken can have huge consequences for where you are and can go. In economics, path dependence refers to the way in which apparently insignificant events and choices can have huge consequences for the development of a market or an economy.

The present study on trichology of domestic and wild animals was conducted in the Postgraduate laboratory of the Department of Veterinary Anatomy and Histol ogy, Nagpur Veterinary College, Nagpur. The study was undertaken with an aim of species differentiation from the guard hair of domestic as well as wild animals. The hair samples were collected from a total of eight domestic animal species and six wild animal species. Six animals from each species were studied and only three animals studied from the Sambar species as only three animals from this species was reported for post-mortem to the Nagpur Veterinary College, Nagpur within the period of research.

The annual milk production in Tamil Nadu during 2009-10 from crossbred cows, non-descript cows and buffaloes were 42.28,7.89 and 7.61 lakh tones, respectively. Tamil Nadu had 59.83 lakhs crossbred female cows in the year 2007 as against 41.47 lakhs in 2003 with an annual growth rate of 9.59 per cent (Anon, 2010). With the increasing crossbred cattle population and average annual production, better management of the high producers becomes a necessity. Production and health of animals depend mostly on environment in which they live. A satisfactory environment for any farm livestock is the one that ensures not only optimal productivity but also meets the health and behavioral needs of the animals. A satisfactory environment is the one that satisfies the following four criteria: thermal comfort, physical comfort, disease control and behavioral satisfaction. The most important environmental interventions done in recent days are those that have been done in housing and other attempts to ameliorate the thermal environment. The effort of the body to maintain a stable internal environment to challenges from widely variable environments was first described by Claude Bernard (1878) and later referred to as homeostasis (Cannon, 1932). An environment in which stressors are minimized would likely be favorable for efficient production of products derived from domestic farm animals and for helping ensure the well-being of those species.

Ruminants have evolved to consume and subsist on roughage - grasses and shrubs built predominantly of cellulose.The rumen is a fermentation vat par excellance, providing an anaerobic environment, constant temperature and pH, and good mixing. Well-masticated substrates are delivered through the esophagus on a regular schedule, and fermentation products are either absorbed in the rumen itself or flow out for further digestion and absorption downstream. Rumen gases, particularly methane, are increasingly in the news because of their contribution to greenhouse gas and climate change.

The ruminant stomach is composed of four parts; the rumen, reticulum, omasum and abomasum. These compartments gives the cow and other ruminants a decided advantage in digesting and utilizing those parts of the plants and other compounds which are practically useless to animals with simple stomach. Substances such as cellulose, a major constituent of plant tissues and urea, a non protein nitrogen compound, are of limited use of non ruminants. However ruminants through fermenting and synthesizing action of microorganisms in their complex stomach, can efficiently utilize these substances for productive purposes. The dairy cow converts this feed into milk and meat, two highly nutritious and palatable food for humans. This ability to make use of feed which otherwise would go waste because it is not directly consumable for humans is one of the most compelling justifications for the existence of cattle and buffaloes, in a world which must make use of all possible food resources.

Checks and Balances in Biotechnology Applications
    
Biosafety Issues in Animal Biotechnology Application
    
World Organization for Animal Health (OIE)
    
Recombinant DNA Safety Guidelines (1990)
    
Foreign Trade (Development and Regulation) Act, 1992 (2006)(Draft Amendment)
    
Revised Guidelines for Research in Transgenic Plants and Guidelines for Toxicity and Allergenicity Evaluation of Transgenic Seeds, Plants and Plant Parts (1998)
    
Guidelines for Generating Preclinical and Clinical Data for rDNA Vaccines, Diagnostics and other Biologicals (1999)
    
The Food Safety and Standards Act (2006)
    
Transgenic Animals Bioreactors
    
First Patent in Biotechnology
    
Biotechnology - Ethical Concerns
    
Some Priority Areas in the Field of Biotechnology
    
Suggestions for Future Development of Biotechnology Activities in the Field of Animal Health and Production in India
    
Epilogue

Animal Science Biotechnology Research Centres
    
Current Status and Some of the Achievements in Animal Heatlh
    
National Bioresource Development Board
    
Animal Biotechnology
    
Biotechnology Parks and Incubators
    
National Institute of Immunology, New Delhi
    
Bose Institute, Kolkata
    
Jawahar Lal Nehru University, New Delhi
    
National Centre For Cell Science, Pune
    
Centre for Dna Fingerprinting and Diagnostics (Cdfd) Hyderabad
    
National Institute for Plant Genome Research (Nipgr) New Delhi
    
Institute of Bioresources and Sustainable Development,Imphal
    
Institute of Life Sciences, Bhubaneshwar
    
Bharat Immunologicals and Biologicals Corporation Limited
    
International Centre for Genetic Engineering and Biotechnolgoy (Icgeb), New Delhi
    
Animal Biotechnology Research Abroad

1. Introduction
Good Laboratory Practice (GLP) is a systematic quality system essential for
ensuring the reliability, consistency, and integrity of laboratory data, particularly when the findings are used for regulatory decision-making. GLP ensures that laboratory studies are conducted in a scientific manner, maintaining high standards of ethical practice, precision, and reproducibility, with a primary focus on ensuring public and environmental safety. Introduced by the U.S. Food and Drug Administration (FDA) in 1978, GLP gained global recognition through the Organisation for Economic Cooperation and Development (OECD), which established guidelines for its implementation. GLP principles are now widely adopted in regulatory testing for pharmaceuticals, chemicals, food safety, and environmental testing. The OECD’s guidance on GLP has been instrumental in developing standards across the globe, ensuring that test results are scientifically valid and can be accepted internationally. In Asia, particularly in India and other developing countries, GLP compliance has gained increasing importance as pharmaceutical, environmental, and chemical industries expand. Regulatory bodies in these regions have gradually aligned their standards with international norms to ensure their competitiveness in global markets. This chapter discusses the key SOPs necessary for GLP compliance and provides a regional perspective, focusing on India and other Asian nations, where GLP frameworks are evolving to meet international standards.
2. Objective of GLP
The overarching objective of GLP is to ensure that laboratory data is accurate, reliable, and reproducible across different studies. The scientific and regulatory communities rely on GLP to ensure that findings from preclinical studies and safety evaluations can be trusted and utilized for regulatory approval.
The key objectives of GLP include
• Accuracy and Integrity of Data: GLP ensures that every aspect of data collection, analysis, and reporting is conducted with integrity and accuracy, preventing errors or falsification that could affect regulatory decisions.

The arid and semi-arid regions of India are drought prone and the availability vegetation of these regions is more acute during summer when the quality of the pastures becomes vulnerable. Small ruminants in the semi-arid regions need to adopt special physiological function to maintain thermal equilibrium. The reproductive efficiency of different breeds of sheep inhabiting in the semi-arid regions of India is relatively low (Arora and Garg, 1998).The nature of nutritional resources and management problems differs with climate, soil and vegetation, the physical process which governs the animals reproductive performance are the same; the female must reach puberty, show behavioral estrus and shed one or more ova which have then to be fertilized. Dietary nutrition promotes the programming and expression of the metabolic pathways that enable animals to achieve their genetic potential for reproduction. Nutrition has an important impact on the reproductive performance in sheep, but the magnitude of the effect on reproduction may vary with the season (White et al., 1983). Sheep are more prone to neglect compared to cattle and often suffer from lack of feed and fodder. The animals during the grazing are exposed to combined stress (Sejian et al., 2010 ab, and 2011) .This leads to decrease in body weight and loss of reproductive functions. Concurrently, Rhind and Mc Neilly (1986) showed that ewes with low body fat reserves had fewer large ovarian follicles and therefore the potential for subsequent maturation and ovulation declined. 

Reproduction in the males culminates in the production of semen containing fertile spermatozoa and deposition of these in the female reproductive tract at an opportune time.

Male Reproductive Organs

The reproductive organs in male domestic animals comprise the testes, epididymides, and accessory glands. The testes produce the spermatozoa and the male sex hormones (androgens). The spermatozoa pass from the testis into the epididymis where they acquire motility and attributes essential for fertilization and are then stored there. At ejaculation they are squeezed along the ductus deferens into the urethra and mixed with the secretions of the accessory glands to constitute the ejaculated semen.

Testis

The testis are composed of long tubes called seminiferous tubules and interstitial tissue. The spermatozoa are produced within the seminiferous tubules. This process is known as spermatogenesis. The interstitial tissue contains Leydig cells which are responsible for the production of the male hormone called androgens. The androgens are produced under the influence of hormones from the anterior pituitary namely ICSH (LH) and FSH. The principal androgens produced by the testis are testosterone and dihydrotestosterone.

The testis are suspended from the body in the loose sacs called scrotum, present in the inguinal region. The scrotum in addition to its container function, also acts as a regulator of temperature required for spermatogenesis.

Introduction
According to the U.S. Food and Drug Administration (FDA), Good Laboratory Practices (GLP) is a quality system that ensures the nonclinical safety studies are appropriately designed, conducted, and documented to generate reliable data. The laboratory techniques are defined as the processes and practices that are recommended for equipment use in the laboratory, while laboratory methods are based on established scientific principles involving biology, chemistry, and physics, and encompass all aspects of the clinical laboratory from testing the amount of cholesterol in blood to analysing DNA to growing microscopic organisms that may be causing an infection. One of the most significant parts of laboratory techniques and laboratory methods is the proper validation of the equipments (old or new equipments) as well as all the testing procedures/ methods (established or newly developed methods). Analytical method validation is a critical process to ensure the quality and safety of products. The objective of the validation of an analytical method is to demonstrate that it is suitable for its intended purpose; the results are authentic and universally acceptable. An analytical method is suitably well considered when the results generated through different method are consistent, reproducible, and thus, reliable.
The validation is required when
• A new method has been developed
• Revision of established method
• Use of established methods in different laboratories and different analysts, etc.
• Comparison of methods required
• When quality control indicates method changes

Bone

Bones provide the rigid framework of the body of animals and man. The skeletal system serves three main functions. (1) It supports and protects the soft tissues and organs of the body. (2) It helps to maintain, through homeostatic regulation, the constancy of the ionic environment within the organism. (3) It helps in locomotion with the help of muscles and tendons.

The ends of long bone is called the epiphysis: the long, compact shaft, the diaphysis where it joins the epiphysis, the metaphysis. The diaphysis is composed of a hollow cylinder which contained the medullary cavity and bone marrow. During postnatal life, erythropoiesis occurs mainly in the bone marrow. In the adult, bone contains on wet weight basis approximately 25% water, 45 Percent ash and 30 percent organic matter. With increasing age, the ash content progressively increases as the water content decreases; the organic fraction remains relatively constant. Calcium constitutes about 37% of the ash content, and phosphorus about 18.5%.