eChapters

Early reports are always path finders for future research. Earlier studies at CSIR-NBRI on characterization of bougainvillea is so important that it should be highlighted for researchers. Horto-taxonomical description of the leading Bougainvillea species/cultivars have been prepared according to their affinities to the species and hybrid seedlings (Sharma 1986a,b, 1996, Srinivas 1995, Khoshoo 1971a,b, Zadoo et al., 1975a, Jayanthi 1999). 
Abraham Kavoor : Bougainvillea x buttiana, tall, vigorous growth, young branches greenish-coppery, leaf blade ovate, green; thorn long, straight; bracts ovate, yellow-orange.
Abhimanyu : B. peruviana, bud sport of ‘Arjuna’, medium, drooping; branch greenish-coppery; leaves variegated, leaf elliptic, green; thorn long, straight, bracts elliptic, red-purple.
Aida : B. spectabilis, hybrid seedling of B. spectabilis (Lancaster 1940, Calcutta), tall, growth vigorous, young stem greenish coppery, tomentose, leaf blade ovate, base acute, apex acute to acuminate, coppery, old dark green, puberulent, thorn straight, bract young carmine changing to rose madder, elliptic, apex obtuse, profuse blooming, ideal as shrub, and pot culture.

Introduction
Antimicrobial resistance (AMR) has emerged as one of the most alarming public health challenges of the 21st century, threatening the effectiveness of life-saving drugs and the future of modern medicine. This crisis is not limited to healthcare settings; it spans communities, agriculture, and the environment, impacting every facet of society. India, with its large population, high burden of infectious diseases, unregulated access to antibiotics, and considerable pharmaceutical manufacturing industry, stands at the forefront of the global struggle against AMR. The rise of resistant microbes stems from a complex interplay of factors: overuse and misuse of antibiotics in humans and animals, poor infection prevention practices, lack of sanitation, and environmental contamination from antibiotic waste. As a result, common infections are becoming harder to treat, surgical procedures riskier, and healthcare costs are escalating, all leading to increased morbidity and mortality. Tackling AMR requires robust intersectoral collaboration—no single stakeholder can succeed alone. Non-governmental organizations (NGOs), industries, and Corporate Social Responsibility (CSR) initiatives form a critical triad in India’s fight against AMR. NGOs drive grassroots education, advocacy, surveillance, and policy change, often bridging gaps where governmental capacity is limited. The pharmaceutical and healthcare industries, responsible for antibiotic manufacturing, distribution, stewardship, and infection control, play a pivotal role in curbing environmental emissions, supporting responsible use, and promoting the discovery of new treatments. Meanwhile, CSR channels the resources and expertise of corporations towards sustainable, communityfacing projects that promote awareness, improve hygiene, foster stewardship, and drive systemic change.

The Millon Reaction
Tissues fixation in 10% neutral buffered formalin solution
Protocol
1) Deparaffinise the paraffin sections with two changes (10 minutes each) of xylene.
2) Descending grades of alcohol from absolute to 50% solution (2 minutes each).
3) Hydrate the sections with distilled water for 5 minutes.
4) Transfer the sections to a small beaker with the reagent and gently boil.
5) Stop the heating process and let the solution cool to room temperature.
6) Remove the sections and rinse them three times in distilled water (2 minutes each).
7) Dehydrate in ascending grades of 90% to absolute alcohol (one minute each).
8) Clear in xylene, two changes of 30 minutes each.
9) Mount glass cover slip with DPX.

There are three types of disease occurrence namely, endemic, epidemic and outbreaks. Martin et al. (1993) described the term endemic as the disease occurrence in a predictable pattern. There can be low, moderate or high levels of endemicity. Although many times the terms epidemic and outbreak are used synonymously, the term epidemic is used when there is an unexpected incidence of a disease. Thrusfield (1995) defined an outbreak as the occurrence of a disease involving one or more animals.

Animal disease prevention and control activities offer valuable benefits for food production, food security and safety, animal and human welfare, and alleviation of poverty. The effectiveness of disease prevention and control policies depends on the good governance and quality of the veterinary services, in compliance with OIE standards and guidelines on animal disease control. The apex body in each country is the core system that takes care of the prevention and control of animal diseases. This body is responsible for early detection of diseases and rapid response to outbreaks. The veterinary services in developing countries require adequate human and financial resources and capacity building to carry out the tasks of disease prevention and control and to protect animal health and thus public health, including food security and food safety.

Unna’s Method for Mast Cells
Tissues fix in 10% neutral buffered formalin and process for routine paraffin sections
Protocol
1) Deparaffinise the paraffin sections with two changes (10 minutes each) of xylene.
2) Descending grades of ethanol from absolute to 50% solution (2 minutes each).
3) Hydrate the sections with distilled water 5 minutes.
4) Polychrome methylene blue solution for 10 minutes.
5) Rinsing in distilled water.
6) Differentiate in glycerin-ether solution for 30 seconds to 1 minute.
7) Washing in water for 2-3 minutes.
8) Blotting with filter paper.
9) Dehydrate quickly in 100% ethanol.
10) Clearing in xylene, two changes of 30 minutes each.
11) Mount glass cover slip with DPX.

Electron-microscopy, as one of the several tools in diagnostic pathology and cell biology has contributed significantly to reveal several unimagined structures in modern era of science and technology. Several branches of biology and biomedical sciences like anatomy, pathology, physiology, biochemistry, microbiology rely on the transmission electron-microscope. This is used not only to visualize biological materials, but also to analyze the chemical makeup and physical properties of biological materials. Correlate electron microscopic and biochemical studies of cellular processes transcended the boundaries between structurally and functionally oriented sciences. The first electronmicroscope was developed by Max Knoll and Ernst Ruska in 1932 in Germany. Ruska’s efforts were appreciated by awarding him Noble Prize in Physics in 1986. The Siemens Corporation introduced the first commercial transmission electron- microscope in 1938-39 and its commercial marketing started in 1940 by different manufacturers like Hitachi (Japan), JEOL (Japan), Philips (Holland), Zeiss (Germany). Transmission electron-microscope projects electrons through a very thin section of tissue to produce a two dimensional image on a phosphorescent screen. The brightness of a particular area of the image is proportional to the number of electrons that are transmitted through the specimen. A resolution of 0.2 nm can be achieved in the transmission electron-microscope. The first electron-micrograph of biological tissue was published by Marton in 1934. Albert Claude and George Palade were awarded the Nobel Prize (1974) in the field of Medicine for their accomplishments in cell biology employing electron-microscopy

AMR is undoubtedly the most serious challenge ever faced by the world health community because of its multifaceted complex nature and its overwhelming impact over the human and veterinary communities. Livestock sector is no exception to have the serious and devastating impact from the emergence and spread of AMR. Because of a steady shift towards intensive farming and increased stocking density there has been increase occurrence of infectious diseases. Growing world population, rapid urbanization, and deforestation, shrinkage of grazing land all aided to this process. This leads to increased usage of antimicrobials in food animals – cause of concern for the scientific community as AMU is known the single most important driver of AMR.

Rodents and lagomorphs are two distinct groups of mammals, often mistaken for each other due to some shared physical characteristics. However, they belong to different taxonomic orders and possess several key differences

5.1. Rodents

They belong to the order Rodentia and the example species include rats, mice, squirrels, hamsters, guinea pigs, beavers, and porcupines. Order Rodentia is the largest and most diversified mammalian group, differing widely in size, behavior, feeding habits, anatomy, and physiology. Based on the anatomy and functional differences of masseter muscles, it is divided into 3 suborders viz., Caviomorpha, Myomorpha, and Sciuromorpha. Caviomorpha is also called hystrichomorpha and includes guinea pigs, chinchillas and degu. The suborder Myomorpha includes mice, rats, hamsters and gerbils. These animals have the strongest ability to gnaw among animals in all 3 suborders. Sciuridae is a family that includes small or medium size rodents. This family consists of tree squirrels, ground or medium size squirrels, and large size squirrels also known as marmots or prairie dogs (Cynomys ludovicianus). Smaller and less bushy tailed squirrels are known as chipmunks (Tamias striatus) and flying squirrels (Glaucomys volans).

Cytauxzoonosis is an emerging tick-borne disease of domestic cats and wild felids caused by the protozoa of the genus Cytauxzoon. The common clinical findings in affected cats are similar to other hemoparasitic infections namely anorexia, depression, anemia, vomiting, icterus, and high fever. The first reporting of feline cytauxzoonosis with four fatal cases was done by Wagner in Missouri, USA in the year 1976.

Bobcats (Lynx rufus) are recognized as the natural wild reservoir of Cytauxzoon felis with prevalence reaching up to 100% in some enzootic areas (Zieman et al., 2017). This infection is highly fatal in domestic cats with 97% mortality without treatment (Meinkoth and Kocan, 2005). However, subclinical infection in some cats has also been evidenced i.e., they were infected with C? felis but did not display the acute clinical signs of the disease before diagnosis (Brown et al., 2010 and Rizzi et al., 2015).

Several other wild felids apart from bobcats and domestic cats have been found to be affected by fatal or nonfatal Cytauxzoon sp. infection. These include lions, jaguar, cougars, pumas, ocelots, Iberian lynx, meerkats, European wild cats and Asiatic wild cat, panthers, and tigers (Jakob and Wesemeier, 1996; Rotstein et al., 1999; Yabsley et al., 2006; Andre et al., 2009; Filoni et al., 2012 and Furtado et al., 2017).

Cellular stains are the colouring compounds used to impart different colours to varied type of cellular components and help in the demonstration of different micro-organisms such as bacteria, viral inclusions, fungal hyphae etc. The staining techniques enhance the contrast of tissue sections at microscopic level including histological, histopathological and cytological grounds. Before applying the special staining techniques on tissue sections few points which should be taken care include:
• Use of appropriate volume, concentration and type of fixative for preservation of tissue samples.
• Use of fixative with better compatibility as per the nature of tissues to be preserved.
• The tissue sections collected should not be very thick (5 mm approximately).
• Staining solutions should be immediately prepared where so ever required.
• Tissues must be sectioned with an ideal thickness between 4-6 microns.
Different types of stains routinely used to demonstrate varied type of cellular components and microorganisms:
Grocott’s Methenamine Silver (GMS) Staining Method
The fungal hyphae can be better demonstrated with Grocott’s Methenamine Silver stain (GMS). The basis for colouration of fungus is entirely based upon the oxidation of chromic acid forming aldehyde from the cell wall constituents of fungus and thereby reducing the silver-hexamine complex. The silverhexamine complex leads to the reduction of nascent Ag ions and leading the blackening of fungus in tissue sections.

7.1. Introduction

Chemical restraint of insectivores is a technique used by veterinarians and wildlife biologists to immobilize small mammals that primarily eat insects, such as shrews, moles, and hedgehogs. It is essential for performing examinations, administering medications, and conducting research on these animals.

There are several factors to consider when chemically restraining insectivores:

• Species: Different species of insectivores may have varying degrees of sensitivity to different drugs.

• Age and health: Younger and healthier animals generally tolerate chemical restraint better than older or sick animals.

• Temperature: Insectivores are sensitive to temperature regulation, so it’s crucial to maintain a stable body temperature during restraint.

ABSTRACT

Tropical wet evergreen and tropical moist deciduous forests were the preferred habitats of the Brown-hawk Owl. Detection rate and response to the broadcasted calls were highest during the breeding season and in all the census points, more than one individual i.e. either in pair or three individuals were detected. Besides conspecific species, some heterospecific species were also found to respond to the broadcasted calls of Brown-hawk Owl, where the Brown-hawk Owl was not detected during the Initial Quite Listening survey. Moreover, approach of the sympatric species was observed only in the riparian strip and in rest of the habitats they responded only with calls. No significant seasonal difference was observed in the habitat utilization but seasonality was observed in the response and the approaching distance to the conspecific calls broadcasted. Additionally, the response to the conspecfic calls indicated that the owls were responding to the conspecfic calls throughout the year.

Renal failure is one of the most commonly presented conditions in veterinary clinics world over. Although the potential for recovery is favorable, most animals die due to multisystemic effects induced by azotemia. Hence, extracorporeal removal of endogenous and exogenous toxins is very essential for expanding the window of recovery. Hemodialysis has been used in treatment of veterinary patients for the last 50 years and is still one of the most expensive and technically demanding treatment modalities in veterinary medicine. It is an advanced extracorporeal renal replacement therapy which has recently transitioned from a clinical curiosity to a more advanced standard for management of acute kidney injury (AKI) and chronic kidney disease (CKD) in companion animals particularly dogs and cats.

In addition to eliminating selective toxins that cause AKI, regardless of renal function, hemodialysis is also effective in the removal of pharmacokinetically active soluble uremic substances in CKD to enhance life expectancy and quality of life (Singh, 2021). Other common indications of hemodialysis in veterinary medicine include fluid overload, dysregulated body fluid composition, drug overdose or intoxications (Foster, 2020; Singh et al?, 2024). Medical management alone is often insufficient to control the clinical signs and metabolic abnormalities associated with uremia when AKI is severe or when there is involvement of acute on chronic kidney disease. Consequently, animals are at risk of death within a short therapeutic window, despite the potential reversibility of AKI and the improvement of life expectancy in CKD.

Introduction

The cardiovascular system is comprised of the heart and blood vessels (arteries, capillaries, and veins). The heart pumps blood to blood vessels which allow blood to flow throughout the body. Both heart and blood vessels are work together to keep the blood circulating, ensuring the interchange of O₂, CO₂, electrolytes, and nutrients between the blood and the tissue fluids & cells, and removing waste products from the body via the excretory system.

The purpose of the study of the cardiovascular system is aimed at understand the pathophysiology of the heart and vascular system. A physical examination, arterial blood pressure, and electrocardiography are routinely employed to examine the cardiovascular system. The physical examination of the heart includes palpation, percussion, and auscultation.

Introduction

As India is the leading global economy with livestock contributing 4.66% in total GVA to Indian Economy (PIB, 2024) and ranks 1st in Milk Production, 2nd in Egg production, 5th in meat production (BAHS, 2024). Being the largest exporter of milk and meat in the world , this has led to the intensification of animal production due to the increasing demand of major livestock products (MLPs), which has led to an increasing overall use of antimicrobials. Antimicrobials are invariably used to preserve animal and public health, but also as growth promoters at a sub-therapeutic level. The overuse and misuse of antibiotics can contribute to antimicrobial resistance. The growing resistance to antimicrobial drugs could reverse the therapeutic benefits; animals’ resistance to antimicrobial drugs, reduces productivity and leads to economic losses. Unwanted antimicrobials residues may be present in products of animal origins, in animal waste contaminating soil and water and the environment. Seventy-five to ninety percent of antimicrobials used in livestock are excreted, mostly unmetabolized. AMR poses a serious threat to the safety and quality of animal origin food, food security and livelihoods. Unhealthy and unproductive animals are no longer able to generate food products of acceptable safety and quality for human consumption and can no longer contribute to income generation. Insufficient standards for residues in animal food products, reduces the livestock sectors potential of access to trade and increases public health risks (https://www.fao.org/antimicrobial-resistance/key-sectors/animal-production/ en/).

Horse

The skull of horse is also pyramidal in shape like that of ox but the base of the pyramid is less wide than that of the ox. It is shorter in length and narrower in width than that of the ox. The cranium is smaller externally, however, the cavity of cranium is larger than that of the ox. The nasal cavity is divided into two chambers by nasal septum. The floor of the nasal cavity is shorter and less concave than that of the ox. The left and the right nasal chambers are completely separated and do not communicate with each other. The horn core is absent.

The occipital bone is placed at the extreme caudal part of the skull, caudal condyloid foramen is absent, the basi occipital is longer and prismatic and the squamous occipital is crossed by a nuchal crest. The sphenoid bone has two parts, the pre and post sphenoid, as that in case of ox. The wing of the post sphenoid presents three notches, carotid, oval and spinosal for internal carotid artery, ventral maxillary nerve and spheno-spinous artery respectively. The foramen orbitale and the foramen rotundum are not united. There are no major differences in the ethmoid bone of ox and horse. The frontal bones are less expansive than those of ox. These are situated between parietals caudally and nasals rostrally. The supraorbital foramen perforates the root of the supra orbital process that joins the zygomatic process of the squamous temporal. The horn core is absent. The ventral borders of the frontal bones of the opposite sides form a projection which fits into the notch formed by the two nasal bones. The temporal bone in horse has three parts, the squamous, petrous and tympanic as against two parts, the squamous and petrous of the ox. The squamous part is much more developed than that of ox. It has three parts, the body, zygomatic process and the occipital (pyramidal) process. The later one arises from the caudal part of the squamous. The stylomastoid foramen is between the hyoid and the mastoid processes and the auditory bulla is smaller. The interparietal is a single bone as that of ox.

Mouth

The mouth is bounded dorsally (roof) by hard palate (2-5 B) and behind that the soft palate, ventrally (floor) by the body of the mandible along with the mylohyoide muscles and laterally by the cheeks (Fig. 2-5 C). The entrance of mouth cavity is bounded by lips and it is called as rima oris, while its caudal opening into the pharynx is called as aditus pharyngis or isthmus faucium. The cavity of the mouth (cavum oris) is subdivided into two parts by the teeth and alveolar processes (the alveoli) of the mandible. The inner part i.e. the space within the teeth and alveoli is termed as mouth cavity proper, while the space between the cheeks and lips and that between the teeth and alveoli is called the vestibule of the mouth. The mucous membrane of the mouth cavity is continuous with the margin of the lips and the common integument and caudally it continues with the mucous membrane of the pharynx.

Introduction

Food security is defined as "a situation that exists when all people, at all times, have physical, social and economic access to sufficient, safe and nutritious food that meets their dietary needs and food preferences for an active and healthy life" (The State of Food Insecurity, 2001) (see http://www.who.int/trade/glossary/story028/en/). As of 2015, the concept of food security has mostly focused on food calories rather than the quality and nutrition of food. Thus, food security usually emphasizes food quantity and therefore "nutrition security" is used for the quality dimension. The concept of food security has four dimensions: availability, accessibility, utilization and vulnerability. The interactions among them determine how food secure a country is or how adequate, steady and risk-free food consumption is at the household and individual levels (Kumar and Ayyappan, 2014). Though substantial progress has been achieved in reducing food insecurity, maintaining food security at the national and household level is a major challenge for India (Singh, 2014). The present article briefs about global and Indian scenarios on food security aspects, and the key role of livestock in achieving zero hunger by means of livestock-derived foods such as milk, egg and meat products.

Livestock poisoning by plants is often a result of management errors, range conditions, or the type of animal, rather than just the presence of poisonous plants. Forage availability can be influenced by drought, overgrazing and snow storms, or altered grazing patterns. Animals can become overly hungry during transportation or other management activities that deprive them of food and water for extended periods of time, thus making them more likely to graze toxic plants. Grazing during the wrong season may compromise an animal's ability to select adequate and non-toxic forages (James, 1994).

The toxicity of plants varies with environmental factors such as site, temperature, precipitation, and light, time of day and stage of growth. For example, mature oak leaves are less toxic than young leaves. Cyanogenic plants such as arrow grass are more toxic following a frost or drought condition. Nitrates can be higher in plants following heavy nitrogen fertilisation or during a drought. Certain animal factors must also be considered in livestock poisoning by plants. For example, goats are considered to be comparatively resistant to lantana poisoning than cattle. A hungry animal is more susceptible to poisoning than a full one. A full rumen slows consumption and also dilutes the toxin. Though the animals are exposed to a large number of toxic plants when they are left for grazing, five will be discussed in detail in this chapter. These include lantana, ageratum, eupatorium, oak and ferns (James, 1994).

ENZYMES

Enzymes are the heart of Biochemistry

Protein based catalysts.

Enormously effective catalysts: typically enhance rates by 10⁶ to 10¹² fold.

Operate under mild conditions: 0 - 100 °C (or perhaps even 300+ °C for some bacteria in deep ocean), 20 - 40 °C for most organisms; and low pressures (atmospheric).

Very specific: Generally catalyze reaction for a very restricted group of molecules, sometimes for a single naturally occurring molecule of a single chirality.