eChapters

Reproductive Biotechnology
    
Artificial Insemination (AI)
    
History and Future Perspectives of Artificial Insemination
    
Semen Cryopreservation
    
Buffalo
    
Goat
    
Cryoprotection and Anti Oxidants
    
Cryopreservation and Genetic Stability of Livestock Spermatozoa
    
Insemination Technique
    
Cattle and Buffalo
    
Sheep and Goat
    
Conception Rate with Artificial Insemination
    
Multiple Ovulation and Embryo Transfer (MOET)
    
Advantages of Embryo Transfer
    
MOET Includes the Following Steps
    
Superovulatory Treatments
    
Insemination
    
Recovery of Embryos
    
Synchronization of Recipients
    
Cattle
    
Buffaloes
    
Goats
    
Horses
    
In vitro Maturation, fertilization and culture of Oocytes
    
Historical Development
    
In Vitro Maturation of Oocytes
    
In vitro Sperm Capacitation
    
Scope
    
Limitations
    
Embryo Cryopreservation
    
Ultrasonography in Reproductive Management
    
Ultrasound-guided Transvaginal Oocyte Pick-up (OPU)
    
Embryo Cloning or Nuclear Transfer
    
Blastomere Separation
    
Embryo Splitting
    
Nuclear Transfer Cloning (NT)
    
Embryonic Stem Cell Complementations
    
Limitations and Future Prospects of Cloning
    
Sexing of Embryos and Separation of X and Y Chromosomes Bearing Sperms
    
PCR  in Sex Determination
    
Transgenic Animal Production
    
Gene Transfer Through Direct DNA Microinjection
    
Gene Transfer with Retroviral-Vector Assistance
    
Gene Transfer through Production of Germ-line Chimeras
    
Parthenogenetic Embryos: An Emerging Tool in Reproductive Biotechnology for Multiplication of Superior Germ Plasm
    
Applications of Parthenogenesis
    
Livestock Genomics and Marker Assisted Selection (Use of DNA Level Markers)
    
Stem Cells
    
Vaccine for Fertility Control and Increase Prolificacy
    
Auto-immunization to Increase Prolificacy
    
Biotechnology of Conservation of Livestock Breed Biodiversity
    
Gene for Prolificity: Booroola Merino
    
Role of Biotechnology in Enhancing Production
    
Milk
    
Biotechnology of Rumen Manipulation
    
Genetically Modified Ruminal Microorganisms

Biotechnology of Animal Feeding
    
Probiotics
    
Biotechnology Crops as Animal Feed
    
Biotechnology of Animal Products
    
Some New Products made Possible by Biotechnology Include

Animal Science Biotechnology Research Centres
    
Current Status and Some of the Achievements in Animal Heatlh
    
National Bioresource Development Board
    
Animal Biotechnology
    
Biotechnology Parks and Incubators
    
National Institute of Immunology, New Delhi
    
Bose Institute, Kolkata
    
Jawahar Lal Nehru University, New Delhi
    
National Centre For Cell Science, Pune
    
Centre for Dna Fingerprinting and Diagnostics (Cdfd) Hyderabad
    
National Institute for Plant Genome Research (Nipgr) New Delhi
    
Institute of Bioresources and Sustainable Development,Imphal
    
Institute of Life Sciences, Bhubaneshwar
    
Bharat Immunologicals and Biologicals Corporation Limited
    
International Centre for Genetic Engineering and Biotechnolgoy (Icgeb), New Delhi
    
Animal Biotechnology Research Abroad

1    Ruminants
2    Canines

Acetic acid            
    

Molecular weight    60.05        
    Melting point (^ºC)    17        
    Boiling point (^ºC)    118        
    Flash point (^ºC)    40        
    Auto-ignition point (^ºC)    426        
    Specific gravity at 20^ºC    1.05        
    Molarity of the concentrated acid (M)    17.4    
    1 M solution    57.5 ml concentrated acid per litre

Introduction

Rainfed agro-ecosystems occupy a considerable place in Indian agriculture, covering 80 M ha in arid, semi-arid and sub-humid climatic zones; constituting nearly 57% of the net cultivated area. Rainfed areas contribute almost 100% of forest products, 84-87% of coarse-grain cereals and pulses, 80% of horticulture, 77% of oilseeds, 60% of cotton and 50% of fine cereals including rice, wheat, maize, sorghum etc (Srinivasarao et al., 2010; 2011). Rainfed regions support 60% of livestock, 40% of human population and contribute 40% of food grains and several special-attribute commodities such as seed spices, dyes, herbs, gums, etc. (Srinivasarao et al., 2011). It is estimated that even after achieving the full irrigation potential, barring for successful completion of river linking project, nearly 45 to 50% of the total cultivated area will remain dependent on rain. Undoubtedly, the rainfed agriculture would continue to occupy a prominent place in Indian agriculture for a long time to come (Venkateswarlu et al., 2011). Despite the progress made so far, rainfed agriculture in India encounters multiple risks and constraints relating to biophysical and socio-economic issues. Agricultural productivity in rainfed areas continues to remain low and unstable due to weather aberrations, nutrient disorder and poor socio-economic status of farmers.

Recent increase in extreme climate events threatens disruptive impacts on both animal and agriculture (Battisti and Naylor, 2009). Climate change is a global phenomenon, but its negative impacts are more severely felt by the poor people in developing countries. Moreover, rural poor communities rely greatly for their survival on animal and agriculture which belong to the most climate-sensitive economic sectors. Climate change is expected to intensify existing problems and create new combinations of risks. The incidences of droughts, snow-storms and blizzard like events have increased rapidly. The situation has further worsened due to factors such as widespread poverty, over dependence on rain-fed agriculture, inequitable land distribution, limited access to capital and technology, inadequate infrastructure, long term weather forecasts and inadequate research and extension. The impact of climate change is expected to heighten the vulnerability of animal systems and reinforce existing factors that are affecting animal production systems in many parts of the world.

Climate change has become a global threat as its impact is evident on the Earth. In the coming decades,the severity of climate change is expected to aggravate the ecosystem. Due to its vulnerable ecosystem and geographical location, the North eastern region (NER)of India that is home to about 40 million people is considered as one of the most climate sensitive areas in the country. The NER is among India’s least developed region andscarce information is available on the impact of climate change in this region. However, initial scientific research and field observations confirm that the region is suffering from the impacts of climate change already. The region comprising 0.26 million km2area occupying 8% of nation’s territoryconsists of the states of Assam, Arunachal Pradesh, Manipur, Meghalaya, Mizoram, Nagaland, Tripura and Sikkim. These states consist of a part of the east Himalayan region, which extends eastwards from Arunachal Pradesh to the Darjeeling hills of West Bengal. The entire region is a part of Indo-Burma and Himalayan hotspots i.e. characterized by rich biodiversity, heavy precipitation and high seismicity.

Global warming is the biggest long term threat to life on earth. Rise in temperature may drive thousands of species to extinction, trigger more frequent floods and droughts and sink low lying islands and coastal areas by rising sea levels. It is the result of rising atmospheric content of CO₂ mainly owing to burning of hydrocarbons or fossil fuels like as petrol and diesel. Destruction of forests and their degradation too contribute to rise in carbon dioxide levels. The IPCC (2006) projected the rate of warming for the 21st century to be between 0.8 and 4.4^ºC at various stabilized CO₂ levels in atmosphere and it is most likely to be 3°C by the end of this century. It could cost global economy almost $7 trillion by 2050, is equivalent to a 20% fall in growth if no action is taken on greenhouse gas emissions. If action is taken, it will cost only $350 billion due to climate change already taken place, just 1% of the global GDP. The winter 2007 was the warmest and recorded 0.85^ºC above average of 12oC and the previous highest was 0.71^ºC, which occurred in 2002 in Northern Hemisphere. The entire Europe Union recorded the warm winter, having more than 2^ºC above average. New York experienced the highest temperature of 21.7^ºC on a day in January, 2007 and the second highest was recorded as 17.2^ºC in 1950. The year 2007 was the warmest winter in the NHS. However, floods and excess rains were also noticed due to hurricanes and tropical storms worldwide in 2007. The year 2010 was the warmest year in India, followed by 2009. It was the second warmest year globally after 1998. It was also one of the wettest years globally in recent years and it was a landmark in annals of climatology.

Introduction

Increase in atmospheric carbondioxide (CO₂) and other green house gases viz. methane (CH₄), nitrous oxide (N₂O) and CFC (Chloro-Flouro- Carbon) due to fossil fuel burning, rapid industrialization and deforestation, which reduces the amount of earth’s radiation, that escapes to space. Role of these gases to change in climate over a longer period of time is the key issue of climate change. Climate change is a change in climate over time, whether due to natural variability or as a result of human activity (IPCC 2007).

IPCC :The UNEP (United Nations Environment Programme) and WMO (World Meteorological Organization) jointly established IPCC (Inter-governmental Panel on Climate Change) to assess available scientific information on climate change as well as to study its impact during 1988. Since establishment, IPCC has produced five major assessment reports. According to IPCC, climate change is defined as a movement in climate system as a result of internal changes within climate or interaction among its components. Frequency of extreme events (viz. heat waves, intense rain, floods, droughts etc.) are reported to follow increasing trend. Fig.1 is showing natural climatological cycle in the earth throughout different seasons as various components of climate system (IPCC 1996).

Livestock farming is an integral part of crop farming and contributes substantially to household nutritional security and poverty alleviation through increased household income. Indian agriculture is an economic symbiosis of crop and livestock production with cattle as the foundation. Dairy animals produce milk by converting the crop residues and by products from crops which otherwise would be wasted. Dairy sector contributes by way of cash income, drought power and manure. Livestock provides for human needs by way of food, fibre, fuel, fertilizer, skin and traction. India has the largest livestock populations in the world. It has 57 per cent of the world’s buffalo population and 16 per cent of the cattle population. It ranks first in respect of cattle and buffalo population, third in sheep and second in goat population in the world. 70 per cent of the livestock are owned by 67 per cent of small and marginal farmers. 76 per cent of the milk is produced by weaker sections of society. Out of total livestock in the country, 37.28 per cent are cattle, 21.29 per cent are buffaloes, 12.71 per cent are sheep, 2640 per cent are goats and only 2.01 per cent are pigs. All other animals are less than 0.37 per cent of the total livestock. The species-wise breakup of livestock population in India (Table 16.1)

1    General line of treatment of poisoning in animals

2    Systemic antidotes-dosage and method of treatment large animals

3    Small animals

4    Plant toxins

5    Zoo toxins

Livestock have the potential to be transformative; by enhancing food and nutrition security and providing the income to pay for education and for other needs, livestock can transform lives of many (Jimmy Smith et al., 2013). The projected growth of population is 9.6 billion in the year 2050 from the current population of 7.2billion (Gerber et al, FAO, 2013). The world demand for milk and meat in 2050 is expected to expand 58 and 73% respectively from their demand levels in 2010 (FAO, 2011). Nevertheless, the growth animal agriculture is not on par with the demand. Today, in a world of limited resources, in the face of climate change and a rising world population, a major issue is global food security (Augustine et al 2013). The key inferences of the latest approved summary for policymakers by the IPCC (2013) on climate change are 1). Warming of climate system is unequivocal and will continue beyond 2100. Global surface temperature change for the end of the 21st century is expected to exceed 1.5°C relative to 1850 to 1900 for almost all RCP scenarios 2). It is very likely that frequency of heat waves and heavy precipitation events have increased 3).

Conservation Agriculture (CA) is defined as a process of ‘sustainable intensification of agriculture’ for enhancing and sustaining production at low cost, without deteriorating soil health and flow of environmental services. The process of agricultural intensification increases food production and spares land for the other uses including eco-system services. In line with the vision of Norman Borlaug, agricultural intensification is also known as ‘The Borlaug Effect’. Frameworks such as ‘Conservation Agriculture’, ‘Ecological Intensification’ and ‘Ever Green Revolution’ share a common view of cropping systems as agro-ecosystems designed to make maximum use of fixed resources (land, light and temperature etc.) with optimum use of agri-inputs for attaining sustainable production levels (Cassman 1999)’. Conservation agriculture is also considered as an important component of the overall strategy for ensuring food security, poverty alleviation, health for all, rural development, enhancing productivity, improve environmental quality and preserve natural resources. With limited scope for further expansion of area under agriculture, production gains can be accomplished through intensification of agriculture, achieved by pursuing one or more strategies (with rationale use of natural resources as well as external production inputs) enumerated as under:

In India, being predominantly an agricultural country, livestock is emerging as a driving force in the growth of agricultural sector. Contribution of livestock to agriculture GDP has been rising steadily during the last three decades from 13.88% in 1980-81 to 27.28% in 2010-11 (BAHS 2012). About 70% population of the Country is engaged in agriculture and more than 50% of people below poverty line are associated with livestock production. India is blessed with a rich genetic resource of bovines, with an estimated number of 304.76 million (Livestock Census, 2007), which is 19% of the world. India continues to be the highest milk producing country in the world with an estimated production of 121.8 million tonnes during 2010-11 of which more than 53% is contributed by buffaloes (105.34 millions). Similarly, India ranks first in buffalo, second in cattle and goat, third in sheep, fourth in duck, fifth in chicken and sixth in camel population of the world. India is the third largest egg producer in the world (BAHS 2012). India posses a large treasure of domestic animal biodiversity with 37 breeds of cattle, 13 breeds of buffalo, 23 breeds of goat, 39 breeds of sheep, 6 breeds of horse/pony, 8 breeds of camel, 2 breeds of pig and 15 breeds of chicken registered under National Bureau of Animal Genetic Resources, Karnal.

Introduction

Agriculture is the mainstay of livelihood activity of about 70% of the state’s population. Madhya Pradesh is an agrarian state and the state’s economy is highly dependent on agriculture. Considering the sector’s sensitivity to climatic parameters it can be easily understood that the sector would be highly impacted by any change in these parameters.

Madhya Pradesh has a subtropical climate with three distinct seasons’ viz. winter from December to February, followed by summer season from March to May and rainy season extending from June to October. During winters the mean temperature remains around 10ºC and mean maximum temperature remains 25ºC and the minimum temperature goes down to 1°C in some regions. During summers, the mean minimum temperature is 22ºC and mean maximum is 38ºC. The maximum temperature during summer can go up to 48ºC, especially in May and June which are the hottest months.

1    Dental formulae and eruption of tooth

2    Ageing based on rings of the horn

3    General appearance of animal

4    Longevity of different animals

1    Bovines

2    Canines

1    Disease diagnosis based on posture, gait appearance and clinical signs

1    Common diseases affecting livestock

2    Disease information

3    Diseases of skin

4    Diseases of muscuoskeletal system in canines

8.1 Sub Cloning
    
8.2 Cloning into a Yeast Artificial Chromosome (YAC)
    
8.3 DNA Libraries