eChapters

Introduction

Nutritive value of any food indicates the calories content as well as quality attributes. Macronutrients such as carbohydrates, fats and proteins provide energy by4-9-4 rule. It meanstheyprovide 4,9 and 4 kcal/genergy, respectively, when oxidized completely. Though micronutrientssuch as vitaminsandminerals do not provide any calories, they are essential for the enzymatic activity and metabolic reactions.

ATP or energy is measured in terms of ‘calories’ or ‘joule’. One calorie is the amount of heat required by 1g of water to increase its temperature from 14.5 to 15.5°C under standard conditions. Nutritive value of any food can be measured on shelf usingcalorimeter, in which food is completelyoxidized under specified conditions and converted into CO₂ and water.

Introduction

Agroforestry offers both adaptation and mitigation options to climate change especially for the poor rural communities in the developing world. It is now getting ample attention from different investors, scientists, policy makers and international bodies due to its multiple benefits to the environment; and remain an important component of sustainable land use in agricultural production. Agroforestry provides economic, environmental, and social security to the vulnerable communities who are dependent on agricultural production for their livelihoods, and on forest/ trees for their energy requirement. Agricultual production is vulnerable to climate change, and there is a need to cushion these communities to be less vulnerable to climate change, or natural hazards. The suitable, sustainable and smart method is agroforestry based agriculture which offer host of ecosystem services to cope the vagaries of climate change, particularly in rural areas.

Climate change is a statistical variation in properties of the climate system that includes changes in global temperature, precipitation, etc due to natural or human drivers over a long period of time. It may affect distribution and quality of natural resources thereby influencing livelihood security of the people (Status of Indian Agriculture, 2013). Recent climate change activities is the increase in earth’s temperature by 0.74^ºC between 1906 to 2005 (IPCC 2007) due to increase in anthropogenic emissions of green house gases such as carbon dioxide (CO₂), methane (CH₄), ozone (O₃), nitrous oxide (N₂O) and chlorofluorocarbon (CFCs). Increase in concentration of green house gases influence future climate as well as agriculture (Mall et al., 2006; IPCC, 2001; Aggarwal, 2003: Bhatia et al., 2004). For Indian region under South Asia, Intergovernmental Panel on Climate Change (IPCC), 2007 projected a rise in temperature from 0.5-1.2^ºC by 2020, 0.88-3.16 oC by 2050, and 1.56 - 5.44^ºC by 2080. This may anticipate greater instability in agricultural production (Aggarwal, 2008). Beside this, climate change also affects the dynamics and frequency of extreme events like tropical cyclones, associated storm surges, and rainfall events. Low lying regions are more vulnerable to climate change as increase in temperature melt down the glaciers causing floods, submergence, and degradation of these regions. Therefore, there is a need for a ‘wake up call’ at this stage to aware farmers about the climate change, extreme weather events like hailstorm, heat and cold waves, drought, flash and river floods and landslides, and its affect on crop and agriculture, and strategies to overcome it.

Introduction
Ecological behavioral responses encompass a diverse array of adaptive behaviors exhibited by organisms in response to their dynamic environments. This interdisciplinary field integrates concepts from ecology and behavioral science to unravel the intricate ways in which organisms interact with and respond to their surroundings. This abstract provides a concise overview of key aspects within this burgeoning field.Firstly, behavioral
responses to environmental stimuli are essential for the survival and reproduction of organisms. From foraging strategies and predator avoidance to mating rituals and territorial defense, the behaviors exhibited by individuals are often finely tuned to the ecological challenges they face. Understanding the mechanisms underlying these responses sheds light on the evolutionary processes shaping behavioral adaptations.Furthermore, the role of
communication in ecological behavior cannot be overstated. Species communicate through a variety of means, including visual displays, vocalizations, chemical signals, and tactile interactions. These communication strategies facilitate intra and inter-species interactions, influencing mating choices, warning of potential threats, and establishing social hierarchies. Another critical aspect of ecological behavioral responses is the ability of organisms to adjust their behaviors in the face of environmental changes. Whether prompted by alterations in climate, resource availability, or human-induced disturbances, organisms exhibit plasticity in their behaviors. Such adaptability is crucial for species survival and underscores the importance of considering behavioral responses in conservation efforts and environmental management.Moreover, the study of behavioral responses provides valuable insights into ecosystem dynamics.

1    General line of treatment of poisoning in animals

2    Systemic antidotes-dosage and method of treatment large animals

3    Small animals

4    Plant toxins

5    Zoo toxins

Heat or thermal stress occurs when it becomes increasingly difficult for dairy cow to maintain a normal core body temperature of 101.5 to 102.8° F (rectal temp.). The thermo neutral or comfort zone (TNZ) for cow is at an environmental temperature of 41 to 77° F. Within the TNZ, heat production from normal metabolic functions is about equal to the heat loss from the body and maintaining a normal body temperature is relatively easy. Extent of heat stress can be measured by the Temperature Humidity Index (THI). A THI level above 75 is considered stressful for dairy animals. Two sources of heat impact the cow as the environmental temperature and the heat produced internally from basal nutrient metabolism. Heat produced from nutrient metabolism is a lesser factor than environmental heat sources. However, as milk production and feed intake increase, more heat from nutrient metabolism is produced aggravating any heat stress being incurred from environmental sources. Therefore, higher milk producing cow will be more affected by heat stress than low milk producing or dry cow. 

The Fourth Assessment Report of the United Nations Intergovernmental Panel on Climate Change (IPCC), after analysis of more than 6000 peer reviewed publications has assessed potential effects of changing climate on various spheres of life (Solomon et al., 2007). Under different scenarios, the estimated temperature rise of 1.1 to 6.4 °C in the global surface temperature is predicted at the end of 21st century (period 2090-2099 relative to 1980-1999). Global average sea level in the last interglacial period (about 125,000 years ago) was likely 4 to 6 m higher than during the 20th century, mainly due to the melting of ice in the polar regions. It has been detected in changes of surface and atmospheric temperatures in the upper several hundred metres of the ocean, and in contributions to sea level rise (Solomon et al., 2007). The change in climate is concurrent with the changes in global levels of carbondioxide, methane, oxides of nitrogen etc, loss of biodiversity, natural habitat and other natural resources. The IPCC report also estimates a confidence level of 90% that there will be more frequent warm spells, heat waves and heavy rainfall and a confidence level of 66% that there will be an increase in drought, tropical cyclones and extreme high tides. The magnitude of the events will vary depending on the geographic zones of the World (Solomon et al., 2007).

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Laboratory Safety and Hazards

Body needs energy for the functioning of various processes for the nutrition and repair of damage tissue. This energy comes from the food nutrients. The food nutrients undergo a series of chemical reactions in the body to release the necessary energy.

The higher animals are dependent on plants and other animals for their nutrients. They can classified into two groups.

Carnivores : Those animals which obtain most of their food by eating other animals are carnivores in these species digestion is mainly enzymatic in nature and microbial digestion is minimal.

Herbivores : These animals obtain most of their food nutrients from herbage and other plant parts. In the herbivores, microbial digestion is of great significance and allows utilization of plant materials that are not broken down by the enzymes present in the digestive secretions.

Most of the domesticated animals except cat & dog fall in the category of herbivores. The domesticated herbivores can be divided into three groups:

1. Ruminants : Those possessing a rumen (cow, buffalo, sheep, goat, camel etc.) in which there is extensive microbial fermentation of the herbage diet in a specialized region of the digestive tract, prior to its digestion by the alimentary enzymes.
2. Psedoruminants eg members of the camelidae family eg camel, Ilama, alpaca, vicuna etc. In pseudoruminants animal third compartment of the compound stomach is absent.
3. Non-Ruminant : The second group is those with simple stomachs (Horse, Pig) in which fermentation takes place in the posterior part of the digestive tract.

The demand for agricultural and livestock products is rising and the challenge to feed 8 billion people in 2020 is considerably enormous. Further, it is also hard to imagine meeting 2050-projected demand by raising twice as many poultry, 78% more small ruminants, 58% more cattle and 37% more pigs, without further damaging natural resources (Rivera and Lopez, 2012). Hence, sustainable development based on balance of ecology, economics, norms and values are to be considered at various levels of the scale: between food and farming systems, regions, nations and continents (Zipp, 2003). The food systems encompass activities related to the production, processing, distribution, preparation and consumption of food; and the outcomes of these activities contributing to food security (Fig. 14.1).The interactions between and within bio-geo-physical and human environments influence both the activities and the outcomes.

The reproductive system of female domestic animals consists of (1) A pair of ovaries attached with ligaments to the uterus (2) A pair of fallopian tubes the distal ends of which (infundibula) serve to receive ova expelled from the ovary and the proximal ends enter the uterus (3) The uterus proper with its outer smooth myometrium and inner mucous endometrium which either sloughs off periodically or develops in part into a placenta, depending on whether pregnancy occurs (4) Vagina which serves to receive sperm.

Ovaries

The two ovaries are connected to the back of the female with ligaments inside the main body cavity. When puberty, or sexual maturity is reached, the ovaries begin to function with a rhythm or cycle of activity, the most evident manifestation of which is estrus or heat. The estrus period or heat is the period when the female is receptive to the male. The duration of heat in cattle is around 19 hours. The interval between heats in 19½ to 20 days in cattle. The duration of estrus and cycle is variable in different individuals and in different seasons of the year.

The ovarian parenchyma contains a large number of potential egg forming cells. There are many more of these than are required for the breeding life of female. New follicles begin to grow each estrus cycle one of which finally ovulates.

The ovary is covered by the peritoneum except the attached border where the vessels and nerves enter. In most animals the ovary is some what divided into a cortex, in which the follicles with the developing ova are located and a medulla which is rich in blood vessels, lymphaticus and nerves.

Each Graafian follicle normally contains only one developing ovum. The follicle vary in size according to their stage of development, the more advanced project the surface of the ovary slightly.

Large Ruminants Neurological examination of farm animals is an essential part of the physical examination, especially when there’s a suspicion of neurological disease. The examination begins with observing the animal’s gait and how it interacts with its surroundings. The main goal is to determine whether the neurological issue is primary (originating in the nervous system) or secondary (caused by something else) and to pinpoint where the problem is in the nervous system ( central nervous system -CNS or the peripheral nervous system –PNS ). When examining an animal, it is best to start with procedures that cause the least distress. Painful tests or more invasive checks should be done at the end of the examination.

1. Introduction:- Ensuring Quality and Success in Assisted Reproductive
Technology through Good Laboratory Practices Assisted Reproductive Technology (ART) in animals has become an increasingly vital set of techniques with wide-ranging applications across agriculture, wildlife conservation, and biomedical research. These technologies, encompassing procedures such as in-vitro fertilization (IVF), embryo transfer, and cryopreservation of gametes and embryos, offer solutions to infertility, enable genetic improvement in livestock, and play a crucial role in preserving endangered species. As the application of ART expands throughout the globe, the need for stringent quality control and standardization becomes paramount to ensure the reliability and reproducibility of outcomes. Good Laboratory Practices (GLP) represent a well-established quality system that provides a framework for the organizational processes and conditions under which non-clinical studies are planned, performed, monitored, recorded, reported, and archived. Originally developed to ensure the integrity of safety data for pharmaceuticals and other regulated products, the fundamental principles of GLP are highly relevant to the field of animal ART. This report aims to explore the application of GLP in ART and to thoroughly investigate its contribution to achieving successful outcomes in various ART procedures and across different animal species. By examining the core principles of GLP and their practical implementation in animal ART laboratories, this analysis will highlight the critical role of standardized practices in enhancing the reliability, ethical conduct, and overall success of animal reproductive technologies.
2. Good Laboratory Practices (GLP) in Reference to ART
Good Laboratory Practices are broadly defined as a quality system that governs the organization and conditions under which non-clinical health and environmental safety studies are planned, performed, monitored, recorded, reported, and archived. This system is designed to ensure the quality and

Zoonoses

Zoonoses are those diseases and infections which are transmitted between vertebrate animals and humans under natural conditions. The emergence of a pathogen into a new host species is called disease invasion or “disease emergence”.

Anthrax is a notifiable disease and primarily occurs in herbivores but it is a type of bio-terrorism agent and called as malignant pustule, malignant edema, woolsorters disease, ragpickers disease, maladi charbon, splenic fever etc. Causative agent is Bacillus anthracis contains vegetative spore and lethal dose is 2,500 to 55,000 spores.

Brucellosis is one of the world’s most widespread zoonoses caused by the genus Brucella. In human it is called Malta fever, Undulant fever, Mediterranean fever, Rock Fever of Gibraltar and Gastric Fever while in animals it is called Bang’s disease, Enzootic abortion, Epizootic abortion, Slinking of calves, Ram epididymitis and Contagious abortion etc. it is also called occupational disease.

Leptospirosis is a disease of animal sand humanbeings caused by genus Leptospira and called as Weil’s syndrome, Canicola fever, Canefield fever, 7-day fever, Rat Catcher’s Yellows, Autumn fever, black jaundice). Fletcher’s EMJH media is used to culture the organism.

Introduction

Protein metabolism is a complex and multifaceted process that plays a crucial role in the overall health and function of living organisms. At the most fundamental level, protein metabolism involves the synthesis, breakdown, and utilization of proteins, which are essential macromolecules that serve a wide range of structural, functional, and regulatory roles within the body.

The process of protein synthesis, or translation, begins with the transcription of genetic information stored in DNA into messenger RNA (mRNA) molecules. These mRNA molecules then serve as templates for the assembly of specific sequences of amino acids into polypeptide chains, which ultimately fold into the three-dimensional structures of functional proteins. This highly regulated process is essential for the production of the diverse array of proteins required for cellular processes, tissue repair, immune function, and numerous other physiological activities. In contrast, protein degradation, or catabolism, refers to the breakdown of proteins into their constituent amino acids. This process is mediated by various proteolytic enzymes, such as endopeptidases, exopeptidases, and dipeptidases, which cleave the peptide bonds that hold the amino acid residues together Protein degradation serves important functions, such as the recycling of amino acids for energy production or the synthesis of new proteins, the removal of damaged or misfolded proteins, and the regulation of cellular signaling pathways.

Antimicrobial Resistance abbreviated as AMR is defined as the process by which microorganisms like bacteria, fungi, viruses, and parasites change over a period and become resistant to a drug to which they were earlier susceptible to. This makes the medicines ineffective for treatment overtime, thus making infections difficult to treat thereby increasing the risk of spread of diseases caused by them and severe illness which may lead to death. This in turn necessitates the need for development of newer antibiotics. However, there has been a sharp decline in the development and manufacture of new antibiotics in the recent years resulting in development of only few antibiotics in the past two decades. The increase in emergence of antibiotic resistance concurrent with decline in development of newer antibiotics poses a serious threat to health of both humans and animals. Every year about 700,000 deaths have been reported to occur due to resistant infections and this rate is likely to increase further with estimated infections due to resistant pathogens expected to reach over 50 million globally by 2050 (O’Neill, 2016). The worrisome trends of increasing antimicrobial resistance across the globe poses a serious threat to both animal and human health as AMR not only increases the morbidity and mortality rates but also increases the economic burden on health care (Zhu et al., 2022). This presents an extremely grave situation as the supply of new antibiotics is inadequate to cope with the increase of AMR pathogens. The unnecessary use of antibiotics globally especially in the livestock sector as growth promoter further helps in selective enrichment of AMR pathogens which further increases health risks. It is well known that resistant pathogenic and commensal bacteria can persist and spread within and between premises despite zero antimicrobial drug use. This may be explained due to maintenance, replication, and further dissemination of resistance genes by horizontal transfer of genes via natural ecosystem and can occur either by conjugation, transformation and transduction; and these mechanisms can rapidly spread AMR genes among microbial community in natural environment and human microbiome, where these resistant bacterial species may be selected due to presence of antimicrobial drugs (Zhu et al., 2022). The physical transfer of bacteria via movement of animals, workers, and equipment and ineffective cleaning and disinfection may also be responsible for increasing the AMR pathogens in the natural environments.

Introduction

According to the body needs, minerals may be divided into 2 groups like macrominerals and microminerals. Macrominerals are required in amounts greater than 100 mg/day. They include 6 elements like calcium, phosphorus, magnesium, sodium, potassium and chloride. Whereas microminerals are also known as trace elements and are required in amounts less than 100 mg/day. They include 10 elements like chromium, cobalt, copper, fluoride, iodine, iron, manganese, molybdenum, selenium, zinc and silicon. Some minerals are always required since small amounts of inorganic salts are always excreted Fruits, vegetables and cereals are the chief sources of the mineral elements in the diet. Milk products supply the majority of the calcium and phosphorus in the diet.

Milk and milk products are the richest source of calcium along with beans, leafy vegetables and egg yolk. It is absorbed from upper small intestine. Absorption of calcium is active process and requires calcium binding protein present in the intestinal mucosal cells. The absorption is regulated by vitamin D, parathyroid hormone, high protein diet, pH, high dietary lactate and citrate are the factors that promotes calcium absorption. Whereas high dietary phosphate, alkalinity and impaired fat absorption are the factors that inhibits calcium absorption. Of the total body calcium, 99 % present in bones and teeth in the form of hydroxyapatite and 1 % is present in body fluids and other tissues. Calcium in bones acts as a reservoir, which helps to stabilize calcium ions in plasma and extracellular f luid Parathyroid hormone and active vitamin D stimulate osteoblasts while estrogen hormone inhibits osteoclasts.

Oligosaccharides comprise 2 to 10 units of monosaccharide molecules, which are released upon hydrolysis. The oligosaccharides are classified depending upon the number of monosaccharide units, e.g., Disaccharides (two monosaccharide units), Trisaccharides (three monosaccharide units), Tetrasaccharides (four monosaccharide units). Disaccharides are the most common oligosaccharides found in nature.
4.1 Disaccharides
Disaccharides are formed when glycosidic linkages covalently bond two monosaccharides together. A glycosidic bond is formed when the hydroxyl group on one of the sugars reacts with the monomeric carbon on the second sugar. A glycosidic bond can be alpha or beta, depending on the steric configuration at carbon one on the monosaccharide unit. During the union of two monosaccharide units, water molecules are eliminated (dehydration reaction or condensation reaction, or dehydration synthesis), and the formation of a glycosidic bond occurs, through which the units are linked via an oxygen bridge.

Introduction

Nucleic acids, primarily DNA and RNA, are essential for the storage and expression of genetic information. Under physiological conditions, the amino and oxo tautomers of purines and pyrimidines predominate. Adenine a., guanine (G), cytosine c., thymine (T), and uracil (U) are the main building blocks of nucleic acids. Other building blocks include 5-methylcytosine, 5-hydroxymethylcytosine, pseudouridine, and N-methylated heterocycles. A -glycosidic bond connects D-ribose or 2-deoxy-D-ribose to the N-1 of a pyrimidine or the N-9 of a purine, with the syn conformers being the most common.

Nucleotides are the monomeric units of nucleic acids that contain a phosphate group attached to the sugar’s 3’ or 5’ hydroxyl. Extra phosphate groups form nucleoside diphosphates and triphosphates. These have high group transfer potential and help make covalent bonds. Cyclic phosphodiesters cAMP and cGMP act as second messengers. 3′ 5′ phosphodiester bonds link nucleotides to form polynucleotides, which have a directional 5′ and 3′ end. Four bases—A, G, C, and T—compose DNA, and hydrogen bonds (A to T, G to C) hold them together to form a double helix. Human DNA consists of approximately 3 billion base pairs organized into 23 chromosomes, encoding around 25,000 protein-coding genes and various non-coding RNAs (ncRNAs).

Introduction

In a historic landmark, Genentech, a young Californian Company pioneering in gene splicing (which includes recDNA technology, genetic engineering, gene transplantation, gene cloning), saw its stock price skyrocket form $35 to a staggering $89 within just 20 minutes of the New York Stock Exchange’s opening bell on October 15, 1980. This was marked as the fastest stock price growth ever witnessed on that exchange. The company’s success was attributed to the ground-breaking work it had been involved in producing human insulin through genetic engineering that offered a revolutionary alternative for diabetics allergic to the then traditional insulin of porcine origin. Genentech’s pioneering work in gene splicing has its roots in the early 1970s. In 1972, Stanley Cohen and Herbert Boyer were credited with creating the first recombinant DNA molecule. Their experiments demonstrated the ability to create recombinant DNA molecules that could be introduced into bacteria like E? coli, offering a foundation for future advancements in genetic engineering.