eChapters

ABSTRACT

Insects are considered the most successful in the animal kingdom. Many factors contribute to their success. One major factor that operates strikingly is the economy and optimal use of energy. One can give several examples taking the life of a single insect

OS COXAE

Ox: The os coxae or hip bone consists of 3 flat bones i.e ilium, ischium and pubis which fuse together to form the acetabulum.

Ilium: is largest of the 3 parts. It shows three surfaces, three borders and three angels. The lateral (gluteal) surface presents gluteal line parallel to cotyloid border. The medial surface consists of rough medial triangular part, the sacral surface for articulation with sacrum and a smooth quadrilateral part, iliac surface. The iliopectineal line separates these surfaces and bears psoas tubercle in its middle (Fig. 6.1).

Peripheral nerves

The peripheral nervous system (PNS) is the part of the nervous system that lies outside the brain and spinal cord. It includes cranial nerves, spinal nerves and their roots and branches, peripheral nerves and neuromuscular junctions. In the peripheral nervous system, bundles of nerve fibres or axons conduct information to and from the central nervous system. The primary role of the peripheral nervous system is to connect the central nervous system to the bones, muscles and skin of the body trunk and the limbs and the organs to allow the complex movements and behaviour.

The peripheral nervous system is divided into the somatic nervous system and the autonomic (visceral) nervous system.

The somatic nervous system is associated with skeletal muscle voluntary control of body movents. It consists of afferent and efferent nerves. Afferent nerves are concerned with relaying sensation from the body to the central nervous system. The efferent nerves are responsible for sending out commands from central nervous system to the body, stimulating muscle contraction. This system is made up of nerves that connect to the skin, sensory organs and all skeletal muscles.

The autonomic nervous system is the part of peripheral nervous system that controls the visceral functions which occur below the level of consciousness. It consists of two divisions, the sympathetic nervous system and the parasympathetic nervous system.

Peritoneal Fluid Peritoneal fluid (PTF) is an ultra-filtrate of plasma. The amount of the fluid and its constituents depends on oncotic and hydrostatic pressures, vascular permeability, mesothelial lining integrity, and lymphatic drainage. In healthy animals, the amount of peritoneal fluid is negligible making its aspiration difficult. However, in large animals, a small volume (up to 5ml) can be aspirated. Volumes of 10ml or above may indicate a pathological process in the abdominal cavity, unless the animal is in late pregnancy. Indications for collection and analysis The collection and analysis of peritoneal fluid is important in the diagnosis of diseases of the peritoneum and the abdominal segment of alimentary tract .Increased accumulation of peritoneal fluid may be evident in congestive heart failure, peritonitis, nephrotic syndrome,

Bougainvillea is relatively pest-free. Worms, aphids, and moths might be an issue. Sometimes it gets attacked by spiders which can be controlled by using miticide and by the fungus Phytophthora which is checked by spraying Blitox (copper oxychloride) (Sharma 1996, Banerji 2010). Although it is normally pest-free plants, but may suffer from worms, snails and aphids. The larvae of some Lepidoptera species also use them as food plants, for example the giant leopard moth (Hypercompe scribonia). Leaf spot disease caused by Cercosporidium bougainvillea has been reported in the U.S.A. (Sobers and Martinez 1966, Sobers and Seymour 1969). When compared to B. spectabilis, B. glabra has been reported to be resistant or only slightly susceptible to the leaf spot disease (Pal and Swarup 1974). Cercosporidium bougainvillea has been reported in Colombo by Pardo-Cardona (1998) and in Indonesia by Kobayashi and Oniki (1994). The disease can be controlled by spraying copper Carbendazim at the rate of 1 g/l at an interval of 10-15 days. Fusarium wilt caused by Fusarium oxysporum in bougainvillea has been reported in Italy (Polizzi et al., 2010). Bacterial leaf spot caused by Pseudomonas andropogonis has been reported in Brunei, Darussalam (Peregrine and Ahmad 1982, Sivapalan and Hamdan 1997) and U.S.A. (Walker 1991, Walker and Hodge 1991). This can be controlled by spraying copper oxychloride at the rate of 3 g/l at intervals of 10-15 days.

Companion animals are all those species of animals which are kept by man for companionship and which are often referred to as pets. It is quite common to see community dogs or cats living in residential colonies. This is because due to very fast urbanization all the open spaces are getting used up for construction of residential complexes, and the animals like dogs and cats living on these open areas are getting displaced. The residents that occupy these residential buildings find these animals to be a nuisance and want them to evicted.

Pet and companion animal can be discussed under street dog/stray dogs and owner dogs.

Introduction

pH is a quantitative measure of the concentration of hydrogen ions in a solution. The pH scale ranges from 0 to 14, with 7 being neutral. Lower pH indicates acidity, while higher pH indicated alkalinity. Pure water at 25°C has a pH of 7.0 as the concentrations of H3 O+ and OH-. pH is defined as potential of hydrogen and is a measure of how acidic or basic a substance or solution is. The concentration of Hydrogen (H+) in a solution determines its pH. Higher concentrations of H+ ions correspond to lower pH values (more acidic), and lower concentrations of H+ ions correspond to higher pH values (More alkaline/basic). pH is an important measurement concept in many fields, including chemistry, biology, and environmental science. pH of blood and other fluids of the body, secretions etc?, determine the health status of an individual. Likewise, pH determines the quality of soil for plant growth. In medical sciences, pH plays a crucial role in maintaining a healthy internal environment for various bodily functions. Internal environment of an animal or human is tightly regulated more so the pH of different body fluids like blood, saliva and stomach acid.

pH balance is a very prime aspect to maintain homeostasis inside a biological system. Speaking of which the first thing that comes to mind is the enzyme function. Many enzymes have a specific pH range for optimal activity. Any deviation from this specific pH range not only significantly disrupt their function but also hinder biochemical processes involved. Most vital operations of a cell are nutrition transport and waste disposal i?e?, excretion. pH of the fluids surrounding cells impacts these activities; hence, maintaining a proper pH is vital for healthy cell function. Different organs of the body need to maintain different pH ranges for functioning accordingly. Stomach operates at acidic pH to digest the food material ingested whereas, blood need alkaline pH for optimal oxygen transport by RBC.

Introduction

The epidemiological risk and hazards to global health security posed by AMR has been reiterating in several World Health Assembly (WHA) declarations and has been prioritized under the Global Health Security Agenda (GHSA). The Indian Ministry of Health & Family Welfare (MoHFW) has also identified AMR as one of the top priorities for collaborative work with WHO and the first National Action Plan on AMR has been implemented (2017-2022). India ranked fifth in antibiotic consumption in food producing animals (ruminants, pigs, and poultry) in 2010. Even though there has been a gradual reduction of 3.6% in antibiotic consumption during 2011-2019 (Koya et al., 2022), with mounting incomes and shifting in dietary patterns in favour of the consumption of animal protein, especially for poultry, the veterinary antibiotic utilization is projected to further rise by 312%, elevating India to the fourth rank by 2030 (Van Boeckel et al., 2015). FAO has already reported some of the peak resistance rates among common commensal and environmental bacteria against antibiotics like penicillin, cephalosporins, erythromycin and co-trimazoles in India (INFAAR). Resistance to the broad spectrum fluoroquinolones and third generation cephalosporin antibiotics is already more than 70% in Escherichia coli, Klebsiella pneumoniae, and, Acinetobacter baumannii and more than 50% in Pseudomonas aeruginosa. Even the microbial resistance to carbapenems, the last-resort antibiotics, is extremely high among gram-negative microorganisms (Sumanth et al., 2016). To overcome these incidences, it is impulsive to understand the microbe and drug interaction in host system.

MOST OF THE ENERGY FOR LIFE COMES FROM SUNLIGHT

Total rate of energy delivery from sun to earth (at the top of the atmosphere) = 175,000 terrawatts

Terrawatt = 10^12 watts

Rate of energy capture by plants from the sun = net primary productivity (NPP) = 100 terrawatts

Only ~ 0.06% of sunlight is captured

Captured in chemical bonds, especially in sugar

Small amounts of energy are available to specialized microorganisms from chemical reactions not linked to sunlight

It is estimated that 99% of the energy used by living cells comes from the sun

Incorporation of sunlight into chemical bonds occurs through the process of photosynthesis

“Invented” by cyanobacteria about 2 billion years ago

Introduction

In the past few decades, persistent change in climatic variables coupled with global warming lead to the genesis of climatic stress. Currently climatic stress is considered as the most serious ultimatum to livestock’s growth, development, production and reproduction in tropics and subtropics including India. Livestock’s in tropical countries are most commonly affected by the menace of climatic stress. It has been shown that, climatic stress upsets animal’s body homeostasis resulting in grievous decline in livestock’s production and productivity across the world. In general, livestock adapt to the climatic insults via behavioural, physiological, biochemical, metabolic, endocrine and molecular responses. Despite of these thermal adaptation mechanisms, livestock’s witnesses the perils of climatic stress thereby incur major loss in their production and productivity. Therefore, in this changing climatic scenario, it is imperative to rigorously understand and explore the precise mechanism of thermal adaptation to generate climate resilient species which could upsurge the socio-economic status of the farmers as well as the country.

Domestic animals including chicken and mammals, belong to the category of homeotherms, as opposed to poikilotherms. Homeotherms or warm blooded animals are those which are able to maintain constant body temperature in the face of varying environmental temperature. The poikilotherms or the cold blooded animals change their body temperature according to the temperature of the surroundings. Most invertebrates and lower vertebrates belong to the category of poikilotherms.

Why do animals have to maintain constant body temperature? As we are aware that enzymatic reactions in the body are temperature sensitive. In fact, a 10 degree centigrade increase in temperature can result in doubling of the reaction rates. In order to maintain optimum functioning of the enzymatic reactions in the body a constant temperature is required. This constant body temperature is not very constant in the sense that it varies somewhat during exercise and also when the animal is exposed to extremes of temperature of the surroundings. That means that the temperature regulation mechanisms in the body are not 100 percent perfect.

At this stage it is desirable to clarify the distinction between surface temperature and core temperature. The surface temperature, in contrast to the core temperature rises and falls with the temperature of the surroundings. It is the temperature of the deep tissues or internal organs which remains constant. The temperature of the rectum is usually taken as a useful indicator of core temperature because of the ease of measurement. The rectal temperature of several domestic animals is given in Table 1.

Domestic animals –including chicken and mammals, belong to the category of homeotherms, as opposed to poikilotherms. Homeotherms or warm blooded animals are those which are able to maintain constant body temperature in the face of varying environmental temperature. The poikilotherms or the cold blooded animals change their body temperature according to the temperature of the surroundings.

Plant hormones - They are defined as:

1. Small
2. Organic compounds;
3. Synthesized by the plant;
4. Active in low concentration (<10^-6);
5. Promote or inhibit growth and developmental responses;
6. Often show a separation of the site of production and the site of action (although this isn’t as clear a distinction as in animals).

Based on these criteria, would the following substances be considered hormones? Ca²⁺, sucrose, 2 4 - D, glycine, or K⁺?

Introduction

Meat has become an integral part of human food to meet the essential nutrients, like proteins. For a balanced diet, a good biological availability of meat is preferred in the modern day era. The per capita consumption of meat in India is only 14g per day as against the actual requirement of 125g. Non availability of quality meat and its exorbitant prices have restricted meat consumption. Increasing the meat production through intensive rearing of various meat animals will help to meet the ever growing demand of a balanced human diet. In the modern era and educational status social taboos are also getting relegated with need of quality proteins.

The very important role of pigs in human life is control of environmental pollution by scavenging the biological waste. Pig converts biological waste material to quality proteins in the form of pork. Pigs have a rapid growth rate, high reproductive efficacy, require comparatively less space, and can be reared on small scale in backyards or as commercial units.

Being polytocous animal, sow give birth several (8-14) piglets in each farrowing, mother pig find several deficiencies and disorders during gestation, farrowing and postnatal conditions. Prior to birth intrauterine competition amongst the feotus piglets for nutrition and gas exchange is a big stress for abortion. Several infectious and non-infectious causes may hamper proper growth and pathological conditions resulting mortality (15-20%) in piglets than weaner and adults (Sarma, et al, 2012). Several risk factors are also responsible for piglet mortality.

Prenatal factors: Insults during pregnancy can impact a piglet’s ability to survive after birth and regulate their body temperature. Some maternal aspects that affect survability and mortality amongst piglets are prolong farrowing duration more than 4 hours, number of parity of sow, usually first and second parity show high mortality than subsequent parities and also older sow lose more piglets.

Introduction

Spectacular accomplishment in every sector has enabled India to be amongtop achieving nations. However, every section of the society is not benefited by these achievements and problem of malnutrition still remains unresolved. Malnutrition is a common problem faced by underdeveloped and developing countries suchas Philippines, Nepal, Sudan,Tanzaniaand Indiasincelongback. Malnutrition includes undernutrition(wasting,stunting,underweight),inadequate vitamins or minerals as well as overweight, obesity and resulting diet-related non communicable diseases(NCDs). As per the latest report of World Health Organization, 52 million children under 5 years of ageare wasted, 17 million are severely wasted and 155 million are stunted, while 41 million are overweight or obese (WHO, 2018). More than 50% women of marriage age are suffering from anemia. If women are malnourished at the time of pregnancyand delivery, the babybornface theproblems such asphysical retardation,sensitive tocommon infections, reduced cognitive abilities, reducedmental development, etc. As per the World Health Organization (WHO) guidelines, starting from the date of conceiving a child, the first 1000 days are a critical window from nutrition point ofview.Unfortunately, inseveral regions of developingcountries includingIndia, neither the mother nor the babyis provided with the right quantity and qualityof food during this window period. Therefore, it is important for the policymakers to identifymalnutrition at an earlystage for timelyplanningand implementation of various intervention strategies at the community, village, and district level. NitiAyog, Government of India (GoI) (previously known as Planning Commission), in an effort to eliminate malnutrition,regularlyplans several short and longtermnutritionalprogrammesand policies.In thischapter,we willdiscuss some of these policies launched since independence and their impact in combatingmalnutrition.

Introduction

Tissue culture refers to the technique of growing cells, tissues, or organs under controlled, artificial conditions. This allows propagation of tissues from a small sample of cells/tissues under sterile conditions. Plant tissue culture is widely used for various purposes, including the production of disease-free plants, rapid multiplication of plants with desirable traits, genetic modification, conservation of rare or endangered species, etc. Tissue culture is a valuable technique because it enables consistent and large-scale production of plants, ensures genetic uniformity, and allows manipulation of plant biology at cellular level. In this chapter, we will discuss about the major types of tissue culture techniques and their advantages, disadvantages, as well as applications in brief.

Different Types of Tissue Culture

Callus culture involves induction and growing of unorganized, undifferentiated cell masses from an explant on a nutrient/culture medium. The part of plant used to induce callus is called explant which can be tissue, organ or part of a plant.

Organ culture is used to maintain or grow an entire organ or a part of organ in vitro. It preserves the structure and function of the organ for studies or transplantation.

Embryo culture involves isolating and growing embryos from seeds or ovules in a nutrient medium. It is used to bypass seed dormancy or rescue embryos from hybrid crosses that may not develop naturally.

Cell suspension culture involves growing dispersed cells or small cell clusters in a liquid medium under continuous shaking. This is used for large-scale production of cells, secondary metabolites, and studying cell biology..

Principle

The plaque assay was developed in order to enumerate and quantify the infectivity of bacteriophages. This technique has been subsequently adapted for application in animal virology and has proven to be a dependable method for quantifying the concentration of various viruses that have been adapted for growth in cell cultures. This assay is designed to quantify the capacity of a solitary infectious virus to create a visible area of cell death on a layer of cultured cells. A plaque forms during the viral infection cycle, in which the host cell dies after viral replication. Some viruses cause cell lysis during replication, while others cause minimal damage to the cells. The plaque assay is a technique used to measure the quantity of viruses that cause the destruction of their host cells, resulting in cytopathic effects (CPE). During a plaque assay, the host cells and virus are briefly co-cultured to enhance the virus's attachment and entry into the host cell. Subsequently, the concoction is introduced into a partially solidified agar medium. The thick agar is poured onto a layer of "bottom agar" which supplies ample nutrients for the host cell. After one round of virus replication, an infected cell will undergo lysis, leading to the release of multiple new viruses. Within the gelatinous substance, these newly released viruses can only infect neighbouring cells. After a subsequent round of replication, these neighbouring cells will undergo lysis. The cells that manage to avoid infection will continue to proliferate. After a period of 24 to 48 hours, plates that were not contaminated with a virus will display a consistent layer of cells. The plates infected with a multitude of viruses exhibit plaques. Quantifying viral titer is essential for assessing viral infectivity. The determination can be made either by conducting a plaque assay or by calculating the infectious dose.

Polysaccharides comprise several monosaccharide units (more than ten) linked by glycosidic bonds; therefore, they are high molecular weight carbohydrates. They are also called polyanhydrides of simple sugars. The most common monosaccharide unit of polysaccharides is D-glucose. The polysaccharides may differ in their monosaccharide units, molecular weight, chain nature, glycosidic bond types (alpha, beta), and linkages (1-2, 1-3, 1-4, or 1-6). They are tasteless, colorless, amorphous powders that are a little water-soluble. Some may form colloidal solutions. The polysaccharides provide structural stability (structural polysaccharides) to cells, prevent cells and tissues from drying out due to their water-binding properties, and reserve food for the future. Reserved polymeric carbohydrates are osmotically less active and can be stored in large quantities within the cell.
The right and left end of polysaccharides, respectively, are called the reducing and non-reducing ends. Based on the functional aspects, they are grouped in two ways.
• The first way depends on its digestibility—1. Nutrient polysaccharides are (digestible) starch, dextrin, and glycogen, and 2. Structural polysaccharides are (indigestible), chitin and cellulose.
• The second way, depending upon the constituent monosaccharide units, they are 1. Homoglycans (same units), and 2. Heteroglycans (different units).

World trade of fresh cut flower is increasing day by day. Fresh flowers lose their freshness and quality both during travel and also during and after arrangements due to flower specific short vase life. Such deficiencies can be ameliorated through application of nutrient additives to vase water. Use of preservative solutions has been known for many years to increase the vase life of cut flowers. Different methods like use of holding, pulsing and bud opening solution, growth regulators, gamma irradiation, pre-cooling, cold storage, packaging etc. are already in practice in the flower trade to ensure the garden fresh quality of flowers to consumers (Singh et al., 2001). The floral business is expanding in a big way, nationwide.  Sales of cut flowers are rising faster through retailers as every state has entered into development of supermarket chains. Cut flower sale through retailers is  a booming business. Retail growers and Wholesale growers are the major players in our country for domestic market. Retail market suffers from logistic bottlenecks. Retailers do not have any background scientific knowledge to increase the vase life of cut flowers. Flower buyers are very choosy and prefer quality bloom with longer vase life.