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Carbohydrates are the most abundant class of organic compounds found in living organisms. Carbohydrates are also called sugars and are found in plants and animals. Plants synthesize them through photosynthesis (endothermic) by fixing carbon, hydrogen, and oxygen in the presence of solar energy and chlorophyll pigments.
nCO2 + nH2O + energy → CnH2nOn + nO2
Carbohydrates are stored as starch in plants as a source of energy. They are also stored in animals as glycogen in muscles and the liver. They are also found in skeletal structures of cell walls in plants (cellulose), bacteria (peptidoglycans), insects, and crustaceans. This stored food is consumed by animals and man, and upon their oxidation in the body, energy is released for various metabolic activities (work, growth, and various productive functions). Compositionwise, plants are more prosperous in carbohydrates (30%) than animals (1%), with water at 60% and ash at 4%. Plants are poor in proteins (5%) and lipids (1%) as compared to proteins (20%) and lipids (15%) in animals.
2.1 Introduction to Carbohydrates
Carbohydrates or saccharides (sakcharon=sugar or sweetness) are a chemical term applied to a large group of substances, which are organic compounds containing elements Carbon, Hydrogen, and Oxygen in a ratio of 1:2:1. Hydrogen and Oxygen atoms are in the ratio of two to one as in water molecules. As the ratio of hydrogen to oxygen is like H2O, they are also known as “hydrates of carbon” and are represented as (CH2O)n. But there is an exception to this general formula. Deoxyribose (C5H1OO4) and rhamnose (C6H12O5) do not have the required ratio of 2:1 for hydrogen to oxygen. On the other side, some compounds possess this required ratio but are not carbohydrates, e.g., formaldehyde (CH2O), acetic acid (C2H4O2), and lactic acid (C3H6O3). Further, some carbohydrates have nitrogen(glucosamine), phosphorus, and sulfur but do not satisfy the above general formula. The simplest sugars are called monosugars or monosaccharides. There are two main functional groups in

The number of hormones secreted by the body exhibits control over the physiological regulation of glucose sugar level (homeostasis) in the manner needed by the body. Depending upon the situation, hormones may increase or decrease the level of glucose sugar present in the blood or tissues. The glucose is oxidized to yield energy, carbon dioxide, and water.
Glucose, more than the body’s needs, is stored or converted mainly to glycogen and, to some extent, protein and fat. If glucose is less than the need of the body, its denovo synthesis takes place from amino acids or fatty acids, or is formed from the breakdown of stored glycogen. Various hormones are secreted by multiple endocrine glands to control the blood glucose level. If it fails, it leads to hypoglycemia or hyperglycemia conditions. Gluconeogenesis is not developed at birth in the case of a baby pig. Therefore, hypoglycemia in baby pigs is observed within 2 days of age if starved, leading to hypoglycemia, weakness, confusion, and death.
15.1 The Hormones Involved in Homeostatic Regulation of Blood
Glucose Level
The hormones influencing carbohydrate metabolism are explained below:

Introduction
Infection prevention and control refers to prevention and control of infections and their transmission in healthcare settings. Healthcare Associated Infections (HAI) are one of the most common adverse events in delivery of care and a major public health problem with an impact on morbidity, mortality and quality of life. Up to 7% of patients in developed and 10% in developing countries acquire at least one HAI. These infections also present a significant economic burden for the health system.1It is evident that HAIs result in prolonged hospital stays, long-term disability, increased resistance of microorganisms to antimicrobials, additional cost on health systems, high cost for patients and their family, and preventable deaths.
The core components of IPC2
• IPC programme at the national and health facility level
• National and facility level evidence-based guidelines on IPC
• Education and training
• HAI surveillance
• Multimodal strategies
• Monitoring and audit of IPC practices and feedback
• Workload, staffing and bed occupancy
• Built environment, materials and equipment for IPC
HAI Burden
HAI are one of the most common adverse events in healthcare delivery and a major public health problem with an impact on morbidity, mortality and

A large proportion of the body of mammals including domestic animals, is made up of water. This water is an essential attribute for the life of the animals. Animals deprived of food but provided water will survive much longer than the animals provided with food but deprived of water. Water is the basis of the liquid environment of the cells, which Claude Bernard called the milieu interieur, and Cannon the fluid matrix.

Enzyme histochemistry constitutes a link between biochemistry and morphology (Hardonk et al., 1976) and provides important information complementary to conventional histology, immunohistochemistry and molecular pathology (Meier Ruge and Bruder 2008). It helps to detect any metabolic changes in tissues and thus can aid in disease diagnosis. It is based on the principal that, the substrates added to tissue are metabolized by enzyme and the reaction is visualized by an insoluble dye product. It is a very sensitive technique that detects even an early metabolic imbalance in the tissue. For example, alkaline phosphatase activity represents tissue barrier functions in brain capillaries, duodenal enterocyte and proximal kidney tubule brush borders and a decrease in enzyme activity will indicate some functional impairment. Similarly, an increase in lysosomal acid phosphatase activity is an early marker of ischemic tissue lesions and acetylcholinesterase enzyme histochemistry has been used for the diagnosis of Hirschsprung disease (Meier Ruge and Bruder, 2008). ATPase methods are used in combination to distinguish between type1 and type 2 muscle fibres and NADH diaphorase is used to detect very minor or early structural abnormality in the sarcoplasmic reticulum network of the fiber, as well as mitochondrial abnormalities eg. Mitochondrial myopathies (Sargaiyan and Bansal, 2014). It has been clearly shown that tumor tissue with high glycolytic activity is particularly resistant to radiotherapy (Mathupala et al., 2007). Enzyme histochemistry for dehydrogenase provides a quick, efficient and inexpensive orientation on the actual energy metabolism of a particular tumor and thus aid in therapeutic strategy (Nakagawa et al., 2002).
For the histoenzymic localization, fresh frozen unfixed tissues preferably 2 to 5 mm square size should be collected immediately after death/slaughtering of animals. Fixation in formalin will inactivate most of enzymes but formalin fixation is also employed to stop enzyme activity of lactic dehydrogenase and

Carnivora is an order of placental mammals that have specialized in primarily eating flesh, whose members are formally referred to as carnivorans. The order Carnivora is the fifth largest order of mammals, comprising at least 279 species. They come in a very large array of different body plans with a wide diversity of shapes and sizes. The common characteristics of carnivores are given below:

• They are meat eaters.

• There is the presence of canine teeth.

• The intestinal tract is short and is adapted to rapid digestion as well as the assimilation of meat.

• All species have anal glands. Some species, like the striped skunk (Mephitis mephitis), may eject the contents of the anal gland as a defensive maneuver.

• Os penis is present in males.

• There is a lack of a clavicle which helps in the freedom of movement of the forelimb.

• Ulna is well developed.

• Toes end in claws.

Introduction

The digestive system and its auxiliary organs main functions include digestion and the absorption of nutrients required for cell metabolic processes. Digestion is the mechanical and chemical or enzymatic breakdown of large nutritional macromolecules into simpler molecules that cells can absorb. It takes place in the aqueous milieu of the digestive system and is secreted by the salivary glands, stomach and mucosal glands, pancreas, and liver.

Digestive enzymes are secreted almost throughout the digestive tract. Salivary enzymes such as lingual lipase and α-amylase initiate the breakdown of fat and carbohydrates in the mouths of young animals (e.g., calves) and omnivores (e.g., rats, pigs, and humans).The optimum pH for oral digestion is 6.7 (6.2-7.6).

The gastric juice contains pepsinogen, HCI, and intrinsic factors. The pepsinogen enzyme is secreted by the stomach’s chief cell. Pepsinogen is converted to pepsin at acidic pH ranges of 2.0 to 3.5 and initiates the digestion of proteins in the stomach of monogastric animals. Also, the stomach’s parietal cells secrete HCI and intrinsic factors. Cyanocobalamin, is a synthetic form of vitamin B₁₂ requires an intrinsic factor in ruminants to be absorbed. The abomasum of young ruminants secretes proteolytic enzymes such as rennin or chymosin. Rennin acts on milk at a pH of 6.5.

Reliable artificial intelligence depends on accurate, timely and multidimensional livestock data. This chapter examines the transition from manual record-keeping to sensor-based systems that capture physiological, behavioural, production and environmental information. Data sources include wearable devices, IoT monitoring networks, computer-vision systems and environmental sensors. Their applications include monitoring health, welfare, movement, feeding and productivity. The chapter also considers the integration of heterogeneous datasets and persistent challenges involving missing values, noise, interoperability, scalability and system reliability. By connecting data generation with intelligent analysis, it emphasizes that robust acquisition protocols and dependable infrastructure are essential for developing effective, evidence-based and responsive livestock-management systems.

Cloud computing and Edge AI provide complementary infrastructure for intelligent livestock management. Cloud platforms offer scalable storage, centralized analytics, model training and integration of data from sensors, cameras, automated feeders and farm-information systems. Edge AI processes information close to the farm, reducing latency, bandwidth consumption and response time. This chapter explains their architectures, workflows and applications in dairy, poultry, swine and small-ruminant production. Uses include disease detection, estrus prediction, behavioural monitoring, feeding and environmental control. Hybrid cloud-edge systems balance centralized intelligence with local autonomy. Challenges involving connectivity, interoperability, cybersecurity, cost, model reliability and ethical animal surveillance are also critically examined.

The muscles of the head (Fig. 2-3 A) are described in four groups viz (1) superficial muscles including the cutaneous muscle. The other muscles in this group of muscles are the muscles of lips, cheeks, nostrils, eyelids and external ear. (2) the orbital muscles (3) the mandibular muscles and (4) the hyoid muscles.

The cutaneous muscle on the head of the ox is well developed and extensive. In most of the regions of the head it is intimately adhered to the skin. The superficial fascia forms a continuous layer beneath the cutaneous muscle in this region except the natural orifices like nostrils, mouth, eyes and the external ear. The deep fascia forms a continuous layer particularly on the temporal, buccal, and the pharyngeal parts of the head. A part of the cutaneous muscle on the frontal region is called the frontalis muscle. The muscle fibres of this muscle blend with the muscles of the eyelids and the contraction of fibres of this muscle causes elevation of the superior eyelid and forms wrinkles to the skin on forehead.

Healthcare organizations require collaborative learning methods that protect sensitive patient information. This chapter introduces federated learning as a framework for training models across decentralized institutions without transferring raw clinical data. Horizontal, vertical and hybrid federated architectures are examined alongside communication protocols, threat models and security requirements. Privacy-enhancing methods—including differential privacy, secure aggregation, cryptographic techniques and trusted execution environments—are discussed. Applications in medical imaging, electronic health-record analysis, wearable devices and remote patient monitoring demonstrate the framework’s practical value. The chapter also evaluates accuracy, privacy, communication cost, scalability and robustness while addressing data heterogeneity, fairness, explainability, legal compliance and ethical challenges in collaborative healthcare analytics.

2.1. Classification of Fish

Fish are incredibly diverse aquatic vertebrates. There are over 32,000 de scribed species of bony fish, over 1,100 species of cartilaginous fish, and over 100 hagfish and lampreys. Fish can be broadly classified into three groups:

2.1.1. Superclass Agnatha (Jawless fish): These are the most primitive fish, lacking jaws and paired fins. They have existed for over 500 million years. Examples include lampreys and hagfish.

2.1.2. Class Chondrichthyes (Cartilaginous fish): These fish have skeletons made of cartilage instead of bone. They have jaws and paired fins. Examples include sharks, skates, and rays.

2.1.3. Superclass Osteichthyes (Bony fish): This is the largest and most diverse group of fish. They have skeletons made of bone and have jaws and paired fins. Examples include tuna, salmon, goldfish, and many more.

Introduction

Billions of chemical reactions are happening inside human body and that too with lightning speed. However, the same reactions if carried out in laboratory, they take a very long time. The reasons behind it are the biocatalysts, also known as enzymes, which are synthesized by living cells. Chemically, they are proteins(except ribonucleic acids: RNA), colloidal in nature, thermo-labile in character and very specific in their action. For example, hydrolysis of proteins by a strong acid at 100°C takes a couple of days; but same proteins can be digested within couple of hours inside human body at 37°C. Similarly, carbonic anhydrase is used as a catalyst by living cells to transfer carbon-di-oxide from cells to blood. This process is 107 times quicker than the same process if occurs without the help of enzymes. Therefore, enzymes can be defined as the biomolecules or biocatalysts which can speed up the rate of the chemical reactions. The substance upon which an enzyme acts is called as substrate and the one which is produced as the result of enzyme-substrate reaction is known as product.

Introduction
From the tiniest insects to the largest blue whales, all species in the animal kingdom have been shown to undergo the migration. Millions of animals embark on epic journeys every year in search of food, shelter, and opportunity for mating. These animals routinely travel thousands of kilometres by land, sea, or air, which both tests and improves their endurance. Among the most striking animal migrations are those of Arctic terns. The long-distance migration of these little birds from pole to pole is well-known. They are thought to move over 40,000 kilometres (25,000 miles) a year, spending the majority of their time at sea chasing an endless summer. One of the most well-known migrations is that of the monarch butterfly, which travels across several generations. Though they are widespread throughout the United States and beyond, monarch butterflies are most known for their ability to travel 4,800 kilometres (3,000 miles) from Canada to Mexico. Millions of monarch butterflies fly south each year from their northern ranges to the oyamel fir forests near the Sierra Madre mountains, where they build massive roosts to remain warm throughout the winter. The monarchs begin their annual migration northwards in the spring, taking three to five generations to reach their goal. The migration that is the most dramatic belongs to the wildebeest. They coexist with thousands of zebras and gazelles in enormous herds that number over a million. The yearly migration of wildebeest across Tanzania and Kenya in quest of new water and grazing land is well-known. One of the most magnificent displays of nature is thought to be the migration. Animals that migrate are essential to preserving ecosystem balance and sustaining life as we know it. They distribute seeds and serve as pollinators, which support the structure and functionality of ecosystems.

Introduction

Rainfed agro-ecosystems occupy a considerable place in Indian agriculture, covering 80 M ha in arid, semi-arid and sub-humid climatic zones; constituting nearly 57% of the net cultivated area. Rainfed areas contribute almost 100% of forest products, 84-87% of coarse-grain cereals and pulses, 80% of horticulture, 77% of oilseeds, 60% of cotton and 50% of fine cereals including rice, wheat, maize, sorghum etc (Srinivasarao et al., 2010; 2011). Rainfed regions support 60% of livestock, 40% of human population and contribute 40% of food grains and several special-attribute commodities such as seed spices, dyes, herbs, gums, etc. (Srinivasarao et al., 2011). It is estimated that even after achieving the full irrigation potential, barring for successful completion of river linking project, nearly 45 to 50% of the total cultivated area will remain dependent on rain. Undoubtedly, the rainfed agriculture would continue to occupy a prominent place in Indian agriculture for a long time to come (Venkateswarlu et al., 2011). Despite the progress made so far, rainfed agriculture in India encounters multiple risks and constraints relating to biophysical and socio-economic issues. Agricultural productivity in rainfed areas continues to remain low and unstable due to weather aberrations, nutrient disorder and poor socio-economic status of farmers.

In recent past, extreme weather events are causing great concern in different sectors contributing to Indian economy. India Meteorological Department is doing yeomen’s service by providing advance information including monitoring of the extreme events longs with proper advisories to the farming community by using state of art instruments & technology through efficient delivering mechanism of the information and ultimately help the farmers from incurring great loss. In many cases, the most severe impacts are felt when several extreme events occur together. The major extreme weather events related to agriculture are Tropical cyclones, floods, heavy rain and landslides, cold waves, fog, snow storms, Heat waves, Hailstorms, thunderstorms and dust storms, drought.

Healthcare artificial intelligence depends on the quality, fairness and reliability of the data used for model development. This chapter examines the characteristics of healthcare data and explains how incomplete, inconsistent or unrepresentative datasets can produce inaccurate and discriminatory clinical outcomes. It identifies sampling, demographic, measurement, historical, institutional and annotation biases and discusses strategies for mitigating them at the data, algorithm and evaluation stages. Model robustness across different populations and clinical environments is also considered. Particular attention is given to explainable AI, human oversight, continuous auditing and interdisciplinary collaboration as essential components of trustworthy, equitable and clinically dependable healthcare systems.

1    General post mortem examination and detection of date and time of death

2    Pathogenomonic lesions of different diseases

3    Livestock insurance

4    Pet-dog insurance

Increasing evidence over the past few decades indicate that significant changes in climate are taking place worldwide as a result of enhanced human activities. The inventions that were discovered during last few centuries, more so in the last century has altered the concentration of atmospheric constituents that lead to global warming. The major cause to climate change has been ascribed to the increased levels of greenhouse gases like carbon dioxide (CO₂), methane (CH₄), nitrous oxides (NO₂), chlorofluorocarbons (CFCs) beyond their natural levels due to the uncontrolled activities such as burning of fossil fuels, increased use of refrigerants, and enhanced agricultural related practices. The temperature increase is widespread over the globe and is greater at higher northern latitudes. Land regions have warmed faster than the oceans. January 2000 to December 2009 was the warmest decade on recordreported by NASA. These activities accelerated the processes of climate change and increased the average global temperatures by about 0.8°C (1.5°F) since 1880 (NASA,2010). Global average sea level has risen since 1961 at an average rate of 1.8 [1.3 to 2.3] mm/yr and since 1993 at 3.1 [2.4 to 3.8] mm/yr, with contributions from thermal expansion, melting glaciers and ice caps, and the polar ice sheets. Satellite data since 1978 show that annual average Arctic sea ice extent has shrunk by 2.7 % [2.1 to 3.3%] per decade, with larger decreases in summer of 7.4% [5.0 to 9.8%] per decade. Mountain glaciers and snow cover on average have declined in both hemispheres. It has also induced increased climatic variability and occurrence of extreme weather events in many parts of the world.

Hausman (1920a) worked on the microscopic structure of hair from the commercial fur. The hair samples of 27 different species of animals were used for the study. He described in detail the structural features of guard hair. He classified medulla of hair into 4 different types as discontinuous, continuous, fragmental and interrupted on the basis of composition of medullar cells. He also classified the cuticular scale pattern into two types as imbricate interrupted and imbricate coronal. He mentioned in detail the methods for the preparation of slides for the study of microscopic structures of hair. He also studied the cortex and pigment granules.