eChapters

11.1 Total DNA Extraction by cTAB Method
    
11.2 Extraction, Purification Estimation of DNA
    
11.3 Potassium Acetate Procedure
    
11.4 DNA  Estimation
    
11.5 Restriction Enzymes

1    Large animals

2    Canines

3    Suggested antimicrobial/antifungal agents for intra-uterine administration

4    Commercial preparations of hormone(s) and recommended dosage protocol in cows

5    Drugs safe & contra indicated in pregnancy

The  information on normal reproductive traits in domestic animals like calving interval, age at first calving etc, is essential for getting the maximal reproductive efficiency of the animal by the farmer. It also helps in improving the reproductive efficiency of the animals by different required means viz nutrient/mineral supplementation, antibiotic, hormonal therapy etc.

Climate change is a statistical variation in properties of the climate system that includes changes in global temperature, precipitation, etc due to natural or human drivers over a long period of time. It may affect distribution and quality of natural resources thereby influencing livelihood security of the people (Status of Indian Agriculture, 2013). Recent climate change activities is the increase in earth’s temperature by 0.74^ºC between 1906 to 2005 (IPCC 2007) due to increase in anthropogenic emissions of green house gases such as carbon dioxide (CO₂), methane (CH₄), ozone (O₃), nitrous oxide (N₂O) and chlorofluorocarbon (CFCs). Increase in concentration of green house gases influence future climate as well as agriculture (Mall et al., 2006; IPCC, 2001; Aggarwal, 2003: Bhatia et al., 2004). For Indian region under South Asia, Intergovernmental Panel on Climate Change (IPCC), 2007 projected a rise in temperature from 0.5-1.2^ºC by 2020, 0.88-3.16 oC by 2050, and 1.56 - 5.44^ºC by 2080. This may anticipate greater instability in agricultural production (Aggarwal, 2008). Beside this, climate change also affects the dynamics and frequency of extreme events like tropical cyclones, associated storm surges, and rainfall events. Low lying regions are more vulnerable to climate change as increase in temperature melt down the glaciers causing floods, submergence, and degradation of these regions. Therefore, there is a need for a ‘wake up call’ at this stage to aware farmers about the climate change, extreme weather events like hailstorm, heat and cold waves, drought, flash and river floods and landslides, and its affect on crop and agriculture, and strategies to overcome it.

Vaccines
    
Veterinary v/s Human Vaccines
    
Modern Veterinary Vaccines
    
rDNA Technology to Prepare Vaccines
    
Veterinary Viral Vaccines
    
Live Vaccines
    
Killed Vaccines
    
Genetically Engineered Viral Vaccines
    
Veterinary Bacterial Diseases Vaccines
    
Veterinary Parasitic Vaccines
    
Vaccines against cancer
    
Swine Flu
    
Foot and Mouth Disease
    
Adenovirus Vector Vaccine
    
Johne’s Disease
    
Diseases of Poultry
    
Newcastle Disease in Poultry
    
Avian Influenza (Bird Flu)
    
Rabies
    
Disease Diagnostics
    
The Nucleic Acid-hybridization Technique
    
Diagnostics Based on pcr
    
Diagnosis by Restriction Fragment Length Polymorphisms and Related DNA-based Approaches
    
Diagnosis by DNA Probes and DNA Microarray Technology
    
Antigen-Capture Enzyme-Linked Immunosorbent Assay (ELISA) Proteomics
    
Disease Diagnosis Kits Developed
    
Brucelosis
    
Rabies
    
Canine Distemper and Canine Parvo Virus
    
Canine Hepatitis Virus
    
Foot and Mouth Disease
    
New Castle Disease
    
Egg Drop Syndrome Virus-76 (EDS-76)
    
Proteomics for Disease Diagnostics
    
Nanotechnologies in Diagnosis and Vaccine Development
    
Monoclonal Antibodies
    
Immunotherapy
    
Problems
    
Animal Genomics for Disease Resistance
    
Gene Knockout Technology
    
Gene Pharming
    
Gene Therapy
    
Types of Gene Therapy
    
Germ Line Gene Therapy
    
Somatic Gene Therapy

Introduction

Agroforestry offers both adaptation and mitigation options to climate change especially for the poor rural communities in the developing world. It is now getting ample attention from different investors, scientists, policy makers and international bodies due to its multiple benefits to the environment; and remain an important component of sustainable land use in agricultural production. Agroforestry provides economic, environmental, and social security to the vulnerable communities who are dependent on agricultural production for their livelihoods, and on forest/ trees for their energy requirement. Agricultual production is vulnerable to climate change, and there is a need to cushion these communities to be less vulnerable to climate change, or natural hazards. The suitable, sustainable and smart method is agroforestry based agriculture which offer host of ecosystem services to cope the vagaries of climate change, particularly in rural areas.

1    Ideal surgical pack

2    Nerve blocks

3    Commonly used pre-anaesthetics and anaesthetics

r DNA Biotechnology
    
Concept of r DNA
    
DNA Preparation from Tissue
    
Reagents
    
Preparation
    
DNA Buffer (Tris-EDTA)
    
RNase A (20 mg/ml)
    
Toe DNA Preparation for Southern Blots
    
Southern Blot
    
PCR
    
Polymerase Chain Reaction
    
The Cycling Reactions
    
Denaturation at 94°C
    
Annealing at 54°C
    
Extension at 72°C
    
DNA Sequencing
    
The Sequencing Reaction
    
Denaturation at 94°C
    
Annealing at 50°C
    
Extension at 60°C
    
Separation of the Molecules
    
Detection on An Automated Sequencer
    
Assembling of The Sequenced Parts of a Gene
    
Freezing of Buffalo Semen (Jindal, 1995)
    
Tissue Culture
    
Preparation of Media
    
Laboratory Procedure for IVF (Kharche et al., 2008)
    
Source of Oocytes
    
Preparation of Estrous Goat Serum
    
Collection of Oocytes
    
Laparoscopic Ovum Pick-up
    
Oocyte Retrieval from Ovaries Collected form Slaughter 
    
House
    
Slicing Technique
    
Follicle Puncture Technique
    
Follicle Aspiration Technique
    
Follicle Isolation Technique
    
Evaluation of Oocytes
    
In vitro Maturation of Oocytes
    
Preparation of Cells for Co-culture
    
Assessment of Nuclear Maturation After in vitro Maturation of Oocytes
    
Analysis of Nuclear Status is Ascribed to One of the Following
    
Sperm Preparation for in vitro Fertilization of Oocytes
    
In vitro Fertilization of Oocytes
    
Assessment of in vitro Fertilization of Oocytes
    
Assessment of Cleavage
    
In vitro embryo culture

7.1 Gel Electrophoresis
    
7.2 Polymerase Chain Reaction (PCR)
    
7.3 Restriction Fragment Length Polymorphisms (RFLPs)
    
7.4 Random Amplification of Polymorphic DNA (RAPD)
    
7.5 Amplified Restriction Fragment Length Polymorphism (AFLP)

2.1     Regulation of the Cell Cycle 
    
2.2     Mitosis
    
2.3     Meiosis

1.1     Prokaryotic 
    
1.2     Eukaryotic
    
1.3     Cell orgenellie

The camel is an important animal component of the fragile desert eco-system. With its unique bio-physiological characteristics, the camel has become an icon of adaptation to challenging ways of living in arid and semi-arid regions. The proverbial Ship of Desert earned its epithet on account of its indispensability as a mode of transportation and draught power in desert but the utilities are many and are subject to continuous social and economic changes. The camel has played a significant role in civil law and order, defence and battles from the ancient times till date. The world famous Ganga-Risala of erstwhile Bikaner State was accepted as Imperial Service Troup and participated in World War I and II.

10.1 Plasmids
    
10.2 Retrovirus

The UN designation of 2013 as the International Year of Water Cooperation aptly demonstrates growing gap between water supply and demand. These symptoms, which are already visible in a few regions around India, are soon to assume a national proportion and may become permanent feature of the water sector in the country, unless suitable policies are adopted quickly to manage water demand and supply at different levels. Water demand is growing fast due to a rapid population growth and economic activity, but water supply is not growing at the same rate because of serious ûnancial and physical limits for supply augmentation. Water resources developed at present amount to about 680 km³, which constitutes 61% of the utilizable potential of 1122 km³. However, it is difficult to add supply beyond this level due to heavy costs, environmental concerns and interstate water conûicts. In contrast, the total demand for water is projected to reach 784–843 km3 by 2025 and 973–1180 km³ by 2050 (Ministry of Water Resources, 2000). Recent research studies predict that, if the demand– supply gap continues to increase, nine basins that have over four-ûfths of the total water use in India will face physical water scarcity by 2050 (see Amarasinghe et al., 2007). For a heavily populated, monsoon-dependent and rural-based country, such as India, water scarcity of this magnitude will not only lead to serious water conûicts among sectors and regions, but also have a devastating effect on the food and livelihood fronts.

India has shown to the world that with 2.4 % of the land and 4 % of the water resources available at the global level, it can provide the food security to its millions. A record production of almost 265 million tonnes in 2013-14 is a testimony to the strides taken in the field of agriculture. However, the agriculture sector faces many new challenges in the future and climate change (global warming) is perhaps the most formidable one.

The new emerging demands of the relatively more-affluent Indian population, particularly its middle class, coupled with a net cultivated area unlikely to exceed 143 Mha in 2050 as well as an estimated rainfed agriculture to cover around 45 % of the net sown area, are further compounded with the harsh reality that highly productive agricultural land is being continuously lost out to the industry and urban sectors. How will the country meet the target of 400 Mt of food grain production is the mute question, in a situation where natural resource base is continuously degrading and climate change with its attendant impact is adversely affecting the agricultural production system (Singh, 2014).

Introduction

In many developing countries including India, agricultural sector is of vital importance to the economy and welfare of the inhabitants. Agriculture contributes to 14% of the Indian GDP and supports the livelihood of two-thirds of the population. Despite tremendous technological developments, vagaries of weather continue to plague the agriculture production. Any shortfall in agricultural production will have its cascading effect on the economy of the country. In recent times, climate change and variability are confounding the troubles already faced by Indian agriculture and the farming community. Loss in crop production and farm income due to unexpected weather hazards is beyond the carrying capacity of resource poor farmers of the country. With the growing commercialization of agriculture, the magnitude of crop loss due to unfavourable weather hazards is increasing leading to suicides of farmers. The State and National governments on their part are coming to rescue of farming community by implementing various relief measures like reduction or suspension of land revenue taxes, loan waiving, relief from calamity relief fund etc. Though these measures are helpful, farmers cannot expect them as a right and they put lot of burden on the country’s exchequer.

Introduction

Adoption of green revolution technologies during 1960s led to increased productivity and elimination of acute foodgrain shortages in India. These technologies primarily involved growing of high-yielding dwarf varieties of rice and wheat, increased use of chemical fertilizers and other agrochemicals, and spread of irrigation facilities. This was also accompanied by other modern methods of cultivation, which included maximum tilling of land, virtually clean cultivation with complete removal of crop residues and other biomass from the field, fixed crop rotations mostly involving cereals, and elimination of fertility- restoring pulses and oilseed crops in the highly productive north-western plain zone of the country.

Punjab, known as the “breadbasket of India,” relies heavily on livestock, particularly cattle and buffalo, for its agricultural prosperity. These animals play a crucial role in the region’s dairy industry, contributing significantly to milk production and rural livelihoods. However, like all livestock, cattle and buffalo in Punjab are susceptible to a range of diseases that can impact their health, productivity, and overall well-being. Timely and accurate diagnosis is essential for effective treatment and prevention. This article explores some of the most common diseases affecting cattle and buffalo in Punjab, along with the diagnostic procedures used to detect and manage these health issues, ensuring the sustainability and growth of the dairy sector.

Hydatidosis

Hydatidosis is a zoonotic disease caused by metacestode stages of parasite Echinococcus granulosus and is recognized as a major public health problem worldwide. This tapeworm is found in small intestine of the canid (definitive host) where it produce proglottids containing eggs. Upon shedding through faeces the later are ingested by the cattle, buffaloes and other intermediate hosts including man. These hatch in their intestine and the oncosphere invade circulatory system and lodge in various organs (liver, lung, brain etc.) where they develop into large thick walled unilocular cavity called hydatid cyst, which expands and can reach very large size and buds hundreds and thousands of protoscoleces endogenously. In a survey on hydatid cysts, a prevalence rate of around 5% has been recorded in more than 4000 meat samples of different animals in Punjab.

Introduction

Canine epilepsy, a prevalent neurological disorder, significantly impacts the quality of life for both dogs and their owners. Traditional anticonvulsant therapies often yield inconsistent outcomes, necessitating the exploration of alternative treatments such as homeopathy [1][2]. Homeopathy’s individualized approach aligns with the principles of unicism, focusing on holistic care tailored to each patient’s unique symptom profile [3]. However, the subjective nature of remedy selection and response assessment poses challenges for practitioners.

The integration of Artificial Intelligence (AI) and Machine Learning (ML) offers a promising avenue to address these challenges [4]. Predictive models can analyze complex datasets to uncover patterns that guide remedy selection and treatment strategies. This study evaluates the comparative performance of Logistic Regression, Random Forest, and XGBoost in predicting treatment outcomes for canine epilepsy managed with homeopathic remedies.

Accidental sampling: Accidental sampling as quota sampling is based upon the convenience in accessing sampling population. While quota sampling attempts comprise people possessing an obvious/visible characteristic, accidental sampling makes no such effort. Any person that comes across can be contacted for participation in the study. Collecting data are stopped when reaching the required number of respondents decided to have in the sample.