eChapters

“It  is not uncommon for animals to exhibit behavioral changes before an impending disaster. Wild animals often vacate areas, gather in strange groupings and sometimes will even enter into human habitats they normally avoid,” animal behavior expert Diana L.Guerrero said.Three days before the earthquake, thousands of toads roamed the streets of Mianzhu, a hard-hit city where at least 2,000 people have been reported killed.

Animal behaviour provides valuable information about health, welfare, reproduction, environmental adaptation and social interaction. Traditional observation is labour-intensive, time-consuming and vulnerable to observer bias. This chapter explains how machine learning automates behavioural analysis using video, sensor and bioacoustic data. It covers preprocessing, feature extraction, classification, clustering and recurrent neural networks for temporal analysis. Computer-vision techniques—including object detection, tracking and pose estimation—enable continuous and non-invasive monitoring. Case studies demonstrate applications in livestock management, wildlife conservation and laboratory research. The chapter also examines data labelling, model generalization, ethics, explainable AI, real-time processing and the integration of multiple behavioural data sources

Introduction
The behaviour of animals during restraining, handling, and feeding is a crucial aspect of animal husbandry and veterinary care. Understanding and interpreting their behaviour in these contexts is essential for ensuring the well-being of animals and the safety of humans involved in their care.
I. Animal Behaviour During Restraining
1. Fear and Anxiety: When animals are restrained, they often exhibit fear and anxiety. This behaviour can manifest as vocalizations, attempts to escape, increased heart rate, and sometimes aggressive actions. Understanding the signs of fear and anxiety in animals is essential to mitigate stress during restraint. Calm and gentle handling can help reduce the negative impact on the animal’s well-being.
2. Fight or Flight Response Animals: When restrained, may respond with a “fight or flight” reaction. They may try to flee or become defensive, potentially causing harm to themselves or the handlers. It is essential to anticipate this response and use appropriate restraints, such as halters or cages, to ensure safety for both animals and handlers.
II. Animal Behaviour During Handling
1. Trust and Bonding: The behaviour of animals during handling can vary depending on their previous experiences and the quality of their relationship with their caregivers. Animals that trust their handlers and have positive associations with handling are more likely to exhibit calm and cooperative behaviour. Trust is built through consistent, gentle, and respectful handling over time.
2. Aggression and Fear: Animals that have had negative handling experiences may exhibit aggression, avoidance, or fear during handling. This can pose risks to both the animals and the handlers. In such cases, it is essential to employ behaviour modification techniques and gradually desensitize the animals to handling procedures.

Animal Biotechnology

Biotechnology is defined as “the application of biological organisms, systems or processes to manufacturing and service industries” (Coombs, 1984).

It is also defined as “the application of scientific and engineering principles to the processing of materials by biological agents to produce goods and services” (Coleman, 1986).

Animalbiotechnologyis the application of scientific and engineeringprinciples to the processing or production of materials by animals to provide goods and services to human kind.

14.1 NEED FOR ANIMAL DISEASE INFORMATION SYSTEMS

Once the animal disease data are gathered, a few things need to be done with the data so collected. First, they must be managed and quality checked; Secondly, they must be analyzed to be understandable; and thirdly, they must be acted upon. For data to be analyzable and useful for decision-making, they need cautious management and quality control. An information system is the collection of data, people, procedures, hardware, software, files, and data required to accomplish an organized set of functions.

The constituents of an information system are:

• People – those who gather data, those responsible for data input and those responsible for data analysis

• A storage / retrieval / analysis system – which consists of computer hardware and appropriate software

• A feedback delivery system – mechanisms to ensure that processed data are fed back to data gatherers

History of Genetics

Lamarck (1744-1829): Proposed theory of “Inheritance of acquired characters”.

Charles Robert Darwin (1809-1882): published his classic book “Origin of Species” in 1859 and proposed theory of evolution and survival of the fittest.

Johann Greger Mendel : Gave laws of heredity in 1866 and is considered as Father of Genetics.

W. Flemming: The term mitosis was first used by Fleming in 1882.

W. Waldeyer: Coined the term “Chromosome” in 1888.

August Weisman: Gave the “Theory of Germplasm” in 1889.

Farming System of India

Based on how income is derived from a farming enterprise: Specialized farming, diversified farming and mixed farming.

Based on mode of ownership and organization of farms: Individually owned, co-operative farming and collective farming

Based on scale of operations: Small scale farming and Large scale farming.

Introduction

Advancement in agricultural research has increased productivity however, in recent years, poor linkage of research - extension services hampering the growth of agriculture sector. Firstly, the research problems are investigated without considering the priority of agricultural producers Secondly, knowledge generated at the agriculture research stations is not effectively transferred to the producers. Many studies have identified the weaknesses in the linkages between the research and extension institutions are the major reason for development of technology and its transfer. In most countries including our country, the communication between research and extension agencies is weak (USAID 1982, FAO 1984). Research-extension-farmer linkages are seen to be absent or weak in many instances. At the same time, there are duplications of efforts, with a multiplicity of agents attending to extension work without adequate coordination (Planning Commission, 2008). This ineffective linkage between research and extension has impeded the generation and transfer of technology appropriate for small-scale and resource poor farmers, particularly those who work in relatively low productive and heterogeneous agro-ecological areas (Ewell 1989). Problems in technology generation and transfer arise because these components are considered in isolation (World Bank 1985).

Partitioning of Energy in Livestock

Energy Partitioning

Partitioning of energy involves loss and distribution of feed energy into different body functions.

Gross energy is calculated from the heat produced when a sample of feed or feces is subjected to complete combustion in a bomb calorimeter under a pressurized oxygen atmosphere.

Apparent digestible energy is the difference between the gross energy of the feed consumed and the gross energy of the feces excreted. Digestion is accompanied by the production of gases in both fore- and hindgut and by the urinary excretion of metabolites.

Metabolizable energy is the difference between digestible energy and energy lost to gases and urine. Metabolic processes and physical activity produce heat (heat of production) which may warm the animal in cold weather. In hot weather, energy may be required to expel excess heat of production, through panting and increased respiratory rate.

Net energy is the remaining energy available for maintenance, reproduction and production. The diagram on the left illustrates energy partitioning for a particular cow near the end of her lactation. Energetic efficiency of this cow is about 29%, expressed as output in the form of milk, gain and fetal growth divided by gross energy input. Although their energy requirements are significantly greater, higher producing cows are also more energetically efficient.

Veterinary oncology is an upcoming field in recent years due to the integration of the importance of spontaneous canine cancers in understanding and developing novel strategies for human cancer management. We are stepping towards a very exciting era in veterinary oncology, where comparative oncology has played a significant role for many decades in understanding the molecular basis of cancer in companion animals. The comparative animal models have generated remarkable interest in both academia and industry. Studies have demonstrated the utility of comparative oncology in understanding drug resistance and tumor biology, contributing to the translational research[1]. This has yielded breakthrough discoveries, from advanced diagnostics to novel therapies, providing opportunities to improve the longevity and quality of life of the pets. These inputs have led to advancements such as the first conditionally approved chemotherapeutic agent for lymphoma, novel BRAF testing for canine urothelial carcinoma, tyrosine kinase inhibitors for mast cell tumors, updates in stereotactic/FLASH radiation, and novel immunotherapeutics. As the understanding of cancer biology has advanced, the field of veterinary oncology has grown significantly, paralleling developments in human oncology. Veterinary oncologists treat a variety of cancers in domestic animals, particularly dogs and cats, but also in exotic animals, horses, and even zoo animals. While the types of cancers in animals share similarities with human cancers, treatment strategies can differ significantly due to species-specific differences in anatomy, physiology, and response to therapies [2, 3].

1 History

Animals are culturally worshipped and revered as gods in India by different communities such as cow as Kamadhenu by Hindu religion. The scientific study of animal welfare has rapidly grown during last few years. Many religions follow principles of ahimsa such as Jains and Hindus do not harm cattle; Muslims do not harm pigs etc. Thus caring for welfare of animals is an inborn attitude of majority of Indians. As per the Indian tradition and culture, animals always had a respect and a special place in society. Hinduism, Buddhism and Jainism have always preached kindness and compassion to animals. Each Hindu God or Goddess is seen with an associated animal as vehicle or incarnation. For example, Lord Krishna was a shepherd and is seen with a cow, Lord Rama with the monkeys, Lord Vishnu with the eagle and Lord Shiva with a snake around his neck and the bull ‘Nandi’ at his feet, Goddess Saraswati is the goddess of wisdom and literacy is seen with swan.

Animal Welfare Board of India was started under the stewardship of Late Smt. Rukmini Devi Arundale, well known humanitarian. The Animal Welfare Board of India is a statutory advisory body on Animal Welfare Laws and promotes animal welfare in the country. The board established in 1962 under Section 4 to 10 of the Prevention of Cruelty to Animals Act, 1960 (No. 59 of 1960).  The primary sources of funding of the AWBI are grants from the Government of India. However, contributions, subscriptions, bequests and gifts will also be encouraged.

A disaster (from Latin meaning, “bad star’) is the impact of a natural or man-made event that negatively affects life, property, livelihood or industry often resulting in permanent changes to human societies, ecosystems and environment. The event itself is not a disaster; it is the impact that is a disaster. Their possibility of occurrence, time, place and severity of the strike can be reasonably and in some cases accurately predicted by technological and scientific advances. Hence, we can to some extent reduce the impact of damage though we cannot reduce the extent of damage itself.  The impacts suffered include trauma, starvation, dehydration, infection, disease, shock etc. Animals are often the silent victims of disasters, and there is a real need around for the provision of effective training and management programmes to ensure that their survival and welfare are adequately accounted for. This demands the study of disaster management in methodical and orderly approach.

Animal welfare organizations are those organizations which are volunteered to help people to protect their animals. It is concerned with the health, safety and psychological wellness of animals. They have a deep understanding of needs of animals and thus help animals in distress. The types of stake holders depend on what type of animals are reared.

Introduction
The discovery and widespread use of antibiotics in the mid-20th century marked a transformative era in modern medicine, enabling life-saving interventions in surgery, transplantation, and critical care. Diseases once considered fatal became manageable, and the global burden of infectious diseases significantly declined. However, the remarkable success of antibiotics has been increasingly overshadowed by the rapid rise of antimicrobial resistance (AMR), now recognized by the World Health Organization (WHO) as one of the most serious threats to global health, food security, and sustainable development. Resistant infections already contribute to longer hospital stays, increased healthcare costs, and elevated mortality rates. Alarmingly, if current trends continue, AMR-related deaths are projected to exceed those caused by cancer by 2050.A pivotal yet often underestimated factor in the persistence and spread of AMR is the formation of microbial biofilms. Biofilms are highly organized, surface-attached communities of microorganisms embedded in a self-produced extracellular polymeric substance (EPS) composed of proteins, polysaccharides, lipids, and nucleic acids. This matrix not only anchors microbial population but also confers protection against environmental stressors, immune responses, and antimicrobial agents. Bacteria within biofilms can withstand antibiotic concentrations up to 1000 times higher than their planktonic counterparts due to altered metabolic states, enhanced horizontal gene transfer, and activation of resistance mechanisms such as efflux pumps. Biofilms are implicated in over 80% chronic and device-associated infections, including those caused by ESKAPE pathogensAcinetobacter baumannii, Pseudomonas aeruginosa, Klebsiella pneumoniae, and Staphylococcus aureus. These infections are notoriously difficult to treat and often recur, posing significant clinical challenges. Beyond healthcare settings, biofilm formation in livestock and agricultural environmentsexacerbated using antibiotics as growth promoterscontributes to the emergence of resistant strains that can spread to humans via direct contact, environmental contamination, or the food chain.Understanding the multifaceted resistance mechanisms within biofilms is essential for developing effective containment strategies. These include physical exclusion of antibiotics by the EPS matrix, metabolic dormancy, persister cell formation, quorum sensing-mediated coordination, and stress response activation. Moreover, biofilms often consist of multispecies communities, where interspecies interactions further enhance resilience.In response, antibiofilm agents have emerged as a promising frontier in AMR containment. These include natural compounds (e.g., antimicrobial peptides, essential oils), engineered materials (e.g., nanoparticles, surface coatings), quorum sensing inhibitors, efflux pump blockers, and enzymatic disruptors such as dispersin B and DNases. Ecological approaches like probiotics and positive biofilms offer preventive strategies in agriculture and food systems. Importantly, these agents aim to complement antibiotics by enhancing their efficacy rather than replacing them.Despite challenges in scalability, safety, and regulatory approval, antibiofilm strategies represent a paradigm shift in infection control. Their integration into clinical, veterinary, and environmental practicesaligned with the One Health frameworkis essential for preserving antibiotic effectiveness and mitigating the global AMR crisis.

Introduction
The discovery of antibiotics in the early 20th century revolutionized medicine, agriculture, and public health. These compounds, capable of inhibiting or destroying pathogenic bacteria, not only saved millions of human lives but also transformed animal production systems by reducing morbidity and mortality, improving growth rates, and ensuring food security (Phillips et al., 2004). The application of antibiotics in food animals,whether for therapeutic, prophylactic, or growth-promoting purposes,has been instrumental in increasing global meat, milk, egg, and fish production, thus addressing the nutritional needs of a growing population (Darwish et al., 2013; Chen et al., 2019). However, the indiscriminate and excessive use of antibiotics in animal agriculture has created unintended consequences, most notably the presence of antibiotic residues in foods of animal origin and the global acceleration of antimicrobial resistance (AMR) (Bacanli & Basaran, 2019; Shahid et al., 2021). Antibiotic residues in food is the trace amounts of active compounds or its metabolites that remain in edible animal tissues, milk, eggs, and aquaculture products causes significant concerns for both consumer safety and public health. Even when below the maximum residue limits (MRLs) established by regulatory authorities, prolonged exposure may lead to allergic reactions, disruption of the gut microbiome, reproductive and developmental toxicity, mutagenicity, nephropathy, and in certain cases carcinogenicity (Ngangom et al., 2019; WHO, 2020). Furthermore, residues contribute to the selection pressure that drives the emergence and dissemination of resistant bacterial strains in humans, animals, and the environment (FAO, 2019; O’Neill, 2016). The One Health framework highlights these interconnected pathways, recognizing food as a critical link between veterinary antibiotic use and human AMR burden (Robinson et al., 2016). Globally, approximately 73% of antibiotics are consumed in food animal production, with projections estimating a further 11–67% increase by 2030,largely by intensification of livestock and aquaculture in developing economies (Van Boeckel et al., 2015; Tiseo et al., 2020). While industrialized nations have implemented bans on antibiotic growth promoters and introduced robust surveillance programs, most low- and-middle-income countries still facing challenges (Antibiotics, 2021; WHO, 2019). Consequently, foods containing antibiotic residues frequently enter local and international markets, undermining food safety standards and exacerbating AMR risks (Darwish et al., 2013; Antibiotics, 2021). Antimicrobial resistance (AMR) has emerged as a major global public health crisis, directly causing an estimated 1.27 million deaths and contributing to nearly 5 million deaths worldwide in 2019. Recognizing its severity, the World Health Organization (WHO) has identified AMR as one of the top ten threats to global health(Murray et al., 2022). By 2050, global economic losses linked to AMR are projected to reach USD 100–210 trillion, with Asia bearing a disproportionate mortality burden (O’Neill, 2016). Countries like India, with large livestock populations, rapidly growing aquaculture industries, and high human antibiotic consumption, represent critical hotspots where food, health, and environmental risks intersect (Chauhan et al., 2018; ICMR, 2020). Given these alarming trends, understanding the occurrence, detection, and consequences of antibiotic residues in foods of animal origin is paramount. This chapter explores the co-relationship between origin of antibiotic residues and AMR, regulatory measures aimed to safeguard food safety and strategies for prevention and control, framed within the One Health approach.

1.    ———— is a synthetic antimicrobial drug against M. tuberculosis.
    A.    Penicillin    B.    Tetracycline
    C.    Erythromycin    D.    Isoniazid
Ans.    D.
2.    Which of the antibiotic is not used as a food preservative ?
    A.    Pimaricin    B.    Nisin
    C.    Tylosin    D.    b-lactam antibiotic
Ans.    D.
3.    Which of the following bacteria is susceptible to thiophen-2-carboxylic acid hydrazide?
    A.    M. bovis    B.    M. tuberculosis
    C.    M. chelonei    D.    M. fortuitum
Ans.    A.

Introduction 
Antimicrobial resistance (AMR) is one of the most significant global health challenges of the 21st century. Defined as the ability of microorganisms— including bacteria, viruses, fungi, and parasites—to resist the effects of antimicrobial drugs, AMR undermines the progress of modern medicine. Antimicrobials—including antibiotics, antivirals, antifungals, and antiparasitics—are the cornerstone of modern medicine. Their discovery transformed the treatment of infectious diseases and enabled surgical advances, chemotherapy, and organ transplantation. However, Alexander Fleming, the discoverer of penicillin, warned that misuse could render these drugs ineffective. Antibiotics, since their discovery in the 20th century, revolutionized healthcare, saving millions of lives and enabling complex medical procedures. Yet, the misuse and overuse of antimicrobials in human health, veterinary practices, and agriculture have rapidly accelerated the emergence of resistant organisms. Today, this warning has materialized as a global crisis. Antimicrobial resistance (AMR) is now considered one of the top ten public health threats by the World Health Organization (WHO). It is not only a biomedical issue but also a socio-economic and developmental challenge. This chapter aims to provide an in-depth overview of AMR, focusing on definitions, history, mechanisms, causes, global status, impacts, and the urgency of mitigation efforts. It also explores national and global responses and future directions to curb the spread of AMR. AMR is estimated to cause 700,000 deaths annually worldwide, with projections of 10 million deaths annually by 2050 if urgent interventions are not implemented. In addition to health impacts, AMR poses grave threats to food security, sustainable agriculture, economic stability, and global development. This chapter provides a comprehensive overview of AMR, including definitions and conceptual frameworks, historical background, mechanisms of

Introduction
Antimicrobial resistance (AMR) is one of the top global public health threats. It is estimated that bacterial AMR was directly responsible for 1.27 million global deaths in 2019 and contributed to 4.95 million deaths (Murray et al., 2019).Despite its magnitude, public awareness of AMR remains low. Unlike tobacco smoke, antibiotics are potentially life-saving medications, but it is just as vital to understand the risks of overuse and their impact on future effectiveness in society as a whole (Langfordet al., 2019). Effective risk communication is essential to addressing AMR because it not only informs professional and community stakeholders but also nurturesbehaviour change across sectors. Within a One Health framework, risk communication connects human medicine, veterinary practice, agriculture, and environmental management, ensuring coordinated responses to reduce misuse and overuse of antimicrobials. This article explores the role of risk communication in combating AMR, highlighting global lessons, challenges, and strategies to strengthen communication systems for sustainable containment.
The Growing Threat of AMR
AMR occurs when microorganisms such as bacteria, viruses, fungi, and parasites evolve mechanisms to resist drugs that once effectively treated them. Misuse and overuse of antimicrobials in human health, livestock, aquaculture, and crop production accelerate resistance. According to WHO, AMR is projected to cause 10 million deaths annually by 2050 if left unaddressed (O’Neill, 2016). In countries like India, with high infectious disease burdens and large-scale antimicrobial consumption, the risks are particularly acute. Communicating these risks clearly and effectively is critical for influencing prescribing practices, agricultural use, and community awareness.Persistent misconceptions include beliefs that antibiotics are effective against viral illnesses and the mistaken notion that it is the human body, rather than bacteria, that develops resistance (Muflihet al., 2021). These knowledge gaps are often linked to insufficient or ineffective health communication (Krockowet al., 2023), sparse media coverage compared to threats like sepsis (Fitzpatrick et al., 2019).
Challenges in AMR Risk Communication
1. Low Public Awareness: Despite the global severity ofAMR, public awareness remains critically low. Surveys have found that a mere fraction of the population grasp its implications, with some reporting only ~8–11% demonstrating adequate understanding and many feeling powerless to contribute (Goshaet al., 2024).
2. Ineffective understanding of Terminology: It is recognized that AMR has a ‘language problem’ and the way in which healthcare professionals communicate about AMR may not always resonate with patients (Langford et al., 2019). Technical labels like “AMR” or “antimicrobial resistance” fall short in public communication. Experiments reveal that phrases such as “Antibiotic Resistance” or “Antibiotic Crisis” are more memorable and better at promoting critical behaviors—like completing prescribed antibiotic courses—than more formal terminology.
3. Misconceptions about Antibiotics: Widespread misunderstandings persist regarding antibiotics’ purpose and effect. Many incorrectly believe antibiotics treat viruses—common colds, flu, or even are effective painkillers (Gunasekara et al., 2022).Some think AMR occurs because the body becomes resistant, rather than bacteria evolving resistance(Muflihet al., 2021).
4. Complexity of the Issue: AMR is inherently multifaceted—spanning interactions among humans, animals, and the environment—which makes it challenging to distill into clear, actionable messages. Understanding Risk