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Site of incision: Detach the limb from the trunk by cutting various muscles viz; pectoral muscles, serratus ventralis, latissimus dorsi, brachiocephalicus, omotransversarius, trapezius, rhomboideus and long head of triceps.

Site of incision

Make a mid-lateral and mid-medial longitudinal incision on forelimb from elbow joint to 3rd digit. Joins the above incisions transversally on cranial aspect of elbow joint and 3rd phalanx, respectively. Remove the skin on dorsolateral aspect of limb

Site of incision-Make a rostro-caudal incision along the ventral border of horizontal ramus of mandible then extend cranio-dorsally along caudal margin of nostrils up to middorsal line of face. From the caudal end of 1st incision make another incision dorsally up to base of the ear then passes cranially along the ventral aspect of eye up to mid dorsal line of face. Reflect the skin carefully from ventral to dorsal, carefully remove the muscle zygomaticus, malaris and nasolabialis as these are firmly attached to skin.

Site of incision: Make a midventral incision on the sternum from cranial to caudal than from both ends of this incision make cranial incisions along the caudal border of shoulder and arm region to mid dorsal aspect of back and along last rib to mid dorsal aspect of back, respectively. Reflect the skin flap from ventral aspect to mid dorsal region of back.

Site of incision: Cut the skin over the back and loin region by making cranio caudal straight incision along the proximal part of 1st rib and the lateral margin of transverse process of lumbar vertebrae up to wing of sacrum and reflect the skin to mid dorsal aspect of back and loin.

Site of incision: Make a transverse incision below the anus between lateral margins of tuber ischia. Then from each end of this incision make an oblique incision caudo-cranially so that these join midventrally each other between the thighs. Remove the triangular flap of skin to expose the region.

Site of incision: Make a mid-lateral and mid-medial longitudinal incision on hind-limb from stifle joint to 3rd digit. Joins, the above incisions transversally on cranial aspect at stifle joint and 3rd phalanx, respectively. Remove the skin on dorsolateral aspect of limb.

Site of incision: Make a mid-lateral and mid-medial longitudinal incision on hindlimb from stiffle joint to 3rd digit. Joins the above incisions transversally on caudal aspect at stiffle joint above and 3rd phalanx below, respectively. Remove the skin on plantero-medial aspect of limb.

In order to evaluate the correct status of the organs their correct disposition within the body becomes very essential. Although one cannot see the organs beyond the skin, but appropriate knowledge of the topography of the organs can act as a glass covered watch wherein one can see from outside the arms and the dial of the watch.

Horse

Heart: The heart is placed in the middle mediastinum of the thoracic cavity with its long axis directed caudo-ventrally. Three fifth of the heart lies on the left of median plane while two fifth is on the right of the median plane. The base is placed dorsally, with its highest point at the junction of the dorsal and middle thirds of dorso-ventral diameter of the thorax. As a whole it lies opposite to second intercostal space/third rib to sixth rib/sixth intercostal space. The apex of the heart lies in the center of the last segment of the sternum about half inch from the sternal part of the diaphragm. Heart is directly related to the lateral thoracic wall at the cardiac notches formed in the lungs. The left surface of the heart extends from third to sixth rib in the lower third of chest while the right surface extends from third to fourth intercostal space.

Ultrasound is mainly used for reproductive purposes in bovine, now days it is also used for other applications. Ultrasound is an excellent tool for diagnostic imaging in large animals because it is portable, easy to use in the field, produces results at the time of the examination. It is totally non-invasive. Palpation of a gland can only detect certain levels of abnormality. Ultrasound can give an actual perception of mammary health, a picture of the inside anatomy and pathology. Tumors, scarring, stones, trauma, hematoma formation and level of damage due to mastitis can all be evaluated by this technique. Other diagnostic tools are still primary diagnostic tools, ultrasonogram gives yet another dimension to the hard to diagnose case. It will also allow a better estimate of treatment prognosis to aid in deciding whether to cull or treat a dairy animal.

Congenital anomalies at initial stages of the mammary gland development during gestation may result into presence of supernumerary teats or less number of external teats, in place of usual presence of four teats in buffaloes. Hence, cases with two / three / five or six teats are often encountered in the f ield.

Congenital anomaly of newborn buffalo calves is quite common in new born calves. Most common encountered are atresia ani, rectovaginal fistula etc. Sometimes, developmental defects interfere with physiological processes viz. in urination and defecation. Therefore, such cases, if not corrected well in time can cause death of the patient. In the present report, few newborn calves were brought to the veterinary clinic with the following abnormalities:

Agenesis of Urogenital System Without Anus

Five newborn buffalo calves were brought to the veterinary clinic with the complain of not passing urine and feces. There was no visible external genitalia and anal opening. At the site of vulva, there was only an impression of vulvar lips and below it, a cord like structure was hanging.

There was an impression of round structure below the skin; in some cases it was outside giving appearance of scrotum which was located in between the two thighs. On ventral side of abdomen, preputial sheath was not present, therefore, it was very difficult to decide about the sex of the calves and site of external preputial-orifice. There was an indication of both sexes, but organs were not developed, therefore it was difficult to decide about the sex of the calves. In some cases, impression of the anal sphincter was visible but there was no visible external anal opening. There was slight deviation of the tail from its base also. Intake of milk was normal. Abdomen was distended bilaterally. Respiratory rate was higher than the normal with slight discomfort .Heart rate was elevated. Blood picture revealed increased total blood cell count with neutrophilia. Radiography of the abdomen showed distended urinary bladder at the floor of pelvis indicating formation of the urogenital system without development of the external opening. Barium meal study revealed marking of gastrointestinal tract and stagnation of the barium up to the rectum.

Ultrasonography in sheep is becoming popular in the field of reproduction and replacing the other old-age techniques, viz. abdominal ballotment, recto- abdominal probes, X-rays and radioimmuno-assays. The reasons are numerous such as unsatisfactory results, hazardous effects to animal, operator or environment, high cost, long delay in diagnostic results and requirement of sophisticated labs. The ultrasound system has low operating cost and does not need special labs as it can be done at farm itself. Ultrasonography is considered safe to sheep and the operator and provides quick and reliable results. The popular opinion about ultrasonography is that it is noninvasive imaging system and is rapid, safe and practical means of diagnosis of pregnancy in sheep. The B-mode real time ultrasonographic system particularly portable system is popular among sheep breeders. In sheep, B-mode real time ultrasonography was used in 1971, using 5 MHz frequency transducer. A transducer of switchable frequency between 7.5 to 5.0 is more popular in sheep practice.

Transrectal Procedure in Sheep

The procedure is similar to goat, but sheep remain quite during transrectal ultrasonography than goat. Following are some of the steps to obtain good quality image:

Corpus luteum, one of the biological clock of the estrous cycle and pregnancy, is known foremost for its production of progesterone that blocks the pituitary release of gonadotrophins and prepare the uterus for pregnancy. The ovarian follicle wall consists of granulosa cell and theca cells vascularised and luteinize after ovulation to from a corpus luteum. Corpus luteum is temporary steroid- producing gland undergoes marked structural and functional changes far a short time span during its development functional life and regression. During estrous cycle, the corpus luteum undergoes an initial period of growth (metestrus), followed by a period of maximal function (diestus) and finally by a period of regression (proestrus) preceding the next ovulation (Robert, 1986). The most prominent cell in the CL is the large luteal steroidogenic cell.

Ultrasound imaging has been used to detect and measure the corpus luteum throughout the estrous cycle and corpus luteum has been characterized during pregnancy and non pregnancy. Changes in corpus luteum diameter as detected by ultrasonography are highly correlated with progesterone production. A high correlation has been found in the ultrasonographic detection of bovine corpora lutea (Lean et al., 1992) and between luteal tissue area measured ultrasonographically and plasma progesterone concentration (Bergfelt et al., 1989).

Key Points

• Sudden severe bleeding or hemorrhage can cause hemorrhagic shock and death.

• Chronic and or occult bleeding or hemorrhage can cause anemia and even death.

• Common causes of bleeding include trauma, surgical incisions, extreme temperatures, vitamin K deficiency, medicines, and toxins.

Introduction

Bleeding is the extravasation (arterial, venous, or capillary) of blood caused by trauma or a surgical incision.

Key Points

• Frostbite occurs when exposed to freezing temperatures. Animals that are weak and malnourished are more likely to be injured.

• It is a prevalent condition throughout the winter, particularly in animals who do not have shelter or have inadequate protection to protect them from the cold.

• Excessive cold can harm skin and subcutaneous tissue. In severe circumstances, injuries may be irreversible.

Introduction

Frostbite, also known as freezing cold damage, is caused by prolonged exposure to temperatures below 0º C. Exposure to freezing temperatures causes excessive heat loss, disrupting the body's heat balance and resulting in systemic or local acute freezing injury. Frost bites cause superficial tissue injury, but in extreme cases, gangrene develops in the joint or synovial sheath, and the animal dies from toxemia. However, all animal species are susceptible. It occurs rarely in healthy and well-nourished animals. Animals that have lost their coats or are malnourished are particularly vulnerable to harm. Prolonged exposure to colder temperatures increases the risk of frostbite and the severity of the injury. In the majority of cases, frostbite occurs in animals that are left in winter.

Key Points

• Animals can experience varying levels of electric shock. Lightning typically kills animals instantaneously and causes them to fall to the ground without struggle.Most electric shock deaths are caused by cardiac or respiratory arrest.

• Animals often recover from minutes to several hours, rarely going unconscious. Residual nervous system effects, including sadness, paraplegia, and increased cutaneous sensitivity to painless stimuli, can last for days, weeks, or even months.

Introduction

Lightning strikes, broken transmission lines, malfunctioning electrical circuits, or an animal chewing on an electrical cable can all cause an animal to be injured or killed by high-voltage electrical currents. Lightning strikes are seasonal and occur in thunderstorm-prone regions. Caution should always be maintained when investigating probable electrocution situations because the source of the electrification, such as broken transmission wires, may still be present.

Key Points

• Physical cooling is more effective in managing hyperthermia.

• Heat stroke occurs when the cause is solely physical.

Introduction

Hyperthermia is an unusually high body temperature (also known as overheating) caused by the body absorbing or producing more heat than it can discharge. Simply said, it is the increase in temperature above the critical point. Heat stroke is most common in animals when they lose control of their body temperature.

Key Points

• Hypothermia occurs when an individual's body temperature drops below its critical level.

• Excessive heat loss or inadequate thermoregulation can lead to this condition.

• It can be minor or severe. Mild hypothermia is harmless, while moderate to severe hypothermia is an emergency.

Key Points

• Constipation can be caused by a variety of clinical disorders or as a subsequent consequence.

• Rehydration and gentle lubrication of the GIT tract with oral paraffin or linseed oil will alleviate constipation caused by feed and water.

• Small animals, especially cats, are more prone to constipation.

• Common treatments for constipation include dietary changes, enemas, and mechanical removal under anesthesia.

Introduction

Constipation occurs when the ingesta moves slowly or not at all. It is a sign of several intestinal disorders. Although not all extra-gastrointestinal illnesses cause constipation, some do. A systemic illness is characterized by parasympathetic nervous system depression and constipation. Many diseases can lead to constipation as a complication. Chronic constipation in dogs leads to obstipation, which is characterized by drier, firmer, and more compact stools. Dogs cannot defecate and hence require medical intervention, such as surgery or manual feces evacuation. When the colon becomes irreversibly swollen and hypomotile (megacolon), surgery may be necessary. As a result, corrective actions for constipation should be implemented as soon as they are discovered. This will aid in a quick recovery from disease. The current chapter provides an overview of constipation in general and its management.

Deep freezing or cryopreservation of semen refers to freezing and storage of semen at –79ºC using dry ice and alcohol or at -196ºC using LN2 as refrigerant. Cryopreservation of semen was first attempted by Parkes in 1945. But cryopreservation of semen received a boost by discovery of glycerol a cryoprotectant by Polge in 1949.  Bull semen was first successfully frozen by Stewart in 1951. Cooling rate is a vital factor determining the post thaw survival of the sperm cells. Cell death occurs in rapid freezing due to formation of intracellular and possibly extracellular ice. In contrast slow cooling rates cause cell death due to diffusion of intracellular fluid into the extender, which then become increasingly hypertonic as the extender’s water freezes causing intracellular osmotic damage as the cell dehydrates. Hence, the cooling rate needs to be balanced in a way that it is neither too fast to cause cell death due to cold shock nor too slow to result the same due to osmotic shock. Ultra rapid freezing gives excellent morphological preservation but with poor retention of functions on thawing due to large number of minute crystal formation.