eChapters

The action of the sex hormone is not confined to the growth and development of the sex organ alone, but the normal body growth as well as the growth of the skeleton are to a great extent influenced by these hormones. It is well documented that following oopherectomy, female experience a “rapid phase” of bone loss for the next few years. Of the female gonadial hormones, the action of estrogen on skeletal growth has been studies by various workers and shows that estrogen replacement therapy can help to prevent the postmenopausal stage of accelerated rate of bone loss. The effects of progesterone on bone healing or bone growth has not yet been studies in detail. The objective in this chapter is thus undertaken to investigate the effects of progesterone supplement on cortical bone healing in spayed rabbits.

The study is conducted on ten clinically healthy female adult New Zealand White rabbits divided into two equal groups (A and B) consisting of five animals each. All animals are spayed and kept in standard diet and management throughout the experiment. In all animals, midshaft simple transverse fractures are created in both the ulnae under thiopental (2.5%) anaesthesia. In test group (gr A), 1mg (=1ml) of progesterone is injected subcutaneously in alternate days for upto 35 post fracture days. Lateral and anterio-posterior radiographs are taken on days 0, 7, 14, 21, 28 and 35 post-fracture. These radiographs are observed for progress of fracture healing and complications, if any, at different intervals.

Standard postoperative management of animals with fractures includes the use of nonsteriodal anti-inflammatory drug (NSAID). Nonsteriodal anti-inflammatory drug inhibits inflammatory responses by blocking prostaglandin (PG) synthesis via the Cyclooxygenase pathway of arachidonic acid metabolism. However, PG is important factor in early phase of bone healing. A local increase of PG concentration is a response of bone to trauma and PG (particularly PG₂) may stimulate differentiation and proliferation of osteoprogenitor cells during early bone healing. It has been reported that NSAID, such as Diclofenac acid, ibuprofen, indomethacin, aspirin, salicylic acid decreased ingrowths of bone into porous coated implants and decreased mineral content in rabbits. In view of the above fact, this chapter is undertaken to investigate the effects of one potent NSAID (Flunixin Meglumine) on fracture healing.

The study is conducted in ten clinically healthy adult New Zealand White rabbits of either sex divided into two groups (A and B) consisting of five animals each. In all animals, mid-shaft simple transverse fracture is created in both the ulnae under thiopental (2.5%) anaesthesia. In animals of group A, 0.25ml (12.5 mg) Flunixin Meglumine (Ilium Flunixil) is injected intramuscularly on alternate days from day 5-20 postoperatively whereas, in group B no drug is given. Lateral and anterio-posterior radiographs are taken on day 0, 10, 20, 30 and 40 post-fracture. The radiographs are observed for progress of fracture healing and complication, if any, at different interval. The specimen for histopathological study is collected from the healed site of test bones immediately after euthanizing the animals on day 40. Every specimen is consisted of both healing and adjacent host bone. The bone sections are preserved in 10% formalin for 9-10 days and then are decalcified using Goodling and Stewart’s solution (15% formic acid, 5% formalin and 80% distilled water). Completely decalcified sections are then processed routinely for H&E staining. These sections are observed for the status of healing at the fracture site, at cellular level.

Uterine torsion in cow is actually the rotation of the pregnant uterus on its longitudinal axis and usually occurs before onset or during late first stage of parturition but rarely encountered during early second stage of parturition. In cattle, uterine torsion involves both gravid and non gravid horns because of strong intercornual ligament and distension of uterine horn and body with placenta and fluid. The remarkable feature of uterine torsion is its association with advanced pregnancy and process of parturition in cow.

Estrus is referred to as the period of estrous cycle during which the female animal allows the male for mating. It is the period of estrous cycle during which insemination is done to female animal so that the animal will become pregnant. During estrus, cow usually seeks out the male and ’stands’ for him to mate her. In all domestic species, Ovulation occurs during this phase of the cycle but in case of cow, ovulation occurs about 12 hours after the end of estrus period.

Vaccination of cattle and prevention of diseases causing huge economic losses is very much essential for economic rearing of livestock as prophylactic and strategic vaccination helps in development of immunity against the vaccinated diseases thereby prevents the animals from developing such infections and consequent diseases. Animals should be vaccinated at specific age and at definite time intervals for optimal protection against the diseases.

A. Triglycerides Clinical significance To form lipoproteins, triglycerides combine with cholesterol, phospholipids, and plasma proteins. To determine whether hypertriglyceridemia may be present, the serum triglyceride concentration is measured (increased blood and serum levels of triglycerides). Chylomicrons and very low density lipoprotein (VLDLs), which cause the extensive lipaemia frequently observed in serum or plasma samples, are both mostly composed of glycerides.

Calcium Clinical Significance Calcium is an essential mineral which is involved in many body systems. These include the skeleton, enzyme activation, muscle metabolism, blood coagulation and osmoregulation. In the blood calcium exists as 50% ionized, 40% protein bound and 10% complexes with anions such as citrate and phosphate. Only ionized calcium is biologically active in bone formation, neuromuscular activity, cellular biochemical processes and blood coagulation. Factors governing the total plasma concentration are complex and include interaction with other chemical moieties, proteins and hormones. Calcium, phosphorus and albumin metabolism are interdependent.

Creatinine Clinical significance Creatinine is produced at a steady rate due to muscle catabolism and is not reabsorbed by the kidney tubules after filtration. Its measurement provides an indirect assessment of the glomerular filtration rate (GFR). Unlike urea it is not markedly affected by diet or any aspect of liver function and therefore the test is more specific for renal dysfunction

Purpose of study An essential component of the diagnostic process in the evaluation of the disease process is the laboratory diagnosis of any parasitic infection. Sometimes, this confirms the presumptive clinical diagnosis, and other times, it provides evidence that the clinician may have ignored. A sufficient specimen must be used for the examination to achieve a satisfactory diagnosis. Internal parasite testing needs to be done often. Regular inspection typically enables early parasitism identification, preventing gross infection. The majority of the parasites can be accurately identified by doing a detailed examination of the faeces. Faecal sample collection and delivery for parasite identificatio

A. Intestinal protozoa Fresh faeces must be used as a sample. The sample must be examined immediately and without refrigeration. Methods of examination 1. Direct smear examination A thin smear of faeces mixed with either water or normal saline (recommended for Trophozoites since they stay intact and motile longer). Select blood-tinged mucus or tiny tissue bits if the faeces are dysenteric. One to two percent of an aqueous eosin solution can be used in place of saline or water, but the oocyst still resist the stain. The viability is examined using the eosin solution. It is possible to employ an iodine solution (which kills protozoa), which allows for the identification and differentiation of the protozoa. It is possible

Puberty • It is the age at which sexual organs are functionally developed and gonads start responding to GnRH to start steroidogenesis and spermatogenesis. Animal at puberty develops secondary sexual characters with proper libido. • At puberty, the bull is able to produce 50 million spermatozoa with 10% progressive motility in 1st seminal ejaculates. It should be 100 million spermatozoa with 10% progressive motility in 1st seminal ejaculate in case of stallion. • At puberty, gonadotrophic cells are functionally developed in the mature pituitary tends to gonadal maturation. The steroidogenesis starts properly which leads to the development of genital organs and secondary sexual characters.

The essential database for all skin diseases consists skin scrapings. Skin scrapings fall into two main categories: superficial and deep. Superficial scrapings give information from the epidermis’s surface without causing capillary bleeding. Capillary haemorrhage is a sign that the sampling was deep enough to gather material from the hair follicle. Deep skin scrapings are used for this purpose. The main purpose of skin scrapings is to detect the presence or absence of mites. A skin-scraping spatula, which is a thin metal weighing spatula, is recommended for use while scraping the skin. These spatulas are safe and can be used again. Scrapings from active lesions at several places are to be submitted. Scraping from active lesions at several places is to be submitted. Generally, the periphery of the lesion contains more parasites than the centre. Skin scrapings are used to identify ectoparasites and fungal diseases.

Libido • It is the willingness & eagerness of male animal to mount and perform complete service. It is sexual desire or drive which is an innate character of male animals. • Libido and mating ability have no direct relation with fertility. Libido is not related directly to sperm production and semen quality. • The libido of an animal depends on androgenic steroid hormone. Vasectomy or cryptorchidism does not affect libido in male animals. • Animals have no or reduced libido that are castrated before puberty. If the animals, that know the copulation, are castrated after puberty, they display the copulation behaviour for longer period. • Reaction time – It is the time between the contact of a male animal with female animal to 1st false mount with erected penis. Reaction time is the good predictor of libido.

Definition

Humerus is long selender and has slight spiral twist.

Oblique type of fracture is most common in diaphyseal region.

It come under higher level of fracture.(Fig. No-1)

Appropriate method of fixation is by intramedullary pinning and modified thomas splint.

Definition

Fracture of femur comes under higher level fracture (Fig. 1) where the appropriate treatment is intramedullary pinning and modified Thomas Splint.

Requirement

Parentral sedative

Local anaesthesia

6mm 1fit long intramedullary pin (Fig.No-3)

Electric bone drill

Bone hammer

Pin cutter

• These are the injuries occurred to male animal at the time of natural mating. These injuries do not occur only to genital organs but also to other organs or muscles. • Most coital injuries occur to male animal with good libido (high testosterone level) but having aggressiveness and poor experience of mating. Such animals tend to quick response in the presence of estrous female. Some of the coital injuries are: 1. Balanitis and Posthitis • Trauma to the penis and prepuce which later on leads to infection and inflammation. • This occurs especially in young inexperienced bulls with great sexual desire • Adhesion may lead to phimosis or paraphimosis condition • IBR-IPV infection from cow genitalia after mating results in pus formation and necrosis of the penis and prepuce. • Prepucial eversion leads to laceration and bleeding.

• These are the bad habits of the male animal due to which ultimately their maximum reproductive potential cannot be utilized. • There are so many vices seen in male animals like stall walking, weaving, cribbing, wind sucking, crib biting, viciousness etc. These behavioural disorders are mostly due to improper managemental practices like abusive handling, poor exercise, irregular semen collection and poor exposure to female etc. These may be also due to hormonal imbalance or nervous disorders. - Some important vices which are more related to reproductive behaviour are: 1. Masturbation (Onanism) • Bulls masturbate by rubbing their prepuce and penis against any object. • Boar masturbate and ejaculate by inserting the penis into the preputial diverticulum called Balling up • Boar may show rectal copulation and leads to ejaculate : Pederasty

There are some methods to control male reproductive behaviour for better breeding and managemental practices i.e. Vasectomy and Caudal epididymectomy, Penile surgeries, and castration etc. • Surgical and non surgical interventions in the male reproductive system are carried out for: • To make teaser bull • To make bull sterile • To prevent the transmission of venereal diseases • Population control or Breeding management • To rectify any ailment or deformity Teaser Bull • A male animal whose capability of spermatogenesis as well as libido is maintained but it is not able to impregnate a female. • For the preparation of teaser bull or Gomer bull, Male animal should be selected well before the start of breeding season. Bull should be young vigorous and fertile. It should be of moderate adult size with mild temperament. Heavy and large sized bull is tough to handle, needs more time for surgery, has chances of more hemorrhage, and may have more post surgical complications.

Etiology

 Acral lick dermatitis is usually observed on extremities of dogs and the probable etiology is persistent licking. It is also caused due to self-trauma or mutilation.

Etiology

The condition is usually caused by implantation of low virulent saprophytic bacteria due to traumatic injury. These organisms stimulate cell mediated immune response by persistence of antigen in the tissue.