eChapters

Key Points

• It might be necessary to administer oxygen or perform artificial respiration. Giving tranquilizers is not a good idea.

• Precaution: It's crucial to always follow the label's instructions on how to use pesticides since they provide information on how to do so. Warnings against using on unapproved species or in testing conditions must also be included on labels. A maximum of care should be made to prevent drift or drainage to neighboring fields, pastures, ponds, streams, or other properties outside the treatment area. In no case should amounts larger than those specifically recommended be utilized.

Introduction

It has been determined that a number of plant species are poisonous to animals. These plants not only posed a health risk to humans and animals, but they also significantly reduced cattle productivity. Many plants contain chemicals that can be dangerous or even lethal to animals. The degree of toxicity varies based on the animal species, the quantity of plant material ingested, and the particular poisonous compounds in question. In general, there are two categories of dangerous plants: those that are known to be toxic and contain toxic compounds, and those that are normally non-toxic but can turn hazardous in certain circumstances. Here are some typical plant toxicities, along with information on managing toxicity and clinical signs.

Indication -To know the method of POP bandaging technique.

Method of preparation of POP bandage rolls-

A gauze bandage of 6" or 4" width is spread on a clean floor. Plaster of paris powder is spread uniformly over the entire length of bandage & is folded into roll bandage. In this way 4-6 bandage rolls are prepared.fig-1(a)&(b)

Method of preparation of pop bandage splints

The length of the prepared pop splint is determined by measuring the entire length of fractured bone along with the joint below and above the fractured site) are measured. This much of length of gauze is spread for splint preparation.

POP powder is impregnated upon gauze. The gauze is again spread over this much of length in this manner sprinkling of POP powder & overlapping gauze bandage upto 4-6 layer are continued [fig-1 (b)]

Definition

Compound fracture is a type of fracture in which a broken piece of bone protrudes to outside rupturing the skin with a risk of further infection.

Procedure of management

Clean and flush thoroughly the exposed bone using sterile NS followed by sterile medicated NS (NS with antibiotic or antiseptic). Figure (1)

Mop dry the wound thoroughly.

Apply topical antiseptic or antibiotic.

Put the bone inside the part.

With a sterile artery forcep fix a flushing catheter within the wound at its higher level. Figure (2)

Cover the wound over fracture site with sterile soft padded absorbent cotton padding. Figure (2)

The joint below and above of the fracture site covered with thick layered padded cloth before application of POP cast. Figure (3)

POP cast is wrapped as per the conventional procedure over the fracture site covering the joint below and above. Figure (4)

Large animal orthopedic patients are often managed with modified Thomas splints and to achieve an acceptable outcome with this device the following points should be kept in mind.

Animals initially find discomfort with the splint and try to reject it by kicking and often show violent movements. Hence initial acclimatization with the device by close observation and supervision of the patient is essential.

Recumbent animals should be supported in standing position by a sling and they should be managed with the principle “12 hrs on sling and 12 hours on floor”.

Movement of the animal should be restricted.

Surgical Plan
i. Pre-operative Course of Action 
    a.    Client communication.
    b.    Clinical laboratory evaluation, radiograph, treatment for fluid or blood volume deficits. 

ii. Intra-operative Plan of Action 
    a.    Surgical approach 
    b.    Material and equipment needed 
    c.    Technical and support personnel

Preanesthetic agents are so named because they are usually given to prepare the patient for administration of an anesthetic agent for one or more of the following reasons:

    1.    To reduce the amount of general anesthetic needed and increase the margin of safety.
    2.    To calm the patient so that anesthesia can be administrated without fright or struggling. 
    3.    To reduce secretions of salivary glands (antisialogogue) and mucous glands of the respiratory tract, thus maintaining a free airway. 
    4.    To reduce gastric and intestinal motility and prevent vomiting while the patient is under anesthesia. 
    5.    To block the vagal reflex, thus prevent cardiac slowing or arrest (bradycardia).

Liquid nitrogen and cryocan

Several cryogenic agents like solid carbon-di-oxide (-79^ºC), liquid air (-183^ºC), liquid helium  (-263^ºC) and liquid nitrogen (-196^ºC) have been used in many countries for freezing and preservation of bovine semen. Liquid nitrogen is fourth coldest substance known and its small amount is converted into a very large amount (near about 700 times) of its volume when exposed to atmosphere. It is a colourless, odorless, non-irritant, non-toxic, non-caustic, non-corrosive, non-inflammatory and inert gas having boiling point of -196^ºC. Being a liquid it comes into good contact with the surface of the semen straws, constant storage temperature is maintained throughout the straw, and due to these properties LN2 is most widely used refrigerant for semen cryopreservation in the world. 

Precision feeding aims to supply animals with appropriate nutrients according to their changing physiological state, growth, production and environment. Conventional feeding plans are often static and may lead to waste, increased costs and environmental losses. This chapter introduces an AI-enabled framework integrating animal data, feed composition, growth characteristics and external factors. Regression, ensemble learning and other machine-learning models predict feed intake and weight gain, while linear programming and metaheuristic optimization generate efficient allocation plans. A decision-support system provides recommendations in real time. The approach can improve feed conversion, nutrient utilization, profitability and sustainability while reducing overfeeding, resource wastage and the environmental impact of livestock production.

6.1 Age

Age is one of the most important risk factors for developing mammary tumors in dogs. A comprehensive study carried on dogs has found that the risk of mammary tumors become significant by 8 years of age and increases linearly thereafter. The age of onset of tumors, however, may vary depending on the life span of the dog breed. An average age of CMT onset is 8-11 years, indicating as a condition of the middle-aged or older dogs. Mammary tumors are generally rare in dogs under 3 years of age, unless the dogs have been given exogenous hormones such as progestins

6.2 Breed

Most consistently listed breeds in which mammary tumor occur include, English springer spaniel, Brittany, Cocker spaniel, English setter, Pointer, Afghan hound and German shepherd, in addition to the smaller breeds such as Poodle, Maltese, Chihuahua, Beagle, Dachshund and Terriers. These studies clearly show that the risk for mammary tumors has some breed association and, therefore, having a genetic component. Pure breeds of dogs are generally at a greater risk i.e., known to account for 80% of the submitted cases. Some studies have further shown that certain lines within specific breeds are at more risk than others.

Accurate early diagnosis of pregnancy is essential for successful breeding programme.

Ability is required for successful practice.

Skill is to be developed to indicate the duration within weeks in early and months in late pregnancy.

The diagnosis of pregnancy (Cyesiognosis) was desired earlier by farmers for curiosity, but currently early pregnancy diagnosis is considered essential for economically viable and profitable dairy farming. Clinical methods are commonly used for pregnancy diagnosis in cattle with recto genital palpation and trans-rectal ultrasonography as the methods of choice for an accurate and early pregnancy diagnosis.

Introduction

For agricultural applications as well as for research work, often we need to prepare solutions of different agrochemicals of varying strength, as per the requirements. Agrochemicals are used to help crop plants grow, produce, and protect them from biotic/abiotic stresses. Many of the agrochemicals (insecticides, herbicides, fungicides, rodenticides, molluscicides, nematicides, etc.) are used to protect the crop from insect pests and diseases. However, some of them (fertilizers and soil conditioners) are used to augment crop yield. While in the previous chapter (Chapter 8) we discussed about preparation of laboratory solutions, in this chapter we will briefly discuss about the preparation of different agrochemicals.

Agrochemical/agrichemical is a generalized term used for the chemicals applied in agriculture. In general, it also includes the feed-supplements used in f isheries and animal husbandry.

Agrochemicals

Fertilizers

Fertilizers are used to augment growth of crop plants and to mitigate nutrient deficiency in the soil. Fertilisers can be organic (containing natural substances, prepared through natural processes) and inorganic fertilisers (synthetic fertilizers, manufactured artificially using chemical processes using natural deposits, by chemical alterations).

Soil Conditioners

Soil conditioners include manure, livestock and leaf compost, peat that help making the soil in good condition. They are laid on the top soil and then mixed properly. They not only increase nutrient contents in the soil but also improve aeration and water holding capacity.

Introduction

Different types of culture media are used for culturing microbes and plant tissues. Preparing as well as sterilizing them properly following appropriate method with safety for the desired purpose are important parts of research work. In this chapter, we will discuss briefly about different types of media, their composition, preparation and sterilization in laboratory for microbial or plant tissue culture.

Culture Medium

Ingredients mixed to prepare solid, liquid or semi-solid substance to support the growth of microorganism or plant tissue in vitro (outside the body) is called culture medium. Frequently, it becomes important to grow microorganism or plant/animal tissue in vitro for any or all of the following purposes:

• To study characteristic features or properties of microorganisms.

• To identify the causal organism(s) of infection in a clinical sample, so that appropriate remedy can be determined.

• To culture/multiply microorganism or plant/animal tissues in vitro for research or commercial purposes.

• To prepare/produce biological molecules, vaccines, antigens, proteins, metabolites, etc. in vitro for research or industrial purposes.

Aim

To know the methods of preparation of side bars for large animal Thomas splint fabrication

Procedure

Two strong dry matured bamboo splints are of more than actual length of Thomas splint are prepared, (figure 1)

Three nicks at a distance of 2 inch are grooved on the side of the side of the splint at one end of the splints for keeping the tying end of the ropes in position (figure -2)

The bamboo splints at the lateral ends are thinned to some extent. For knob kniiting of splint to the metal ring by making an arc (figures).

All the steps are depicted in figure 1 -4.

Before fixing the metal ring with the bamboo extention splint the thinned ends are emersed inside water for easy arching of the bamboo splint.

The Tris citric acid egg yolk glycerol extender is the most frequently used extender in the cryopreservation of cattle and buffalo semen. Preparation of the extender plays a very important role in determining the quality of the processed semen. Therefore, care must be taken to ensure that the extender is prepared aseptically and it contains all the ingredients in appropriate concentration. Extender should always be prepared under a laminar air flow. It is also important to check that the pH and osmolarity of the semen extender for a particular species corresponds to the prescribed limits for that particular species. The Tris citric acid egg yolk glycerol contains the following ingredients:

In Doppler imaging, the reflections are from circulatory vessels, while in 2D ultrasonic image the reflection is from a tissue(s) or organ(s) In 2D ultrasound strength of reflection in B-mode depends upon density of the organ.On other hand the reflection in Doppler imaging depends upon direction of the circulation. If circulaltion is coming towards transducer, the signal will be strong, while it looses its strength while flowing away from the transducer. As shown in the diagram, the position of transducer on curved surface decides the strength or amplitude of signal. If it is positioned on curve end from where circulation is coming, the signal will be of higher amplitude, but when position is changed to other end of curved surface, where circulation is going down the amplitude of signal will decrease accordingly and is shown in reverse direction.

Principle of Doppler

Movement of blood in vessels is detected, which is depicted by color in some ultrasound machines. The flow coming towards transducer is shown above baseline, while flow going down i.e. away from transducer is shown below baseline. If position of transducer is changed from where the arterial flow is going away from transducer then, the arterial flow will be shown below baseline and venous flow that is coming towards transducer will be shown above baseline. Some machine shows red and blue color of the flow. The red color shows flow coming towards and blue color shows flow going away from transcer. A beginner ultrasonographer may think that the red color is from artery and blue is from vein. This is not true, this shows direction of flow. Since ultrasonic image of flow in Doppler is formed from series of pulses returning from vessels, the magnitude of pulse changes with variation in flow. A stationary flow will not show any height of magnitude.

Radiation therapy is the delivery of ionizing radiation to a specific body areas with the goal of obtaining maximum lesion control with minimal complication. Radiation therapy is based on the fact that ionizing radiation is lethal to the cells, particularly rapidly dividing ones. Normal cells in the irradiated area may be as sensitive to ionizing radiation as tumor cells. Therefore, the normal tissue response limits the amount of ionizing radiation that can be tolerated by the patient. Effective use of radiation therapy requires an understanding of the physics of the radiation used and normal cellular and tumor biology as it relates to radiation therapy as well as an appreciation of the clinical application of veterinary radiation therapy.

Radiographic positioning refers to placement of the body in respect to linear alignment of X-ray tube and film. Correct positioning is a most important prerequisite to obtain a diagnostic radiograph. Misinterpretations can result from inaccurate positioning.  Label the film before exposure with anatomical lead markers/ X-rite tape. The anatomical markers should be placed in close proximity to the area of interest without overlying important structures. At least two exposures of the part at 900 to each other should be taken to get complete anatomical information of the part.