eChapters

General Instructions

The monitor of ultrasound machine should be placed at the eye-level. The daylight should not be too bright. The setting of the machine should be done to obtain best picture on the screen. There are various knobs on the machine to set contrast and brightness. There are three other knobs that are used to set near gain, far gain and overall gain. There is also a brightness bar (help-bar) on the screen with different brightness areas. These knobs should be turned clockwise or anti-clockwise, so that all the areas on the help bar (brightness bar) are visible.

Transrectal Procedure

In cattle, trans-rectal ultrasonography is done in a way, similar to rectal examination done for palpation of genital organs. Following steps are suggested for proper procedure

1. Properly restrain the animal.

2. Evacuate the feces from the rectum.

3. Select a transducer of proper frequency (the linear array transducer of 5.0 MHz frequency is useful for general purpose in bovines, however for detailed examination of early conceptus and small follicles, a transducer of 7.5 MHz can also be used. Some transducers have range of these frequencies in single-equipment. Such transducers of switch-able frequency are found more useful.

Vaccination of cattle and prevention of diseases causing huge economic losses is very much essential for economic rearing of livestock as prophylactic and strategic vaccination helps in development of immunity against the vaccinated diseases thereby prevents the animals from developing such infections and consequent diseases. Animals should be vaccinated at specific age and at definite time intervals for optimal protection against the diseases.

Definition

Wound with infection with 6 hours of occurrence can be brought to non infected stage by flushing.

Procedure

The infected wound at fracture site is cleaned / flushed thoroughly using sufficient quantity with antiseptic solution.

The site is applied with topical antiseptic / antibiotic and the size of the wound is reduced by placing interrupted stitches for skin closure.

For drainage of inflammatory fluid, the counter opening is made as the distal part of the swelling of the dependent part of flushing catheter is placed inside to carry out regular flushing of the wound without removing the immobilizer.

The fracture site is immobilized as per the conventional procedure using POP cast leaving two window over the opening of counter opening site.

Puberty • It is the age at which sexual organs are functionally developed and gonads start responding to GnRH to start steroidogenesis and spermatogenesis. Animal at puberty develops secondary sexual characters with proper libido. • At puberty, the bull is able to produce 50 million spermatozoa with 10% progressive motility in 1st seminal ejaculates. It should be 100 million spermatozoa with 10% progressive motility in 1st seminal ejaculate in case of stallion. • At puberty, gonadotrophic cells are functionally developed in the mature pituitary tends to gonadal maturation. The steroidogenesis starts properly which leads to the development of genital organs and secondary sexual characters.

Puberty and sexual maturity

Puberty is defined as the age at which reproductive function is initiated in animals. This corresponds to changes in hormone levels and the ability to function sexually in order to reproduce. The puberty is the period when the sexual organs are functionally developed, libido is prominent and the male is able to produce the spermatozoa and is able to copulate resulting in pregnancy. The onset of puberty is a gradual process and occurs after achieving optimum body weight, which is influenced by plane of nutrition, genetic differences, management practices, breed, diseases and individual differences.

Pyometra meaning “pus filled uterus” is a condition in cow which is characterized by progressive accumulation of pus in the uterus, presence of persisting CL, absence of estrus and regular estrus cycles and a closed cervix. Pyometra occurs following chronic endometritis in most cases as uterus fails to produce endogenous CL regressing agent i.e. PGF2a. So, the CL of diestrus persists resulting in failure of elimination of uterine infection as the animal remains under the continuous influence of progesterone. Progesterone production from the persistent CL causes closure of cervix, pus accumulation in the uterus and consequently leading to enlargement and distension of uterus.

1. Introduction:- Overview of Good Laboratory Practices (GLP)
1.1 Definition and Scope of GLP
GLP refers to a set of standards that govern the organizational processes, personnel responsibilities, and conditions under which laboratory studies are conducted. It aims to regulate research environments and ensure the generation of high-quality, traceable, and reproducible results. The scope of GLP encompasses a wide range of scientific fields including pharmaceuticals, agrochemicals, veterinary products, and environmental safety assessments. Good Laboratory Practices (GLP) regulations were developed by the Organization for Economic Co-operation and Development (OECD) and well adopted by regulatory agencies world-wide. The primary objective of GLP is to promote high standards of accuracy and integrity in non-clinical research to protect human health and the environment.
1.2 Principles of GLP
The fundamental principles of GLP include:
• Study Planning and Protocol Development: Every study must have a clear plan and defined objectives, which should be outlined in a comprehensive protocol.
• Personnel Responsibilities: The roles of study directors, scientists, quality assurance personnel, and support staff are clearly defined.
• Test Systems and Facilities: Laboratories must maintain appropriate facilities and control systems for conducting studies. These include properly calibrated equipment, controlled environments, and standardized procedures.
• Data Integrity and Reporting: Data must be meticulously recorded, verified, and archived, ensuring it is traceable and reproducible.
• Study Documentation and Archiving: All records, raw data, and reports must be stored and accessible for a period defined by regulations, ensuring transparency and reproducibility.

• General considerations

 • Quality management principles

 • Objectives of 9001:2008

 1. Scope

 2. Normative reference

 3. Terms and definitions

 • Main clauses and sections of ISO 9001:2008

 4. Quality management system

 5. Management responsibility

 6. Resource management

 7. Product realization

 8. Measurement, analysis and Improvement

It has been well established that the risk of health problems rises as the amount of radiation exposure increases. All types of radiation produce changes in the living tissues. The resultant cellular injury causes physiological and pathological changes leading to “Radiation sickness”. The radiation effects may be somatic or genetic. Somatic effects are harmful to a person in his lifetime whereas genetic effects affect generations. Radiation may cause changes in complex molecular systems of living cells, primarily in following two ways :- 

A diagnostic radiograph is defined as one showing the tissues adequately penetrated, sharply outlined, and the variations in tissue opacity sufficiently demonstrated. An ideal diagnostic radiograph should have excellent detail, correct density and proper scale of contrast. The sharpness of the image determines the detail or definition of an image. There are two factors in radiographic quality control, viz: photographic and the geometric effect.  Density, contrast and detail are photographic aspects of radiographic quality. Detail and distortion are geometric aspects of radiographic quality. Because a radiograph is a two-dimensional (2-D) image of a three-dimensional (3-D) object, the appearance of a radiographic image varies with the patient’s orientation in relation to the primary X-ray beam. 

The radiographic appearance of various tissues is influenced by a number of factors which determine the character of X-rays.  Various exposure factors control the radiographic dentsity, detail and contrast: milliamperage (mA), time (sec), kilovoltage (kV), focal spot/film distance (FFD) and object-film distance (OFD), thickness and nature of the part radiogaphed, speed and type of film and intensifying screens, temperature and time of developing, grid type, incoming line voltage, make and type of X-ray machine. Of these, first five exposure factors (milliamperage (mA), time (sec), kilovoltage (kV), focal spot/film distance (FFD) and object-film distance (OFD) are very important as radiographic technique chart may be worked out by varying one or more of the five factors.  These exposure factors are integral to image quality and should, therefore, to be adjusted appropriately in order to achieve a diagnostic radiograph of optimal quality (image contrast, brightness and clarity).

Artifacts are any unwanted radiographic densities in the form of blemishes arising from improper handling x-ray film, exposure, processing, housekeeping or setting up a technique for an examination. Artifacts will reduce the overall quality of the radiograph and in certain cases may nullify its diagnostic outcome. An idea about the possible errors is desirable in order to adopt corrective measures.

One of the most important pieces of information in the field of  X-rays is a radiographic technique chart. If one select exposure factors from a good rating chart, consistent radiographic examinations of diagnostic quality will be obtained. In addition, there will be a saving of X-ray films because waste from repeated exposures will be avoided.

There are several types of techniques chart that can be formulated, each type must be formulated with the goat of maximum potential of a particular X-ray machine. Perhaps the most popular type of technique chart used by veterinarians is a variable kilovoltage technique chart. A variable mA second chart is probably more appropriate for the most powerful X-ray machine. However, combination of variable kilovoltage and mAs technique chart is best. Such charts take into consideration the need to adopt a technique chart for different body systems, such as a thoracic and abdominal study, as well as examination involving the musculoskeletal system. The principle of how to prepare a variable kilovoltage technique chart along with an example of such a chart is given.

Radiology has been an important diagnostic modality in exotic animals and birds (includes rodents, reptiles and fish). The radiographic equipment needed for radiography of exotic animals and birds is basically the same to that used for domestic animals. A high-milliamperage (mA) X-ray machine, having 200- or 300 mA unit is recommended to allow the use of a short exposure time (1/40 seconds) or less are preferred. Maximum kilovoltage (kVp, 40-75) is less important for avian and exotic radiography than for domestic animal radiography. As with other species, at least two radiographic views at 900 angles to each other are recommended. However, restraining and knowledge of radiographic anatomy of different species of exotic animals and birds are the prerequisite for the modality. Restrain and immobilization:

Defination

Dislocation of carpometarcarpus and tarso metatauses along with upper fracture of MC and MT bone (Fig. 1 and Fig.2). It is considered as difficult fracture and may not be healed appropriately by the usual procedure of lower level fracture repair method. Due to constant movement of fracture ends its treatment should be similar to middle level fracture repair method.

Materials Required

Thick padded cloth

Adhesive gum (fevicol)

Netted bamboo splint

Thomas splint

Recommended Duration for Slinging Large Animal

Aim: To know slinging duration for large animal.

Indication: Downer cow, sternal redumbency, hypocalcemia, hypomagnesemia, limb or spinal injury, cases,related to parturition.

The information in respect of reproductive parameters and events are recorded in respect of following points.
    
1.    Date of semen collection, evaluation, preservation and semen distribution from different bulls 
    
2.    Fertility status of semen 
    
3.    Reproductive performance of females 
    
4.    Work of inseminators