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Good quality semen is more acidic than semen with lower sperm concentration. In contrast, bacterial contaminated semen and semen with more dead spermatozoa evolve ammonia that will increase pH of semen. In orchitis, seminal vesiculitis and epididymitis the pH of semen will be alkaline due to presence of pus. In azoospermic or necrozoospermic semen, the pH may be neutral or alkaline. pH variation may be due to change in season/climate and frequency of successive ejaculates. Depending on the concentration and activity of spermatozoa, the pH of semen varies. The narrow range pH (BDH) paper are more convenient in routine work. The first change in color is matched with color on the cover of booklet. Use of BDH capillary set is time consuming whereas digital pH meters are also being used. The normal pH range is depicted in Table 11.1.

Semen is needed to be extended with a suitable medium and cryopreserved without affecting its survivability and fertility for the purpose of inseminating female artificially. Qualities of Ideal Extenders • Semen extender is a nutrient media which provide energy to spermatozoa, protection from osmotic shock, thermal shock and various metabolic wastes for prolonging the life span of sperm cell with maintaining the acceptable fertilizing capacity. A semen extender should have qualities to protect spermatozoa during dilution and cryopreservation. These qualities may be : 1. Preserving sperm cell integrity (membrane, cellular, mitochondrial and acrosomal integrity etc) 2. Maintenance of osmotic pressure (isotonic; 280-300 mOsm/l) similar to seminal plasma 3. Providing nutritive elements as energy source for sperm metabolism (aerobic & anaerobic metabolism)

• After equilibration, a pre-freeze evaluation of semen is done. Pre-freeze motility should be more than 70% at 370 C which makes the semen eligible for freezing. Semen Packaging • Semen is packaged in suitable container like pellets, glass ampoules, tupol (polyethene tubes) and semen straws. • In the Ampoule method, semen is packed in glass ampoules and stored in LN2 . Here labeling and handling is easier with less chances of contamination. But more storage space, more time taking to thaw and the use of glass catheter for insemination make their the use less convenient. • In Pellet methods, diluted semen is frozen by pouring it into the holes (0.1, 0.2 ml) made on dry ice (Solid CO2 at 790 c) and these pellets (frozen semen balls) are stored in LN2 . Here glycerolization is not needed as well as process is also quick but labeling is difficult and the chances to get contaminated are more. Handling pellets for insemination is also difficult (breakage or getting stuck to forceps during retrieval).

Etiology

It is an uncommon endocrine related skin disorder. It is very rarely encountered in neutered dogs.It is always associated with cryptorchidism and sertoli cell tumor. This condition is closely accompanied with endocrine related testicular tumor followed by skin lesion and feminization.

To set up of an X–ray (radiology) section following points should be considered while planning:
 
    1.    Anticipated present work load (number of cases and species of animals to be X-rayed) and future requirement in the next 10-15 years.
    2.    Financial resources available.
    3.    Type of equipment to be installed for radiographic work.
    4.    Requirement of radiation safety enforcing agencies e.g. of Bhaba Atomic Research Centre, Mumbai.
The overall set up of radiology section should consider the following requirements:

Most of ultrasound machines are designed for imaging with a particular resolution linear and convex transducers. These machines can be used for general purpose for abdominal, obstetrics, gynecological, small parts, urology and cardiac applications. Most of the machines have standard measurement software viz. distance, area, volume, circumference, and obstetrical gestational age measurement for BPD, HC, AC, FL, GS, CRL, AD and FW.

Assessment of probes: One should examine the probe whether it is linear array or curved (convex) array. The probe should also be checked for its frequency. These days multi-frequency probes are supplied with the ultrasound-machine. These multiple frequencies could be 2.0 to 5.0 MHz with centrally fixed frequency of 3.5 MHz. The frequencies could be 4.0 to 7.0 (5.0 MHz setting), 5.0 to 9.0 MHz (7.5 MHz setting). The area and depth covered by various frequencies should be known.

Before setting the ultrasound machine, one should make sure of all the auxiliary devices for the machines. These are Thermal video printer, Ultrasound gel, Biopsy needle, image saving devices (USB device or internal saving mechanism), wire, Uninterrupted Power Supply etc.

Identifying and knowing the main system: One should note down the name and model of ultrasound machine as these are quoted in all the documents published with the ultrascan images generated on that particular machine. It is written on one side of front panel. On the front panel, there are various knobs for proper setting. One should locate the auxiliary power switch of the machine to begin with. Generally, there is power indicator on the machine that shows light on when power button is pushed to on position. The auxiliary power switch may also have indications of its ON and OFF positions, may be marked with 0 and 1 or other symbols. As shown in the figure below, there are various operating buttons on the operating panel. These might vary from Machine to Machine.

Sexual behavior

The pattern of courtship, display, motor activities and postures are directed to bring the male and female gametes together to ensure fertilization and pregnancy. The components of copulatory patterns are sexual arousal, courtship (sexual display), erection, penile protrusion, mounting, intromission, ejaculation, dismounting and refractoriness. The duration of courtship and copulation varies with the species both events are shorter in cattle and sheep than in swine and horses. The males sniff and lick the genitalia of female are more common and an important way of chemical communication through olfaction. Except in swine, the males smell the female’s urine and then raise head, with lips curled, in the ritualized “Flehmen’s reaction”. In sheep, goat and cattle, tactile stimulation of the female is made by nuzzling and licking the perineal region, whereas with the horse, the stallion often bites the female’s neck and in swine, the boar noses her flanks.

• Sexual activity or mating behaviour is innate character of the males. Unlike the female animal, male animal has a nature of sexual receptivity permanently. • Male gonadal steroid androgen controls sexual behaviour. At regular interval of 2-4 hrs, there is secretion of testosterone which converts into estradiol due to aromatization. Due to this very frequent rise in estradiol level, male shows sexual behaviour permanently. In female animals, a rise in estradiol occurs only during estrous. • When in a group of animals, any female can be mated with any male, it is called promiscuous sexual behaviour. Sexual behaviour in some animals is according to their social structure and territorial/home range i.e. male and female share some of their home range for the purpose of mating e.g. Roe deer, Muntjak antelope etc. In some animals, both males and females share whole of their territory for the mating purpose called a harem e.g. wild rabbit. Some animals come into a temporary territory meant only for mating in breeding season e.g. seal.

Shock is a life threatening condition which is usually manifested by serious pathophysiological abnormalities. In most cases it is due to poor tissue perfusion with impaired cellular metabolism.

       It is a clinical state resulting from an inadequate supply of oxygen to the tissues or an ability of the tissues to properly use oxygen.

       Shock can also be defined as imbalance between oxygen supply and oxygen consumption. In other words, the delivary of oxygen does not meet the needs of the tissues.

       Shock is a state in which the vital functions of the body are depressed because of acute reduction in cardiac output and effective circulating blood volume.

Key Points

• Shock occurs when the circulatory system fails to provide adequate blood flow to essential organs.

• Oxygen deficiency is a clinical condition caused by insufficient oxygen delivery or tissue incapacity to use oxygen effectively.

• Successful shock management requires early recognition, aggressive monitoring, and appropriate interventions.

Introduction

Shock is a complex physiological syndrome marked by widespread circulatory irregularities that result in inadequate tissue perfusion. In other words, it is a clinical condition caused by an insufficient delivery of oxygen to tissues or the tissues' inability to correctly use oxygen. It is a complication of trauma and diseases typically seen in emergency rooms, such as heart failure, inflammatory conditions (e.g., pancreatitis), and sepsis. In shock, tissue perfusion and oxygen delivery are insufficient to support the tissues' baseline metabolic demands. Whatever the cause, shock is distinguished by reduced blood flow, which is typically accompanied by low blood pressure (hypotension).

Indication

Irreparable injury.

Frost bite, burn.

Malignant neoplasia.

Incurable peripheral vascular disease.

Necrosis, gangrene, extensive injury of nerve of fore limb.

There are countless biomarkers present in cells related to and determining the progress of a disease. Identifying these markers could aid in detecting cells with metastatic potential (Ramaswamy et al., 2003).

The elevation of cyclo-oxygenase (Cox-2) expression was observed in CMT cases through enzyme immunoassay (Queiroga et al., 2005). In further study, enhanced expression of BRAC1and BRAC2 genes, certain enzymes such as matrix metalloproteinases (MMP-2 and MMP-9) by immunohistochemical examinations, and Bcl-2 & Bcl-XL elevated level in dogs with mammary neoplasm have been reported by Queiroga et al. (2011b).

Orthopaedic Radiography
 
Radiography as an aid to diagnosis the orthopaedic cases has become an integral part of every modern hospital. This technique may also be helpful in detecting some previously unrecognized lesions in the area radiographed.

The radiologist must be aware of the radiological anatomy of part being examined, and of its normal variation with age, species and breed. The anatomical features which can be appreciated in a radiograph are:

Objetive

To completely exercise a cancerous hoof in presence with cauliflower like growth with regular bleeding & myosis.

Material Required

Amputation saw, thick padded with bandage roll, a mixture of powder containing resin and naphthalene ball powder, bicycle tube protection sleeper (Fig-3), local anesthesia.

1. Tubercoxae
2. Twelth thoracic vertebra
3. First lumbar vertebra
4. Last rib
5. First coccygeal vertebra
6. Sacrum
7. Tuber ischii
8. T rochanter major
9. Femur
10. Lateral condyle of Tibia
11. Tibia
12. Distal end of fibula
13. Tuber calcis
14. Tarsus
15. Metatarsus
16. Phalanges
17. Patella

The condition in the young subject is as follows:

The two parietals are united with each other and also with the interparietal and supraoccipital. The resulting mass is somewhat horse shoe shaped. Its occipital part (planum occipitale) forms the greater part of the posterior wall of the cranium and bears about its centre the tubersity for the attachment of the ligamentum nuche. From either side of this a line curves outward and divide the surface into an upper smooth area and a lower area which is rough for muscular technique. The upper borderjoins the frontal bone and concurs In the formation of the frontal eminence. The temperal part (Plana temporalla) are much smaller and are concave externally; they join the frontal above and the squamous temporal below. A median occipital crest extends ventrally from the external occipital protuberance.

Foramen magnum

Occipital condyle

Paramastoid process

Condyloid foramen

Foramen lacerum

Basilar part of occipital condyle

Basilar tubercles

Bulla tympanica

Foramen ovale(concealed by muscular process)

Meatus acusticus externus