eChapters

• General considerations

 • Blood glucose

• Total serum protein

 • Serum albumin

 • Serum globulin

 • Serum gamma globulin

 • Serum calcium

 • Serum inorganic phosphorus

 • Serum magnesium

 • Serum cholesterol

 • Serum creatinine

 • Serum urea

 • Serum chloride

 • Serum sodium

 • Serum potassium

 • Serum Glutamic Oxaloacetic Transaminase

 • Serum Glutamic Pyruvic Transaminase

 • Serum alkaline phosphatase

Only few single sperm viability parameters show a significant correlation with fertility of semen samples. Therefore, need to explore functional in vitro fertility tests having capability to disclose the ability of spermatozoa to undergo complicated processes such as capacitation, binding of zona pellucida, acrosome reaction, fertilization of Oocyte and induction of embryo development invitro have been designed and explored for their correlation with fertility achieved after artificial insemination.

In vitro fertility tests used in evaluation of semen includes: 

14.1 Estimation of membrane integrity
        
    14.1.1 Morphological integrity
        
    14.1.2 Functional integrity
        
    14.1.2.1 Hypoosmotic swelling test
        
    14.1.2.2 Acrosome integrity

14.2     Cervical Mucus Penetration Test

• General considerations

 • Polyacrylamide gel electrophoresis (PAGE)

 • SDS-polyacrylamide gel electrophoresis (SDS PAGE)

• Nucleic acid hybridization

 • Radio labelled probes

 • Biotin labelled probes

 • DNA finger printing

 • Polymerase chain reaction (PCR)

 • Reverse transcriptase polymerase chain reaction (RT PCR)

Introduction
A study protocol tool is a very important comprehensive document that deliberately outlines the whole research rationale design and plan for the successful execution of the epidemiological/ an experimental/ a clinical study. It serves as a blueprint for how the study will be conducted, ensuring consistency, transparency, and adherence to ethical guidelines. The document contains the background information available in literatures on the basis of which the researchers plan to conduct the study with objectives that will answer research related questions with ensuring adherence to ethical standards.
Basic Elements of a Study Protocol
The study protocol includes different components including background Information available in literature for research plan, objectives or purpose of conducting the research, explanation of the study design, selection and exclusion of subjects, treatment of subjects, assessment of outcome safety and efficacy. The purpose of conducting research includes questions like why the study needs to be done and what will be its relevance to scientific society or environment. To achieve these objectives the researchers, design their research work for proper execution of research objectives. The study which is to be designed has structured framework for researchers to answer their research questions and obtain reliable and valid results. The study design not only helps the researcher in learning technical skills but also discovering the creative voice (planning and execution). The study design includes different methods, processes and necessary resources for accomplishment of research problems. The study design also incorporates the methods of data collection and statistical analysis which meets the requirements of desired outcome. The implementation of an appropriate study design guarantees a methodical and exacting research process, resulting in more precise findings and the appropriate type of analysis to address the research-related queries. This makes the conclusions more reliable and legitimate. By ensuring that the data are reliable, repeatable, and validated, a well-designed study contributes to the advancement of knowledge. Additionally, it helps to control biases and confounding variables that could affect the results and helps to clarify the methodology of the study, which

A neurological examination is an advanced and specialized clinical examination that is performed when there is suspicion of potential nervous system involvement. Before performing neurological examination a detailed history is essential. Neurological examination along with history not only helps in confirming whether the neurological problem is of primary or secondary origin but also aids in accurately identifying the location of the lesion. Common Terminology used in Neurology The following terms are commonly used in neurology. For a better understanding, common neurological terms with their definition are given below: •     Ataxia - An abnormal gait marked by lack of coordination, but without spasticity, weakness, or involuntary movements. Ataxia results from damage to the proprioceptive pathways, the vestibular system, or the cerebellum.

A variety of glassware like burettes, pipettes, measuring cylinders, flasks, separating funnel etc. are used for experimental purposes in a laboratory. All of these need to be used and handled correctly, otherwise the chances of experimental error and damage to the glassware increase significantly. Being made of glass, they need special care while washing and using in experiments because even a minor mistake in handling of glassware may result in breakage of the apparatus. With this precautionary note, let us discuss about some of the specific glassware used in the laboratories.

Burette

Burette is a long graduated glass tube with a stopcock at one end for delivering a known/desired volume of a liquid, especially in titration experiments. It contains a stopcock at the lower end and a tapered capillary tube as the stopcock’s outlet. The flow rate of liquid from the tub stopcock’s outlet can be controlled with the help of stopcock valve. Burette is used for measuring the volume of a solution/chemical added in a quantitative (titrimetric) estimation experiment.

Procedure of using a Burette

The burette reading must be noted before and after delivering the chemical/ solution from the burette. The difference in these two readings is the volume of the chemical/solution released out of the burette. The solution should be released slowly/drop-by-drop to avoid any experimental error/mistake. If the solution/chemical is allowed to pour out too fast, the wall of the burette will not drain properly and some liquid may remain sticking to the surface of the walls. Moreover, excessive amount of chemical may be released/dropped during the experiment, particularly during the titration experiment wherein we need to know the exact amount of acid/base needed to get neutralization point. Thus, any minor mistake may lead to get a faulty reading. The measuring capacity of a burette usually used in a laboratory is 50 mL.

1. Bone grafts

Bone grafting represents one of the earliest devised reconstructive approaches to the musculoskeletal system and remains among the most commonly used orthopedic procedures. Bone is the second most frequently transplanted tissue. The first recorded attempt to use bone graft is by the Dutch surgeon Job Van Meek’ren in 1668.

Definition

A graft is a living tissue that is transplanted to another site either within the same individual or into another individual for repair of a defect. An implant is a non viable material placed in a living system. Implants are composed of bone cement, metal or ceramic material. Grafts are structurally important for mechanical stability, but their use evolved to include the stimulation and enhancement of bone in response to injury.

Indications

Bone grafts are used, in general as framework, both to provide stability and to augment healing. It is used in the management of non union, delayed union, treatment of pseudoarthrosis, filling of osseous defects or cavities, arthrodesis, stabilization of spinal segments and addition of bone stock in total joint displacement.

Repairing fractures in small animal’s presence challenges to the veterinarians in their day to day service. These challenges include properly repairing fractures, each of which is unique, choosing the right fixation device, keeping an extensive inventory of costly instruments, and providing high standards of care for fracture repair. But fracture repair also gives veterinarians the opportunities to do something a little unusual in daily practice and to experience the immediate gratification of easing pain and promoting activity in injured animals.

Successful management of fractures mainly depends upon early diagnosis, type of fracture, timing of surgery and selection of proper method of fixation. The fixation of fractures can be achieved either by external or internal fixation methods. An ideal method of fracture fixation is one that can maintain early anatomical reduction, minimal soft tissue trauma, rigid stability and early motion of bone and joint. Restoring a fractured bone’s normal shape is important for return to function because it contributes to normal alignment of adjacent joint surfaces. Whereas, rigid stability of fracture fixation eliminates fragment movement and reduces pain. Soft tissue trauma adjacent to fractured bone should be minimum, which play a vital role in early vascularisation and prevents infection. Finally, early return to function maintains muscular tone of the limb allows normal weight mobility and joint nutrition and uses normal weight bearing forces to maintain bone density. These can be achieved with use of internal fixation devices.

Collections of blood To collect blood for different haematological analyses, an animal’s vein are punctured with a syringe and needle. Requirements • Hypodermic needle • Syringe • Collection vials or tubes containing anticoagulantsProcedure • The place of blood collection should always be cleaned with alcohol and hairs trimmed. • Press on the vein to raise it. • Free-flowing blood can be collected directly into the vial containing anticoagulant after the needle is inserted into the vein. • 5 ml of blood from large animals and 2.0 ml of blood from small animals like dogs and cats are sufficient for normal haematological investigations. • Rotational motions in the vials are used to mix the blood

8.1. Classification

The ruminants belong to the Order Artiodactyla. The families in order of Artiodactyla include Camelidae, Tragulidae, Cervidae, Giraffidae, Antliocapridae, and Bovidae.

The family Camelidae includesGuanaco (Lama guanicoe), Llama (Lama glama), Alpaca or South American camelid mammal (Lama pacos), Dromedary camel (Camelus dromedarius), and Bactrian or two-humped camels (Camelus bactrianus).

The family Tragulidae includes Chevrotains originate from Africa (Hyumo schusaquaticus) and Chevrotains that originate from Asia (Tragulus species).

The family CervidaeincludesWhite tailed deer(Odococlius virginianus), Follow deer (Dama dama), Roe-deer (Capreolus capreolus), Axis deer (Axis axis), Reindeer (Rangiter tarandus), Musk deer (Moschus species) and Chinese water deer (Hydropotes species).

9.1. Classification: Order Perissodactyla

The order Perissodactyla, also known as odd-toed ungulates, is a group of herbivorous mammals characterized by having one or three toes on each foot. They are distinguished from even-toed ungulates (Artiodactyla) by this anatomical feature. Even-toed ungulates, such as pigs, cows, and deer, have an even number of toes (two or four) on each foot. Some of the well-known members of this order include horses, zebras, asses, tapirs, and rhinoceroses.

The name “Perissodactyla” comes from the Greek words “perissos,” meaning “odd,” and “daktylos,” meaning “finger or toe,” referring to their unique foot structure. Unlike even-toed ungulates (Artiodactyla), which bear weight on their third and fourth toes, perissodactyls primarily bear weight on their third toe.

Introduction
Ageing, a multifaceted and intricate process, is characterized by a series of transformations that occur at various levels of biological hierarchy, ultimately culminating in death. This phenomenon, marked by its complexity, involves numerous factors that interact simultaneously and operate across different levels of functional organization. The diversity of ageing phenotypes among individuals of the same species and the disparities in longevity
among different species underscore the significant roles of both genetic and environmental influences in shaping the lifespan.At its core, ageing is an ontogenic journey—a process of growing old that encompasses all physiological, genetic, and molecular changes occurring from fertilization to death. The biology of ageing pertains to the progressive accumulation of random molecular defects within tissues and cells, leading to age-related functional impairments in tissues and organs. This relentless, deleterious, and intrinsic phenomenon was famously described by Medawar in 1952 as an “unsolved problem in biology.” Despite the enduring mystery of the ageing process, research has advanced significantly since Medawar’s time, expanding our understanding of this intricate biological conundrum.
Goldberger et al. (2002) define senescence as the progressive deterioration of bodily functions over time, noting that normal human ageing is associated with a loss of complexity in various physiological processes and anatomical structures. Although ageing and senescence are often used interchangeably, they encompass distinct concepts. Senescence refers to a post-maturational process that leads to diminished homeostasis and increased vulnerability to death, while ageing encompasses any time-related process that begins at conception and continues until death. The mechanisms driving ageing are partially intrinsic, influenced by genetic and epigenetic factors, and partially extrinsic, shaped by environmental factors such as nutrition, radiation, temperature, and stress. Genetic factors account for approximately 25% of the variance in human lifespan, with the remainder determined by nutritional and environmental influences. 

Introduction
Eye
Visual perception, eyesight, sight, or vision is the ability to interpret the surrounding environment using light in the visible spectrum reflected by the objects in the environment. The eyes are the sensory organs that are extension of the brain; they receive visual information from the environment and transmit them to the visual sensory area of the brain for interpretation.
The pineal and para-pineal glands are photoreceptive in non-mammalian vertebrates. Birds have photoactive cerebrospinal fluid (CSF)-contacting neurons within the paraventricular organ that respond to light in the absence of input from the eyes or neurotransmitters.
Accessory Structure of the Eye
Eyelids - (Palpebrae) The free margins of the eyelids form the palpebral fissure. The edges where the upper and lower eyelids meet are called the lateral and medial canthus. The palpebral margins are lined by eyelashes. The eyelids are supported internally by sheets of connective tissue that form the tarsal plate.
Glands associated with the eyelids
Glands of Zeis - These are sebaceous glands associated with the eyelashes.
Tarsal glands - (Meibomian glands) - These glands line the inner margin of the lid and
produce a lipid-rich product that prevents the lids from sticking together.
Lacrimal caruncle - This is a mound of tissue in the medial canthus that produces thick secretions.
Conjunctiva It is the epithelium that lines the inner surface of the eyelids and continueson to the outer surface of the eye.
Palpebral conjunctiva lines the inner surface of the eyelid.
Bulbar conjunctiva lines the anterior surface of the eye.
Lacrimal Apparatus It produces, distributes and removes tears. Tears reduce friction, remove debris, prevent bacterial infection and provide nutrients and oxygen for the conjunctiva. Lacrimal glands are located near the lateral canthus of the eye. Tears flow over the cornea and are drained into the nose by the nasolacrimal duct.

Luxation/Dislocation : It refers to the displacement of opposing articular surfaces of bone. The direction of displacement is described with reference to the distal bone in relation to the proximal bone.

Subluxation : It refers to partial or incomplete dislocation.

Classification
    1.    Acute : Recent occurrence of luxation.
    2.    Chronic : Luxation existing for a long time.
    3.    Recurrent : Reluxation after correction.
    4.    Simple : No open wound is communicating with the joint.
    5.    Compound : Open wound is communicating with the joint.
    6.    Pathological : Dislocation due to any pathological lesion.

Introduction
The haematological system, essential for sustaining life and comprising blood and its components, is fundamental to human and animal physiology. Blood is a unique connective tissue that performs several critical functions, including transporting oxygen, nutrients, and hormones to cells and removing metabolic wastes. It also plays a pivotal role in immune response, haemostasis, and the regulation of body temperature and pH. This
section provides an in-depth overview of the clinical physiology of the haematological system, covering the composition of blood, haematopoiesis, haemostasis, and common haematological disorders. Blood is composed of two main elements: plasma and blood cells. Plasma, the liquid component, constitutes approximately 55% of blood volume and consists predominantly of water, electrolytes, proteins, waste products, and dissolved gases. Plasma is the medium for transporting nutrients, gases, hormones, and waste products throughout the body. It contains important proteins such as albumin, which maintains osmotic pressure; globulins, which are involved in immune responses; and fibrinogen, which is crucial for blood clotting. The blood cells include erythrocytes (red blood cells), leukocytes (white blood cells), and platelets. Erythrocytes are the most abundant cells in the blood, with the primary function of transporting oxygen from the lungs to tissues and carbon dioxide from tissues back to the lungs. This is facilitated by haemoglobin, an iron-containing protein within erythrocytes that binds to oxygen. The lifespan of an erythrocyte is approximately 120 days, after which it is phagocytosed by macrophages, which are a type of myeloid phagocytic cells in the spleen and liver. Leukocytes are integral to the immune system, protecting the body against infections and foreign pathogens. They are categorized into granulocytes (neutrophils, eosinophils, and basophils) and agranulocytes (lymphocytes and monocytes).

Artificial intelligence offers practical solutions to agricultural problems associated with population growth, food demand, climate change and limited natural resources. This chapter introduces machine learning, deep learning, computer vision and natural language processing, together with enabling technologies such as IoT, remote sensing and cloud computing. It explains their applications in crop monitoring, disease recognition, yield forecasting, irrigation, soil assessment and livestock management. AI can improve decision-making, resource efficiency, automation and precision farming while reducing reliance on manual labour. The chapter also examines adoption barriers, including high costs, limited digital literacy, data-security concerns and weak rural infrastructure, and considers future developments in autonomous and climate-resilient farming.

Injectable Anesthesia

Injectable anesthetics are the agents which are administered to the patient parentrally. They include drugs which are given intravenously to rapidly induce anesthesia in a patient. Single or combinations of drugs usually administered intramuscularly to patients which are difficult to handle. Injectable agents generally require less equipment and are easier to administer than inhalation anesthetics, but it must be remembered that once they are given to a patient they cannot be removed.

Artificial intelligence can detect subtle changes in animal health before they become apparent through conventional observation. This chapter examines machine learning, deep learning and predictive analytics for livestock disease prediction and diagnosis. Models use physiological signals, diagnostic images, behaviour patterns, environmental conditions and historical records to recognize abnormalities and estimate disease risk. Wearable sensors and IoT devices enable continuous monitoring and early-warning systems, supporting preventive veterinary care and outbreak management. AI-based detection can reduce mortality, treatment costs and production losses while improving decision-making. The chapter also evaluates challenges involving inconsistent data, model interpretability, rural infrastructure, technical accessibility and the practical implementation of diagnostic systems.

Animal behaviour provides valuable information about health, welfare, reproduction, environmental adaptation and social interaction. Traditional observation is labour-intensive, time-consuming and vulnerable to observer bias. This chapter explains how machine learning automates behavioural analysis using video, sensor and bioacoustic data. It covers preprocessing, feature extraction, classification, clustering and recurrent neural networks for temporal analysis. Computer-vision techniques—including object detection, tracking and pose estimation—enable continuous and non-invasive monitoring. Case studies demonstrate applications in livestock management, wildlife conservation and laboratory research. The chapter also examines data labelling, model generalization, ethics, explainable AI, real-time processing and the integration of multiple behavioural data sources