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In GADVASU, under the DBT sponsored project on “Development and evaluation of serodiagnostic assay for timely diagnosis and prognosis of mammary tumors”, we have generated and used hyper-immune sera raised against certain canine recombinant mammary tumor associated proteins for the standardization of sandwich and indirect ELISA for serodiagnosis of canine mammary tumor. The sensitivity and specificities of the assay’s ranges between 85-88% and 80-85%, respectively. However, an antibody against a single marker when used for different histotypes of CMT, its specificity reduced significantly. Multiplex assays are capable to detect many specific antigens in a single run whereas a traditional ELISA is used only to detect a single antigen. It has high-throughput potential, providing more results per sample, with lower sample volumes and with utmost sensitivity and specificity. Attempts are being made to increase the sensitivity and specificities of the assays by multiplexing various markers together (Fig.4).

A wound is defined as a breach or disruption in the normal continuity of tissue in any body part. The wounds are mainly caused by physical, chemical or biological insult.

Key Points

• Provide immediate first-aid for wounds.

• For bite wounds, open healing is preferable.

• Silver nanoparticles have antibacterial properties and have been investigated for anticancer applications.

Introduction

Cuts, tears, burns, fractures, and other forms of live tissue injury are all considered wounds. Wounds are typically classified as clean, contaminated, or diseased. Clean wounds are ones that occur in sterile settings, such as surgical incisions. The quantity of germs present distinguishes between contaminated and infected wounds.

Initial Wound Management

The initial step in wound management should be to assess the animal's general stability. Obvious open wounds can divert attention away from more subtle but possibly fatal conditions (such as shock). The general health of the animal influences all areas of care and healing. Some diseases, such as anemia, may impair wound healing by lowering oxygen levels. Malnutrition may also disrupt the healing process. After the initial assessment, the patient should be stable.

The X-ray film is the major element that record the image of part exposed with X-rays. Although the situation is changing with the introduction of new technologies in recent years. The film can be exposed by the direct action of X-rays, but more commonly the X-ray energy is converted into light by intensifying screens and this light is used to expose the film. The X-ray film is somewhat similar to photographic film in its basic composition. However unlike photographic film, the light (or radiation) sensitive emulsion is usually coated on both sides of the base of X- ray film so that it can be used with intensifying screens. Because of its importance to medicine, X-ray film is manufactured with consistent uniformity and quality, which facilitates standardization of exposure and processing.

    1.    X-ray beam collimators: Comprised of 2 independently-acting sets of adjustable lead shutters. These are the devices made of lead and are used to restrict X-ray beam up to a desire area. In diagnostic radiology they are essential for safety of the patients, and persons and also to prevent fogging of films due to unnecessary radiations. The collimator is the most effective of beam limiting devices.

A tube rating chart is a guide regarding operational limits of the X-ray tube for single and multiple exposures and permissible heat load of the anode and the tube housing. It is an essential component for obtaining diagnostic X-ray examinations is a consistent way.
 
The tube rating chart provides important information on the maximum safe exposure time that can be used with specific mA and kV setting. If lower than designated exposure times are used, tube damage may occur. The size of the anode focal spot determines the rating of the tube because size controls the amount of energy it can absorbs and convert into X-rays and heat. Therefore, one should never use an X-ray chart formulated for another X-ray machine (even those made by the same manufacture) without making appropriate changes. Knowledge of three charts is important to extend tube life and includes tube rating chart, anode heat cooling chart and housing cooling chart.

    ·    An X-ray tube is an energy converter. It receives electrical energy and converts it into two other forms: X-radiation and heat.

    ·    It is a source of X-rays used in diagnostic radiology.

    ·    Designed and constructed to maximize X-ray production and to dissipate heat as rapidly as possible.

    ·    It is a thermo ionic diode type electronic vacuum tube where X-rays are produced.
 
    ·    A basic element of an X-ray tube (Figure) consists of the cathode, anode, glass tube and tube housing.