eChapters

Introduction
Antimicrobial resistance (AMR) is increasingly recognised as a “silent pandemic,” threatening global health and development. In 2019 alone, bacterial AMR was directly responsible for 1.27 million deaths and contributed to nearly 5 million more worldwide. Projections warn that by 2050, unchecked AMR could result in 10 million annual deaths and impose a staggering economic burden of USD 100 trillion. A major driver of this crisis is the indiscriminate and often prophylactic use of antibiotics in livestock production systems, which account for a majority of global antimicrobial consumption. The One Health framework—emphasising the interconnectedness of animal, human, and environmental health—offers a strategic pathway for mitigating AMR. It requires dismantling sectoral silos and fostering collaboration among veterinarians, physicians, public health authorities, food safety regulators, and environmental scientists. Against this backdrop, NDDB has positioned itself as a frontrunner in India by embedding One Health principles into large-scale field programmes. Since 2014, NDDB has progressively promoted alternative medicine for bovine mastitis control under its Mastitis Conrol Project (MCP), subsequently expanding in 2017–18 into a broader Disease Control through Alternate Methods (DCAM) project with Ethnoveterinary Medicine (EVM) as the central pillar.
Building Up: From DCAM to DISHA as One Health Strategy
Building on the successes of MCP, DCAM, and BCP, NDDB launched the Dairy Integrated Safety and Health Action (DISHA) project for 2025–2030, DISHA consolidates NDDB’s One Health strategy by combining animal health management, zoonotic disease surveillance, and food safety monitoring. Its objectives include: managing bovine cases with EVM, organising farmer awareness programmes, training veterinarians and health workers, screening cattle as well as their owner for brucellosis, and testing bulk milk samples for aflatoxins and antibiotic residues. Importantly, DISHA also addresses emerging food system contaminants such as microplastics, per- and polyfluoroalkyl substances (PFAs), Bisphenol-A (BPA) and heavy metals, thereby broadening the scope of One Health beyond infectious diseases.

Hazard Analysis Critical Control Points (HACCP) is an internationally recognized method of identifying and managing food safety related risk and, when central to an active food safety program, can provide customers, the public and regulatory agencies assurance that a food safety program is well managed. HACCP is a management system in which food safety is addressed through the analysis and control of biological, chemical, and physical hazards from raw material production, procurement and handling, to manufacturing, distribution and consumption of the finished product. (FAO, 2003)

Mycotoxin hazard analysis and identification of possible control measures

I. Hazard Analysis

a) Identification of mycotoxin hazard

For a given commodity system in a particular location, the HACCP team need to first consider which, if any, of the mycotoxins known to constitute a food safety hazard are likely to be present. Over 300 mycotoxins are known, but only a relatively few of these are widely accepted as presenting a significant food or animal feed safety risk.

The following mycotoxins have regulatory limits set by one or more countries: the aflatoxins (including aflatoxin M₁), ochratoxin A, zearalenone, patulin, ergot alkaloids, and deoxynivalenol. Guideline limits exist for fumonisin B₁ and regulatory limits are likely to be set in the near future. The regulatory limits are taken as the target levels and should be included in the Product Description table. Mycotoxin limits can also be set by the customer in specific contracts and it is possible that these may include mycotoxins not subject to regulatory limits.

The main purpose of the immune system is to protect the body from foreign substances, diseases or infections. When the body is exposed to a microbe or a foreign substance (antigen), the body makes some response which is called immune response. The purpose of immune response is to recognize, neutralize and eliminate the antigens from the body. Generally, the term immunogen is also used for antigen. Both, antigen and immunogen are used as synonyms but specifically, they are different from each other.

Obviously human have had to deal with microbes even before the recorded history. The first record of human using microorganisms comes from ancient tablets from mid east.

Babylonians were using yeast to make beer over 8000 years ago and acetic acid bacteria to make vinegar over 6000 years ago. About 5000 years ago, Persia (Now Iran) region recorded the wine making.

The Romans had God for specific microorganisms. The roman God of mold and mildew were “Robigus” and “Robigo” which mean crop rust. (Rust is one of the plant disease caused by fungus). God Robigus was very much feared because of crop lost. About 2000 years ago, Romans proposed that diseases were caused by tiny animals. But, fundamentalist religions had a strong hold over the progress. The real microbiology history starts from 1600s, when people began to make crude lenses and microscopes.

The following are the scientists and their role in the development of microbiology:

Proto – first; Zoon – animal

Protozoa are single celled eukaryotic organisms, which lack cell wall and ability to move at some stage of their life cycle.

They are referred as the first animals.

There are about 65000 described species grouped into 7 phyla. Among them, 50 per cent are not available as living forms (as fossils) and out of remaining 50%, 22,000 species are free-living and 10,000 species are parasitic forms.

The protozoa are distinguished from other organisms by following characters

Sterilization refers the complete removal or elimination of microorganisms from an environment. Physical and chemical agents are available to remove the microorganisms from the environment. Heat, irradiation and filtration are the three physical agents and various chemicals are available for sterilization.

Terms related to control of microorganisms

Cide or cidal means to kill the microorganism (Ex. Bactericidal refers the killing of bacteria).

Static or stasis means inactivation or inhibition, but not killing. They prevent multiplication of the organism. (Ex. Fungistatic refers the inhibition of growth of fungi.).

Sepsis refers break down of a living tissue by organisms and is accompanied by inflammation and pus formation.

Antispetic is an agent applied externally on living tissues to kill or inhibit the growth of the organisms.

Disinfectant is an agent applied externally on inanimate objects (not living tissues) to destroy harmful pathogens in their vegetative stage.

(Antiseptics are milder than disinfectants because to avoid the side effects)

Sanitation refers removal of organisms from a location by cleaning but not by sterilization.

The fungal exometabolome represents the collection of metabolites secreted by fungi into their surrounding extracellular environment. This chapter introduces the concept of the fungal exometabolome and distinguishes it from the intracellular endometabolome. It discusses the composition of extracellular metabolites, factors influencing their production, methods used for exometabolome analysis, and their biological functions. Fungal exometabolites can contribute to communication, nutrient acquisition, competition, host interactions, and environmental adaptation. The chapter also examines industrial and medical applications and the role of fungal exometabolomics in systems biology. Advantages, limitations, analytical challenges, and future perspectives are discussed to demonstrate the value of extracellular metabolite analysis in fungal research.

WATER IS VERY SPECIAL IN BIOLOGY

Most abundant chemical of life

Special properties:

I. WALL COMPONENTS – CHEMISTRY

The main ingredient in cell walls are polysaccharides (or complex carbohydrates or complex sugars) which are built from monosaccharides (or simple sugars). Eleven sugars are common in these polysaccharides including like glucose and galactose. Carbohydrates are good building blocks because they can produce a nearly infinite variety of structures. There are a variety of other components in the wall including protein, and lignin. Let’s look at these wall components in more detail:

A. Cellulose: β1,4-glucan (structure provided in class). Made of as many as 25,000 individual glucose molecules. Every other molecule (called residues) is “upside down”. Cellobiose (glucose-glucose disaccharide) is the basic building block. Cellulose readily forms hydrogen bonds with itself (intra-molecular H-bonds) and with other cellulose chains (inter-molecular H-bonds). A cellulose chain will form hydrogen bonds with about 36 other chains to yield a microfibril. This is somewhat analogous to the formation of a thick rope from thin fibers. Microfibrils are 5-12 nm wide and give the wall strength - they have a tensile strength equivalent to steel. Some regions of the microfibrils are highly crystalline while others are more “amorphous”.

ALL MOLECULES MOVE CONTINUOUSLY BY SIMPLE DIFFUSION

Heat energy causes molecules to move randomly (sometimes called Brownian motion)

If the concentration of molecules is different in 2 regions (this produces a concentration gradient), diffusion will cause molecules to move from a region of high concentration to one of low concentration

The higher the concentration gradient the more rapid the net diffusion

Diffusion evens out the concentrations so they are equal everywhere (maximum entropy)

If 2 different substances have the same concentration gradient, usually one will move faster than the other

Differences in speed between molecules with the same concentration gradient are given in terms of diffusion constants

A substance with a high diffusion constant moves faster than one with a low diffusion constant

In general, large molecules move slower than small molecules

Diffusion across a membrane is called permeability, and membrane diffusion constants are called permeability constants

Guillame de Baillou (1673), a physician, described an outbreak of recurrent severe cough that could induce vomition and suffocation in human in Paris. The infection was described as ‘Quintan^tussis which was later confirmed as ‘whooping cough’. The causative agent Bordetella pertussis was discovered by Jules Bordet and Octave Gengou in 1906 in Paris. The Gram-negative bacilli were isolated from sputum of Jules Bordet’s son. Bordetella bronchiseptica was first isolated by Ferry (1911) and M’Gowan (1911) who considered the bacteria as the cause of distemper in dogs. Later B. bronchiseptica was known as primary cause of ‘kennel cough’ infection along with the Canine Adenovirus and Canine Parainfluenza virus. Moreno- Lopez named the bacteria B. bronchiseptica.

The genus Moraxella was named after Morax who first isolated the bacteria from human conjunctivitis in 1896.

Algae are a large and diverse group of eukaryotic organisms that contain chlorophyll and carry out the oxygenic photosynthesis.

Study of algae is referred as phycology or algalogy

They vary from cyanobacteria or blue green algae by their eukaryotic nature.

Introduction

Mankind has been utilizing different animal species from the dawn of civilization for a variety of purposes viz., production of milk, meat, wool, egg and leather. Apart from these, various animal species are also used for draught power, companionship, entertainment, research experimentation, sports, security etc. Livestock sector plays a crucial role in rural economy and livelihood. As per report of the working group on animal husbandry and dairying, 11th five year plan: 2007-12, the livestock sector employs eight percent of the countries labour force, including many small and marginal farmers, women and landless agricultural workers. Milk production alone involves more than 30 million small producers, each raising one or two cows or buffaloes. The organic fertilizer produced by the sector is an important input to crop production and dung from livestock is widely used as fuel in rural areas. Livestock also serves as an insurance substitute, especially for poor rural households; it can easily be sold during time of distress.

The livestock play a significant role in generating gainful employment in rural areas, particularly among the landless and marginal farmers. The Jammu region of state of Jammu and Kashmir possesses a huge livestock population which is maintained under different management systems. The region has a vast diversity in agro-climatic conditions (comprising low altitude, middle altitude and high altitude), and animal husbandry and pasture management practices. Despite having huge livestock, the production potentials are very low. There are various factors related with low production and among them parasitic diseases rank very high.

The polymerase chain reaction (PCR) is a rapid way of amplifying (duplicating) specific DNA sequences

Method was devised by Kary Mullis of Cetus Corporation, Emeryville He recieved a $20,000 bonus and later a Nobel Prize

DNA heated to high temperature is not destroyed; separates into single strands, but reforms helix when cooled

PCR Method:

DNA to be amplified is put into solution containing:

WHAT IS PLANT?

A. Definition - by most definitions, a plant:

is multi-cellular;

is non-motile

has eukaryotic cells

has cell walls comprised of cellulose

is autotrophic; and

exhibits alternation of generations - has a distinctive diploid (sporophyte) and haploid (gametophyte) phase.

The term growth for bacteria and other microorganism usually refers to changes in the total population rather than an increase in size or mass of an individual organism. As the mechanism of conservation and dissipation of heat generated during metabolism lacks in bacteria, the enzyme system in bacteria gets directly influenced by temperature. The purpose of studying the bacterial growth curve is a) to become familiar with the population growth dynamics of bacterial culture and b) to plot a growth curve and determine the generation time of bacterial culture.

A number of methods are now available for measuring bacterial growth. Some important ones are

1)By colony counting or cell counting

2)By weighing the cells

3)By cell activity (turbidity method)

Antibiotics are produced by some true bacteria, actinomycetes and fungi that destroy or inhibit the growth of other microorganisms. Some antibiotics are synthesized or modified in the laboratory; however, their origins are always in living cells.

9.1 Assay of Antibiotics

Soil serves as the major repository of microorganisms some of which produces antibiotics. Four groups of soil microorganisms viz; Streptomyces, Bacillus, Penicillium and Cephalosporium represent three microbial types, namely, actinomycetes, true bacteria and molds respectively.

Following procedure may be applied to isolate antibiotic-producing microorganisms from soil:

Immunological Causes of Male Infertility

Immune mediated male infertility is an auto-immune disease.Spermatozoa are first produced at puberty and isolated from the systemic circulation by a structural barrier maintained by Sertoli cell tight junctions within the testis and the uninterrupted epithelial cell lining of the excurrent ductal system. Sperm production occurs well after the development of immunological tolerance to self antigens; therefore sperm specific components are viewed as foreign by the immune system. Whether spermatogonia, the primordial cell in spermatozoa differentiation, is also antigenically foreign is not established. What is known , is that immunization with sperm elicits a highly reproducible immune response that can impair fertility.

Antisperm antibodies are found in about 10-15% of infertile men. Immunity only to those antigens present on the surface of viable, motile sperm will impair fertility. Antibodies to internal sperm antigens, due to an immune response to sperm degraded in the genital tract or to cross reacting microbial antibodies present in the sera of many fertile individuals , are of questionable relevance to infertility. A number of spermatozoa antigens have been identified .

Fermentation

The term fermentation is loosely used to denote any process in which microorganisms are involved, regards of whether or not the organism is growing in the presence of molecular oxygen. Thus, it can be defined as 'an ATP generating metabolic process in which organic compounds serve both as electron donor (becoming oxidized) and electron acceptor(becoming reduced). Energetically, fermentations are poor energy yielding process and products have lower energy content then the substrates. Since there is no net oxidation in fermentation, the number of molecules of C, H and O remain the same in products as in the substrate.