eChapters

When you are assigned with your thesis problem or some problem is puzzling you, the first step is to search the related literature ; here the term literature is used not as it is used in humanities but this means the literature related to the research work published by the earlier scientists on the topic. The searching of literature is a most important and first step in conducting research on a particular topic. This not only acquaint you with the work of other scientists who tried to solve the problem but also confirms the possibility that previous scientists might have solved that problem and yours will be a repeat attempt ; in that case you may tackle other aspects of the problem, if at all they are existing and important in present circumstances.

There is only one type of scientific research and that is to solve a real problem or to understand the universe and provide a solution –either with the experiments or by putting a hypothesis. However, we have classified the research in various types as per our comfort. But for the employed researchers, it may be a matter of concern if they are working on time bound research schemes as their services may be co-terminus with the project and they may face the problem of un-employment, after termination of the scheme. According to the scholars’ activities and type of research being carried out in the country, we can discuss the subject under following topics.

One prime fact in research is not to take the matter for granted and do not believe in status quo. This is most critical mindset for creating something new. Even change may be made in existing circumstances with minimum requirements. Here, I may cite my experience while starting research in 1976 for my doctorate program on immunity against schistosomiasis. The experimental model was albino mouse where schistosomes, by their nature, develop into blood vascular system of the host hence require a special technique called “Perfusion technique” for retrieval of mature and immature schistosomes. This ‘perfusion technique’ requires use of a “Peristaltic pump” and was followed in some western countries (2). However, No body in India followed this perfusion technique hence recovered schistosomes from mouse or from any other host species by teasing the blood vessels or/and cutting and soaking liver,spleen, kidney, lung pieces in normal saline.

If you have prepared yourself to make research as your career, it is most advantageous to read research papers/reviews/chapters/books published by the eminent scientists. It is not necessary to confine reading to your subject but a voracious reading will enable you to develop an idea about general problems related to science and our society; how scientists managed to solve them and with development of new knowledge, how you may tackle fresh or existing problems. A deep analysis may be done of those works which are so accurate that even after lapse of so many years, they still hold true. In our opinion, it is better to launch websites/blogs highlighting important works of eminent Parasitologists for the benefit of our research scholars; a beginning, in India in Parasitology, has been made in this direction with launch of a website on first time Rafi Ahmed Kidwai award winner (in Animal Sciences) late Dr SC Dutt (www.drscdutt.com). 

It is always advantageous if there is a senior scientist,a mentor or a Guru ( I have not used the term teacher as all may be teachers but may not be Guru as per Indian mythology) who can recognize your talents and may believe that you may achieve some unusual goals, if properly guided or encouraged. This is important for carrying an important task to its fruitful results. Here what we are referring a Guru is not in the sense as is presently used for searching a god father. There is a vast difference between the two. The perception about god-father is that he may not possess the required knowledge but is perfect in manipulating situations in his favor so that work is done by all means. In scientific world, this is mostly applied to the persons who have contact with higher ups helping in getting promotions or foreign trips. These god fathers are good only to provide some material gifts but are not best in their scientific achievements hence cannot replace a Guru or a good scientist.

A close look on three disciplines of Parasitology reveal that these are the branches where a scholar initially select them as per his basic qualifications. But while pursuing research in Parasitology, the line of demarcation becomes blurred and it is in fact combination of a group of scientists,from varied disciplines, who are able to solve any one problem; at that point, it is absurd to divide the subject as medical or veterinary or basic parasitology. In fact, these will be your interests and experiences which will decide your line of research and will determine your achievements.

Parasitology is the branch of biological sciences that deals with the study of parasites and all the related aspects. This leads to a basic question who is a parasite ? In fact, this term is frequently used in our society for those persons who are doing nothing and depending on others for their survival. The term Parasite means the organism (parasite) which depends on other organism (host) for its nutritional requirements. By definition, it excludes the organisms which are free living and do not depend on other organism for their food requirements. There are other details in fulfilling its nutritional requirements and important one is that in doing so, it causes some harm to the host other wise it will not be a parasite but a commensal.

Our nature is the greatest or only laboratory where a large number of events are occurring at every fraction of a second - there are so many events, creatures, miseries taking place in the nature. Rightly it is considered an unparallel and biggest laboratory, man has ever witnessed. However, understanding it’s any event requires deep devotion and understanding,then only success will come. Our Philosopher and Poet Kabirdas has understood these facts as have reflected in his various couplets “Jin khoja tin paiya, gahre pani paith” or “chalti chaki dekh kar diya kabira roye, do patan ke beech main sabit bacha na koi“. For an ordinary person earth is still flat and he may not know the reasons for change of seasons.

It will be unjust not to describe history of Parasitology (31) and a little bit about Parasitology (chapter 8) so that our students may know the earlier works done in this field and how research has advanced by diligent observations of the eminent scientists when they were not having such sophisticated facilities.

As helminth is visible with naked eyes, it was this parasite which man encountered first in its journey of life sciences or natural sciences,as was known in ancient times. Some of these were known even before the era of biblical Palestine and ancient Egyptian civilization. Hippocrates (460-357BC), ‘Father of Medicine’ knew about pinworms (Oxyuris equi) of horses. In his “History of Animalium”, Aristotle (384-322 BC)(32) has mentioned three kinds of helminthes-flatworms, cylindrical worms (Ascaris lumbricoides) and thinworms (Enterobius vermicularis). The treatises of India had the information on large roundworms of intestine (Ascaris), pinworms, leeches, bed bugs and mosquitoes.

Whenever a person acquires desired qualifications, he needs a forum to express his talents and a place where he can fulfill his dreams and make important attainments. This may be achieved by two ways. One is, he becomes an entrepreneur and plans his own organization. As we are discussing biological research, there are good opportunities to start a Pharmaceutical firm to produce a drug, or a biotechnology firm to produce some biochemical molecule, enzymes, bio-fertilizers, vaccines against important human/animal/poultry diseases or a firm to supply diagnostic kits or immunological products etc. These are not hypothetical suggestions, difficult to achieve by a middle class person, but part of reality achieved by middle class family in India with laurels in their field of work. We may cite examples of firms like Dr Reddy Laboratory (Dr K.A Reddy), Biocon India (Ms Majumdar), Venky (poultry vaccines etc), Biorad (Bangalore based biochemical molecules supplier) who are working in India and leading their respective field. All this have been possible with tremendous change that has occurred in Indian scenario where there are many venture capitalists (or PE firms) who are willing to invest any amount of money provided they like your new idea of establishing a new enterprise. A somewhat more detailed information, in this regards, is provided elsewhere (see Research for money-chapter 13). Here I have not mentioned to pursue a Dairy or Poultry farm as these are not parts of research activities but related to animal production and any other person may also enter in such commercial activities.

At times, we hear our young generation questioning the need for obtaining degrees for conducting any research work. To some extend they are right. If we look back, there are many examples where persons without any former degree attained what is impossible by many degree holders. Tulsidas has written ‘Ramacharitmanas’, incorporating so many principles of Vedas and stories from Puranas without obtaining any degree. Srinivasa Ramanujan (21) narrated mathematics theorems without any degree in mathematics. His achievements may be appreciated by the fact that we celebrate his birthday 22nd December as National Mathematical day.

The very basics in one’s life is whether he has judged his interests or decided goals of his life or has taken life casually. The first is to know what you wish to achieve in your life or in the words of our former president and Bharat Ratna APJ Abdul Kalam (14) what are your dreams ? If you have dreams than only you can achieve them hence it’s essential to know what is your passion ?, passion is the key to success. In pursuing your passion, all things become secondary ; in fact, desired changes occur within you, unnoticed and automatic to achieve your goals. It may not be out of contest to mention that basically I belonged to a business family and being eldest son in the family, my father asked me to join family business, after doing my intermediate, with the liberty to join part time BA classes while carrying business activities. But business was not my passion hence I struggled to join to the academics and carried all my Veterinary education with distinctions - because of my interests. I am not sure what would have happened to my life have I joined least interested business line- perhaps a failed business man.

Research is the basics which have changed human life and surrounding environment what existed on earth million years ago into present form. As soon as man realized himself as a separate entity from other animals, he started thinking about himself, creatures co-inhabiting with him and the universe. In that sense, biological science is the oldest subject which fascinated man, though it was called natural sciences in ancient times. Perhaps, the first curiosity was about himself, his birth, death and alike matters. As soon as he realized mortality of life or faced death of his dear ones, he attempted to become immortal or ‘amar’. This is reflected by worshiping of ‘Asur ’(Hirnakashyap, Bhasmasur, Ravan etc) to ‘Brahma’ or ‘Shiva’ for receiving the blessings of becoming immortal . However, even God refrained to bless these personalities becoming ‘amar’ confirming the realization by all that life is strongly linked with death ; as such they were blessed with certain conditional ties which may lead their death. Eventually, they met with their death under specific circumstances (3). When God or His little part (Ansh) took birth on earth as incarnation, He also followed all the rules and regulations of cosmic laws. But He never tried to become immortal and left His body on the earth, after finishing His duties.

If you are inclined to make research as a career, the first question is why are you interested in research or making it as a career in your life -whether for money or to pass a cozy life in a government institute or to get fame or to solve some scientific problems - related to cosmos, Agriculture, animals, human health, search some medicinal plants or you have great passion for science and impressed with its power to find solutions for many universal problems ? Or it is un-employment, now rampant in every field, which is deriving you to join higher degrees and research. Many times, this unemployment or no vacancies in due time leads the young persons to continue their post graduation or joining doctorate program with the hope that some vacancy will arise in the ensuing period or a suitable job may fall after or prior getting a Ph.D. degree.

Many techniques are used in immunology to detect specific proteins (antigens) or antibodies in biological samples. These techniques are called immunotechniques. These methods are used for research and diagnostic purpose in many fields such as microbiology, biochemistry, endocrinology, hematology and in pathological laboratories. The techniques can be used to estimate proteins, drugs, hormones and toxic substances in a sample. Immunological techniques are highly specific, precise and reliable. These methods are simple and safe. Antigen and antibody reaction is highly specific in nature and this forms the base of the immunological methods. There are many immunotechniques which are used for the qualitative and quantitative estimation of antigens and antibodies in a sample. Some of commonly used techniques in immunology are:

One of the characteristics of the immune system is to recognize and discriminate self from the non-self antigens.  But, sometimes, there is a failure to discriminate between self and non-self antigens in the body and an autoimmune response occurs. Body starts producing antibodies against its own cells/antigens. These antibodies are called autoantibodies and this state is called autoimmunity.

The well-being of a person depends on his immune system. Any deficiency in any component of immune system may lead to defects in the normal functioning of his body and makes him more susceptible to diseases. The immunodeficiency may be either in specific components (B cells or T cells) or nonspecific component (phagocytes and complement) of the immune system.

A tumor is said to be an abnormal growth of cells to form a lump or mass of transformed cells. This growth occurs due to loss of regulatory control of cell division. The majority of cells and organs have a balance between cell renewal and cell death. A cell has a particular life span;when it dies a new cell is generated. In normal condition, this system is regulated through cell cycle and cell division but at the time of any abnormality this growth mechanism is totally disturbed and failed. These cells give rise to clone of the cells and eventually produce tumor.

Transplantation refers to the implantation of a tissue or organ from one site  to another site in an individual or from one individual to another.It involves implanting of organs or grafting of tissues to repair defects and to stimulate regeneration. Auto-transplantation or autoplasty of the skin, cartilage, bone, muscle, tendon, nerve and fatty tissue is used in plastic and cosmetic surgery. The replacement of a diseased organ by a transplant of a healthy tissue is called transplantation.

Immunity is defined as the resistance to infection. This is carried out by the process of identification and removal of non-self material. In contrast to this, our body has to maintain immune tolerance to our own cells and tissues and develop the ability to distinguish these self-antigensfrom non-self (foreign) antigens. Tolerance means unresponsiveness of the immune system to an antigen. Immune tolerance is the process by which the immune system does not destroy an antigen. Tolerance can be either natural or induced.