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Background
Milk is known as a whole food for human consumption. It is the first food of the newly born human mammals. Milk contains almost all the nutrients required for the patients, the elderly, pregnant mother and new born baby. There is no doubt that milk plays a significant role in human development. We can prepare many physical forms of milk like cheese, yogurt, ghee, and milk powder. The calcium content is higher in buffalo than in cows. Buffaloes are the second largest source of milk and produce 15 % milk. Higher biological value proteins, fat, soluble vitamins, fatty acids, amino acids, water-soluble vitamins, and calcium are all present in milk. The main antioxidant components of milk and milk products are sulfur, phosphate, zinc, and amino acids like cysteine (necessary to produce collagen).
Milk somatic cells are normal cells found in milk in small amounts (epithelial cells), some of which are leukocytes (white blood cells). When mastitis is infected or injured, leukocyte production rises. Milk with a low SC level produces superior milk products that last longer. Better milk management, better cleanliness, and correct nutrition all contribute to a decrease in milk SC, which indicates poor milk production hygiene and a cause of mastitis. Milk somatic cells are made up of both immunological and milk-producing cells. In terms of defense, somatic cells are the second line. During the routine process of milking, these cells are secreted in milk. Somatic cell count is a good tool for evaluating farm cleanliness in bulk milk and is utilized worldwide as an indication of udder health in individual milk. The most prevalent condition affecting dairy buffalo production and their health and well-being is mastitis. The somatic cell count consists of immune cells (75%), such as neutrophils, macrophages, lymphocytes, erythrocytes, and epithelial cells (25%). Investigators have made different attempts to exploit milk somatic cells as a potential non-invasive sample to diagnose early pregnancy udder health in bovines.

Background
Blood collection from cows and other animals is a routine procedure often performed for diagnostic, research, or health-monitoring purposes. Diagnostic testing, such as hematology, biochemistry, hormone assay, infectious disease testing, and animal pregnancy diagnosis, can be done using blood. Blood samples are required for genetic testing (DNA fingerprinting or parentage determination), pharmacokinetic studies, and immunological studies. Another use of blood collection is monitoring animal health.
It is essential to consider that proper techniques of blood sample collection should be followed. Avoid introducing infection to the animal during blood collection. Remember the animal welfare to minimize distress and discomfort to the animal during blood collection.
Materials Required
• Sterile syringe or vacutainer (usually 16-18 G needle)
• Blood collection tubes (BD Science K2 EDTA Vacutainer(R) PLUS 6 mL) for whole blood, serum tubes for serum collection, or other specific tubes depending on the test (Figure 1).
• Antiseptic solution (e.g., alcohol or iodine)
• Restraint (head gate or halter)
• Gloves

Dairy animals are the backbone of animal husbandry. India is the largest milk producer and ranks first in the world with a share of 25% of global milk production. Haemoprotozoan diseases pose significant limitations to the health and production of cattle. It leads to significant losses in the livestock industry worldwide. However, most protozoan parasites in the blood are known tocause anaemia by inducing erythro-phagocytosis. In terms of  mortality, reduced milk production and reduced immunity power, hemoprotozoan parasites pose a major threat to the livestock population. Theileria, Babesia, Anaplasma and Trypanosoma are the most important hemoprotozoan diseases of veterinary importance. These diseases affect different livestock species, caused by different species of Trypanosoma, Theileria, Babesia and Anaplasma. Tick-borne hemoprotozoan infections have a significant negative impact on the health and productivity of cattle and result in major losses to the global livestock industry. In tropical and subtropical regions, tick-borne hemoprotozoan infections have a significant impact on animal production. In India, these hemoprotozoan parasites have long posed a serious threat to the survival of exotic and crossbred cattle (Ananda et al., 2009). Haemoprotozoan diseases have deleterious impact on health and production of animals causing death in acute cases, production losses in chronically affected animals which decrease economic share of livestock sector (Bharti et al., 2022)

There are number of internal and external factors that affect the production performance of animals, among which parasites and parasitism are major of concern constraints to animal’s productivity especially in tropical countries. Feed intake and nutrient requirements have been recognized for long time as an important parameter for determining the efficiency of animal production. Most of the parasitic infection in domestic animals is caused by gastrointestinal nematode, which may result in depression in appetite, impairment in GI functions, alteration in protein, energy and mineral metabolism and changes in water balance. Nutrition can affect the ability of the host to cope with the consequences of parasitism and to contain and eventually to overcome parasitism. Since it is proposed that the host gives priority to the reversal of the pathophysiological consequences of parasitism over other body functions, it is to be expected that improved nutrition will always lead to improved resilience. On the other hand, it is proposed that the function of growth, pregnancy and lactation are prioritized over the expression of immunity. Improved nutrition may affect the degree of expression of immunity during these phases and can thus influence the resilience and resistance of host to parasite infection.

Livestock has been essential for Indian agriculture since prehistoric times. Livestock is crucial for tripling farmers’ revenue, for bovines—particularly cattle and buffaloes—play a crucial role in the economy. India has the
largest number of livestock owners, with a wide variety of livestock and poultry breeds that are essential to the socioeconomic advancement of rural communities. The production of consumables and maintaining the country’s food security are inextricably related to the livestock and agricultural sectors. Numerous illnesses have a detrimental effect on the health and productivity of livestock and are highly significant economically. Livestock illnesses have negative effects on producers, costing them money in upkeep, resources, and production.
Protozoal infections are a major contributor to the global burden of infectious diseases and have a profound effect on health, society, and the economy. Nearly one-sixth of the world’s population is at risk of dying from serious diseases like malaria (Plasmodium spp.), African sleeping sickness (Trypanosoma brucei), Chagas’ disease (Trypanosoma cruzi) and various forms of cutaneous and visceral leishmaniasis (Leishmania spp.). Although the burden of protozoal diseases primarily affects tropical and subtropical regions, environmental changes and ecological modifications brought about by both natural and manmade factors have had and are likely to continue to have a significant impact on the emergence and spread of these infections in high-income nations.

Haemoprotozoan parasites are responsible for causing severe infections in humans as well as animals across the world. The important hemoprotozoan diseases of livestock are trypanosomosis, theileriosis, babesiosis, and
anaplasmosis, which are caused by several species of Trypanosoma, Theileria, Babesia, and Anaplasma, respectively, in several species of livestock. These diseases caused huge impact on health and productivity of livestock as well as human beings and causes great economic losses to the livestock production n terms of morbidity and mortality worldwide. In India, the annual economic loss has been esteemed in the livestock due to theileriosis, babesiosis and trypanosomosis of Rs 8426 crore, Rs 4000 crore and Rs 4474 core, respectively. The major clinical signs of the haemoprotozoan disease varies from fever, anorexia, anaemia, abortion, and even death in the acute form of infections. The symptomatological treatment is a quite common practice in the field condition to treat the animals suspected with haemoprotozon infection leading to the development of resistance in animals to currently available drugs against haemoprotozoans. The accurate and prompt diagnosis of haemoprotozoan infection is a key to control these diseases. Routine diagnosis of haemoprotozoan infection has been rely on clinical findings and microscopical demonstration of the infective parasitic stages in the blood or tissue fluid smear. Conventional diagnostic techniques such as Microscopical examination techniques and Immunological assay provide the definite clues about parasitic infections in general, but these tests have some limitations.

The reclassification of the order Rickettsiales was predicated upon comprehensive genetic analyses of 16S rRNA, groESL, and surface protein genes. Consequently, organisms within this order were systematically assigned to one of two families: Anaplasmataceae and Rickettsiaceae. Within the Anaplasmataceae family, phylogenetic analyses consistently delineated four genetically distinct clades: (1) Anaplasma, (2) Ehrlichia, (3) Wolbachia, and (4) Neorickettsia. Despite the obligate intracellular nature of rickettsial organisms in both families, those classified under Rickettsiaceae proliferate freely within
the host cell cytoplasm, whereas members of the Anaplasmataceae family are confined exclusively to membrane-bound vacuoles within the cytoplasm of host cells.
The first documented cases of anaplasmosis trace back to the late 19th century in South Africa. However, it wasn’t until 1910 that the causative agent of the disease was conclusively identified morphologically as “marginal points” within red blood cells by Max Theiler, later named it Anaplasma marginale. A year later, he identified another species, A. centrale, which caused a milder form of the disease in cattle. During the 1930s, Sanborn, Stiles, and Moc demonstrated the mechanical transmission of A. marginale by horse flies and stable flies. In the late 1980s, the life cycle of A. marginale was fully elucidated in ticks belonging to the genus Dermacentor.

Bovine babesiosis is a significant febrile disease, primarily affecting cattle and buffalo, transmitted by ticks. The disease is marked by the rapid proliferation of parasites within the bloodstream, leading to the extensive destruction of red blood cells. This results in a cascade of clinical symptoms, including anemia, jaundice, hemoglobinuria, and splenomegaly, with the potential for severe cases to culminate in death. The acute form of bovine babesiosis is particularly severe, often characterized by these pronounced symptoms, which can lead to rapid deterioration in the affected animals. Babesiosis is not merely an acute illness but can also persist as a chronic infection. Chronic bovine babesiosis is typified by ongoing anemia and varying degrees of weight loss, reflecting the enduring impact of the parasite within the host. Economically, this disease poses a formidable challenge, especially in regions where cattle are most susceptible. Since the first description of Babesia bovis by Victor Babes in Romania in 1888, numerous distinct species have been identified, each contributing to the global burden of this disease (Hunfeld et al., 2008). The impact of bovine babesiosis on livestock improvement efforts in endemic regions is profound. The introduction of susceptible, naive animals into these areas often leads to significant losses, as these animals are highly vulnerable to the disease.

Theileriosis is a tick-borne protozoan disease that affects a wide range of domestic and wild animals, particularly cattle. While multiple species of Theileria exist, Theileria parva and Theileria annulata are the most important pathogens in terms of their impact on domestic cattle. This disease is transmitted by ixodid ticks and has a significant impact on cattle populations in tropical and subtropical regions. Theileriosis poses a substantial threat to livestock productivity, affecting economic stability in many countries, especially those that rely heavily on agriculture and cattle farming.
The disease has two primary forms: East Coast fever (ECF), caused by T. parva, which predominantly affects cattle in eastern, central, and southern Africa; and tropical theileriosis, caused by T. annulata, which affects cattle across northern Africa, the Middle East, and parts of Asia. Both diseases are highly pathogenic and, if left untreated, can lead to high morbidity and mortality rates, significantly impacting livestock production.

Kingdom: Protozoa
Phylum: Euglenozoa
Class: Kinetoplasta
Order: Trypanosomatida
Family: Trypanosomatidae
Genus: Trypanosoma
Members of the genus Trypanosoma are responsible for trypanosomosis in animals and human beings and are found in the blood vascular system and tissues all over the world. All the species of trypanosomes are transmitted biologically or mechanically through an arthropod vector except T. equiperdum, which is transmitted through coitus in equines.

Protozoa are considered as unicellular eukaryotic organisms and evolved after bacteria and thousands years early to multi-cellular organisms as well as animals. This unicellular eukaryotic organism contains all the organelles or structures like multi-cellular organisms but in single cells. Similar to multicellular, the genetic information of protozoa is also stored in chromosomes. The protozoa differ from bacteria in different manners. The differentiating features of bacteria and protozoa are given in Table 1.
1. General Structures of Protozoa
Protozoa, like other eukaryotic cells, contain a nucleus, endoplasmic reticulum, mitochondria, Golgi apparatus, and lysosomes. Beyond these common organelles, protozoa feature a range of unique structures with specific functions that enable them to operate independently. Protozoan cells can be categorized into two primary components: the cytoplasm and the nucleus.

Receiving student award from Dr IP Singh Dean Veterinary Sciences
Receiving student award from Mrs (Dr) Shinade Acting Vice-Chancellor
Dr Rajendra Prasad award of ICAR-2002 (Union Agriculture Minister Shri Ajit Singh is giving award to Prof RS Chauhan in the presence of Shri Hukum Dev Narayan Yadav Minister of state and Secretary agriculture)
Receiving “Gopal Gaurav award” from Shankaracharya Shri Raghvendra Saraswati ji maharaj of Shri Ramchandrapuram muth, Shimoga Karnataka ^^xksiky xkSjo** lEeku izkIr djrs gq,
Dr Jeff G Vos Congratulating Prof RS Chauhan on becoming advisor WHO at NIPHE Bilthoven, The Netherlands.
 Dr RS Chauhan is with the Union Agriculture minister Shri Sompal Shashtri ji in a function of Prakrati Bharti at Kota (Rajasthan)
Dr RS Chauhan is with the Union Science & Technology minister Shri Bachi Singh Rawat ji in a function of SIIP at CIMAP Lal kuan (Uttarakhand)

1. Ambwani S, Ambwani Tanuj Kumar and Chauhan RS (2014) Counteracting effects of cow urine on allethrin induced immunotoxicity and oxidative stress in chicken lymphocytes culture system. Journal of Immunology and Immunopathology.16: 58-63.
2. Ambwani S, Ambwani TK and Chauhan RS (2018) Ameliorating effects of Badri cow urine on cypermethrin induced immunotoxicity and oxidative stress in chicken lymphocyte culture system. Biosciences Biotechnology Research Asia.15 (3): 1-12.
3. Banga RK, Kumar P, Singhal LK, Sharma A and Chauhan RS. 2005. Red hill cattle is characters and body measurements. The Indian Cow, 3: 10-14.
4. Banga RK, Singhal LK and Chauhan RS. 2005. Cow urine and immunomodulation: An update on cowpathy. International Journal of Cow Science, 1(2): 26-29.
5. Chauhan Divya and Chauhan R.S, 2021. Badri Cattle - A Boon to Uttarakhand. In: Cowpathy and Human Health ( Eds : Sethi RS, Kaur Gurvinder, Malik Yashpal, Chauhan RS ). SIIP, Ludhiana. P_17
6. Chauhan Divya and Chauhan R.S. (2022). Cow Science Experts in India - 1. Shree Krishna A well known Cow Lover. International Journal of Cow Science. 6(1):2
7. Chauhan R S and Tulsa Devi. 2020. Immunity, Nutrients and Immunomodulation. International Journal of Emerging Technologies and Innovative Research, 7 (5):1030-1038.

I have known Dr R.S. Chauhan al through his professional carrier. I came in his contact when he was a student, a research worker, a professional colleague. He is very hard working, sincere and dedicated scientist and teacher. I observed his remarkable skills, unwavering dedication and strong commitment for upliftment of research and the profession. He is known as an excellent teacher. He devised simple understandable means for complex topics.
His work on Panchgavya has won lots of appreciation at all levels. In fact he is a person who proved scientifically the application of components of Panchgavya on control of some untreatable disease like cancer.
I have absolutely no hesitation in saying that he is the “Father of Cowpathy”. He proved the efficacy and application of products of cow origin with scientific validation.
To be true, even today whenever I have some query, I invariably consult him because I am of the opinion that he has a very strong basic background. May God always shower his blessings on him.

Dr. Chauhan, a dedicated veterinary scientist, has built a remarkable reputation for his tireless efforts in animal welfare. His journey into veterinary medicine was not just a career choice but a calling, deeply rooted in his compassion for animals and a firm belief in their right to a healthy, dignified life. Over the years, he has not only saved countless animals but also played a crucial role in shaping community attitudes towards animal welfare. His engagements span various domains, including free medical camps, rescue missions, awareness campaigns, collaboration with NGOs, and advocacy for animal rights.
Free Veterinary Camps in Rural and Urban Areas One of Dr. Chauhan’s most impactful contributions has been his organization of free veterinary camps. These camps, held in both rural and urban areas, provide essential medical aid to animals that might otherwise go untreated. Many pet owners and farmers struggle with the financial burden of veterinary care, and Dr. Chauhan’s initiative ensures that their animals receive timely vaccinations, deworming, and treatment for common illnesses.
In rural areas, where livestock plays a critical role in the livelihoods of farmers, these camps have been particularly beneficial. By treating cattle, goats, and poultry, Dr. Chauhan has helped improve the overall health of farm animals, leading to better productivity and economic stability for local communities. In urban areas, his focus extends to street animals, offering free treatments for injured or ailing stray dogs and cats.

As he looks to the future, Dr. Chauhan envisions several key goals to further the field of veterinary medicine: Expanding the scope of Veterinary Science :
Future goals of Dr. Chauhan
1. Advancing Research in Veterinary Pathology: Building upon his extensive research background, which includes authoring 120 books, contributing 101 chapters, and publishing 239 research papers, Dr. Chauhan aims to delve deeper into emerging diseases affecting livestock and companion animals. He plans to focus on molecular pathology to better understand disease mechanisms at the genetic level, facilitating the development of targeted treatments and preventive measures.
2. Promoting ‘Cowpathy’ and Indigenous Practices: Known as the “Cowpathy Man,” Dr. Chauhan has been a proponent of integrating traditional knowledge with modern veterinary practices. He intends to conduct rigorous scientific studies to validate and promote the use of indigenous products in animal healthcare, aiming to offer sustainable and eco-friendly alternatives to conventional treatments.
3. Enhancing Veterinary Education: With a passion for veterinary education, Dr. Chauhan plans to develop comprehensive curricula that incorporate the latest advancements in veterinary science. He seeks to mentor the next generation of veterinarians, emphasizing the importance of ethical practices, continuous learning, and adaptability in the face of evolving challenges in animal health.

Dr. Chauhan’s philosophy as a Veterinary Pathologist reflects a profound understanding of the interconnectedness of life and an unwavering commitment to the well-being of animals. His approach is rooted in compassion, scientific rigor, and a holistic appreciation of the delicate balance that sustains ecosystems. Over the years, he has become a beacon of hope for gaushalas and cow enthusiasts, demonstrating that veterinary medicine is not just a profession but a calling that demands empathy, dedication, and an unyielding respect for life.
At the core of Dr. Chauhan’s philosophy is the belief that every living being, regardless of its size or species, deserves dignity and care. To him, animals are not mere creatures but sentient beings with emotions, instincts, and intrinsic value. This perspective shapes his practice, as he treats each animal with the same care and attention he would give to a human patient. Whether it’s a stray dog in need of emergency care, a farm animal requiring preventive medicine, or an endangered species in a conservation program, Dr. Chauhan approaches his work with equal seriousness and respect.
Dr. Chauhan emphasizes the importance of understanding animals within the context of their natural behaviors and environments. He often says, “To heal an animal, you must first listen to what it cannot say.” This insight has driven him to study not only veterinary medicine but also animal behavior, ecology, and the complex relationships between humans and animals. He believes that understanding these dynamics is essential for providing comprehensive care. For example, he advocates for preventive care practices that align with an animal’s natural rhythms, such as vaccination schedules that account for seasonal changes and diets that mimic an animal’s natural food sources.

Dr. Chauhan’s illustrious career as a Veterinary Pathologist is marked by resilience, unwavering compassion, and a commitment to animal welfare. Despite facing numerous challenges, his journey serves as an inspiration to many, not only for his professional achievements but also for his ability to turn obstacles into stepping stones for greater success. Over decades of practice, Dr. Chauhan became a symbol of hope for animals and their caregivers, a testament to what dedication and empathy can accomplish.
Breakthroughs and Milestones
Dr. Chauhan’s career is filled with milestones that showcase his determination to push the boundaries of veterinary medicine. Early in his career, he made headlines by successfully treating a prized racehorse suffering from a rare neurological disorder. The case, which had been deemed incurable by others, required not only medical expertise but also the courage to think outside the box. Dr. Chauhan used a combination of experimental techniques and traditional therapies to restore the horse to full health. This triumph not only saved the horse’s career but also cemented Dr. Chauhan’s reputation as an innovative problem-solver.
Another significant breakthrough came when Dr. Chauhan devised a novel treatment protocol for canine distemper, a disease that ravages stray dog populations. By combining immunotherapy and supportive care, he achieved remarkable survival rates, helping communities across the region manage outbreaks more effectively. One of his most celebrated cases involved Tara, a critically injured elephant rescued from an illegal logging operation. Tara had sustained multiple fractures and severe infections, and her chances of survival were slim. Dr. Chauhan spearheaded an intensive six-month treatment regimen, which included groundbreaking orthopedic surgery and customized physiotherapy. Tara’s recovery became a symbol of hope for wildlife conservationists and sparked a nationwide discussion about the ethics of using animals in hazardous labor.

Dr. Chauhan, a renowned figure in the field of veterinary science, has left an indelible mark on the discipline through his groundbreaking research, innovative teaching, and unwavering dedication to the advancement of animal health and welfare. Over the years, his exceptional contributions have earned him a multitude of prestigious awards, medals, and honors, making him a luminary in his field.
His illustrious career began to gain recognition with the Best Young Scientist Award in 1992by Indian Association of Veterinary Pathologists, an acknowledgment of his potential and early contributions to veterinary science in general and Veterinary Pathology in particular. This award marked the start of a journey filled with accolades that would underscore his relentless pursuit of excellence. Four years later, in 1996, he was honored with the IAAVR Award, further solidifying his position as a promising researcher and academic.
By 1999, Dr. Chauhan’s expertise and dedication had earned him the National Fellow Professor chair of ICAR, a prestigious recognition that highlighted his growing influence in veterinary research. Around the same time, he was conferred the title of Fellow NAVS (2000) and Fellow SIIP (2001), reflecting his significant contributions to national and international veterinary organizations. These fellowships were a testament to his role in advancing scientific understanding and his collaborative efforts within the veterinary community.
Dr. Chauhan’s profound impact on the field was also recognized with several specialized awards, including the K.S. Nair Memorial Award in 1999, the Vigyan Bharti Award in 2000, and the Dr. C.M. Singh Trust Award in 2002. These honors celebrated his ability to merge academic rigor with practical applications, benefiting both the academic community and the agricultural
sector. Similarly, the Dr. Rajendra Prasad Award (2002) and the Shri Ramlal Agrawal National Award (2000) underscored his contributions to veterinary science in India.

Dr. Chauhan is an exceptional individual who had achieved a significant milestone in his academic and professional journey. Completing a Bachelor of Science (Honours) with an impressive 80.3%, Dr. Chauhan’s accomplishment is a reflection of his hard work, dedication, and unyielding passion for the field of veterinary science. This remarkable achievement not only underscores his
academic brilliance but also cements his position as an inspiring figure in the veterinary community.
From an early age, Dr. Chauhan demonstrated an innate love for animals and a deep sense of responsibility toward their well-being. These qualities formed the foundation of his journey in veterinary science. His BVSc&AH (Honours) degree, completed with such distinction, is not just a testament to his intellectual capabilities but also to his tireless commitment to improving animal health and welfare. Pursuing a degree in Veterinary science is no small feat. It demands a unique combination of scientific expertise, practical skills, and emotional resilience. For Dr. Chauhan, the rigorous academic journey involved mastering a wide array of subjects, including animal Anatomy, Physiology, Pathology, Pharmacology, and Microbiology. Each subject posed its own set of challenges, yet he tackled them with unwavering determination and an insatiable thirst for knowledge. His exemplary performance, as evidenced by his impressive score of 80.3%, highlights his ability to excel even under pressure. What sets Dr. Chauhan apart is not just his academic excellence but also his holistic approach to learning. Beyond textbooks and lectures, he actively sought opportunities to gain hands-on experience, volunteering at animal shelters and participating in community outreach programs. These experiences not only enriched his understanding of animal care but also shaped his perspective as a compassionate and empathetic veterinary professional.