eChapters

Spiralling whitefly : Aleurodicus dispersus : Aleyrodidae

A. dispersus originated from the tropical countries in Central and South America. More recently, it has been reported from Asia and Africa. In India, Andhra Pradesh, Karnataka, Kerala, Maharashtra and Tamil Nadu faces its problem. A. dispersus is a highly polyphagous species. Its wide host range includes many vegetable, ornamental and fruit crops, as well as numerous trees and shrubs, including Capsicum, Citrus, Cocos nucifera (coconut), Euphorbia pulcherrima (poinsettia), Glycine max (soybean), Hibiscus, Lycopersicon esculentum (tomato), Mangifera indica (mango), Musa (banana), Persea americana (avocado), Prunus spp., Psidium guajava (guava)and  Solanum melongena (brinjal) etc.

Key Points

• In dairy cows, anestrus is a prevalent reproductive condition.

• In dairy farm animals, infertility, pyometra, and repeat breeder cows are the main reproductive syndromes.

Introduction

Farm animals have been raised and chosen specifically for the production of meat and dairy over the years. The livestock business suffers significant f inancial losses as a result of farm animal infertility. If the open span is extended by one estrous cycle as a result of infertility, this explains the 20–25 day loss of output in addition to the expense of treatment, missed work, etc. Farm animals can become infertile for a variety of reasons, including nutritional deficiencies, physiological disorders, and viral infections. These factors can act alone or in concert.

Introduction

Climate change and pollution are the two major concerns that retard growth in animal sector particularly in a developing country like India. Pollution is directly contributed by human beings, while the former is also an after effect of continuous abuse of nature and natural resources. Hence these two areas are to be looked into seriously for country’s growth in general and animal production in particular.

Stress Effect

Stress is nothing but inability of an animal to cope with its environment, a phenomenon that is often ends up with a failure to achieve the full genetic potential. Stress may be from any origin and can even be a disease itself. There exists a variety of endocrine regulatory points of stress which limits the efficiency of production and reproduction. A simple transport produces an immediate constant increase in arginine, vasopressin and corticotrophinreleasing hormone secretion in animals. However, adenocorticotrophic hormone reaches a maximum in the first hour, while cortisol is highest during the second hour. Contrastingly, the hypothalamo–pituitary–adrenal response is delayed occurring only after glucose decreases below a threshold in an insulin treated animal. Negative feedback effects appear to operate mainly at the pituitary level during transport but at the hypothalamus during hypoglycaemia.

Principle
Some viruses like Orthomyxo, Paramyxoviruses etc. have the property to clump the erythrocytes of certain species of birds or animals. This is not an antigen-antibody reaction but a network of erythrocytes formed by the bridging virions. These virions on their envelop have peplomers that binds with the receptors on the erythrocytes. If large quantities of virus are used for HA and allowed to act on red blood cells
for a long period of time, the agglutinated red cells shed the virus particles which then float freely in the suspending fluid and neither attach to the same red cells nor agglutinate them. This process of dissociation of virus from the red cells is known as elution, and is due to the destruction of receptors on red cells by the virion associated receptor destroying enzyme - neuraminidase. The red cells freed from the virus particles are known as stabilized red cells. The elution will occur most rapidly if the temperature is raised to 37°C as the enzyme neuraminidase will be more active at this temperature.

Practical Application of Haemagglutination
a) Spot test: For rapid detection of the presence of a haemagglutinating virus. This can be done by mixing two or three drops of suspected sample (infected chick embryo fluids or cell culture fluids) with equal quantity of 5% chicken red cells in normal saline. The appearance of the clumps of agglutinated red cells, indicate the presence of a haemagglutinating
virus in the sample.
b) Titration of the virus using the haemagglutinating property.

It is a benign tumor of endothelial cells. It is a common tumor of dogs. Solar induction is one of the cause for development of this tumor.

Haemangiosarcoma is a malignant tumour of vascular endothelial cells. It is more commonly seen in aged dogs.

Clinical examination of animals regardless of domestic or wild ones is of utmost significance during the assessment of health status in animals. Prior to collecting samples, it is important to determine the capabilities and submission criteria of the laboratory receiving the samples.

Hyperaemia and Congestion
Definition
Hyperaemia is increased volume of blood in affected tissue or part.
Hyperaemia (Active Hyperaemia)
Occurs in arterioles or arteries
Increased blood flow in capillaries
Congestion (Passive Hyperaemia)
Occurs due to impaired venous drainage
Stasis of blood in veins
classification of hyperaemia

Introduction
The genus Haemophilus contains small, nonmotile, nonsporing, oxidase positive, pleomorphic, gram negative bacilli that are parasitic on human beings or animals. Haemophilus means blood loving organisms.

Haemophilus species are small (less than 1 pm x 1 to 3 μm), Gram-negative rods, which often appear coccobacillary and may occasionally form short filaments. These motile organisms, which are facultative anaerobes with variable reactions in catalase and oxidase tests, do not grow on MacConkey agar. They are fastidious bacteria requiring one or both of the growth factors X (haemin) and V (nicotinamide adenine dinucleotide, NAD). Optimal growth occurs in an atmosphere of 5-10% CO2 on chocolate agar which supplies both X and V factors. Small, transparent, dewdrop-like colonies are formed by most Haemophilus species after incubation for 48 hours.

Colonies of H.somnus have a yellowish hue and some isolates are haemolytic on sheep blood agar. The main pathogens in the genus are H.somnus in cattle and sheep, H.parasuis in pigs and H.paragallinarum which is responsible for infectious coryza of chickens.

The haemophili are motile, facultative anaerobes, produce acid from glucose, reduce nitrates and are variable in the oxidase and catalase tests. They are nutritionally fastidious, will not grow on MacConkey agar and grow best on chocolate agar (supplying the X and V factors) under 5- 10 per cent CO2 at 37°C.

The growth of many of the Haemaphilus species is enhanced by 10 per cent CO2, As this is not inhibitory for any of them, CO2 should be used for routine isolation.The inoculated chocolate agar plates are incubated under 10 per cent CO2 at 35-37°C for 3-4 days, although some growth may be seen after 24 hours.

A) Hypothyroidism

Etiology

It is one of the common endocrine disorders of skin of dogs mainly due to deficiency of thyroid hormone followed by cutaneous and non cutaneous signs. It is caused by lymphocytic thyroiditis or idiopathic thyroid necrosis and atrophy. Deficiency of thyroid hormone hinders the metabolic processes throughout the body.

Introduction

Use of acid/base is common in laboratories for experiments like titration and proper safety in their handling laboratory is necessary. In this chapter we will briefly discuss about the safety measures necessary to be adopted while using acids/bases in laboratory for any experiment.

Handling of Strong Acid/Base

There are four major points that need to be taken care of while working with strong acids or bases that might be quite harmful if any accident happens.

1. Protect Yourself

Wear a lab-coat and acid-resistant gloves: Always use lab-coat/apron and make it sure that the sleeves properly cover your arms. More importantly, use gloves made of acid-resistant materials and also wear shoes to protect your feet while wearing long-pants/jeans. Female with long hair, must tie her hair properly (with rubber band) to avoid any disturbance caused by appearance in between.

Wear safety goggles: It is important to wear safety goggles to protect your eyes using larger and fitting goggles that cover front as well as sides of your eyes. Always use properly fitting and adjustable goggles because smaller/ loose goggles may not protect the areas around your eyes against accidental acid spills and it may fall down from your face, if it is too loose.

Locate emergency wash area: There must be an emergency washing area nearby the work place or laboratory containing lab spill management kits. All the workers in the laboratory must be aware of using the kits and emergency plans.

Neutralize acid: Don’t panic even if an acid or chemical spill happens. Stay calm and act as quickly as possible. In case an acid spills, neutralize it by using sodium bicarbonate so that they can be cleaned and disposed safely. If acid comes in contact of skin, wash it off under running tap water but do not use/ apply sodium bicarbonate on skin.

Once the animal is darted, immobilized animal should be under the expert care of an experienced wildlife veterinarian. The veterinarian needs to monitor the complete immobilization process and should know what to do if something goes wrong. Keep the darted animal in sight and allow the drug to act completely which may generally take 5- 25 minutes depending on the species, sex, drug used, and body condition of animal. Any noise or excitement may cause immobilized animal to wake up or take flight which may make animal search difficult.

Introduction

For proper and efficient experiment to be conducted, experimenters need so many chemicals to deal with. This includes different acids, base, salt, different kind of indicators and so on. Some of these chemicals are safe for health, but some of them are dangerous to health when come in close proximity to our body while some of the chemicals cause health issues on inhaling. These may cause improper functioning of some of our physiological activities. Acids and bases are very dangerous for our tissues when come in our contact. These may cause serious respiratory problem in addition to injuries on coming to our contact. Some chemicals are exploratory yet they may cause even death of the person working with them in laboratory. Therefore, mere use of the chemicals in laboratory is not only the objective of the experimenter. The person working in laboratory should also be concerned about the proper use and handing of the chemical while working with it. Thus, every researcher of an institution who has to work with chemicals in a laboratory should be made aware about the chemicals to be used in the laboratory and the consequences if any mishandling of a laboratory chemical occurs. Not only these, suitable precautionary measures should also be known to the researcher along with ensuring the availability of the required safety equipment/installations. Hence, a proper understanding of the ways of handling of chemicals in a laboratory is of prime importance for the personals like you who are working or have to work in laboratory.

Safe Handling of Chemicals in Laboratory

Safe use/handling of chemicals in laboratory include minimized exposure to chemicals, maintaining safety data sheets, proper labelling, storage, segregation, and transport of chemicals for personal hygiene.

Introduction

We come across a variety of chemicals every day in a laboratory. While some of them are relatively safer, others are extremely hazardous. Thus, it is very important to know the potential hazards that can be caused by the chemicals present in the laboratory and the safety measures needed to be followed to avoid the hazards. Additionally, we should also be aware of the steps necessary to be taken on such hazardous situations. According to IUPAC, a hazardous chemical mean the chemical which has the potential to cause damage in various ways. In a laboratory, there are chemicals that might be irritant, carcinogen, respiratory fibrogen, systemic poison, asphyxiant, flammable, etc. which have the potential to cause hazard to the workers or environment. Therefore, it is important to use appropriate procedure to reduce the risk and protect the health/safety of laboratory personnel, the public and the environment.

Types of Hazards Associated with Chemicals

Chemical hazards: The hazards caused by chemicals can be broadly classified into three categories.

1. Health hazard: This is also known as toxic effects of chemical. The risk associated with a chemical in laboratory depends on the extent of exposure and the inherent toxicity of the chemical. Many chemical reactions form the product that might be much more toxic than the reacting chemicals. For example, inadvertent mixing of formaldehyde (a common tissue fixative) and hydrogen chloride results in the generation of bis(chloromethyl) ether, a potent human carcinogen. For most of the chemicals, the route of exposure (through skin, eyes, gastrointestinal tract, or respiratory tract) is more important (Figure 17.1) that must be considered during risk assessment.

Hantaviruses have been responsible for a variety of human illnesses particularly haemorrhagic fever with renal syndrome (HFRS) and hantavirus pulmonary syndrome (HPS). There are currently 47 recognized sero/genotypes of hantaviruses with considerable geographic diversity. Each hantavirus type appears to be specific to a different rodent host. The different hantavirus types are associated with different types of disease both in terms of target organs and disease severity. HFRS is primarily a disease of the Old World while HPS is a disease of New World. Although the disease may have first been recognized as early as 1000 years ago in China, it affected >3000 United Nations Soldiers during Korean war (1950-1953). Now it is reported that hantavirus affect as many as 2 lakhs people each year in Asia and Europe. The agent of this virus remained unknown until 1978 when Lee and his associates isolated and named Hantaan virus, after a river in South Korea. Other viruses antigenically related to Hantaan virus were characterized and placed under the genus Hantavirus and family Bunyaviridae.

Over the past few decades, the sharp increase of incidences of hantavirus disease in humans throughout the world has been reported. There are 47 recognized hantaviruses with considerable geographic diversity. But the number of hantavirus virus types recognized continues to grow. Hantaviruses cause persistent infections in rodent reservoirs which directly impact transmission of these zoonotic agents between rodents and from rodents to humans. Environmental, 127 demographic and physiological factors likely interact to mediate responses to hantaviruses in natural rodent populations. Although not much work has been carried out, comparisons of human and rodent immune responses to hantaviruses may provide insights into why hantaviruses often are fatal for humans but cause only persistent infections in rodents.

Family: Ixodidae (Hard ticks)

General characters

•These ticks have hard chitinous shield called scutum on their dorsal surface, so they are called hard ticks. Scutum extends over the entire dorsal surface in male and only anterior one-third of dorsal surface in larvae, nymph and female.

 •Mouth parts are anterior and visible from dorsal aspect.

 •Eyes are one pair (if present) situated on the lateral margin of scutum.

•Spiracles are one pair and situated posterolaterally to the fourth coxae. 

 •Basis capituli or capitulum present at anterior, and have mouthparts and palps.

•The body of female may have one pair marginal grooves at lateral side of behind the scutum.

Introduction
Antimicrobial resistance (AMR) constitutes a profound global health crisis, jeopardizing the efficacy of antibiotics and posing a severe threat to the foundation of modern medical practice. The rise of multidrug-resistant (MDR) pathogens, which exhibit resistance to multiple classes of antibiotics, has significantly escalated the global burden of infectious diseases, complicating treatment protocols and increasing morbidity and mortality rates. According to the World Health Organization (WHO), AMR could lead to 10 million deaths annually by 2050 if current trends continue, accompanied by economic losses projected to reach trillions of dollars due to prolonged hospitalizations, increased healthcare costs, and reduced productivity. The traditional antibiotic development pipeline has stagnated, hampered by scientific complexities, high financial costs, and stringent regulatory frameworks, resulting in a critical shortage of novel antimicrobial agents. This necessitates the exploration of innovative strategies to combat AMR and preserve the utility of existing antibiotics. Among these strategies, Crispr-Cas (Clustered Regularly Interspaced Short Palindromic Repeats and Crispr-associated proteins) systems have emerged as a groundbreaking gene-editing platform with transformative potential for addressing AMR. Initially identified as a bacterial adaptive immune mechanism, Crispr-Cas has been repurposed to enable precise targeting of genetic elements, offering novel therapeutic, diagnostic, and environmental solutions. This chapter provides a comprehensive analysis of Crispr-Cas systems in the context of AMR, exploring their molecular mechanisms, diverse applications, current limitations, and future prospects across clinical, agricultural, and environmental domains. By leveraging the precision and programmability of Crispr-Cas, researchers aim to develop targeted interventions that mitigate the spread of resistance while minimizing collateral damage to beneficial microbial communities.

Tissue – Proliferating Potential of Cell Types
1. Labile Cells
• Continuously dividing cells
• e.g. epidermis, epithelial cells, bone marrow cells
2. Stable Cells - Quiescent cells
• Undergoes division occasionally
• Liver, kidney    pancreas, fibroblasts,
• Endothelial cells
3. Permanent Cells - Non–dividing cells: Neurons, muscle cells (cardiac, skeletal)

Heart failure in canine is being recognized with an increasing frequency throughout the world. It is the end stage pathological event progressively occurring in heart diseases. Heart failure is the condition in which heart is unable to meet the metabolic and nutritional demands of the tissues of the body due to insufficient cardiac output. Heart failure has also been observed with normal cardiac output in conditions like hyperthyroidism or anemia where tissue metabolic needs are increased.

Heart failure is not a disease but it is the end stage of heart diseases. Heart failure in horses is not very common as in dogs and cats. In horses it is characterized by the signs of forward failure, backward failure and fall in blood pressure.