eChapters

Key Points

• In diary animals, the health of the mammary gland (udder) is paramount.

• Any udder or teat disease state has a direct impact on milk output.

• Common udder/teet affections include: abscess, mammary gland hematomas, udder cleft dermatitis, udder edema, intramammary hemorrhage, teat damage, ulcerative mammillitis, udder dermatitis, and more.

Introduction

Mammary gland (udder) health is a crucial factor in animals used for production. The state of the udder determines the dairy industry's economics. The output of dairy animals is directly impacted by any udder ailment. Due to selection, breeding, genetic engineering, and animal management, animals under production pressure are more susceptible to a variety of diseases. More safety measures should be implemented in order to shield the udder from illness-causing factors. The many udder and teat affections and their therapeutic management are briefly discussed in this chapter.

Lactation is the process of milk secretion by the mammary gland. Lactation is the final phase of the complete reproductive cycle of mammals. Newborns of most species are dependent on maternal milk during the neonatal period. Lactation is therefore essential for survival of the species.

Cancer has gained considerable relevance in animals now-a-days owing to the increased awareness among people towards animal sufferings and pain. The diagnosis and management of neoplasm, therefore, represent the major challenges faced by a veterinary oncologist. Mammary gland tumours are among the most common canine neoplasm, but the vast majority of these tumours occur in intact female dogs. In canines, mammary gland tumours that occur in males ranges from 0-2.7% (average < 1%). Sex hormones plays a vital role for the occurrence of mammary gland tumours. The p53 gene over expression is an independent factor for indicator of worse prognosis in canine mammary gland tumours. The p53 may be useful prognostic markers for evaluating malignancy in canine mammary gland tumours. There is a strong positive correlation between expression of cyclooxygenase-2 (Cox-2) and grade of malignancy in the tumours. Increased Cox-2 expressions are linearly related in the canine mammary gland tumours and are also related to worse prognosis and shorter overall survival. Canine mammary gland malignant tumours have the highest values of Cox-2 expression. Matrix metalloproteinase (MMPs) are a family of zinc and calcium-dependent proteolytic enzymes that degrade macromolecules of the extracellular matrix. Members of this family such as MMP-2, -9 and -7 have been shown to be associated with tumour progression and invasion in human cancer tissues. Matrilysin (MMP-7) influence the early stage of tumourogenesis.

Nuclear Disasters

What is NUCLEAR ENERGY or NUCLEAR POWER?

It is the energy released in nuclear fission or nuclear fusion.
Motive power generated by nuclear reactor.
Where atomic energy of a substance get converted in to electrical energy.

• General considerations

 • Ethics for veterinary diagnostic laboratory personnel

 • Safety measures in the laboratory

 • Accidents

 • First aid

 • Acid burns

 • Alkali burns

 • Poisoning

 • Burns caused by heat

 • Injuries caused by broken glass

 • Contamination by infectious materials

 • Bodily damage by electric shock

 • Precautionary measures

Goat husbandry has been practiced for thousands of years, since the first domestication of goats. Goat rearing for long mainly consisted of “zero input” concept, which suits well under extensive system of management. Major goat management systems in vogue are nomadic (transhumant)/extensive, semi-intensive and intensive, which have evolved on age-old experience and convenience of resource poor farmers under diverse agro-climatic and agro-ecological conditions.

Nomadic/Transhumant/Migratory/Extensive System

Extensive grazing lands cover vast areas of temperate Asia, and are important both environmentally and as a source of livelihood for goat and sheep herders.This practice is strengthened by migratory, free range, pasture cum range grazing options. The goats are compelled to often graze over long distances in search of feed and water. Under nomdic (transhumant) system, both grasslands and browse are important vegetation type. Land tenure problems, or lack of clarity in grazing rights are some of the emerging challenges for the goat herders under nomadic system. Many a times, the conflict of interests between settled farmers and herders is increasing with rising population pressure and intensification of agriculture. Poor pasture condition is one of the major constraint for the goat herders under nomadic system. Some of the measures suggested to improve the situation include better grazing management, reseeding with high-yielding species, and herder training.

Goat and sheep are two species which have been traditionally managed extensively. Mixed flocks of sheep and goats are common, although separate flocks of either species can also be seen. Extensively managed animals have considerably more behavioural freedom compared to intensively managed animals that are always more confined. Goat has an advantage over other species of livestock in that they can live and produce on land unfavourable for other forms of agriculture. Goat has the ability to forage and survive in areas, where cattle and buffaloes would perform poorly. Goat can revert to wild conditions if given the opportunity whereas sheep donot.

Mango Fruit fly : Bactrocera dorsalis : Tephritidae

This is probably the most economically important pest of mango. It can attack many crops like mango, guava, peach, apricot, cherry, pear, ber, citrus, banana, papaya, avocado, passion fruit, coffee, melons, jack fruit and strawberry. There are also related species that may cross infect. The adults are light brown small flies with a single pair of membranous transparent wings. The other pair is modified into halteres. Eggs are thrust into the skin of the mangoes when they are semi ripe. A drop of resin comes out of the ovipunctures and can be seen as a black mark. While purchasing mango, this can be used as an indication of the presence of the fruit fly eggs.

Marburg virus (MV) disease was named after the West German town where the largest outbreaks of human infection was reported. It is also known as Vervet monkey or green monkey disease as monkey species introduced the disease in Europe. Marburg disease virus was reported in 1967 in Frankfurt-on-Main and Marburg, West Germany and Belgrade, Serbia in vaccine factory workers who came in contact with blood, organs or cell cultures of African green monkeys. Ebola and Marburg hemorrhagic fevers are severe zoonotic diseases seen not only in humans but also in non-human primates. Most species of Ebolavirus and the only known species of Marburg virus occur in Africa. Primates are infected sporadically from an unknown source probably bats. Humans seem to become infected directly from bats in caves, as well as when they, come in contact with tissues from infected apes and other species. Once the virus has entered the population, it can spread from person to person. Some epidemics affect hundreds of people. Although the mortality rate varies, 90% case fatalities have been reported in the most pathogenic viruses. No vaccine is available and the only treatment is supportive. Epizootics in gorillas and chimpanzees are equally serious, and appear to threaten the survival of these species in the wild. Other wild mammals including duikers also seem to be killed during outbreaks. One species of Ebolavirus, Reston Ebolavirus, occurs in the Philippines. Until 2008, this virus was known only as an infection of nonhuman primates. It does not appear to be a human pathogen; although some people in contact with this virus have seroconverted, they remained asymptomatic. In December 2008, Reston ebolavirus was discovered in pigs during an unusually severe outbreak of porcine reproductive and respiratory syndrome (PRRS) in the Philippines.

Etiology: Ebola and Marburg hemorrhagic fever are caused by members of the genera Ebolavirus and Marburgvirus, respectively. These viruses are the only members of the family Filoviridae. The genus Ebolavirus contains four recognized species: Zaire ebolavirus (formerly Zaire Ebola virus), Sudan ebolavirus (formerly Sudan Ebola virus), Ivory Coast ebolavirus (formerly Cote d'Ivoire Ebola virus) and Reston ebolavirus (formerly Reston Ebola virus). At least 13 strains of these viruses have been identified. A fifth species, tentatively named Bundibugyo ebolavirus, was isolated from a recent outbreak in Uganda. Marburg virus contains a single species, Lake Victoria Marburgvirus (formerly Marburg virus). Six strains of Lake Victoria Marburgvirus had been recognized as of 1990; at least nine genetically distinct strains were identified from a more recent outbreak in the Democratic Republic of the Congo. Marburg virus contains ssRNA as genome. The virion is highly pleomorphic and measures 80 nm in diameter and 700-1000 nm in length. Filoviruses can be destroyed by autoclaving. Treatment with sodium hypochlorite (1:100 dilution of household bleach) or standard hospital disinfectants can also be used. Ebolaviruses are reported to be susceptible to 2% sodium hypochlorite, 2% glutaraldehyde, 5% peracetic acid and 1% formalin. These viruses can also be inactivated by ultraviolet light, gamma irradiation, 0.3% betapropiolactone for 30 minutes at 37°C (98.6°F), or heating to 60°C (140°F) for 1 hour. Marburgvirus is susceptible to 1% sodium hypochlorite, 2% glutaraldehyde or formaldehyde, ultraviolet light and heat.

Marek’s disease is a herpesvirus-induced neoplastic disease of chickens characterized by infiltration of various nerve trunks and/or organs with pleomorphic lymphoid cells. Marek’s disease is world wide in distribution. Marek’s Disease has been named after Josef Marek who published the disease in 4 roosters in 1907.

Occurrence

In chickens only, however turkeys and quail have limited susceptibility. Lesions similar to MD have been seen in pheasants, ducks, pigeons, geese, canaries, budgerigars, swans and great horned owls. However, aetiology for lesions in these species has not been proved. It may appear at 3-4 weeks but usually after eight weeks, with major losses from 12-25 weeks. Morbidity and mortality varies depending on the virulence of MDV.

Bud caterpillars : Helicoverpa armigera : Noctuidae

Eggs are laid singly on young buds.  Larvae feed on developing flowers by damaging florets. They can also eat the leaves.

Leaf webber : Phycita sp. : Phycitidae

Larvae of Phycita sp. feed on heads of buds and flowers.

Introduction
The emergence and spread of antimicrobial resistance has changed the landscape of infectious disease treatment, creating an urgent need for new diagnostic and therapeutic approaches4’7. Traditional phenotypic antimicrobial susceptibility testing, while remaining the gold standard, requires 24-72 hours for results and provides limited insights into resistance mechanisms. Limited turnaround time is often a constraint that requires empirical use of antibiotics, potentially driving the propagation of other resistance emergence as well as suboptimal patient outcomes. Thanks to mass spectrometry, clinical microbiology has greatly benefitted through the rapid detection of pathogens. However, the application is far broader than species identification. The integration of multiple omics fields like proteomics, metabolomics, genomics, and transcriptomics through mass spectrometry provides unprecedented means to unravel, predict, and combat antimicrobial resistance (AMR) on a molecular level. The use of a multiomics approach is demonstrated to show that AMR is a complex phenotype that arises from the intersection between genetic mutations, variations in gene expression, metabolic differences, and changes in protein structures that all act in unison to affect bacterial survival in the presence of antibiotics. The conversion of raw mass spectrometry outputs into clinical insights amenable to decision-making is dependent upon bioinformatics. Through the use of sophisticated computational methodologies, such as machine learning and network analysis, bioinformatics provides the means to incorporate heterogeneous omics datasets. Through this approach, resistance-related biomarkers can be identified, predictions for therapeutic efficacy can be made, and new targets for intervention can be identified. The intersection between high-resolution mass spectrometry, broad-based omic profiling, and strictbioinformatics provides a powerful platform for addressing the intractable challenge of antimicrobial resistance.

    Q.1.    How mastitis occurs in dogs? 
    Ans.    Mastitis in dogs initiates with the infection in the milk ducts of the dogs, where in the dog’s breast is enlarged as well as milk duct is blocked. The skin around the nipple might get cracked which might give a way to bacteria. In neglected cases it leads to cause gangrenous mastitis which is a severe form of mastitis in dogs.

    Q.2.    What are common pathogens responsible for mastitis in bitch?
    Ans.    Staphylococcus spp., Streptococcus spp. and Escherichia coli.
 
    Q.3.    What are the characteristic features of milk cytology during mastitis in bitch?
    Ans.    Milk cytology will show the presence of erythrocytes, degenerated neutrophils and bacterial agents.

    Q.4.    What characteristic physical changes of milk are seen during mastitis in bitch?
    Ans.    The viscosity of milk is increased and the colour of milk will be yellow to brown depending on the amount of blood and purulent exudates present.

Fill in the blanks
    Q.1.    ………… organisms are the common isolates from clinically affected quarters.
    Ans.    Coagulase-negative staphylococci
    Q.2.    ………….is the most common cause of mastitis in goats.
    Ans.    Staphylococcus aureus
    Q.3.    Streptococcal zooepidermicus causes …………. in does.
    Ans.    Chronic suppurative mastitis.
 
Write True/ False about the statements
    Q.1.    Pseudomonas organism causes an acute mastitis characterized by extensive necrosis and fatal septicemia.
    Ans.    True.
    Q.2.    In summer mastitis, non-lactating goats develop severe mastitis whereas lactating goats suffer from moderate degree.
    Ans.    False

    Q.1.    What is the cause of mastitis in non-lactating mare?
    Ans.    Mastitis may develop in a non-lactating mare due to abnormally high estrogen levels in her system. This excess female hormone can result from a pituitary gland tumor or consumption of high estrogen containing hay. High estrogen concentrations can cause mammary gland enlargement and trigger the production and secretion of milk, even though the mare is not pregnant and has not foaled. If the non-lactating mare begins to secrete milk due to excess estrogen, she may become a candidate for mastitis.

    Q.2.    Why equine mammary glands are less prone to mastitis than bovines?
    Ans.    The possible reasons are as follows:
    a)    Mammary glands are relatively smaller in size
    b)    Concealed position of the glands
    c)    Small milk capacity of the glands
    d)    Greater resistance to infection in equine mammary glands because of differences in endocrine environment and local immunity

Fill in the blanks

    Q.1.    ---------- and -------------- are the synonyms of mastitis of sheep.
    Ans.    Bluebag, garget
    Q.2.    In gangrenous mastitis, besides Staphylococcus aureus, ----------- organism may also be involved.
    Ans.    Clostridium perfringens A
Answer the followings

    Q.1.    What are the characteristic symptoms of mastitis in sheep?
    Ans.    The important symptoms include severe necrotizing inflammation of mammary gland, severe systemic reaction and reduced milk production
    Q.2.    Classify the sheep mastitis based on etiology and clinical symptoms.
    Ans.    a) Gangrenous mastitis by Staphylococcus aurcus mastitis b) Herd mastitis by Pasteurella hemolytica
    Q.3.    When the risk of mastitis in sheep is high?
    Ans.    During summer month between parturition and weaning

    Q.1.    The mastitis-metritis-agalactia (MMA) is a disease syndrome of which animal species? 
    Ans.    Swine

    Q.2.    What is MMA syndrome?
    Ans.    MMA syndrome is post parturient disease of sows and manifested as fever, anorexia, lethargy, agalactia, mastitis, swollen udder and appearance of vulvar discharge due to development of metritis.
 
    Q.3.    What is the critical time of occurrence of MMA in sows?
    Ans.    The mastitis-metritis-agalactia (MMA) syndrome occurs between 12-48 hrs of farrowing in sows.

    Q.4.    What is the probable causes for the development of MMA syndrome?
    Ans.    The probable causes of MMA syndrome are infectious mastitis, metritis, more feeding at pregnant period, nutritional deficiency, endocrine disorders and constipation.

Introduction

Measurement of pH of a solution and preparation of different types/strength of buffers are common practices in a laboratory, particularly in the Biochemistry and Molecular Biology laboratories. Therefore, in this chapter you will be acquainted with the procedures of measuring pH of a solution as well as preparation of some of the commonly used buffers in a laboratory.

Potential or Power of Hydrogen Ion (pH)

Measurement of pH of a solution is measuring the molar concentration of hydrogen ion [H+] in the solution, which is an indication of the acidity or basicity of the solution. The symbol pH stands for “power of hydrogen” and numerical value for pH is just the negative logarithm of the molar concentration of hydrogen ions [H+]. The ionic product of water (Kw ) is the basis for the pH scale. The total hydrogen ion concentration from all the sources and it can be experimentally measured.

The pH scale designates the concentration of H+ (and thus of OH−) in an aqueous solution, which ranges between 1.0 M H+ and 1.0 M OH−. The term pH is defined by the expression

Thus, the ionic product of water makes it possible to calculate the concentration of H+, with given concentration of OH−, and vice versa. The value of 7 for pH of a neutral solution is not an arbitrarily chosen figure; it is derived from the absolute value of the ionic product of water. A solution having pH greater than 7 is basic, and the concentration of OH− is greater than that of H+. A solution having a pH less than 7 is acidic.

3.1 MEASURES OF CENTRAL TENDENCY (AVERAGES)

When data are presented in raw form or in the form of frequency distribution, they fail to provide a general idea of the data under study. The general idea of statistical methods is to condense the raw data into a form that can convey some meaningful characteristic(s) of the data. Though classification simplifies the data and improves the understanding of the main characteristics in the data, it is still difficult to remember. In any distribution of data, values of a variable may tend to cluster (or concentrate) around a central value. The data must be condensed into a single value. Such presentation of data is called a Central Tendency and the measures devised to consider this tendency are known as Measures of Central Tendency.

An average condenses a frequency distribution or raw data and presents it in a single representative number. It is that single value which is considered as the most representative or typical value for a set of values. Such a value usually lies somewhere near the centre of the group. That is why, an average is usually referred to as a measure of central tendency. It is located at a point around which most of the other values tend to cluster and therefore it is also termed as a measure of central location.

8.1 NEED FOR ANIMAL DISEASE FREQUENCY MEASURES

The very essence of veterinary epidemiology is to measure animal disease occurrence and to make comparisons between the animal population groups. The animal disease frequency measures help us to understand the distribution of diseases in animal populations. Hence, the principal role of veterinary epidemiology is to describe and explain differences in the distribution of animal diseases between the populations.

Measures of the disease frequencies are used to describe how common an illness is concerning the size of the population (the population at risk) at a particular point in time. The major reason for determining the morbidity is to assess the extent of a disease problem in a population. This assessment is possible only when the number of diseased animals is calculated and compared with the total number of animals in the population. A report of 10 cases of mastitis in a dairy herd does not indicate the true extent of the disease problem, unless the report also indicates the total number of dairy animals in the herd or population. Thus, it is important to relate the number of cases of a disease to the population at risk of developing that disease.

Meat is animal flesh that is used as food. Most often, this means the skeletal muscle and associated fat, but it may also describe other edible tissues such as organs, liver, brain, bone marrow, kidney, or lung. Meat is a nutrient dense food. Meat contains protein, fat, enzymes, vitamins and minerals for proper human nutrition. Goats are important producer of meat besides milk, fiber and skin. Goat meat is lower in calories, total fat, saturated fat and cholesterol, than traditional meats.Goat have number of characteristics , such as high reproductive potential , short generation interval , ability to thrive on shrubs, bushes and tree leaves, high digestive efficiency for cellulose and less susceptibility to infectious diseases, that make them suitable as meat producing livestock. Since these animals are more concentrated in the developing part of the world, they play a major role in subsistence agriculture of developing economies of poor and marginal farmers inhabiting such countries. More than 95 % of goats are concentrated in dry tropical and subtropical areas of poor agricultural potential and even on marginal lands of the world (Morand Fehr and Boyazoglu, 1999). Goat has several other advantages as producer of meat which make it predominant and preferred animal for meat production. Often because of the above mentioned reason, goat meat is sold two to three times costlier as compared to pork in India. Beef is unavailable in India with the exception of Kerala and West Bengal. Goat meat has an edge over mutton too, as the number of goats slaughtered for meat purposes (40%) far exceeds the number of sheep slaughtered for similar purposes. The slaughter rate of goat is at the level of 39.7 % as compared to 31.8% for sheep and 11% for buffaloes, respectively. Sheep and goat meat are both called “mutton” in India. Goats convert the poor quality roughage, browse tree leaves, which would have otherwise gone waste, into valuable source of protein in several desert or hostile areas of the world where other classes of livestock like cattle and buffalo will starve to death. Goat meat production and consumption has an important place in the economy of Asian and African countries. Goats have been recognized as an important source of meat in the rural areas of these countries. Commercial meat-goat enterprises account for majority of the meat-goat production systems and herd sizes range from five to several thousand does. Prolificacy, milk production and pre-weaning growth are important in achieving good productivity of commercial meat production enterprises.