eChapters

Introduction
The advent of antibiotics, beginning with penicillin in 1941, transformed medicine by rendering bacterial infections treatable [1]. However, antimicrobial resistance (AMR) has emerged as a profound threat, enabling bacteria to survive in the presence of drugs designed to inhibit or kill them and these are rooted in ancient microbial ecosystems evidenced by resistome genes in 30,000-year-old permafrost [2]. AMR has been exacerbated by human practices: approximately 50% of antibiotic prescriptions are inappropriate, 70% of global antibiotics fuel agriculture, and environmental pollution creates resistance hotspots [1]. By 2025, AMR directly causes 1.3 million deaths annually, with economic losses projected at $100 trillion by 2050 [1]. Priority pathogens, including carbapenem-resistant Enterobacteriaceae (CRE), methicillin-resistant Staphylococcus aureus (MRSA), and colistin-resistant strains via mcr-1, challenge last-resort therapies, particularly in low-resource settings [3]. This chapter delivers a technically extraordinary exploration of AMR, structured into genetic, biochemical, and evolutionary mechanisms, each elaborated with molecular precision and step-by-step clarity for learners. Genetic alterations provide the DNA-level foundation, biochemical pathways execute molecular defenses, and evolutionary forces drive adaptation and dissemination. It ensures scientific rigor and broad appeal for students, researchers, clinicians, and policymakers.
Historical Context and Global Burden
AMR emerged shortly after antibiotics’ introduction, with penicillin-resistant S. aureus identified by 1942 due to ß-lactamase production [1]. The 1960s

Medication refers to administration of drugs. The route, dose and frequency of the drugs administration is most important in providing health care and welfare of the animals. Wide range of body size and their weight in animals and rearing practice makes the treatment and drug administration strategies a bit complex. Because of this complexity wide range of drug formulary and drug delivery strategies has been developed in animals for health care. Route of drug administration varies from oral administration, parenteral administration to local application on skin and mucus membrane. Similarly forms of the drugs varies from liquids, semisolids to solids for oral administration; from clear solution, suspension, emulsion to powder for parenteral administration; and from powder, cream, paste, suspension, ointment, solutions to emulsifiable concentrates for local application. Oral Administration of Drugs All three forms of drugs viz. liquids (solution, emulsion, suspension), semisolids (paste), and solids

It is more common in dogs and frequently seen in the skin and oral cavity. It usually occurs in 5 to 11 years old dogs. Benign melanomas are solitary, circumscribed, alopecic firm and freely movable blue black coloured nodules in the skin. Malignant melanoma is sessile, polypoid to plaque like grey or brown black coloured granules in the skin with ulceration.

Cabbage semilooper: Thysanoplusia orichalcea :Noctuidae

Eggs are laid singly on lower surface of leaves.  The emerging larvae feed as a scraper initially and then feed voraciously on the leaves. The larva is greenish and hence not easily noticed initially. But when patches of damage occur in the crop, examine for the larvae and remove and destroy all the larvae. Chemical control is not advisable, and spraying extracts of other aromatic plants can deter egg laying very well.

ìMetabolic Diseasesî mean the diseases that occur due to the ìimbalance between the rate of input of dietary nutrients and the ìoutput of productsî (product like new born, milk for young one etc.) and lead to the occurrence of metabolic derangements in the affected wild animal species.

Metabolic diseases assume the greatest significance in dairy cows and buffaloes during transition period, and occur due to imbalance in input and
output of production. The main reasons of occurrence of metabolic diseases are an imbalance between the rate of ‘input’ of dietary nutrients and ‘output’
of production.

1 Metabolic profile test to know the nutritional status of a dairy animal, by assessing the blood used components

a) Complete profile test b) Metabolic profile test

c) Compton metabolic profile test d) Dairy animal metabolism test

Key Points

• Electrolyte Imbalance: Regularly monitor and rectify imbalances in potassium, sodium, and other electrolytes dependent on the individual situation. Prompt management requires early detection of metabolic emergencies.

• Fluid Management: To restore intravascular volume and prevent dehydration in many metabolic emergencies, adequate fluid replacement is essential.

• Continuous monitoring of vital signs, laboratory values, and clinical status is essential for evaluating the efficacy of interventions and making necessary modifications.

Introduction

A class of disorders known as "metabolic emergencies" affect metabolism and physiology abnormally in both congenital and acquired forms. Among these ailments are endocrine disorders, thyrotoxicosis, inborn metabolic inefficiencies, and illnesses linked to electrolyte imbalances. Hypercalcemia, hyponatremia, hypoglycemia, hyperglycemia, and diabetic ketoacidosis, as well as hyperuricemia, hyperuremia, and hypercreatininemia due to renal failure, lactic acidosis, hyperammoniemia, and adrenal failure, are metabolic emergencies that pose a life-threatening risk.

Metabolic profile testing (MPT) refers to the use of a battery of tests for the diagnosis of subclinical nutritional and metabolic disease in dairy cows on a herd basis. Typically, blood samples from 5-7 animals per production group are tested for various analytes. MPT is most useful in the diagnosis and management of periparturient disease. Specific nutrient imbalances, either deficiency or excess, in the diet of late-gestation cows have been related to increased prevalence of milk fever, hypomagnesemia, retained placentas, downer cow syndrome, mastitis, udder edema, ketosis, hepatic lipidosis, and displaced abomasums.

Introduction
Metabolism encompasses a series of cellular reactions essential for sustaining life in living organisms. These reactions are part of interconnected pathways within cells, ultimately providing the necessary energy for cellular functions. The importance and evolutionary benefits of these pathways are evident as they are conserved across animals, plants, fungi, and bacteria. In eukaryotes, metabolic pathways operate within the cytosol and
mitochondria, with glucose and fatty acids being the primary sources of cellular energy in animals. Metabolism is organized into distinct pathways to optimize energy capture or minimize energy expenditure. It can be divided into two main processes: anabolism, which involves the synthesis of complex macromolecules, and catabolism, which involves their degradation.
Carbohydrates are the major source of energy in the animal’s diet. Absorbed glucose could be utilized by the animal for several functions. The first priority is the formation of glycogen in the liver, which is stored in muscle and hepatic tissue. However, the body stores very little as glycogen (a starch-like compound), and glycogen could be rapidly hydrolyzed back to glucose through a process called glycogenolysis. This helps in maintaining blood
glucose at a narrow range in normal healthy animals. The second priority is oxidation to form energy (a major function) and is described in the next sections. Since glycogen storage is limited, excess glucose is converted to fat and is stored in adipose tissue. This is accomplished by the breakdown of glucose to pyruvate through a process called glycolysis, which is then available for fat synthesis. The catabolism of glucose occurs in two metabolic pathways: glycolysis and the tricarboxylic acid (TCA; also called citric acid or Kreb’s) cycle.
Glycolysis: Enzymes for glycolysis are located in the cytosol of the cell, and glycolysis occurs in this part of the cell. Glycolysis is the breakdown of 6 C glucose into two 3 C end product pyruvates in aerobic metabolism and lactic acid in anaerobic metabolism. It is a catabolic pathway involving oxidation and yields ATP and NADH (reduced NAD) energy. Glycolysis is the pathway by which other sugars (e.g., fructose, galactose) are catabolized by converting them to intermediates of glycolysis. Fructose can be converted to fructose-6- phosphate by hexokinase. Galactose can enter glycolysis by being converted to galactose- 1-phosphate followed by conversion (ultimately) to glucose-1-phosphate and subsequently to glucose-6-phosphate (G6P), which is a glycolysis intermediate.

Key Points

• It is frequently reported that metal, non-metal, and metalloid components cause toxicity in farm animals.

• The type of element causing toxicity affects both the treatment and the clinical indications of toxicity.

• The best course of action for managing toxicity is to treat symptoms f irst, then, if the causal toxin is known, use targeted antidotes.

Introduction

Animal diets contain a variety of environmental components, including metals and non-metals. Certain substances are necessary for the animal and only become poisonous when consumed in excess, while others simply endanger the animal's health. However, a number of variables, including the absorbed dose, the exposure route, the period of exposure (acute versus chronic), and others, affect how toxic the substance is, and these variables must be taken into account when diagnosing, treating, and managing clinical cases. Given the multitude of factors affecting these metals' toxicity. An attempt is made in this chapter to present a streamlined synopsis of the toxicity of metals, non-metals, and metalloid elements that are frequently encountered.

Metritis is a condition seen in cattle in which there is the inflammation of the entire thickness of uterine wall (endometrium, myometrium and serosa). When it occurs just after parturition, it is called puerperal metritis. It usually occurs after abnormal birth like in case of dystocia, abortion, RFM etc. The animal shows systemic signs like increase in temperature initially, increase in respiration rate, anorexia, toxemia etc. There is expulsion of large volume of foul smelling reddish to brown discharge from vagina frequently associated with straining. The expelled discharge from vagina is often with pieces of degenerating placenta.

Introduction

Pastoralism involving rearing of different livestock species makes significant contributions to the economy of developing countries, both in terms of providing employment and income opportunities and in supplying nutrition to rural poor. Pastoralism generally has a mobile aspect, and movement of animal herds in search of fresh pastures and water is a key feature qualifying pastoralism. The term nomadic is used when mobility is high and in irregular patterns; transhumant when there are regular back-and-forth movements between relatively fixed locations; and sedentary for the rest. Pastoralists inhabit zones where the potential for crop cultivation is limited due to steep terrain, extreme temperatures and low and highly variable rainfall. Within this unpredictable, vulnerable and dynamic environment, they have developed successful mechanisms of adaptation to maintain an ecological balance between themselves and the natural environment. Thus, pastoralism is a successful strategy to support a population on less productive land that adapts well to the environment. Transhumance is the seasonal migration of livestock and pastoralists between higher and lower pastures.

Background
Milk is known as a whole food for human consumption. It is the first food of the newly born human mammals. Milk contains almost all the nutrients required for the patients, the elderly, pregnant mother and new born baby. There is no doubt that milk plays a significant role in human development. We can prepare many physical forms of milk like cheese, yogurt, ghee, and milk powder. The calcium content is higher in buffalo than in cows. Buffaloes are the second largest source of milk and produce 15 % milk. Higher biological value proteins, fat, soluble vitamins, fatty acids, amino acids, water-soluble vitamins, and calcium are all present in milk. The main antioxidant components of milk and milk products are sulfur, phosphate, zinc, and amino acids like cysteine (necessary to produce collagen).
Milk somatic cells are normal cells found in milk in small amounts (epithelial cells), some of which are leukocytes (white blood cells). When mastitis is infected or injured, leukocyte production rises. Milk with a low SC level produces superior milk products that last longer. Better milk management, better cleanliness, and correct nutrition all contribute to a decrease in milk SC, which indicates poor milk production hygiene and a cause of mastitis. Milk somatic cells are made up of both immunological and milk-producing cells. In terms of defense, somatic cells are the second line. During the routine process of milking, these cells are secreted in milk. Somatic cell count is a good tool for evaluating farm cleanliness in bulk milk and is utilized worldwide as an indication of udder health in individual milk. The most prevalent condition affecting dairy buffalo production and their health and well-being is mastitis. The somatic cell count consists of immune cells (75%), such as neutrophils, macrophages, lymphocytes, erythrocytes, and epithelial cells (25%). Investigators have made different attempts to exploit milk somatic cells as a potential non-invasive sample to diagnose early pregnancy udder health in bovines.

The goat milk has several peculiarities, being a food of high digestibility and high nutritional value.Goats can hardly compete with cattle or buffalo in milk production ability. Goats produce approximately 2% of the world’s total annual milk supply. Neverthless, goat is an important source of animal protein for the landless and marginal farmers of tropical countreis, in areas where other classes of livestook cannot possibly survive and for several classes of people allergic to cow milk. Goats contribute about 4 % of the total milk produced in India. Goats and sheep producing milk for people start with the beginnings of domestication. For thousands of years they have provided sustainance for the people. During the last 150 years genetic selection and better feeding conditions led to several superior goat and sheep breeds in some countries in terms of milk and solids productivity, proving a great potential for evolution in both species, and responding to an increasing market demand and popularity. Some sheep breeds can produce more than 1000 kg milk in one lactation and several goat breeds more than 2000 kg milk, but in physiologically comparable terms of 4% fat-corrected milk both species can be equal. Goat milk production leaders have been capable of producing 10 kg milk per day of lactation.

Goat has been referred to as the Poor mans cow or wet nurse in Europe. Goat provides the cheapest source of milk to the poor both in rural and urban areas. The milk of goats is sweet, nourishing and medicinal. Goat milk is white in colour. Goat milk is whiter than whole cow milk. Butter and cheese made from goat milk are also white, but may be colored during processing. Exploitation of goats as a source of milk for human beings dates back from antiquity. Goat milk has been acknowledged as an ideal food for the aged, the sick and convalescent as well as for infants and growing children. Ever since Cleopatra filled her bathtub with goat milk , fresh goat’s milk has been revered for its superior moisturizing properties.

CALCIUM & PHOSPHORUS

Source of mineral

Soluble grits (Limestone grits containing chiefly CaCo3) which is soluble in acid medium of crop. It differs from insoluble grits (gravel, granites, sand etc.) which acts only mechanically in the gizzard.

1 Deficiency of which of the following trace minerals leads to parakeratosis of skin in pigs

a) Zinc b) Copper

c) Cobalt d) Iron

1 Goose stepping gait is observed due to deficiency of

a) Biotin b) Pyrodoxin

c) Pantothenic acid d) Niacian

1 The system that is most susceptible to hypoxia is

a) Respiratory System b) Cardiovascular System

c) Nervous system d) Digestive system

1 The focal point of any disease investigation in animal is making of

a) Treatment b) Diagnosis

c) Management d) Prognosis