eChapters

Essential oil is a concentrated hydrophobic oily fragrance liquid extracted from plants, containing easily evapourated chemical compounds at room temperature. Essential oils are extracted from the different plant parts including the flowers, leaves, stem, bark, and roots, and they are extracted from different techniques. The most preferable method of extraction is hydro distillation which is easy to use and cheap. Essential oils are used in every field of life, used in aromatherapy, and act as antifungal, antioxidant, anxiety, pain relievers, and depression.

Introduction
Antimicrobial resistance (AMR) has emerged as one of the most alarming public health challenges of the 21st century, threatening the effectiveness of life-saving drugs and the future of modern medicine. This crisis is not limited to healthcare settings; it spans communities, agriculture, and the environment, impacting every facet of society. India, with its large population, high burden of infectious diseases, unregulated access to antibiotics, and considerable pharmaceutical manufacturing industry, stands at the forefront of the global struggle against AMR. The rise of resistant microbes stems from a complex interplay of factors: overuse and misuse of antibiotics in humans and animals, poor infection prevention practices, lack of sanitation, and environmental contamination from antibiotic waste. As a result, common infections are becoming harder to treat, surgical procedures riskier, and healthcare costs are escalating, all leading to increased morbidity and mortality. Tackling AMR requires robust intersectoral collaboration—no single stakeholder can succeed alone. Non-governmental organizations (NGOs), industries, and Corporate Social Responsibility (CSR) initiatives form a critical triad in India’s fight against AMR. NGOs drive grassroots education, advocacy, surveillance, and policy change, often bridging gaps where governmental capacity is limited. The pharmaceutical and healthcare industries, responsible for antibiotic manufacturing, distribution, stewardship, and infection control, play a pivotal role in curbing environmental emissions, supporting responsible use, and promoting the discovery of new treatments. Meanwhile, CSR channels the resources and expertise of corporations towards sustainable, communityfacing projects that promote awareness, improve hygiene, foster stewardship, and drive systemic change.

To fully explore the history of medical and drug development, it would be necessary to go back as early as 13,000 BCE, where drawings on the Lascaux caves in France suggest that humans were using plants for medicinal purposes. Since then, medical traditions have evolved and dissolved across societies all over the globe. Natural products including plants, animals and minerals have been the basis of treatment of human diseases. The last two centuries have seen a focus on scientific medicine, with a focus on drugs whose effects that have been proved by the scientific method. Yet given this setting, many of the major pharmaceutical corporations have renewed their strategies in favor of natural product drug discovery. Numerous drugs have entered the international pharmacopoeia via the study of ethnopharmacology and traditional medicine. At this point in history, it is possible for both traditional and western medicine to benefit from collaboration between the two disciplines. For traditional medicines, newer guidelines of standardization, manufacture and quality control and scientifically rigorous research on the scientific basis for traditional treatments will be required. In return, traditional medical traditions can offer a more holistic approach to drug design and myriad possible targets for scientific analysis. Powerful new technologies such as automated separation techniques, high-throughput screening and combinatorial chemistry are revolutionizing drug discovery. Traditional knowledge can serve as powerful search engine, which will greatly facilitate intentional, focused and safe natural product drug discovery and help to rediscover the drug discovery process. By looking at the historical trends in drug and medical developments, it is possible to understand how current drug development will benefit from this partnership.

AMR is undoubtedly the most serious challenge ever faced by the world health community because of its multifaceted complex nature and its overwhelming impact over the human and veterinary communities. Livestock sector is no exception to have the serious and devastating impact from the emergence and spread of AMR. Because of a steady shift towards intensive farming and increased stocking density there has been increase occurrence of infectious diseases. Growing world population, rapid urbanization, and deforestation, shrinkage of grazing land all aided to this process. This leads to increased usage of antimicrobials in food animals – cause of concern for the scientific community as AMU is known the single most important driver of AMR.

Rodents and lagomorphs are two distinct groups of mammals, often mistaken for each other due to some shared physical characteristics. However, they belong to different taxonomic orders and possess several key differences

5.1. Rodents

They belong to the order Rodentia and the example species include rats, mice, squirrels, hamsters, guinea pigs, beavers, and porcupines. Order Rodentia is the largest and most diversified mammalian group, differing widely in size, behavior, feeding habits, anatomy, and physiology. Based on the anatomy and functional differences of masseter muscles, it is divided into 3 suborders viz., Caviomorpha, Myomorpha, and Sciuromorpha. Caviomorpha is also called hystrichomorpha and includes guinea pigs, chinchillas and degu. The suborder Myomorpha includes mice, rats, hamsters and gerbils. These animals have the strongest ability to gnaw among animals in all 3 suborders. Sciuridae is a family that includes small or medium size rodents. This family consists of tree squirrels, ground or medium size squirrels, and large size squirrels also known as marmots or prairie dogs (Cynomys ludovicianus). Smaller and less bushy tailed squirrels are known as chipmunks (Tamias striatus) and flying squirrels (Glaucomys volans).

Cytauxzoonosis is an emerging tick-borne disease of domestic cats and wild felids caused by the protozoa of the genus Cytauxzoon. The common clinical findings in affected cats are similar to other hemoparasitic infections namely anorexia, depression, anemia, vomiting, icterus, and high fever. The first reporting of feline cytauxzoonosis with four fatal cases was done by Wagner in Missouri, USA in the year 1976.

Bobcats (Lynx rufus) are recognized as the natural wild reservoir of Cytauxzoon felis with prevalence reaching up to 100% in some enzootic areas (Zieman et al., 2017). This infection is highly fatal in domestic cats with 97% mortality without treatment (Meinkoth and Kocan, 2005). However, subclinical infection in some cats has also been evidenced i.e., they were infected with C? felis but did not display the acute clinical signs of the disease before diagnosis (Brown et al., 2010 and Rizzi et al., 2015).

Several other wild felids apart from bobcats and domestic cats have been found to be affected by fatal or nonfatal Cytauxzoon sp. infection. These include lions, jaguar, cougars, pumas, ocelots, Iberian lynx, meerkats, European wild cats and Asiatic wild cat, panthers, and tigers (Jakob and Wesemeier, 1996; Rotstein et al., 1999; Yabsley et al., 2006; Andre et al., 2009; Filoni et al., 2012 and Furtado et al., 2017).

7.1. Introduction

Chemical restraint of insectivores is a technique used by veterinarians and wildlife biologists to immobilize small mammals that primarily eat insects, such as shrews, moles, and hedgehogs. It is essential for performing examinations, administering medications, and conducting research on these animals.

There are several factors to consider when chemically restraining insectivores:

• Species: Different species of insectivores may have varying degrees of sensitivity to different drugs.

• Age and health: Younger and healthier animals generally tolerate chemical restraint better than older or sick animals.

• Temperature: Insectivores are sensitive to temperature regulation, so it’s crucial to maintain a stable body temperature during restraint.

Definition 

Radiation poisoning, also called “radiation sickness” or a “creeping dose” is a form of damage to organ tissue due to excessive exposure to  radiation. The term is generally used to refer to acute problems caused by a large dosage of radiation in a short period, though this also has occurred with long term exposure to low level radiation. Many of the symptoms of radiation poisoning occur as ionizing radiation interferes with cell division.

The clinical name for “radiation sickness” is acute radiation syndrome . A short exposure can result in acute radiation syndrome; chronic radiation syndrome requires a prolonged high level of exposure. A chronic radiation syndrome does exist but is very uncommon; 

Renal failure is one of the most commonly presented conditions in veterinary clinics world over. Although the potential for recovery is favorable, most animals die due to multisystemic effects induced by azotemia. Hence, extracorporeal removal of endogenous and exogenous toxins is very essential for expanding the window of recovery. Hemodialysis has been used in treatment of veterinary patients for the last 50 years and is still one of the most expensive and technically demanding treatment modalities in veterinary medicine. It is an advanced extracorporeal renal replacement therapy which has recently transitioned from a clinical curiosity to a more advanced standard for management of acute kidney injury (AKI) and chronic kidney disease (CKD) in companion animals particularly dogs and cats.

In addition to eliminating selective toxins that cause AKI, regardless of renal function, hemodialysis is also effective in the removal of pharmacokinetically active soluble uremic substances in CKD to enhance life expectancy and quality of life (Singh, 2021). Other common indications of hemodialysis in veterinary medicine include fluid overload, dysregulated body fluid composition, drug overdose or intoxications (Foster, 2020; Singh et al?, 2024). Medical management alone is often insufficient to control the clinical signs and metabolic abnormalities associated with uremia when AKI is severe or when there is involvement of acute on chronic kidney disease. Consequently, animals are at risk of death within a short therapeutic window, despite the potential reversibility of AKI and the improvement of life expectancy in CKD.

Introduction

As India is the leading global economy with livestock contributing 4.66% in total GVA to Indian Economy (PIB, 2024) and ranks 1st in Milk Production, 2nd in Egg production, 5th in meat production (BAHS, 2024). Being the largest exporter of milk and meat in the world , this has led to the intensification of animal production due to the increasing demand of major livestock products (MLPs), which has led to an increasing overall use of antimicrobials. Antimicrobials are invariably used to preserve animal and public health, but also as growth promoters at a sub-therapeutic level. The overuse and misuse of antibiotics can contribute to antimicrobial resistance. The growing resistance to antimicrobial drugs could reverse the therapeutic benefits; animals’ resistance to antimicrobial drugs, reduces productivity and leads to economic losses. Unwanted antimicrobials residues may be present in products of animal origins, in animal waste contaminating soil and water and the environment. Seventy-five to ninety percent of antimicrobials used in livestock are excreted, mostly unmetabolized. AMR poses a serious threat to the safety and quality of animal origin food, food security and livelihoods. Unhealthy and unproductive animals are no longer able to generate food products of acceptable safety and quality for human consumption and can no longer contribute to income generation. Insufficient standards for residues in animal food products, reduces the livestock sectors potential of access to trade and increases public health risks (https://www.fao.org/antimicrobial-resistance/key-sectors/animal-production/ en/).

Principle

Good documentation constitutes an essential part of the quality assurance system. Clearly written documentation prevents errors from spoken communication and permits tracing of batch history. Specifications, Manufacturing Formulae and instructions, procedures, and records must be free from errors and available in writing. The legibility of documents is of paramount importance.

General

Specifications describe in detail the requirements with which the products or materials used or obtained during manufacture have to conform. They serve as a basis for quality evaluation. Manufacturing Formulae, Processing and Packaging Instructions state all the starting materials used and lay down all processing and packaging operations.

Procedures give directions for performing certain operations e.g. cleaning, clothing, environmental control, sampling, testing, and equipment operations.

Records provide a history of each batch of product, including its distribution, and also of all other relevant circumstances pertinent for the quality of the final product.

ABSTRACT

Phospholipase A₂ catalyzes the hydrolysis of glycerophospholipids at the sn-2 position and releases fatty acids and lysophospholipids. When the fatty acid is the arachidonic acid, a complementary metabolism leads to pro-inflammatory mediators such as prostaglandins, leukotrienes, thromboxanes and platelet activating factors. Thus, modulating pro-inflammatory lipid mediator production by inhibiting PLA₂ activity remains a potential target for the development of new drugs for the treatment of inflammatory diseases. Medicinal plants have been explored therapeutically in traditional medicines and are a valuable source for drug discovery. Molecular docking studies were carried out for anti-inflammatory compounds from various plant sources with human non-pancreatic secretory phospholipase A₂.

Introduction
The major conditions described under this heading are as follows :
    1.    Snakebite 
    2.    Scorpion bite 
    3.    Black widow spider sting 
    4.    Toad poisoning

Snake Bite
Poisonous snakes mainly belong to three categories

Tumour in Domestic Animals
Tumours in domestic animals, much like in humans, can occur in various tissues and organs. These can range from benign growths (non-cancerous) to malignant tumors or cancerous. Their causes, types, diagnosis, and treatments depend on the animal species, breed, age, and environmental factors. Common tumours in dogs are mammary tumours, skin tumours, lymphoma, osteosarcoma and hemangiosarcoma. In cats lymphoma, squamous cell carcinoma, mammary tumors and fibrosarcomas are reported. In cattle, squamous cell carcinoma, lymphosarcoma and papilloma are common. In horses, sarcoids, melanomas and squamous cell carcinoma are common. In small ruminants (sheep and goats) lymphoma and skin tumours are reported. In pigs, lymphoma and melanomas are commonly reported. Diagnosis of cancers are done by physical examination, cytology, biopsy, imaging: X-rays, ultrasound, CT, MRI and blood Tests.

Medicinal Plants for Arsenic Toxicity
Name of the Condition- Nephrotoxicity
About the condition/Definition- Intoxication of arsenic (arsenite or arsenate) result in renal impairment which is marked by a significant increase in the levels of creatinine, urea, uric acid and decline in levels of plasma protein profile especially albumin level in body.
Etiology- Nephrotoxicity results from arsenic damages mitochondria, which results in the excessive production of reactive oxygen species (ROS), a decline in ATP, and a suppression of respiratory activities along with dramatic alterations in the incidence of cellular growth. There is acute inflammation and the development of tubular necrosis, which lowers the glomerular filtration rate (GFR).
Symptoms- Phosphaturia (Fanconi syndrome), aminoaciduria, proteinuria and glycosuria along with progressive drop of renal function.
Pathogenesis- Arsenic produces renal damage by reducing mitochondrial complex function and elevating cellular ROS levels which directly damages renal cells. Membrane lipid peroxidation and protein oxidation by ROS, which results in complete loss of membrane potential and mitochondrial swelling.
General treatment- Acute arsenic intoxication can be counter by using chelating agents like DMSA, DMPS and BAL, fluid therapy, forced dieresis & dialysis.

Introduction
Metal-catalyzed C–N cross-coupling reactions are powerful synthetic methods for producing both simple and complex organic compounds, including natural products. These methodologies typically involve shorter reaction times and can achieve good to excellent yields with a broad range of reactions, exhibiting high regioselectivity in certain cases. The coupling of carbon and nitrogen to create organic compounds that contain nitrogen, such as urea and amides, is one of the most fundamental chemical reactions and plays a crucial role in human civilization. C–N coupling reactions have significant importance in industry, starting with the production of ammonia (NH3) through the Haber–Bosch process. Molecules containing C–N bonds are prevalent in a variety of organic materials, natural products, pharmaceutical compounds, and agricultural chemicals. This wide applicability makes them crucial chemicals, leading synthetic organic chemists to continuously seek out novel synthetic methods. In recent years, numerous synthetic strategies involving either transition metals or metal-free enzymes and biocatalysts have emerged. Additionally, new technologies, such as electroreduction, have been employed in these C–N coupling processes (Bariwal & Van der Eycken, 2013, Li et al., 2022, Yin et al., 2024, Rahman et al., 2022).
Common copper salts/complexes, and diamino ligands
Copper is commonly found coordinated by two, three, or four ligands, resulting in linear, trigonal planar, or tetrahedral geometries. Well-known and inexpensive copper halides, such as copper (II) glycinate, copper (II) acetylacetonate (particularly CuI), copper acetates, copper oxide, copper sulfate, and copper triflate salts, are widely used as catalytic systems in C–N bond-forming reactions. Additionally, other complexes such as Cu di-isopropyl salicylate (CuDIPS), copper phthalocyanine, and copper aspirinates have also been employed in these reactions. Copper-catalyzed azide-alkyne cycloaddition (CuAAC) reactions are well-established methods for forming triazoles via C–N bonds in one pot. These reactions often involve domino click chemistry and green approach methodologies, leading to the synthesis of biologically important triazoles. Copper salts in combination with diamine ligands are widely used in C–N bond-forming reactions through cross-coupling strategies. Some notable examples of such diamino compounds that can coordinate with copper are illustrated in Figure 1.

1. Introduction 
Among the innumerable gifts of nature, plants, animals and minerals have been the basis of treatment of human diseases. Generally it is either of prebiotic origin or originating from microbes, plants or animal sources. More likely they are the natural expression of the increase in complexity of organisms. Beside animals, plants are one of the most important sources of natural products. From the very beginning of their existence, man has acquainted himself with plants and used them in a variety of ways throughout the ages. In search of food and to cope successfully with human sufferings, ancient man began to distinguish those plants suitable for nutritional purposes from others with definitive therapeutic action. Some plants are widely used as food while others showed beneficial effects against various human sufferings such as injuries and diseases. This relationship has grown between plants and man, and many plants have come to be used as drugs. The use of plant as a drug can be divided broadly into five periods. The early period covers the Indian, Chinese, Sumerian, Egyptian and Assyrian civilizations followed by the Greco-Roman, Arabian, Medieval and modern periods (Buchnman, 1980). Among ancient civilisations, India has been known to be rich repository of medicinal plants as the forest in India is the principal repository of large number of medicinal and aromatic plants, which are largely collected as raw materials for manufacture of drugs and perfumery products. About 8,000 herbal remedies have been codified in Ayurveda. The Rigveda (5000 BC) has recorded 67 medicinal plants (Chopra et al., 1956), Yajurveda 81 species and Atharvaveda (4500-2500 BC) 290 species. Charak Samhita (700 BC) and Sushrut Samhita (200 BC) had described properties and uses of 1100 and 1270 species, respectively (Kirtikar & Basu, 1918; Baquar, 1989) in compounding of drugs and these are still used in the classical formulations, in the Ayurvedic system of medicine. Before the availability of synthetic drugs, medicinal plants were used as the most primary source of medicine and continue to provide the mankind with new remedies for many diseases. These were used in crude forms like expressed juice, powder, decoction or infusion. As the plant have specialized biochemical capabilities they are able to synthesize and accumulate a vast array of primary and secondary chemical constituents or phytochemicals (Ghazanfar, 1994). Phytochemicals are responsible for metabolic activities and used for defense purpose. They are produced by specific biochemical pathways, which occur inside the plant cells. Due to their physical characteristic and chemical properties these chemical constituents are highly beneficial to the mankind and most importantly in primary health care. The first chemical substance isolated from plants was benzoic acid (Figure 1) discovered in 1560. The potent pain-killer, morphine (Figure 1) was separated from the dried latex of Papaver somniferum L. (opium) by F. W Serturner (1783-1814). The bioactive molecule, ephedrine (Figure 1) is one of the most potent plant based medicine and was isolated from Ephedra sinica (Ma huang). It was used in the form of various salts to combat bronchial asthma (Tang & Eisenbrand, 1992). Reserpine (Figure 1) an indole alkaloid, obtained from the roots of Indian plant, Rauwolfia serpentina, was used to treat hypertension. Taxol (Figure 1) is a diterpenoid first isolated from the stem bark of pacific Yew, Taxus brevifolia and used against ovarian for the treatment of ovarian cancer.

1. Introduction
Organic polymers are large, chain-like molecules made up of repeating structural units (monomers) that are primarily carbon-based. These polymers are found both in nature and can be synthesized in labs, and they form the basis of many materials we use daily. Here are some main types of organic polymers (Harris, F. W., 1981).
2. Natural Organic Polymers
These are polymers found in nature. Examples include:
• Proteins: Polymers made up of amino acid monomers, serving as the building blocks for tissues and enzymes in organisms.
• Nucleic Acids: DNA and RNA, which are composed of nucleotide monomers, store genetic information in cells.
• Polysaccharides: Carbohydrate polymers like cellulose (found in plant cell walls), starch (energy storage in plants), and glycogen (energy storage in animals).
• Natural Rubber: Derived from latex in plants, primarily from the rubber tree (Hevea brasiliensis).
3. Synthetic Organic Polymers
These are human-made polymers produced through chemical synthesis.
Common examples include:
• Polyethylene: A plastic used in bags and containers, made from ethylene monomers.
• Polystyrene: Used in packaging, insulation, and disposable cutlery, made from styrene monomers.
• Polyvinyl Chloride (PVC): A durable polymer used in pipes, cables, and construction, made from vinyl chloride monomers.
• Nylon and Polyester: Common synthetic fi bers used in textiles.

Introduction

A variety of glassware, plasticware, and other apparatus are used for experimental purposes in a laboratory. They need to be washed, cleaned, dried, and sometimes sterilized before they can be used in the next set of experiments. Glassware needs special care of handling during washing, drying, sterilization, etc. because even a minor mistake in handling of glassware may result in its cracking/breakage and it may become unserviceable. All of these may result in a delay in experimentation if spare piece of the glassware is not available or it may affect serviceability of the associated apparatus.

A large number of glassware such as beaker, flask, measuring cylinder, burette, test tube, etc. are regularly used in a laboratory, which requires careful handling during the different stages of experiment as well as after completion of the experiment while preparing them for another set of experimentation.

Glass being a complex silicate, its properties depends on the type of silicate anions in the structure and the cations formed. Thermal properties of glass changes by the addition of boron oxide (B2 O3 ); hence, borosilicate glass is extensively used in the laboratories. With this brief background information, let us discuss about the necessity, procedures, and precautions during washing, cleaning, drying and sterilization of laboratory-wares. Figure 3.1 shows various advantages of using borosilicate glass in a laboratory.

Good laboratory practices require clean glassware, because an experiment may give erroneous result if dirty glassware is used at any step of the experiment. Hence, the glassware must be physically and chemically clean; and in many cases, it must be microbiologically clean or sterile. The commonly used glassware in a laboratory include test tube, measuring cylinder, pipettes, beakers, flasks, microscopic slides, etc.