eChapters

Recent pandemic (COVID-19) impacted global economy significantly and increased economic disparity. Vaccines are significantly beneficial and cost effective compared to other public healthcare preventives. Vaccines reduce hospitalisation, treatment costs and associated morbidity and mortality. Although COVID-19 vaccination and natural viral infection protected against reinfection, it waned in part due to emerging novel variants. Waning immunity from vaccine and natural infection are documented, and it was more so in severe hospitalised COVID-19. Many countries developed their own vaccines successfully to counter as COVID-19 became global. Vaccines needed updates to effectively fight COVID-19 as the wild strain constantly mutated. Among the intrinsic, perinatal, extrinsic, environmental, behavioural and nutritional aspects, the host factors included antibiotics usage pattern, sleep habits, physical activities, smoking habit and alcohol intake. Similarly, the external factors influencing vaccine response included childhood vaccination, early-age infections, nutrient supplements soon after birth, and breastfeeding pattern. Vaccination time also affected the efficacy. Forenoon vaccination developed less breakthrough infection compared to afternoon or evening vaccination due to varying immunological responses like immune cell functions, recruitment and transport, owing to circadian rhythm. Although vaccination side-effects are there, almost all vaccines are reportedly safe, effective and prevented serious illness, hospitalisation and death. Vaccine usually takes two weeks to manifest, and it prevented 14·4–19.8 million pandemic fatalities globally in 185 nations in a single year during December 2020 and December 2021. It was necessary that the boosters alongside vaccines enhanced immunological effects and provided extended protection. Thus, vaccine and booster design and development, dosing, targeted immunisation, cost-efficacy and vaccine acceptance are challenges to address. Government, not-for-profit organisations, health agencies and researchers may systematically analyse, justify and validate vaccine R&D and vaccinations economics. This chapter comprehends biological, commercial and economic benefits and impacts of booster dose in vaccination drives, and the roadmap ahead.

Introduction

Canine atopic dermatitis (CAD) is a chronic inflammatory skin disease marked by itching (pruritus), redness (erythema), and recurrent infections. While traditionally considered an immune-mediated condition, recent studies highlight the significant role of skin barrier dysfunction in its development. A key aspect of CAD is the compromised skin barrier, which increases permeability to allergens, irritants, and microorganisms, thereby worsening the disease. The skin barrier is a complex, dynamic structure essential for protecting dogs from environmental challenges. The stratum corneum—the outermost layer of the epidermis—serves as a crucial defense against environmental insults, allergens, and pathogens. In dogs with atopic dermatitis, this barrier often becomes impaired, leading to increased transepidermal water loss (TEWL), greater allergen penetration, and secondary infections. This dysfunction both results from and contributes to the condition, creating a self-perpetuating cycle of barrier disruption, inflammation, and immune dysregulation.

Cadmium, a roxic heavy metal, poses risk in a significant manner especially to human health and the environment. It's wide spread industrial use and presence in various products make exposure a concern particularly for both environmental and occupational settings. Understanding the source, toxico dynamics, toxico kinetics, clinical manifestations, diagnosis as well as treatment of cadmium poisoning is critical in mitigating it's adverse effects especially on human health. Cadmium gains an entry into the environment through natural processes namely volcanic activity as well as weathering of rocks. Whatever it may be, human activities along with mining, smelting, refining of metals and manufacturing of batteries, plastics as well as pigments, are major contributors to cadmium release especially into the environment. Additionally, cadmium is seen in tobacco smoke, resulting in exposure among smokers as well as passive smokers. Once released into the environment, cadmium gains entry into the human body through many routes such as inhalation, ingestion and dermal contact An inhalation of cadmium containing dust or fumes is a prominent route of exposure in an occupational settings especially in industries namely mining, smelting as well as welding. The consumption of contaminated water and food is another general route of exposure especially for the general population. After consumption, cadmium in essential manner accumulates especially in the kidneys as well as liver with a half life of approximately 10 - 30 years in the organs. It also accumulates particularly in bones, where it consists of half life of several decades.

Introduction 
The discovery of antibiotics—one of the greatest medical breakthroughs of the 20th century—began when Sir Alexander Fleming noticed that the fungus Penicillium notatum inhibited the growth of Staphylococcus spp. This finding sparked a search for similar properties in other fungi and bacteria. From 1940 to 1960, known as the ‘Golden Era of Antibiotic Discovery’, most modern antibiotics such as streptomycin, tetracycline, rifampicin, and nalidixic acid were identified through natural product screening (Clardy et al., 2006; Lewis, 2013). However, the declaration that “it is time to close the book on infectious diseases and declare the war against pestilence won” proved to be premature (Davies & Davies, 2010). It was not a final victory but a fragile truce. In the ongoing war between humans and microbes—nature’s own creations—our successes are brief, and the microbial battleground keeps shifting. Bacteria’s genetic adaptability, coupled with human complacency, led to antimicrobial resistance (AMR), where microbes gradually evolved resistance to almost all known antibiotics (Ventola, 2015). Resistance appeared soon after antibiotics entered clinical use, but the emergence of methicillin-resistant Staphylococcus aureus (MRSA), multidrug-resistant Mycobacterium tuberculosis, and extended-spectrum ß-lactamase (ESBL)-producing bacteria marked major setbacks (Prestinaci et al., 2015; WHO, 2023). AMR gained global attention with the rise of pan-drug-resistant strains—resistant to all antibiotic classes—seen in Klebsiella (India), Acinetobacter, and Pseudomonas (China, Italy, Greece), and extensively drug-resistant tuberculosis (XDR-TB). The infamous case of

The Cerebellum, a critical structure in the brain essential for coordination, balance and motor control. Can be influenced by many factors along with drugs. Adiadiochokinesia refers ro the impaired ability to perform rapid alternating movements namely rapidly pronation and supination the hands or tapping the feet. Dysfunction in Cerebellum can result in a range of motor impairment along with tremors, ataxia (lack of coordination) and dysmetria (inaccurate movements). A very few common classes of drugs associated with cerebellar dysfunction include alcohol, anti epileptic drugs, sedatives asxwell as benzodiazepines and opioid. Regarding treatment, physical therapy and rehabilitation techniques aimed at improving coordination and motor skills can be beneficial for individuals with cerebellar impairment. Finally it is concluded that drugs can exert profound effects on cerebellar function, leading to abnormalities such as adiadiochokinesia.

Introduction

The cerebellum, a critical structure in the brain essential for coordination, balance and motor control, can be influenced by various factors including drugs. One notable manifestation of cerebellar dysfunction is adiadochokinesia, a condition manifested by the inability to perform rapid alternating movements. Understanding how drugs impact cerebellar function and contribute to abnormalities like adiadochokinesia is essential for both medical professionals and the general public.

Copper is an important mineral needed fir many physiological activities especially in the human body along with the formation of red blood cells, maintenance of nerve cells and support of the immune system. Whatever it may be, like various substances copper also exhibits toxicity if present in an excessive amounts. Thus article provides an information regarding the intricacies of copper toxicity, exploring it's sources, toxico dynamics, toxico kinetics. Clinical conditions, diagnosis as well as treatment. Copper intoxication can happen through many sources such as environmental as well as occupational. Common sources are contaminated water, dietary sources, occupational exposure as well as medications. Particularly after an ingestion, an absorption of copper takes place essentially in the stomach as well as smart intestine. Now the transportation of copper happens in the blood stream, mostly in bound to ceruloplasmin, a copper _ carrying protein. An elimination of copper occurs primarily through bile secretion into the feces along with a smaller part excreted especially vin Urine. Whatever it may be, in cases of overload, an accumulation of copper takes place in tissues namely the brain, liver and kidneys, resulting in toxicity. Copper toxicity results in the formation of reactive oxygen species ( ROS ), which cause oxidative damage to cellular to cellular structures namely lipids, proteins as well as DNA. Clinical signs and symptoms include gastro intestinal portion, neurological conditions, hepatic abnormalities, hemolytic anemia as well as renal impairment. Lesions of copper toxicity are hepatic lesions, hepatic cellular necrosis, inflammation as well as fibrosis, copper accumulation, biliary changes, neurological lesions, neuronal degeneration, gliosis, de myelination, renal lesions, tubular damage, interstitial inflammation as well as vascular changes

The pandemic in 2019-2022 spread swiftly and became global in no time, caused by SARS-CoV-2. Vaccines were rapidly developed against the virus to restore public health and save global economy. Vaccines for immunisation drives were developed in a year of the pandemic becoming global and approved for ‘emergency use’ following expeditious development protocols. Vaccine development employed numerous nucleic acid, adenovirus and protein-based state-of-art molecular platforms, alongside the conventional attenuated and inactivated virus approaches. The majority of the attempts were successful and approved for use. All the approved vaccines were found safe and effective as per Phase I through Phase III clinical trials’ results. Mass immunisation drives demonstrably led to reduced hospitalisation, low disease severity and less mortality. Yet, there were initial vaccination hesitation across the world due to associated safety concerns about its possible long-term negative consequences. A part of it was attributed to technical reasons (as clinical trials were not fully complete with sturdy and convincing outcomes), and a majority was due to the misinformation that floated on the social media and other digital communications. Vaccine deployment across the globe was far from being equitable. Thus, immunisation was rapid in higher income (developed) nations with access to innovative advanced vaccines while low immunisation rates were seen in the low- and medium-income countries accessing majorly the vaccines based on simple technology. Given the scale and spread of the pandemic, scaled-up vaccine production and equitable distribution to limit viral spread and improve global health and socioeconomy were critical. This chapter attempts to dissect these scenarios and lessons learnt from these to prepare better for any such pandemic in future.

1. Introduction

India is an exquisite example of biodiversity. From time immemorial scholars like Charaka, Barathwaj, Athreyan, Agnivesha, Dhanyandhari, Shushruthan, Wakbadan and Bharathduja etc. have studied and explored the possibility of such a diversity for human welfare and the most conspicuous exploration in this field has lead to the discovery of so many indigenous medicinal plants that were scripted mainly in Vedas (1500 BC) that contain rich materials on herbal lore of that time. Charaka in his medical treatise – Charaka samhita delt with innumerable medicinal plants, their usage and descriptions. Besides the two above said texts, treatise like Sushruth samhita and Ashtangha hridaya samhita are a few among other precious ancient texts. Thus the gradual accumulation of practical and systematic medical knowledge lead to the formation of Ayurveda.

Ayurveda - the science of life, prevention and longevity, is the oldest and most holistic or comprehensive medical system available. It was placed in written form over 2000 years ago. Ayurveda is said to have been first complied as a text by Agnivesha (Agniveshtantra). This text was later revised by Charaka and renamed as Charaka samhita (completed by Dhridhabala). The concept of medicines envisaged in the Ayurveda comes from the monumental scripture called Ashtanga hridaya and Sahasrayoga.

Precision medicine and personalised treatment of systemic or tumour diseases gain their acceptance and application in veterinary care, too. The application of personalised medical protocols and companion diagnostics is easier and ethically well reasoned within the veterinary medical field – and it can offer many advantageous lessons to human clinical transformation, too [1]. In vitro companion diagnostics for testing the applicability and sensitivity levels of certain kinase inhibitor and immunotherapeutic drugs offer a greatly increased efficiency for veterinary cancer patients, too. As the understanding of cancer biology has advanced, the field of veterinary oncology has grown significantly, paralleling developments in human oncology. Precision medicine is an innovative approach to disease treatment and prevention that considers individual variability in genes, environment, and lifestyle. In the context of veterinary medicine, precision medicine aims to provide tailored therapies that are specifically designed for the unique genetic and molecular profile of each animal’s cancer. This approach moves away from the traditional “one-size-fits-all” model, instead focusing on targeted treatments that maximize efficacy while minimizing side effects. Companion diagnostics, such as molecular tests, help determine whether a dog or cat is likely to respond to a particular drug. For dogs and cats, precision medicine holds great promise in improving outcomes for cancers, which are among the leading causes of death in companion animals.

Utility of ultrasound has extended from diagnostic realm to alternate therapies. Therapeutic ultrasound is also known as ultrasound physiotherapy. It is a commonly used modality for the rehabilitation of the soft tissues of the musculoskeletal system.

ABSTRACT

Biodiversity is a major resource for drug development. Since ancient times mankind has exploited nature for all kind of useful products and enjoyed the colors, flavors and fragrances of flowers and leaves etc. The current global resurgence in the use of plant based drugs offers an ideal opportunity for the country to emerge as a major player in the herbal products market. Herbal medicines are chemically diverse and may contain thousands of bioactive compounds, which can be of immense value to the pharmaceutical industries. Since ages, herbal medicine has been a major part of traditional knowledge play an important role for identification of biological resources worthy of commercial exploitation. Origanum vulgare L. is a wild plant of Labiatae family grows widely in Ladakh region (11500 to 13000 ft). The local people of this region use this plant for various ailment and culinary purposes due to the potential of active ingredients. The present objective of this study is to isolate the thymol content, a major ingredient of O. vulgare and quantifying the same. The volatile oils were extracted through hydro-distillation and active compound was analyzed by Gas Chromatography (GC-17A Shimadzu). Our results demonstrate that the thymol content of O. vulgare from this region is 80.28% as compared to plants grown at other low altitude regions. It is well known that plants under stress respond with biosynthesis of phytochemicals to enable them to adapt to the harsh environmental conditions. Ladakh being unique in many aspects due to its geo-climatic conditions like high elevation, extreme cold, dry weather and low humidity. Plants surviving in Ladakh therefore have unique chemical composition as compared to those grown elsewhere.

Historically, the terms alternative, complementary or traditional medicine (TM) all referred to a genre of health care practices or services that got bound together as a class through the logic of reductio-ad-absurdum, or because of their “absence from the mainframe of” what has come to be known as Modern Medicine (MM). However, since Stephen Fulder¹ , the identities of TM have become more distinct, MM-TM blending more active and, the line separating the two more fuzzy. To date, the World Health Organization offers the most comprehensive definition of TM² .

WHO defines traditional medicine as including diverse health practices, approaches, knowledge and beliefs incorporating plant, animal, and/or mineral based medicines, spiritual therapies, manual techniques and exercises applied singularly or in combination to maintain well-being, as well as to treat, diagnose or prevent illness.

The phraseology MM/TM is syntactically incorrect because it is contended that medicine as such, modern or otherwise, evolves and progresses through traditions. The major difference between the various traditions of health care practices is that MM rode the crest of the high wave of reductionist-scientistic rigor of modernity³ to reach the shore of global acceptability while the others never crossed the threshold of geographical and cultural locality, remaining untested, neither proved nor disproved. Darwinian principle of survival of the fittest also endorses a truism that survival is a proof of fitness. Given this principle, it makes sense that TM as we know of it today may be useful for promoting human health. Moreover, most TM considers human beings and their health in the context of their environment. This criteria, not only defines TM apart from MM but also provides for a reason as to why lately TM is emerging out of long hibernation. Developmentally, MM isolated “human” from its global environmental context as a static, stand-alone, complete, total and delimited system in itself. While this reductionist approach has a very high pay off for the first 90% of human health problems, it fails when dynamic non-linear complex systems elements kick in (I’m not exactly sure that you mean by this phrase). TM covers some parts of those hidden variables and thats the utility to modern mankind.

TQM is an integrative philosophy of management for continuously improving the quality of products and processes. It is used around the world.

TQM functions on the premise that the quality of products and processes is the responsibility of everyone who is involved with the creation or consumption of the products or services offered by an organization. In other words, TQM capitalizes on the involvement of management, workforce, suppliers, and even customers, in order to meet or exceed customer expectations. Considering the practices of TQM as discussed in six empirical studies, Cua, McKone, and Schroeder (2001) identified the nine common TQM practices as cross-functional product design, process management, supplier quality management, customer involvement, information and feedback, committed leadership, strategic planning, cross-functional training, and employee involvement.

TQM and Six Sigma

The Six Sigma management strategy originated in 1986 from Motorola’s drive towards reducing defects by minimizing variation in processes. The main difference between TQM and Six Sigma is the approach Six Sigma is a business management strategy originally developed by Motorola, USA in 1986. As of 2010, it is widely used in many sectors of industry, although its use is not without controversy.

Q1. Alpha naphthyl thiourea is less toxic

1. In an empty stomach
2. With oily food
3. Full stomach
4. Not affected

Q2. A rodenticide that blocks tricarboxylic acid cycle by competitive inhibition of the enzyme aconitase

1. Fluoroacetamide
2. Pindone
3. Chloralose
4. All the above

Q3. ANTU is less toxic in an empty stomach a) In an empty stomach it will irritate the stomach wall resulting in vomiting and throwing away the poison and hence no toxicity.

1. Absorb only with fatty materials
2. Immediate absorption and detoxification
3. All the above
4. None of the above

Understanding the Toxicity of Macrolide Endectocides

Introduction

Macrolide endectocides are a class of compounds widely used in veterinary medicine to combat internal and external parasites in livestock and companion animals. While these medications have proven to be effective in controlling parasites, there are concerns regarding their potential toxicity and adverse effects on animals and the environment.

Whar are Macrolide Endectocides

Macrolide endectocides belong to a group of compounds derived from naturally occurring macrolide antibiotics, such as avermectins and milbemycins. These compounds have broad-spectrum activity against various parasites, including nematodes, arthropods, and ectoparasites like ticks and mites. Some commonly used macrolide endectocides include ivermectin, doramectin, moxidectin, and eprinomectin.

Toxicity Concerns

While macrolide endectocides are generally safe and effective when used according to label instructions, there are instances where toxicity can occur. Factors influencing toxicity include:

1. ABSORPTION

Deals with the translocation of the xenobiotics. Passage of toxicant from site of adminstration to blood stream or systemic circulation.

Translocation may occur by following processes

a) Simple /Passive diffusion - most common route by which xenobiotic pass through the membranes:

? Down hill transport, No energy involvement

? It is not substrate specific.

? Small molecular weight (600) hydrophilic toxicants cross membranes by simple diffusion through aqueous pores.

? High molecular wt. hydrophobic molecules diffuse across the lipid domain of membranes.

? Follows Ficks Law of diffusion

? The rate of diffusion depends on their lipid: water partition coefficient value, Conc. Gradient, degree of ionization.

? Higher lipid: water partition coefficient and lower degree of ionization favours absorption of toxicants by this process.

Introduction 
Good Laboratory Practices (GLP) serve as the foundation of any efficient and high-functioning laboratory, ensuring data reliability, patient safety and adherence to regulatory standards. In an environment where accuracy, consistency and data integrity are critical, comprehensive GLP training becomes the driving force behind operational excellence. A well-structured training program strengthens the capabilities of laboratory professionals, enabling them to navigate complex regulatory landscapes while upholding the highest standards of quality and safety. In today’s rapidly evolving scientific and industrial sectors, maintaining stringent laboratory standards is imperative rather than optional. GLP provides a structured approach that ensures every test, analysis and experimental procedure is carried out with precision, ethical rigor and strict adherence to safety measures. However, beyond simply following GLP principles, continuous assessment of laboratory personnel’s competency is essential to sustaining long-term proficiency and compliance. Competency assessment in GLP plays a crucial role in verifying whether laboratory staff possess the requisite expertise to perform tests accurately and generate reliable, scientifically valid results. As a framework designed to uphold the credibility of non-clinical laboratory studies, GLP is instrumental in reinforcing research integrity and ensuring compliance with regulatory authorities. Laboratories that rigorously follow GLP guidelines contribute to reproducible and verifiable scientific findings, which are vital for regulatory approvals and critical decision-making in pharmaceuticals, healthcare and research sectors. Competency assessments function as both a quality assurance mechanism and a professional development tool, guaranteeing that laboratory personnel not only comprehend standard protocols but can also execute them effectively in real-world scenarios. With the continuous evolution of laboratory sciences, training and assessment methodologies must also advance to meet new challenges. This chapter explores the transformation of GLP training and competency evaluation, emphasizing their significance, diverse methodologies and essential components. Additionally, it highlights best practices for implementation and introduces innovative strategies designed to complement established global standards, such as those outlined by the Clinical and Laboratory Standards Institute (CLSI) and the Indian Council of Medical Research (ICMR). By embracing these advancements, laboratories can not only achieve regulatory compliance but also cultivate a culture of excellence, ensuring that their workforce remains agile, skilled and prepared to meet the demands of modern scientific landscapes.

Introduction

Antibiotic-resistant bacteria are a major cause of health care-associated infections around the world, and resistance has also emerged in infections in the wider community. Infections caused by multi resistant organisms significantly increase morbidity, mortality, and health care costs. Molecular analyses have revealed that widespread multi resistance has commonly been achieved by the acquisition of preexisting determinants followed by amplification in response to selection. The capture, accumulation, and dissemination of resistance genes are largely due to the actions of mobile genetic elements (MGE), a term used to refer to elements that promote intracellular DNA mobility (e.g., from the chromosome to a plasmid or between plasmids) as well as those that enable intercellular DNA mobility.

Insertion sequences (IS) and transposons (Tn) are discrete DNA segments that areable to move themselves (and associated resistance genes) almost randomly to newlocations in the same or different DNA molecules within a single cell. Other elements, such as integrons (In), use site-specific recombination to move resistance genes between defined sites. As these types of MGE are often present in multiple copies indifferent locations in a genome, they can also facilitate homologous recombination(exchange of sequences between identical or related segments). Intercellular mechanisms of genetic exchange include conjugation/mobilization (mediated by plasmidsand integrative conjugative elements [ICE]), transduction (mediated by bacteriophages), and transformation (uptake of extracellular DNA). Interactions between thevarious types of MGE underpin the rapid evolution of diverse multi resistant pathogens in the face of antimicrobial chemotherapy. Primarily the most important and/ or topical elements in Gram-negative and Gram-positive bacterial species of particular concern clinically, namely, Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter spp. (the so-called ESKAPE group) (Rice LB., 2008) as well as Escherichia coli (giving the ESKAPEE group) (Llaca-Díaz J.M. et al.,2012); nonetheless, many of the MGE types described are of course broadly relevant to many other bacterial taxa.

Introduction

Mithun (Bos frontalis), or ‘Gayal’ or ‘Eso’ or ‘Sial’ or ‘Sandong’ or ‘Wei’, is a rare bovine species, exclusively found in the northeastern hill region (NEHR) of India at an altitude of 300-3000 m. It plays an important role in the socio-economic and cultural life of indigenous tribes of Arunachal Pradesh, Nagaland, Mizoram and Manipur and is aptly referred to as the ‘Pride of Northeast India’ (Mukherjee et al. 2019). Besides India, mithun population is distributed to China, Bangladesh, Thailand, Bhutan, Myanmar, and Malaysia. The Indian mithun is a medium- to large-sized majestic ruminant with a characteristic jet-black body, an ash-colored forehead, and ‘white stockings’ on all the limbs. This unique animal has a special preference for salt feeding, is a skilled browser even in steep, hilly terrain, and does not need pasture land like cattle. Mithun is primarily reared under a free range system as an important source of lean meat, rich in amino acids, proteins, PUFA, etc. On September 1, 2023, the Food Safety and Standards Authority of India (FSSAI) officially certified the mithun as a food animal (Joshi et al., 2021; Lalchamliani et al., 2024; Ramesh et al., 2024). Compared to milk from other species, mithun milk has much larger quantities of fat, lactose, solids-not fat (SNF), calcium, important vitamins A, D, and E, essential amino acids, and polyunsaturated fatty acids (Devi et al., 2023).

This chapter examines the major forms and mechanisms of immunity. It distinguishes innate immunity from adaptive immunity and explains specificity, diversity, immunological memory and self–non-self recognition. Natural and artificial forms of active and passive immunity are discussed through examples involving infection, vaccination, maternal antibodies and antiserum administration. The chapter introduces pattern-recognition receptors, pathogen-associated molecular patterns and Toll-like receptors involved in microbial recognition. It also covers inflammation, its cardinal signs, acute-phase proteins and the roles of neutrophils and other innate defence components. These objective questions provide a clear foundation for understanding how animals resist infections and develop long-lasting protective immune responses.