eChapters

Introduction

Chromatography was first employed by Ramsey (1905) to separate mixture of gases and vapours. These first experiments used selective adsorption on, or desorption from, solid absorbents such as active charcoals. The  following year Tswett (1906) obtained discrete coloured bands of plant pigments on a chromatographic column. He coined the term “chromatography” (literally “colour writing”) which is obviously a misnomer when used retrospectively. Contribution of Martin and Synge (1941) in a study for which they were awarded the Nobel prize, and later James and Martin introduced gas-liquid chromatography in 1951 and 1952.

Introduction and Scope of Biochemistry

Biochemistry is the field of study that focuses on studying chemical reactions occurring within living organisms. It is a specialized branch that combines the principles of both chemistry and biology. In order to comprehend the molecular mechanisms behind every biological event, this multidisciplinary f ield incorporates elements of biology, chemistry, and physiology. The scope of biochemistry extends from the study of small molecules and their interactions within cells to the analysis of complex metabolic pathways, enzyme kinetics, and the structure and function of biomolecules.

One of the main goals of biochemistry is to elucidate the chemical reactions that take place in living systems and understand how they regulate key biological processes. Biochemists investigate the structure, function, and properties of various biomolecules, such as proteins, carbohydrates, lipids, and nucleic acids. It helps us to study how these biomolecules interact and contribute to the overall functioning of cells, tissues, and organisms.

Biochemistry also plays a vital role in various areas of research and practical applications. For instance, in medical biochemistry, scientists study the molecular basis of diseases to develop diagnostic tests and therapeutic interventions. They investigate the biochemical mechanisms underlying genetic disorders, cancer, metabolic, and infectious diseases. Thus, understanding the biochemical basis of diseases can lead to the development of targeted and personalized treatments.

Livestock plays a pivotal role in sustaining the livelihood of millions of people worldwide, particularly in rural and semi-urban communities. Beyond providing food products such as milk, meat and eggs, livestock also contribute to income generation, draught power, manure for agriculture and socio-cultural functions in many regions. The health and productive performance of livestock animals are central to achieving the economic, nutritional and ecological goals of animal husbandry. Any compromise in animal health or productivity directly impacts farmers’ income, food security, and national agricultural output. Animal health is the foundation of productive and sustainable livestock farming. Good health ensures animals can grow, reproduce and perform at their genetic potential. Conversely, disease, poor nutrition and environmental stress can severely reduce productivity and increase mortality. Common health challenges in livestock include infectious diseases (bacterial, viral, and parasitic), metabolic disorders and management- related stress conditions. Moreover, zoonotic diseases—those transmissible to humans— are of growing concern in the context of public health and food

Introduction Identification of animals is must as a requirement in the daily management to spot and identify a particular animal in a herd/group/flock. Objectives of Identification • Identification is done to carry out routine work efficiently. • For registration and recording of the parentage in breeding programme / birth • Identification required at time of transportation and Insurance and loan from Bank. • For individual feeding of animals. • During milking • During sale, for participation of animals in the rally, show and exhibition. • For treating the animal, heat detection etc. • It is necessary for in case of theft and loss of animal for complain and search of animal. Selection of Identification Method • Depends very much on Need and personal

1. Proteins in solution absorb ultraviolet light with absorbance maxima at 280 and 200 nm. Amino acids with aromatic rings are the primary reason for the absorbance peak at 280 nm. Peptide bonds are primarily responsible for the peak at 200 nm.
2. Aromatic amino acids tyrosine and tryptophan give proteins their characteristic ultraviolet (UV) absorption at 280 nm, which is routinely used to estimate protein concentration. Phenylalanine and disulfide bonds also contribute to absorption at 280 nm, albeit slightly.

Introduction India’s animal husbandry sector plays a vital role in the country’s economy and food security. It provides livelihood to millions of people, contributes significantly to the agricultural gross domestic product (GDP), and meets the nutritional needs of the population. The sector encompasses various aspects of livestock production, including dairy farming, poultry farming, sheep rearing, goat rearing, and piggery. Milk Production India is the world’s largest milk producer, with an annual production of 230.58 million tonnes in 2022-23. This represents an impressive growth rate of 3.83% over the previous year. The top five milk-producing states are Uttar Pradesh, Rajasthan, Madhya Pradesh, Gujarat, and Andhra Pradesh. Egg Production India is also the third-largest egg producer in the world, with an annual production of 138.38 billion eggs in 2022-23. Egg production has grown by 6.77% in the last year. The top five egg-producing states are Andhra Pradesh, Tamil Nadu, Telangana, West Bengal, and Karnataka. Meat Production India’s meat production in 2022-23 was 9.77 million tonnes, with an annual growth rate of 5.13%. The top five meat-producing states are Maharashtra, Uttar Pradesh, West Bengal,

The study of general reproduction and reproductive performance in animals is a vast and intricate field that spans across various species, habitats, and biological systems. Reproduction is a fundamental biological process that ensures the continuity of life across generations. It involves a series of complex physiological and behavioural events, from mating and fertilization to gestation and birth. The general reproduction and reproductive performance in domesticated animals is a fascinating and complex field that has significant implications for various aspects of human society, including agriculture, pet ownership, and conservation efforts. Domesticated animals encompass a wide range of species, from farm animals such as cows and chickens to pets like dogs and cats, and even animals kept for conservation or recreational purposes, such as horses and certain bird species. Each of these species has unique reproductive systems and cycles, and understanding these is crucial for effective management and care. For instance, the gestation period for dogs is typically around two months, while for horses, it’s closer to a year. Similarly, some birds, like parrots, may lay eggs only once or twice a year

We may study the nutrient metabolism in a better way as explained in the following summarized form in Table 1.

Introduction

Diversity connotes multiplicity of variety. In a given ecosystem, diversity refers to presence of number of different species of ?ora and fauna that have ecological niches in agreement with the environment. Scaling up, there exists diversity between different ecosystems. All life forms of ?ora and fauna on earth contains genetic material, that is the repository of an immense amount of genetic information in the form of traits, characteristics, etc. Focusing on the species level, genetic diversity refers to genetic variation that is observed in a population. Thus, genetic diversity is the total number of genetic characteristics in the genetic makeup of a species. This provides in built latitude to the nature and/or the livestock breeders (including farmers) to manoeuvre/ manipulate so as evolve an improved population of desired qualities and traits. Further, genetic diversity is essential because the presence of adequate and appropriate genes in a population will make the species tide over adversaries owing to climate change and other emerging threats like new disease outbreaks. The ‘desired’ divergent gene pool provides the inherent resilient mechanism that equip the species to cope with likely challenging situation.

Over the years, with human interventions, the genetic diversity has been interfered with to meet specific needs; mostly with respect to garnering higher production and productivity. In the process, the diversity has been compromised for achieving the unitary goal. In certain cases, geographical barriers and other bio-physical constraints have also led to loss of diversity within a population. Studies on impact of climate change especially the effect of rise in environmental temperature on milk production of dairy animals indicate that global warming will negatively impact milk production. During 2020, the annual loss in milk production of cattle and buffaloes due to thermal stress will be around 3.2 million tonnes of milk costing more than Rs 5000 Crore at current price. Similarly, there would be prevalence of many emerging diseases including those with zoonotic implications owing to change in climatic conditions. Thus, the present day society is confronted with an imminent development dilemma of ecology vs. economics more specifically genetic diversity vs. higher production. It has led to volume of debates and discussions on the livestock development agenda to be pursued. The apparently con?icting interests has also generated antipathy amongst two schools of development.

Dairy farmers are faced with a number of fundamental inquiries. The first inquires as to what dairy animal he needs to keep. Is the breed with the best production performance the one that has adapted to the local environment the most? What animal is the best, asks the second? Is it the one with a showwinning conformation or the one with a strong ancestry? Is the ideal dairy cow one that produces the most milk, has the best feet, legs, and udder support, or combines the performance of these characteristics in the best possible way?

Genetic characterization using DNA markers is a cutting edge technology that makes it possible to study animal variability at basic level. A genetic marker can be any stable and inherited variation which is measurable or detectable by a suitable method and can be used subsequently to detect the presence of a specific genotype or phenotype other than itself, which otherwise is non-measurable of very difficult to detect. Such variations exist at morphology, chromosome, biochemistry or DNA levels that can serve as genetic markers. Molecular markers are the markers that reveal variations at the DNA sequence level. The polymorphisms observed at the DNA sequence has many applications which include percentage determination, genetic distance estimation and evolution, determination of individual identity, identification of disease carrier (heterozygous) animals, determination of twin zygosity and freemartinism, gene mapping, marker assisted selection (MAS), sex determination, DNA fingerprinting and transgenic breeding. Gene mapping is a tool to identify the genes responsible for specific traits.  The genetic map is constructed from linkage relationship based on the frequency of meiotic exchange (amount of recombination) among linked traits. Genetic map helps

Even after public concern the scientists feel that the GMO foods are quite safe. According to them the genetically-engineered crops are very safe to eat as much as the non-GE of the same crop. According to them the environment has improved by reducing the use of pesticides. GMO stands for genetically modified organism. Soy, corn, or other crops are very common as GM crops which are made by genetically engineered DNA. Many have come to the conclusion that GM foods are safe, healthy and sustainable. However, some people are against them. There are many benefits of GM crops like less water or pesticides to be used. Genetically engineered crops fight diseases to save the foods. At the same time the industries/ farmers that grow them are also benefitted. These foods have higher nutritional profiles, both in the US and abroad like corn which is one of the most prominent GMO foods. Soy is found in tofu, vegetarian products, soybean oil, soy flour and many other products. The other major crops taken for genetic modification are Papayas, Canola and Cotton. For dairy products a lot of work is being done on the genetic engineering of animals in a big way. This is going to be the most intractable environmental challenge of the 21^st Century. Presently, around 92% of the U. S. corn (GE), 94% of soybeans and 94% of cotton are genetically engineered.

GENETIC ENGINEERING

Genetic engineering is a means of improving the food supply even before harvest or slaughter by improving yields, increasing disease resistance, and enhancing the nutritional qualities of various foods. Broadly speaking, genetic engineering refers to any deliberate alteration of an organism’s DNA. Genetic engineering has been practiced for thousands of years, ever since humans began selectively breeding plants and animals to create more nutritious, better tasting foods. In the past two decades, genetic engineering has become increasingly powerful as scientific advances have enabled the direct alteration of genetic material. Genetic engineering is alternately called as ‘ recombinant DNA technology’ or ‘gene cloning’. Genes have been cut and pasted from one species to another, yielding, for example, disease- resistant squash and rice, frost-resistant potatoes and strawberries, and tomatoes that ripen-and therefore spoil-more slowly.

Genetically modified organism (GMO) is a microbe or more complex plant or animal in which DNA has been altered for a particular purpose such as manufacturing proteins, making improvement to the organism, research into the nature of genes and biological process, correcting genetic defects.

This chapter introduces genetic principles used to improve wool production and quality. It examines heritable traits including fibre diameter, clean fleece weight, staple length, staple strength, diameter variability and freedom from faults. Selection differential, response to selection, selection indices and crossbreeding strategies are discussed. The chapter also introduces objective measurement, genomic selection, reproductive technologies and electronic recording systems as modern tools for accelerating genetic improvement and producing more valuable and uniform fleeces.

Diversity of gut microbial community

The human microbiota (the collection of microbes that live on and inside us) consists of about 100 trillion microbial cells that outnumber our ‘‘human’’ cells 10 to 1, and that provide a wide range of metabolic functions that we lack. It has been estimated that they encode 100-fold more unique genes than our own genome. Furthermore, the gut microbes contribute to energy harvest from food, and changes of gut microbiome may be associated with bowel diseases or obesity. To understand and exploit the impact of the gut microbes on human health and well-being it is necessary to decipher the content, diversity and functioning of the microbial gut community. If we consider ourselves as superior organisms encompassing these microbial symbionts, by far the majority of genes in the system are microbial. In this sense, completing the human genome also requires the characterization of the microbiome (the collection of genes in the microbiota).

Methods of counting viable and non-viable bacteria in gut

Before the advent of genome sequencing and genome-wide analysis method, our knowledge of molecular characteristics of diverse gut microbial population was extremely limited. To obtain an estimate of cultivability, microbial ecologists generally compare microscopic counts with total viable counts. The total viable count made on a nonselective agar based medium estimates the number of colony forming units (cfu) per gram of sample. The finding that total viable counts are typically lower than total microscopic counts was thought to be due to the number of dead cells. Indeed, dead bacteria in feces may constitute up to one third of the total bacterial community. However, recent nucleic acid based studies indicated that a majority of bacteria in a variety of ecosystems are different from those described in culture.

Introduction

Animals have delicate balance of heat production and heat loss, which is maintained by thermoregulatory mechanisms in response to environmental temperature and humidity combinations. When the heat loss is overrun by heat gain, heat stress occurs. During heat stress, increase in the core body temperature due to failure of homeostatic mechanism reduces productivity of the animals below their original genetic potential in growth, milk production, milk constituents and reproduction (Ravagnolo and Misztal, 2002). Impending climate change scenario is a unanimously accepted reality. Increase in the anthropogenic greenhouse gases (CO₂, CH₄and NO₂) cause radiation leading to excess warming of the earth’s environmental system. According to IPCC, the predicted temperature rises for the end of 21^st century may be in the range of 1.8^oC to 4^oC (IPCC, 2007). The climate change has complex impact on the livestock production. Temperature or heat stress will be one of the major environmental factor to in?uence the health and productivity of livestock population. The least tropically adapted livestock may be severely affected by heat stress. The manage mental and ameliorative practices can deal with the heat stress in short term, however the long term focus of developing genetic heat stress tolerance in livestock population will be a significant and impactful strategy.

Introduction

The study of infectious diseases, alongside other clinical and natural strengths, is going through fast change introduced on by the approach of reasonable next generation sequencing (NGS) advancements (Koser et al., 2012).These technologies become adopted as routine methods easily, and laboratory techniques are transformed throughout the process. The amount of genomic bacterial data generated is huge. As of this writing, for example, over 200,000 Salmonella genomes alone are in the public domain with hundreds being added weekly. A whole genomic DNA sequence characterizes the maximum practicable level of structural detail on the individuating traits of an microbial organism or population. It can therefore be used to provide more accurate microbial genome recognition, evolutionary relations and a detailed catalogue of epidemiologically important characteristics. This has a significant role to play for outbreak research, diagnosis and treatment of infectious diseases, microbiology and epidemiology (Allard et al., 2019). Besides, DNA sequences are also a universal dataset which can theoretically be used to determine any biological function. This means that antimicrobial resistant (AMR) can be detected and to track the development and spread of AMR bacteria in a hospital or the community.

Introduction
Ghee is the pure clarified butter fat prepared chiefly from cream and/or desi butter (Makhan) to which no colouring matter is added.  Ghee manufacture has great significance and relevance to Indian masses and the dairy industry. Ghee is a very popular product since ancient time and has greater demand during festival and other ceremonial functions where use of ghee in food has been considered to delicacy due to its pleasing flavour and aroma. There are sufficient recorded evidence to prove that the manufacture of ghee originated from India and has been used extensively for religious and dietary purposes since Vedic times. Presently about 28% of total milk production is utilized for manufacture of about 1 million tones of ghee per annum. It is the traditional product with an established market. Cow ghee is golden yellow in colour, and is attributed unique therapeutic value.  The colour of buffalo ghee is greenish yellow due to presence of pigments called billirubin and billiverdin. Ghee is characterized by its pleasant, cooked and rich flavour. The preferred texture is of larger uniform size from grains having non-greasy consistency.

1. Key Forces Shaping Food Packaging • Changing Consumer Priorities • Modern consumers are increasingly choosing food packaging that aligns with values of health, convenience, and sustainability. The demand is growing for packaging options that are recyclable, biodegradable, or reusable. Consumers also expect transparency, with clean labels and QR codes providing details on ingredients and sourcing, which has become essential for building trust (Santeramo et al.,2022) • Environmental Sustainability • Sustainability remains a powerful influence on packaging innovation. Reducing single-use plastics, increasing recyclable options, and adopting circular economy principles are all priorities. Both consumers and businesses are actively seeking solutions that reduce environmental impact, such as renewable, compostable materials and designs that minimize waste ( Fao et al ., 2022)