eChapters

Abstract

Pomegranates are usually consumed as fresh fruit, with only a limited quantity being processed into products like juice, syrup, beverages, wine, confectionary and anardana. The special structure of fruit is one of main factors which limit its industrial processing. Knowing the importance of health benefits of pomegranate and all related aspects, the present study was carried out in order to utilize pomegranate fruit for preparation of pomegranate mouth freshener. Arils of two cultivars like Ganesh and Araktaweredried by two drying methods viz., sun drying (SD) and cabinet drying (CD).Nutritive and palatable mouth freshener was prepared from anardana of two different cultivars as Ganesh (G) and Arakta (A). Production cost of 100g pomegranate mouth freshener is about Rs.55/. It was noticed that moisture content, acidity and ascorbic acid content decreased at the end of storage.

Chemical changes occur during oven-drying because of the direct effect of high temperature, or of enzymic or bacterial changes during the early stages of drying, or of loss of volatile consitituents. Feeds containing molasses should be ground in a mortar.

Dried materials should be ground to pass 1 mm sieve. Samples expected to moisture during the treatments are usually predried. If wet samples are used directly in the assay, sufficient large quantity has to weighted out. In the first stage, roughages about 250-1000g are used, somewhat dependent upon homogeneity and moisture content of the wet material. These samples are weighted into trays and dried in draft oven at 60-70ºC. The dry samples are then again weighed, determined the moisture content. The trays are then left to equilibrate in air at room temperature for two days. before a second reweighing of the trays with samples. Then the sample is milled and filled into airtight containers and labelled as dry samples.

1. Preparation of Standard Sodium Hydroxide Solution

 Introduction

 When oxalic acid reacts sodium hydroxide, sodium oxalate and water are

form. Phenolphthalein indicator detects the end point by its characteristics

colour change.

 Apparatus

 Burettes, pipette, volumetric flask, beaker

 Reagents

 • Oxalic acid crystals

 • Sodium hydroxide – 0.1 N approximate

 • Phenolphthalein solution – 0.1 percent in ethyl alcohol

Procedure Preparation of 0.1 N oxalic acid solution 1. Weight accurately 1.575 g of oxalic acid into a 250 ml beaker using a weighing bottle. 2. Add some distilled water to the beaker and dissolve the oxalic acid with the help of glass rod. 3. Transfer the solution into a 250 ml volumetric flask through a funnel. 4. Wash the beaker 3- 4 times with small quantities of distilled water and transfer the washing into the flask. 5. Make up the volume up to the mark with distilled water and mix thoroughly. 6. Preparation of approximately 0.1 N sodium hydroxide solution 7. Weigh about 4.25 g of sodium hydroxide pellets and dissolve into 200 ml of distilled water.

9.1. INTRODUCTION

A data are generally voluminous, so they need to be put in a compact and presentable form. This chapter deals with presentation of data precisely so that the voluminous data collected could be made usable readily and are easily comprehended. There are generally three forms of presentation of data:

Textual or Descriptive presentation

Tabular presentation

Diagrammatic presentation

Bacterial species vary greatly in the ability of their cultures to remain alive after the completion of growth. Some species such as Neisseria gonorrhoeae and Streptobacillus are poorly viable and their cultures usually die out within a few day, whether kept at 37°C at room temperature or at 4-5°C; thus, they must be sub cultivated every 2-4 days for maintenance in the laboratory. Other species are much hardier,. especially sporing species which may remain viable for many years. There are also many non - sporing species (enterobacteriaceae) whose cultures, under suitable conditions, commonly remain viable for many months and often for as much as 10-20 years.

8.1 Introduction

Egg is a perishable product having short shelf life. The table eggs are of highest quality when they are laid. Preservation of eggs should start from the point of production itself.

The following practices are recommended as routine for the production of quality eggs on the farm.

1. Collection of eggs at least 3 times daily.

2. Using a clean receptacle with ventilated sides and bottom, preferably filler f lats.

Preservation of foods including meat and aquatic foods is a creation of conditions for the food articles in such a way to stop or greatly slow down the activities of micro-organisms, chemical and enzymatic reactions and physical forces to prevent the meat and aquatic foods from spoilage while maintaining its nutritional value and sensory attributes.

Significance of Meat Preservation

1. Meat and aquatic foods are perishable food items and are very prone to spoilage and rancidity development due to high nutrients, moisture and neutral pH.
2. Once meat and aquatic foods is exposed to microbial contamination, chemical and enzymatic reactions then it will be very difficult to attain asepsis.
3. To enhance the keeping quality of meat and aquatic foods so that it will be available for further processing.
4. It protects meat and aquatic foods from shrinkage, sweeting and other physical forces.
5. To protect human health by providing the wholesome meat and aquatic foods to the consumers.
6. By this way we can minimize utilization of energy in processing of meat and aquatic foods.

Preservation of raw milk is essential to maintain its quality during storage and transportation before processing. One of the primary methods for extending its storage life is refrigeration, where milk is cooled to 2°C to 4°C immediately after milking. This significantly slows down the microbial growth and enzymatic activity. Bulk milk coolers (BMCs) are vital in achieving rapid cooling, especially in collection centres or dairy farms, where they ensure that milk remains fresh during storage and transportation. BMCs are particularly essential for regions with inadequate cold chain infrastructure, as they help maintain milk quality until it reaches processing facilities.
In addition to refrigeration, thermisation is another method used to preserve raw milk. Thermisation, a mild heat treatment, is widely used to extend the shelf life of raw milk, particularly when it needs to be transported over long distances. This process involves heating the milk to 57–68°C for 15 seconds, effectively reducing the microbial load while preserving the milk’s natural characteristics. Thermisation is typically followed by refrigerated storage, where milk is cooled to temperatures between 2° and 4°C to inhibit further microbial growth. The combination of thermisation and refrigeration ensures that milk retains its freshness and safety until it reaches processing facilities, even in regions with extended supply chains.
Raw milk also benefits from its natural inhibitors, such as lactoperoxidase, lactoferrin, and immunoglobulins, which exhibit antimicrobial properties and slow down bacterial growth. The lactoperoxidase system, for instance, can be activated by adding small amounts of hydrogen peroxide and thiocyanate to enhance milk’s preservation in the absence of refrigeration. The use of chemicals like hydrogen peroxide (H2O2) for milk preservation is not permitted in India due to safety and quality concerns. However, the FAO recommends its application under strict regulatory conditions in some countries as part of the lactoperoxidase system, particularly in regions where refrigeration is unavailable.

13.1. Introduction

Diseases of dairy cattle cause huge economic losses. The losses are associated with lower productive and reproductive performances as well as mortality of the valuable animals. The high producing animals are susceptible to various infectious and contagious diseases. The diseases may permanently affect the performance of the animal e.g., mastitis may permanently damage the quarter(s) of the udder. Thus the culling rate of the herd may increase which affects the profitability of the farm. The occurrence of diseases in cattle is preventable. So, steps are needed to prevent the occurrence of diseases as prevention is better than cure. A number of factors are associated with the occurrence of diseases in animals. Disease prevention and control involves measures designed to prevent or reduce as much as possible the incidence, prevalence and consequences of the disease. These measures can be applied to control the reservoir of infection, break route of transmission or reduce the numbers of susceptible. Various new and environment friendly methods have been developed by modification or refinement of the old methods of disease control and prevention.

The purpose of PCA 1960 is to protect animals from being subjected to unnecessary pain or suffering. This at consists of 6 chapters covering 41 sections. This has been amended in 1982. The sections under each chapter have been concised for easy understanding. This provides only baseline information about the act.

Price determination under perfect competition - It can be called as a market structure characterized by a complete absence of competition or rivalry among the individual firms. The sellers and buyers cannot determine the price, only the Industry decides the price of the goods. It means the forces of supply and demand determine the price of the goods

Perfect competition is defined as a market situation where there are a large number of sellers of a homogeneous product. An individual firm supplies a very small portion of the total output and is not powerful enough to exert an influence on the market price. A single buyer, however large, is not in a position to influence the market price.

Demand under perfect competition

Demand refers to the quantity of a product that consumers are willing to purchase at a particular price, while other factors remain constant. A consumer demands more quantity at lower price and less quantity at higher price. Therefore, the demand varies at different prices. Market demand means the sum of the quantity demanded by individual buyers at different prices.

Abstract

Inspired by the human eye to brain assessment process, computer vision technology has demonstrated its potential for monitoring and controlling of several operations in food industry. Computer vision technology is, therefore, applied extensively in food drying process for monitoring nutritional value and sensorial characteristics because of its promising characteristics such as reliable objectivity and repeatability. In this chapter, after providing a brief explanation of the computer vision technology and its prerequisites and principles, an example is given to elucidate how this technology is used for monitoring the visual attributes of foodstuffs during drying process. Overall, monitoring the visual properties of food products like size, shape, color, and texture in off-line mode are the most popular issues to fascinate researchers’ attention to apply the computer vision technology in food drying. Nevertheless, industrial applications of the computer vision technology for real-time monitoring and control of food drying facilities as final goals of its introduction in food drying industry seem to be limited. For future studies, it is suggested to apply this unique technology along with advanced soft computing methodologies for in-line monitoring and control of food drying process in order to achieve high quality dried products. Finally, outlooks and potential employment of this technology in food drying domain are discussed as well.

There are potential beneficial medicinal uses of probiotics for which research is limited and only preliminary results are available. Recent research on the molecular biology and genomics of Lactobacillus has focused on the interaction with the immune system, anti-cancer potential, and potential as a biotherapeutic agent in case of antibiotic-associated diarrhoea, traveller‘s diarrhoea, pediatric diarrhoea, inflammatory bowel diseases and irritable bowel syndrome (Ljungh et al., 2009). Studies of some probiotics tested in vitro on human epithelial cell lines have shown that they could block the adhesion of shiga toxin producing E. coli (Sherman et al., 2005). These are claimed to be health promoting and/ or disease preventing beyond the basic function of supplying nutrients. Probiotic foods are emerging as an important category of food supplements. Earlier workers have shown the inhibition of diarrhea caused by E.coli using probiotic lactic acid bacteria and yeast (Yan and Polk, 2006). Ratledge et al. (2002) have studied the biochemistry and molecular biology of lipid accumulation in oleaginous microorganisms.

Abstract

Nutraceuticals have potential to be used as food supplement, preventive medicine and the growing evidence points in the direction that certain foods fight and or prevent against diseases. The development of next generation nutraceutical “super foods” or products consist of value-addition in the traditional natural diets.The word Nutraceuticals combine ‘nutrition’ and ‘pharmaceuticals’ to mean that they can be used as preventive drugs or food supplements. The increase in knowledge about the immune response unveils the fact that besides immunological disorders and infections the outcome of many other diseases like diabetes, hypercholesterolemia, rheumatoid arthritis, etc. can be modified by modulating Immune response. Hence, the efficient immunomodulators can be employed to prevent or cure the diseases as immunotherapeutic agents. The most acceptable alternative therapeutic agents are the edible sources. Hence the researchers shifted towards formation of nutraceuticals or foods acting as medicine, a concept which was given by Hippocrates long back.

Introduction

The term probiotic is derived from Greek and means ‘for life’ as opposed to antibiotics which means ‘against life’. The history of probiotics began with the consumption of fermented foods which was first observed in ancient Greeks and Romans [1]. The term, probiotic, as is used today was first used by Parker in 1974. Parker defined ‘probiotic’ as substances and organisms which contribute to intestinal microbial balance. In 1989, the term was modified further by Fuller. Thus, probiotic is a live microbial supplement which affects the host’s health positively by improving its intestinal microbial balance. This definition was broadened to include mono or mixed culture of live microorganisms which benefits animals or man by improving the properties of the indigenous microflora [2]. Some authors have interpreted probiotics as microbial cell preparations or components of microbial cells that have a beneficial effect on the health and well-being of the host.

Introduction

Let food be thy medicine and medicine be thy food- the age-old quote by Hippocrates, is certainly the tenet of today. Microbial cultures have been used for millenniums in food and alcoholic fermentations and in the past century have undergone scientific scrutiny for their ability to prevent and cure a variety of diseases. Later it became popular in improving human and farm animal nutritive values and recently in aquaculture also. The growing interest in the understanding the role of food in human health has moved from its primary role as a source of energy to the subtle action of biologically active food components on human health. Henceforth functional foods or nutraceuticals are of great demand in present time. The growing scientific evidence suggests that the food supplements containing beneficial bacteria can provide an array of benefits to the host. Now a day’s probiotic fermented foods are in great demand due to the awareness of beneficial aspects of probiotics among people. Several industries focused on the improvement of conventional fermented foods scientifically in order to fetch good market for their products. Particularly, animal feed companies and researchers have been looking for alternative products and strategies that can help to maintain animal gut health in order to prevent or reduce the prevalence of pathogens in the food chain. After understanding the mechanism and beneficial aspects of probiotics in detail at molecular level in earlier chapters, now it is relevant to discuss the applied part of probiotics. In this chapter, commercial importance of probiotics and probiotics derived products at industrial level are emphasized.

Introduction

During the first 50 years of the 20th century, scientific focus was on the identification of essential elements and their role in the prevention of various dietary deficiency diseases. This emphasis on nutrient deficiencies or “undernutrition” shifted dramatically, however, during the 1970s when diseases linked to excess and “overnutrition” became a major public health concern. “Let food be thy medicine and medicine be thy food”, the age old quote by Hippocrates is the ideology of today’s health conscious population. Eli Metchnikoff, the Russian Nobel prize winner was the first one to recognize the beneficial role of selected bacteria on gastrointestinal tract of humans. Subsequently the “Theory of Longevity” by Metchnikoff was correlated with prolonged youth and a healthy old age, observed largely in Balkan peasants of those times, who used cultured milks in their diet (Kaufmann, 2008). Since then, the quest continues for understanding the role of wide range of food components and nutrients in enhancing health or preventing chronic diseases. The research in this field has resulted in a plethora of new labels for pro-, pre- and synbiotics.

Abstract

The gut microbiota, microbe population living in the human large intestine is generally associated with various metabolic functions and it influences health and well-being. Taking into account the major role gut microbiota play in the normal functioning of the body and the different functions it accomplishes, experts nowadays consider it as an “organ”. However, it is an “acquired” organ, as babies are born sterile; that is, intestine colonisation starts right after birth and evolves as we grow. Each of us has a unique microbiota, as the fingerprints, it always fullfils the same physiological functions and they have a direct impact on our health. Gut microbiota consists of a complex of microorganism species that live in the digestive tracts of animals and is the largest reservoir of microorganisms mutual to humans. Gut microorganisms benefit the host by gleaning the energy from thefermentation of undigested carbohydrates and the subsequent absorption of short-chain fatty acids. The most important of these fatty acids are butyrates, metabolised by the colonic epithelium; propionates by the liver; and acetates by the muscle tissue. The human body carries about 100 trillion microorganisms in its intestines, a number ten times greater than the total number of human cells in the body Guarner and Malagelada, 2003). Gut microbiota, particularly the probiotics, have beneficial effects on gastroenteritis resistance, blood lipids, antitumor properties, lactose tolerance and gastrointestinal immunity. Probiotics have role in reducing the risk of rotavirus-induced diarrhea and colon cancer. The survivability of any probiotic bacteria till a longer time in the gut can be enhanced by the supplementation of prebiotics. Prebiotics are non-digestible food ingredients that benefit the host by selectively stimulating the growth or activity of one or a limited number of bacteria in the colon. There are so many prebiotics which stimulate the growth and survivability of probiotic bacteria such as fructo-oligosaccharide (FOS), inulin, galacto-oligosaccharide (GOS), polydextrose (PDX), acacia gum (GUM), lactulose (LOS), lactitol etc. Many research studies have been done on above mentioned prebiotics. Mostly inulin-type fructans have been used as functional food ingredients. The prebiotics are known for reduction of disease risk such as constipation relief, suppression of diarrhea and reduction of the risks of osteoporosis, atherosclerotic cardiovascular disease associated with dyslipidemia and insulin resistance, obesity and possibly type-2 diabetes. Probiotics are beneficial microbes that confer a realistic health benefit on the host, which in combination with prebiotics, (indigestible dietary fibre/carbohydrate), also confer a health benefit on the host via products resulting from anaerobic fermentation. The combination of probiotics and prebiotics in a synbiotic might improve the survival of the bacteria crossing the upper part of the gastrointestinal tract, thereby enhancing their effects in the large bowel and immune-modulatory ability.

The increasing use of antibiotics in livestock and poultr y led to development of antibiotic resistant micro organisms and antibiotic residues in livestock products besides other side effects. These caused alarm bells ringing all over the world and by the end of June, 1999 majority of antibiotics as growth promoters in monogastric diets had been banned within the EU. The ban on use of antibiotic at sub therapeutic level in Europe and the potential for a ban in the United States have made scientists to seriously think of alternatives to antibiotics. In fact, the use of probiotics for farm animals was already stimulated by the findings of the Schwann Committee in 1969 which recommended that antibiotics in animal feeds should be restricted. However, the concept of probiotics gained momentum in later part of the last century. In recent years, with the public disapproval of using antibiotics in livestock and poultry production due to their residual effects in their products, there has been a greater emphasis on the use of probiotics in poultry.