eChapters

(Method of Bloor, 1928 Modified by Folch et al., 1957)
Gravimetric Method — Principle

Total lipids in plasma consist of neutral fats, phospholipids, chloesterol esters and free cholesterol. Minor components include non-esterified fatty acids (NEFA), phosphatides and sphingo myelin. Total lipids in serum or plasma are extracted using ethyl alcohol and petroleum  ether, separated and weighed after drying at 105ºC.

Procedure
Pipette 5 ml serum in a separating funnel, add 5 ml ethyl alcohol and 12.5 ml pertroleum ether. Shake the contents of funnel two times for 3 min. atleast. Keep the contents of funnel as such for one hour. Transfer the contents of funnel in the moisture cup, dry and weigh. Calculate the result by difference method using the following formula.

Total Protein in Tissue Extract or Blood, Serum or Plasma
(Method of Folin-Ciocalteu, 1927 modified by Lowry et al., 1951)
Principle

A deep blue dye is formed when protein is treated with the phenol reagent of Folin ciocalteu. The extraordinary sensitivity of the method is due to the fact that two colour reactions are taking place simultaneously.
    a.    The biuret reaction of peptide bonds with copper in alkaline solution.
    b.    A reduction of phosphomolybdic acid and phosphotungstic acid by the aromatic amino acids, tyrosine and tryptophan, present in the protein.

TVFA Estimation in Blood
(Method of Friedemann, 1938 modified by Mc Anally, 1944)

A method for the determination of volatile fatty acids in various biological materials has been described by Friedemann (1938) which is said to be applicable to blood, then the blood is added to dilute sulphuric acid, then sodium tungstate solution and solid magnesium sulphate are added and the whole is steam distilled.

Reagents
    1.    Saturated magnesium sulphate solution to which has been added 2.5 per cent by volume of concentrated sulphuric acid.

Livestock poisoning by plants is often a result of management errors, range conditions, or the type of animal, rather than just the presence of poisonous plants. Forage availability can be influenced by drought, overgrazing and snow storms, or altered grazing patterns. Animals can become overly hungry during transportation or other management activities that deprive them of food and water for extended periods of time, thus making them more likely to graze toxic plants. Grazing during the wrong season may compromise an animal's ability to select adequate and non-toxic forages (James, 1994).

The toxicity of plants varies with environmental factors such as site, temperature, precipitation, and light, time of day and stage of growth. For example, mature oak leaves are less toxic than young leaves. Cyanogenic plants such as arrow grass are more toxic following a frost or drought condition. Nitrates can be higher in plants following heavy nitrogen fertilisation or during a drought. Certain animal factors must also be considered in livestock poisoning by plants. For example, goats are considered to be comparatively resistant to lantana poisoning than cattle. A hungry animal is more susceptible to poisoning than a full one. A full rumen slows consumption and also dilutes the toxin. Though the animals are exposed to a large number of toxic plants when they are left for grazing, five will be discussed in detail in this chapter. These include lantana, ageratum, eupatorium, oak and ferns (James, 1994).

Cheese, butter, whole milk powder, and skimmed milk powder are a major part of the world’s dairy trade. The demand for worldwide dairy exports is highly concentrated. With a share of 12 per cent, New Zealand is the largest exporter of dairy products, followed by Germany, Netherlands, France and the US. Germany has been the world’s major dairy importer as well with a share of 10 per cent. Other large dairy product importers are China, Netherlands, France, Italy and Belgium.

Before 1999, India was a net importer of dairy products. The successful implementation of the ‘Operation Flood’ program and trade reforms caused the direction of India’s dairy trade to change marginally. India has been a net exporter of dairy products since 2000. Statistics show that India’s export share of dairy products over world dairy exports increased marginally from 0.1 per cent in 2001 to 0.5 per cent in 2019, while its import share of dairy products over world dairy imports increased marginally from 0.018 per cent in 2001 to 0.04 per cent in 2019. The Middle East, South Asia, South East Asia, and the USA are the major markets for India’s dairy products. Conversely, India is a favorable market for the European Union, New Zealand, Australia, UAE, and US dairy products.

Export and import- an overview

The major thrust of livestock development strategy in India has been on achieving self-sufficiency in livestock products through import substitution. Several initiatives were taken to develop the Indian livestock sector in the past and India emerged as the largest milk producer in the world and it is also one of the largest producers of other livestock commodities. However, the economic policy reforms and economic liberalization triggered in 1991 have widened market opportunities for the livestock sector. The global demand for livestock products is also on the rise. Such developments offer an opportunity for India to increase its livestock exports, especially for products like bovine meat, whose domestic demand is low. With improved domestic production and marketing efficiency, better access to expanding world market, India has the potential to become more competitive and may augment port of livestock products. The export and import details reveal that there has been a steady increase in the value of imports and exports of livestock and livestock products in India.

27.1 Introduction

Eggs containing nutrients that play fundamental roles beyond basic nutrition in consumers and their promotion as functional foods should be considered. Eggs rich in protein and offer a moderate calorie source (about 150 kcal/100 g), rich in vitamins and minerals and also great culinary versatility and low economic cost, which make eggs within reach to most of the population. Eggs are also relatively rich in fat-soluble compounds and can, therefore, be a nutritious inclusion in the diet for people of all ages and at different stages of life. In particular, eggs may play a particularly useful role in the diets of those at risk of low-nutrient intakes such as the elderly, pregnant women and children.

Introduction 
Good Laboratory Practices (GLP) serve as the foundation of any efficient and high-functioning laboratory, ensuring data reliability, patient safety and adherence to regulatory standards. In an environment where accuracy, consistency and data integrity are critical, comprehensive GLP training becomes the driving force behind operational excellence. A well-structured training program strengthens the capabilities of laboratory professionals, enabling them to navigate complex regulatory landscapes while upholding the highest standards of quality and safety. In today’s rapidly evolving scientific and industrial sectors, maintaining stringent laboratory standards is imperative rather than optional. GLP provides a structured approach that ensures every test, analysis and experimental procedure is carried out with precision, ethical rigor and strict adherence to safety measures. However, beyond simply following GLP principles, continuous assessment of laboratory personnel’s competency is essential to sustaining long-term proficiency and compliance. Competency assessment in GLP plays a crucial role in verifying whether laboratory staff possess the requisite expertise to perform tests accurately and generate reliable, scientifically valid results. As a framework designed to uphold the credibility of non-clinical laboratory studies, GLP is instrumental in reinforcing research integrity and ensuring compliance with regulatory authorities. Laboratories that rigorously follow GLP guidelines contribute to reproducible and verifiable scientific findings, which are vital for regulatory approvals and critical decision-making in pharmaceuticals, healthcare and research sectors. Competency assessments function as both a quality assurance mechanism and a professional development tool, guaranteeing that laboratory personnel not only comprehend standard protocols but can also execute them effectively in real-world scenarios. With the continuous evolution of laboratory sciences, training and assessment methodologies must also advance to meet new challenges. This chapter explores the transformation of GLP training and competency evaluation, emphasizing their significance, diverse methodologies and essential components. Additionally, it highlights best practices for implementation and introduces innovative strategies designed to complement established global standards, such as those outlined by the Clinical and Laboratory Standards Institute (CLSI) and the Indian Council of Medical Research (ICMR). By embracing these advancements, laboratories can not only achieve regulatory compliance but also cultivate a culture of excellence, ensuring that their workforce remains agile, skilled and prepared to meet the demands of modern scientific landscapes.

Training is a major manpower management function. No organization can afford not to train its employees, regardless of his previous education, training, and experience. He has to be introduced into the work environment of his new employer inducted and taught to perform his assigned tasks in a new milieu. Moreover, changing technology, increasing automation in offices and factories and in almost all institutions is bringing about redesigned or altered jobs. People are constantly being “trained” and retrained by the way their superiors and other employees associate them with work. 
Training is a part of management development and also a form of organizational development. The most effective managers are those who can best develop the human resources of an organization for the purpose of achieving organizational goals and objectives. If the employees perceive training as a means to achieve their personal goals at work, they will welcome such opportunities. Some essentials about training may be borne in mind and they are:

Introduction

Antibiotic-resistant bacteria are a major cause of health care-associated infections around the world, and resistance has also emerged in infections in the wider community. Infections caused by multi resistant organisms significantly increase morbidity, mortality, and health care costs. Molecular analyses have revealed that widespread multi resistance has commonly been achieved by the acquisition of preexisting determinants followed by amplification in response to selection. The capture, accumulation, and dissemination of resistance genes are largely due to the actions of mobile genetic elements (MGE), a term used to refer to elements that promote intracellular DNA mobility (e.g., from the chromosome to a plasmid or between plasmids) as well as those that enable intercellular DNA mobility.

Insertion sequences (IS) and transposons (Tn) are discrete DNA segments that areable to move themselves (and associated resistance genes) almost randomly to newlocations in the same or different DNA molecules within a single cell. Other elements, such as integrons (In), use site-specific recombination to move resistance genes between defined sites. As these types of MGE are often present in multiple copies indifferent locations in a genome, they can also facilitate homologous recombination(exchange of sequences between identical or related segments). Intercellular mechanisms of genetic exchange include conjugation/mobilization (mediated by plasmidsand integrative conjugative elements [ICE]), transduction (mediated by bacteriophages), and transformation (uptake of extracellular DNA). Interactions between thevarious types of MGE underpin the rapid evolution of diverse multi resistant pathogens in the face of antimicrobial chemotherapy. Primarily the most important and/ or topical elements in Gram-negative and Gram-positive bacterial species of particular concern clinically, namely, Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter spp. (the so-called ESKAPE group) (Rice LB., 2008) as well as Escherichia coli (giving the ESKAPEE group) (Llaca-Díaz J.M. et al.,2012); nonetheless, many of the MGE types described are of course broadly relevant to many other bacterial taxa.

TRANSFER

A transfer is a change in job assignment. It may involve a promotion or demotion or no change at all in responsibility and status. It is a change in assignment in which the employee moves to another job at approximately the same level of responsibility, the same skill, the level of pay. Transfer is to be distinguished from promotion and demotion which imply an ascending or descending change respectively, in the hierarchy of positions. A transfer may be either temporary or permanent, depending upon the need, and may occur within a department, between departments and divisions, or between plants or officers within a company/organization.

Introduction: Relation between transportation and meat quality
One of the fundamental aims of animal production system is to ensure better quality meat. Apart from various animal husbandry practices that affect the meat quality, the transportation of meat animals from farm to slaughterhouse and their handling at lairage, affect the meat quality greatly. For better meat quality, the pH of the meat should fall from normal pH 7.0 - 7.2 in live animal to pH 5.5 (ultimate pH) after slaughter at the onset of rigor-mortis. The low muscle pH is required to inhibit the microbial growth and meat tenderization (cathepsins system is activated at low pH). The ultimate muscle pH is achieved through the accumulation of lactic acid which is produced as a result of anaerobic glycolysis of glycogen. After slaughter, as the oxygen supply to muscle ceases, ATP is generated through anaerobic glycolysis of glycogen and in this process lactic acid is produced. ATP is required to keep the muscle in relaxed stage. This anaerobic glycolysis carries on till the glycogen reserve is exhausted. So, higher the glycogen reserve at the time of slaughter, longer the length of time duration of anaerobic glycolysis and higher the quantity of lactic acid production and greater the reduction of muscle pH. Hence, livestock should be transported and handled before slaughter carefully because stress during transit and in lairage could severely deplete the glycogen reserve of livestock.
The following facts should be especially taken care by the farmers raising livestocks for meat
1. Housing arrangement and design
• Any flaws/deficiencies in design, layout, cubicle size and its design, drainage, water bowls, slats, air circulation, etc. should be rectified

Livestock transportation is a process of carrying animal from one place to another by different means of transportation. It is a stressful, threating and not familiar event of domestic animals. Livestock is transported for many reasons like marketing, slaughter, changing of owner, re-stocking and desirable grazing area)Animals who are transported for slaughter are stressful due to various component like loading, unloading, restraining, unfavorable weather condition, thrist, hunger. During transportation there are many rules and regulation by different countries which should be strictly implemented) Cattle and other animal species should be transported with partition. Birds and lab animals are transported in cages or any airy cartridge or basket. During transportation food and water should be withheld for 24 hours. if the transportation is more than 24 hours animal should be watered and fed in transit.

Rendering is the process of transforming deceased animals or condemned meat into valuable products, such as carcass meal. Traditionally, rendering specifically referred to the extraction of fat from animal materials through heating. However, the term has evolved to encompass the production of carcass meal, meat meal, and technical fat. While the traditional method of rendering involved boiling animal remnants to extract fat, contemporary rendering includes the heating or steaming of carcasses to produce a nearly sterilized material that retains nutrients. The animal by-products utilized in rendering consist of excess fat, hooves, and offal. In the past, tallow was employed in the manufacture of soap and candles, while other by-products like heads and hooves were repurposed for fertilizers. Meat packers quickly recognized the potential for profit in processing these by-products. Today, the rendering industry generates a variety of valuable products, including both edible and inedible oils, chemicals, meat meals, and bone meals, derived from materials such as viscera, bones, trimmings, dead stock, and feathers. A substantial portion of these by-products is recycled into useful products. In developed nations, as well as in India, integrated abattoirs and meat processing facilities manage such waste utilizing advanced equipment and sophisticated processes. Pre-rendering operations: It is essential to process raw materials from the slaughter floor swiftly and comprehensively to guarantee the highest quality of the final product. Prior to placing these materials into the cooker, they require adequate preparation. Heads, feet, condemned carcasses, and bones from the boning department are initially reduced or crushed into smaller fragments using shredders. Additionally, bones can be further reduced in size with machines referred to as “pre-breakers.” Intestines and other soft tissues, which may be
contaminated with feed or manure, must be opened, cleaned, and reduced in size before being incorporated into the cookers during the inedible rendering process.

The Hans Krebs cycle, known by different names like the citric acid or tricarboxylic acid cycle, was named after the scientist Hans Krebs. The citric acid cycle is named after the citrate product formed in the cycle. The name tricarboxylic acid is given as citrate itself, which is a tricarboxylic acid due to the presence of three carboxyl functional groups (-COOH).
The tricarboxylic acid (TCA) cycle occurs in aerobic conditions and within mitochondrial ETC, involving various sequential oxidoreductase reactions. The cycle requires Acetyl-CoA (C2 molecule) and oxaloacetate molecule (C4 molecule) to begin with the formation of a citrate molecule (C6 molecule). Acetyl-CoA is generated from the pyruvate (glycolysis), the fatty acids (lipolysis), or by oxidative deamination of specific amino acids. The complete oxidation of a glucose molecule results in the end products like energy (ATP), CO2 and H2O. A molecule of Acetyl-CoA has two carbons. It is formed from a three-carbon pyruvic acid molecule generated from the glycolysis of a sixcarbon glucose molecule. Acetyl-CoA is the substrate for this cycle and is an energy-rich molecule having a thioester bond between coenzyme A and acetic acid.
8.1 Formation of acetyl-CoA from Pyruvic Acid
Pyruvate is obtained by glycolysis or the oxidation of glucogenic amino acids. The pyruvic acid produced in the cytoplasm crosses the inner membrane of the mitochondrion to enter the mitochondrial matrix, where it undergoes oxidative decarboxylation, forming Acetyl-CoA. Acetyl-CoA carries (carrier molecule) the acetyl group to the citric acid cycle. It also links various metabolic products with the TCA cycle. Such products can be from the EMP cycle, beta-oxidation, and oxidative deamination of amino acids. Oxidative decarboxylation of pyruvic acid needs a complex enzyme system known as pyruvate dehydrogenase

True protein nitrogen is also termed a Albuminoid nitrogen in animal feeds and fodders and human foods. If material (Such as seeds, seed residue or oil cake) is rich in alkaline phosphates, 1-2 ml of 10 percent solution of potash alum is added to decompose them. If this is not done Cu3 (PO4) and free alkali may be formed and the protein-copper precipitate may partially dissolve in the alkaline liquid.

Introduction

On 26^th December 2004, the Indian coastline experienced the most devastating tsunami in recorded history. The tsunami was triggered by an earthquake of magnitude Mw 9.3 at 3.316ºN, 95.854°E off the coast of Sumatra in the Indonesian Archipelago at 06:29 hrs making it the most powerful in the world in the last 40 years. In recent years increasing the vulnerability to natural disasters of developing countries is a major reason for concern. The potential loss of human lives and property due to natural hazards is very high in the urban areas of these countries. The Information obtained from conventional tsunami hazard maps does not always provide sufficient data for users .needs. The difficulty in presenting more significant information on tsunami hazard maps in conventional paper format is due to the limited available physical space of that format. Printing more information on such paper maps tends to confuse rather than provide more needed information to users. Furthermore, these kinds of maps provide little opportunity for users to ask for additional information, to determine the relationships between different kinds of information and important quantitative analyses. Furthermore, Tsunami hazards have important temporal attributes. Presenting these attributes on the map; e.g. the sequence of volcanic eruptions in a region or the frequency of tsumani in a particular area during a particular period of time is very difficult.

The machine parts, which have relative motion, rub against each other. The rubbing surfaces are never perfectly smooth, even when they are highly polished. If they are observed under a microscope, one sees small depressions and elevations which get interlocked when the machine parts are under pressure, and give rise to a resisting force called frictional force. Due to friction, the parts get worn out and become useless. Friction produces heat, which, in this case, represents loss of work. To reduce this wear and loss of power caused by friction, certain foreign substances are introduced between the rubbing surfaces which keep them apart. These substances are called lubricants; and lubrication is the science of reducing friction by the application of a suitable substance between the rubbing surfaces of bodies having relative motion.

Once entrepreneurship is decided upon, there are three main routes an entrepreneur may follow. He can start a new livestock business, he can also purchase an existing livestock business or he can take over a family livestock business. In the first case the entrepreneur begins from scratch on all his own ideas, as there are no existing problems or concerns from a previous owner. He has the freedom to start the business in his own way. However, the risk is higher and he has to devote time, effort, and money—especially for startup expenses such as equipment, building, etc. When the entrepreneur purchases an existing business he has advantages of smaller startup costs and building on the existing goodwill or loyalty of established customers. This option is good if the entrepreneur does not have a great deal of business experience. Disadvantages might include inheriting existing problems such as poor location, stiff competition, fluctuating market, equipment problems, and poor reputation. In the third instance an entrepreneur takes over family business. In this situation, an entrepreneur has the support and training available from the family members, creating trust and togetherness that bonds the business as one entity. Maintaining separate family and business relationships is the biggest obstacle to overcome, making it difficult to get away from the business. Personality conflicts, different interests, changed values, and burnout are a few reasons that familyowned businesses don?t survive to the second generation. A person who grows up in the family business may be ready to spread his or her wings and explore new career aspirations.

Abstract

Demand for starch is increasing day by day and is met by restricted range of crops. In recent years, substantial progress has been made in obtaining starch from non-conventional sources such as sweet potato, buckwheat, sago, water chestnut, which can be used as a substitute for conventional sources. So it is necessary to search for other underutilized and nonconventional sources of starch to meet the increased demand. Typha angustifolia, an aquatic weed containing appreciable amount of starch in its rhizome and roots, can be used for starch isolation to make its use in food and non-food derivatives. In many parts of the world, starch-rich roots of Typha angustifolia are eaten raw or boiled or dried roots are pulverized into a powder, and then, used as a thickener in soups or added to cereal flours to make biscuits and bread.