eChapters

Introduction

A collaborative environment is necessary to enable a nurturing future for any business or entrepreneurship to succeed. While ventures may or may not have benefitted from collaborations, it is undoubtedly a step highly considered and regarded in any kind of field be it research, business, partnership between organizations or any international collaborations. The parties involved in these collaborations/partnerships share resources and risks as per agreed. The various kinds of models are discussed in this chapter ahead.

Venture Capital

Venture capital refers to financing that comes from companies or individuals in the business of investing in young, privately held businesses. They provide capital to young businesses in exchange for an ownership share of the business. Venture capital firms usually don’t want to participate in the initial financing of a business unless the company has management with a proven track record. Generally, they prefer to invest in companies that have received significant equity investments from the founders and are already profitable. Venture capital investors also prefer businesses that have a competitive advantage or a strong value proposition in the form of a patent, a proven demand for the product, or a very special (and protectable) idea. Startups with innovative ideas and technologies that have a great potential for wealth creation, but a high risk of failure might receive funding from venture capital. Typically, venture financing comes in the form of equity shares or a claim on equity that may arise in the future, such convertible debt, allowing the venture capital firm to acquire a portion of the company. New businesses are often the focus of venture capital, and Georges Doriot, a professor at Harvard Business School, is widely regarded as the “Father of Venture Capital.”

Introduction

After introduction  of Green Revolution and White  Revolution in our country, India had high growth in food and milk production but farmers of our country had  applying more chemical fertilizers, insecticides  pesticides, weedicides and various medicines  in injudicious way  to increase grain, vegetable, fruit, flower, milk, meat and egg production . Due to this reason soil fertilely and soil health is deteriorating continuously. The soil  of food bowl states of our country viz. Punjab, Haryana, Western Uttar Pradesh and plain area of Uttarakhand deficient in organic matter, macro and micro and trace elements. Now soil became non living due to death of earthworms and beneficial micro organism. Farmers are using mainly nitrogen, phosphorus and potash in crop production. However there are 15 essential plant nutrition’s for growing of crops. Due to more application of chemical fertilizers in crop production these days’ farmers are not properly using organic, green and compost manures in crop husbandry. Soil fertility and ground water level has been declined very fast by adopting intensive farming.

Histology

Cell: Structural and functional unit of an organism

M. Schleiden & Theodor Schwan – proposed cell theory

- All living being made up of cell

Tissue: Group of cell and their extracellular product organized to perform a particular function

M. Malpighii – Father of histology

1665 Robert Hooke had successfully invented the microscope. Because of this discovery, Robert Hooke was the first one to have a close look of a cell appears to be. His description of these cells was published in Micrographia. However, the cell walls observed by Hooke gave no indication of the nucleus and other organelles found in most living cells.

1674 Anton van Leeuwenhoek witnessed a live cell (plant) under a microscope.

1775 Antoine Lavoisier first proposed a mechanism for photosynthesis, a process wherein plants plants take in carbon dioxide and release oxygen. Lavoisier was also the first to investigate cell respiration in animals.

1777-83 Chemistry became dominated by the phlogiston theory, or the hypothetical principle of fire wherein all the combustible material was part composed. In this principle, burning (oxidation) was caused by liberating phlogiston, with ash as the dephlogistigated substance.

1836 The proponent of the cell theory in Biology, Theodore Schwann, proposed that the process of fermentation is solely limited to living yeast cells in 1836. Liebig did not agree to this and instead, he proposed another alternative theory of fermentation.

1856 Louis Pasteur opposed Liebig’s chemical theory. In his experiment, he showed that fermentation depends highly on the physiological functions that occur in bacteria and in living yeast cells. This work of Pasteur in 1856 received general recognition.

1860s The view on the chemistry of life highly different from the chemistry of nonliving things. During this period, the view is that the gelatinous and homogenous form of matter in organisms or more commonly known as the protoplasm carries out all the intracellular processes. These include respiration, biosynthesis of molecules, and the breakdown of matter.

Embry Transfer Technology (ETT)

Definition: The process of removing one or more embryos from the reproductive tract of a donor female and transferring them to one or more recipient females.

Foot-and-Mouth Disease

Loeffler and Frosch in 1897. Apthovirus of Picornaviridae. Strain O, A, C, Asia-1, SAT-1, 2, 3.

Cattle as Indicator host, Pig as amplifier, Sheep as maintenance host.

Musculature of heart in calves will have striped appearance termed as TIGER HEART.

Panting is common feature or sequel following FMD due to disturb endocrine function.

Preferred cell line for virus isolation in BHK-21.

Our general environment is loaded with tiny invisible living things that influence us consistently. Microbiology is a specialized area of science that deals with living things typicallytoo little to be seen without magnification. Suchmicroscopic life forms are collectively referred to as microorganisms. Microbial kingdom incorporates the study of bacteria, rickettsiae, viruses, fungi, algae and protozo a. Virology the study of viruses, mycology study of fungi and protozoology deals with protozoa.Credit for prologue to microbial world goes to ‘Antony van Leeuwenhoek’ and to vistas of microbial activities to ‘Louis Pasteur’ and ‘Robert Koch’. This period is reffered as the “Golden Era” of microbiology on account of the significant discoveries viz: microbial fermentations, discovery of various disease-causing agents, microbes’ cultivations, differences in metabolic activities, development of attenuated strains and their use as immunoprophylactic agents. The techniques used to study bacteria and different microorganisms embody direct microscopic examination, cultivation, biochemical tests, animal inoculation, serological reactivity and recent molecular biological techniques.Definitive evidence for the cause of a given infection is established by Koch that are still used to this day to definitively prove that a microorganism causes a particular disease. Koch’s four postulates are:

Parasite- The word parasite is derived from the Greek. Para- beside and sitos- food. Parasites are those living organism which reside on or inside the body of some other species of living organism.

Host- Those organisms which provide food shelter and conducive environment to parasite for their growth, development and reproduction. e.g. Tape worm in small intestine of man and animals.

Disease = dis + aise (see + ease) old French word

Transmission- Movement of biological agents (Virus, Bacteria, Rickettsial, Fungi & Parasites) from one place to another place via vector or vehicle/ tools

Vector- Latin word- bearer

Vector is a living organism (usually an arthropod) which transfers infective forms of a parasite from one host to the other.

Pathology is derived from Greek word (Pathos=disease + logos=study). It is study of structure and function of body in disease.

Veterinary Pathology is the study of anatomical, chemical and physiological alterations in comparison to normal due to disease in animals. Pathology has responsibility to forms a vital bridge between preclinical sciences (Anatomy, Physiology, Biochemistry) and clinical branches of medicine and surgery.

General Pathology deals with the basic alterations of tissues as a result of disease. e.g. fatty changes, thrombosis, amyloidosis, embolism, necrosis etc.

Systemic Pathology is related to the alterations in tissues/organs of a particular system. e.g. respiratory system, genital system etc.

Special Pathology is the application of the basic reactions of gene general pathology to various specific diseases.

Gross or Macroscopic Pathology is the examination of tissue without the help of a microscope.

Chemotherapy

Chemotherapy is branch of pharmacology that deals with study of chemical substance intended to kills or inhibits the growth of invading microorganism or foreign cell without harm to host cells. Paul Ehrlich coined the term chemotherapy and is known as father of chemotherapy. Gerhard Johannes Paul Domagk discovered sulphonamide (sulfonamidochrysoidine). First commercially available sulphonamide marketed in the form of Prontosil dye, for which he received the 1939 Nobel Prize in Physiology or Medicine. SelmanAbraham Waksman discover streptomycin and several other antibiotics from soil actinomycetes and actinomycetes is called treasurer house of antibiotics.

α-lactam antibiotics- includes penicillins, cephalosporins, carbapenems, monobectum and beta-lactamase inhibitors. It inhibits bacterial cell wall synthesis due to inhibition of transpeptidation reaction thus inhibits peptidoglycan synthesis. Penicillins contain 6-animopenicillanic acid (((lactam ring + thiazolidine ring) eg. natural penicillins, beta-lactamase-resistant agents, aminopenicillins, carboxypenicillins, and ureidopenicillins. Discovery of penicillin was done by Alexender fleming from penicillium mold in 1928 from Penicillium notatum. Now a day’s Penicillium chrysogenum mold is a major source of penicillin production. Benzathine penicillin G is a repository salt of penicillin. Probenicid competitively inhibit renal tubular secretion of penicillin and increase the half- life of penicillin. Procaine penicillin G should not administer by i.v. route due to adversely affect the cardiac conducting system. Beta-lactamses inhibitors are calvulenic acid (Streptomyces clavuligerus), salbactum, tazobactam. They are called suicidal /progressive inhibitors. Main adverse effect associate with penicillin is immediate type of hypersensitivity reaction due to Antigen+ IgE antibody reaction.

Veterinary physiology deals with the study of normal functions of animal systems. It includes the study of how animals work and the physical and chemical processes that occur within animals. The examples of various biological processes in animals include gas exchange, blood and circulation, osmoregulation, digestion, nervous and muscle systems, respiration, growth, reproduction, endocrine function and how animals behave and function in different environmental conditions. This also includes the possible manipulation of the reproductive processes by means of hormone manipulation techniques for more efficient livestock and poultry production.

Zoonoses

Zoonoses are those diseases and infections which are transmitted between vertebrate animals and humans under natural conditions. The emergence of a pathogen into a new host species is called disease invasion or “disease emergence”.

Anthrax is a notifiable disease and primarily occurs in herbivores but it is a type of bio-terrorism agent and called as malignant pustule, malignant edema, woolsorters disease, ragpickers disease, maladi charbon, splenic fever etc. Causative agent is Bacillus anthracis contains vegetative spore and lethal dose is 2,500 to 55,000 spores.

Brucellosis is one of the world’s most widespread zoonoses caused by the genus Brucella. In human it is called Malta fever, Undulant fever, Mediterranean fever, Rock Fever of Gibraltar and Gastric Fever while in animals it is called Bang’s disease, Enzootic abortion, Epizootic abortion, Slinking of calves, Ram epididymitis and Contagious abortion etc. it is also called occupational disease.

Leptospirosis is a disease of animal sand humanbeings caused by genus Leptospira and called as Weil’s syndrome, Canicola fever, Canefield fever, 7-day fever, Rat Catcher’s Yellows, Autumn fever, black jaundice). Fletcher’s EMJH media is used to culture the organism.

Veterinary surgery: The surgery which is practiced on animals. It deals with the treatment of deformities, injuries, or disease conditions by manipulation with the hands. The term “Surgery” is derived from a Greek word – Chirurgia (Cheir – Hand, Ergon – work).

The papyri of Egypt contained information on treatment of fractures and superficial wounds.

In Vedic era, Salihotra and Palakapya were the greatest ancient Indian veterinarians. Salihotra treated horses and Palakaya elephants.

“Susruta Samhita’ is the earliest known work dealing with the medical science especially surgery.

Hobday wrote first comprehensive text on surgical diseases of the dog and cat.

The theory and practice of asepsis was introduced by Joseph Lister.

W.S. Halsted introduced the principles of surgery and use of rubber gloves.

A five-kingdom classification system fails to classify viruses because they are not considered to be living. They neither reproduce independently nor utilize energy

General properties of virus

Viruses differ fundamentally from other classes of microorganism in that:

1.They are ultramicroscopic disease-producing entities.

2.They are simple in structure, basically composed of nucleic acid wrapped up in a protein coat.

3.The genome nucleic acid is either DNA or RNA , either single stranded or double stranded, but never both

4.Viruses have no metabolic activity of their own and lack enzyme systems and other constituents fundamental for independent growth and multiplication.

5.They do not posses ribosomes, nor transfer RNA and enzymes required for synthesis of nucleic acids and proteins.

6.Viruses are incapable of growth and division.

Vitamins are naturally occurring, vital nutrients that are needed in small amounts. They are important for bone and tissue health, wound healing, growth and development, immune system function, and other biological processes. These vital organic substances serve a variety of biochemical purposes. Vitamins, like minerals, are not biosynthesized by the body. Consequently, in order to maintain our health, we must obtain them from the food we eat or, in rare circumstances, supplements.

Vitamins in Milk

Together with water- soluble vitamins like vitamin C and B complex and provitamins, milk also contains a variety of fat- soluble vitamins like A, D, E, and K. Some of these vitamins do, however, have very low concentrations.

Vitamin A (Retinol)

Plant foods contain carotenoids, which are the precursors of vitamin A, whereas animal foods provide preformed vitamin A in the form of retinyl esters.

Introduction

The basic components of diet include carbohydrate, protein, fat, vitamins and minerals. Vitamins are the essential nutrients which are not formed in our body and required in minute amount from the diet. Vitamins are crucial for the optimal functions of several enzymes; thus important for optimal growth and development. Vitamins perform various functions that help in regulation of metabolism, prevent chronic diseases, maintain natural appetite, mental health and immunity.

In 1912, Polish biochemist Kazimierz Funk coined the term ‘vitamine’;a substance that was present in rice polishing and foods that cured polyneuritis in birds and beriberi in humans. The name is from ‘vit’ for vital nutrient and ‘amine’ for its similarity as an amine. Vital signify that these factors are indispensable for life. Earlier they are identified and thought to contain amino groups, thus the word amine was used, which was true for thiamine but later work revealed that most of the vitamins do not have amino groups. Thus the word was shortened to vitamin in English. It should be noted that the term vitamin does not denote to some othern utrients such as essential amino acids, essential fatty acids, dietary minerals or other nutrients that contribute towards good health.

Vitamins may be defined as organic compound needed in minute amount through diet to perform specific biological functions for normal growth and health of the organisms.Vitamins should have following characteristics:

Vitamins are natural and indispensable nutrients that are essential for our bodies in small quantities. Vitamins are indispensable for a multitude of biological functions, including growth, development, wound healing, maintaining healthy bones and tissues, supporting the immune system, and more. These vital organic compounds exhibit diverse biochemical functions and are essential for metabolic processes. The discovery of vitamins dates back to 1912 when Casimir Funk, a Polish American biochemist, initiated research on their sources, functions, and deficiency disorders. Because of his groundbreaking work, Funk is widely recognized as the pioneer of vitamins.

Types of vitamins

The classification of vitamins is determined by their solubility, resulting in two distinct groups: fat-soluble vitamins and water-soluble vitamins.

Fat-soluble vitamins

Fat-soluble vitamins, as their name implies, need fat for absorption and are stored in the fat cells. These vitamins include Vitamin A, D, E, and K.

Introduction

A variety of glassware, plasticware, and other apparatus are used for experimental purposes in a laboratory. They need to be washed, cleaned, dried, and sometimes sterilized before they can be used in the next set of experiments. Glassware needs special care of handling during washing, drying, sterilization, etc. because even a minor mistake in handling of glassware may result in its cracking/breakage and it may become unserviceable. All of these may result in a delay in experimentation if spare piece of the glassware is not available or it may affect serviceability of the associated apparatus.

A large number of glassware such as beaker, flask, measuring cylinder, burette, test tube, etc. are regularly used in a laboratory, which requires careful handling during the different stages of experiment as well as after completion of the experiment while preparing them for another set of experimentation.

Glass being a complex silicate, its properties depends on the type of silicate anions in the structure and the cations formed. Thermal properties of glass changes by the addition of boron oxide (B2 O3 ); hence, borosilicate glass is extensively used in the laboratories. With this brief background information, let us discuss about the necessity, procedures, and precautions during washing, cleaning, drying and sterilization of laboratory-wares. Figure 3.1 shows various advantages of using borosilicate glass in a laboratory.

Good laboratory practices require clean glassware, because an experiment may give erroneous result if dirty glassware is used at any step of the experiment. Hence, the glassware must be physically and chemically clean; and in many cases, it must be microbiologically clean or sterile. The commonly used glassware in a laboratory include test tube, measuring cylinder, pipettes, beakers, flasks, microscopic slides, etc.

The productivity of waste lands is extremely low, and the individuals who own them are impoverished and compelled to work for pay. Wastelands are therefore seen as a potent weapon for combating the problems of poverty and backwardness.
Definitions
Water and land are essential for the development of agriculture. Wasteland is eventually created when there is a decrease of vegetation cover, which causes erosion to take place. Waste lands are large areas of land that have been degraded and are rather difficult to plough.
The National Wasteland Development Board (NWDB) and the Planning Commission established a Technical Task Group. According to this group, wastelands are areas of deteriorated land that are currently underutilized due to various constraints, withthe exception of current use  (CSIR 1990). Depending on the random elements, the wastelands could be categorized under
1. I.erosion caused by water
2. Erosion caused by wind and
3. The Alkalinity and Salinity
The arid, stony, steeply sloped, and snow-covered wastelands are examples of the uncultivated wastelands.

1. Natural Mineral Water- Food Safety & Standard Regulations - 2011 and IS 13428:2005 Reaff. 2009 requirements
2. Packaged Drinking Water other than Natural Mineral Water- Food Safety & Standard Regulations - 2011 and IS 14543:2004 Reaff. 2009 requirements
3. Water for processed Food Industry- IS 4251 : 1967 Reaff. 1992 requirements
4. Drinking water- IS 10500 : 2012