eChapters

Abstract
 
Within the time scale for soil formation, humans appeared to the planet within the last 1 million years. They shifted their lifestyle from hunter-gatherer to nomadic animal husbandry and finally to cultivation of domesticated crops only within the last 10,000 yrs. Earlier, these crops were cultured in a semi-nomadic style as land was plentiful, so when soil lost its productivity, people abandoned it in favor of new-land. Gradually, as land became scarcer and population exploded, agriculture evolved to continuous cropping of the same parcel of land. This discovered the major concern for soil health. As organic matter is key to soil health, humus is the last stage of organic matter decomposition. Humus is a complex aggregate of dark brown colored amorphous substances, which have originated during the decomposition of plant and animal residues by microorganisms, under aerobic and anaerobic conditions. Humus and living microorganisms are connected by numerous ways which must be appreciated in order to understand the origin and nature of humus. Our attempt is to narrate the role of organic matter and vital role of microbes in humification process.

Abstract

Excessive use and treatment of soil with pesticides under the adage, “if little is good, a lot more will be better” has played havoc with human and other life forms and can also cause populations of beneficial soil microorganisms to decline, which indirectly affect soil fertility. Soil constitutes a major environmental sink for many pesticides. These pesticides are either taken up from soil by plants and then pass into the bodies of invertebrates, water or air, directly or indirectly and affect public health and beneficial biota of the ecosystem. Bioremediation provides very attractive, eco-friendly and economic solution to many of our hazardous pollution problems. For both economic and ecological reasons, biological degradation has become an increasingly popular alternative for treatment of hazardous wastes.

Abstract

Contamination groundwater and soil due to heavy metals (HMs) has aggravated into a threat not only for people inhabiting the polluted areas but also for the vegetation of the region. Relying on the use of obligate symbionts, arbuscular mycorrhizal fungi (AMF) in remediation of contaminated sites can be a productive approach. These fungi due to high cation exchange capacity have the ability to adsorb heavy metals (HMs) on fungal tissues; they also possess enzymes responsible for reduction of metals to less toxic forms. In areas with high toxicity of various HMs such as lead, AMF extends its extraradical mycelium (ERM) beyond the depletion zone of plant roots increasing the availability of nutrients such as potassium, phosphorus, magnesium, and sulphur resulting in increased plant growth and yield. Production of phenol and organic acids by the fungus have been reported to increase iron diffusion, that is a co-factor for antioxidant enzyme superoxide dismutase. AMF sequester HMs in fungal structures and bind them in their chitin cell walls preventing their translocation to aerial plant parts.

Abstract

Contamination of environment with heavy metals has become a serious global problem due to its threat to animal and human health. Removal of this non-biodegradable, hazardous heavy metals through bio-materials has received paramount interest and intense attention in the last few decades as bio-materials play crucial role in remediating heavy metal polluted environment through a process called “biosorption”. The term biosorption, therefore, has become a popular theme in recent years due to its relatively simple, inexpensive, eco-friendly with rapid mechanism of removing heavy metals from polluted environment. There have been burgeoning studies around the globe for alleviating the problem of heavy metal pollution by selecting and developing an effective bio-sorbent with minimum treatment process for field application. This review aims to provide vast information on types of biosorbent available, their exploitation for better efficiency and their economic attractiveness for the restoration of heavy metal polluted environment.

Abstract

Study was undertaken to investigate the toxicity of heavy metals on the structure and function of ectomycorrhizal fungi and their ability to colonize the pine seedlings. Ectomycorrhizal fungi were isolated, cultured and multiplied on MMN medium. Six heavy metals (Cd, Ni, Zn, Pb, Cu and Al) were selected and different concentrations viz; 0 ppm (control), 10 ppm, 50 ppm, 100 ppm, 200 ppm and 500 ppm were used for treating the seedlings. The study was continued for one year. Different growth parameters were measured. Factors such as soil moisture, pH and soil organic carbon on ectomycorrhizal colonization were also analyzed. The mycorrhizal colonization was more in those seedlings having lower concentration of heavy metal treatment. Statistical data analysis has shown that the heavy metals had negative correlation with mycorrhizal colonization. High concentration of metals were toxic to external mycelium, which ultimately resulted in reduction of ectomycorrhizal development. The accumulation of metals in the fungal mycelium and protecting root against metal toxicity was an important factor.

Abstract

The functioning and stability of terrestrial ecosystem are determined by plant biodiversity and species composition. So far, little attention has been paid to the effects of microbe-plant interactions, particularly the mycorrhizal symbiosis, on ecosystem variability, productivity and plant biodiversity. The most common type of mycorrhizal association is the arbuscular mycorrhizal (AM) type. The role of AM fungi in the uptake of diffusion limited nutrients like P and in conferring resistance to soil-borne-diseases is well documented.

Tools of Public Awareness

The public awareness and awakening can be created among people through various means and ways. In this age of computers and communication, science and technology, information and advertisements there are several quick and effective means of spreading news. The electronic media, the press, education institution, religious, adult education, moral education, spiritual education, religious discourse etc. all are essentially complementary to each other. The governments and the policy makers can only work forwards environmental preservation on political basis. The media, if work passively on pro environmental issues, awareness can be effectively generated among people.

16.1 What is Sustainable Development?

Sustainable development (SD) is a pattern of resource use that aims to meet human needs while preserving the environment so that these needs can be met not only in the present, but also for generations to come (sometimes taught as ELF-Environment, Local people, Future). The term was first  coined by the Brundtland Commission and defined sustainable development as “development that meets the needs of the present without compromising the ability of future generations to meet their own needs” (UN, 1987; Smith and Rees, 1998)

The acts for Environment Protection are :

        The Air (Prevention and Control of Pollution) Act
        The Water (Prevention and Control of Pollution) Act
        The Wildlife Protection Act
        Forest Conservation Act
        Environment Impact Assessment (EIA)
        Citizens Actions and Action Groups
        Public Awareness and Awakening

14.1 What is Education

Education is a systematic attempt towards human learning. All learning is subjective and self related. It is neither literacy nor information. Knowledge can never be learned. Knowledge is the fruit of experience and experience is the sensation of the individual. Individual experience is the internal function and is the function of awareness and understanding. And, one of the processes of knowledge and raising awareness is to be able to identify and clarify our values. Now values in education are essential to help each one of us directly so that we may order our relationships to the environment that lies outside us. Once we are clear about values, we shall be better able to shift and control information of the natural world, make wise choices and decisions, and be creative in our mental processes.

India is home of 16.7 % of the world population living in just 2.4 % of the worlds total land area. The decennial increase in population (1991-2001) has been 18.1 equivalents to the total population of Canada, France and Germany. The population in this century has 1.6 billion by 1960.

12.1 What is Climate Change?
 
The earth receives short-wave radiation from the sun, one-third of which is absorbed by the atmosphere, ocean, ice-land and living organisms. The energy absorbed from solar radiation is balanced, in the long-term, by the outgoing radiation from the earth and atmosphere. While short-wave radiation from the sun can easily pass through the atmosphere, the long-wave radiation emitted by the warm surface of the earth is partially absorbed by trace gases in the atmosphere called greenhouse gases’ (GHGs).

11.1 Global Warming

Definition : Global warming is defined as an increase in the earth’s temperature due to the use of fossil fuels and other industrial processes leading to a build-up of green house gases (CO2, methane, ozone, nitrous oxide, and chlorofluorocarbons) in the atmosphere.

11.2 Who Coined the Term Global Warming?

The principle of global warming was first put fourth by S. Arrhenius in his paper on “the influence of carbonic acid in the air upon the temperature of the ground” in 1869. This concept gained ground in the late 1980s and early 1990s when it was found that the earth’s temperature had warmed over the past century, because of increased carbon dioxide emission.

In November 1992 a document entitled Warning to Humanity was released, signed by 1500 scientists from around the world, including 99 Nobel laureates, and the Director General of the United Nations Educational, Scientific and Cultural Organization (UNESCO). The document stated that “human beings and the natural world are on a collision course,” which “may so alter the living world that it will be unable to sustain life in the manner that we know.”

9.1 What are Natural Disasters?

The natural disasters are earthquakes, floods, droughts, famines, heat waves, cyclones, wild fire, volcanic, aerosols (small particles), and landslides.

9.2 Disaster Management

Both physical solutions and institutional strategies should be developed to manage natural disasters. For flood disaster control the water flow should be checked. Institutional strategies do not rely on physical barriers and include flood plain management, flood and disaster warning systems, education and preparedness programmes.

To a layman, wasteland is a piece of land, which is lying uninhabited or uncultivated. These lands are also commonly perceived to produce much less than their potential. Depleted of the green cover and productivity, such lands are often in a critical condition. Broadly, wastelands may be defined as land where the production of biomass is less than its optimum ‘productivity. Wasteland also refers to lands, which are economically unproductive, ecologically unstable and subject to environmental deterioration.

7.1 What is Pollution?
 
Pollution is a contamination of the environment by man-made substances or energy that has adverse effects on living or non-living matter. In simple terms, pollution can be seen as the wrong substance in the wrong place in the wrong quantities at the wrong time. This implies that harm is caused to the environment if the same substance is present at levels too low.

Types of pollution
Pollution can be categorized according to the medium in which it occurs such as

6.1 Biodiversity

Biodiversity means biological diversity. It measures the variety of the earth’s animal, plant and microbial species; of genetic difference within species and of the ecosystems that support the species in its widest. Biodiversity is virtually synonymous with “life on earth”, this word was coined in 1985. Biodiversity is the degree of variety in nature and not nature itself.

5.1 Concept of Ecosystem

Ecosystem is the basic functional unit in ecology, since it includes both biotic and abiotic environments influencing each other for maintenance of life.

A.G. Tansky in 1935 first proposed the term ecosystem. According to him, it is defined as the system resulting from the integration of all the living and non-living factors of the environment. In a simplest form, an ecosystem can be defined as a self-sustained community of plants and animals existing in its own environment. The term ecosystem is composed of two terms, “eco” means environment and “system” means an interacting and interdependent complex. It is otherwise known as bio system.

In simple words natural resources can be defined as any part of our environment such as land, air, minerals, forest, rangeland, wild life, fish and human beings.

The sum total of all physical, chemical, biological and social factors which compose the surroundings of man is referred to as environment and each component of these surroundings constitute a “resource”.  The natural resources are land, water, air, minerals, forests, rangeland, wild life or even human beings known as natural resource.

The five basic natural resources are energy, matter, space, time and diversity.

3.1 What is environment ?

The word environment is derived from a French word “environne” means to encircle or surround. Environment is defined as the circumstances or conditions that surround an organism or group of organisms. It is also defined as the complex of social or cultural conditions that affect an individual or community. Since humans inhabit the natural world and they build social, cultural and technological world, all constitute important parts of our environment.

Abiotic Environment

The physical or abiotic environment includes all those physical and non-living chemical aspects, which exert an influence on living organisms. The abiotic components are soil, water, air and energy from various sources. These provide both habitation and raw material for the synthesis of organic food.

1.1 Man’s place in Nature

All religions of the world share the view that man is the supreme being among God’s creations. He is the only one among many creatures to enjoy nature’s beauties and bounties. Nature is given to him so as to make responsible and judicious use of it. No religion justifies the most destructive attitude the modern man has developed towards his environment. All religions urge man to treat nature as a God-given gift, stating that God is omnipresent in the manifestations of the nature. It is irreligious for man to expect miracles and exploit the environment. Man must be confronted with the reality that struggle is essential for life rather than “struggle for existence.”

1    Introduction to Patent Laws    540

2.    An Overview of the Patenting Process    541

    2.1.    Inventor’s disclosure, 542
    2.2.    Patentability search, 542
    2.3.    Patent application preparation, 542
    2.4.    Patent application amendment, 542
    2.5.    Patent application examination, 543
    2.6    Main parts of a patent, 543
    2.7.    Granting of patent, 544
    2.8.    Date and term of patent, 544
    2.9.    Provisional specification, 544
    2.10.    Complete specification, 545
    2.11.      Cost of patenting, 545

1.    Introduction    496

2.    Strain Development Technologies    496

    2.1.    Screening of natural variants of microorganisms, 496
    2.2.    Genetic manipulation of microorganisms, 498
        2.2.1.    Random mutagenesis and selection, 498
        2.2.2.    Conjugation, 504
        2.2.3.    Transduction, 504
        2.2.4.    Transposition, 506
        2.2.5.      Hybridization, 507
        2.2.6.    Rare Mating, 509
        2.2.7.    Cytoduction, 510
        2.2.8.    Protoplast fusion, 511
        2.2.9.    Transformation, 511
        2.2.10.    Gene cloning and transformation (Recombinant DNA technology), 513

1.    Introduction    468

2.    Biofuel Cell    470

    2.1.    Microbial-based Biofuel Cells, 471
        2.1.1.    Microbial bioreactors producing H2 for conventional fuel  cells, 475
        2.1.2.    Microbial fuel cells producing electrochemically active metabolites in  
                the anodic compartment, 476
        2.1.3.    Microbial fuel cells operating in the presence of artificial electron relays, 477
        2.1.4.    Microbial fuel cells producing electricity during wastewater treatment, 483
        2.1.5.    Microbial fuel cell producing  electricity from acetate or butyrate, 484

1.     Introduction    424

2.    Conventional Methods of Hydrogen Production    426

3.    Biological Methods of Hydrogen Production    429

    3.1.    Hydrogen production by biophotolysis of water, 430
        3.1.1. Photolysis by cyanobacteria (Blue green algae), 430
            3.1.1.1. Nitrogenase dependent hydrogen production, 434
            3.1.1.2.  Hydrogenase-dependent hydrogen production, 437
            3.1.1.3.  Factors affecting  hydrogen production by cyanobacteria, 440
            3.1.1.4.   Bioreactors for cyanobacterial hydrogen production, 443
            3.1.1.5. Future prospects for cyanobacterial hydrogen production, 446

1.    Introduction    392

2.    Landfill Gas    393

3.    Biogas    394

    3.1.    Benefits of biogas, 394
    3.2.    Potential of biogas, 395
    3.3.    Biogas production system, 396
        3.3.1.    Raw materials, 396
        3.3.2.    Biogas technology, 398
        3.3.3.    Process of anaerobic fermentation for methane generation, 400
        3.3.4.    Conditions for anaerobic fermentation, 404
        3.3.5.    Sludge-algae-methane system, 407
        3.3.6.    Biogas plant, 408

1.    Introduction    360
2.    Feedstocks for Biodiesel Production    361

    2.1.    Fats and oils, 362
    2.2.    Alcohols, 363
    2.3.    Catalysts and neutralizers, 367

1.    Introduction    302
2.    Raw Materials for Alcohol Production    304

    2.1.    Sugary substrates, 306
    2.2.    Starchy residues (cereal grains), 309
    2.3.    Cellulosic materials (crop residues), 314

3.    Microorganisms for Alcohol Production    316

    3.1.    Comparision of ethanol production by bacteria and yeasts, 318

1.    Introduction    272
2.    Alcohols    273

    2.1.    Methanol, 274
    2.2.    Ethanol, 277
    2.3.        Butanol, 283

3.    Biodiesel    284
4.    P-series fuels

1.    Introduction    232
2.    Photosynthesis and Biomass Yields    232
3.    Biomass Properties    233
    3.1.     Polymeric and chemical composition, 233
    3.2.     Fuels analysis, 235
    3.3.     Physical properties, 236

1.    Introduction    174
2.    The Thermodynamic and Mechanistic basis of Cellular Metabolism    174

    2.1.      Adenosine triphosphate, 175
    2.2.    Coenzyme A, 176
    2.3.    Modes of ATP synthesis, 177

3.      Components of Electron Transport Chain    181

    3.1.    Electron carriers in ETC, 182
    3.2.    Complex I - NADH dehydrogenase (NADH coenzyme Q reductase), 185
    3.3.    Complex II - Succinate - coenzyme Q reductase, 187
    3.4.    Complex III - Coenzyme Q - cytochrome c reductase, 187
    3.5.    Complex IV - Cytochrome c oxidase, 188
    3.6.    ATP synthese, 188

1.    Introduction    129
2.    Soil Microbiology    129

    2.1.    Bacteria, 130
    2.2.    Fungi, 132
    2.3.    Algae and Cyanobacteria, 132
    2.4.    Protozoa, 132
    2.5.    Viruses, 135

3.    Water Microbiology    133
4.    Microbial Associations    133

    4.1.    Neutral interactions, 133
    4.2.    Positive interactions, 133
    4.3.    Negative interactions, 133

1.    Introduction    71
2.    Microorganisms in Food Industry    72
    2.1.    Microorganisms as the agents of fermentation in  
        traditional fermented foods, 73
    2.2.    Microorganisms as the source of food, 83
    2.3.    Microorganisms as a source of food supplements, 86

3.    Microorganisms in Pharmaceutical Industry    92

    3.1.    Antimicrobial agents from microorganisms, 92
    3.2.     Microorganisms as probiotics, 97
    3.3.    Microbial enzymes in  pharmaceuticals, 98
    3.4.    Mushroom nutriceuticals, 98

1.    Introduction    3
2.    History and Scope of Microbiology    4
    2.1.    Controversy over the origin of microorganisms, 5
    2.2.    Germ theory of fermentation, 6
    2.3.    Germ theory of disease, 6
    2.4.    Development of laboratory techniques and pure cultures, 7
    2.5.    Discovery of immunization, 7
    2.6.    Discovery of protection against infection, 8
    2.7.    Discovery of chemotherapeutic agents, 8
    2.8.    Microorganisms as geochemical agents, 9