eChapters

Introduction

In a research laboratory, we frequently need weigh chemicals and prepare different solutions of varying strength, as per the requirements. Accuracy in weighing and preparation of solution, with respect to weight/volume and strength, determines the success of experiment. In turn, the accuracy depends on weighing of solid chemical and/or measuring the volume of liquid chemical/making up the volume. In this Chapter, weighing of chemicals using appropriate weighing balance, preparation of solutions of different strength, their dilution, and precautions while preparing will be discuss in brief.

Weighing of Chemical

The primary method to determine the amount of chemicals to be used is weighing the chemical by using a balance.

In general, there are two types of balances to weigh chemicals in a laboratory:

1. Standard laboratory balance

2. Analytical balance

Standard Laboratory Balance

Generally, a standard laboratory balance is used for weighing a chemical when an error of ±0.02 g is acceptable. It is also used for pre-weighing, where approximate mass needs to be determined. Precise mass of the chemical is then determined using an analytical balance. Following are the steps in using a weighing balance in a laboratory.

Introduction

To accomplish experimentations in laboratories, sometimes we need microscope to test/validate our ideas/findings. A microscope is needed to examine objects which are very small which cannot be visualised by naked eyes. However, microscopic study requires some expertise in operating the microscope, particularly the sophisticated microscopes, as well as to prepare (peeling, sections, cutting, fixation, staining, dehydration, mounting, etc.) the sample, so that it can be appropriately examined for the anticipated details.

According to the scientific requirements different types of microscopes are used in the laboratories for research purpose. In this chapter, our aim is to acquaint you with the basics of using and handling of microscopes, particularly a compound microscope that is commonly used by the graduate and post graduate students for basic research.

Microscopy is often designated as the scientific investigation that is employed to review and analyse minute structures and objects using a microscope. The term “microscope” has been derived from ancient Greek words “micros” meaning “small” and “skopeîn” meaning “to look at or see,”.

Microscope

A microscope is a laboratory instrument used to examine objects (biological specimens) which are too small to be seen by the naked eye. The microscope must accomplish three tasks:

• Magnification- to provide a magnified image of the specimen.

• Resolution- to separate the details in the image.

• Contrast- to render the details clearly visible to the eyes.

Although we will tnroduce to you the different types of microscopes used for different speciments, our main focus is to discuss about compound microscope, which is commonly used in the laboratories. Compound microscope was developed around the beginning of 17th century.

Since mouth pipetting of liquid reagents in a laboratory is not advisable, different tools are needed for safe, easier, and precise dispensing of liquid reagents. This chapter is devoted to make you aware of the tools like vacupette and micropipette which can be used for the handling of liquid reagents in a safe and precise way.

Vacupette

Glass pipettes have been traditionally used by sucking liquid solvent/solution directly using mouth. For safety reasons, mouth pipetting is no longer permitted in a laboratory. Hence, to fill a pipette vacupette is used by placing it on the top of glass or plastic pipette. It acts as a device to create vacuum for filling reagent in the pipette as well as for releasing the reagent with mechanical regulation on the flow out of the liquid. Vacupette can be of different types.

1. Rubber Bulb Vacupette

Rubber bulb vacupette is often used to transfer (pump up and discharge) liquid using a pipette when a larger quantity of chemicals is to be transferred. Small/tiny rubber bulb is attached to glass/plastic pipette for drawing little amount of reagents. Small vacupette is used to precisely discharge smaller volume/ drops.

Handling of Vacupette

• Insert the top of a pipette in the bottom of the rubber bulb vacupette (Figure 6.1).

• Remove air from the pipette by squeezing valve “A” at the top of the vacupette while simultaneously squeezing the rubber bulb. The amount of air released depends on the size of the rubber bulb.

Different basic as well as advanced laboratory equipment need to be used in a laboratory while conducting experiments. Many of them like pH meter, laminar air-flow hood, viscometer, thermometer, magnetic stirrer, microwave ovens, incubators, sand-bath, water-bath, oil-bath, etc. are the basic and simple equipment which require to be used routinely while conducting experiments in a laboratory. However, basic knowledge of their operation, handling procedure, when to be used, how to be used, and more importantly what not to be done with it, etc. are essential for keeping them in serviceable condition as well as for the accuracy/precision in their operation. In this chapter, we will discuss about some of the routinely used laboratory equipment along, while some other sophisticated equipment will be discussed in other chapters.

pH Meter

A pH meter measures exactly the H+ concentration of a solution; thus indicates acidity or alkalinity of a solution with greater precision. Actually, it measures the difference in electrical potential between a pH electrode and a reference electrode, and display the results converted into the corresponding pH value. Electrode is the key part (rod-like structure, usually made of glass) of a pH meter, having a bulb at the bottom containing a sensor. The pH meters range from simple and inexpensive pen-like devices (portable/pocket pH meter) to complex and expensive laboratory instruments with computer interface (Figure 5.1).

Frequent calibration of a pH meter using the solutions of known pH (standard solution of pH 4.01, 7.00, 10.01, Figure 5.2), generally before each use, ensures the accuracy of the instrument. For very precise work the pH meter should be calibrated before each measurement. For normal use calibration should be performed at the beginning of each day. This is necessary because the glass electrode does not give a reproducible measurement over a longer period of time.

A variety of glassware like burettes, pipettes, measuring cylinders, flasks, separating funnel etc. are used for experimental purposes in a laboratory. All of these need to be used and handled correctly, otherwise the chances of experimental error and damage to the glassware increase significantly. Being made of glass, they need special care while washing and using in experiments because even a minor mistake in handling of glassware may result in breakage of the apparatus. With this precautionary note, let us discuss about some of the specific glassware used in the laboratories.

Burette

Burette is a long graduated glass tube with a stopcock at one end for delivering a known/desired volume of a liquid, especially in titration experiments. It contains a stopcock at the lower end and a tapered capillary tube as the stopcock’s outlet. The flow rate of liquid from the tub stopcock’s outlet can be controlled with the help of stopcock valve. Burette is used for measuring the volume of a solution/chemical added in a quantitative (titrimetric) estimation experiment.

Procedure of using a Burette

The burette reading must be noted before and after delivering the chemical/ solution from the burette. The difference in these two readings is the volume of the chemical/solution released out of the burette. The solution should be released slowly/drop-by-drop to avoid any experimental error/mistake. If the solution/chemical is allowed to pour out too fast, the wall of the burette will not drain properly and some liquid may remain sticking to the surface of the walls. Moreover, excessive amount of chemical may be released/dropped during the experiment, particularly during the titration experiment wherein we need to know the exact amount of acid/base needed to get neutralization point. Thus, any minor mistake may lead to get a faulty reading. The measuring capacity of a burette usually used in a laboratory is 50 mL.

Introduction

A variety of glassware, plasticware, and other apparatus are used for experimental purposes in a laboratory. They need to be washed, cleaned, dried, and sometimes sterilized before they can be used in the next set of experiments. Glassware needs special care of handling during washing, drying, sterilization, etc. because even a minor mistake in handling of glassware may result in its cracking/breakage and it may become unserviceable. All of these may result in a delay in experimentation if spare piece of the glassware is not available or it may affect serviceability of the associated apparatus.

A large number of glassware such as beaker, flask, measuring cylinder, burette, test tube, etc. are regularly used in a laboratory, which requires careful handling during the different stages of experiment as well as after completion of the experiment while preparing them for another set of experimentation.

Glass being a complex silicate, its properties depends on the type of silicate anions in the structure and the cations formed. Thermal properties of glass changes by the addition of boron oxide (B2 O3 ); hence, borosilicate glass is extensively used in the laboratories. With this brief background information, let us discuss about the necessity, procedures, and precautions during washing, cleaning, drying and sterilization of laboratory-wares. Figure 3.1 shows various advantages of using borosilicate glass in a laboratory.

Good laboratory practices require clean glassware, because an experiment may give erroneous result if dirty glassware is used at any step of the experiment. Hence, the glassware must be physically and chemically clean; and in many cases, it must be microbiologically clean or sterile. The commonly used glassware in a laboratory include test tube, measuring cylinder, pipettes, beakers, flasks, microscopic slides, etc.

Introduction

While experimenting with chemicals, sometimes we need to dry the chemical/ solution (solid, liquid or gas) using a process like evaporation or distillation, mainly to remove water or different solvents. Drying is the process of evaporation or distillation to remove water/solvent from a solution, solid or even from gas. Generally, a drying agent (usually an anhydrous inorganic salt) is used for this process, which reacts with the water (present in the chemical/ solvent to be dried) to form a hydrate. For example, anhydrous MgSO4 is one of the drying agents. The drying agents are recognized by their capacity (the rate at which they absorb water) and their intensity (the amount of water left behind in the solvent at equilibrium). No chemical reaction or change in phase occurs during the drying process.

Type of Solvents

Apart from water (a universal solvent), there are three families of organic solvents used in laboratories.

1) Oxygenated solvents: Alcohols, Ketones, Glycol ethers, Glycol esters, etc.

2) Hydrocarbon solvents: Aliphatic and Aromatic hydrocarbons.

3) Halogenated solvents: Chlorinated hydrocarbons.

Introduction

For proper and efficient experiment to be conducted, experimenters need so many chemicals to deal with. This includes different acids, base, salt, different kind of indicators and so on. Some of these chemicals are safe for health, but some of them are dangerous to health when come in close proximity to our body while some of the chemicals cause health issues on inhaling. These may cause improper functioning of some of our physiological activities. Acids and bases are very dangerous for our tissues when come in our contact. These may cause serious respiratory problem in addition to injuries on coming to our contact. Some chemicals are exploratory yet they may cause even death of the person working with them in laboratory. Therefore, mere use of the chemicals in laboratory is not only the objective of the experimenter. The person working in laboratory should also be concerned about the proper use and handing of the chemical while working with it. Thus, every researcher of an institution who has to work with chemicals in a laboratory should be made aware about the chemicals to be used in the laboratory and the consequences if any mishandling of a laboratory chemical occurs. Not only these, suitable precautionary measures should also be known to the researcher along with ensuring the availability of the required safety equipment/installations. Hence, a proper understanding of the ways of handling of chemicals in a laboratory is of prime importance for the personals like you who are working or have to work in laboratory.

Safe Handling of Chemicals in Laboratory

Safe use/handling of chemicals in laboratory include minimized exposure to chemicals, maintaining safety data sheets, proper labelling, storage, segregation, and transport of chemicals for personal hygiene.

Z -distribution: It is a probability density function and a normal distribution that has a mean equal to zero and a standard deviation equal to one and that is used in testing hypotheses about means or proportions of samples drawn from populations whose population standard deviations are known.

Zeatin: Zeatin is a cytokinin C10 H13 N5 O first isolated from the endosperm of Indian corn.

Zeaxanthin: Zeaxanthin is an isomer of lutein occurring in fruits and vegetables (spinach and corn) (Plate 343, 344 and 345).

Zein: Zein is a protein from Indian corn that lacks lysine and tryptophan and is used in making textile fibers, plastics, printing inks, edible coatings, and adhesives and sizes.

Yabby: It is one of the burrowing Australian crayfishes (Cherax destructor) that are used for food.

Yakitori: A bite-size marinated pieces of beef, seafood, or chicken on skewers is called yakitori.

Yam: It is edible starchy tuberous root of plants (genus Dioscorea of the family Dioscoreaceae) used as a staple food in tropical areas.

Yard–long bean: It is the edible 1 to 3 foot (0.3 to 0.9 meter) long thin stringless pod of a south Asian plant of a subspecies (Vigna unguiculata sesquipedalis) of the cowpea.

Yautia: It is one of the aroid plants (Xanthosomasagittifolium) chiefly of tropical America with starchy edible shaggy brown tubers that are cooked and eaten like yams or potatoes.

Yeanling: A young lamb or kid used for food is called yeanling. It is also called earling.

Yearling mutton: Meat obtained from 12-20 month aged sheep. It is also called Earling mutton..

Xanthan gum: It is a polysaccharide that is produced by fermentation of carbohydrates by a gram-negative bacterium (Xanthomonas campestris) and is a thickening and suspending agent used in pharmaceuticals and prepared foods. It is also called xanthan. Xanthan is a stabilizer produced by Xanthomonas compestris (Plate 340). Xanthan is not hydrolysed or degraded by humans.

Xanthine: A basic compound C5 H4 N4 O2 that occurs in animal or plant tissue, is derived from guanine and hypoxanthine, and yields uric acid on oxidation.

Xanthones: It is a group of yellow pigments e.g. mangiferin in mango,

Xanthophyll: Yellow hydroxyl carotene derivative (C40H550C) which is present in all green leaves together with chlorophyll and carotene. Xanthophyll is a pigment containing O2 derived from carotene.

Xeroderma pigmentosum: It is a genetic disorder inherited as a recessive autosomal trait that is caused by a defect in mechanisms that repair DNA mutations (as those caused by ultraviolet light) and is characterized by the development of pigment abnormalities and multiple skin cancers in areas exposed to the sun.

Wafer: A thin crisp cake, candy, cracker or chips is known as wafer.

Waffle: A crisp cake of batter baked in a waffle iron is called waffle

Warner-Bartzler Shear: It is one of the oldest but commonly used devices used to measure the tenderness of meat. It is used to test the quality in the cooked meat (Plate 331).

Wastage: Wastage is a term that can not be precisely defined by even moral value / judgment and defined on the context / situation on which it is used.

Waste water: Water that has been used in a manufacturing process and goes waste is called waste water. Wastewater i.e. effluent generated from various points at the plant such as outlet of Westfeling separator and Treatment tanks etc. is polluted and it must be given a treatment before draining so that pollution control board norms are met. It is achieved through a specific treatment in a plant called Effluent Treatment Plant. ETP treats the waste/polluted water coming from the plant and make it able to dispose off in the river by neutralizing its toxicity.

Water: Water (H2 O) is a clear transparent, colourless, odourless and tasteless liquid; neutral in reaction; boils at 100oC, freezes at 0oC and has maximum density (1 g/cm3) at 4oC. It exists as snow, ponds, lake, rivers, seas, oceans etc., also occurs as rain and is there in the form of moisture in atmosphere. Pure water is bad conductor of heat and electricity. Water is an essential part of the body structure as a carrier of nutrients and regulator of a number of body functions. Fruit and vegetables contain > 80% water and sometime upto 95% water e.g. cucumber, lettuce, marrow, lettuce, melon. The starchy tubers and seeds, for example yam, cassava and cork, contain less water, but even > 50%. So water content must be optimum at the harvesting time. The human body comprises of about 65 % water, while that of fruits and vegetables 80-90% water.

Vacreation: The pasteurization of the milk or cream under reduced pressure by direct steam is called vacreation.

Vacuum: The state of negative pressure.It is equal to atmospheric pressure minus absolute pressure.

Vacuum cooling: A technique of cooling vegetable (leafy vegetables, asparagus Brussels sprouts) having a high surface to volume ratio rapidly and uniformly by boiling off some of their water at 10C and at low pressure (5 mm of Hg) into a sealed container. The produce is cooled by evaporation of water from the tissue surface and is more rapid than hydro cooling.

Vacuum drier: A drier used to dry heat sensitive products where low pressure lowers the temperature, helps to retain natural flavour and minimizes oxidation and browning is called vacuum drier (Plate 325).

Vacuum gauge: A gauge indicating degree of rarefaction (state or region of minimum pressure in a medium) below atmospheric pressure.

Vacuum packed: A packhaving much of the air removed before being hermetically sealed is called vacuum pack (Plate 326).

U value: U value is a measure of the heat transmission through a building part (wall or window) or through a given thickness of a material (insulation). U values with lower numbers indicate better insulating properties.

Ubiquinone: It is one of the groups of lipid soluble quinones that are found especially in mitochondria and have a long isoprenoid side chain, and function in oxidative phosphorylation as electron carrying coenzymes in electron transport.

UHT milk: This milk is homogenized, heated to atleast 135oC for about 2-3 second, cooled rapidly and then packed in sterilized containers aseptically. The milk in packs can be stored for several months with out refrigeration. The nutrient loss is negligible. Used for making yogurt commercially as it does not need to be boiled again.

UHT pasteurization: The milk is heated to ~150oC for a fraction of seconds practically no holding period. It is also called ultra pasteurization.

UHT sterilization: It is method in which food is sterilized, cooled and then aseptically placed in previously sterilized containers which are subsequently sealed in an aseptic environment.

Ulcer: A break in skin or mucous membrane with loss of surface tissue, disintegration and necrosis of epithelial tissue (often with pus). It is something that festers and corrupts like an open sore.

Ulcerate: A tissue or skin thatbecomes affected with an ulcer is called ulcerate.

Ulceration: The process of becoming a tissue or skin ulcerated is called ulceration.

T A Spoilage: The spoilage of low and medium acid canned foods by Clostridium thermosacharolyticum which breaks sugars and form acid and gas (moisture of H2 and CO2 ) resulted in swelling and in severe cases even bursting of can may occur and the spoiled food usually has a sour odour.

T cell: It is one of the lymphocytes (helper T cell) that differentiate in the thymus, possess highly specific cell-surface antigen receptors, and include some that control the initiation or suppression of cell-mediated and humoral immunity (regulation of T and B cell maturation and proliferation) and others that lyses antigen-bearing cells. It is also called T lymphocyte.

Table cream: It is also called coffee cream, single cream or homogenized cream. It is used in coffee and tests about 20% butter fat.

Table oil expeller: It is a type of the machine that is used for extraction of the oil from the oil seeds (Plate 313).

Table ready meat: Cooked and smoked sausages are called table ready meats.

Table wine: Table wine is a dry wine with low alcohol content. Dessert wines certified sweet wine.

Saccharides: A monosaccharide sugar or combination of sugars is called saccharides.

Saccharification: The process of breaking a complex carbohydrate (starch or cellulose) into its monosaccharide components is called saccharification.

Saccharimeter: A device (polarimeter) used for measuring the amount of sugar in a solution is called saccharimeter. It is also known as saccharometer. It is a hydrometer with a special scale.

Saccharin: A crystalline compound C7 H5 NO3 S that is unrelated to the carbohydrates, is several hundred times sweeter than sucrose, and is used as a calorie free sweetener.

Saccharomyces: It is one of the genus (Saccharomyces) of the family Saccharomycetaceae and is usually unicellular yeasts (brewer’s yeast) that are distinguished by their sparse or absent mycelium and reproduced asexually by budding.

Safe food: Food that provide maximum nutrition and quality while revealing a minimal hazard to public health and they assume any risk present to be minimal.

Safety: Safety is a component of quality. Many experts believe that food safety is the most important quality component since a lack of safety can result in serious injury and even death of the consumer.

R - Enamel can: The acid resistant, gold coloured enamel treated can are called R enamel cans. It is used for canning of acidic food which have soluble colouring pigments.

R factor: It is a group of genes present in some bacteria that provide a basis for resistance to antibiotics and can be transferred from cell to cell by conjugation.

R value: R value is a measure of resistance to the flow of heat through a given thickness of a material (insulation) with higher numbers indicating better insulating properties.

Rabbitto and putridity: The surface taint of the milk caused by Pseudomonas putrefaciens is called rabbitto and putridity.

Racking: An important operation for the wine production which wine is siphoned off or bottom of the vat) and is transferred to a fresh vat with the help of a false bottom. Racking is repeated decanting to remove the slowly setting cloud of the freshly fermented young wine. It is also called clarification. It is synonym to siphoning.

Rad: It is the unit of radiation dose being equivalent to the absorption of 100 erg/g of irradiated material.

Q fever: Q fever is caused by a rickettsia like Organism (RLO), a parasitic organism falling between bacteria and viruses. It is a disease that is characterized by high fever, chills, muscular pains, headache, and sometimes pneumonia, that is caused by a rickettsia (Coxiella burnetii) of which domestic animals serve as reservoirs, and that is transmitted to humans especially by inhalation of infective airborne bacteria as in contaminated dust.

Q value: It is the quantity of energy released in a nuclear reaction expressed in million electron volts (MeV).

Q10 value: The number of times a reaction rate change with a 10oC change in temperature is called Q10 value. If reaction rate doubles with 10oC change in temperature, Q10 = 2. It is also called temperature quotient.

Q-enzyme: It is a branching enzyme responsible for formation of 1-6 bonds in amylopectin.

QHV process: It stands for Quality Has Value Process.

Pack inspection: It is similar to continuous inspection except that here inspector(s) may make in-process checks during different processing operations under contract but not required to be present all the times in the plant during operation.

Package: The package means a pre packed box, tin, barrel, pouch or can etc in which food article is packed.

Package store: A store that sells bottled or canned alcoholic beverages for consumption off the premises.

Packaging: Packaging is an industrial and marketing technique for containing, protecting, identifying and facilitating the sale and distribution of agricultural, industrial and consumer products. Packaging is defined as an enclosure of products, items or package in a wrapped pouch, bags, box, cup, bottle or any container form to perform one or more of the functions as containment, protection and preservation, communication and utility or performance. Technically, packaging is a system or method by which a product from the manufacturing point to the consumer level reaches in a safe and sound condition at an affordable cost. The primary purpose of the packaging is to protect the food product, to keep it in good condition and to preserve the flavour until it reaches the consumer. It is therefore, very essential that a suitable form of the packaging is chosen for a finished product. Packaging is an art, which determines the buying decision of a consumer. or Wrapping / placement of objects in paper / other packaging materials keeping in box, etc is known as packaging which has two main functions, to assemble the produces / objects in convenient units for handling and to protect them during transporting, marketing and storage which results in extended shelf life of the produce. “Packaging” play a very important role not only in ensuring safety of the product during transportation, storage and distribution and in conveying information about the pack contents to the consumers, but also ensuring desired sensory characteristics and micro-biological soundless till consumed.

Obesity: A condition characterized by the excessive accumulation and storage of fat in the body. It is a physiological disorder having high body mass index (BMI), which can be expressed as a mathematical formula in which a person’s body weight in kilogram is divided by the square of his or her height in meters.

Objective Methods of quality evaluation: These are based on recognized standard scientific tests to any sample of the product without regard to its previous history. They represent the modern idea in quality control (QC) because the human element has been excluded. This method divided into three groups like physical, chemical and microbiological. The devices are used in this evaluation.

Obligate anaerobes: These microbes do no grow inthe presence of oxygen.

Obligate parasite: Obligate parasites is host specific and grows on living host only and attach limited range of host and are very difficult or impossible to grow on artificial culture/media.

Obligate thermophile: These microorganisms grow at a temperature between 38 80oC.

Oedema / edema: It is an abnormal infiltration and excess accumulation of serous f luid in connective tissue or in a serous cavity. Oedema meanswatery swelling of plant organs or parts. It is also called dropsy.

Naïve panel: It refers to the sensory evaluation panel that has not been trained specifically regarding the product evaluation being undertaken in the study.

Nano technology: It is the art of manipulating materials on an atomic or molecular scale to build microscopic devices. The word “nano’ meaning ‘dwarf” in Greek language refers to dimension / magnitude of the material / particle in the order of 109. Nanotechnology is nothing but manipulating of particles into very small particles called nano-particles. These particles have dimensions in the scale of nano-meter and used to design / build materials and devices. Nanotechnology has been provisionally defined as relating to materials, systems and processes which operate at a scale of 100 nanometers (nm) or less. Nanotechnology is an anticipated manufacturing technology that allows through and inexpensive control of the structure of matter by working with atom. It will allow many things to be manufactured at low cost and with no pollution. The concept of nanotechnology was first given by Nobel Laureate physicist Richard P. Feynman in Southern California in 1959. Nanotechnology is sometimes termed as Molecular Manufacturing based nanotechnology “MNT”.

MAC: It stands for modified atmospheric coating.

Macaroni wheat: It is tetraploid wheat and is preferred for making macaroni, vermicelli etc.

Macaroni: Pasta made from semolina and shaped in the form of slender tubes (Plate 230).

Macaroon: A small cookie composed chiefly of egg whites, sugar, and ground almonds or coconut (Plate 231).

Mace: An aromatic spice consisting of the dried external fibrous covering of a nutmeg (Plate 232).

Mach number: It is a number representing the ratio of the speed of a body (flow velocity) to the speed of sound (sonic velocity) in a surrounding medium (as air).

Macro economics: Studies of economics in terms of whole systems esp. with reference to general levels of output and income and to the inter-relations among sectors of the economy.

L - Ascorbic acid: It is a white crystalline compound with a slightly acidic taste. It is readily soluble in water, alcohol, glycerin, propylene glycol and is insoluble in oils, fats, ether, benzene, chloroform.

Lab coat: A loose white coat with deep pockets that is worn in a laboratory or medical office.

Labeling: It means any written, printed or graphic matter that is present on the label, accompanies the food or is displayed near the food, including that for the purpose of promoting its sale or disposal. After packaging, labels are required on the finished products intended for distribution and sale. Labeling can reflect on the quality of the product. It must be clear and informative. Information includes Name of the Manufacturer, Date of Manufacture, Net Weight, Gross Weight, Best before etc. Proper storage is needed for maintenance of the quality. Storage must be done only at recommended temperature, relative humidity, light and air / oxygen and carbon dioxide conditions etc. to maintain the quality of the food product. Label may include tag, brand, and pictorial or discriptivematter, written, printed, embossed, graphic, perforated, stenciled or stamped impressions. It is attached to container, cover, lid, or crown of ant food package.

K ration: It is a lightweight packaged ration of emergency foods developed for the U.S. armed forces in World War II (Plate 215).

Kairomone: A chemical substance emitted by one species and especially an insect / plant that possess an adaptive benefit (stimulus for oviposition) to another species.

Kaiser rolls: A round crusty roll often used for sandwiches (Plate 216).

Kalamata/calamata: It is brine cured black olive grown in Greece.

Kallikrein: A hypotensive protease that liberates kinins from blood plasma proteins and is used therapeutically for vasodilation.

Kanji: Clean, black coloured, crisp and sound product of characteristic flavor prepared by full fermentation (chiefly lactic acid fermentation) of black carrot with 2.5% salt, 1% powder mustard and some amount of water.

Kaposi’s sarcoma: It is a neoplastic disease that occurs in individuals coinfected with HIV and a specific herpes virus (species Human herpesvirus 8 of the genus Rhadinovirus), that affects skin and mucous membranes, and is marked by pink to reddish-brown or bluish plaques, macules, papules, or nodules.

Jaboticaba: It is a Brazilian tree (Myrciaria cauliflora) of the myrtle family cultivated in warm regions for its edible purplish fruit.

Jack salmon /walleye: A large vigorous American freshwater food and sport fish (Stizostedion vitreum) that has large opaque eyes and is related to the perches but resembles the true pike called also walleyed pike

Jaggery: Raw brown / unrefined brown sugar in lumpmade from palm sap (Plate 209).

Jam: A preserved product prepared on boiling pectin contained fruit pulp having edible seed and skin with required amount of sugar, water and acid to a reasonably thick consistency, firm enough to hold the fruit tissues in position (Plate 210).

Jam pack: The packing of the commodity tightly or in excess is called Jam pack.

Jamaica rum: Heavy bodied rum made by slow fermentation and marked by a pungent bouquet.

Jamboline: A type of glucose found in the jamun fruit help in cure of the diabetes in the patients.

Ice Bank Cooler: Air forced cooler leads to desiccation of the produce. So use forced air high humidity cooler and these are known as Ice Bank Cooler. Ice bank Coolers are useful for strawberry.

Ice bank cooling: Storing of fruit and vegetable in storage chamber with positive ventilation and system countries where ice formation is allowed during night time (as electricity cost of night is less as compared to the day time). Melting of ice occurs during day time and with proper air circulation by means of ventilation process, approximately 0.5-10C temperature with 90% RH in the chamber is maintained. Ice Bank Cooler / Air forced cooler leads to desiccation of the produce (Plate 205). So use forced air high humidity cooler and these are known as Ice Bank Cooler. Ice bank coolers are useful for strawberry.

Ice cream cone: A thin crisp edible cone for holding ice cream and filled with ice cream.

Ice cream: Ice cream may be define as frozen dairy product made by suitable blending and processing of cream and other milk products together with sugar and flavour.

Ice point: It is the temperature of equilibrium between ice and water under normal atmospheric pressure (0oC).

Ichthylogy: It is the branch of the zoology that deals with the study of fishes (Pisces).

HACCP: The Hazard Analysis and Critical Control Points (HACCP) is a scientific, rational and systematic approach to identify, assess and control hazards during production, processing, manufacturing and use of food. It ensures safety of the food. HACCP presents a good opportunity for the food industry for upgrading quality and bringing uniformity and consistency in their supplies to international market. or It is a systematic preventive approach to food safety that addresses physical, chemical and biological hazards as a mean of prevention rather than finished product inspection. It involves identification, evaluation and control of hazards which are significant for food safety.

HACCP plan: A document prepared in accordance with the principles of HACCP to ensure control of hazards which are significant for food safety throughout the food chain under consideration.

Haemagglutinins: A group of proteins of plants that are not antibodies and do not originate in an immune system but bind specifically to carbohydrate containing receptors on cell surfaces (red blood cells). It is also known as Phytoagglutins / lectins.

Galactan: It is a polysaccharide in which major monosaccharide subunit is galactose and often found among the pectic substances of cell walls.

Galactoblast: It is also called colostrums. It is female’s first milk after parturition.

Galactometer: It is an instrument used for recording the specific gravity of the milk.

Galactorrhea: A spontaneous flow of milk from the nipple is called galactorrhea.

Galactosamine: An amino derivative C6

Galactose: It is a reducing sugar and is monosaccharide. It is an aldohexose sugar obtained by hydrolysis of lactose.

Galactosidase: An enzyme (actase) that hydrolyzes a galactoside.

Galactoside: A glycoside that yields galactose on hydrolysis.

Galactosyl: A glycosyl radical C6 H11 O5 that is derived from galactose.

Galacturonic Acid: A crystalline aldehyde acid C6 H10 O7 that occurs in polymerized form in pectin.

F- distribution: A probability density function that is used in analysis of variance and is a function of the ratio of two independent random variables each of which has a chi square distribution and is divided by its number of degrees of freedom.

F0 value: It is used to express F-value when z =10oC / 18oF and the temperature is 121.1oC. It is the number of minutes at a specific temperature (250oC) required to destroy or kill a stated number of microorganisms and has specific z-value. It is also called reference value.

Facing or lining board: The layer of the paper on which the flute is embedded is called facing or lining board (Plate 151).

Factor VIII: It is a glycoprotein based clotting factor of blood plasma that is essential for blood clotting and is absent or inactive in hemophilic patients. It is also called alsoantihemophilic factor

Facultative: Possessing the capacity to survive in more than one type of environment.

Faculative anaerobeic: Organisms that grow either with or without oxygen.

Facultative parasites: Facultative parasites can adapt to live on dead organic matter and often have a wide host range and can easily be cultured on artificial media. These organisms may be aerobic or anaerobic but under changed conditions, they changes to anaerobic or aerobic.

E numbers: Each food additive used in the food industry has been given a specific number code for their identification. These numbers are called as E numbers. These E numbers are used within the European Union. The word prefix “E” stands for Europe. They are commonly used on the food label through out the European Union. These are not used in Australia and New Zealand. The different food additives are given different E numbers depending upon the type of food additive like E100-E199 (food colours), E200-E299 (preservative), E300-E399 (antioxidants), E400-E499 (thickners, stabilizers, emulsifier), E500-E599 (pH regulator, anticaking agent), E600- E699 (flavour enhancers), E700-E799 (antibiotics), E900-E999 (miscellaneous like waxes, synthetic glazes, packaging gases, sweetners, foaming agents) and E1000-E1099 (additional chemicals).

Eovalue: It is the thermodynamic favourability of the reaction.

Dahi: It is the product obtained from pasteurized or boiled milk by souring, natural or by harmless lactic acid or other bacterial culture. It is also called Curd (Plate 126).

Daidzein: These are the substance from which daidzein is derived and are isoflavone C15 H10 O4 found chiefly in legumes and esp. soybeans.

Dairy: It is a farm building often a single room in which milk is cooled and temporarily stored prior to collection for transportation to the commercial diary. Here the milk is treated and made into cream, butter and cheese. Or

Dairy: It is a farm building often a single room, in which the milk collected is pooled, temporarily stored prior to collection for retailing or direct sale to the consumer. OrDairy: It is a room, building, or establishment where milk is kept and butter / cheese is made or it is the department of farming / farm that is concerned with the production of milk, butter, and cheese oran establishment for the sale / and distribution of milk and milk products.

Dairy farm: It is a farm on which the principal activity is the milk production.

Dairy husbandry: It deals with care, breeding, feeding, milking of the diary cattle and the production and sale of the milk. It is the economical management of a dairy, including looking after the dairy cattle and proper disposal of milk and milk products.

Dairy products: These are the milk preparation, including butter, cream, cheese, ghee, yoghurt, pasteurized milk, tonned milk, butter milk etc processed and prepared in dairy. Ice cream and kulfi or sweets are not included in this.

Damage: Damage is a physical spoilage, often a partial deterioration, are subjectively judge and very difficult to measure and usually reported as % of the sample. Daminozide: It is a growth regulator which is used for red colour development in apple.

C - enamel can: The sulphur resistant lacquer is golden coloured and the can coated with it are called C-enamel can or sulphure resistant can, used for packing non acid products (Plate 81).

CA storage: Storage of commodities under gaseous atmosphere like gas storage, where the composition of atmosphere is controlled accurately. MA and CA differ only in degree of control and CA is more exact. These storage methods in combination with refrigeration markedly enhance storage life of fruit and vegetables (Plate 82).

CAC: It stands for Codex Alimentarious commission (1962). It is a joint commission of FAO and WHO, comprising of 146 member countries. It ensures consumer food safety, establish fair practices in food trade and promote the development of international food standards. CAC make standards for food additives, veterinary drugs, pesticide residue and other miscellaneous substances that affect consumer safety. These are published in Codex Alimentarius.

Cacao: The dried partly fermented fatty seeds of Theobroma cacao of the family Sterculiaceae that are used in making cocoa, chocolate, and cocoa butter called also cacao bean (Plate 83).

CAD: CAD (computer-aided design) is the use of computer-based software to aid in design processes. CAD software is frequently used by different types of engineers and designers. CAD software can be used to create two-dimensional (2-D) drawings or three-dimensional (3-D) models (Plate 84).

B cell: It is one of the lymphocytes that have antigen-binding antibody molecules on the surface, that comprise the antibody-secreting plasma cells when mature, and that in mammals differentiate in the bone marrow. It is also called B lymphocyte.

B.T.U.: It stands for British thermal unit. The heat required to raise the temperature of one pond of the water through 1oF is called BTU.

Baby back ribs: Meaty pork ribs cut from the lower back rib section.

Bacillary dysentery: It is a food borne illness characterized by very diarrhea and electrolyte loss alongwith intense abdominal pain, high fever and even death.

Bacillus spp: The genus Bacillus is generally aerobic, spore forming. Bacillus cereus also produces 2 types of toxins; a very fast aching emitting toxin which cause vomiting and a diarrhoeal toxin. Bacillus is a genus (Bacillus) of straight rod-shaped gram-positive usually aerobic bacteria producing endospores and including many saprophytes and some parasites produces disease (B. anthracis of anthrax).

Bacitracin: A polypeptide antibiotic isolated from a bacillus (Bacillus subtilis or B. licheniformis) and usually used against gram-positive bacteria.

Back Bacon / Canadianbacon: Bacon cut from the loin that has little fat and is cut into round or oblong slices.

Backward feed evaporator: In this evaporator, fresh feed enters the last and the coldest effect and continues on until the concentrated product leaves the first effect (Plate 54).

Bacon: Bacon is the meat cut from belly portion of hog carcass (a domestic swine esp. when weighing more than 120 pounds or 54 kilograms) and has high percentage of fat. It is cured and smoked.

Abattoir: An establishment where animals are butchered is known as abattoir. It is also known as slaughterhouse.

Abiotic: Abiotic means any factor which is not biological / a biological and especially there is no involvement of the organisms in synthesis of certain compounds.

 Abnormal milk: It is milk that is not smooth and homogenous but is flaky, ropy, stringy, slimy, water, discoloured or bad flavoured. It may be due to animal sickness or disease or due to excessive medicines given or uncleaned milk condition.

 Abomasum: It is the fourth stomach of the Ruminants which is lying close to the

Omasum or the third stomach, in which digestion gets completed by the supply of the digestive juice. It is also called Reed stomach, rennet stomach, rennet-bag, or reed

tripe and maw (Plate 1).

 Abrassive peeler: It is equipment comprising a rotating drum lined with carborandum (an abrasive material) in which certain vegetables like potato and ginger are tumbled and exposed to the abrasive action of the carborandum lining, thereby facilitating separation of peel from the vegetables (Plate 2).

Food Business Regulatory System in India

In India Food Businesses are regulated by only One authority i.e. Food Safety and Standards Authority of India (FSSAI). This comes under the control of Ministry of Health and Family Welfare of the Govt. of India.

FSSAI – Food Safety and Standards Authority of India

The FSSAI or Food Safety and Standards Authority of India was incorporated under the Food Safety and Standards Act (FSSA) of 2006. It consolidated various acts & orders that were previously applicable and used to handle food related issues in different Ministries and Departments. The basic objective of formulating FSSAI has been to lay down science-based standards for items of food and to regulate their manufacture, storage, sale, distribution, and import to ensure the availability of adulteration-free and wholesome food for human consumption.

Introduction

Citrus fruits are acidic, exotic fruits with juicy, bitter, fruit segments inside. Citrus fruits commonly originated in tropical and sub-tropical regions of the world. They have long been valued as part of a nutritious and tasty diet. The f lavours provided by citrus are among the most preferred in the world, and it is increasingly evident that citrus not only tastes good, but is also good for people. Citrus fruits have certain therapeutic properties such as antitumor, anti-inflammatory and anticancer, due to phytovitamins and nutrients present in them.

Introduction

It is the process of retaining food over a period of time without being contaminated by pathogenic organisms or chemicals and without losing its colour, texture, flavour and nutritious value. It involves preventing the growth of bacteria, fungi and other microorganisms, as well as retarding the oxidation of fats which causes rancidity

Objective

• To increase its shelf life, retaining original nutritional values, colour, texture, and favour.

The production of fruit and vegetables has recently increased significantly and even surpassed the total grain production in the country due to increased awareness of consumer and positive government initiatives (Schieber et al., 2001; Sagar et al., 2018; Malenica and Bhat, 2020). But due to limited production and area, the importance of excess production during a particular season and waste management arises at highest priority level. Moreover, fruits and vegetables waste are a serious problem for environment as most of themare being disposed of as landfills or directly disposed off to water bodies such as rivers (Wadhwa and Bakshi, 2013). Fruits and vegetables are perishable in nature and rich source of nutrients and vitamins, which results in microbiological contamination (Coman et al., 2019; Bas-Bellver et al., 2020). Foods wastes are usually organic residues generated by the processing of raw agricultural materials into food and are made up of liquid (wastewater) and solids (Bas-Bellver et al., 2020). The wastewater results from the cleaning processes or in the form of excessive or polluted process water. Its dry material content is typically less than 5% by mass. It possibly also contains organic or inorganic cleaning agents or disinfectants. Solid food wastes with an organic origin have remarkably high water content (mostly about 80% by mass). They are usually characterized by a constant quality and purity due to the forgone processes. The fact that these substances are removed from the production process as undesirable ingredients makes them, wastes.

Introduction

Increased awareness about the food safety and various adulterations or crises reported in food industries, the food quality and its safety has emerged as the most important features of the modern food processing and preservation. The important historical dynamics which raised the concerns of food safety were motivated by accidents related to food borne pathogens and chemical contamination during the food processing and distributions. Many times, the terms food quality and safety are interchanged however, there is a difference in terms of their perception by the consumers and manufactures. In past few years, the food safety has become the characteristic of food quality and has been regulated by various national and international agencies. The increased concerns about the safe, nutritious and high quality food has over the time forced the public authorities to engage the food industries to construct the quality management systems in order to improve the food safety and build the consumers believe and trust towards processed foods. Food quality is a broader concept which includes food health quality attributes along with parameters like sensory characteristics like taste, smell and convenience like food preparation (Röhr et al., 2005). The food qualities like nutritional, convenience and sensory are not requested by the public laws but are desired by the consumers. The management systems for the quality assurance have evolved over the time and now we have systems like HACCP. ISO 9000 and ISO 14000 in place which are well known quality management modules to ensure the elimination of food hazards. On a broader scale of trading food several technical agreements under WTO (world trade organization) have been made which provide equal opportunities to all countries to trade the goods however, it imposes the similar food quality standards across all the participatory members. The responsibility of food safety is directly connected with all the stakeholders of the food chain which include manufactures, processors, farmers, food handlers and finally the consumers. Food contaminations may be a result of poor manufacturing practices and poor storage and handling practices at any stage in the food chain. To ensure the safety compliances, national food regulatory models plays a significant role. The guidelines issued by FAO/WHO, 2003 are focused on the public organizations, as well as food control authority self-assessment to ensure highest food quality and its safety from farm to fork. The base of the assessment includes 6 factors viz. food inspection, food quality management, food safety, official food control laboratories, food legislation food and quality information, education and communication (Shukla et al., 2014).For effective implementation of the food safety and quality module the countries have developed various food laws and legal norms which states the principles to be followed for raw materials, their processing and distribution in order to achieve the consumer expectations as well as the hidden food safety concerns.

Introduction

How one can differentiates between sensory science and sensory evaluation? Perhaps the simplest way would be to think of sensory science as the fundamental physiological and psychological underpinnings of sensory evaluation. It could include our basic understandings of the mechanisms of taste and odour, kinesthetic and the various techniques of scaling and statistical analysis, whereas, sensory evaluation could designate the application of this knowledge and the development of specific methods for the evaluation of the discriminative, descriptive and affective nature of products, in a problem-solving situation. When the quantitative descriptive analysis (QDA) methodology was f irst described, it represented a decade of experimentation and study of the issue that were perceived at the time to be impediments to solving “the problem of flavour characterization” (Stone et al., 1974). As noted by Pangborn (1964), new developments and knowledge about sensory evaluation were beginning to have a positive effect on its acceptance by industry, but much more needed to be done if it was to contribute in a meaningful way. Today, it is indisputable that flavour is an important attribute affecting consumer acceptance of food. “Flavour is taste plus odour”. The perception of taste is merely the detection of non-volative tastants by the tongue. On the other word, the perception of odour is the detection of odoriferous volatile aroma compounds by the olfactory nerves ending in the nose. The combination of all possible flavour stimuli we could encounter is as vast as the galaxy. However, most of the attempts to define these experiences have historically been over simplified.

Introduction

Packaging is one of the most important processes to maintain the quality of food products for storage, transportation and end-use. The basic functions of packaging are considered as protection, containment, information and convenience. Food packaging was initially considered to increase the marketable life of the food in a container by protecting it from the influence of the environmental factors. Then it was expected to be informative, easy-to handle and disposal-friendly with increased functionality. Current innovations of food packaging technology are geared to ensure one/ all of these properties along with better protection, more efficient quality preservation and enhanced safety of the packaged food. Innovative packaging technologiesalso prevent the rate of quality deterioration and facilitate easy handling of the produce during distribution and marketing. During distribution, the quality of the food product can deteriorate biologically and chemically as well as physically. Innovative packaging technologies are the most important and challenging technologies to overcome these limitations.These technologies require specific knowledge and appropriate training and understanding for rationally selecting the most suitable packaging for each product and intended use. Innovative packaging of foods includes active packaging, smart packaging, on-the-go packaging, intelligent packaging etc. which have been briefly discussed in this manuscript.

Introduction

Color is an important characteristic of food. Based on the color of the food, f irst impressions are made: Is the fruit immature, ripe, or overripe? Is the toast burnt? Is the food fresh? Based on these first impressions, a judgment is made whether the food is safe to eat or not and whether it can be expected to taste good or not. Since color is closely associated with expectations, the addition of color to food is a way to fulfill these expectations. Color is added to food for one or more of the following reasons:

1. To replace color lost during processing,

2. To enhance color already present,

3. To minimize batch-to batch variations, and

4. To color otherwise uncolored food.

Introduction

Fermentation is playing an important role in the human life and is evident from the fact that food can be both spoiled and made by fermentation. A wide range of the fermented food products are consumed by the consumers in their daily routine. It is one for the oldest and ancient method of food preservation. It may have been a mere accident when people first experienced the taste of fermented food. Fermentation became popular with the dawn of civilization because it not only preserved food but also gave it a variety of tastes, forms, and other sensory sensations. Fermentation, along with salting, cooking, smoking, and sun drying, is one of the earliest ancient traditions developed by cultures all around the world to extend the possible storage time of foods. Slowly, people have realized the nutritional and therapeutic value of fermented foods and drinks, and this has made fermented foods even more popular. Bread, beer, wine, and cheese originated long before Christ. Although modern food technology has contributed to the present day high standard of quality and hygiene of fermented foods, the principles of the age-old processes have hardly changed. In industrialized societies, a variety of fermented foods are very popular with consumers because of their attractive flavor and their nutritional value. During food fermentations, the controlled action of selected micro-organisms is used to alter the texture of foods, preserve foods by production of acids or alcohol, or to produce subtle f lavours and aromas which increase the quality and value of raw materials. After drying, fermentation is the world’s oldest food preservation method.

Introduction

The process of concentrating a fruit juice / pulp, vegetable juice, medicinal plant extracts or milk to a reasonably thick consistency is called concentration. Thus, concentration is a water removal process from the liquid food materials. The basic principle of concentration is boiling the liquid under partial vacuum at low temperature till desired concentration is reached. With respect to water removal, the concentration process is similar to dehydration. The food concentration is distinguished from drying on the basis of the finished product where the final product called concentrate is still liquid. In concentration process, only liquid food is used while in drying both liquid as well as solid food can be used. Fruit juice concentrates, milk concentrate are valuable semi-processed products for use in the production of value added products like fruit juice beverages and fruit juice powders. Since the water content of single strength fruit juices is very high (about 75-90%), concentration of fruit juices not only provides microbiological stability but also permits economy in packaging transportation, and distribution of the finished product due to reduction in bulk by weight and volume.

Introduction

The drying of fruits and vegetables during the season of abundance in order to preserve them for consumption during season of shortage is an ancient practice. Thus, drying is one of the oldest methods of preservation of the food commodities. It is practiced from ancient times and is a very common method of preservation in the tropical countries like India where conditions are much more favorable for sun drying. All micro-organisms need moisture to grow. So the removal of water in the food is brought down below a certain level at which they are unable to grow. Moisture content from food can be removed by different methods like sun drying (very oldest method and most popular), solar drying, cabinet drying, mechanical drying, tunnel drying, poly tunnel drying, adiabatic drying, spray drying, osmotic drying, fluidized bed drying etc. Grains are mostly dried in the sun. The moisture content of the grains can be reduced to a limit that is safer for their storage by sun drying. Many fruits and vegetables can also be dried by using sun energy. Some coastal areas of the countries fishes are also dried by exposing to the sun. After drying, sometimes the dried products are converted into powder. However, these powders are very hygroscopic and should be packed in airtight. Mango powder is prepared by this technique, but expensive and not popular in India. Nowadays many artificial methods have been developed for removal of the moisture. The relationship between the moisture content and temperature of air during process is referred as Psychrometric relation. An instrument used to measure the humidity (Relative humidity) of the atmosphere is known as Psychrometer (Hygrometer). The residual moisture in vegetable should not be more than 6-8% and in fruits it should be in the range of 10-20%. The dried fruit can be used as such or after soaking while dried vegetable are usually soaked in water overnight and then cooked. Vegetable are considered to be dry when they become brittle. There are generally two drying methods for foods like 1) Natural drying and 2) Artificial drying. The preservation / stability of the dehydrated products is conferred by the abstraction of water, so that the product is unable to support the microbial growth, its chemical forms of deterioration is inhibited and the drastic reduction in the weight / volume facilitate easier handling and marketing. Among dehydrated products, dehydrated onions have largest share of 45%. India is the third largest exporter of the onions in the world next to the Netherland and Spain. Besides this, India exports dehydrated bell pepper, garlic and mushrooms.

Introduction

Drying is one of the oldest methods of preserving food. Drying and dehydration both can be defined as the process of removing moisture from the foods. It is a simultaneous heat and mass transfer process, wherein heating medium or internal heat generation helps in evaporation of free water molecules from the product. Drying means removal of moisture of food upto a predetermined level where as in dehydration, complete removal of moisture upto bone dry weight of food material. Dehydrated foods have less than 2.5 % moisture content (dry basis) where as dried foods have dry basis moisture content more than 2.5 %. Drying operation consumes energy from 0.38 to 0.63 GJ/ton of food grain. Removal of water from foods using thermal energy thus makes it an energy intensive process. Modes of heat Transfer in various Driers are given in Table 1.Developments in dryer and methods, dryer selection based on form of raw material and typical residence time for various dryer are are given in Table 2,3 and 4, respectively.

Introduction

It is the process of retaining food over a period of time without being contaminated by pathogenic organisms or chemicals and without losing its colour, texture, flavour and nutritious value. It involves preventing the growth of bacteria, fungi and other microorganisms, as well as retarding the oxidation of fats which causes rancidity.

Objective

• To increase its shelf life, retaining original nutritional values, colour, texture, and favour.

Introduction

The health and wellness of human being is largely dictated by the consumption of nutritious foods. Understanding the role of foods in maintaining health and cure of diseases has its roots in the ancient Hippocratic and Vedic texts as well as in the canons of Traditional Chinese Medicine (Sharma et al., 2012). Presently, the transition from a traditional to modern lifestyle, consumption of diets rich in fat and calories combined with a high level of mental stress has been the major cause of lifestyle related diseases like coronary heart disease, obesity, diabetes, cancer and hypertension (Moczara et al., 2012; Sharma et al., 2018). Various studies have linked foods as helpful in combating a number of such degenerative diseases (Sharma, 2015).Therefore, the tenet "Let food be thy medicine and medicine be thy food", quoted by Hippocrates about 2,500 years ago is receiving overwhelming interest. There are number of scientific reports emphasizing the importance of consuming biologically active ingredients present in fruits, vegetables, whole grains and legumes to reduce the risk of chronic diseases (Hasler 2002; Das et al., 2011; Sharma and Sharma 2016). Such biologically active ingredients are now popularly known as neutraceuticals. Whereas, the functional foods are the foods that resemble traditional foods but possess demonstrated physiological benefits. In some countries, however, functional foods and nutraceuticals are used interchangeably. Regardless, the main focus of such products is to improve health and reduce disease risk through prevention. The main difference of this category with pharmaceuticals is that they are multi-targeted mixtures and present at low concentration while pharmaceuticals are uni-targeted pure compounds with high dose use. Hence, an overview on the concept of nutraceutical and functional foods, their classification, development and regulations is summarized in this article.

Need to Preserve Food

Food is vital for human existence. Conservation and preservation of food is a prerequisite for food security and it provides economic stability and self reliance to a nation. The need to preserve food has been felt by mankind since time immemorial. The seasonal nature of production, long distances between production and consumption centers and rising gap between demand and supply have made this need even more relevant today. The hot and humid climate of a country like India is quite favorable for the growth of numerous insects and microorganisms that destroy stored crops and cause spoilage of food every year. Spoilage can also occur due to chemical and physiological changes in stored foods. Sea-foods, meat and poultry may carry harmful microbes and parasitic organisms that cause illnesses associated with their consumption. As in other parts of the world, India has also practiced various methods of food preservation such as sun drying, pickling and fermentation which were supplemented with more energy consuming techniques such as refrigeration, freezing and canning. Each of these methods has its merits and limitations. Man has always been on the search for newer methods to preserve foods with least change in sensory qualities. Radiation processing of food is one of the latest methods developed for this purpose.

Introduction

In this modern era, educated and health-conscious people are increasingly demanding such food products that preserve their nutritional value, retain a natural and fresh colour, flavour and texture, and free from chemical preservatives. Besides, urbanization has extensively affected the life-style of people all over the world. Competition has made their life extremely busy and time has become a very important factor. Hence, recently there has been increase in demand for ready-to-cook or ready-to-use fresh food products of fruits and vegetables along with convenience and safety. This new demand by consumers has opened a new avenue of food preservation known as minimal processing. The products prepared by this processing method are known as fresh cut or pre-pared products. Minimally processed products are also known as lightly processed, partially processed, fresh processed, fresh cut, and pre prepared. It can be defined in many ways for example as "least possible treatment to achieve a purpose". A more specific definition is as the method that "preserve foods but also retain to a greater extent their nutritional quality and sensory characteristics by reducing the reliance on heat as the main preservative action. In a broader way it can also be defined as the processed foods have been slightly altered for the main purpose of preservation but which does not substantially change the nutritional content of the food. Generally minimal processing involves cleaning, washing, trimming, coring, slicing, shredding before packaging and storage at specific temperature. Some of the examples of minimally processed fruits and vegetables include sliced mango cheeks/melons/papaya/apple, pieces of pomelo segments, peeled and cored pineapple, chilled peach, pomegranate arils dressing, shredded lettuce, shelled peas, cauliflower florets, peeled sliced potatoes, diced onion etc. Minimally processed products are important to food service industry such as eateries and catering companies as they offer many advantages over traditional products, with respect to convenience, expense, labor, and hygiene. Even if it is very much liked by people, the production of minimal processed products is limited due to rapid deterioration and short shelf life. Whereas most food processing methods stabilize the products and prolong their shelf-life, minimal processing of fruits and vegetables increases their perishability.