eChapters

Abstract

The applications of enzymes are awe inspiring marvels of nature. Scientists can use enzymes to explore all aspects of biology; from molecules to cells to tissues to organs to organism as a whole. For the purpose of food and drinks, enzymes have been exploited by men for thousands of years without really knowing what they are and how do they work? Enzymes have played a pivotal role in many aspects of life since the dawn of life. Today, biocatalysis is being used as a useful alternative to chemical process technology. The fact that enzymes work under much milder conditions oftemperature, pressure and acidity, thus, obviate harsh processing conditions and thereby decrease the possibility of damage to heat labile substrates and save energy. Enzyme technology being eco-friendly can be justifiably termed as‘Green technology’. Due to their high specificity and high catalytic rate, enzymes prevent wastage and reduce time of processing / manufacturing, respectively. The ability of enzymes to act on wide variety of substrates has made them useful tools to exploit cheaper starting material in food industry. Reportedly, over 45% of the enzymes produced are being used in food industry and remaining is shared by detergent (34.4%), textile (11%), leather (2.8%) paper and pulp (1.2%) and other industries (5.6%). Enzymes serve a myriad of functions in food processing, from maximizing juice extraction of pectin-rich fruits to extending shelf-life in baked goods through slowing starch retro-gradation. Indian food processing industry has seen significant growth and changes over the past few years. Though, the market of enzymes is increasing at a fast pace but most of the enzymes of the food sector are coming from overseas. Seeing the unparallel role of enzymes in food processing sector as in multifaceted domains, the researchers should emphasize upon finding novel enzymes and explore their untapped commercial benefits using modern techniques of enzyme engineering, immobilization and biotechnological interventions.

Abstract

Meat is considered to be a highly perishable food commodity because of its biological composition. Thus, to extend its shelf life and maintain its nutritive value, there is an urgent need to find out alternatives for synthetic anti-microbial and anti-oxidants. This will not only serve the purpose of meat product manufacturer’s but will also satisfy the consumers’ demand for minimally processed meat products devoid of synthetic preservatives. This review discusses the natural antimicrobial and anti- oxidants sources viz., various essential oils, their composition and application in different meat and meat products. Essential oils from basil, rosemary, thyme, oregano, ginger, basilica, balm, coriander, rosemary and clove have exhibited a better potential to be used as an antimicrobial agent in meat and meat products. They owe their anti-microbial properties to the phenolic compounds present in them. They dislocate cell membrane and disrupt functionality of bacterial cell membrane and eventually leading to the release of intracellular components. However, essential oils are not widely used due to the pungent and intense aroma imparted to the food, thus there is still need to work out on the novel technologies which can not only increase food shelf life but at the same time, are sensorially acceptable to the consumers.

Introduction

Fermentation has been an integral part of food processing throughout the history of mankind. In beginning, it was mainly preservation method, then it became key process in substantial sensory characteristic development and now it is utilized to improve overall properties of outcome from major industries like bakery, dairy, beverages, etc. The human understanding of controlled fermentation process changes its fundamental objective by exploring its success in development of rheological and texture characteristics.

The biochemical transformation of raw food due to fermentation affects the organoleptic as well as rheological properties of a product. The changes in rheological as well as sensory properties is related with the modification of major component by the action of microorganism. For example, transformation of polysaccharides in bread dough by action of yeast produce ethanol, CO₂ and other simple compound which modify sensory as well as rheology of dough. The major micro-structural, bio-compositional and bio-chemical changes are due to microbial action are frequently described to understand the effect of fermentation on rheological as well as sensory properties of food products. The rate and quality of these associated major changes mainly depends upon concentration of saccharides for growth of microorganism as well as nature and existing microflorae. Hence, a proper monitoring of changes in rheological and sensory properties is necessary to optimize the controlled fermentation as well as design of process parameters from engineering and consumer point of view.

The biochemical changes directly responsible for changes in basic rheological and sensory characteristic including flow behavior, consistency, viscoelastic properties, hardness, adhesiveness, color, aroma, flavor and mouthfeel. The food industry is so diverse and exploring new and possibilities to catch diverse consumer worldwide, a proper understanding of effect of fermentation on mentioned properties will be key to hold competitive market. Hence, the present chapter will cover the basics of rheology and its role in food industry, effect of fermentation on rheological and sensory attributes of major class of food.

Abstract

Dietary fibers mostly include non-starch polysaccharides such as celluloses, hemicelluloses, gums and pectins, lignin, resistant dextrins and resistant starches. Based on their water solubility, dietary fibers may be divided into two forms insoluble dietary fiber which includes celluloses, some hemicelluloses and lignins which is fermented to a limited extent in the colon and soluble dietary fiber which includes f-glucans, pectins, gums, mucilages and hemicelluloses that are fermented in the colon. Resistant starch generally considered to be represented by the total amount ofstarch and the product of starch degradation that are not digested in the small intestine andpass into large intestine, and regarded as a component ofdietary fiber. Herbal nutraceutical is used as a powerful instrument in maintaining health and to act against nutritionally induced acute and chronic diseases, thereby promoting optimal health, and quality of life. Dietary spices in their minute quantities has an immense influence on the human health by their antioxidative, chemopreventive, antimutagenic, anti-inflammatory, immune modulatory effects on cells and a wide range of beneficial effects on human health. Nutraceutical is the hybrid of ‘nutrition’ and ‘pharmaceutical’. Nutraceuticals, in broad, are food or part of food playing a significant role in modifying and maintaining normal physiological function that maintains healthy human beings. Functional foods are including whole foods and fortified, enriched, or enhanced foods or dietary components that may reduce the risk of chronic disease and provide a health and physiological benefit beyond the traditional nutrients it contains.

SYNOPSIS

Fish has now become an important animal protein for mankind. It is a rich source of essential amino acids, vitamins and minerals. Fish meat is easily digestible compared to other animal meat and has a higher Protein Efficiency Ratio when compared to casein, the milk protein. Fish oils like body oils of fishes like sardine, mackeral, anchoviella, herring, salmon etc. are the richest source for Omega-3 long chain poly unsaturate fatty acid like Eicos- pentanoic acid (EPA) and Docosahexaenoic acid (DHA) having therapeutical applications for man. A number of fishery products and by-products like squalene, chitin, chitosan, oyster meat, mussel meat, shark bones etc.

3.1 INTRODUCTION

Life cannot be sustained without adequate nourishment. Human beings need adequate food for growth, development and to lead an active and healthy life. Animals satisfy basic food requirement mainly through natural selection. But human beings have access to a wide range of foods to choose to make up their diet. Since all foods are not of same quality from nutritional point of view, man’s ability to meet his nutritional need and maintenance of good health depend upon type and quantity of foods he is able to include in his diet to satisfy hunger. Human beings need a wide range of nutrients to perform various functions in the body and to lead a healthy life. Several reports have shown that adequate intake of fruits and vegetables forms an important part of a healthy diet and low fruit and vegetable intake constitutes a risk factor for chronic diseases such as cancer, coronary heart disease (CHD), stroke and cataract formation. The nutrients include proteins, fats, carbohydrates, vitamins and minerals. The foods containing these nutrients which we consume daily are classified as cereals, legumes, nuts and oil seeds, fruits, vegetables, milk and milk products and f lesh foods like fish, meat and poultry. Some foods provide only a single nutrient and some multiples. Scientific evidence indicates that frequent consumption of fruits and vegetables can prevent oesophageal, stomach, pancreatic, bladder and cervical cancers and that a diet high in fruits and vegetables could prevent 20% of most types of cancers. Reports indicate that increasing individual fruit and vegetable consumption by 600 g per day could reduce the global burden of stroke by 19% and decrease the risk of coronary heart diseases by 31% respectively. Realizing the food value of fruits and vegetables in human nutrition the Indian Council of Medical Research (ICMR) recommends per capita use of 120g fruits and 280g vegetables per day.

Abstract
The United Nations predicts that by 2050, the global population will reach 9.6 billion, leading to urgent challenges such as ensuring food security and reducing greenhouse gas emissions. Climate change, coupled with biotic and abiotic pressures, will significantly impact global food production. Despite advances in agriculture, malnutrition remains a widespread issue, affecting millions, especially in developing countries. Protein-energy malnutrition, which affects over 170 million children and women, poses a significant threat to national economic development, increasing healthcare costs and reducing productivity. One key solution to combating malnutrition lies in promoting legumes (pulses), which are nutrient-dense, protein-rich, and vital for both human and animal consumption. Pulses have been a staple in diets
for centuries, providing essential nutrients like protein, fiber, vitamins, and minerals. They are not only beneficial for human health, reducing the risk of heart disease, diabetes, and cancer, but they also contribute to sustainable agriculture. Legumes can fix atmospheric nitrogen, reducing the need for synthetic fertilizers, improving soil health, and increasing agricultural productivity. However, despite their benefits, pulse production lags behind other staple crops like cereals. With proper management, legumes could play a vital role in alleviating global hunger, particularly in developing countries. Increased awareness and efforts to incorporate legumes into farming systems and daily diets could help meet the growing global demand for nutritious, sustainable food.
Keywords: Climate change, Food security, Global hunger, Legumes, Proteinenergy malnutrition, Sustainable agriculture

Millions of people worldwide suffer from food insecurity and malnutrition, which are exacerbated by issues including pollution, overcrowding, climate change, and economic hardship. These problems highlight the necessity of changing food systems for better immunity and health since they lead to both undernutrition and overnutrition. Essential carbs, fiber, fats, and important micronutrients including iron, calcium, potassium, zinc, and magnesium are all found in nutrient-dense grains such as foxtail, kodo, proso, tiny, and pearl millets. These millets are widely accessible and have several health advantages. For example, pearl millet is high in amylose starch and contains essential minerals like phosphorus and magnesium, whereas proso millet is a good source of iron, calcium, potassium, and B-vitamins. B vitamins, iron, calcium, and zinc are all found in kodo millet, while finger millet is a great provider of calcium, iron and magnesium. The nutritional profile of millets is further improved by processing methods like milling, germination, extrusion, and fermentation. Prominent research organizations, including as ICRISAT, ICAR, and CGIAR, are actively working to conserve millet and create better types to combat chronic illnesses and malnutrition. Targeted improvements in disease resistance and nutrient content are now possible because to biotechnology advancements. For improved worldwide health outcomes, dietary patterns must nonetheless be changed and these nutrient rich grains incorporated into contemporary food systems, notwithstanding current advancements.

Abstract

Nanotechnology deals with understanding and control of matter at 1-100 nm dimensions. Nano-sized materials have been part of everyday life in the form of biological entities and processes. Many of the structural building elements in foods are colloids in nature and are built up as a result of self assembly of nano-sized molecules in particles or at interfaces. The most important food raw materials-proteins, starches and fats undergo structural changes at the nanometer and micrometer scales during normal food processing. Unique phenomena occurring at this length scale are being exploited for novel food applications. Functional foods are products, in food or drink form, which influence specific functions in the body and thereby offer benefits for health, well-being, or performance beyond their regular nutritional value. Nanotechnology promises to provide means of altering and manipulating food products to efficiently deliver nutrients like proteins and antioxidants for precisely targeted nutritional and health benefits. Nanotechnology applications for functional food ingredients and additives offer range of benefits to the consumer in terms of innovative, tasteful and healthy food products. Nanoencapsulation systems aid smart protection and delivery of bioactives so as to ensure their stability, taste, colour, safety and bioavailability. The advent of nanotechnology has opened up innovations in food packaging with improved stability and barrier properties. However, need exists to develop regulatory systems for managing any risks associated with functional nanofoods and the use of nanotechnologies in the food industry.

In this century the usage of plastics had become very common. However, it is important to know as to what kinds of plastics are used for food handling and storage and the health hazards caused by using it. It has been observed that plastic packaging plays a significant role for the enhanced shelf life and in the ease of storage and cooking for many foods. Some kinds of plastic materials which are widely used for handling and storage of food and water include polyethylene terephthalate (PET). It is used in soft drinks, water, sport drinks, ketchups, salad dressing bottles, peanut butter, pickle, jelly and jam jars. This plastic is strong, heat resistant, resistant to gases and acidic foods. It can be transparent or opaque. It does not leach any chemicals that are suspected of causing cancer or disrupting hormones and can also be recycled.

Usage of High density polyethylene (HDPE) has been found to be common in milk, water, juice bottles, yogurt, margarine tubs, grocery, trash and retail bags. High-density polyethylene is stiff and strong but is not heat stable (it melts at a low temperature). It does not leach any chemicals that have a role in causing cancer or disrupting the hormones. HDPE can be recycled. Low-density polyethylene (LDPE) is used into making films of various types, some bread and frozen food bags and also in the making of squeezable bottles. Low-density polyethylene is relatively transparent. Many of the films are not heat stable either and may melt the food in case it is touching it. However, polypropylene (PP) is more heat resistant, harder, denser and more transparent than polyethylene and is therefore used for heat-resistant microwavable packaging and sauce or salad dressing bottles. Polycarbonate is clear and heat resistant. It is also durable and often used to make refillable water bottles and sterilisable baby bottles, microwave ovenware, eating utensils, plastic coating for metal cans. Tiny amounts of bisphenol A are formed when polycarbonate bottles are washed with harsh detergents or bleach (sodium hypochlorite). At high levels of exposure, bisphenol A is potentially hazardous as it mimics the female hormone estrogen. In addition, polystyrene (PS) and polyvinyl chloride (PVC) are also used during food material transportation and handling in supermarkets. Modern food safe plastic bags are plasticizer-free which do not leach harmful chemicals into the food during cooking.

Probiotics are measured in colony forming units (cfu). CFUs are generally measured in the millions or billions per serving. Probiotics are most commonly beneficial bacteria, but can also be friendly fungal or other organisms, that are typically freeze dried to stabilize them in an inert state during storage and production. Then their continued stability and viability, as measured by CFU counts when cultured, is dependent on limiting their exposure to stimulating environmental conditions such as temperature and moisture. Besides refrigeration, this protection can be done by packaging in glass or by adding freshness packets that helps to absorb and reduce moisture in the package.

Positivism emerged out of the modernist era, which experienced its fullest expression during the Enlightenment of the 18th century, but which had been stimulated through the earlier writings of philosophers such as August Comte, John Stuart Mill, and David Hume. Modernists believed that through the systematic application of logical mental process, ignorance and superstition could be replaced by progress, justice, and beauty. The tremendous optimism of the modernistic era was accompanied by an unrelenting faith in both the epistemology and methodology of positivism.

The foundation of positivism was grounded in a mechanistic worldview based on the assumption of predictable cause-and-effect patterns of relationships. Furthermore, positivism was grounded in the belief that there exist some laws in the natural and social worlds that are unaffected by one’s perspective and that can best be understood through reliance on objective scientific. Positivism led to positive thinking, positive attitude, and positive actions. Positive thinking leads to good mental and emotional health. So, there is a strong relationship between mental health and the role of positive thinking.

Introduction

Since the last century, nutritional science has made a remarkable contribution to the biological sciences through identification of diverse nutrients and their sources, functions, requirement, deficiency symptoms and their remedial measures etc. The advent of high-throughput sequencing technologies together with bioinformatic tools identified the presence of 100 trillion of microorganism belonging to all forms of life in the gastrointestinal tract. In fact, the whole gut microbial consortia are considered as a virtual organ in human and animals and is responsible for the development and functioning of series of essential physiological activities including digestion, absorption, metabolism, immunity, homeostasis, brain and gut development, resistance against disease etc. According to the modern scientific dogma, the human being is in fact a “Superorganism”, carrying consortium of numerous symbiotic Eukarya, Bacteria, Archaea and Viruses. The gut microflora has been co-evolved with human and presumed to maintain an intricate balance among different microbial communities. Upon introspection about the functionality of the gut microflora, it is amply clear that a few of these microorganisms have the ability to make healthy state; while other members are responsible for health-related problems including food poisoning, diarrhoea, abdominal pain, indigestion, inflammatory bowel disease, predisposition to colon cancer, compromising immunity, allergy etc.

Introduction

Food is any substance or a product obtained from nature, which contains nutritive and non-nutritive components that help to maintain or improve the health of an individual if consumed in optimal amount (Sukhneet and Malhotra, 2014). Globalization of food production and procurement makes food chains longer and more complex and increases the risk of food safety incidents. Infections and diseases arising from contaminated/adulterated food remain threats to global public health (Havelaar et al., 2015; Luo et al., 2019). The WHO reports shows that every year foodborne diseases cause the death of 33 million people including many children (WHO, 2019). Food safety is linked to the presence of foodborne hazards in food at the point of consumption. Since food safety hazards can occur at any stage in the food chain it is essential that adequate control be in place. Therefore, a combined effort of all parties in the food chain is required to deliver safe food products to the final consumer. Effective and harmonized food safety systems shall manage and ensure the safety and suitability of food in each link of the supply chain.

Although the food industry has the prime responsibility of ensuring safe foods in most societies, the maintenance of a safe food supply is regarded as a responsibility of the government of that country. This responsibility has, over many years, led governments to adopt legislation, standards and regulations for food processing and to impose penalties when a business fails to meet the expectation. As with any legislation, an effective system for detecting failures to comply with the legal requirements increases compliance and hence, in this case, promotes food safety. Governments have an obligation to protect consumer health and this includes protection against food borne diseases. Food standards are therefore necessary to aid governments in protecting public health. However, the standards can only be effective when they are enforced through an efficient food control system. This therefore requires comprehensive legislation inspection, food monitoring and food borne surveillance. An efficient food control system must be supported by competent staff with good laboratory services. Political and institutional support and stability are also important elements.

Abstract

In the present horticultural scenario of India, this is the burning issue as how best the underutilized horticultural crops can be exploited. There is tremendous potential for these crops especially for diversification. These plants can generate employment for millions especially to the natives ofdeveloping counties besides nutritional security. Traditional wisdom of Indian farmers if used properly can pave the path for transforming the economy of developing nations. There are great opportunities for the people to identify crops, explore their market, standardize their cultivation and processing techniques, document them and provide jobs to millions. This will salvage the people from exploitation by multinational companies of developed nations and can give a new direction to sustainable agriculture.

Due to the globalization of the food supply there has been a need to strengthen food safety systems in and between the countries. In this regard the World Health Organization (WHO) has promoted efforts in improving the food safety from farm to plate which started on World Health Day, 7 April 2015. The World Health Day 2015 slogan was “From farm to plate, make food safe”. The informal food production and marketing system is still strong in most countries of the South-East Asia Region, for the enforcement of food safety regulations. Traditionally, the preferences have been for hot and well-cooked food. These habits are partially responsible for preventing foodborne infections. In many countries street food has become very popular as it is cheap. Hygienic conditions are improving, provided that potable water supply and clean facilities are ensured by municipal authorities. Many waterborne and diarrhoeal diseases could be prevented with the introduction of bottled drinking water. The awareness and education on food safety by the consumers has strengthened in the enforcement of food standards. This has improved the hygienic practices and has helped in the prevention of foodborne illnesses. The “WHO Five keys to safer food” serves as the basis for educational programmes to train food handlers and educate the consumers. These help in preventing the foodborne illnesses. The important points are to keep food surfaces clean, washing of all utensils, plates and cutlery, keeping the raw food separate from the cooked food. Cooking food thoroughly to the appropriate temperature, keeping food at safe temperatures, both for serving and storage and using safe water and raw materials.

Saccharification: The process of breaking a complex carbohydrate (starch or cellulose) into its monosaccharide components is called saccharification.

Saccharimeter: A device (polarimeter) used for measuring the amount of sugar in a solution is called saccharimeter. It is also known as saccharometer. It is a hydrometer with a special scale.

Saccharin: A crystalline compound C7 H5 NO3 S that is unrelated to the carbohydrates, is several hundred times sweeter than sucrose, and is used as a calorie free sweetener.

Saccharomyces: It is one of the genus (Saccharomyces) of the family Saccharomycetaceae and is usually unicellular yeasts (brewer’s yeast) that are distinguished by their sparse or absent mycelium and reproduced asexually by budding.

Safe food: Food that provide maximum nutrition and quality while revealing a minimal hazard to public health and they assume any risk present to be minimal.

Today it has been understood that yeasts have great potentials as novel probiotics (Nayak, 2011). They have been found to be safe for human consumption in traditional fermented foods (Jakobsen and Narvhus 1996). Yeasts have a great physiological importance inside the gastrointestinal (GI) tract (Czerucka et al., 2007; Gatesoupe, 2007). Jespersen (2003) has mentioned the role of consuming fermented foods and the health benefits attributed with it.Yeasts are known for prevention of table olive (Arroyo lopez et al., 2012). Bonatsau et al. (2015) have shown multi-functional property of yeast as starters.

Packaging is an essential tool to make products stand out from the competition. Beyond the shelf appeal, packaging also provides protection to the contents from physical, chemical and microbiological hazards. However, a lesser known fact is that the same packaging material could also be a source of chemical contaminants, and hence, may impact food safety as well as food quality. Among the different components of packaging material, packaging inks play a critical role as they might hamper product and consumer safety.

Each packaging ink is a mixture of different chemicals and some of these chemicals might be harmful with the potential to migrate to the foodstuff. In terms of risk, the chemicals could be toxic, carcinogenic, mutagenic, repro-toxic and even endocrine disruptors. The chemical contaminants from the packaging inks can get transferred to the foodstuff by a process called migration.

Over the last two decades, a number of scientific papers have been published, revealing the migration potential of substances from the printed matter. However, the first big scandal that brought the subject alive was about the f indings of isopropyl thioxanthone (ITX, a low molecular photo initiator used in UV inks) in baby milk and other liquid foodstuffs all over Europe, resulting in a product recall in the year 2005. The food scandal alerted the packaging chain about the linkage between packaging inks and food safety. Consequently, the authorities issued strict regulations for the application of printing inks as well as general food control measures.

1. Introduction

Storage is an important part of any food grain processing chain. Storage of raw and processed food grain has been used by humans since time immemorial as a prerequisite for insuring food security due to off time availability, and for holding seed grain for long periods (Pande and Mishra, 2012). After harvesting grains may be required to be stored for long periods before these are marketed or used as food or seed. The grain storage is mainly carried out for three purposes viz (i) to retain a supply of food (ii) to service a trading system and (iii) to retain seed for planting the following season (Hall, 1980). Storage is, therefore, carried out by the producer, trader, processor, and the exporter and at all these levels the methods adopted affect the quality of food stuffs. The length of time grains can be safely stored will depend on the condition it was harvested and the type of storage facility being utilized. Grain storage and handling is a major concern for food grain growers and processers worldwide (Mohan et al., 2011; Pande and Mishra, 2012). Depending upon the condition and uses food grains may be stored in different storage structures using traditional or modern storage methods. Different storage structures are used by farmers with the traditional names like Gumma, Oliya and Jute bag. Caswell (1973) recorded 50 to 60% of insect damage on food grain after six months of traditional storage. A typical range of storage methods encountered in tropics and subtropics region is given in Table 1.

Introduction

Food is an important contributor to human well being and is essential for sustaining life. From this point of view food is expected to contains essential nutrients, such as carbohydrates, protein fat, vitamins and other essential minerals which support life. The food production initially started for individual sustenance which slowly developed in to a community requirement necessitating the involvement of man and machinery for various activities concerned with food. The progress from famine in many parts of the world to the level of self-sufficiency and the role of science and agriculture have been absolutely significant in many parts of the world. India is no exception and the green revolution during the post 1960s and the succeeding blue and white revolution ensured the self-sufficiency in India.

Of late food acts as a vehicle for inclusion of hazard in food meant for human consumption. According to Grunert (2005), factors responsible for the alarmingly higher rate of concern by public include significantly higher number of food scares in public domain, critical understanding of production and processing of certain food products sold in the market as well as the diversified food choices of the consumers in different parts of the world. Besides, the increased awareness of the consumers on food related health issues also have its share in the increased concern on food at large.

Presently food is considered as a commodity which crosses international border concurrent with the human movement and hence quality has become a major concern. The traditional food is getting replaced with value added products, particularly ready to cook, ready to eat, partially cooked products, etc. consequent to life style changes. In order to meet the demand of the trade, the conventional processing is getting replaced by community processing or industrial processing with increased number of players in the primary production, processing, transportation and other value chain activities prompting larger product control. In the process, consumers are exposed to a variety of products produced as business ventures in modern food production systems needing efficient quality control and quality assurance to tackle the issues of quality. The increased incidence of hazards in both raw materials and final products consequent to the absence or inappropriate production schedule calls for increased food safety concerns. Besides the shelf-life of the products, addition of chemicals during the production also add to the concern.

Introduction

As the world population continues to grow, the global market for food products is expanding, together with an increased emphasis on food safety. Consumers have become more discerning in the type of foods they buy and how these foods affect their health and well being. Food safety is generally defined as the assurance that the food will not cause any harm to the consumer when it is prepared and/or eaten according to its intended use ( To achieve food safety, a management system has been developed which aims on preventing problems before they occur, rather than trying to detect failures through end product testing. It also places more responsibility for ensuring food safety on food manufacturers, who have to develop control and traceability of their products from ‘farm to plate’. The system requires the identification of specific hazards throughout the entire process of food production, concentrates on the points in the process that are critical to the safety of the product, and highlights measures for their control (Henson and Caswell, 1999).

Introduction

Tropical tuber crops viz., cassava (Manihot esculenta Crantz), sweet potato (Ipomoea batatas), taro (Colocasia esculenta), yams (Dioscorea sp.), Elephant foot yam (Amorphophallus paeoniifolius), arrowroot (Maranta arundinacea) etc., are the important sources of starch after cereals and are used as staple or supplementary food in many of the tropical and sub tropical countries. Being the crops with adaptability to wide range of soil, climate and environment of the tropics and sub tropics and requiring minimum agronomic input and care for growth, they can be very well fitted into the prevailing cropping systems. They are the most efficient converters of solar energy and have the ability to reserve energy in the form of carbohydrate in the bulky roots and hence tuber crops are also called as calorie density crops. Tuber crops, though branded as poor man’s crops in rural areas have considerable unrealized potential for processing into high end products for food, feed and industrial uses. They are generally consumed as vegetable after boiling it in water and eaten along with some spicy curry or by extracting starch or flour. Besides serving as an insurance crop during times of food scarcity, it can also function as an industrial raw material for a wide spectrum of products

Introduction

Baking is a traditional activity which has a long history, and according to some recent discovery, it is indicated to be dated back to as early as around 21,000 B.C. It is termed as a complex operation of prolonged heat transfer through radiation, convection, and conduction. Baking is considered to be one of the oldest methods of cooking, most commonly the bread baking but it is not only limited to the bread instead it provides a wide range of products such as cookies, pastry, pizzas, breakfast cereals, etc. Baking includes lots of raw materials such as flour, water, yeast, milk and milk derivatives, shortening, sugars, eggs and some minor ingredients such as malt products, cocoa products, dough improvers, inhibitors of microbiological spoilers, spices, etc. Food items which are rich in sugar and carbohydrates such as chocolates, candies are known as confections, and the art of making these products is termed as confectionary. Confectionery can be broadly classified into two major categories, namely sweet confectionary and baked confectionery. Conventionally cake was defined as the flour based sweet food, whereas bread was defined as the flou-based non-sweet food.

Baked confectionery, also known as flour confectionery includes products such as cakes, pastries, and similar baked goods, especially which are served in dessert. These delicacies hold history not much later to the bread baking. Earlier the baked confectionery especially cakes were only affordable by the rich and were prepared for special occasions such as festivity, celebrations, etc. but now a large number of flour confectioneries are being produced in the world which is delightfully acceptable and are much more in demand by a various group of people.

Wheat Production and Consumption

Wheat, rice, maize, barley, sorghum and the millets are the main crops that provide nutrition to people worldwide. The production of grains in different countries depends on the various factors such as water availability, soil conditions, temperature, cultural and economical are most critical factors. Nutritional needs of the developing countries are more dependent on cereal grains compared to the developed ones. Approximately 60% of the calorie requirements in the developing countries are met from cereals. The International Grains Council (IGC) forecasted for total grains production in 2017-18 to 2.079 billion tonnes. There is a need to have major research to increase their productivity, reduce storage losses, enhance efficiency of processing and to ensure food safety in the entire supply chain. Among them, wheat is one of the most important crop species and is a staple food for about 35% of the world population. Wheat is unique among the grains because of its protein type, which when mixed with the water by mechanical forces yields into viscoelastic dough due to the formation of gluten. This unique property of dough is capable of retaining the gases which are produced by yeast or baking powder resulting in the production of an array of leavened products such as bread, cookie and cake.

Wheat is also an important source of dietary proteins for humans but like other cereals, it is deficient in the essential amino acid lysine. Wheat crop is adaptable to the wide range of growth conditions compared to other major cereals and hence it is cultivated widely throughout the world. Wheat cereal is the major dominating staple in the North Africa, Central and West Asia, providing the 50% of the calorie requirement in the region. The world wheat production is 740 million tonnes, with China ranking first by producing 125 million tonnes of wheat annually. Other major wheat producing countries are India, USA, Russia Australia, France and Canada. The global per capita consumption of wheat is about 66 kg, which varies widely in different regions. In Algeria, Egypt, Israel and other Middle East as well as Eastern Europe the consumption of wheat flour is high (150 kg/year). The food uses of wheat in developing countries are more than 80%, while in developed countries it is less than 50%. The consumption of the wheat in developing countries is significantly increasing due to globalization and growing economies. After rice, wheat is a major cereal crop in India and contributes to more than 50% of the total calories. It is grown in the cooler regions of India during the period of November to April. In grain production, rice ranks first with the production of about 105 million tonnes per year. In India, total grain production is 136 million tonnes for the year 2016-17 as per the International Grains Council. India ranks second in the production of wheat in the world and produce about 95 million tonnes annually. The bread wheat (astevum) contributes to approximately 94% while durum wheat is 4% and dicoccum wheat accounts to 2%.

Introduction

Oils and fats are the major class of compounds in lipids. Oils and fats are essential nutrients in both human and animal diets and produced from plant, animal, marine and microbial sources. Dietary oils and fats are concentrated sources of metabolizable energy (9 kCal/gram) compared to protein or carbohydrates (4 kCal/g). According to the recommendations of Food and Agriculture Organization (FAO) of the United Nations, the maximum total fat intake for adults is 30 to 35% E (total energy calories). The minimum fat intake recommended is 15% E for most individuals and 20% E for women of reproductive age and for malnourished populations (FAO, 2010).The current dietary guidelines for optimal health and prevention of diet-related chronic diseases like diabetes, coronary heart disease (CHD), cancer and obesity emphasize the importance of both the quantity and quality of total fat in the diet. In addition to the caloric and nutritional value, oils and fats exhibit many functional properties in the diet. Oils and fats enhance the value of foods by releasing the flavours of other food components, provide texture, increase palatability of food, and impart the feeling of satiety after eating. Oils and fats provide essential fatty acids (omega 3 and 6) which are the building blocks for several metabolic pathways for the healthy life. Oils and fats are excellent carriers of fat-soluble vitamins A, D, E and K, and the absorption of these vitamins is impaired at very low-fat intakes. Due to this reason, sseveral countries have started using edible oils and fats as a suitable vehicle for the fortification of vitamin A and D as edible oils are consumed by almost everyone (Diosady and Krishnaswamy, 2018). Oils and fats are structural bodily components, and are involved in vital physiological processes, including growth, development, inflammation, and brain function. Lipoproteins are important cellular constituents, occurring both in the cell membrane and mitochondria, and also serving as the means of transporting lipids in the blood. Oils and fats are responsible for making biological membranes such as phospholipids and cholesterol, vital for making the cell membranes. Most of the edible oils contain several minor constituents which are known as potent nutraceutical constituents with several health benefits. In addition to edible purpose, oils and fats are being used in many industrial applications such as soaps, detergents, cosmetics, lubricants, specialty chemicals, biofuels, etc. Hence oils and fats are indispensable source for food (edible oils and nutraceuticals), fuel (biodiesel, lubricants etc.) and fabulous chemicals (oleochemicals).

Introduction

Fruits and vegetables (F&V) are good sources of phytochemicals like carotenoids, phenolics, dietary fibers, minerals and vitamins and are considered as important components of a healthy diet. These bioactive compounds in fresh F&V have been linked to delay in onset of many diseases including cardiovascular, chronic and other non-communicable diseases. WHO reported an approximate 3.9 million deaths all over the world in 2017 due to insufficient consumption of F&V (WHO, 2019). Hence, health advisors and nutritionists recommend inclusion of fresh fruits and vegetables to complement the daily diet, and WHO recommends the consumption of a minimum of 400g F&V daily in five portions. However, beneficial raw F&V may contain pathogenic microorganisms, such as Salmonella, E. coli, and Listeria that can pose threat to consumer safety. Since fruits and some of the vegetables are eaten raw, there are chances that pathogenic microorganisms may also enter food chain. Nearly a half of food borne illnesses in United States are caused by microbes on fresh produce (CDC, 2019). The F&V are handled by producers, dealers, wholesalers, retailers and several intermediaries, and therefore, are at greater risk of possible contamination till it reaches the consumer.

The ever increasing demand for F&V has led to overexploitation of pesticides usage in the farms, leading to harmful pesticide residues in air, soil, water and on the F&V surfaces. Thus, it is imperative to maintain the quality and safety of F&V all through the supply chain. In this chapter, we discuss various pre and postharvest factors affecting the quality and safety of F&V, storage techniques to extend shelf life and reduce the safety threat as well as methods available for microbial and pesticide decontamination of fresh F&V.

Introduction

The conventional thermal processing technologies are dependent on high temperature to control the growth of pathogenic and spoilage causing microorganisms. However, the exposure to high temperature for prolonged time results in several biological, chemical and physical modifications leading to their sensory, nutritional and textural changes. Currently, consumers are more interested in minimally processed food products with improved safety and shelf life. In order to fulfill the market demand, innovative non-thermal food processing techniques to inactivate the microorganisms have been developed. These techniques retain freshness, nutritional value and sensory characteristics of food items without any significant thermal degradation.

Non-thermal technologies are used in interdisciplinary sciences, and in many other applied areas. The three main pillars related to the use of novel food technologies are food safety, quality and environmental impacts. Besides the food safety and quality dimensions, these non-thermal processing technologies have the possibility to shorten treatment time, lower energy consumption, and lower carbon footprint. Depending on the source of energy transfer, non-thermal technologies have different types of action. It includes inactivation of microorganisms in radical formation (plasma, ultrasound, ozonation, UV light, etc.); mechanical action through hydrodynamic effects, shock waves (ultrasound and plasma), electric and magnetic fields; or extremely high pressures that are causing disruption of the cell organelle.

The novel non-thermal technologies destroy the pathogens at room temperature as well as utilize less energy compared to conventional ones and have the potential of developing new food products having distinctive functionality. For optimizing the product quality, time for processing and also to inactivate the enzymes and bacteria, each of these techniques can be used either alone or in combination. The adverse effect of some technologies on the sensory, texture, and nutritional values of food could be overcome by using hurdle technology. Combination strategies are more efficient for improving the food quality and have the potential to ensure the microbial safety of foods. An overview of the effect of novel process technologies on the quality and safety of food is elaborated in this chapter.

The dairy processing industry in India deals mainly with the fluid milk and/or short shelf life products, despite India being the largest producer of milk in the world, with an output bordering the 110 million ton mark. This feature offers immense potential in the area of R&D in dairy processing in terms of value addition. The potential gains are many, including higher returns for producers and advantages in terms of health, therapeutic, nutritional and/or gastronomy for the consumers. However, the Indian Dairy Industry can amass possible benefits by being a global contender in trade only when its products are at par with those available in the rest of the world in terms of price and quality. It is evident from the post-WTO scenario that for sustainability, Indian dairy industry will need to maintain wholehearted commitment to quality management right from the farm to the consumer, shedding its inhibitions and resistance to changes.

9.1. WTO Agreements and Food Industry

The General Agreement on Tariffs and Trade (GATT) was formed in 1947 and lasted until 1994, when it was replaced by the World Trade Organization (WTO) in 1995. The WTO is the only global international organization dealing with the rules of trade between nations. It provides a forum for negotiating agreements aimed at reducing obstacles to international trade and ensuring fair deal for all, thus contributing to economic growth and development. Currently, there are 16 agreements to which all WTO members are parties and two agreements to which only some WTO members are parties. WTO currently has 153 members, of which 117 are developing countries or separate customs territories. The Head Quarters of WTO is at Geneva (Switzerland) and the official languages are English, French and Spanish.

Introduction

The dairy sector is one of the most important food sectors, which introduced the physical, chemical, microbiological and sensorial criteria duringprocessing steps and in the final dairy products. Several agents/ingredients of non-dairy origin are commonly used to maintain the quality of milk and milk products during processing, transportation and storage. In general, such ingredients are known as food additives and it can be antioxidants, coloring agents, flavoring compounds, preservatives(naturals and synthetic),artificialsweeteners, vitamins, minerals, stabilizers, emulsifiers and curing agents. From the safety point of view, some toxic agents can come into contact of dairy products duringmilking, storage or processing; such as fungal toxins, heavy metals, antibiotics or radionuclide’s, etc., which need to be identified and processed accordingly. Although addition of food additives in food/dairy product is controlled under country specific food laws or Codex Alimentarius Commission (CAC), few dairyproducts such as butter and unflavored fermented milk products are highly restricted for food additives.

Introduction

Packaging helps to maintain the benefits of food processing and enables safe transportation of foods from their point of origin to point of consumption without compromising the wholesomeness of processed food in terms of its physico chemical and microbiological quality. However, packaging technology must balance protection effects with environmental issues, material and energy costs and should comply regulations on municipal solid waste disposal and release of pollutants. These regulations are different in jurisdictions around the world.

Safety implications of food packaging raise concern in recent times due to advances in technology leading to variety of packaging materials and additives. Also there is an alarming situation of damages to environment and natural resources of the planet, due to indiscriminate use and disposal of packaging materials.

However, food packaging as a means to protect and preserve food integrity, to enhance its shelf life and improve consumer appeal cannot be overlooked.The applications of food packaging and its primary role to protect and preserve food has remained the central focus of packaging innovations.Packaging materials are available in variety of forms and are made up of variety of ingredients.While paper. While paper, plastic, petroleum based materials have been in use for many years, the recent approach is towards environment friendly options from agricultural produce such as plant fibres sugars and starches. Advances in packaging has led to development of newer materials and combinations with specific economic and functional benefits.Food packaging ,in general , provides the ability to withstand thermal processing and acts as a barrier against light, moisture,oxygen and other sources of contamination.

Introduction

Food packaging materials development has been driven by the need to protect food products throughout distribution and storage. Nanotechnology offers opportunities to improve technical packaging features and develop sustainable smart packaging and sensors for monitoring product quality over time. However, the inherent permeability of conventional packaging materials to atmospheric gases and water vapour poses challenges in maintaining optimal conditions for food. The integration of nanotechnology into food packaging has emerged as a promising solution to address these challenges. Nanocomposite materials, composed of a polymer matrix and nanoscale fillers, offer a novel approach to enhancing packaging performance while addressing issues related to food spoilage, degradation, and safety.

Nanocomposite materials encompass a range of nanomaterials, including inorganic (i.e., metals and metal oxides), nanoclay, organic nanomaterials, and nanoemulsions (Huang et al., 2018; Jamaleddin et al., 2019; P. Kumar et al., 2020). NMs are used for three types of packaging: improved packaging, active packaging and intelligent packaging, Nanotechnology facilitates the development of improved bio-based packaging, enhancing mechanical strength and barrier performance while maintaining stability under varying environmental conditions (S. Kumar et al., 2020; Zhang et al., 2020). Active packaging harnesses nanostructures to encapsulate bioactive compounds, encompassing antimicrobials, antioxidants, and oxygen scavengers, which can interact with food and extend its shelf life (Motelica et al., 2020). Intelligent packaging releases active compounds in response to environmental triggers such as temperature or pH changes, transforming packaging into an intelligent, actively responsive material (Jamróz et al., 2019).

For a researcher, laboratory is another home where he/she spends much time every day. As we take care of cleanliness, safety, and design of our home, we must take care on these aspects of laboratory too. It is the responsibility of the laboratory personnel to ensure cleanliness/safety of the laboratory. Since every laboratory chemical and equipment have the potential to cause harms if not used with appropriate precautions. Hence, as a laboratory user, it is our responsibility to follow the basic safety/biosafety rules and regulations set up by the Principal Investigator of the laboratory, Institutional Biosafety Committee, as well as by other regulatory authorities, if any. We must be aware of the general/personal protective measures, cleanliness and laboratory waste disposal prior to joining a research laboratory. In this chapter we will briefly discuss about personal safety, chemical safety, cleanliness, waste disposal, etc.

A. Personal Safety

1. Personal Protective Equipment (PPE)

Personal protective equipment (PPE) is special arrangement to protect the worker from specific hazards because of using a hazardous substance. PPE does not reduce or eliminate the hazard, but protects the wearer from exposure to hazardous material. PPE should not be reused or brought outside the laboratory as these might increase the risk of spread hazardous material or contamination. Used PPE must be kept inside the laboratory in a designated waste storage area adjacent to the work area.

Josephson (1977) studied the effects of food irradiation and sterilization. In radiation sterilization of food (radappertization) the food is exposed in sealed containers to ionizing radiation at absorbed doses which are high enough (25-70 kGy) to kill all organisms of food spoilage and public health significance. Radappertization is analogous to thermal canning for enhancing the shelf stability. In dry products the radappertization process requires the food to be heated to an internal temperature of 70-80°C (bacon to 53°C) to inactivate autolytic enzymes which catalyze spoilage during storage without refrigeration. In order to reduce off-flavors and odors, undesirable color changes, and textural and nutritional losses from exposure to the high doses of irradiation the foods are vacuum sealed and irradiated frozen. Radappertozed foods have the characteristic of fresh foods prepared for eating. Irradiation can be a good substitute for some chemical food additives such as ethylene oxide and nitrites which are toxic, carcinogenic, mutagenic or teratogenic either wholly or in part. Till now, there is no evidence of any adverse effects of radappertization on the foods. Extensive research has taken place towards the space food. During the planning of Apollo missions, Space Food NASA Technical Reports Server (Space Food NASA Technical Reports Server, NTRS ,1994 ) conducted trials including freeze drying.

The use of salt (Sodium Chloride) in food products is an important method of preservation. It not only imparts taste to the products but also helps in checking spoilage of fruit products. Salt is added to pickles, sauces, chutneys, ketchup, and many other products. Food Safety & Standards Regulation has specified the maximum limit of salt in different products. Therefore, the estimation of salt content in food products is considered as an important attribute in food quality control.
Direct Titration Method
Direct titration (Mohr’s) method is used for the determination of salt content in food products as a routine quality control. In this method, the sample is titrated against a standard solution of silver nitrate for the estimation of salt.
Reagents
0.1 N NaOH, Phenolphthalein 1% indicator solution
0.1N Silver nitrate (AgNO3)
5% Potassium Chromate

8.1 Introduction

Electron Microscopes were developed due to the limitations of Light Microscopes which are limited by the physics of light to 500x or 1000x magnification and a resolution of 0.2 micrometers. In the early 1930’s this theoretical limit had been reached and there was a scientific desire to see the fine details of the interior structures of organic cells (nucleus, mitochondria etc.). This required 10,000x plus magnification which was just not possible using Light Microscopes. The Transmission Electron Microscope (TEM) was the first type of Electron Microscope to be developed and is patterned exactly on the Light Transmission Microscope except that a focused beam of electrons is used instead of light to see through the specimen. It was developed by Max Knoll and Ernst Ruska in Germany in 1931. The first Scanning Electron Microscope (SEM) debuted in 1942 with the first commercial instruments around 1965. Its late development was due to the electronics involved in scanning the beam of electrons across the sample. Electron microscope has been a valuable tool in the development of scientific theory and it contributed greatly to biology, medicine and material sciences. This wide spread use of electron microscopes is based on the fact that they permit the observation and characterization of materials on a nanometer (nm) to micrometer (µm) scale. Electron Microscopes are scientific instruments that use a beam of highly energetic electrons to examine objects on a very fine scale. This examination can yield information about the topography (surface features of an object), morphology (shape and size of the particles making up the object), composition (the elements and compounds that the object is composed of and the relative amounts of them) and crystallographic information (how the atoms are arranged in the object). A scanning electron microscope (SEM) is a type of electron microscope that produces images of a sample by scanning it with a focused beam of electrons. The electrons interact with atoms in the sample, producing various signals that can be detected and that contain information about the sample surface topography and composition. SEM can achieve resolution better than 1 nanometer. The combination of higher magnification, larger depth of focus, greater resolution, and ease of sample observation makes the SEM one of the most heavily used instruments in research areas today.

1.1 INTRODUCTION

India is the seventh largest country in the world in total geographical area and has second largest population next to China. Around 55 to 60 per cent of its population depend on agriculture and allied activities. Horticultural crops constitute a significant component of total agricultural production in the country. They require very intensive care in planting, carrying out different cultural operations, manipulating growth, harvesting, packing, transportation, marketing, storage and processing.

1.2 AGRICULTURE

The term agriculture broadly refers to the technology of raising plants and animals. Agriculture is the science, art or practice of cultivating soil, producing crops and raising livestock in varying degree of proportion followed by marketing. In other words, it is the activity of man to produce three basic needs of human being, i.e., food, fuel and fibre (3F) by using terrestrial resources optimally. The term agriculture is derived from two Latin words ager meaning field or land and cultura meaning cultivation or growing.

1. Introduction

Fruits and vegetables represent highly perishable items of agriculture, mainly because of their high water content in fresh form. Their production, yield and management under agriculture is dealt essentially under horticulture. They abound in species and varieties in different geographic regions of the world, influenced by the agro-climatic conditions. Some are endemic and confined to certain geographic regions only. Accordingly, they may be tropical, sub-tropical or temperate, depending on their requirements of temperature for growth and yield.

The term food processing covers an enormous field from simple boiling to the use of irradiation to predict the consequences on the nutritional value of fruits and vegetables. Generally, the food processing methods can be categorized as in intentional processing method (milling, peeling, washing trimming etc.), inevitable processing method (blanching, sterilizing, cooking, shredding, and drying) and accidental (inept processing or system). The nutritional losses associated with fruits and vegetable processing with different processing techniques and conditions employed. For example, the heavily processed products are although safe for human consumption but allied with reduced nutritive value. In this regard access to safe and nutritious food is considered as a basic right of humans as it is one of the ways to prevent various ailments by building better health and enforcing immunity. Escalating help awareness has guided the processes to opt for minimal processing food in recent years.
Food commodities subjected to minimal processing are capturing the market Suring to widespread concept that they might help in meeting the current requirement of vital nutrients. Development of minimally processed commodities especially fruits and vegetables have augmented at both retail and consumer levels during last years. Various organisations WHO, FAO, USDA boost up the utilization of minimally processed perishable commodities including vegetables and fruits to lessen the risk of cardiovascular diseases and cancer. Changes occurring in the eating habits have been quoted due to high anxiety for on time availability of these minimally processed fruits and vegetables. Sales of fresh cut fruits and vegetables continue to burgeon and food industries are persistently expanding primary because of consumers’ trend of health realization.

Introduction Sea buckthorn berry belongs to Elaeagnaceae having 6 species (Hippophae rhamnoides, Hippophae neurocarpa, Hippophae salicifolia, Hippophae gonicarpa, Hippophae tibetiana and Hippophae litangenis) and 12 subspecies grown all over the world. India, is having its plantations in chilly and barren conditions of Ladakh, Himachal Pradesh, Uttarakhand, Sikkim, and Arunachal Pradesh. Berries and fruits are believed to have such bio-active compounds like antioxidants in them that it has constructive consequences on health of human beings. Moreover, they are been proven in lessening the cardiovascular diseases, age related issues,cancer etc. Berries of Sea buckthorn is an abundant provenance of these bio-active compounds. One of the major lipophilic compounds is carotenoid pigments. And the concentration of the same has difference depending upon origin of plant, harvesting period and weather conditions. Nutritionally, the fruits have a substantial amount of carbohydrate, protein, organic acids, amino acids, and vitamins. Moreover, it also contains bioactive compounds like carotenoids, dietary minerals, antioxidants, flavonoids, vitamin C, minerals, monosaccharide, organic acids, tannins, etc. Sea buckthorn plant parts are becoming one of the useful and promising nutraceuticals for the food industry and all over the world.

Introduction

Seafood provides about 25% of the global intake of animal protein and fetches a huge economy for developing nations. Many challenges concerning food safety and quality exist due to globalization and liberalization of the global f ish trade. International food safety regulatory bodies such as WTO and CAC through SPS and TBT Agreements work on ensuring food safety and quality. Adoption of risk-based analysis and implementation of the HACCP system are encouraged to maintain high seafood safety standards. International regulatory bodies set maximum residual limits for various environmental pollutants and contaminants to ensure consumer safety. Consumer education programs play important role to enhance awareness and improve transparency in the seafood supply chain. Internationally traded seafood products therefore need to meet the standards prescribed for food safety and quality requirements. In case of non-conformance, they could be detained, rejected and destroyed.

The global demand on seafood quality and safety requirement and the complexities of seafood supply chain make difficulty to ensure authenticity and traceability of seaood products in the market. Most seafood is exported from the country where it is caught while part of it via. a third country. Seafood pass-through complex supply chains involving, capture/culture, transshipment, processing, packaging, wholesale, retailing, and consumer. The global trade of seafood has led to a significant concern associated with seafood fraud.

12.1. Seafood Fraud

Seafood fraud is the practice of providing misleading information to the consumers about their product for financial gain. There are nine types of seafood fraud, which include species substitution, fishery substitution, IUU substitution, species adulteration, chain of custody abuse, catch method fraud, undeclared product extension, modern day slavery, and animal welfare

Introduction
 
Whilst food has long been used to improve health, our knowledge of the relationship between food components and health is now being used to improve food. Although most foods can be considered “functional”, in the context of this review, the term is reserved for foods and food components that have been demonstrated to provide specific health benefits beyond basic nutrition. Widespread interest in select foods that might promote health has resulted in the use of the term “functional foods”. Seaweeds are known for their richness in polysaccharides, minerals and certain vitamins [1], but they also contain bioactive substances like polysaccharides, proteins, lipids and polyphenols, with antibacterial, antiviral and antifungal, as well as many such other properties [2]. This gives seaweed great potential as a supplement in functional food or for the extraction of compounds. Physiologically active substances in marine algae are classified into two types based on the difference in the mechanisms viz. (i) non-absorbed high-molecular materials like dietary fibres, and (ii) low molecular materials, which are absorbed and which affect the maintenance of human homeostasis directly. Seaweeds are among the first marine organisms chemically analyzed, with more than 3,600 articles published describing 3,300 secondary metabolites from marine plants and algae, and they still remain an almost endless source of new bioactive compounds [3].

Plant secondary metabolism produces products that aid in the growth and development of plants but are not required for the plant to survive. Secondary metabolism facilitates the primary metabolism in plants. This primary metabolism consists of chemical reactions that allow the plant to live. In order for the plants to stay healthy, secondary metabolism plays a pinnacle role in keeping all the of plants’systems working properly. A common role of secondary metabolites in plants is defense mechanisms. They are used to fight off herbivores, pests, and pathogens. Although researchers know that this trait is common in many plants it is still difficult to determine the precise role each secondary metabolite. Secondary metabolites are used in anti-feeding activity, toxicity or acting as precursors to physical defense systems.

Concept of Delinting

Cotton seed clumps because of linters getting trapped on them after ginning. Gin-run seeds have very low flowability and do not singulate, making cleaning, upgrading, and precise metering in planting operations challenging or impossible. To singulate the seed and increase flowability, a variety of techniques have been and are employed. Most of the techniques entail removing the tags and linters entirely or partially, although other coating techniques have also been attempted with mixed results, both financially and technically (Mezynski, 1966; Webber and Boykin, 1907).

Methods of Delinting

1. Mechanical Delinting

Mechanical delinting is the usual technique used to improve the flowability of cotton seed. As previously indicated, mechanical delinting basically involves resharpening with finer, closer-spaced saws to remove part of the commercially valuable linters.

During processing seeds are cleaned, dried, and graded which are then treated with specific fungicide and insecticide. Seed at this stage is at best quality, which are then packaged into various packaging materials in a specific weight. Packaging or bagging of processed and treated seed is a requisite for easy handling of the bulk seed. Seed packaging can be defined as filling with seed, weighing and sealing the container whereas seed package as storage container where the seeds are kept for marketing or shipping or till the next sowing time.

The Packaging of Seed Involves

1. Filling bags/ containers of uniform size with seeds of known weight.

2. Providing leaflets inside the package with information about any specific cultural practices needed.

3. Attaching labels or certification tags having information regarding all the quality parameters.

4. Temper-proof sealing of the bag.

5. Storage or transportation of the bags.

Sanitation

Sanitation and maintaining good hygiene conditions are the important aspect of seed storage in storage godowns. Maintenance of good hygiene conditions will maintain the quality of produce in the long run (Mahapatra et al., 2014) and these practices should not be overlooked. The threshing floors should be cleaned properly before the operation so that there is no mixing of seeds or any insect-pests or pathogens. Harvesting and threshing should be followed by cleanliness of godowns to protect the seeds from the harmful pest and pathogens (Upadhyay and Ahmad, 2011). The storage godowns should be well lighted and the area should be preferably sunny, free from weeds and mostly situated at elevated areas. Damp areas should not be selected for building storage godowns as it will affect the moisture per cent of seeds thereby enabling easy growth of insect-pests and diseases (Parimala et al., 2013). Before bringing the new produce to the storage godowns, they should be cleaned properly. The cracks and crevices of storage godowns if any should be sealed ahead and the walls of the godowns should be whitewashed (Hazam and Kumar, 2022). The new seed lots should not be mixed with the older lots. The floors should be disinfected, and it should be cleaned regularly.

Concept: The preservation of viable seeds from the moment of collecting until they are needed for sowing is known as storage. Every year, a significant quantity of seeds loses their usefulness due to incorrect storage. Seeds need to be preserved and stored dry. As was previously mentioned, seed moisture content and storage temperature have the biggest effects on how long seeds last. The three main environmental elements that impact seed deterioration and loss of viability are moisture, temperature, and oxygen percentage. For over 90% of species, reducing seed moisture content (MC) to certain thresholds consistently lengthens the seed’s lifespan (Roberts 1973). These species are categorized as “orthodox” in terms of the conditions in which they should store seeds; under suitable dry, cool conditions, they typically maintain viability and germinability even after extended periods of storage. Ellis and Roberts’ improved viability equations have addressed the quantitative impacts of both drying and cooling for orthodox seeds. The life of the seed is often doubled for every 1% drop in seed moisture (when seed MC fluctuates between 5 and 14%) and every 5 oC drop in storage temperature (between 0 oC and 50 oC) (Harrington 1972). Low seed MC and low temperature are hence fundamentals of traditional seed storage. Careful regulation of these two factors is necessary to achieve optimal storage life. But most seed produced annually just has to be kept from harvest until the following planting season. Depending on the type of seed, it may or may not need to be stored at temperatures and relative humidity above room temperature. Regardless of any modifications made for unique uses, the local climate storage facility needs to have a few essential components (Barre, 1954; Wheeler and Hill, 1957).