eChapters

ANALYSIS OF VARIANCE

Analysis of Variance (ANOVA) is a hypothesis-testing technique used to test the equality of two or more population (or treatment) means by examining the variances of samples that are taken. ANOVA allows one to determine whether the differences between the samples are simply due to random error (sampling errors) or whether there are systematic treatment effects that cause the mean in one group to differ from the mean in another.

Most of the time ANOVA is used to compare the equality of three or more means, however when the means from two samples are compared using ANOVA it is equivalent to using a t-test to compare the means of independent samples.

ANOVA is based on comparing the variance (or variation) between the data samples to variation within each particular sample. If the between variation is much larger than the within variation, the means of different samples will not be equal. If the between and within variations are approximately the same size, then there will be no significant difference between sample means.

The two-way ANOVA compares the mean differences between groups that have been split on two independent variables (called factors). The primary purpose of a two-way ANOVA is to understand if there is an interaction be- tween the two independent variables on the dependent variable. For example, you could use a two-way ANOVA to understand whether there is an interac- tion between gender and educational level on test anxiety amongst university students, where gender (males/females) and education level (undergraduate/ postgraduate) are your independent variables, and test anxiety is your depen- dent variable. Alternately, you may want to determine whether there is an in- teraction between physical activity level and gender on blood cholesterol con- centration in children, where physical activity (low/moderate/high) and gender (male/female) are your independent variables, and cholesterol concentration is your dependent variable.

The interaction term in a two-way ANOVA informs you whether the effect of one of your independent variables on the dependent variable is the same for all values of your other independent variable (and vice versa). For example, is the effect of gender (male/female) on test anxiety influenced by educational level (undergraduate/postgraduate)? Additionally, if a statistically significant interaction is found, you need to determine whether there are any “simple main effects”, and if there are, what these effects are (we discuss this later in our guide).

Veterinary oncology is an upcoming field in recent years due to the integration of the importance of spontaneous canine cancers in understanding and developing novel strategies for human cancer management. We are stepping towards a very exciting era in veterinary oncology, where comparative oncology has played a significant role for many decades in understanding the molecular basis of cancer in companion animals. The comparative animal models have generated remarkable interest in both academia and industry. Studies have demonstrated the utility of comparative oncology in understanding drug resistance and tumor biology, contributing to the translational research[1]. This has yielded breakthrough discoveries, from advanced diagnostics to novel therapies, providing opportunities to improve the longevity and quality of life of the pets. These inputs have led to advancements such as the first conditionally approved chemotherapeutic agent for lymphoma, novel BRAF testing for canine urothelial carcinoma, tyrosine kinase inhibitors for mast cell tumors, updates in stereotactic/FLASH radiation, and novel immunotherapeutics. As the understanding of cancer biology has advanced, the field of veterinary oncology has grown significantly, paralleling developments in human oncology. Veterinary oncologists treat a variety of cancers in domestic animals, particularly dogs and cats, but also in exotic animals, horses, and even zoo animals. While the types of cancers in animals share similarities with human cancers, treatment strategies can differ significantly due to species-specific differences in anatomy, physiology, and response to therapies [2, 3].

Punjab, known as the “breadbasket of India,” relies heavily on livestock, particularly cattle and buffalo, for its agricultural prosperity. These animals play a crucial role in the region’s dairy industry, contributing significantly to milk production and rural livelihoods. However, like all livestock, cattle and buffalo in Punjab are susceptible to a range of diseases that can impact their health, productivity, and overall well-being. Timely and accurate diagnosis is essential for effective treatment and prevention. This article explores some of the most common diseases affecting cattle and buffalo in Punjab, along with the diagnostic procedures used to detect and manage these health issues, ensuring the sustainability and growth of the dairy sector.

Hydatidosis

Hydatidosis is a zoonotic disease caused by metacestode stages of parasite Echinococcus granulosus and is recognized as a major public health problem worldwide. This tapeworm is found in small intestine of the canid (definitive host) where it produce proglottids containing eggs. Upon shedding through faeces the later are ingested by the cattle, buffaloes and other intermediate hosts including man. These hatch in their intestine and the oncosphere invade circulatory system and lodge in various organs (liver, lung, brain etc.) where they develop into large thick walled unilocular cavity called hydatid cyst, which expands and can reach very large size and buds hundreds and thousands of protoscoleces endogenously. In a survey on hydatid cysts, a prevalence rate of around 5% has been recorded in more than 4000 meat samples of different animals in Punjab.

The Central Nervous System not only consists of the Brain but also involves the Spinal cord.

The spinal cord is responsible for conveying information from the Brain to the rest of the body via the peripheral nervous system. The spinal cord can be divided into 4 functional segments.

1. C1-C5 (Upper Cervical Segments)

2. C6-T2 (Brachial Plexus)

3. T3-L3 (Thoraco - Lumbar)

4. L4-S3 (Lumbo-Sacral Plexus )

It is important to note that the motor and sensory function of the pelvic limbs are not only controlled by the Lumbo-Sacral plexus but also involve the Thoraco Lumbar segments (T3-L3) of the spinal cord. It is important to understand not only the neuroanatomy, but also the functional anatomy before approaching a neurological patient.

Cardiomyopathies in acts can be classified broadly as Hypertrophic cardiomyopathy and non-hypertrophic cardiomyopathies. Usually, no alarming clinical signs are detected during routine examination in the preclinical stage except cardiac murmurs in some cats with HCM. This is because, that the cats are sedentary and excellent in hiding the signs of illness from the pet parents. Hence the onset is often acute with the sudden appearance of the clinical signs linked to congestive heart failure like severe dyspnoea, cardiogenic shock, aortic thromboembolism, arrhythmias and even syncope and death

Cats with CHF will be presented with severe dyspnoea, due to fulminant pulmonary oedema and or pleural effusion. The pleural effusion associated with CHF is usually a modified transudate, some cases it may be chylous .In echocardiography ,the size of the left atrium should be determined .If the LA size is normal ,then the dyspnoea may not be of cardiac origin .The presence of B-lines in the lung area in association with LA enlargement confirms cardiogenic pulmonary oedema. Stress, anaesthesia, I/V fluids and steroids leads to decompensation and fulminant pulmonary oedema. Thoracocentesis is will be helpful if there is dyspnoea due to significant pleural effusion. At least 300ml of effusion can be usually removed. The dyspnoeic cat is very fragile and should be handled carefully. Butorphanol may be given for anxiolytic effect. Oxygen administration is beneficial. Furosemide when given intravenously has an initial venodilator effect and should be repeated as required monitoring the sleeping respiratory rate (SRR). Addition of nitro-glycerine (vasodilator) reduces preload, decreases left atrial pressure and help in reducing pulmonary oedema. If the clinical signs are suggestive of cardiogenic shock or myocardial failure, Pimobendan I/V or Dobutamine CRI should be considered.

Introduction

One of the remarkable advancements in veterinary medicine recently is the application of interventional cardiology techniques to treat various congenital heart diseases. In dogs and cats, congenital heart diseases can be treated much more easily and with fewer complications using minimally invasive interventional approaches, rather than through traditional open-heart surgery. Among congenital heart diseases, treatment options are available for conditions such as PDA (Patent Ductus Arteriosus), AS (Aortic Stenosis), PS (Pulmonic Stenosis), VSD (Ventricular Septal Defect), ASD (Atrial Septal Defect), extrahepatic shunt, and stem cell applications. These diseases often cannot be significantly improved by medical treatment alone, making the use of the aforementioned interventional techniques essential for effective management.

Asiatic Elephant (Elephas maximus) is the national heritage animal of India. This particular species now exists in 13 Asian countries including India. As per 2017 census, India is home to an estimated figure of 29,964 elephants that is >60% wild population of the globe. Once distributed across the country, now they are squeezed to 14 states grouped in four regions ie., southern (14,612), north-eastern (10,139), east-central (3,128) and northern regions (2,085). There are 2675 captive elephants across different states under both private and government custody. This largest terrestrial mammal is protected under Seclude I species of Indian Wild Life Protection Act, 1972.

Both central and state Government are implementing short- and long-term programmes for health and well-being of captive as well as free-range elephants. The Central Government has established an ‘Elephant Cell’ at the Wildlife Institute of India (WII), Dehradun to provide technical inputs to the ministry for strengthening the efforts towards conservation of elephants. Add-on, the ‘Project Elephant’ was launched in 1991-92 in the Ministry of Environment, Forests and Climate Change to restore the elephant habitats and reduce their sufferings. Elephant habitats are notified as Elephant Reserves that are basic management units for elephant conservation and managements. A total of 33 Elephant Reserves has been notified in the country. A book titled, ‘Caring for elephants: Managing health and welfare in captivity’ is edited for field officers, veterinary professionals and elephant handlers.

The arid region in India is spread over in 38.7 million hectare land. Out of the total 31.7 m hectare lies in hot arid region and remaining 7 m hectare comes under cold arid region. The hot arid region occupies major part of northwestern India (28.7 m ha) and a small pocket (3.13 mha) in southern India. The north western arid region occurs between 22°30’ and 32°05’ N latitude and 68°05’ to 75°45’ E, covering western part of Rajasthan, north western Gujarat and south western parts of Haryana and Punjab. About 62% area of arid region falls in western Rajasthan and 20% in Gujarat. Haryana and Punjab together constitute 7% area of arid region. The state wise distribution of arid region is shown in Table 3.1. Further our discussion is based on the majority area of arid region, belonging to Rajasthan, Gujarat, Punjab and Haryana.

Soil sampling is an integral and essential component of soil fertility evaluation and nutrient management research. The effectiveness of soil sampling is a prerequisite for soil testing to achieve its goals for efficient nutrient management and plant nutrition for improved yield and quality. The underlying basis for soil sampling is that a soil sample taken represents the “population” which may be a plot, field or a watershed. It further implies that nutrient status of the representative soil sample(s) determined in a laboratory would reflect nutrient status of a plot, field or watershed and is of interest for correcting nutrient disorders in the field or watershed.

The most important factor that influences the effectiveness of soil sampling is soil heterogeneity. However, in a relatively homogenous group of fields or plots, a small number of samples may be sufficient to represent the population to a more heterogenous group of fields that would require more number of samples to represent the soil population. Whenever, soil samples to be taken in field following points needs to be kept in mind.

2.1 Introduction

Automation of agricultural field with IoT and microcontroller is new era of technology. Arduino based solution to agricultural field is open source platform. It can be used for developing data loggers and new sensors environment. The rate of solutions with Arduino and sensors is high as it is open source platform. The use of Arduino in agriculture can accelerate the growth of automation. Arduino can be interfaced with sensors like:

• Temperature
• Humidity
• Soil Moisture
• Water Pressure
• Water Flow and Water Metering
• Rain Gauge

5.1 Introduction

The chapter describes the development of local web server on the laptop, to control the actuator from the server. Web server is used as remote control for the system. The system is designed to control water pump with respect to the soil sensor values. It comprises of NodeMCU, soil sensor, LCD, two relay boards for pump in and out the water from field.

Land resources units or major land resources units are the suitable segmentations of land, based on available natural resources, their potential use and management. Irrespective of terms used by different organizations or institutes, these units have spatial dimension and represent a composite unit for interstate, regional, and national level planning. A typical meaning of these land units can be related to terms like land system. Christian and Stewart (1953) of the Commonwealth Scientific and Industrial Research Organization (CSIRO), Australia devised the ‘Land system’ as the composite mapping-unit which has been defined as “an area or group of areas, throughout which there is a recurring pattern of topography, soils and vegetation”. This system is based on the premise that the land system is expressed on aerial photographs by a distinctive pattern and, as such, the land system can be directly mapped from aerial photographs (Christian and Stewart 1953, Mabbutt and McAlpine 1965). It is a fact that during those period, aerial photographs were more frequently used and studies related to land systems used technique of aerial photogrammetry. As per USDA (United States Developmental Agency), the typical segmentations are; (1) Land Resource Region (LRR), which is the highest level in the hierarchy (2) Major Land Resource Area (MLRA), the second highest level and generally representing broad landforms or a geologic region at a small scale and (3) the Land Resource Unit (LRU).

Introduction

Soil organic carbon (SOC) content plays a key role in maintaining soil fertility in agricultural farm. At present, there has been great interest to reduce the atmospheric CO₂ level through a chain of increasing vegetation carbon pools first and then to store them as soil carbon pool. To quickly assess the soil carbon restoration programmes, it is important to know how much soil carbon is stored in an agricultural farm through adoption of different land management practices. Average SOC content is considered in most soil carbon pool calculations in spite of its large spatial variation in landscape, and thus leads to inaccurate estimate of SOC stock for an area. Therefore, reliable estimates of SOC pools and their spatial variability are essential to establish the soil carbon sequestration programmes at different landscape scales. Here, a case example of assessing the spatial variation at a typical agricultural farm is discussed. The experimental field was located at Badoda village of Jaisalmer district, Rajasthan covering an area of about 76.12 ha. The farm lies between 265^o2′30″-26°53′30″ N and 71°12′15″-71°13′15″ E (Fig. 24.1) and 20 km away from Jaisalmer city in eastward direction. Elevation of the farm ranged from 232 m to 248 m above mean sea level with an overall slope from south to north. Among total area of the farm, 85.38% area was under cultivation in four main blocks: block A, B, C, and D whereas rest area was under grassland.

The recent concept of intellectual property emerged in England in the 17^th & 18^th centuries. The term Intellectual Property came in use in the 19^th century and in the late 20^th century. As of now the Intellectual Property has occupied common position in the global legal systems. There are different forms of Intellectual property rights which include patents, copyright, industrial design, trademarks, plant variety rights, trade dress, geographical indications and in some jurisdictions trade secrets too

Major International Treaties Related to IPR & TRIPS

There are different forms of intellectual property like Patents, Copyright, Trademarks, Industrial design, Plant Varieties etc. Various meeting and conventions were also convened for the protection in these intellectual properties at international level from time to time and to bring uniformity and coordination among member countries. These were reflected in different treaties, which are mentioned below. Among all these treaties/conventions the agreement on Trade related aspects of intellectual property rights (TRIPS), which runs under aegis of World Trade Organization is most influential, comprehensive and inclusive of all.

The International Union for the Protection of New Varieties of Plants or UPOV is an intergovernmental organization with headquarters in Geneva, Switzerland. UPOV was established by the International Convention. The objective of the Convention is the protection of new varieties of plants by an intellectual property right. The Convention was adopted in Paris in 1961 and revised in 1972, 1978 and 1991. UPOV aims to encourage the development of new varieties of plants for the benefit of society by codifying intellectual property for plant breeders.

The International Union for the Protection of New Varieties of Plants (UPOV) based in Geneva, Switzerland and established in 1961 is an intergovernmental organization to provide and promote an effective system of plant variety protection, with the aim of encouraging the development of new varieties of plants, for the benefit of society. For plant breeders' rights to be granted, the new variety must meet four criteria under the rules established by UPOV as discussed earlier also.

Components of biodiversity are all the various forms of life on Earth including ecosystems, animals, plants, fungi, microorganisms, and genetic diversity. Ecosystems, species and genetic resources should be used for the benefit of humans, but in a way that does not lead to the decline of biodiversity. Substantial investments are required to conserve biodiversity, but it will bring significant environmental, economic and social benefits in return. To address these challenges various initiatives were taken in the past such as Ramsar Convention on Wetlands in 1971, Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES) in 1973, 1979 Bonn Convention on Migratory Species (CMS) in 1979.

The ITPGRFA is a comprehensive international agreement which is poised at guaranteeing food security through the conservation, exchange and sustainable use of the world's plant genetic resources for food and agriculture, instead of fair and equitable benefit sharing arising from its use. It also advocates Farmers' Rights.

13.1. Genesis of the Treaty

The conservation and sustainable use of plant genetic resources for food and agriculture are keys to feed global growing population in the coming future. The Commission on Genetic Resources for Food and Agriculture was established in 1983, and the voluntary International Undertaking on Plant Genetic Resources was adopted here in. Another major step was taken in 1996 with the adoption of the Global Plan of Action at the Leipzig International Technical Conference on Plant Genetic Resources.