eChapters

1 Analysis of Cream

 Sample Preparation

Warm the cream sample to 40 - 50oC if the sample is very viscous. Then pour from one vessel to another 3 to 4 times to mix well. Sample should be tested daily and always within three days after collection since enzymatic deterioration of the cream will cause low tests.

1.1 Determination of Fat Content in Cream

 1.1.1 Gerber Method

 Introduction

Sulphuric acid is added to cream in order to dissolve the protein and other solids and create heat which liquefy and de-emulsify the fat. Iso-amyl alcohol is added to aid the separation of fat. Upon centrifugation, fat being lighter will be separated on top of solution in the cream butyrometer.

Enzymes (biological catalyst) can be defined as a substance which initiates or fastens the reaction rate, without being consumed during the reaction. Enzymes function as a catalyst in various biological processes taking place in all life forms. Although enzyme synthesis takes place inside living cells, they can even function as a catalyst in the in vitro processes, which makes it suitable for many industrial applications. For product formation in any chemical reaction, reactants or substrates has to overcome the transition state.

Fourier transform infrared (FTIR) spectroscopy is one of the most effective methods for determining the functional groups and potential molecular interactions between membrane chemical molecules. Understanding of (infrared) IR absorption band placements in the spectrum as wave numbers can be used to identify the various chemical constituents (such as aromatic amides) that may not be visible by X-ray photoelectron spectroscopy.

Quality assurance is fundamental to producing safe, consistent, legally compliant, and commercially successful ice cream. This chapter presents a comprehensive QA framework covering supplier approval, incoming-material inspection, process monitoring, laboratory testing, microbiological control, sanitation, finished-product release, traceability, market quality, and corrective and preventive action. It explains the relationship between quality assurance, quality control, food safety, documentation, and management systems such as HACCP and ISO-based frameworks. The chapter also examines QA department structures, monthly reviews, audits, digitalization, quality culture, and performance indicators. By integrating prevention with measurement and accountability, the chapter demonstrates how effective QA can protect consumers, reduce losses, improve consistency, and strengthen profitability.

This chapter introduces approaches for converting egg-processing residues and by-products into useful materials and products. It examines eggshell composition and applications, eggshell membrane and its biomedical and industrial potential, utilization of surplus and inedible egg material, and extraction of high-value components such as lysozyme and IgY. Waste management, sustainability and circular-economy approaches are also discussed. The chapter emphasizes that successful by-product utilization depends not only on technical feasibility but also on economics, scale, infrastructure, biosecurity and appropriate markets.

Electricity is one of the fundamental phenomena of nature. Electricity or electric current is caused due to the movement of charged particles i.e., electrons. Due to the potential difference between two points in a conductor, electron movement takes place. The potential difference between two points is caused due to the difference in charge density. Electron tends to flow from lower potential to higher potential which causes electricity to flow. In general, the electrons flow from cathode (negative) to anode (positive) but in a wire electricity, electrons flow from anode to cathode when connected to a battery or any electricity source.

Abstract

This chapter presents the principle of fluidisation and its use in drying of food materials. Being a convective drying process, capacity and kinetics are limited by process conditions and material properties. The influences are discussed as well as presenting the main equations that describe drying in fluidised beds. The main focus lies on hot gas drying, additionally superheated steam drying as well as atmospheric fluidised bed freeze drying and microwave-assisted fluidised bed drying are presented and discussed.

Abstract

Mushrooms are the fruiting bodies of fungi, a group of organism that is different from fruits and vegetables. They are a rich source of good quality protein having more essential amino acids and vitamins with low calories. There are about six commonly known edible mushrooms throughout the world with button mushroom (Agaricus bisporous) being the most familiar and commercially important variety. Mushroom is a highly perishable product with a shelf life limiting to a few days. Soon after harvest, it starts to undergo physiological disorders like shriveling, wilting, liquefaction, browning, textural as well as weight losses and flavour changes. The main responsible factors for the quality deterioration after harvest are respiration rate, browning, bacterial spoilage of mushrooms, temperature and relative humidity. Because of its limited shelf life under ambient conditions of temperature and humidity, the preservation of mushrooms is a matter of concern. Various techniques have been involved in the extension of shelf life of mushrooms including chemical treatments, cooling, modified and controlled atmospheric storage, radiation, drying etc. Drying is one of the most accepted and effective methods for the long term storage of mushrooms. Mushrooms preserved by drying have a pleasant flavour and are used as an important ingredient in several food formulations including salads, instant soups, meats and rice dishes. Various drying processes like sun drying, hot air drying, freeze drying, vacuum drying etc. can be used for the long term storage of mushrooms. As mushrooms are sensitive to temperature, determining the right drying method is the key for the successful and effective operation of the system. The different operating conditions involved in various methods of drying affect product quality considerably. The effect of drying methods on the process and the quality of the dried mushroom is explained in detail in this chapter.

Abstract

Vitamin C is one of the most important phytochemicals and can be found in almost all fruits and vegetables. Since it covers almost all eventualities, being water solute and prone to leaching from cells, thermally labile (one of the most labile vitamins), pH-, metal ion-, light-sensitive, as well as readily oxidizable by ascorbic acid oxidase, vitamin C is selected as the most frequently measured nutrient to evaluate the nutrients loss during thermal processing. It is generally accepted that if vitamin C is well preserved, the other nutrients are also well retained. Higher retention of vitamin C in processed products is highly desired. Herein this chapter reviews the degradation mechanism and kinetics of vitamin C of fruits and vegetables during thermal processing, such as blanching, drying, cooking and frying. It starts with an overview of the functions of vitamin C in human’s health. Then the current status for understanding of the degradation kinetics of vitamin C during thermal processing was outlined, followed by the discussion of its degradation mechanism. At last, potential strategies or alternatives to keep vitamin C are identified and discussed.

India has a mega animal bio-diversity, which is manifested in the form of availability of a large number of species, and a variety of breeds even within species. India is bestowed with rich and varied domestic animal biodiversity having a total of 32 breeds of cattle and 10 buffalo breeds. The diversity of livestock is not only accidental, nor purely natural; it is more the outcome of thousands of years of continuous selection. The trends in livestock population over the last three decades have shown that, there is significant increase in the population of buffaloes i.e. from 60.5 crore in 1973 to 96.9 crore in 2003, while cattle population has increased from 180.1 crore to 226.1 crore in the same period. The share of buffaloes, in the bovine population increased from 22 to 32 per cent while that of cattle has shown declining trend. Within the cattle, there has been marked shift from work animals towards milch animals which has resulted in loss of important draught breeds of cattle. Further indiscriminate crossbreeding has resulted in significant decline in number of important milch breeds like Sahiwal.

The choice of hormones and drugs acting on reproductive system are important as therapeutic agents during reproductive disorders like anoestrus, metritis, pyometra, repeat breeder, retention of fetal membranes, delayed puberty, ovarian disorders etc.  The administration of specific chemotherapeutic agent is of paramount importance in treating reproductive disorders in dairy animals.  The dosage, route, frequency and indications of chemotherapeutic agents acting on reproductive system are mentioned in tabular form as below.

Non-specific uterine infections reduce the reproductive efficiency of livestock.  They are called nonspecific because the initial colonizing bacterium is not known and the specific bacteria causing the signs of infection are not known.  Even though numerous bacteria, in a variety of combinations, have been isolated from infected uteri, Corynebacterium pyogenes and Escherichia coli are usually associated with uterine infections in cattle and sheep.  Empirical therapeutic approach, prescriptions without scientific validation (extra-label use) or inadequate clinical judgment itself to ascertain the nature of uterine infection, obviously due to lack of diagnostic tools are the prime reasons for therapeutic failure for the unsuccessful treatment of uterine infection by large animal practitioners.

Estrous cycle

After puberty the female enters a period of reproductive cyclicity, which continues throughout most of her productive life.  Estrous cycle is the rhythmic sexual behavioral pattern exhibited by the female, which begins at one estrus (heat) and ends at subsequent estrus.   It is the period between two estruses of the regular cycle.  The reproductive activity of the female is maintained by the co- ordinated functioning of different organs of reproduction (Table: 1.1). Estrous cycle provides repeated opportunities for the females to become pregnant. When conception does occur, the female enters a period of anestrous during pregnancy, which ends after parturition (giving birth) and uterine involution (repair and returning to normal size).

Faulty estrus detection is the most important problem in reproductive management.  There are two requirements for good estrus detection: a skilled observer must spend sufficient time to make observations and cows must show overt signs of estrus during the period of observation.  There are various ways to detect the estrus signs in cows and buffaloes as detailed below.

1.    Palpation of reproductive organs:  Routine rectal examination of all cows between 30-40 days after calving and individual cow that has not been inseminated within two months after calving should be encouraged.   This is to confirm that the reproductive tract is normal and to predict when the next estrus will occur or to identify cows for prostaglandin treatment when estrous cycles are occurring, but estrus has not been detected. 

2.     Teaser bulls:  Teaser bulls are useful in identifying estrus in large number of animals especially buffalo cows.   Teaser bulls can be prepared by vasectomy or by applying apron to a bull. 

3.     Heat detector animals:  Sexually active animals like bulls, cystic cows, hormone treated steer, cows and heifers can be used to identify estrus cows.    They may be fitted with halters containing ink- filled reservoirs and ball paint pen type devices (chin ball markers) that will mark animals that mounted or they can be used without these devices to increase the sexually activity and make visual detection programs more effective.

Synchronization of estrus is making a group of cows to come into estrus at the same time.  Effective estrus detection requires much time, labour, skill and expense and it is a major limiting factor in artificial insemination programme in cattle.  It was the principal stimulus that led to research in the development of progestational and prostaglandin compounds. 

Advantages

1.     Improve the efficiency of heat detection

2.     Management tool, better planning of breeding activities and wider use of artificial insemination 

3.     Solve problem of unobserved estrus

4.     Maintain short calving interval

5.     Overall improvement in herd productivity and reproductive efficiency

6.     Controlled lactation

7.     Management of replacement heifers

8.     Useful in embryo transfer programmes

9.     Reduce labour

10.     Possibly reduce involuntary culling for reproductive reasons

There is no rapid direct test to determine fertility of semen sample. During freezing and thawing of semen 20-80% of the spermatozoa become immotile or die. In addition to this, there may be damage to spermatozoa by disintegration of sperm cell membrane and acrosome leading to loss of intracellular enzymes.   The only reliable method to evaluate the semen is to inseminate a cow and get a calf at the end of gestation period.  Now-a-days new methods have been developed for in-vitro assessment of fertility of spermatozoa.  Some of these methods are exhaustive, time consuming, need for a full fledged analysis and difficult under field conditions.

Reproductive process involves many events that have to occur in a well balanced manner if satisfactory fertility rate is to be achieved. Any adverse  effect of  physiological, morphological  or environmental effect on the male  or  female may result  in failure  of  fertility  or reduced fertility  depending  on the severity of the cause. Apart from this, the role of person who stands between the cow and the bull play an important role. The efficiency of the person handling the semen and depositing in the reproductive tract of domestic animal will influence to a great extent on the conception rate in A.I. programme. It is observed that certain bovine population fails to conceive at a single service or series of services.   Some times there is loss of conceptus during gestation or produces a dead calf or weak calf that will die soon after birth.
 
Consequences of these factors affect cows and may have long calving interval, abortion or sterility, all of which tend to decrease the average daily production of milk.

Livestock diet or ration may be made up of one or several ingredients depending on the species of animal, their physiological state, source of feed and its nutritional composition, nutritional requirements of the animals and voluntary intake of the feed. Chemical and nutrient composition with each ingredient varies tremendously and no single feed ingredient is complete in its nutrient composition with respect to animal’s requirement.  Therefore, animal feed has to be balanced properly to supply required nutrients in appropriate quantity.  Feed formulation is an art of quantitative application of science of nutrition for the optimum supply of all the essential nutrients through actual feeding of animals. This exercise may be as simple as mixing of few ingredients with simple mathematical calculation or preparation of least cost balanced ration by computer programme.  Since the expenditure on feeding is substantial in livestock production, feed formulation needs special attention of nutritionists or feed technologists. The instruments and equipments used for feed formulation and estimation of micro minerals and ingredients like Feed grinder and mixer of capacity 75 kg, Fibertech for fiber estimation, Hammer mill-particle size reduction, Mixer and grinder for feed and fodder (complete feed preparation) of capacity 250 kg, Roughage processing-Chaff cutter, Soxhtech for fat estimation, UV-vis-Spectrophotometer and Voltameter for micro mineral estimation are shown in Fig. 3.1 to 3.8.

Buffaloes, though have great production potential, do suffer with many reproductive problems viz.  delayed age of puberty and  sexual maturity, weak / silent estrus, longer inter-calving period,  seasonality in breeding, poor libido  and poor semen quality. Little information is available about the buffaloes as compared to cattle because all the time we try to apply the knowledge available about the cattle to the buffalo.  Some comparative reproductive parameters in cow & she buffalo & cow bull and buffalo bull are depicted in Table 8. 1 and Table 8.2 respectively.

The various steps involved in frozen semen technology are vital as the quality of semen determines the conception rate.  The key factor enabling the successful long term storage of frozen spermatozoa is maintenance at ultra low temperature.  Effective semen storage depends heavily on good identification and stock control.  Frozen semen can be easily damaged in the process of repeated handling to establish identity.