eChapters

Microorganisms (or microbes) are organisms too small to be seen with the naked eye. The science of microbiology is the study of microorganisms, their properties, classification, growth requirements, method of reproduction and distribution in nature. These organisms range in size from tiny viruses to larger bacteria, protozoa, algae, fungi and some of the microscopic worms (helminthes). They are present everywhere in our environment, in our homes, on our bodies, on the things we touch and on the food we eat. They are important for human survival on Earth, playing an essential role in many processes that maintain life. In nature, microorganisms carry out a number of important functions.

1.They are responsible for the decomposition of organic matter.

2.Recycling of nutrients that help to maintain the balance of chemicals in the soil.

3.Special nitrogen-fixing bacteria live symbiotically with certain plants, absorbing nitrogen from the air and converting it into compounds which can be used by the plant for growth.

4.Some algae are capable of photosynthesis, using energy from the sun to convert carbon dioxide in air into carbohydrate.

5.Marine microorganisms form the basis of the food chain in lakes, rivers and oceans.

6.Certain microorganisms are used in the manufacture of food.

7.Pharmaceuticals and drugs.

8.Industrial processes.

9.A valuable tool for scientific research.

10.Use in laboratory experiments to understanding metabolic pathways and in the discovery of the genetic code.

11.1 Introduction
“Homogenization” is the method of mechanical treatment producing a stable emulsion of milk fat and milk serum. Homogenizer is the name given to the equipment employed for this aim. The fat globules in milk range in size from 1 to 20 microns; the average is 4 to 6 microns. The homogenizer reduces the size of the fat globules in milk or cream passing through it. The most of them are less than two microns. After homogenization, no cream line develops on milk; churning homogenized cream cannot generate butter grains. Moreover, milk like cream’s viscosity is changing, which increases the resistance the smaller fat globules face. The increase in viscosity is ascribed to the fact that more milk protein will be deposited on the surfaces of more fat lobules. Homogenizing milk changes its clotting characteristics, so the curd made with rennet or pepsin is supposed to be softer. Because lipolytic enzymes adsorbed on the extra surfaces provided by the more numerous tiny fat globules hydroly fat, homogenized milk or cream may become rancid more quickly than untreated goods. The milk or cream must be pasteurized either right before or right after homogenization to prevent these changes. Usually, the milk is homogenized after main heating and then pasteurized. Driven via a small valve against hard surfaces, heated milk is under pressure of 2500 psi and in a second stage under 500 psi. With a 1/10000 inch diameter, the aperture the liquid is driven through is quite small. Mechanical force milk through a narrow passage at high speed produces homogenized milk. This produces a stabile fat emulsion and breaks down the fat globules in milk into considerably smaller ones. Homogenization reduces the inclination of the fat globules to cluster and merge into cream. Milk is an emulsion whereby fat globules are distributed across a constant skim milk phase. Raw milk left to sit will finally cause the fat to climb to the top and form a cream layer. By means of a small opening under high pressure, homogenization mechanically breaks down the fat globules. While increasing their number and surface area, this lowers the average size of the fat globules. Practically speaking, the net effect is a substantially decreased inclination for creaming of fat globules. Three elements contribute to this increased stability of homogenized milk: a decrease in the mean diameter of the fat globules (a factor in Stokes Law), a decrease in the size distribution of the fat globules (causing the speed of rise to be similar for most globules such that they do not tend to cluster during creaming), and an increase in density of the globules (bring them closer to the continuous phase) owing to the adsorption of a protein membrane. < Heat pasteurization also breaks down the cryo-globulin complex, which often clusters fat globules causing them to increase. The homogenizer consists of a high-pressure pump with an adjustable opening based on a minute orifice. High pressure force of fluids through the orifice causes a notable change in the physical characteristics of the treated product and generates a very intimate combination of the fluid components. Annexure- II has a Homogenizer schematic.

1. Introduction

Raw milk is an emulsion. It is a mixture of milkfat globules, Solid Not Fat (SNF) and water. Over time, milkfat globule separate out from milk serum and comes to the surface of milk as a layer of cream due to the low density of milkfat or due to heating of milk. When milk is heated rapidly and continuously, some of the water in it evaporates from the surface. This exposes proteins and fat molecules, which bind and dry out and forms a skin like film on the surface. 

13.1 Introduction

Hospitality industry is the second largest industry in the world. It is well diversified and can be chosen as per the interest. Hospitality management involves planning, organizing, directing and controlling human and other resources within the different segments of hospitality industry i.e. lodging, restaurant, travel and tourism, institutional management, recreational management, meeting and convention planning industries. Although, these all are separate but related segments of the hospitality industry and are interrelated to provide good and efficient services to the guests and users. Hospitality industry provides services which are need oriented and differ in various parts of the world as per regions and the traditional styles.

The first building created specifically for hotel was ‘City Hotel’ with 73 rooms at 115 Broadway, New York in 1794. Pallonjee Pestonjee established the very first western-style hotel in India, “The Bristol Hotel” in Mumbai in 1840. According to the report of the department of Tourism, Government of India, at the end of the year 2004, there were 167 Five star hotels with approx. 28,900 rooms, whereas 126 Four star hotels, with 8,831 rooms [2]. To expedite the ongoing process of classification/re-classification of functioning hotels in one to three star categories, five Regional Committees have also been set up in Delhi, Mumbai, Kolkata, Guwahati and Chennai to conduct inspection [3].

According to Futter (2001) it is very important the way we cook our food. If the cooking is inadequate it causes food poisoning. While cooking there can be cross contamination starting from raw foods. It includes hands or utensils which can cause food poisoning. Most foods, especially meat, poultry, fish and eggs should be cooked thoroughly to kill most types of food poisoning bacteria. Generally the food should be cooked to a temperature of 75 °C or hotter. When food is cooked, it should be eaten promptly, kept hotter than 60 °C or cooled, covered and stored in the fridge or freezer. Some people are more at risk from food poisoning than others. In order to buy safe food in the market like buying pre-cut produce as a half a watermelon or cut veggies from a salad bar, it is important to choose items that are refrigerated or surrounded by ice. They must be kept in separate bags. FDA (2019) has studied the contamination in lettuce. Shopping at a farmer’s market is a great way to get locally-grown, fresh fruit, vegetables, and other foods. However, safety of these produce needs to be checked. Many markets have their own food safety rules and vendors must comply with them along with the government regulations. Fruits and vegetables must be washed thoroughly under running water just before eating, cutting or cooking. Fruits and vegetables must never be washed with soap or detergent or using commercial produce washes. Even if it has to be peeled before eating, it is still important to wash it first. Any bacteria present on the outside of items like melons can be transferred to the inside when it is cut or peeled. They should be refrigerated within two hours after preparation. The juice or cider must be treated (pasteurized) to kill harmful bacteria. Pregnant women, children, older adults and people with weak immune systems should drink only pasteurized or treated juice. Raw milk can harbor dangerous microorganisms like Salmonella, E. coli, and Listeria. World India (2017) has looked into the food regulations. These can pose serious health risks. Pregnant women, older adults and people with weakened immune systems are at higher risk for illness caused by Listeria. One source for these bacteria is soft cheese made from unpasteurized milk. It is vital to check the label to make sure that it is made from pasteurized or treated milk. Make sure that eggs are properly chilled at the market. FDA requires that untreated shell eggs must be stored and displayed at 45°F. While buying eggs it needs to be ensured that the eggs are clean and the shells are not cracked. Meat should be properly chilled even in the market and kept in closed coolers with adequate amounts of ice to maintain cool temperatures. If it is brought in an insulated bag or cooler during transit it is very beneficial to keep the quality. It should be always kept separately from your other purchases, so that the juices from raw meat (which may contain harmful bacteria) do not come in contact with produce and other foods. Essays (2018) have written a very comprehensive overview of food safety.

Today, organic and local foods are preferred as they use less pesticides or fertilizers. However, these foods, like others, can be exposed to harmful bacteria during the growing and harvesting process. The farmers and distributors must use good sanitary practices to minimize food contamination. Consumers should cook food properly. The street food vendors are a traditional and indigenous fast food approaching in most countries of the South-East Asia Region. As these are economical they cater to millions of consumers. Among safe street foods there are many contributing factors that are associated with the safety of street food. WHO had conducted a survey in 1993 covering 100 street foods in 100 countries around the world. It revealed that the major health threat comes from raw and undercooked food, infected food handlers and inadequate hygiene practices in processing and storing such food. If the street food is cooked well and is served hot, then the chances of food poisoning is less. The water quality, hygienic conditions and the level of cleanliness especially in the summer season are the major factors for food poisoning. However improvements in physical infrastructure and hygienic conditions are growing as consumers are also demanding quality food. Michael et al., (2017) have looked into organic versus conventional foods.

INTRODUCTION

The concept of hurdle technology has been developed as a new way to realize safe, stable, nutritious and economical foods. It employs combination of different preservation techniques to achieve a multi-target, mild but reliable preservation of foods. The application of hurdle technology in food preservation is increasing rapidly worldwide. Over the years, insight into the hurdle effect has been broadened and the application of hurdle technology has been extended. A better understanding of the occurrence and interaction of food preservative factors (hurdles) in foods is the basis for improvements in food preservation. If the hurdles for the microbes are known, the microbiological stability and safety of this food might be optimized by changing the intensity or quality of these hurdles. Using the proper combination of hurdles, it is possible to improve not only the microbial stability and safety but also the sensory and nutritive quality as well as the economical aspects of food. The hurdle concept is also referred to as food preservation by combined methods, combined process, combination preservation or combination processing techniques. The hurdle effect is of fundamental importance for the preservation of foods. The illustration of hurdle effect was first introduced by Leistner in 1978. Based on the understanding of hurdle effects, the concept of hurdle technology was derived (Leistner 1985).

Safe, wholesome and nutritious food is vital for sustenance, good health, food and nutritional security and augmentation of sustainable development of an individual. Also, safe food helps in the growth of the national economy and enhances the trade and tourism sector. Foods that are unsafe containing various kinds of microorganisms can cause chronic diseases or illnesses varying from diarrhea to cancer.

Dairy equipment and utensils with which milk and milk products come into contact are the main sources of contamination in the products. By the use of effective equipment cleaning and sterilization methods on routine basis, milk of high bacteriological quality can be consistently produced. In composite milk plant, a variety of equipment and utensils are used. The use of stainless steel for the fabrication of dairy utensils and equipment provide highly polished and hard smooth surface that can be cleaned readily, apart from showing resistance to corrosive action of detergents and bactericidal solutions. Utmost care is taken to avoid dents and scratches, which could be the possible focal points of subsequent contamination. To overcome all these problems aluminium and stainless steel vessels and equipment present an ideal situation.

Abstracts

To ensure safe food and adequate sanitation programs, the equipment used for processing of food products must be designed, fabricated, constructed, and installed according to sound hygienic design principles. This ensures the equipment can be adequately cleaned and sanitized, and that surfaces are resistant to daily exposure to corrosive food products and cleaning/sanitizing chemicals. This chapter focuses primarily on fundamental principles of hygienic design, which protect the product from microbial, chemical, and foreign-body contamination. This is followed by a discussion of general aspects of hygienic construction and design of food equipments, the selection of materials of construction and specification of surface finishes, fabrication, installation and cleaning and sanitization. Quality of raw materials or foods ingredients, proper processing, packaging and storage of finished products decides the quality of the food products. Planning for plant and equipment layout, its design, services (water, air, refrigeration, steam), waste disposal etc. are some of the critical factors, which contributes to quality. In addition, the food processing equipment must comply with strict standards and regulations, which are necessary for securing the quality and safety of the food products to the consumers.

The Concept of ‘Clean Milk’ Production

Although milk is nature’s most ideal and perfect single food both for the newborn and the mature human beings, the possibility of it being a source of biological and chemical hazards cannot be excluded. In view of the immense dietary importance of milk, the need for production of safe, clean and wholesome milk is emphasized. The term ‘clean milk’ does not mean milk in which all visible dirt is absent or milk from which it has been removed; rather it does denote raw milk from healthy animals, that has been produced and handled under hygienic conditions; that contains only small number of harmless bacteria and that possesses a good keeping quality without being treated by heat. In other words, the unhygienic production of milk may lead to inferior keeping quality as a result of rapid microbial multiplication under favourable storage conditions apart from serving as a potential health hazard. These microorganisms cause the milk to sour quickly, putrefy and develop undesirable flavours, colour defects, ropiness and bitterness. On the other hand, the pathogenic microorganisms, which may gain entry through various sources, may cause many diseases to the consumers.

Ice cream over the years has come to acquire an easy acceptance as a healthy, nutritious and hygienic food for the aged, sick, growing children and adults. Its popularity, as demonstrated on all types of occasions is growing fast and its potential appears to be unlimited.

Gone are the days when the word “Ice Cream” was synonymous with strawberry and vanilla flavours or the innocuous orange. Ice cream is available in many forms, flavours and packages. Today, there are exotic variations to titilate the palate, such as Jingle Bells, Goody goody Gum Drops, Champagne Snowballs, Mr. Frost, Tutty Fruity, Butter Scotch, etc, waiting to launch customers on a gastronomical voyage.

It is uncertain how long ice cream has been produced, however, the International Association of Ice cream Manufacturers (1966) traces the beginnings of ice cream and frozen desserts to iced drinks enjoyed by Alexander the Great in the fourth century B.C. down through the years Nero in the first century A. D., Marco Polo in the 13^th century through Italy and France to England in 1643-1649, and to America around 1700. From a very old writings it has been learnt that the Chinese liked a frozen product made by mixing fruit juices with snow, what we now call water ice. It is believed that ice cream evolved from iced beverages and water ices that were popular in the medieval period. This technique later spread to ancient Greece and Rome, where the wealthy, were partial to frozen desserts.

After disappearing for several centuries, ice creams in various forms reappeared in Italy in the Middle Ages, most probably as a result of Marco Polo returning to Italy in 1295 after a 16-17 year stay in China, where he had learnt to appreciate a frozen dessert based on milk. There is no gainsaying the fact that the true origin of ice cream cannot be proclaimed with a fair measure of certainty but, all the same, the Italians claim that Benaroo Bountalenti, a resident of Tuscany, invented ice cream in the early 1300s. From Italy ice cream spread over Europe during the 17^th century, and for long remained a luxury product for the royal courts. Italian ice cream was very popular in London around 1870.

A greenfield ice cream project involves much more than installing production machinery. It requires integrated planning of the business model, market opportunity, capacity, product portfolio, plant layout, process technology, refrigeration, utilities, packaging, cold storage, quality systems, regulatory compliance, distribution, working capital, and future scalability. This chapter presents a practical framework for evaluating new ice cream manufacturing projects, with particular attention to a representative 1000 LPH facility. Different project scales and business models are considered along with major plant and machinery requirements. The chapter also highlights common project-planning mistakes and best practices. It helps entrepreneurs and investors assess technical feasibility, operational readiness, financial requirements, scalability, and long-term commercial viability.

India is emerging as one of the most dynamic ice cream markets, with growing demand driven by urbanisation, rising disposable income, premiumisation, regional flavours, modern retail, quick commerce, and expanding cold-chain infrastructure. This chapter examines the evolution and structure of the Indian ice cream market, major growth drivers, consumer segments, competitive conditions, and emerging opportunities. It also considers important challenges including seasonality, freezer investment, cold-chain reliability, SKU complexity, regulatory compliance, working capital, and market returns. The chapter provides strategic guidance for entrepreneurs and manufacturers on market entry, positioning, product portfolios, distribution, and sustainable growth in India’s increasingly competitive ice cream industry.

18.1 Introduction
Ice cream is produced by freezing a pasteurised mixture that is derived from milk or milk products, with or without nutritive sweeteners and other approved non-dairy constituents, in accordance with FSSAI standards. Except for softserve ice cream, which may remain malleable, this product must freeze solid and may contain air. Ice cream is manufactured using bovine or buffalo milk, cream, or milk products. It may also contain cane sugar, eggs, fruits, fruit juices, preserved fruits, nuts, chocolate, edible flavours, and approved colours. Up to 0.5% by weight of stabilisers and emulsifiers are permissible. The mélange must be heated to the appropriate temperature prior to freezing. A minimum of 10% milk fat, 3.5% protein, and 36% total solids must be present in the product. However, the proportion of milk fat may be reduced to no less than 8% by weight when any of the aforementioned preparations contains fruits, almonds, or both. A declaration to that effect must be included on the label, and starch may be added to a maximum extent of 5%.
18.2 Classification
Some of the frozen desserts can be classified as follows.
1. Plain: Plain ice cream has minimal coloring and flavoring, such as vanilla and coffee.
2. Chocolate: Chocolate ice cream is flavored with cocoa or chocolate
3. Fruit: Fruit ice cream contains fruits like strawberry, apricot, pineapple, mango, or banana, which may be fresh, frozen, canned, or preserved.
4. Nut: Nut ice cream includes nuts like almonds, pistachios, walnuts, or cashews, with optional additional flavoring or coloring.

SOURCES OF LOSSES
In most of the cases of excessive fat or product loss, a combination of several factors rather than any single factor are responsible.
 
Inaccuracy in weighing: Receiving room scales should be checked regularly with a set of weights. The check should be made immediately before weighing operations begin. Improper drainage of the weighbowl will permit a carry over into the next batch dumped, resulting in incorrect weighing. Errors in weight in bulk handling methods may be caused also by improper leveling of the tank, incorrect calibration of the measuring rod, or carelessness on the part of the person taking the measurement.
 
Inaccuracy in sampling: Failure to collect representative samples may sometime cause sizeable losses. Milk should be properly agitated before taking samples. The composite sample should be preserved and refrigerated and should be protected from mould growth.

Livestock is considered one of the essential variables in the revenue of a farmer. The sector (excluding fisheries) contributes 30 per cent to India’s agriculture gross domestic product, and it is valued at more than Rs. 9 lakh crores. India is also one of the largest producers of milk in the world; around 70 million rural households are engaged in professions linked to dairy. India is also the second largest exporter of bovine meat after Brazil. This sector provides a substantial income to farmers and supports the economy by generating enough demand in the rural and semi-urban regions. This animal husbandry sector comprising dairy and poultry is extremely valuable for farmer’s income security, and Covid-19 has the potential to disrupt this sector.

In developing countries such as India, where dairy farming is one of the most important sources of livelihood for 60 million rural households, 80 per cent of whom are landless, small and marginal farmers who depend on dairy for daily income, the crisis is extreme. Thus, even a small decline in the growth of the dairy sector significantly affects a large chunk of our population which are already vulnerable due to low income, poor savings, and lack of livelihood alternatives. Small production in multiple households leads to more reliance on efficient transportation for procurement. In addition to this, the Indian dairy sector is still dominated by the unorganized sector which handles more than 50 per cent of the marketable surplus. Milkman and vendors collect a small quantity of loose milk from different households and then supply it to urban consumers, roadside eateries, hotels, and sweetshops. Closure of shops and restrictions on transportation has ruthlessly disturbed this arrangement. Even in the formal sector out of the total milk processed 65 to 70 per cent is sold as liquid milk which is immediately vended after packing and only 30 per cent is processed into other dairy products like ghee, butter, cheese, curd, ice-cream, milk powder, etc. Thus, the formal sector also lacks sufficient infrastructure and storage facilities for processing and storing surplus milk after value addition.

2.1 Introduction

Each step in the food industry system e.g. food production, processing, transportation, storage, distribution and marketing, has some impact on the environment and there is much concern about environmental pollution. Due to the highly diversified nature of the food industry, various food processing, handling and packaging operations create wastes of different quality and quantity, which, if not treated, could lead to increasing disposal problems and severe pollution problems [24]. The preservation of the natural environment from industrial pollution is currently receiving much attention. In food industry, the most important problem is treatment and disposal of large quantities of waste water, a by-product of various processing operations. It is one of the largest industry groups comprising the manufacturing sector of the U.S. economy [47]. Likewise, India is the world’s second largest producer of food next to China, and has the potential of being the largest food processor. The total food production in India is likely to double in the next ten years [22]. Flour and grain; soft drinks and carbonated water; breweries; starch and miscellaneous food products; meat, poultry and fish; tea, coffee and other beverages; fruit juices; animal feed; sugar; distilleries and blending of spirits; cocoa, chocolates and sugar confectioneries; agricultural and food chemicals and industrial packaging are important sub-sectors of the food processing industry. Food processing projects involve the processing and packaging of meat products, fish and shell fish, dairy products, fruit and vegetables, grains and beverages production. It includes refinement, preservation, improvement of product, storage, handling, packaging and canning (Fig. 1). The processing may involve receiving and storing raw or partially processed plant, animal or other food materials, processing the raw materials into finished products, packaging and storing the finished products.

Food provides a sense of wellbeing and comfort and makes people feel good. In this world of a growing population, the need for affordable, safe and good quality food is rising constantly. On the other hand, there is growing awareness of customers who demand that food be produced and supplied hygienically in socially responsible and eco-friendly ways.

Acinetobacter baumannii is an opportunistic pathogen giving rise to nosocomial infections causing threat to public health. Studies of biofilm-forming genes was done in A. baumannii isolates by PCR technique. To study the relationships of genes a technique based on PCR sequences along with multilocus sequence typing (MLST) were utilized (Saeed et al., 2020). Goudarzi, Mehdi et al. (2019) studied the genetic diversity and biofilm formation analysis of Staphylococcus aureus causing urinary tract infections in Tehran, Iran. The biofilm forming and adhesion encoding genes were studied along with distribution of different agr and spa types in S. aureus isolates by PCR screening and multiplex PCR. The workers found that the ability to produce biofilm was higher among MRSA strains as compared to MSSA strains. The most prevalent biofilm forming gene was icaD (77.3%), followed by icaA (76%), icaB (57.3%) and icaC (50.7%). Acinetobacter baumannii is a nosocomial pathogen which is commonly present in hospitals and is a causal organism of many diseases. A. baumannii isolates have been known as multidrug-resistant (MDR). For controlling such serious organism only molecular techniques have been found to be effective. 

Indian Spices: These are dried, fragrant, aromatic or pungent vegetables or plant substances. They can be whole, broken or in ground forms. Primary function in food is seasoning rather than nutrition and which contributes to the flavour and piquancy in foods and beverages. These have been used as food additives widely since ancient times [http://www.imedpub.com/articles/herbal-medicines-possible-risks-andbenefits.pdf,https://www.ncbinlm.nih.gov/pmc/articles/PMC3170500/pdf/nihms307509.pdf). They also exert digestive stimulant action of food enhancing the taste. A few medicinal properties of spices are as being a tonic, carminative, stomachic, diuretic and antispasmodic (Aboaba et al., 2011; Achi, 2006 and Adefegha et al., 2012). This has led for pharmacological applications in the indigenous systems of medicine as digestive stimulants and to relieve digestive disorders and makes the food more palatable. In many countries around the world these are grown and reared for the purpose of trade and distribution. In the ancient times the spice merchants have been the most popular traders. India is well known for spices with a wide variety of recipes that are laced with them.

    1.    Control points are the points in the food processing chain where it is possible to control or remove hazards.

    2.    Critical control points are the control points in the processing chain where it is essential to a hazard, usually because there is no later step at which to establish control.

    3.    Preventative measures are the actions and activities needed to remove hazards or control them by reducing them to acceptable levels.

    4.    Critical limits for critical control points are measurements such as temperature and time, that must be met, or characteristics such as appearance and texture. Critical limits need to be validated.

    5.    Monitoring is the regular measurement or observation of a critical control point to ensure it is not beyond its critical limits.

    6.    Take corrective action to bring the process back under control before the problem (‘deviation’) leads to a safety hazard. Consider proper management (disposition) of any adversely affected product.

    7.    Verification procedures are tests and programmes that make sure the HACCP system working properly

    8.    Record keeping and documentation systems must meet the needs of the business and be adequate to show that the food safety programme is working.

Abstract

India has got a pool of medicinal herbs which are proved to be beneficial in curing various diseases. Their regular consumption not only will improve the health status but also reduce risk of lifestyle diseases. Though simple solutions, associated colloids, suspensions and emulsions are some of the traditional techniques for encapsulating functional agents, micro-encapsulation techniques is the most successfully employed technique for the controlled release of sensitive bioactive compounds. Owing to the targeted delivery of functional components directly to the human gut system, emulsion based delivery system are getting more attention now-a-days. With advancement in science, more complex and novel structures can be created which can successfully deliver these nutraceutical and bioactive ingredients into existing food systems. The technological interventions in emulsion science have opened great opportunities to improve the delivery of bioactive herbal components into milk and milk products. Various milk and milk products functionality can be improved in this way which will improve the gut- microflora within short period of time after consumption.

Incentives are a powerful stimulus to transfer of technology. They can encourage researchers to get their results to market, a new career path for many of them. They can create a culture that attracts and retains entrepreneurial staff, scientists, students and technology transfer professionals (TTPs) that view the university as a career path and a vehicle for making a contribution to society. Working in a pro-entrepreneurship team of colleagues can also lead to getting people to think in terms of making a connection between their work and its application. Incentives can be short-term or long-term in nature, and can be tailored to individual or collective requirements. As it is likely to be challenging for universities to design incentives that cater to every researcher’s or TTP’s desire, they can design incentive programs to cater to various aspects of human. Nature.
12.1 Why incentives matter in this ecosystem
Incentives can be either financial and non-financial. These are needed to ensure that researchers and TTPs remain motivated, aligned with institutional goals, and committed to the long-term process of technology transfer and commercialization. Incentive programs serve several important purposes:
• Motivation: Incentives energize individuals to pursue innovation and commercialization, often over long- and uncertain-time horizons.
• Retention: By offering recognition, rewards, and career advancement opportunities, institutions can retain high-performing researchers and skilled TTPs.
• Alignment: Well-designed incentives ensure that the personal goals of stakeholders align with institutional and societal objectives.

Industrial ice cream manufacturing is an integrated system involving food science, dairy technology, mechanical processing, refrigeration, quality assurance, hygiene, packaging, maintenance, utilities, manpower, and cold-chain management. This chapter explains how each manufacturing stage influences the next, from raw-material receiving and mix preparation through pasteurization, homogenization, aging, freezing, filling, hardening, storage, and distribution. It emphasizes process discipline, equipment reliability, trained personnel, KPI-based management, preventive maintenance, and cold-chain integrity. The chapter also addresses quality control, food safety, sustainability, digitalization, and operational challenges. It provides practical guidance for factory managers seeking consistent quality, productivity, controlled costs, reduced losses, and long-term operational excellence

Jamun (Syzygium cuminii L. Skeels) is an evergreen tropical tree which belongs to family ‘Myrtaceae’. It is commonly known as java plum, jambul, jamun, black plum, faux pistachier, Indian blackberry, doowet and jambolan. Jamun is native to the subtropical Himalayas, India, Sri Lanka, Malaysia and Australia. Its fruits are delicious and have great importance in folk medicine (Ayyanar and Subash, 2012).

INTRODUCTION

Now-a-days, food products with maximum health benefits and ease in preparation are gaining popularity globally, the reason being changing awareness level about food and dietary habits. Since ages, a number of methods like cooking, baking, drying etc. have been used to thermally process the food materials. Electrical sources of electromagnetic waves are very promising because of advances in nuclear and hydroelectric power as well as ongoing cost rises in traditional forms of fuel, of which there are only restricted reserves. The heating aids include use of wooden fuel, gaseous fuel, electric energy etc.; but the new era of electromagnetic rays had introduced new horizons of food processing by utilizing it in various unit operations (Sakai and Hanzawa 1994). Though, these radiations are gaining popularity in various walks of life; their destructive impact is much dominating. Hence, one should handle these radiations with utmost care as well as comprehensive understanding of the know-how and control over the process.

Introduction

Spices are botanical products of tropical and subtropical plants, mainly fruits, seeds, f lowers, roots, stems, leaves, bark, or whole plant (Golob & Nations, 1999). The dominant spices of trade including cardamom, cinnamon, cloves, ginger and pepper (A. Osman et al., 2019)including aroma, flavor, and color, to foods and beverages in an effort to enhance their palatability. Herbs and spices exhibit a plethora of medicinal properties including antimicrobial and antioxidant properties. Spices serve as natural preservatives to delay food spoilage and extend shelf life.

Probiotic Fermented food products
 
An individual requires adequate nutrients like proteins, carbohydrates, vitamins, and minerals to maintain diet. There has been an explosion of the consumer interest in the role of enhancing the health by some specific foods or physiologically active food components, so called functional foods (Hasler, 1998). The concept of functional foods evolved in the year 1990 in Japan.

Introduction
 
Developments in innovative food packaging technologies have evolved in response to the need for protection of the food product containing bioactive component from both external and internal environments and in response to consumer expectations for health claims, convenience and product safety. Consumers around the world are willing to pay extra for guaranteed product freshness delivered by the type of packaging. Many new packaging developments have focused on extending the shelf life of the product and on delivering a higher quality product to the consumer. These developments would not be possible without significant advances in the materials used in packaging and the incorporation of various types of sensors into food packaging. This chapter outlines the innovative packaing solutions in the production of functional food.

At present, food and nutrition security are the prime aspects which need to be addressed in an efficient way. It has been projected that by 2050 the global population will reach 9.2 billion which in turn enhances annual demand for agricultural products by 1.1 per cent from 2005 to 2050 (McKenzie and Williams 2015).

1. Introduction

Each day, millions of people take a small bag, drop it into a cup, pour in boiling water, and add a dash of milk plus a spoonful of sugar. People start the day with it, end the day with it, and also serve it socially or in times of distress (Herath and De Silva, 2011). The reason behind its popularity is based on the fact that the combination of theanine amino acid contained in tea and melatonin and B-complex vitamins contained in milk offers superior relaxing effects. Furthermore, the harmony of tea’s sweet aroma and milk’s natural sweetness will create a healthy low calorie drink.

Tea is one of the most pleasant drinks consumed by two third of the world’s population. It is not only the most popular and one of the oldest known beverages in the world but also an antioxidative agent available in everyday life which may help to prevent a wide variety of diseases such as cancers and heart diseases (Yang and Landau, 2000; Luczaj and Skrzydlewska, 2005). Tea provides a significant source of phenolic compounds in the diet. While polyphenols have long been of interest for their contribution to the astringenttaste of teas (Ding et al., 1992), research into the health benefits of tea has been actively pursued only in more recent times (Dufresne and Farnworth, 2001; Yamamoto et al., 1997). Even though the results are mixed for the relationship between tea and health in epidemiological and clinical studies, in vitro studies point to a range of mechanisms by which tea phenolics may help to offset chronic diseases such as cancer and cardiovascular diseases (Kris-Etherton and Keen, 2002).

Instructions for establishment registration and processing, filing for acidified and low-acid canned foods

Acid Food
 
A food that has a natural pH of 4.6 or below.
 
Acidified Food
 
A low-acid food to which acid(s) or acid food(s) are added and which has a finished equilibrium pH of 4.6 or below and a water activity (aw) greater than 0.85.
 
Authorized Company Representative
 
The person authorized by the company to sign the registration and process filing forms on its behalf. That person should possess the knowledge necessary to answer technical questions concerning filed processes.

Instrumentation and Advanced Analytical Techniques

    1.    Chromatography is defined as an analytical technique where by a mixture of chemicals may be separated by virtue of their differential affinities for two immiscible phases. One of these, the stationary phase, consists of a fixed bed of small particles with a large surface area, while the other, the mobile phase or “eluant”, is a fluid that moves constantly through, or over the surface of, the fixed phase.

    2.    The chromatogram evaluated qualitatively by determining the Rf or retention factor, for each of the eluted substances.

    3.    The Rf is a measure of that fraction of its total elution time that any compound spends in the mobile phase.

    4.    If the liquid phase is a polar substance (e.g. polyethylene glycol) and the mobile phase is non polar, the process is termed normal-phase chromatography

    5.    When the stationary phase is non polar (e.g. octadecylsilane) and the mobile phase is polar, the process is reversed-phase chromatography

    6.    The chromatographic separation of mixtures of large molecules such as proteins may be accomplished by a mechanism called size exclusion chromatography or gel permeation chromatography.

    7.    Separation of certain molecules is accomplished by a mechanism called affinity Chromatography in which specific binding between an antibody (stationary phase) and antigen (analyte) occur.

Introduction

Since long time fermentation process is in use for the production of functional food products for human welfare. SSF is a cost effective industrially important method that could be used by food industries to improve the nutritional profile of natural substrates (Salar and Purewal, 2016; Postemsky et al., 2017). The process is being carried out by trained microbiologists in the presence of aseptic conditions using suitable starter culture on steam sterilized substrates. Steam sterilized substrates possess minimal amount of moisture so as to support the microbial growth during the fermentation period. Growth of microbial strains on specific substrates during the fermentation time results in formation of koji (antioxidant rich fermented product). Selection of substrates and starter microbial strains is an important step in the fermentation process which will determine the nutritional properties of fermented product. One of the major advantages of SSF at industrial level is its potential to be utilized on cereal grains, fruits, vegetables and even agricultural wastes (Salar et al., 2012; Postemsky and Curvetto, 2015; Bhanja et al., 2009). Most common substrates that are being used as solid platform for the growth of microbial strains are cereal grains.

Introduction
The characteristics of bacteria used in the study and identification of bacteria depend upon the behavior of populations or cultures rather than of individual organisms. Nutrient materials provided in a form, suitable for growth, are known as culture media. A Growth Media or culture medium is a liquid or gel designed to support the growth of microorganisms or cells.
I. Types of Culture Media
Different types of culture media may be used for cultivation of microorganisms such as liquid, solid and semi-solid.
i) Liquid Culture Media: Includes nutrient broth, Glucose broth, litmus milk etc. these media are used for the preparation of large number of microorganisms, fermentation studies and various other tests.
ii) Solid Media: A little agar, gelatin or silica gel; is added to a liquid medium to impart a degree of firmness to it. Nutrient agar, Blood agar, etc. are examples of solid media that are used for developing surface colony growth of bacteria and mold.
iii) Agar Slant Media: Test tubes or small bottles containing about 5ml. of solid medium, e.g. nutrient agar, dissolved and allowed to cool in a sloping position.
iv) Stab Media: Tubes or bottles containing agar medium are allowed to solidify in the upright position.
v) Semi solid Media: These are more jelly like due to lower percentage of the solidifiers. These media are particularly used for determining motility of bacteria.

1. Sample: One or more units selected from a population of units, or a portion of material selected from a larger quantity of material
2. The Standard Operating Procedures (SOPs) should contain full working instructions as well as information on applicability, expected performance, internal quality control (performance verification) requirements and calculation of results. It should also contain information on any hazards arising from the method, from standards or from reagents.
3. Analytical Samples: The quantity of the analytical sample to be used is usually indicated in tests and assays. Unless otherwise specified, the quantity used may deviate by 10% from that stated. All quantitative determinations should be conducted on triplicate test portions and in these cases, the amount actually taken should be accurately weighed or measured and the result of the analysis calculated on this exact quantity. When substances are to be “accurately weighed” in a test or assay, the weighing is to be performed in such manner as to limit the error to ± 0.1% or less. Quantities smaller than 100 mg should be weighed to the nearest 0.1 mg.

Food preservation involves treating and safely handling food to prevent or delay spoilage while retaining colour, texture, flavour and nutritional value. Food deterioration may result from microbial growth, indigenous enzymes, oxidation, physical damage, insects, moisture, temperature and other environmental conditions. Bacteria, fungi and other microorganisms contaminate food through soil, water, air and biological sources. The effectiveness of preservation therefore depends on controlling intrinsic factors such as pH, water activity, nutrients and redox potential, together with extrinsic factors such as temperature, humidity and storage atmosphere. Understanding these mechanisms provides the scientific foundation for selecting safe and effective preservation methods.

Food safety and hygiene has been an age-old concern. However, today it has become an important aspect due to the rise in foodborne illnesses for the past few decades globally. It is estimated by WHO that annually 600 million suffer from foodborne diseases as a result of unsafe food consumption while 4,20,000 deaths are recorded globally.

Food Science is one of the ancient sciences and the genesis originates to the very beginning of human civilization. The advent of food science was associated with practice and observation by humans before establishment of scientific concepts. The ancient man knew that roasting or smoking of meat improves the edible quality and digestibility. As a consequence, he started practicing the same much before the reasons for the same could be established. Many of the modern preservation concepts had their origin in prehistoric period itself as humans could use techniques such as dehydration, fermentation and pickling to their advantage to improve the sensory attributes of the products and also to enhance their shelf life. Humans during the Pre-Neolithic period about 2,50,000 years before used fire for cooking meat. During the Paleolithic period humans not only roasted food but also developed grinding, pounding and drying. During the Mesolithic era, humans were desperate for food and got involved in extensive hunting and fishing. During the same time domestication of animals took place and animal husbandry became a reality (Stewart and Amerine 1982). The Neolithic period made qualitative changes in the food habits of humans and they changed from a food gathering and hunting society to one of food production (Heizer and Elsasser 1980).

Several centuries ago, perhaps as early as 6000-8000 BC, ancient man learned to domesticate species of animals for obtaining milk for their consumption. These included cows, buffaloes, sheep, goats, and camels, all of which are still used in various parts of the world for the production of milk for human use. The significance of milk as the most wholesome single food available in nature has never been debated. It is as ancient as mankind itself. The role and consumption pattern of milk in the traditional diet varies widely in different regions of the world. The tropical countries have not been traditional milk consumers, whereas in Europe (especially Scandinavia) and North America, milk and milk products have been traditional parts of the diet.

Veterinary public health deals with the contributions of veterinary medicine to public health. It is concerned, in the first instance, with the control and elimination of zoonoses and also with the following areas of activity:
    ·    Food hygiene (production, processing and distribution of foods of animal origin)
    ·    Environmental contamination and degradation by animal wastes and animal products
    ·    Comparative medicine (eg. Studies in animals on naturally appearing cancer, cardiovascular disease, metabolic and other disorders encountered in humans)
    ·    Laboratory animal medicine
    ·    Education and training in VPH and communication with the public
    ·    Reproduction biology
    ·    Aspects of mental and societal health
    ·    Emergency veterinary functions in natural disasters and other catastrophes.

Newer technologies involve fermentation by specific strains of lactic acid bacteria which bring specific fermentation under controlled conditions to a specific fermented product with enhanced organoleptic, nutritional and therapeutic qualities. Today health is of primary concern. It is important to have nutritionally balanced food with good bacteria. For this, probiotic foods top the list. The functional food concept was initially developed in Japan in 1990‘s as there were many side effects of allopathic medicines. A regulatory system has been initiated by the ministry of health and welfare to approve certain foods to improve health. These are now recognized as Foods for specialized health use (FOSHU). According to American dietic association (ADA) these foods are potential healthful food or food ingredients that may provide health benefits beyond the traditional nutrients. Probiotics are beneficial microorganisms. Probiotic foods as yogurt and sauerkraut are very well known. Fermentation increases the nutrient content and taste with the shelf life.

1.1 Importance of Food Process Engineering

Food occupies the first position among the hierarchal needs of human beings. It is more important than shelter and clothing. After production and be forec on sumpti on, food commodities are subjected to nume rous unfavourable physical, chemical, microbial or parasitic factors which may cause their spoilage or disease when consumed. To prevent such spoilage, a process engineer has to understand these and provide remedial measures. The two primary goals of processing operation are first to minimize post harvest losses and second value addition of raw food commodities through preservation and processing.

Present level of production of different types of food commodities and their estimated post-harvest losses along with monetary values are given in Table 1.1. It shows that various food items get lost, to the tune of 65 million tonnes, worth Rs. 76,000 crores per annum, during post-harvest phase. A substantial amount of these losses could be prevented if appropriate agro processing centers, having backward linkage with farmers to ensure constant supply of quality raw food materials, are established and operated. Profit generated through value addition must also be shared with farmers, who are only people in the world to create wealth every year, in the form of food, fiber and other commodities necessary for human survival. It can bring sea change in rural areas, where 65-70% population live, in respect of economy, health and happiness.

It sounds simple, but the above process is fairly complex and requires lot of coordination in order to succeed. The major challenge is to minimize the losses during the various post harvest operations and keep the food as fresh as possible. In order to do so, one must first try to understand the various causes of post harvest losses of food commodities and the various factors that influence theses causes. Secondly, we must use those food processing operations that will not only extend the shelf life of the produce but also convert the raw food commodities to more acceptable product to the consumer.

Human beings have been concerned about the concept of food safety since time immorial. Around the globe there has been major improvement in food safety management in the last twenty years. Looking into the progress made on the ways to make food safe and how to make them free from pathogens, this book, will explain food hygiene, the journey of research been taken in food safety till date and the challenges that we are going to face in future to ensure food safety and its wholesomeness. It also includes the role and responsibilities of the various sectors of society, namely governments, food industry, consumers and academia.

In the beginning of the twentieth century many modern food safety policies have been established. In the packing of meat and in food processing industries a lot of work has been done (Sinclair 1906). Another second generation policy includes the outbreak in the United States in early 1990s’ of pathogen E. coli O157:H7 and the BSE scandal of the late 1990s in the UK Lopman et al., (2003) have discussed on viral outbreak in europe. Currently, the crisis has come due to the economic and technological transformations which have taken place in food and the food supply system. As these changes have many health risks the public is very concerned. Therefore, the control forms of safety regulation and food safety laws have come into the picture. These policies include continuous line inspection, visual product inspection and detailed specification of approved hygiene practices. There is a need of a public health-focused policy which works towards the preventive integrated management of foodborne hazards from farm to table. This includes the use of modern science based risk management tools which will increase the efficiency accurately towards public health. A lot needs to be worked out for better implications. Current efforts to modernize food safety policy around the world are being worked out by the use of risk analysis and cost-benefit analysis in public administration and also the quality management in industry. The role of public economics plays an important role in food safety policies. For the social benefits the policy makers need to take decision on the costs and strengthening of the risk assessment. Today there are globalized markets and this can influence the health globally. Emerging economies like China are industrializing and urbanizing rapidly like that of Europe and North America.

The human body consists of trillions of cells and to be healthy it needs variety of foods and nutrients. The food around the globe includes a variety of spices which have many health benefits. It has been found that spices and vegetables in the diet are very important for living healthy. The traditional foods, nuts, seeds, wild fruits and vegetables which are used in the diet are grown in specific areas. Traditional food system plays a significant role towards maintaining the well-being and the health of people in any specific region.

There are many bacteria and fungi which produce biofilms. These are made up of living, reproducing microorganisms. These microbes form communities and adhere tightly to the surfaces and are resistant to many antibiotics. Many pathogenic microorganisms form biofilms which are difficult to be removed and are harmful.Such microbial colonies produce an extracellular polymeric substance (EPS) to protect them. The dental plaque was the first documented evidence of microbial biofilms. When such microorganisms get a moist environment they attach themselves to any object and reproduce forming an attachment by secreting a slimy, glue-like substance. It is possible with metals, plastics, natural materials, medical implants, kitchen counters, contact lenses, walls of a hot tub or swimming pool. Initially it was the investigation of many scientists to show the presence of biofilms

Beneficial effects of biofilms have also been shown (Rosche et al., 2009) especially in agriculture (Bogino et al., 2013; Berlanga and Guerrero, 2016) where it can be utilized as a biological control agents. They are used as biofertilizers to enhance crop production (Timmusk et al., 2017), hazardous pollutants can be removed (Irankhah et al., 2019). 

Indian Food Processing Industry (FPI) is one of the most untapped industries with abundant raw material to grow at a higher rate. FPI has been identified as a priority list in the “MAKE IN INDIA” program by the Government of India to boost its domestic manufacturing and export share. In the last few years, FPI has emerged as a high-growth and high-profit sector due to its immense potential for value addition while processing food products at various stages. The degree of processing of value addition to agricultural produce in India (20 %) is very low as compared to other countries, such as China (40 %), Brazil (70 %), and Malaysia (80 %). In India, the degree of processed food products ranges between 2 per cent (fruits and vegetables) and 35 per cent (milk) for various agriculture products. Hence there exist a huge potential for the growth of this sector in India. The export value of FPI was US$29.12 billion in 2016 and its share in world import was 2.18 per cent (Agarwal & Neogi, 2017).

India’s food processing sector covers fruit and vegetables; spices; meat and poultry; milk and milk products, alcoholic beverages, fisheries, plantation, grain processing, and other consumer product groups like confectionery, chocolates and cocoa products, soya-based products, mineral water, high protein foods, etc. The output of India’s food processing sector is expected to reach USD 535 Billion by 2025-26 (Invest India, 2020). Out of this, foreign investment is around Rs.10,000 crores (APEDA, 2020). Besides this, Government has also approved proposals for joint ventures; foreign collaboration, industrial licenses, and 100 per cent export-oriented units envisaging an investment.