eChapters

Dahi : It is the product obtained from pasteurized or boiled milk by souring, natural or by harmless lactic acid or other bacterial culture. It is also called Curd.
Daidzein : These are the substance from which daidzein is derived and are isoflavone C₁₅H₁₀O₄ found chiefly in legumes and esp. soybeans.
Dairy : It is a farm building often a single room in which milk is cooled and temporarily stored prior to collection for transportation to the commercial diary. Here the milk is treated and made into cream, butter and cheese. Or

Fermentation process is known to humans since ancient times. It involves the transformation of raw materials into value added or nutritional products as a result of microbial action or their growth. The fermentation as a science involves aspects of the biological action of microbes, biochemical changes in the substrates and metabolites formed and their effect on food, health and nutritional applications. While fermentation as technology, involves chemical engineering, food engineering and instrumentation aspects and commercialization of the products prepared keeping in view the consumer acceptability. The term fermentation is derived from the Latin word ‘Fervere - to boil’ thus describing the actions of yeasts on extracts of fruits or malted grain. Fermented foods and beverages enhance the pleasure of eating and their nutritional roles include indirect contributions through subjective enhancement of appetite. The instant mixes for fermented or fermentable products such as idli, dosai, dhokla and bhatura mix can reduce the overnight fermenting of the raw materials required for preparation of these products. The instant mixes for idli & dhokla are quite common in the market. However, instant mix for Jalebi and Bhatura are still emerging. Such products add convenience to the consumers and products can be prepared instantly. Certain other fermented foods include dairy products, pickles, sauerkrauts, Kanji, beverages, food supplements and nutraceuticals from fermented biomass.

Dahi: It is the product obtained from pasteurized or boiled milk by souring, natural or by harmless lactic acid or other bacterial culture. It is also called Curd (Plate 126).

Daidzein: These are the substance from which daidzein is derived and are isoflavone C15 H10 O4 found chiefly in legumes and esp. soybeans.

Dairy: It is a farm building often a single room in which milk is cooled and temporarily stored prior to collection for transportation to the commercial diary. Here the milk is treated and made into cream, butter and cheese. Or

Dairy: It is a farm building often a single room, in which the milk collected is pooled, temporarily stored prior to collection for retailing or direct sale to the consumer. OrDairy: It is a room, building, or establishment where milk is kept and butter / cheese is made or it is the department of farming / farm that is concerned with the production of milk, butter, and cheese oran establishment for the sale / and distribution of milk and milk products.

Dairy farm: It is a farm on which the principal activity is the milk production.

Dairy husbandry: It deals with care, breeding, feeding, milking of the diary cattle and the production and sale of the milk. It is the economical management of a dairy, including looking after the dairy cattle and proper disposal of milk and milk products.

Dairy products: These are the milk preparation, including butter, cream, cheese, ghee, yoghurt, pasteurized milk, tonned milk, butter milk etc processed and prepared in dairy. Ice cream and kulfi or sweets are not included in this.

Damage: Damage is a physical spoilage, often a partial deterioration, are subjectively judge and very difficult to measure and usually reported as % of the sample. Daminozide: It is a growth regulator which is used for red colour development in apple.

The upkeep of food processing equipment such as peelers, slicers, pulpers, and juicers are essential to ensure the smooth and efficient functioning of food production processes. These machines are commonly used in the food industry for preparing fruits, vegetables, and other raw materials by performing tasks such as peeling, slicing, pulping, and juicing. Proper operation of these machines enhances productivity, reduces processing time, and improves the consistency and quality of the end products. Efficient operation requires a comprehensive understanding of each equipment's mechanism and design, allowing operators to optimize the use of these machines while minimizing operational risks. For instance, peelers are designed to remove the skin of various fruits and vegetables without damaging the product, slicers are employed to cut products uniformly for further processing, pulpers are utilized to separate the pulp from seeds and skins, and juicers extract liquid from fruits and vegetables while retaining the flavor and nutritional content. However, ensuring smooth operation also depends on regular upkeeping practices. This includes routine inspections, lubrication, and cleaning to prevent wear and tear, mechanical failures, or contamination. Maintenance tasks also involve checking for malfunctions or parts that need replacement to avoid costly downtime. In addition, proper calibration and adjustment are crucial to ensuring the machines operate at their optimum efficiency and meet safety standards. Failure to maintain these machines can lead to inefficiencies, product defects, and even safety hazards. Therefore, understanding both the operational and maintenance procedures for peelers, slicers, pulpers, and juicers is critical for sustaining production standards, minimizing operational disruptions, and prolonging the life of the equipment.
PEELER
Peeling machines are very important in the food processing sector, providing efficient and accurate skin removal from various fruits and vegetables. These machines simplify the peeling procedure, ensuring that products meet strict standards for subsequent processing or packaging. This detailed guide focuses on the use of blade peeling machines, examining their features and uses in the food industry.

Traditional food-preservation methods reflect generations of practical knowledge concerning food safety, storage and seasonal availability. Although commonly performed at the household level, these methods are supported by scientific principles such as reducing water activity, lowering pH, inactivating enzymes and restricting microbial growth. Blanching, fermentation, sun drying, increasing solute concentration, pickling, smoking and curing preserve foods through different combinations of heat, dehydration, acidity, beneficial microorganisms and antimicrobial compounds. Their materials and equipment are generally accessible, making them suitable for home-scale application. The chapter explains the principle, mechanism, application and nutritional implications of each method while showing their continuing relevance in contemporary food systems.

Electromagnetic and advanced heating technologies offer rapid and energy-efficient alternatives to conventional food processing. These systems use microwaves, radio frequency, electrical resistance, infrared radiation, lasers, photonic energy and dielectric heating to generate heat within food or directly at its surface. Faster and more uniform energy transfer can improve microbial inactivation, shorten processing time and reduce damage to nutrients and sensory characteristics. The chapter explains the principles, mechanisms, applications and nutritional effects of microwave, radio-frequency, ohmic and infrared heating. It also considers laser-based and photonic techniques. Particular attention is given to heating uniformity, product properties, equipment control, energy efficiency and the prevention of underprocessed areas.

Emerging preservation technologies are being developed to meet increasing expectations for food safety, nutritional quality, sustainability and longer shelf life. Greater investigation remains necessary to understand their effects, limitations and suitability for large-scale industrial application. This chapter examines innovative physical, chemical and biological methods used at different stages of the food chain. Technologies include plasma-activated water, cold atmospheric plasma, advanced oxidation, electrolysed water, space-food preservation, hurdle systems and biopreservation. Their mechanisms involve reactive species, microbial membrane damage, oxidation, combined environmental stresses and beneficial biological agents. The chapter assesses their applications, advantages and disadvantages while highlighting research, regulation, process standardisation, equipment cost and commercial scalability.

INTRODUCTION

By the turn of the century, consumers have been more demanding, sophisticated and discretionary. They want much safer, higher quality, and more convenient to foods. The food processor need to get the best from their existing processing technology in addition to looking for newer ones. New and alternative food processing methods and/or novel combinations of existing methods are continually being investigated by the industry in pursuit of producing better quality foods more economically. Thermal processing is one of the most important food preservation techniques that was first introduced at the beginning of 1800 and has evolved itself in to one of the most scientific mature food processing techniques of recent times. Both science and technology have gone through tremendous transformation in the last two centuries and today it is the most reliable method to assure food safety and stability. Developments in thermal processing and evolution of non-thermal processing technologies like high pressure processing, pulsed electric field, pulsed light etc. have enhanced the utility of thermal processing principles and increased the awareness of possible alternative processing technologies.

INTRODUCTION

Now-a-days, food products with maximum health benefits and ease in preparation are gaining popularity globally, the reason being changing awareness level about food and dietary habits. Since ages, a number of methods like cooking, baking, drying etc. have been used to thermally process the food materials. Electrical sources of electromagnetic waves are very promising because of advances in nuclear and hydroelectric power as well as ongoing cost rises in traditional forms of fuel, of which there are only restricted reserves. The heating aids include use of wooden fuel, gaseous fuel, electric energy etc.; but the new era of electromagnetic rays had introduced new horizons of food processing by utilizing it in various unit operations (Sakai and Hanzawa 1994). Though, these radiations are gaining popularity in various walks of life; their destructive impact is much dominating. Hence, one should handle these radiations with utmost care as well as comprehensive understanding of the know-how and control over the process.

Introduction

Spray drying process has been used for decades to encapsulate food ingredients such as flavors, lipids, and carotenoids. During this drying process, the evaporation of solvent, that is most often water, is rapid and the entrapment of the interest compound occurs quasi-instantaneously. This required property imposes a strict screening of the encapsulating materials to be used in addition to an optimization of the operating conditions. Likewise, if the encapsulated compound is of hydrophobic nature, the stability of the feed emulsion before drying should also be considered.

In most countries there is no legislative definition of the term drawing a border line between conventional and functional foods is challenging even for nutrition and food experts. Functional foods do not have a specific place in food law; consequently there is a great deal of confusion about what they are, could or should be. A common perception is that functional foods are foodstuffs either with one or more specific added ingredients (components), or certain substances of common ingredients (components) removed and which are claimed to have a positive influence on human health or well being. The term ‘functional foods’ for this group of products is considered to be an unfortunate one since all foodstuffs are or should be functional and a more appropriate term might be helpful.

Instrumentation and Advanced Analytical Techniques

    1.    Chromatography is defined as an analytical technique where by a mixture of chemicals may be separated by virtue of their differential affinities for two immiscible phases. One of these, the stationary phase, consists of a fixed bed of small particles with a large surface area, while the other, the mobile phase or “eluant”, is a fluid that moves constantly through, or over the surface of, the fixed phase.

    2.    The chromatogram evaluated qualitatively by determining the Rf or retention factor, for each of the eluted substances.

    3.    The Rf is a measure of that fraction of its total elution time that any compound spends in the mobile phase.

    4.    If the liquid phase is a polar substance (e.g. polyethylene glycol) and the mobile phase is non polar, the process is termed normal-phase chromatography

    5.    When the stationary phase is non polar (e.g. octadecylsilane) and the mobile phase is polar, the process is reversed-phase chromatography

    6.    The chromatographic separation of mixtures of large molecules such as proteins may be accomplished by a mechanism called size exclusion chromatography or gel permeation chromatography.

    7.    Separation of certain molecules is accomplished by a mechanism called affinity Chromatography in which specific binding between an antibody (stationary phase) and antigen (analyte) occur.

1. Introduction

Dairy industry size and demand precede all other segments of food industry. Reporting milk production of 146.3 million tonnes in 2014-15, India is currently occupying first position in world scenario (NDDB, 2015). In spite of limited shelf-life, milk offers various opportunities of product diversification, processing optimisation and convenient packaging solutions. All categories of dairy products are unique and vary in market value. The operating margins in value-added dairy products are almost twice of liquid milk business due to reduced need of refrigeration and added shelf-life. The appeal and satiety it offers makes them ambrosia for consumers. Baked and fried dairy products (BFDP) are those class of dairy products in which main processing operation is either baking or frying. In addition to preservation of milk solids for longer time at room temperature, manufacture of baked and fried dairy products add value to milk and also provide considerable employment opportunity (Pal and Raju, 2007;Patil, 2011). The characteristic flavour and colour development of the product rely on this unit operation or cooking method and it cannot be replaced. The unique difference in baked and fried dairy products is that in baking dough is prepared and for frying batter is prepared which vary significantly in characteristics (Table 1). The quality of the finished product greatly depends on how the dough or batter is handled.

1. Introduction

Product development is a basic activity in the food industry. Each company has a product mix, often including hundreds of products, which are constantly evolving - old products dying, products reaching maturity, products contributing to rapid growth and new products being introduced.

In the food industry, just as any other industry, product and process development is considered a vital part – indeed the lifeblood – of smart business strategy. Failure to develop new and improved products relegates firms to competing solely on price which favours the players with access to the lowest cost inputs (land, labour etc). Product and process development (commonly referred to as product development) is systematic, commercially oriented research to develop products and processes satisfying a known or suspected consumer need. Product development is a method of industrial research in its own right. There are essentially four basic stages in the model product development process.

Starter cultures were unknown until 1878, when Lister isolated pure cultures of the lactic acid bacteria responsible for milk acidification. In the 1980s, improvements to standardization and ripening of butter were made by introducing pure starter cultures. During the period 1910-20, Orla-Jensen, von Freudenreich, Sherman, Hammer and other dairy microbiologists prepared pure cultures for fermented milks. Commercial production and use of starter cultures grew rapidly and was widespread at the beginning of the 20^th century.

Dairy starters are the ‘heart’ of fermented milk products, the most crucial component in the manufacture of high-quality fermented milks. The cultures are harmless food-grade microorganisms, active bacteria intentionally grown in milk or whey or other formulated media which impart desirable and predictable flavour and texture to fermented dairy products.

The dairy microorganisms employed in milk fermentation are single-strain or multiple-strain cultures of lactic acid bacteria, producing different types of fermented milk products. Usually, one or two strains dominate the milk environment. Individual strains can be selected in advance for their resistance to both bacteriophages and antibiotics. Mixed starter cultures are used to ensure that fermentation will continue after a bacteriophage attack. Bacteriophages are highly strain-specific, and if the dominant strain in a mixed-strain starter culture succumbs to an attack, the phage-resistant mutant or the next dominant culture maintains a satisfactory rate of lactic acid production.

Cleaning and sanitizing of dairy plants is essential for providing safe milk products to the consumers as well as for ensuring the good shelf life of these products. It ensures cleanliness, hygiene of the equipments and surroundings and also ensures that the products meet the safety standards. Cleaning of dairy plant consists of broadly of removing the soil from the surface of the equipment by dissolving or suspending the soil in a warm solution of suitable cleaning agents. In the dairy industry, the soil is primarily milk and milk product residues which may be more or less modified by processing treatment. 
Milk and milk product residues are complex mixtures of milk sugar, fat, proteins and mineral salts. Some of these solids are present in emulsion, some are in a colloidal state, and others are in true solution. When milk products are heated in vats or pumped through sanitary pipe lines or over coolers, the metal or glass surfaces become coated with milk remains, which, if allowed to dry, may be difficult to remove.

Abstract

Seaweeds are the large marine algae that grow almost exclusively in the shallow waters at the edge of the oceans. Seaweeds are rich sources of many bioactive compounds and nutraceuticals. These factors are considered under two categories: Seaweed phytochemicals and Seaweed polysaccharides. They are rich in soluble dietary fibers, proteins, minerals, vitamins, antioxidants, phytochemicals, and polyunsaturated fatty acids, with low caloric value. The red, brown and green seaweeds have been shown to have therapeutic properties for health and disease management. Usually they are only used as gelling agent and stabilizers in the food and pharmaceutical industries, but current research has revealed their potential medicinal uses against many diseases like cancer, allergy, diabetes, oxidative stress, inflammation, thrombosis, obesity, hypertensive and other degenerative ailments. In line with other natural foods like fruits, vegetables and grains, there has been marked interest within the scientific community to assess which fractions of seaweeds may be linked to the historically observed health benefits. As consumer health and nutrition become more influential in the food industry, the use of seaweed as an ingredient is on the rise in product development. For several centuries there has been a traditional use of seaweeds as food in China, Japan and the Republic of Korea. This review undertakes successful exploration of potential of seaweed gums as an functional ingredient in a number of food applications. This addition has the potential to enhance the nutritional quality of a product and hence human health.

3.0. Introduction

A hazard is any factor that may be present in the food or food products and can cause harm to the consumer either through injury or illness. Hazards may be biological, chemical and physical. It may be a biological, chemical or physical property or condition of food with the potential to cause an adverse health effect. Therefore, these hazards can be grouped into three broad categories (Fig 3.1). Further, various stages during which processing hazard can takes place in a food industry is illustrated through Fig. 3.2. Effect of different potential hazards on human health along with symptoms is presented in Annexure IV.

Introduction

The world does not function without mathematics. In our day-today life we come across many problems which are solved mathematically. The mathematical logical system follows “Yes” or “No”, “True” or “False”, “1” or “0” which are precise outputs for the precise inputs. There are several ambiguous or vague problems which cannot be given with accurate precise value with the conventional logical operators. Beyond this Boolean logic operator the logical system which mimics the human brain and gives precise solutions for the imprecise linguistic variables is the Fuzzy logic system. Fuzzy logic is a novel approach that intends to imitate human reasoning using calculations and operations with fuzzy systems and linguistic variables. The problems with vague imprecise data which we come across in our day today life cannot be solved by conventional binary logical method. The fuzzy logic solution spontaneously splits a complex problem into its component parts. This reduces the solution to its component parts. Compilers may re-assemble the code for effective execution on some target, but at the programmer level the problem is a number of simple tasks.

Fuzzy sets theory was introduced by Zadehin the year 1965, and it allows uncertain phenomena to be treated mathematically.

Introduction

Biological, physical, chemical and other hazards associated with food have been detailed in Chapter 4. This chapter would address the risks associated with these hazards as well the scientific approach is assessing them. Risk analysis is an inevitable platform for food safety assurance which measures the impact of hazards on the health of consumers. To improve public health and maintain consumer trust in the food supply and to provide a sound regulatory foundation for national and international trade in food, an efficient food safety management system is essential. Therefore, World Trade Organization (WTO) developed food regulations governing international trade in foods based on science and risk assessment. A sound knowledge of food hazards and risks they pose to consumers, along with the capacity to take appropriate interventions, deserve top priority of a responsible authority and industry.

Risk

The word ‘risk’ is used to describe a situation that involves a possibility of something undesired to happen (Rowe, 1977). In food processing, hazards are considered as risks. Hazards are often thought of as synonyms of risks, however, hazards are quite different from risks.

A food borne hazard is defined by Codex as “a biological, chemical or physical agent in or condition of food, with the potential to cause an adverse health effect”. A risk is the chance, low or high, that any hazard will actually cause harm. Some of the emerging food hazards that must be assessed and managed are listed in table 1. Assessment and management of risks posed by these hazards is the responsibility to be urgently addressed by the scientific community.

Street Foods-An Introduction

The National Policy on Urban Street Vendors (2004) defines street vendor as a person who offers goods for sale to the public without having a permanent built-up structure but with a temporary static structure or mobile stall (or head load). Street vendors may be stationary occupying space on the pavements or other public/private areas, or may be mobile in the sense that they move from place to place carrying their wares on push carts or cycles or baskets on their heads, or may sell their wares in moving trains and buses. The total number of street vendors in India has been estimated to be ten million and the number of street food vendors has increased sharply in the past few years (Bhowmik 2005).

The preparation and sale of street food is an age-old activity with historical roots and complex socio-economic and cultural implications. Street food vendors are mostly outside the regulation of the governments and unregistered thus making them an informal sector. Similar to most other informal sector enterprises street food operations too are carried out with minimal investment, infrastructure and as familial operations. Street food outlets are seen mushrooming at crowded public places like bus stands, cinema halls, market areas and parks. They operate as temporary structures or as pushcarts parked near the roadside or at pavements. Most street food vendors operate their food stands alone and are able to generate sufficient income for sustenance.

Introduction

Food quality control systems in all sectors of food chain around the globe are driven by guidelines to help the authorities to enforce rules and regulations to protect the public from fraud and deception in various levels of food production and facilitate fair trade. This also helps authorities to select suitable options of infrastructure and enforcement mechanics. Such documentation provide possible infrastructure and approaches to national food control systems to use them or further improve them wherever needed (Temesgen, 2015). To make a food control system most effective; it is strengthened by improving the existing fragmented legislation, multiple jurisdictions, and weaknesses in surveillance, monitoring and enforcement in many countries. Organisations like FAO and WHO help enforce food laws and regulations to meet a country’s sanitary as well as phytosanitary requirements.

Generally, food law may be divided in two parts: (i) a basic food act, and (ii) regulations. The Act itself give broad principles, while regulations contain detailed provisions governing the different categories of products coming under the jurisdiction of each set of regulations. Detailed provisions are needed for effective administration of and enlightened compliance with the basic food laws. In governments where there is a division between the responsibilities of the legislative and executive branches, the legislative branch enacts the basic law, while detailed regulations are elaborated and promulgated by the executive agency or agencies responsible for administering the law. Regular revisions of regulation is necessary because of novel scientific knowledge, change of processing technology, or quick action to protect public health. Such revisions can be made much more expeditiously by executive agencies than by legislative bodies. In some of the countries food standards are a part of the regulations; in the other countries they are separate enactments.

To look into the nutritional security around the globe, FAO and WHO, have undertaken the analysis of policies. They have covered many issues which include; World Summit on Food Security, pillars of food security and availability, access, utilization, stability, effects of food insecurity, stunting and chronic nutritional deficiencies, depression, anxiety and sleep disorders. There are many challenges to achieve food security. These include global water crisis, land degradation, climate change, agricultural diseases, food versus fuel, politics, food sovereignty, food wastes, risks to food security, population growth, fossil fuel dependence, homogeneity in the global food supply, price setting, land use change, global catastrophic risks, agricultural subsidies and role towards children.

Indian Spices: These are dried, fragrant, aromatic or pungent vegetables or plant substances. They can be whole, broken or in ground forms. Primary function in food is seasoning rather than nutrition and which contributes to the flavour and piquancy in foods and beverages. These have been used as food additives widely since ancient times [http://www.imedpub.com/articles/herbal-medicines-possible-risks-andbenefits.pdf,https://www.ncbinlm.nih.gov/pmc/articles/PMC3170500/pdf/nihms307509.pdf). They also exert digestive stimulant action of food enhancing the taste. A few medicinal properties of spices are as being a tonic, carminative, stomachic, diuretic and antispasmodic (Aboaba et al., 2011; Achi, 2006 and Adefegha et al., 2012). This has led for pharmacological applications in the indigenous systems of medicine as digestive stimulants and to relieve digestive disorders and makes the food more palatable. In many countries around the world these are grown and reared for the purpose of trade and distribution. In the ancient times the spice merchants have been the most popular traders. India is well known for spices with a wide variety of recipes that are laced with them.

Introduction

Fermentation is one of the oldest food preservation techniques that can be traced back thousands of years. Fermentation is referred as one of method of biopreservation as it leads to extending the shelf life of foods that involve the use of microorganisms and their metabolites. (Ross et al., 2002) For millennia, the fermentation of food by using micro organisms has been used as a technique for ensuring shelf life extension and improving the quality characteristics, texture and flavour of food products as well as to develop new innovative products. The major bacteria used in food fermentation worldwide are lactic acid bacteria (LAB). LAB is a multitude of genera including Lactobacillus, Lactococcus, Streptococcus, Leuconostoc, Pediococcus, and Enterococcus. Lactic acid bacteria constitutes a diverse group of bacteria that enjoys the status of “generally regarded as safe” (GRAS) and “qualified presumption of safety” (QPS) by the Food and Drug Administration (FDA) and European Food Safety Authority (EFSA), respectively. In this respect, starter microorganisms are known to produce a wide array of antimicrobial and protein-acoustic compounds to inhibit the growth of undesirable flora in foodstuffs resulting in shelf life extension. (Hill et al., 2017).

Introduction

One of the main properties of fermented foods whether alkaline or acidic is the presence and activity of beneficial organisms which themselves make these foods functional. These functional micro organisms have varied roles like conversion of the native constituents of raw materials to forms in which they are more bioavailable, formation of taste, colour and flavouring compounds thereby imparting pleasantness to foods, removal of anti-nutritive factors in food by converting them into much less harmful compounds or degrade certain native toxic elements present in food, formation of biopreservatives which will extend the shelf life of foods and provide microbial security, as pathogens and spoilage microorganisms fail to readily grow in such hostile conditions. These functional microorganisms also produce in foods certain health-promoting bioactive compounds like enzymes, vitamins, minerals or increase the digestibility which may not have been in the original food or raw material before fermentation. In other words they improve the nutrition profile of the foods or naturally fortify foods. Some of these beneficial effects may also lead to the formation of certain compounds which show antioxidant properties in foods. These very roles played by these functional micro-organisms form criteria in their selection for use in food fermentations when they are intentionally added to foods both to initiate and accomplish desired fermentations.

Introduction

Over the past few decades, probiotics have become the most promising and emerging field in human nutrition and health across the world including India and other developing countries. The global profit generated from the probiotic market is expected to reach approximately 12,753.4 million US$ by the end of 2026 (Probiotic market report, 2018). The factor fueling the demand for probiotics is the growing awareness among consumers about their health benefits. The association of probiotics with health benefits stretches back to a time before microbes were discovered. However, the scientific evidence of the health benefits of probiotic microorganisms on the wellbeing of the host was firstly proposed by Russian Nobel laureate Ellie Metchnikoff in 1905. He reported that consumption of fermented milk containing Lactobacillus spp. is responsible for the healthy and long life of Bulgarian people (Anukam & Reid, 2007). Afterward, several of scientific progressions were made to reach the current status where probiotics are not only the subject of food and medical research but also the source of a multi-billion dollar global industry.

The term probiotic is derived from the Greek words, which means ‘for life’. According to the Food and Agriculture Association of the United Nations (FAO) and World Health Organization (WHO), probiotics are defined as “live microorganisms which when administered in adequate amount promote the health of the host” (Joint, FAO, 2002). In 2014, International Scientific Association for Probiotics and Prebiotics (IPPA) did minor correction in the definition given by FAO-WHO and defined them as “Live microorganisms that, when administered in adequate amounts, confer a health benefit on the host”

Introduction

Increasing knowledge regarding the impact of diet on human health along with state-of-the-art technologies has led to significant nutritional discoveries, product innovations, and mass production on an unprecedented scale. In particular, naturally occurring bioactive extracts or single compounds thereof, that are believed to benefit human health, have spawned an important and dynamic new area of research resulting in substantial advances in nutritional knowledge. There is also growing awareness that dietary source and form of food may affect overall health. Suitably, the role of food as an agent for improving health has been recognized, initiating the development of new classes of food, known as functional foods (Honkanen 2009).

The concept of functional foods is to improve the general conditions of the body and decrease the risk of illness and disease. That is to say, bioactive compounds present as natural constituents or as fortificants in food having the potential to provide health benefits beyond the basic nutritional value of the product. Marine-derived nutrients and other marine bioactive components have excellent potential as functional food ingredients as they possess advantageous physiological effects, with medicinal characteristics and added health benefits such as anticancer or anti-inflammatory activity.

The marine world, due to its phenomenal biodiversity, is a rich natural resource of many biologically active compounds such as polyunsaturated fatty acids (PUFAs), sterols, proteins, polysaccharides, antioxidants and pigments. Many marine organisms live in complex habitats exposed to extreme conditions and, in adapting to new environmental surroundings, they produce a wide variety of secondary (biologically active) metabolites which cannot be found in other organisms. Moreover, considering its great taxonomic diversity, investigations related to the search of new bioactive compounds from the marine environment can be seen as an almost unlimited field.

Introduction

In recent years, food scientists, nutritionists and health experts have become more inclined towards functional food components present in various food sources due to their positive influence on health, which is beyond providing the basic nutrition.Nowa days, generalconsumers are also seeking healthier food options because of nutritive food habits and awareness. Therefore, it has triggered the studyof food and deep mining of food components from nutritional and health benefit’s perspective also. Functional food ingredient can be defined as any componentwhich is more than a source of nutrient i.e. provides additional specific health benefits. Milk is well known to exert positive effect on human health, provides nutrition and widely consumed all across the globe in form of either milk or milk products. As per the definition, milk is heterogeneous blend of lacteal secretion which contains various elements that displays wide range of chemical and functional activities. It has been more than a source nutrition and supplement to different age groups either neonates, youngsters or grown up peoples (Park, 2009). It is one of the basicelements of many diets either in its native form or fermented form which every class of people can afford and had evolved as one of the important nourishment sources. Milk in its native form contains fewer bioactive components while fermentation process produces numerous secondary metabolites which possess various health-beneficial properties (Stanton et al., 2005).

The fungi are usually:

a) Parasites or saprophytes b) Lithophytes

c) Epiphytes d) Insectivorous

Food preservation methods are important in order to avoid glut or shortage of food in the market. In addition it helps to increase the shelf life, maintain the nutritional and sensorial quality, facilitate transportation and improve the overall value of the commodity. Even though various methods of food preservation are available now. Most of the conventional methods are thermal preservation methods which may result in the loss of volatile nutrients and flavour, different sensorial modifications, microbial spread with or without gas production, damage to the microstructure, rupture of coating or packaging surfaces and microbial recontamination caused by non-hermetic closure.

Growing population has increased the demand on food supply. Providing safe and hygienic food to the population is an essential consideration. Food safety systems and tools need to be in place to assure safe food, until which the food businesses would not flourish or be able to build their reputation. Food safety systems and management tools are important as they assist food business organizations to build confidence in people regarding food safety.

Abstract

Water is very essential nutrient and total of only 3% pure water is present on earth. Some natural, anthropogenic and human activities discharge wastes, containing heavy metals into the different water resources and ultimately destroying the ecosystem. Numbers of methods are used for water purification like conventional (includes physical and chemical) and biological. Conventional methods are expensive, generate large quantities of sludge and inefficient at low concentrations of metal. Biological method includes biosorption and bioaccumulation, are ecofriendly and economic and have advantages over conventional methods. Biosorbent material includes microbial biomass, agro-wastes, and industrial by-products are used to treat heavy metal from liquid samples. Actually these biosorbent have number of functional groups on their surfaces which help in binding of metal from aqueous solution. In this article, biosorption of zinc, arsenic and copper are described through lactic acid bacteria that are not harmful, nonsporulating, fastidious, fermentative and generally regarded as safe. Many of these species are probiotic and producing exopolysaccharides and biofilms. It has great resistance to survive in gut environment and efficiency to biosorb metal from gut. It has some significant characteristics that represent a useful tool for decontamination of food and beverages from heavy metals. Biosorption mechanism and factors behind the process make these strains competent of removing these heavy metals.

Abstract

Bioinformatics is an interdisciplinary field which plays a major role in various disciplines including drug discovery, pharmacogenomics, proteomics, comparative genomics, microbial genome applications in biotechnology. Food plays a vital role in various processes in body and also helps in controlling various chronic diseases. Food safety and food processing are very crucial factor for human lives. Bioinformatics has been successfully applied in food industry for improving various factors of food such as flavor, taste, allergenicity, probiotics, optimizing the quantitative and compositional parameters for food processing and assessing the food safety. In this chapter, we have given an overview about the role of bioinformatics in food industry.

(Ref: 1997-2016 American Meat Science Association)
The methods used for cooking meat can be divided into two groups:
1. Dry heat methods
2. Moist heat methods

1. Introduction

Freezing is a method of food preservation that involves lowering the temperature below the freezing point of food generally -18 °C or below. During freezing of food the water present in them changes its state to form ice crystals. Human beings in the colder regions have used freezing as a means of preservation since they learnt hunting and gathering foods. The foundation of the modern frozen industry was laid in mid 1920 when Clarence Frank Birdseye, an American inventor used cold brine solution to chill packaged fish and extended it further for quick freezing of meat poultry fruit and vegetables. 

1. Introduction

Drying is a thermo physical and physicochemical process, which involves removal of excess water from a solid, semi-solid or liquid food material by evaporating it into vapour phase on applying heat, to reduce growth of microorganism or to completely eliminate their growth. Hence, reducing the water activity increases shelf life of food products. Drying medium for carrying evaporated moisture can be air or steam. Benefits of drying include increase in shelf life, easy to transport, handle food material and reduce packaging cost as it reduces bulk weight. 

1. Introduction

Heat transfer from a hot surface is a general method or technique of heating liquids and slurry type of food materials. In such cases the heat can be generated either directly by a heating element or indirectly from a hot medium like steam or hot water via a heat exchanger (e.g. shell and tube, plate). Therefore, for the heat transfer during heating by these techniques a temperature gradient will be required, which usually causes the fouling on the surface of the heating element or heat exchanger. Afterwards the fouling not only reduces the heat transfer rate but also results in quality deterioration within the product which is being processed. 

1. Introduction

Thermal processing treatments are the conventional methods that have been broadly employed in the food industry to guarantee the microbiological safety of food materials at a minimal cost. However, these thermal treatments cause undesirable changes in flavor, nutritive value, texture, or color of the finished product that impact food quality (Pan et al., 2017). Growing the modern consumer’s demand towards the minimally processed and “fresh-like” taste foods has challenged the food industry to develop novel non-thermal preservation techniques as an alternative to thermal processing. 

1. Introduction

In recent years, consumers have been demanding convenient, healthy and safe foods which have ’fresh-like’ characteristics with a long shelf-life. These demands include important food quality such as appearance, texture, taste and nutritional content which are strongly impacted by the way it is processed. Another major concern among the consumers is food safety and quality. The shelf-life of the food products are mostly influenced due to the action of various harmful microorganisms and enzymes. The conventional food processing technologies, such as thermal treatments (pasteurisation and high temperature sterilisation), evaporation, drying, frying and baking can assure the microbiological safety and/or stability of processed food products, but affects the food quality and nutrients (heat sensitive vitamins, flavour and other bioactive compounds) (Zhang et al., 2019)

Introduction

Fruits and vegetables are an important part of healthy human diet. Consumption of vegetables is widely promoted by Governmental health agencies since it provide consumers essential nutrients such as vitamins, minerals, dietary fiber and phytochemical compounds, at a relatively low calorie density. Furthermore, the consumption of vegetables has been strongly associated with reduced chronic and degenerative diseases such as cancer and heart related ailments. The United States Department of Agriculture recommends five to nine servings of fruits and vegetables daily (Berger et al 2010). In recent years, fresh-cut produce has become more popular due to their convenience, freshness, taste and assumed “Healthiness”.

1. Introduction

Food is an essential requirement for survival. We work hard to earn money so that we can feed ourselves. However, at the end of the day, many of us are unsure about the quality of food we consume. The allure of money, along with a general disregard for humanity, has resulted in the addition of adulterants to food ranging from simple stones in rice to the more harmful chemicals in food of daily use. Surprisingly, food fraud is not a modern issue. There are several references to fraudulent food behaviour as early as the Romans, but it is usually considered to be the Victorian who places food fraud on the map.

In recent years dietary supplements have gained attractiveness within fitness and wellness circles. The other commonly used terms areNutraceuticals, dietary supplements or wellness-focussed foods. Being highly nutritious they are associated with a number of potential therapeutic effects. Nutrient-rich ingredients like fruits, vegetables, nuts, seeds, and grains are often considered dietary supplements as well. Oats fibre called, beta glucan, which has been shown to reduce inflammation, enhance immune function, and perk up heart health.

1. Conventional and Non-conventional Dietary Supplements

Nutrient-rich foods like fruits, vegetables, and legumes are often considered dietary supplements. Meanwhile, modified foods have been fortified with micro and macro nutrients to boost health benefits of foods.

MAC: It stands for modified atmospheric coating.

Macaroni wheat: It is tetraploid wheat and is preferred for making macaroni, vermicelli etc.

Macaroni: Pasta made from semolina and shaped in the form of slender tubes (Plate 230).

Macaroon: A small cookie composed chiefly of egg whites, sugar, and ground almonds or coconut (Plate 231).

Mace: An aromatic spice consisting of the dried external fibrous covering of a nutmeg (Plate 232).

Mach number: It is a number representing the ratio of the speed of a body (flow velocity) to the speed of sound (sonic velocity) in a surrounding medium (as air).

Macro economics: Studies of economics in terms of whole systems esp. with reference to general levels of output and income and to the inter-relations among sectors of the economy.

T A Spoilage: The spoilage of low and medium acid canned foods by Clostridium thermosacharolyticum which breaks sugars and form acid and gas (moisture of H2 and CO2 ) resulted in swelling and in severe cases even bursting of can may occur and the spoiled food usually has a sour odour.

T cell: It is one of the lymphocytes (helper T cell) that differentiate in the thymus, possess highly specific cell-surface antigen receptors, and include some that control the initiation or suppression of cell-mediated and humoral immunity (regulation of T and B cell maturation and proliferation) and others that lyses antigen-bearing cells. It is also called T lymphocyte.

Table cream: It is also called coffee cream, single cream or homogenized cream. It is used in coffee and tests about 20% butter fat.

Table oil expeller: It is a type of the machine that is used for extraction of the oil from the oil seeds (Plate 313).

Table ready meat: Cooked and smoked sausages are called table ready meats.

Table wine: Table wine is a dry wine with low alcohol content. Dessert wines certified sweet wine.

For a researcher, laboratory is another home where he/she spends much time every day. As we take care of cleanliness, safety, and design of our home, we must take care on these aspects of laboratory too. It is the responsibility of the laboratory personnel to ensure cleanliness/safety of the laboratory. Since every laboratory chemical and equipment have the potential to cause harms if not used with appropriate precautions. Hence, as a laboratory user, it is our responsibility to follow the basic safety/biosafety rules and regulations set up by the Principal Investigator of the laboratory, Institutional Biosafety Committee, as well as by other regulatory authorities, if any. We must be aware of the general/personal protective measures, cleanliness and laboratory waste disposal prior to joining a research laboratory. In this chapter we will briefly discuss about personal safety, chemical safety, cleanliness, waste disposal, etc.

A. Personal Safety

1. Personal Protective Equipment (PPE)

Personal protective equipment (PPE) is special arrangement to protect the worker from specific hazards because of using a hazardous substance. PPE does not reduce or eliminate the hazard, but protects the wearer from exposure to hazardous material. PPE should not be reused or brought outside the laboratory as these might increase the risk of spread hazardous material or contamination. Used PPE must be kept inside the laboratory in a designated waste storage area adjacent to the work area.

The term probiotic means “for life” and it is currently used to refer bacteria that are associated with beneficial effects for humans and animals. The credit for first observation made on the positive role of some selected bacteria was attributed to Eli Metchnikoff, the Russian born Nobel Prize recipient, worked at the Pasteur Institute in the beginning of the last century. Metchnikoff suggested that - “the dependence of the intestinal microbes on the food makes it possible to adopt measures to modify the microflora in our bodies and to replace the harmful microbes by useful microbes”. Interim Henry Tissier, a French paediatrician, observed that children with diarrhea in their stools had a low number of bacteria which are characterized by a peculiar Y shaped morphology. On the contrary, these “bifid” bacteria were abundant in healthy children. Based on these observations, Tissier suggested that “bifid” bacteria could be administered to patients with diarrhoea to help restore a healthy gut microflora. These two scientists were the first to give scientific intimation for the use of probiotics when even the term probiotics was not coined. Later in 1965, the term probiotics is coined by Lilly and Stillwell to describe substances produced by one microorganism, that stimulate the growth of another. Subsequently, Parker defined probiotics as organisms and substances that contribute to intestinal balance. Further, Fuller (1989) improved the Parker’s definition of probiotics as “live microbial feed supplements which beneficially affect the host animal by improving its intestinal microbial balance”. Havenaar and Huis in’t Veld (1992) extended the probiotic definition with the description: “A viable mono or mixed culture of microorganisms which, applied to animal or man, beneficially affects the host by improving the properties of the indigenous microflora”. At the end of the millennium, Salminen and his coworkers (1999) proposed the following definition: “probiotics are microbial cell preparations or components of microbial cells that have a beneficial effect on the health & well- being of host”. Whereas FAO/WHO (2001) defined probiotics as “living bacteria that, when administered in adequate amounts, confer a health benefit on the host”. This definition for probiotics is widely accepted.

Introduction

Lactic acid bacteria (LAB) are characterized as Gram-positive cocci or rods, non-aerobic but aerotolerant, able to ferment carbohydrates for production of energy and lactic acid. Lactic acid bacteria includes different major genera: Lactococcus, Enterococcus, Lactosphaera, euconostoc, Melissococcus, Oenococcus, Pediococcus, Streptococcus, Tetragenococcus, Vagococcus and Weissella. Other genera are Aerococcus, Microbacterium, Propionibacterium and Bifidobacterium. Through carbohydrate fermentation along with lactic acid, various organic acids are produced due to which there is a fall in pH level and creates unfavorable environment or inhibits growth of some undesired microorganisms. The low pH makes organic acids lipo -soluble, allowing them to invade into cytoplasm of pathogens by rupturing cell membrane. LAB are generally regarded as safe (GRAS), and have a significant role in the preservation of foods and fermented products. Under controlled conditions they can be used as natural competitive microbiota or as specific starter cultures. Antagonistic substance like bacteriocins are produced from lactic acid bacteria that inhibits the activity and growth of other bacteria and these bacteriocins are active against various pathogens in minimum concentration. These are antimicrobial peptides modified by post -translational mechanisms and exported to the extracellular medium. Till date about 239 bacteriocin producing LAB genome and 789 putative bacteriocin producing genes cluster are stored in the public databases. Many gram positive and gram negative bacteria in the environment produce bacteriocins.

The GOI is pushing to strengthen infrastructure for the production of quality milk, as well as for procurement, processing, and marketing of milk and milk products through the following Dairy Development Schemes:

6.1. Dairy Entrepreneurship Development Scheme (DEDS)

Dairy Entrepreneurship Development Scheme (DEDS) was started in September 2010. This scheme is being implemented through NABARD which provides financial assistance to commercially bankable projects with loans from Commercial, Cooperative, Urban and Rural banks.

Objective of the scheme

To generate self-employment and provide infrastructure for the dairy sector, to set up modern dairy farms and infrastructure for the production of clean milk, to encourage heifer calf rearing for conservation and development of good breeding stock, to bring structural changes in the unorganized sector so that initial processing of milk can be taken up at the village level, to upgrade traditional technology to handle milk on a commercial scale, to provide value addition to milk through processing and production of milk products.