eChapters

Introduction
Milk reception at the processing plant is a crucial stage in the dairy supply chain, as it serves as the first point of quality control and the gateway to further processing. The success of milk processing hinges on the careful handling, evaluation, and preparation of the raw milk upon arrival. The stringent protocols at reception ensure that only high-quality raw milk enters the system. By implementing advanced technology, strict hygienic standards, and efficient handling practices, processing plants can maintain milk’s safety and quality, setting the foundation for the production of superior quality dairy products. The intricate procedures at this stage safeguard consumers' trust and ensure compliance with regulatory requirements.
Milk is transported to dairy plants either in cans or in road/rail tankers. The designated area within the dairy plant where milk is first received, inspected, and graded for acceptance is referred to as the milk reception dock, platform, or raw milk receiving dock (RMRD). This reception dock is specifically designed and equipped to ensure the efficient handling of milk, enabling its swift unloading while also accommodating the cleaning and sterilization of used containers.
Since the quality of milk is a critical factor for its subsequent processing, the decision to accept or reject milk must be made promptly upon its arrival, following a thorough evaluation. The milk reception process should be meticulously planned, and the equipment must be selected to ensure efficient intake operations, particularly in facilities handling large milk volumes. Any delay can result in deterioration of milk before unloading, increased labour requirements, and higher operating costs. To avoid such issues, milk deliveries should adhere to a strict schedule, and the reception process should be completed within a stipulated time frame of time. This ensures seamless continuity of the dairy plant operations.

Performance metrics and indicators
Traditionally, in the past, TTOs’ performance was measured in monetary terms, such as universities’ revenues derived through commercialization. The methodology, however, is prone to shortcomings, in light of the fact that various TTOs do not receive significant amounts of financial proceeds through their technology transfers. In addition, relying on income does not depict a TTO’s true potential, in light of the fact that it does not account for the total range of outputs of research and commercialization opportunities. Recognizing these limitations, academics have found innovative ways to measure TTO efficiency, such as monetary and non-monetary indicators. Financial indicators typically entail measurable outcomes such as number of signed licenses, income from licenses, royalties collected, equity income, and number of spin-offs or startups created. These are actual gauges of the economic returns from commercialization efforts, and in the majority of instances are used in the context of benchmarking the performance of a TTO in a comparative context, perhaps against peer institutions or industry averages. Non-financial indicators consist of royalty distributions to academics, having an integral role in encouraging academics to participate in commercialisation activity. By incorporating monetary and non-financial metrics, universities might have an enhanced, comprehensive evaluation system for their TTOs. Such evaluation is sure to ensure assessment extends beyond short-run monetary metrics and looks to incorporate several organization, institutional, and research-oriented determinants in driving successful technology transfer.

Analytical testing provides objective evidence of the composition, safety, physical structure, and sensory quality of ice cream. This chapter introduces the major analytical approaches used in industrial ice cream manufacturing, including chemical, physical, microbiological, and sensory evaluation. Important parameters such as fat, milk solids-not-fat, protein, sugars, ash, acidity, pH, overrun, meltdown, fat destabilization, texture, and product weight are discussed. The chapter explains how different tests support routine quality control, product development, troubleshooting, research, regulatory compliance, and shelf-life studies. It emphasizes that no single analytical parameter can define overall quality. Effective product evaluation requires integrated interpretation of chemical, physical, sensory, microbiological, and storage data.

Introduction

The economic choice of any process is the criterion for efficient utilization of the by-product that is produced along side of main product. During manufacture of major dairy products, the dairy industry also ends up in producing different by-products. Various by-products of dairy industry are skim milk, butter milk , whey and ghee residue. Dairy industry is particularly confronted with the problem of economic utilization of whey and ghee residue. The techno-economical problems associated with utilization of these by-products, is now receiving considerable attention. More efficient, cost effective and sophisticated technologies have opened up new vistas for manufacture of number of derived by-products. Further more the market for dairy by-products, as an ingredients in other consumer food products like snacks, ready meals and microwavable food products, has been growing.

Over the past several years, there has been significant change in consumer perception on quality and food safety. Consumers are demanding healthy, nutritious and cost-effective products processed under controlled hygienic conditions. Food safety concern, in recent years, has become dominant and important. The requirement of food safety and quality assurance has never been so demanding as it is now. Globally food safety is being addressed independent of food quality. In anticipation of consumer demands on food safety, food sanitary standards at national and international levels are under constant review. Several inter governmental agencies/organizations are engaged in work related to quality assurance and food safety. Amongst these World Health Organization (WHO) and Codex Alimentarius Commission (CAC) play the major role in protecting public health and defining standards of quality and safety aspects of foods for regional and or world wide usage.

INTRODUCTION

Drying or dehydration is defined as the removal of moisture from its initial level to a level which renders food materials safe for subsequent storage. It is a simultaneous heat and mass transfer operation in which heat is supplied externally to evaporate moisture and air is forced through the bed to carry away the moisture. Drying is the oldest technique to preserve food materials. Advantages of drying include reduction in water activity, enhancement of shelf life, maintenance of flavor and nutritive value, and reduction in packaging and transportation costs. When the water activity of the dried product is reduced to below 0.6, most microorganisms do not grow and the rates of other deteriorative reactions are substantially reduced.

INTRODUCTION

Food being the basic need of all humans and other living organisms, the production and processing of food is a continuous activity with several new procedures and technologies adoption; thereby the quality of food needs an emphasis which also protects the consumers by providing safe and suitable food. The contaminated foods, food borne illness outbreaks though at times referred to poor consumer handling, commitment for provision of safe food to consumers is also a Governments concern. In the recent years, food safety measures through Food Safety programmes and increase in awareness are being followed. The food production in our country is the most promising sector which is the cause for white revolution and green revolution. India ranks first in the world in production of cereals and milk and second largest producers of fruits and vegetables. India stands second in inland fish production. It is second largest exporter of rice and so on. Food processing sector has a great potential which covers a wide spectrum of products encompassing agriculture, horticulture, plantation, animal husbandry and fisheries. But the growth of food processing industries in our country is rather slow. However, to encourage, the government is providing lot of incentives in the recent years.

MAC : It stands for modified atmospheric coating.

Macaroni : Pasta made from semolina and shaped in the form of slender tubes.

Macaroni wheat : It is tetraploid wheat and is preferred for making macaroni, vermicelli etc.

Macaroon : A small cookie composed chiefly of egg whites, sugar, and ground almonds or coconut.

Mace : An aromatic spice consisting of the dried external fibrous covering of a nutmeg.

T A Spoilage : The spoilage of low and medium acid canned foods by Clostridium thermosacharolyticum which breaks sugars and form acid and gas (moisture of H₂ and CO₂) resulted in swelling and in severe cases even bursting of can may occur and the spoiled food usually has a sour odour.

Introduction

The term probiotic is derived from Greek and means ‘for life’ as opposed to antibiotics which means ‘against life’. The history of probiotics began with the consumption of fermented foods which was first observed in ancient Greeks and Romans [1]. The term, probiotic, as is used today was first used by Parker in 1974. Parker defined ‘probiotic’ as substances and organisms which contribute to intestinal microbial balance. In 1989, the term was modified further by Fuller. Thus, probiotic is a live microbial supplement which affects the host’s health positively by improving its intestinal microbial balance. This definition was broadened to include mono or mixed culture of live microorganisms which benefits animals or man by improving the properties of the indigenous microflora [2]. Some authors have interpreted probiotics as microbial cell preparations or components of microbial cells that have a beneficial effect on the health and well-being of the host.

Introduction
 
In recent years, there has been a growing interest in the functional foods because they can provide physiological benefits additional to nutritional and energetic, as for instance, antihypertensive, antioxidant or anti-inflammatory [1]. A functional food can be defined as a food that produces a beneficial effect in one or more physiological functions, increases the welfare and/or decreases the risk of suffering a particular disease. Furthermore, new types of products, derived from food, called nutraceuticals have recently been developed. These products, usually employed as food supplements, are marketed as tablets and pills, and can provide important health benefits.

1. Introduction

The development of food products using composite flour has increased and is attracting much attention from researchers, especially in the production of bakery products and pastries. This article focuses on the use of composite flour to produce food products, namely bread, biscuits, and pasta, with looks at on its impact, following some improvements made, on the sensory quality, rheology characteristics, and nutritional values as well as its overall acceptance. The blending of wheat flour with various sources of tubers, legumes, cereals and fruit flour in different percentages to produce variety of food products are also reported. The positive effects of the use of composite flour can be seen in the final product related to the functional and physicochemical properties and health benefits of raw blended flour along with percentage blending. Overall, composite flour is a good new approach to utilizing uncommon food products as the application of composite flour produced products with different characteristics and quality, depending on the types and percentage of wheat flour used in the formulation.

Bread has been the staple food worldwide and habit of bread is derived in part due to the convenience and wholesomeness. Wheat is a commonly used cereal for making bread as it gives the desired texture due to the presence of gluten forming proteins like gliadins and glutenins (Decock & Cappelle, 2005). In earlier times idea of composite flour in developing countries originated in order to cut down on import of wheat (as it was not grown in many countries) but in recent past refined wheat flour consumption has lead to health issues and thus in order to improve nutritional and functional properties composite flour has been the norm. Composite flour is the one obtained by mixing wheat flour with cereal and legume flours for making bread and biscuits (Dendy, 1992). However, mixing of non-wheat flours, roots and tubers, legumes or other raw materials can also be regarded as composite flour.

1. Introduction

Now a days, foods are not intended to only satisfy hunger and to provide necessary nutrients but also to prevent nutrition-related diseases and improve physical and mental well-being of the consumers (Menrad, 2003). In this regard, functional foods play an important role. The concept of functional foods which has evolved as the role of food in the maintenance of health and well-being and in the prevention of disease, has received increased scientific and commercial interest is recent past. Growth of functional foods markets include increased consumer health awareness, increased popularity of healthy foods, search for food-based drug substitutes, self-medication approach promoted by busy lifestyles and rapidly developing health conscious markets. Functional foods can be defined as foods “that can beneficially affect one or more target functions in the body, beyond adequate nutritional effect, in a way relevant to an improved state of health and wellbeing and/or reduction of risk of disease” (Stanton et al., 2005).

1. Introduction

A confection is sweet tasting delicacies developed using sugar and other carbohydrates with optionally selected chocolate, nuts, fruits, vegetables or gums. Depending on the formulation, the confection may vary from sugar boiled hard lozenges to gum based soft gummy toffees, from chewing gums to mouth melting chocolates. The versatile and broad variation in the product created by differentiating flavours, ingredients and processing has greatly influenced the market demand of these products. Although confection is non-essential commodity but still relished by people of all economic strata and hence creating a great market space and scope of new products.

In confectionery research and development area, current popular sector is functional confections. The sector has been on the trends radar globally, accounting for 8% of the global confectionery market, and most accepted and successful in Japan.

Importance of Traditional Foods

Traditional convenience food, or tertiary processed food, is commercially prepared food designed for ease of consumption. Products designated as convenience foods are often prepared food stuffs that can be sold as hot, ready-to-eat dishes; as room-temperature, shelf-stable products; or as refrigerated or frozen products that require minimal preparation (typically just heating).

These products are often sold in portion controlled, single serve packaging designed for portability for “on-the-go” eating. Convenience food can include products such as candy; beverages such as soft drinks, juices and milk; fast food; nuts, fruits and vegetables in fresh or preserved states; processed meats and cheeses; and canned products such as soups and pasta dishes.

Traditional Foods

Preparation of Vadagam and Appalam

Vadagam is a traditional product mostly prepared from rice flour and also sago. It is an deep fat fried product and consumed as a side dish in the meals. Papad also known as appalam or papadam in South India, is essentially a thin wafer like product, usually circular in shape rolled from a dough made out of pulse, cereals, edible vegetable oil, alkaline and mucilaginous additives. It is normally consumed in toasted or fried form. In India, papad has been associated and deeply entwined with social customs/ rituals and has been developed into culinary art.

Packaging means a coordinated system of preparation of goods for distribution, storage, merchandizing and shipment at optimum cost compatible with the requirements of the product. It also includes the art, science and technique used, preparatory to and during transportations and selling of goods and technical methods and work processes related to the above preparations.

The broad range of dairy products necessitates that packaging equipment and materials encompass nearly all types from flexible to rigid and from high to low barrier. The general types of packaging and equipment are discussed below with a focus on fluid products but it must be recognized that other dairy products such as cheese have their own particular packaging requirements and technologies.

Role of packaging in distribution and sale: The essence of what makes milk and dairy products important source of human nutrition also causes rapid deterioration and high perishability. The major role of dairy packaging is to retard the deterioration by preventing microbial recontamination and excessive chemical deterioration. Fluid products are especially susceptible to microbial, enzymatic and chemical degradation. In addition to retarding quality loss, packaging must provide containment, facilitate use, identify products, communicate, and appeal to consumers, all with maximum production efficiency. The large volumes and low unit value means that these functions must be met with minimal economic cost and environmental impact. Because food packaging is generally highly regulated, legal requirements must also be understood and met.

3.1 Introduction

The production of food grains in India is around 200 million tonnes and most of the grains are converted to primary products after primary processing such as threshing, dehulling, de-branning. Some of the grains are converted to secondary products such as flour or grits. The yields of primary and secondary products range between 65-80% indicating a wastage of 20-35%. These wastes lead to unclean surroundings and air pollution due to fine dust. Hence, proper waste management/utilization of food grain processing industries has become a societal need for a clean environment.

The term ‘waste’ has different meanings to different people. It can be retractions from the main process or less utilizable by-products or undesirable waste material depending on the commodity. All food-processing industries generate wastes and create problems for its management. The best way the food processing industry can treat its waste is to avoid producing it. Much of the waste now being produced or generated in some food processing plants can become a managed resource for producing economic credits. In fact, waste utilization is one of the world’s prominent industries and its activities cut across the normal divisions into which industries are placed. Similarly, there will be wastes from agriculture, animal and meat industries, textile, rubber etc. Even the local governmental activities that collect and dispose the household wastes become apparent in terms of the huge size of it.

Abstract

Upcoming era of functional foods has led food scientists and food processors to deeply understand the role of phytochemicals in promoting human health. Phytochemicals are the secondary metabolites produced by plants and are commonly found in our daily food. Natural food products seem to be good substitute for pharmaceutical supplements to improve health status of people. Therefore, consumer's interest is growing in the food items naturally containing phytochemicals, each having there own functional and health aspects. Most discussed phytochemicals in the present paper are carotenoids such as lycopene, beta-carotene, lutein and, polyphenols such as phenolic acids, flavonoids, anthocyanins and isoflavones. Major dietary source for carotenoids include orange and yellow fruits and vegetables as well as green leafy vegetables whereas polyphenols are found abundantly in green tea, wine, berries, cocoa, broccoli. Carotenoids can also be used as colorants in foods during processing. The role of polyphenols as an antioxidant is well characterized because of there ability to scavenge free radicals or bind pro-oxidant metal ions by means of their hydroxyl group. Carotenoids also play an important role in protection against photooxidative processes by acting as singlet molecular oxygen as well as peroxyl radical scavengers, thus acting as potent antioxidant. Apart from antioxidative properties multitude of health benefits are associated with carotenoids and polyphenols such as reduced risk of age-related macular degeneration, certain cancers such as prostate, lung and colon, prevention of cardiovascular disease and enhancing immunity towards infection. Hence, continuous developments are being made to combine phytochemicals to avoid improper combinations that may nullify the effect of supplements and cause lower absorbance.

Abstract

Probiosis has emerged in the early 20^th century after Ellie Metchnikoff proposed his hypothesis that organisms were reponsible for longivity of ethnic people of Balkan region of Russia and ever since it has grow into a major scientific field which has its applications extending into farming, aquaculture and prophylactics in humans. Vast research was carried out on this topic and Lactobacillus sp. and Bifidobacterium sp, are the two important genus that are highly studied and widely recognized universally as conventional probiotics. Apart from these two genus there exists many other bacterial species which have shown promising probiotic potential. Such non conventional probiotics are widely used in some parts of world as commercial food products with health beneficial effects, pharmaceutical products or being tested for their safe utilization in human beings. The present chapter tries to covers some genera and their potential and application in prophylactics.

Introduction

Packaging helps to maintain the benefits of food processing and enables safe transportation of foods from their point of origin to point of consumption without compromising the wholesomeness of processed food in terms of its physico chemical and microbiological quality. However, packaging technology must balance protection effects with environmental issues, material and energy costs and should comply regulations on municipal solid waste disposal and release of pollutants. These regulations are different in jurisdictions around the world.

Safety implications of food packaging raise concern in recent times due to advances in technology leading to variety of packaging materials and additives. Also there is an alarming situation of damages to environment and natural resources of the planet, due to indiscriminate use and disposal of packaging materials.

However, food packaging as a means to protect and preserve food integrity, to enhance its shelf life and improve consumer appeal cannot be overlooked.The applications of food packaging and its primary role to protect and preserve food has remained the central focus of packaging innovations.Packaging materials are available in variety of forms and are made up of variety of ingredients.While paper. While paper, plastic, petroleum based materials have been in use for many years, the recent approach is towards environment friendly options from agricultural produce such as plant fibres sugars and starches. Advances in packaging has led to development of newer materials and combinations with specific economic and functional benefits.Food packaging ,in general , provides the ability to withstand thermal processing and acts as a barrier against light, moisture,oxygen and other sources of contamination.

The growing chemicalization of milk presents a great challenge to the dairy industry. A number of chemical compounds get entry into milk, intentionally or un-intentionally, through their direct or indirect usage for crop, animal; or dairy husbandry practices, as environmental contamination. Several of these contaminants may be naturally present on grazing grass or use as pesticides for green fodder or they may get entry through use of compounded animal feeds. These eventually get excreted into milk as residues and pose problems to food safety. The risk of health hazards arises from veterinary drugs, residual pesticides, heavy metals, detergents and micotoxins etc. It is therefore, become necessary to detect these contaminants in order to make food safe for human consumption.

Detection of Aflatoxin M 1 in Milk

Material requirements : Glass column(30 cm x 1 cm), Silica gel (63-200 µg particle size), volumetric flasks, beakers, micro pipette, HPLC equipment fitted with C 18 column(25 cm x 4.6 mm) and fluorescence detector.

Reagents: Sodium chloride solution (400g in 1000ml water), acetonitrile/water mixture (25:75), sodium sulphsate, chloroform, toluene/acetic acid mixture (9:1), acetonitrile/diethyle ether/hexane mixture (1:3:6), chloroform/acetone mixture (8:2), n-hexane and aflatoxin standard ampule.

Preparation of stock solution : Transfer contents from 2.5ml or 0.5ml ampoule, quantitatively, to 25ml or 50ml volumetric flask respectively. Rinse ampoule with small quantities of chloroform and collect rinsing into flask. Makeup the volume with chloroform, mix well and store at 5º C or below. These solutions will contain 1 µg per ml aflatoxin M1.

Working solution : Transfer 0.5ml of stock solution to a 10ml conical flask. Evaporate contents to dryness using inert gas. Dissolve residue in 10ml of acetonitrile/water mixture. Prepare series of dilutions containing 0.0025, 0.005, 0.01 and 0.025 µg/ml aflatoxin. Similarly treat 5ml of stock solution to give a concentration of 0.5 µg/ml.

Introduction

For human beings, the three most important necessities for their survival are food, clothing and shelter. among all of these food is the top most priority. Food safety and Standards defines food into 8 categories (Nutraceutical, Heath Supplements/Dietary Supplements, Food for special medical Purpose, food for Special Dietary Use, Novel Food, and Functional food) in regulation, 2016. A growing trend of health products, concept of food as medicine, associated public interest and consumers demand leads to a progressive research interest in functional foods and nutraceuticals development. Abnormal lifestyle, food habits (consumption of junk food) and low physical activity creates an exponential growth in a number of chronic diseases; obesity, cardio-vascular syndromes, osteoporosis, arthritis and cancer (Das et al., 2012) among the developing and developed countries. While nutraceuticals and functional foods are becoming an alternative affordable system to reduce the risk of disease through prevention in spite of high tech therapies and costly medicines.

The current survey showed that worldwide revenue of functional foods is projected to increase from about 300 billion U.S. dollars in 2017 to over 440 billion dollars in 2022. Also according to the International Food Information Council (IFIC), health benefits achieved from functional food components may provide benefits far beyond than basic nutrition. Products like dietary supplements containing vitamins, functional foods such as sports drinks or products fortified with probiotic play a major role in the market rise (www.statista.com).Indian and Chinese traditional foods, recipes, spices and diversified ingredient market is well known around the world from centuries. Hence, a double-digit growth rate is also experienced in Asian nutraceutical and functional food market. Another reason for the growing trend in consuming functional foods is education and proper health awareness. Furthermore, people are more concern about the optimization of health-promoting capabilities in their diet using dietary supplement and fortified foods. While, food product based industries are marketing health supplements using scientific facts and the relationship between diet and health. Government, non-government organizations (NGOs) and web-based online information also play a key role in promoting and trending food based health products.

Introduction

Military personnel’s are deployed for operation in adverse climatic conditions like desert, cold (arctic) and high altitude regions. Stressful and challenging living conditions have affected the outcome of many battles, campaigns and wars. Acclimatization to the environmental condition is a very essential stage of deployment. The time for acclimatization is restricted/limited for the effective operation towards the purpose of deployment. The performance of the military personnel in various deployed regions depends on the nutrition provided by the food consumed. The operational rations are designed to provide the essential nutrition to enhance their performance during training and/or operational situations.

Nutrition requirement of military personnel is different from that of the civilians. The nutrition requirement also varies with the kind (sedentary or continuous work) and region (plain, desert or high altitude) of deployment. The environmental stress caused by extreme heat, cold, etc. also influences the nutrient requirement/ intake. The insufficient dietary nutrient intake affects the immune function, pace of recovery from illness and injury and also the physical performance (Tharion et al., 2005).

The operational ration is also designed in such a way that it provides to the military personnel the required nutrition from the build in nutrition reserves during certain operational situations. Considering the vital criteria for nutritional requirement, the menu for the military personnel is designed. The chapter discusses the past, present and future of the army operational ration (AOR) in various countries of the world and the influence of advances in food processing methods in AOR menu.

Introduction

Since the last century, nutritional science has made a remarkable contribution to the biological sciences through identification of diverse nutrients and their sources, functions, requirement, deficiency symptoms and their remedial measures etc. The advent of high-throughput sequencing technologies together with bioinformatic tools identified the presence of 100 trillion of microorganism belonging to all forms of life in the gastrointestinal tract. In fact, the whole gut microbial consortia are considered as a virtual organ in human and animals and is responsible for the development and functioning of series of essential physiological activities including digestion, absorption, metabolism, immunity, homeostasis, brain and gut development, resistance against disease etc. According to the modern scientific dogma, the human being is in fact a “Superorganism”, carrying consortium of numerous symbiotic Eukarya, Bacteria, Archaea and Viruses. The gut microflora has been co-evolved with human and presumed to maintain an intricate balance among different microbial communities. Upon introspection about the functionality of the gut microflora, it is amply clear that a few of these microorganisms have the ability to make healthy state; while other members are responsible for health-related problems including food poisoning, diarrhoea, abdominal pain, indigestion, inflammatory bowel disease, predisposition to colon cancer, compromising immunity, allergy etc.

Abstract

The natural color of foods is due primarily to carotenoids, anthocyanins, betanin and chlorophylls, either as inherent food constituents or as food or feed additives. These compounds have drawn considerable attention in recent years, not because of their coloring properties, but due to their potential health-promoting effects. Their occurrence and levels in foods, along with the factors that influence the composition, have been widely investigated. Processing effects have been actively studied. In spite of the intense search for plant and microbial sources and efforts to increase yield, few natural food color additives have reached the market. Developing new colors for the food industry is challenging, as colorants need to be compatible with a food flavors, safety, and nutritional value, and which ultimately have a minimal impact on the price of the product. In addition, food colorants should preferably be natural rather than synthetic compounds. Micro-organisms already produce industrially useful natural colorants such as carotenoids and anthocyanins. Microbial food colorants can be produced at scale at relatively low costs. This chapter highlights the significance of color in the food industry, why there is a need to shift to natural food colors compared to synthetic ones and how using microbial pigments as food colorants, instead of colors from other natural sources, is a preferable option.

Safe Food means

• It is not injurious to health
• It does not contain any filthy, rotten, decomposed or diseased animal
• substance or vegetable substance
• It is produced undermost hygienic conditions to produce/manufacture it, inferior or cheaper ingredients/raw materials are not used
• Food additives/processing aids used are permitted and are within prescribed limits.
• It is not misbranded

1. Introduction

Law of conservation of mass says that material cannot be created nor destroyed, which is the basic fundamentals of physical science. Many engineering analyses involve material balances. These balances are simply accounting processes that account for all material in processes of interest. The material balance is one of the most useful tools fundamental in the “engineering approach” to design new process. The law of conservation of mass tells that the total mass of all material entering any process must equal the total mass of materials leaving plus the mass of any material accumulated in the process.

1. Introduction

Mixing is a fundamental and widely practiced operation in many fields, such as chemical, pharmaceutical, and food processing industries. The mixture’s primary aim is to increase the homogeneity of the material in bulk (Uhl and Gray, 1986; Patterson et al., 2004). Mixing means the random distribution of two or more ingredients throughout a system, which differs from the term’ agitation,’ which is defined as establishing a particular flow pattern with the liquid, especially in a circulatory motion within a container. Mixing is a necessary step in the context of food processing operations. 

Introduction

In the ancient era, adulteration was practiced as economical fraud like the addition of water to milk and stone to grains and pulses but nowadays it has become a global concern due to adverse health effects of adulterants on a human being. Words like substitution and admixture can help define adulteration. In general, adulteration is defined as either removal of valuable constituents from food or intentionally/ unintentionally addition of substandard quality material to degrade food quality. Food adulteration not only negatively affects the quality of food products but also results in numerous adverse health effects along with significant economic problems (Cheng et al., 2015).

Introduction

The act of substituting food or drink with low grade quality ingredients thereby the f inal product will fail to the meet the regulation or standards and in most cases even unfit for human consumption (Augustin et al., 2009). With demand for profit based manufacturing market, there will be increase in points of adulteration with cheaper alternatives or inferior grade ingredients in the production of food or drink (Banti, 2020)]. Adulteration is highly prevails in developing countries such as India, where about 70% of milk supply is adulterated on day to day basis (Bittar et al., 2017). It is every nation and each individual’s responsibility to provide safe and nutritious food to the consumers.

Introduction

Spices are botanical products of tropical and subtropical plants, mainly fruits, seeds, f lowers, roots, stems, leaves, bark, or whole plant (Golob & Nations, 1999). The dominant spices of trade including cardamom, cinnamon, cloves, ginger and pepper (A. Osman et al., 2019)including aroma, flavor, and color, to foods and beverages in an effort to enhance their palatability. Herbs and spices exhibit a plethora of medicinal properties including antimicrobial and antioxidant properties. Spices serve as natural preservatives to delay food spoilage and extend shelf life.

Introduction

Food fraud is not new it can be traced back to ancient Rome and Athens, where regulations prohibiting the adulteration of beverages with flavours and colours were in place. It exists since the food trade has been started. Food fraud affects all food supply chains, thus the entire food sector. Due to globalisation and the increasing complexity of supply lines, food supply chains are becoming more vulnerable to food fraud (Robson et al., 2021a). A fraud is “any act or omission relating to the use or presentation of false, incorrect, or incomplete statements or documents, or non disclosure of information in violation of a specific obligation, and any other illegal activity affecting the financial interests” (EU No. 2017/1371).

A variety of glassware like burettes, pipettes, measuring cylinders, flasks, separating funnel etc. are used for experimental purposes in a laboratory. All of these need to be used and handled correctly, otherwise the chances of experimental error and damage to the glassware increase significantly. Being made of glass, they need special care while washing and using in experiments because even a minor mistake in handling of glassware may result in breakage of the apparatus. With this precautionary note, let us discuss about some of the specific glassware used in the laboratories.

Burette

Burette is a long graduated glass tube with a stopcock at one end for delivering a known/desired volume of a liquid, especially in titration experiments. It contains a stopcock at the lower end and a tapered capillary tube as the stopcock’s outlet. The flow rate of liquid from the tub stopcock’s outlet can be controlled with the help of stopcock valve. Burette is used for measuring the volume of a solution/chemical added in a quantitative (titrimetric) estimation experiment.

Procedure of using a Burette

The burette reading must be noted before and after delivering the chemical/ solution from the burette. The difference in these two readings is the volume of the chemical/solution released out of the burette. The solution should be released slowly/drop-by-drop to avoid any experimental error/mistake. If the solution/chemical is allowed to pour out too fast, the wall of the burette will not drain properly and some liquid may remain sticking to the surface of the walls. Moreover, excessive amount of chemical may be released/dropped during the experiment, particularly during the titration experiment wherein we need to know the exact amount of acid/base needed to get neutralization point. Thus, any minor mistake may lead to get a faulty reading. The measuring capacity of a burette usually used in a laboratory is 50 mL.

Bovine milk contains an average of 3.5% protein. The concentration of protein changes significantly during lactation. The first few days of lactation exhibit the main change in protein concentration. The main changes occur in whey protein fraction.

Distribution of Protein in Milk

80% casein and 20% whey protein (β lactoglobulin- 10%, α- lactalbumin- 5%, serum albumin, and immunoglobulin- 5%).

Fractionation of Milk Proteins Fractionation of milk protein is mainly done by acidification at pH 4.6 around 30 °C.

Almost 80% of the bovine milk protein precipitates at pH4.6, and this fraction yielded by precipitation is called casein.

The remaining soluble part of the protein is referred to as whey protein/serum protein/ non- casein protein. Wide variation is observed in the casein- whey protein ratio in different types of milk(protein synthesis is independent of animal diet)

Significant Difference Between Casein and Whey Protein

1. Casein precipitates at pH 4.6 but does not precipitate whey protein at that pH. This property of milk protein is commercially exploited for the preparation of industrial casein and certain varieties of cheese.

2. Chymosin or other proteolytic enzymes produce specific changes in casein resulting in coagulation in the presence of calcium. Whey protein does not undergo this type of coagulation.

3. Casein is very stable to high temperatures. They can withstand heating up to 140 °C for 20 minutes but whey protein is relatively heat labile. They are completely denatured by heating at 90 °C for 10 min.

4. Casein is a phosphoprotein containing an average of 0.85% phosphorous while whey protein does not contain any phosphorous. The phosphate of casein is an important contributor to remarkably high heat stability and calcium- induced coagulation of rennet- altered casein.

5. Casein contains very low sulfur (0.8%) whereas whey protein is relatively rich in sulfur (1%). The principle casein contains only methionine but whey protein contains a significant amount of cystine, cysteine, and methionine. These sulfur- containing amino acids are responsible for many changes in milk on heating.

6. Casein is synthesized only in the mammary gland and no other sources in nature. Some of the whey proteins β lactoglobulin, and α- lactalbumin are synthesized in the mammary gland but bovine serum albumin and immunoglobulin are not synthesized in the mammary gland but derived from blood.

7. The whey protein is dispersed in solution and has a simple quaternary structure. Casein has a complex quaternary structure and exists in milk as large colloidal aggregates.

3.1. General Introduction

Value addition refers to the conversion of a raw commodity or commodities into a processed product through the use of raw materials, labour, time and technology, all mixed in a way that ensures increased economic return. Even if the amount of processing in a product is much less, for example, any canned fruit can be considered as a processed product. Value added processing of horticultural food crops, mainly involves actions which changes fresh fruits and vegetables to create a greater value-added product for human consumption, which mostly includes canned products, juices, soups, sauces and dehydrated fruits and vegetables (Sharan, 2018). It results in diversification of agricultural activities, improves value addition opportunities and creates surplus for export of processed agro products. Processing often goes with perishable products like meat, milk, poultry, fruits and vegetables, often termed as High Value Products (HVPs), are in greater demand of processing (Sanal & Krishna, 2017). The demands for processed products are particularly strong in developing countries like China and India; mainly due to increasing industrialization and rapid urbanization, followed by elevated income levels, greater diversity in diets, and affinity towards premium-branded products (Sharan, 2018).

A. Construction, Use and Care of Light Microscope
Introduction
Microorganism are invisible to naked eyes, hence their detailed study requires a microscope. A microscope can be defined as an optical instrument consisting of a lens or a combination of lenses for getting enlarged or magnified images of minute objects. A simple microscope is essentially an ordinary magnifying lens held on an adjustable stand. Usually it gives magnification up to 20 times the size of the object. A compound microscope consists of two separate lens system – the objective and the eye piece. It gives much greater magnification (100-1500 times) than a simple microscope and is, therefore useful for viewing and examining very minute objects like protozoa, bacteria, yeast, mold and algae which cannot be seen with naked eyes.
The objective of this exercise is to familiarize the students with construction and the use of compound light microscope.
1. Construction of Microscope
A microscope contrite various parts in which principle parts are described below:
i) Frame work: All microscopes have a basic frame structure which includes the arm and base. All other parts are attached to this framework and the base support the microscope.
ii) Stage: It is a horizontal platform that supports the microscopic slide. It has a mechanical stage, which is used for holding and moving the slide around on the stage.

Introduction and Relevance
UHT processing of milk was developed to produce a shelf-stable product with minimal chemical changes as encountered with sterilized milk. Aseptically packaged UHT milk can have a shelf life of 06 to 12 months and does not need to be stored in a refrigerator. Its role in the market varies by region. In countries like the India, USA and Canada, where UHT milk occupies a smaller segment of the liquid milk market, it is often used as a backup option, reserved for situations when pasteurized milk is unavailable. Conversely, in regions such as Europe, (particularly France, Belgium, Spain and Germany), Asia, (China, Japan, Thailand and Vietnam) Latin America and (Brazil) in UHT milk is the dominant form of milk available and is consumed regularly. In markets where it is a backup option, UHT milk requires long-term stability of 6–12 months, while in regions where it is used daily, a shorter shelf life of three months or less is often sufficient.
Ultra-High Temperature (UHT) treatment involves heating milk to very high temperatures, typically between 130°C and 150°C, for a brief period of few seconds. This process results in commercially sterile milk, meaning that while not all bacteria are destroyed, those capable of growing under ambient storage conditions are effectively eliminated. An essential component of UHT processing is aseptic packaging, where the sterilized milk is filled into presterilized containers in a sterile environment. This combination ensures that the milk remains free from contamination and maintains its extended shelf life without requiring refrigeration.
Standards for UHT processing vary globally. In the European Union, a minimum temperature of 135°C with an appropriate holding time is mandated. In the United States, the standard for ultra-pasteurization is 280°F (137.8°C) for 2 seconds. Many international standards recommend 135°C for at least 1 second, though some countries do not specify minimum conditions. However, this level of treatment is often regarded as insufficient for completely destroying the spores of spoilage organisms, highlighting the importance of precise temperature-time combinations and the critical role of aseptic packaging in ensuring product safety and stability.

Milk: Composition, Nutritional Role, & Importance for Human Health Introduction
Milk is one of nature’s most complete and balanced foods, offering a rich source of essential nutrients such as proteins, fats, carbohydrates, vitamins, and minerals in forms that are easily digested and assimilated by the body. It serves as a cornerstone of human nutrition, providing critical support for growth, bone strength, and overall health. Known as the nectar of life, milk not only sustains life but also enhances immunity, forming the first bond between mother and infant.
An adequate intake of calcium, particularly during childhood, is critical for bone development and preventing osteoporosis in later life. Rich in calcium, phosphorus, and vitamin D, milk is vital for maintaining strong bones and teeth while preventing osteoporosis. Its high-quality proteins support muscle growthand recovery, while lactose and fats provide both immediate and sustained energy. Micronutrients like zinc, selenium, and whey proteins strengthen the immune system, and its high water content makes milk an excellent source of hydration. These qualities make it a protective food for all age groups, from infants to the elderly.
While milk naturally lacks iron and has low levels of vitamin D, fortification with additional nutrients enhances its nutritional profile, making it even more beneficial. Fortified milk helps address deficiencies, supports bone health, and boosts immunity, making it an ideal dietary option for diverse populations. For infants and children, milk is a near-perfect food, and for adults, it serves as an excellent dietary supplement, particularly for its calcium, protein, and vitamin content. Beyond its nutritional benefits, milk plays a significant role in culinary traditions and cultural practices across the globe.

Incentives are a powerful stimulus to transfer of technology. They can encourage researchers to get their results to market, a new career path for many of them. They can create a culture that attracts and retains entrepreneurial staff, scientists, students and technology transfer professionals (TTPs) that view the university as a career path and a vehicle for making a contribution to society. Working in a pro-entrepreneurship team of colleagues can also lead to getting people to think in terms of making a connection between their work and its application. Incentives can be short-term or long-term in nature, and can be tailored to individual or collective requirements. As it is likely to be challenging for universities to design incentives that cater to every researcher’s or TTP’s desire, they can design incentive programs to cater to various aspects of human. Nature.
12.1 Why incentives matter in this ecosystem
Incentives can be either financial and non-financial. These are needed to ensure that researchers and TTPs remain motivated, aligned with institutional goals, and committed to the long-term process of technology transfer and commercialization. Incentive programs serve several important purposes:
• Motivation: Incentives energize individuals to pursue innovation and commercialization, often over long- and uncertain-time horizons.
• Retention: By offering recognition, rewards, and career advancement opportunities, institutions can retain high-performing researchers and skilled TTPs.
• Alignment: Well-designed incentives ensure that the personal goals of stakeholders align with institutional and societal objectives.

31.1 Introduction
A fundamental element of quality assurance is good manufacturing practice (GMP), which ensures that goods are consistently produced and regulated according to the quality standards suitable for their intended application and as outlined by the marketing license. The primary objective of GMP is to mitigate risks in pharmaceutical manufacture, which may be categorised into two main types: erroneous labelling and cross-contamination/mix-ups. Primarily, manufacturers must guarantee that their goods are safe, effective, and of adequate quality; hence, risk assessment has become a crucial component of WHO quality assurance guidelines.
31.2 Implementation of HACCP
31.2.1 HACCP Preamble and Definitions Preamble
1. The introductory portion of this publication delineates the concepts of the Hazard Analysis and Critical Control Point (HACCP) system as endorsed by the Codex Alimentarius Commission. This section provides broad recommendations for system application, recognising that implementation specifics may differ according to the food operation’s unique conditions.
2. The HACCP system, grounded in scientific principles and methodical methodology, identifies specific dangers and implements control measures to assure food safety. It functions as an instrument for hazard evaluation and the implementation of control systems aimed at prevention rather than predominantly on final product testing. The HACCP system is flexible, allowing for modifications in equipment design, processing methods, or technology innovations.
3. HACCP is relevant across the whole food chain, from initial production to ultimate consumption, and its use must be guided by scientific knowledge on dangers to human health. In addition to improving food safety, the use of HACCP can provide other substantial advantages. Furthermore, the use of HACCP systems can streamline regulatory inspections and enhance international trade by increasing confidence in food safety.

B cell : It is one of the lymphocytes that have antigen-binding antibody molecules on the surface, that comprise the antibody-secreting plasma cells when mature, and that in mammals differentiate in the bone marrow. It is also called B lymphocyte.

B.T.U. : It stands for British thermal unit. The heat required to raise the temperature of one pond of the water through 1oF is called BTU.

Baby back ribs : Meaty pork ribs cut from the lower back rib section.

Introduction

COVID-19, a worldwide pandemic, addresses a deflationary stun for the worldwide economy. The outbreak of coronavirus has had a significant impact on public health worldwide and all stages of various industries’ supply chain and value chain. The 2019 novel coronavirus (2019-nCoV) or the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is rapidly spreading from its origin in Wuhan City of Hubei Province of China to the rest of the world. The infection began in bats and was communicated to people by inhalation or contact with infected droplets, and its infection time frame goes from 2 to 14 days. Primary manifestations in hospitalized patients with COVID-19 include fever (98%), cough/hack (76%), and myalgia or weakness (44%). Severe complications include acute respiratory distress syndrome (ARDS), RNAemia, acute cardiac injury, and multiple organ failure. However, exaggerated immune responses may lead to Cytokine Storm. Cytokine storm syndrome includes ARDS, shock, and multi-organ failure (Zheng et al., 2020). Many people are asymptomatic also, and the severity of this disease varies from person to person. The fatality rate is estimated to range from 2 to 3%. Typical/low white cell checks can analyze it with raised C-responsive protein (CRP), periodic modernized tomographic chest examination. The infection spreads quicker than its two predecessors, the SARS-CoV and Middle East respiratory disorder Covid (MERS-CoV); however, it has lower casualty. The worldwide effect of this new scourge is yet questionable (Singhal, T. (2020).

B cell: It is one of the lymphocytes that have antigen-binding antibody molecules on the surface, that comprise the antibody-secreting plasma cells when mature, and that in mammals differentiate in the bone marrow. It is also called B lymphocyte.

B.T.U.: It stands for British thermal unit. The heat required to raise the temperature of one pond of the water through 1oF is called BTU.

Baby back ribs: Meaty pork ribs cut from the lower back rib section.

Bacillary dysentery: It is a food borne illness characterized by very diarrhea and electrolyte loss alongwith intense abdominal pain, high fever and even death.

Bacillus spp: The genus Bacillus is generally aerobic, spore forming. Bacillus cereus also produces 2 types of toxins; a very fast aching emitting toxin which cause vomiting and a diarrhoeal toxin. Bacillus is a genus (Bacillus) of straight rod-shaped gram-positive usually aerobic bacteria producing endospores and including many saprophytes and some parasites produces disease (B. anthracis of anthrax).

Bacitracin: A polypeptide antibiotic isolated from a bacillus (Bacillus subtilis or B. licheniformis) and usually used against gram-positive bacteria.

Back Bacon / Canadianbacon: Bacon cut from the loin that has little fat and is cut into round or oblong slices.

Backward feed evaporator: In this evaporator, fresh feed enters the last and the coldest effect and continues on until the concentrated product leaves the first effect (Plate 54).

Bacon: Bacon is the meat cut from belly portion of hog carcass (a domestic swine esp. when weighing more than 120 pounds or 54 kilograms) and has high percentage of fat. It is cured and smoked.

INTRODUCTION

A food may have three attributes (1) Provide energy and nutrition (2) Give pleasure, i.e., enjoyable aroma, colour, and taste and (3) Health benefits. Functional food has physiological benefits and reduces the risk of chronic disease beyond basic nutrition.

Functional foods, also reported as ‘nutraceuticals’, ‘medical foods’ or ‘designer foods’ in the literature, were first described by Roberfroid (1999) as ‘Food similar in appearance to conventional food that is intended to be consumed as part of a normal diet, but has been modified to subserve physiological roles beyond the provision of simple nutrient requirements’. Examples are iodized salts, vitamin A and vitamin D fortified milk and yoghurt, folic acid enriched bread, soy products, wheat bran and oats products.

Introduction
 
Dairy ingredients are of significant importance in the formulation of many food products. Additionally, the incorporation of dairy ingredients, comprehensively recognized by the consumer as “natural” intensifies the odds of success of packaged foods and adds functional properties of the dairy ingredients in the targeted product. Dairy ingredients can be extracted from fluid milk in the form of cream, butter, condensed milk, dry milk, cheese, and whey products [1]. They provide beneficial functionality to foods, such as delivery of key nutrients, water management, fat-holding capacity, emulsification capability, viscosity creation, gel formation, and foam generation apart from providing desirable attributes of texture and flavour. Various ingredients and claims associated with dairy foods are shown in Table 3.1.

Introduction
 
Whilst food has long been used to improve health, our knowledge of the relationship between food components and health is now being used to improve food. Although most foods can be considered “functional”, in the context of this review, the term is reserved for foods and food components that have been demonstrated to provide specific health benefits beyond basic nutrition. Widespread interest in select foods that might promote health has resulted in the use of the term “functional foods”. Seaweeds are known for their richness in polysaccharides, minerals and certain vitamins [1], but they also contain bioactive substances like polysaccharides, proteins, lipids and polyphenols, with antibacterial, antiviral and antifungal, as well as many such other properties [2]. This gives seaweed great potential as a supplement in functional food or for the extraction of compounds. Physiologically active substances in marine algae are classified into two types based on the difference in the mechanisms viz. (i) non-absorbed high-molecular materials like dietary fibres, and (ii) low molecular materials, which are absorbed and which affect the maintenance of human homeostasis directly. Seaweeds are among the first marine organisms chemically analyzed, with more than 3,600 articles published describing 3,300 secondary metabolites from marine plants and algae, and they still remain an almost endless source of new bioactive compounds [3].

Introduction

Spices and aromatic herbs have been used since antiquity as preservatives, colorants, and flavour enhancers. Spices, which have long been the basis of traditional medicine in many countries, have also been the subject of study, particularly by the chemical, pharmaceutical, and food industries, because of their potential use for improving health. Both in-vitro and in-vivo studies have demonstrated how these substances act as antioxidants, digestive stimulants, and hypolipidemics and show antibacterial, anti-inflammatory, antiviral, and anti-carcenogenic activities. These beneficial physiological effects may also have possible preventative applications in a variety of pathologies. The chapter presents an overview of the potential of spices and aromatic herbs as functional foods ingredients.

Introduction

Encapsulation may be defined as a process to entrap one substance (active agent) within another substance (wall material). The encapsulated substance, except active agent, can be called the core, fill, active, internal or payload phase. The substance that is encapsulating is often called the coating, membrane, shell, capsule, carrier material, external phase, or matrix. In the food industry, encapsulation process can be applied for a variety of reasons. Encapsulation is a useful tool to improve delivery of bioactive molecules (e.g. antioxidants, minerals, vitamins, phytosterols, lutein, fatty acids, lycopene) and living cells  (e.g. probiotics) into foods [1]. In most cases, encapsulation refers to a technology in which the bioactive components are completely enveloped, covered and protected by a physical barrier, without any protrusion of the bioactive components. Also, encapsulation has been defined as a technology of packaging solids, liquids, or gaseous materials in small capsules that release their contents at controlled rates over prolonged periods and under specific conditions. Produced particles usually have diameters of a few nm to a few mm.

Introduction
 
The future of functional foods is bright and there are several reasons for the growing interest in “functional” foods, such as awareness of personal health deterioration, led by busy lifestyles with poor choices of convenience foods and insufficient exercise; increased incidence of self-medication; increased level of information from health authorities and media on nutrition and the link between diet and health; scientific developments in nutrition research; and a crowded and competitive food market, characterized by pressurized margins. Now, the consumer is well aware of the relationship between diet and health. Consumers understand that the risk for chronic diseases such as heart disease, stroke, colon cancer, and type II diabetes is possibly preventable by modification in their diet pattern. This scientific progress in understanding the relationship of diet to disease, along with increasing health care costs and the consumer’s desire to initiate healthy eating and lifestyle habits, makes this a potentially fertile ground for the development of food products that meet these demands.