eChapters

To look into the nutritional security around the globe, FAO and WHO, have undertaken the analysis of policies. They have covered many issues which include; World Summit on Food Security, pillars of food security and availability, access, utilization, stability, effects of food insecurity, stunting and chronic nutritional deficiencies, depression, anxiety and sleep disorders. There are many challenges to achieve food security. These include global water crisis, land degradation, climate change, agricultural diseases, food versus fuel, politics, food sovereignty, food wastes, risks to food security, population growth, fossil fuel dependence, homogeneity in the global food supply, price setting, land use change, global catastrophic risks, agricultural subsidies and role towards children.

Abstract

Starch is a naturally occurring biodegradable polymer and readily available from agriculture. It is widely used in food and non-food applications and isolated from crops such as maize, potatoes, tapioca, rice and wheat. However, in recent years substantial progress has been made on the molecular structure of cereal and tuber starches and their impact on functionality, the following is a subjective summary that presents status of knowledge on isolation of starch from non-conventional source like Acorus calamus (sweet flag). The focus is on potential application of the source as food and non-food derivatives. Extensive work has been carried out on agronomic and phenotypic properties for most tropical crops, but relatively little study has been carried out on the properties which are of direct technical and economic interest to competitive food and non-food industry. Sweet flag (Acorus calamus L.) is an emergent macrophyte, originating in Asia, commonly found in European wetlands. Rhizome is the most important storage organ in Acorus. It contains considerable amounts of carbohydrates and nitrogen-rich compound. This species is growing to 6 feet, with long lanceolate leaves %-inch wide with pronounced midrib, aromatic, with stout rhizomes. The major proportion of total non-structural carbohydrates in rhizomes is starch (91-93% and 51-64% in rhizomes and roots, respectively). However, amount of starch varies among the organs as well. Rhizomes contain the highest amount of starch followed by branched roots and finally by unbranched roots. Rhizomes comprises single to compound grains, with clusters present in high frequencies. Non-conventional food resources and their value addition have attracted attention in the recent years for their potential use as functional ingredients in food formulations, besides food they have also been used for non-food applications. So there is tremendous potential for the profitable commercial use of these tropical starches and exploring them for further use.

Abstract

The production of natural food colours through bio-processing is preferred now-a-days due to health related issues associated with their synthetic counterparts. Microorganisms are generally preferred sources as these have high production rate, high growth rate, ease of handling under controlled conditions and absence of seasonal variations. Biopigments can be produced from different genera of bacteria, yeast and mold. Different types and colours of pigments have been produced from microorganisms depending upon their origin, media components and process conditions. The biopigments have variety of applications in food industry such as food additives, food preservatives, biological control agents and nutritional supplements. These have also been associated with therapeutic, and antioxidant properties. Lycopene from B. trispora is used as a food ingredient in dietary supplement. Astaxanthin from X. dendrortious has also been reported to have beneficial effect on human health as it possesses strong antioxidant properties. Arpink red P. oxalicum is used as red colorant in meat products, non-alcoholic drinks, milk products and confectionary. 0- carotene is considered as leading food colorant in world and is applied to range of baked, dairy and canned foods to improve their appearance. The antioxidant andprecursory properties of0-carotene increase its applications in pet foods. Zeaxanthin is used as additives in poultry fed to intensify the colour of the egg yolk. Monascus pigments are utilized for coloration of sausages, ham, marine products and tomato ketchup. Monascus extract is also marketed as dietetic supplement in Japan. Thus, the biopigments can serve as a chromophore for colorant production with broad spectrum of colours and wide applications.

Poultry meat-50 g for each method
Cook the poultry meat according to the methods given in the table and record the cooking effects

Practical case studies provide a direct connection between technical knowledge and the real-world challenges faced by ice cream manufacturers and managers. This chapter brings together applied situations involving formulation, overrun, texture, freezing, storage, cold-chain management, production losses, changeovers, product launches, market returns, profitability, and operational decision-making. Each case encourages readers to identify symptoms, determine probable causes, evaluate technical and commercial consequences, and develop corrective actions. The cases demonstrate that ice cream performance depends on the interaction of formulation, processing, quality, equipment, distribution, consumer expectations, and business strategy. The chapter is particularly valuable for students, technologists, entrepreneurs, managers, consultants, and industry professionals seeking practical problem-solving skills.

Experiment No.- 1
AIM
To determine the sucrose content in condensed or evaporated milk using the Lane-Eynon method.
Principle
The Lane-Eynon method is used to measure sucrose in condensed milk by utilizing the reducing properties of sugars. Sucrose, being a non-reducing sugar, is first hydrolyzed into glucose and fructose (reducing sugars). These reducing sugars then react with Fehling’s solution, producing a brick-red precipitate of cuprous oxide (Cu2O) when heated. This allows for the accurate determination of sucrose content after the hydrolysis (inversion) process.
Reactions
• Reaction of Fehling’s Solution
• CuSO4 + NaOH → Cu(OH)2 + Na2SO4
• Cu(OH)2 + Reducing Sugar (RCHO) → Cu2O (brick-red precipitate) + RCOOH
This reaction forms cuprous oxide (Cu2O), a brick-red precipitate. The intensity of this precipitate correlates with the concentration of reducing sugar in the sample, providing a way to determine sucrose content.

Introduction

Food is a resource of vital importance and its safety in the whole chain, from producer to consumer, is a worldwide concern. In fact, as food trade expands throughout the world, food safety has become a shared concern among all consumers. This issue began to be taken seriously at a period where there is a significant increase of illness caused by infected food in both developed and developing countries. Unsafe food contains hazardous agents or contaminants that can make people sick, immediately or by increasing their risk of chronic disease. Contaminants can enter food at many different points in the food production process, and can occur naturally or as the result of poor or inadequate production practices. International competitiveness depends more on quality and safety concerns than on prices. Governments and local authorities intervene in the market place in setting technical regulations; and sanitary and phytosanitary measures. This encouraged food processing companies around the world to implement food safety management systems which guarantees the production and distribution of safe foods. Prerequisite Programs (PRPs) are considered as the integral part of food safety standards and audit schemes all over the world. It is essential to implement food safety programmes in every company to check the chances of illness or injury due to unsafe food.

Abstract

The concern of consumers for convenience and healthy foods involves many parameters like functional and nutraceutical properties, ease to prepare, good quality, economics as well as good nutritional profile of the food. This trend of convenience has been increasing in today's world because of the societal changes like the aging of population, the increasing entry of women into the labor force, the change to a multi-cultured society and the increasing polarization between poor and rich. Value addition to the traditional sources is a medium to bring variety products into the market. Cereal starches possess a wide application in the food industry as an additive but the native starch is not widely used in food industry due to its poor functional properties. Pregelatinized starches and flours have become important in processed foods because the functional properties of the starches and flours are improved over the native starches and cereal flours. Modification is a process which alters the structure of the starch in the wheat endosperm by affecting the hydrogen bonds in a controllable manner. Modification of starch and flours is generally done by several methods such as chemical degradation, physical alteration or enzymatic transformation. The primary objective of modification is to reduce the hot viscosity of the starch paste so that higher concentrations of starch can be dispersed without excessive thickening. The pregelatinized flours offer a tremendous number of functional benefits to variety of foods such as bakeries, snacks, beverages as well as nutritional foods. The various functional benefits of pre-gelatinized starch/flour as an additive are classified as fat replacer, texture improver, stabilizer, emulsifier, thickening agent and for freeze-thaw stability. Keywords: Pregelatinization, modification, functionality, cereal flour.

Abstract

Prebiotics are the non-digestible food ingredients that beneficially affect the host by selectively stimulating the growth and/or activity of one or limited number of bacteria in the colon. These are heat resistant components which remain intact during the baking process; this allows prebiotics to be incorporated into every day food choices. Consumption of non-digestible ingredients allows the growth ofbio cultures by reaching the intestine unaffected by the digestion process resulting in improvement of digestive health. Prebiotics reach the intestine in an unaltered form and shows the prebiotic effect when there is an increase in the activity of healthy bacteria in the human intestine. The prebiotics stimulate the growth of healthy bacteria such as bifidobacteria and lactobacilli in the gut and increase resistance to invading pathogens. Functional foods increase consumer choice by adding prebiotics to every day food items such as cereals, biscuits, breads, table spreads, drinks, and yoghurts. By continuing to eat and drink common foods, but choosing functional alternatives (such as bread containing prebiotics) dietary requirements can be met, without significant changes to foodpreferences. Some common example of cereal based products containing prebiotics are oat-based prebiotic drink, Breakstone's and Knudsen live active cottage cheese, prebiotic yogurt- Attune and Muller vitality, multi-fruit drink enrich and flavoured water enrich, prebiotic flavoured water, prebiotic capsules, dried fruit bars, prebiotic powder- Biocare fructo-oligosaccharides, Newtree purplaisir chocolate spread. The claims are constipation relief, suppression of diarrhea and reduction of the risks of osteoporosis, atherosclerotic cardiovascular disease associated with dyslipidemia and insulin resistance, obesity and possibly Type-2 diabetes. At present, claims about reduction of disease risk are only tentative and further research is needed.

Introduction

Probiotics, prebiotics and synbiotics are interrelated terms. Probiotic is discussed in detail in previous chapter. In this chapter, prebiotics and its effect on gut along with probiotics are emphasized. Prebiotics can be defined as non digestible food ingredients that stimulate the growth and/or activity of bacteria in the digestive system in ways claimed to be beneficial to health. They were f irst identified and named by Marcel Roberfroid in 1995. He defined prebiotic as a selectively fermented ingredient that allows specific changes, both in the composition and/or activity in the gastrointestinal microflora that confers benefits upon host well-being and health. Further, FAO defined prebiotic as “a non-viable food component that confers a health benefit on the host associated with modulation of the microbiota.”Whereas the term synbiotics refers to a product that contains both probiotics and prebiotics in the form of synergism. A synbiotic has been defined as “a mixture of prebiotics and probiotics that beneficially affect the host by improving the survival and implantation of live microbial dietary supplements in the gastrointestinal tract, by selectively stimulating the growth and activating the metabolism of one or a limited number of health promoting bacteria, and thus improving host welfare”. As described above, the combined use of prebiotics and probiotics is often described as synbiotic, but the United Nations Food & Agriculture Organization (FAO) recommends that the term “synbiotic” be used only if the net health benefit is synergistic.

According to Fereshteh et al. (2020) microorganisms use biofilm to protect themselves against the harmful effects of the host body immune system and the surrounding environment. This helps them to survive against the anti-microbial agents. Inhibition of bacterial biofilm formation is very time consuming and expensive. Newer computational tools have been found to be a good option to predict the inhibition of biofilm peptides. In a computational prediction tool a large number of peptide sequences can be screened to select the peptides for validation. Vectors are extracted from the peptide primary structure and classification algorithms are carried out which includes SVM, random forest and k-nearest neighbour to evaluate their performance. Using a combination of features, helps in good evaluation. National Institute of Health (NIH) has found out that 80% of bacterial pathogens form biofilms and their multicellular structure makes them resistant to the hosts’ defense systems and traditional antimicrobials than planktonic bacteria. Recently, the use of Antimicrobial peptides

Introduction

For agricultural applications as well as for research work, often we need to prepare solutions of different agrochemicals of varying strength, as per the requirements. Agrochemicals are used to help crop plants grow, produce, and protect them from biotic/abiotic stresses. Many of the agrochemicals (insecticides, herbicides, fungicides, rodenticides, molluscicides, nematicides, etc.) are used to protect the crop from insect pests and diseases. However, some of them (fertilizers and soil conditioners) are used to augment crop yield. While in the previous chapter (Chapter 8) we discussed about preparation of laboratory solutions, in this chapter we will briefly discuss about the preparation of different agrochemicals.

Agrochemical/agrichemical is a generalized term used for the chemicals applied in agriculture. In general, it also includes the feed-supplements used in f isheries and animal husbandry.

Agrochemicals

Fertilizers

Fertilizers are used to augment growth of crop plants and to mitigate nutrient deficiency in the soil. Fertilisers can be organic (containing natural substances, prepared through natural processes) and inorganic fertilisers (synthetic fertilizers, manufactured artificially using chemical processes using natural deposits, by chemical alterations).

Soil Conditioners

Soil conditioners include manure, livestock and leaf compost, peat that help making the soil in good condition. They are laid on the top soil and then mixed properly. They not only increase nutrient contents in the soil but also improve aeration and water holding capacity.

Introduction

Introduction to Feasibility Reports

A feasibility report is a detailed analysis of the viability, sustainability, and profitability of a proposed project. It is crucial for decision-making and project planning, particularly in the dairy industry where multiple variables affect the outcomes.

Objectives of Feasibility Reports • Assess economic viability.

• Analyse technical requirements.

• Evaluate operational sustainability.

• Provide a roadmap for project implementation.

• Decision-Making Tool: Helps stakeholders decide whether to proceed with a project. Hence, minimize risks through identification of potential obstacles and informed decision-making.

• Resource Optimization: Ensures efficient use of resources like time, money, and labour.

• Stakeholder Communication: Provides a structured overview of the project’s potential.

Introduction

Different types of culture media are used for culturing microbes and plant tissues. Preparing as well as sterilizing them properly following appropriate method with safety for the desired purpose are important parts of research work. In this chapter, we will discuss briefly about different types of media, their composition, preparation and sterilization in laboratory for microbial or plant tissue culture.

Culture Medium

Ingredients mixed to prepare solid, liquid or semi-solid substance to support the growth of microorganism or plant tissue in vitro (outside the body) is called culture medium. Frequently, it becomes important to grow microorganism or plant/animal tissue in vitro for any or all of the following purposes:

• To study characteristic features or properties of microorganisms.

• To identify the causal organism(s) of infection in a clinical sample, so that appropriate remedy can be determined.

• To culture/multiply microorganism or plant/animal tissues in vitro for research or commercial purposes.

• To prepare/produce biological molecules, vaccines, antigens, proteins, metabolites, etc. in vitro for research or industrial purposes.

Ingredients; milk fat (cream)-12%, non-fat milk solids (skim milk powder)-11%, sugar-15%, stabilizer-0.2%, emulsifier-0.2%, flavors-a pinch Basic ice cream preparation: 1. Mix the ingredients 2. Pasteurization 3. Homogenization 4. Ageing (allowing to standby 24 hours at 4.440C) 5. Freezing (the mix is frozen at 5.5560c) 6. The freezing must be quick in order to prevent formation of large crystals. The ice cream is semi solid at this temperature. 7. The ice cream is finally frozen to a hard mass at about -30C.

Abstract

Pomegranates are usually consumed as fresh fruit, with only a limited quantity being processed into products like juice, syrup, beverages, wine, confectionary and anardana. The special structure of fruit is one of main factors which limit its industrial processing. Knowing the importance of health benefits of pomegranate and all related aspects, the present study was carried out in order to utilize pomegranate fruit for preparation of pomegranate mouth freshener. Arils of two cultivars like Ganesh and Araktaweredried by two drying methods viz., sun drying (SD) and cabinet drying (CD).Nutritive and palatable mouth freshener was prepared from anardana of two different cultivars as Ganesh (G) and Arakta (A). Production cost of 100g pomegranate mouth freshener is about Rs.55/. It was noticed that moisture content, acidity and ascorbic acid content decreased at the end of storage.

The RTS beverage was prepared by using freshly prepared carrot juice, sour orange juice, water and sugar as per FPO specification.

Introduction
Ocular micrometer and stage micrometer are used to measure the size of microorganisms. The Ocular micrometer consists of a circular disk of glass which has graduations engraved on surface. A stage micrometer or objective micrometer has scribed lines on it that are exactly 0.01mm (10 micrometer) apart. Ocular micrometer is calibrated for the objective first. To calibrate the ocular micrometer for a given objective, the two scales are superimposed and determined how many of the ocular gradation coincide with one graduation on the stage micrometer scales. Thus, the distance between the graduations on the ocular micrometer can be determined and so the size of the organism can be determined.
Materials
1. Ocular micrometer
2. Stage micrometer
3. Microscope
4. Stained slides of yeast or bacteria

1. Preparation of Standard Sodium Hydroxide Solution

 Introduction

 When oxalic acid reacts sodium hydroxide, sodium oxalate and water are

form. Phenolphthalein indicator detects the end point by its characteristics

colour change.

 Apparatus

 Burettes, pipette, volumetric flask, beaker

 Reagents

 • Oxalic acid crystals

 • Sodium hydroxide – 0.1 N approximate

 • Phenolphthalein solution – 0.1 percent in ethyl alcohol

Procedure Preparation of 0.1 N oxalic acid solution 1. Weight accurately 1.575 g of oxalic acid into a 250 ml beaker using a weighing bottle. 2. Add some distilled water to the beaker and dissolve the oxalic acid with the help of glass rod. 3. Transfer the solution into a 250 ml volumetric flask through a funnel. 4. Wash the beaker 3- 4 times with small quantities of distilled water and transfer the washing into the flask. 5. Make up the volume up to the mark with distilled water and mix thoroughly. 6. Preparation of approximately 0.1 N sodium hydroxide solution 7. Weigh about 4.25 g of sodium hydroxide pellets and dissolve into 200 ml of distilled water.

Bacterial species vary greatly in the ability of their cultures to remain alive after the completion of growth. Some species such as Neisseria gonorrhoeae and Streptobacillus are poorly viable and their cultures usually die out within a few day, whether kept at 37°C at room temperature or at 4-5°C; thus, they must be sub cultivated every 2-4 days for maintenance in the laboratory. Other species are much hardier,. especially sporing species which may remain viable for many years. There are also many non - sporing species (enterobacteriaceae) whose cultures, under suitable conditions, commonly remain viable for many months and often for as much as 10-20 years.

Preservation of raw milk is essential to maintain its quality during storage and transportation before processing. One of the primary methods for extending its storage life is refrigeration, where milk is cooled to 2°C to 4°C immediately after milking. This significantly slows down the microbial growth and enzymatic activity. Bulk milk coolers (BMCs) are vital in achieving rapid cooling, especially in collection centres or dairy farms, where they ensure that milk remains fresh during storage and transportation. BMCs are particularly essential for regions with inadequate cold chain infrastructure, as they help maintain milk quality until it reaches processing facilities.
In addition to refrigeration, thermisation is another method used to preserve raw milk. Thermisation, a mild heat treatment, is widely used to extend the shelf life of raw milk, particularly when it needs to be transported over long distances. This process involves heating the milk to 57–68°C for 15 seconds, effectively reducing the microbial load while preserving the milk’s natural characteristics. Thermisation is typically followed by refrigerated storage, where milk is cooled to temperatures between 2° and 4°C to inhibit further microbial growth. The combination of thermisation and refrigeration ensures that milk retains its freshness and safety until it reaches processing facilities, even in regions with extended supply chains.
Raw milk also benefits from its natural inhibitors, such as lactoperoxidase, lactoferrin, and immunoglobulins, which exhibit antimicrobial properties and slow down bacterial growth. The lactoperoxidase system, for instance, can be activated by adding small amounts of hydrogen peroxide and thiocyanate to enhance milk’s preservation in the absence of refrigeration. The use of chemicals like hydrogen peroxide (H2O2) for milk preservation is not permitted in India due to safety and quality concerns. However, the FAO recommends its application under strict regulatory conditions in some countries as part of the lactoperoxidase system, particularly in regions where refrigeration is unavailable.

Introduction

Food is the most vital prerequisite for sustenance of human life and provides adequate nutrients for the metabolism. Recently several hypothesis supports that, beyond meeting nutritional needs, they play beneficial roles in improving health, and minimize the risk of illnesses (Roberfroid, 2000a). In this way, a novel term “functional food” was introduced and plays an outstanding role in improving the overall conditions of the human body (e.g. pre- and probiotics), lessening the threat of some ailments (e.g. cholesterol-lowering products), and also as therapeutics for several diseases (Roberfroid, 2000a, Roberfroid, 2000b, Mollet and Rowland, 2002).

Recently throughout the world, an extensive array of functional foods were developed which includes prebiotics, probiotics, synbiotics, foods supplemented with anthocyanins, phytosterols, isoflavones, antioxidants, and fat, sugar and salt-reduced foods. Amongst them, probiotic functional food has exerted beneficial effects on the health and is comprised of both probiotic dairy foods and probiotic non-dairy foods. The demand for dairy probiotic foods are increasing as it attributes to the beneficial health effects on the host by improving the survival of gastrointestinal microflora, production of bioactive compounds and prevention of lactose intolerance. Consequently, production of these foods is a significant research importance for both food manufacturing units and research institutes.

Probiotics are the important ingredients for foods with a “healthy” image. It could be combined with other beneficial ingredients or simply used to supplement the natural functional qualities of whole foods. The amalgamation of probiotic bacteria with nutrient rich foods, like dairy products, will have the additional value of improving consumer nutrition.

There are potential beneficial medicinal uses of probiotics for which research is limited and only preliminary results are available. Recent research on the molecular biology and genomics of Lactobacillus has focused on the interaction with the immune system, anti-cancer potential, and potential as a biotherapeutic agent in case of antibiotic-associated diarrhoea, traveller‘s diarrhoea, pediatric diarrhoea, inflammatory bowel diseases and irritable bowel syndrome (Ljungh et al., 2009). Studies of some probiotics tested in vitro on human epithelial cell lines have shown that they could block the adhesion of shiga toxin producing E. coli (Sherman et al., 2005). These are claimed to be health promoting and/ or disease preventing beyond the basic function of supplying nutrients. Probiotic foods are emerging as an important category of food supplements. Earlier workers have shown the inhibition of diarrhea caused by E.coli using probiotic lactic acid bacteria and yeast (Yan and Polk, 2006). Ratledge et al. (2002) have studied the biochemistry and molecular biology of lipid accumulation in oleaginous microorganisms.

Abstract

Nutraceuticals have potential to be used as food supplement, preventive medicine and the growing evidence points in the direction that certain foods fight and or prevent against diseases. The development of next generation nutraceutical “super foods” or products consist of value-addition in the traditional natural diets.The word Nutraceuticals combine ‘nutrition’ and ‘pharmaceuticals’ to mean that they can be used as preventive drugs or food supplements. The increase in knowledge about the immune response unveils the fact that besides immunological disorders and infections the outcome of many other diseases like diabetes, hypercholesterolemia, rheumatoid arthritis, etc. can be modified by modulating Immune response. Hence, the efficient immunomodulators can be employed to prevent or cure the diseases as immunotherapeutic agents. The most acceptable alternative therapeutic agents are the edible sources. Hence the researchers shifted towards formation of nutraceuticals or foods acting as medicine, a concept which was given by Hippocrates long back.

Introduction

The term probiotic is derived from Greek and means ‘for life’ as opposed to antibiotics which means ‘against life’. The history of probiotics began with the consumption of fermented foods which was first observed in ancient Greeks and Romans [1]. The term, probiotic, as is used today was first used by Parker in 1974. Parker defined ‘probiotic’ as substances and organisms which contribute to intestinal microbial balance. In 1989, the term was modified further by Fuller. Thus, probiotic is a live microbial supplement which affects the host’s health positively by improving its intestinal microbial balance. This definition was broadened to include mono or mixed culture of live microorganisms which benefits animals or man by improving the properties of the indigenous microflora [2]. Some authors have interpreted probiotics as microbial cell preparations or components of microbial cells that have a beneficial effect on the health and well-being of the host.

Introduction

Let food be thy medicine and medicine be thy food- the age-old quote by Hippocrates, is certainly the tenet of today. Microbial cultures have been used for millenniums in food and alcoholic fermentations and in the past century have undergone scientific scrutiny for their ability to prevent and cure a variety of diseases. Later it became popular in improving human and farm animal nutritive values and recently in aquaculture also. The growing interest in the understanding the role of food in human health has moved from its primary role as a source of energy to the subtle action of biologically active food components on human health. Henceforth functional foods or nutraceuticals are of great demand in present time. The growing scientific evidence suggests that the food supplements containing beneficial bacteria can provide an array of benefits to the host. Now a day’s probiotic fermented foods are in great demand due to the awareness of beneficial aspects of probiotics among people. Several industries focused on the improvement of conventional fermented foods scientifically in order to fetch good market for their products. Particularly, animal feed companies and researchers have been looking for alternative products and strategies that can help to maintain animal gut health in order to prevent or reduce the prevalence of pathogens in the food chain. After understanding the mechanism and beneficial aspects of probiotics in detail at molecular level in earlier chapters, now it is relevant to discuss the applied part of probiotics. In this chapter, commercial importance of probiotics and probiotics derived products at industrial level are emphasized.

Introduction

During the first 50 years of the 20th century, scientific focus was on the identification of essential elements and their role in the prevention of various dietary deficiency diseases. This emphasis on nutrient deficiencies or “undernutrition” shifted dramatically, however, during the 1970s when diseases linked to excess and “overnutrition” became a major public health concern. “Let food be thy medicine and medicine be thy food”, the age old quote by Hippocrates is the ideology of today’s health conscious population. Eli Metchnikoff, the Russian Nobel prize winner was the first one to recognize the beneficial role of selected bacteria on gastrointestinal tract of humans. Subsequently the “Theory of Longevity” by Metchnikoff was correlated with prolonged youth and a healthy old age, observed largely in Balkan peasants of those times, who used cultured milks in their diet (Kaufmann, 2008). Since then, the quest continues for understanding the role of wide range of food components and nutrients in enhancing health or preventing chronic diseases. The research in this field has resulted in a plethora of new labels for pro-, pre- and synbiotics.

Abstract

The gut microbiota, microbe population living in the human large intestine is generally associated with various metabolic functions and it influences health and well-being. Taking into account the major role gut microbiota play in the normal functioning of the body and the different functions it accomplishes, experts nowadays consider it as an “organ”. However, it is an “acquired” organ, as babies are born sterile; that is, intestine colonisation starts right after birth and evolves as we grow. Each of us has a unique microbiota, as the fingerprints, it always fullfils the same physiological functions and they have a direct impact on our health. Gut microbiota consists of a complex of microorganism species that live in the digestive tracts of animals and is the largest reservoir of microorganisms mutual to humans. Gut microorganisms benefit the host by gleaning the energy from thefermentation of undigested carbohydrates and the subsequent absorption of short-chain fatty acids. The most important of these fatty acids are butyrates, metabolised by the colonic epithelium; propionates by the liver; and acetates by the muscle tissue. The human body carries about 100 trillion microorganisms in its intestines, a number ten times greater than the total number of human cells in the body Guarner and Malagelada, 2003). Gut microbiota, particularly the probiotics, have beneficial effects on gastroenteritis resistance, blood lipids, antitumor properties, lactose tolerance and gastrointestinal immunity. Probiotics have role in reducing the risk of rotavirus-induced diarrhea and colon cancer. The survivability of any probiotic bacteria till a longer time in the gut can be enhanced by the supplementation of prebiotics. Prebiotics are non-digestible food ingredients that benefit the host by selectively stimulating the growth or activity of one or a limited number of bacteria in the colon. There are so many prebiotics which stimulate the growth and survivability of probiotic bacteria such as fructo-oligosaccharide (FOS), inulin, galacto-oligosaccharide (GOS), polydextrose (PDX), acacia gum (GUM), lactulose (LOS), lactitol etc. Many research studies have been done on above mentioned prebiotics. Mostly inulin-type fructans have been used as functional food ingredients. The prebiotics are known for reduction of disease risk such as constipation relief, suppression of diarrhea and reduction of the risks of osteoporosis, atherosclerotic cardiovascular disease associated with dyslipidemia and insulin resistance, obesity and possibly type-2 diabetes. Probiotics are beneficial microbes that confer a realistic health benefit on the host, which in combination with prebiotics, (indigestible dietary fibre/carbohydrate), also confer a health benefit on the host via products resulting from anaerobic fermentation. The combination of probiotics and prebiotics in a synbiotic might improve the survival of the bacteria crossing the upper part of the gastrointestinal tract, thereby enhancing their effects in the large bowel and immune-modulatory ability.

In 1907, the Russian Nobel laureate, Dr. Elie Metchnikoff, fascinated by the unusual longevity of Bulgarian farmers, consuming beneficial lactic acid bacteria by way of traditional fermented milk products, suggested that regular intake of friendly microbes could benefit human health. Although Dr. Metchnikoff ’s recommended bacteria for the purpose proved largely ineffective, the theory was carried forward with moderate success until the war on bacteria began because of the advent of sulfa drugs and antibiotics (Scott, 2012). However soon after their rampant use and the realization of the rapid spread of antimicrobial resistance, researchers turned yet again to ecological notions and the time was fertile for the advent of probiotics. By the 1980’s the term had stabilized both in medical and veterinary literature. The mid 1990’s witnessed a surge of exponential studies on probiotics, the definition of Probiotics emerged in 2001 and the years thereafter saw tremendous progress in the science of probiotics.

Almost simultaneous was the study on the study of the intestinal microbiota when Alfred Nissile demonstrated that transferring members of the human gut microbiota to healthy typhoid carriers helped in Salmonella being cleansed from their system (Nissle, 1916). It was then, that the importance of the gut microbes in prevention of infection became evident. What followed was the advent of culture independent, modern biotechnological tools for whole genome sequencing and the successful launch of two most ambitious projects - Human Microbiome project (Turnbaugh et al., 2007) and the Met Hit project (Metahit, 2009; Qin et al., 2010). They provided fascinating insights into the highly complex microbial ecosystem harboured by the human body. The term Microbiome (refers to the collective genome of our microbial inhabitants) had entered literature in 2001 and the concept of gut microbiota in maintaining overall health gained wider attention.

Introduction

Thermal processing is an important treatment for food preservation is, especially in the manufacture of shelf-stable foods with specific nutritional properties. It is also indispensable for determining the sensory properties, particularly, colour, flavour, and texture of fried, baked, and roasted products. As a result of heat treatment, chemical changes induced, and a plethora of new molecules are formed in foods, some of which have been claimed to be health-promoting antioxidants and anti-mutagens. However, reports suggest that thermal processing leads to the formation of certain toxic compounds, so-called process-induced contaminants in foods. The process-induced toxicants are certain compounds which are formed in food as a result of food processing, exert adverse toxicological effects, and cause potential health risks to humans. Acrylamide, furan, chloropropanols, heterocyclic aromatic amines, polycyclic aromatic hydrocarbons, and N-nitrosamines are some well-known examples of thermal process contaminants.

Abstract

Kulfi is a mixture of fat; sugar and milk solid not fat together with added flavour. So diabetics people on restricted diets cannot consume kulfi having normal ingredients. Now-a-days, consumers demand for low fat/calorie products has significantly raised in an attempt to limit health problems, to lose or stabilize their weight and to work within the frame of a healthier diet. The role of fat replacers and sugar substitutes in the successful manufacture of these products is crucial. Development of sugar free kulfi would certainly ensure diabetic/obese people to enjoy ice cream/ kulfi. The current study is to standardize the kulfi recipe with fat and calorie replacer. Basic formula mix consist of Whole milk 60 percent, SMP 12 percent, Sugar 13 percent, Cream 15 percent and Stabilizer 0.5 percent where the whole milk was replaced by skim milk, cream and sugar was replaced by maltodextrin as a bulking agent and aspartame respectively alongwith sodium alginate as a stabilizer. The replacers were incorporated at four different levels of 0, 25, 50, 75 and 100 percent to the basic formula mix. Freezing of above kulfi mix was done by traditional method and softy freezer method. Sensory evaluation was carried out by using 9-point hedonic scale through semi-trained judges and results were statistically analyzed. On the basis of sensory scores it was revealed that sugar could be replaced 100% by artificial sweetener, bulking agent (Maltodextrin). It was found from the sensory scores that the milk fat in kulfi can be replaced upto 75 percent by sodium caseinate thereafter increase in caseinate gives prominent flavour of casein and kulfi body becomes thick and hard. It was concluded from the results that 100 percent sugar replacement was found feasible in preparation of low fat and low calorie kulfi. Results of the low fat and low calorie kulfi mix indicated that there was 32.29 percent reduction in the calorific value of experimental kulfi as compared to the control sample (traditional). It was concluded from the results that the kulfi with 75 percent less fat and 100 percent sugar free can be prepared with low cost in comparison with control.

Introduction

India, with its unique agro climatic conditions is capable of producing a wide variety of fruits and vegetables. These highly perishable produce in both fresh and processed forms are of high demand in domestic as well as international market. Nowadays farming is becoming an enterprise and shifting towards consumer driven agriculture. Produce quality measurement is of utmost importance as consumers are more quality conscious and ready to pay higher for quality. Consumer preference is moving towards healthy food, minimally processed and, fresh cut fruits and vegetables, prepared salads, ready to cook, ready to eat and ready to drink health beverages. Hence health, nutrition, convenience, quality and safety of produce should be guaranteed to the consumers. Due to globalisation of production and trade, legislations and standards for markets are strict and product quality is the necessity for competitive markets.

Quality of a produce, based on subjective and objective measurement, is defined differently by consumers, wholesalers, retailers, processors or any other who is associated with it and varies with market either national or international. Quality of foods may be defined as the composite of those characteristics that differentiate individual units of a product, and have significance in determining the degree of acceptability of that unit to the user (Kramer, 1965). According to ISO (International Organisation of Standardisation), “Food quality is the totality of features and characteristics of a product that bear on its ability to satisfy stated or implied needs” and as per CAC (2003), “Food safety is the assurance that food will not cause harm to the consumer when it is prepared and/or consumed according to its intended use”. Food safety is the most important component of quality.

3.1. General Introduction

Value addition refers to the conversion of a raw commodity or commodities into a processed product through the use of raw materials, labour, time and technology, all mixed in a way that ensures increased economic return. Even if the amount of processing in a product is much less, for example, any canned fruit can be considered as a processed product. Value added processing of horticultural food crops, mainly involves actions which changes fresh fruits and vegetables to create a greater value-added product for human consumption, which mostly includes canned products, juices, soups, sauces and dehydrated fruits and vegetables (Sharan, 2018). It results in diversification of agricultural activities, improves value addition opportunities and creates surplus for export of processed agro products. Processing often goes with perishable products like meat, milk, poultry, fruits and vegetables, often termed as High Value Products (HVPs), are in greater demand of processing (Sanal & Krishna, 2017). The demands for processed products are particularly strong in developing countries like China and India; mainly due to increasing industrialization and rapid urbanization, followed by elevated income levels, greater diversity in diets, and affinity towards premium-branded products (Sharan, 2018).

Milk is a highly perishable commodity. The shelf life of raw milk is in hours. In order to harness its full potential for the well being of the humanity, it is essential to extend its shelf life not only in days but also in months and years. This can be achieved by judicious application of processing and preservation technologies.

Preservation is any purposeful treatment or processing of a food which prevents spoilage and extends the shelf life of raw material to a longer than its natural keeping quality.

During storage and distribution foods are exposed to a wide range of environmental conditions. Environmental factors such as temperature, humidity, oxygen and light can trigger several reactions that may lead to food deterioration. As a result foods may be altered to such an extent that they are either rejected by the consumer or are harmful to the person consuming them. It is, therefore, essential that the good knowledge of different reactions that cause food deterioration is gained prior to understanding the principles of food preservation.

The deterioration in foods may be caused either due to internal or external chemical or microbial reactions occurring in milk. The changes may be either latent to senses of consumer, or they may be discernible by the human senses. The consumer cannot perceive latent changes visually, olfactorily or otherwise, and laboratory measurements are the only method to detect them. Still the nutritional value of such materials may be seriously impaired. Such changes include fermentation, oxidation of lipids, loss of vitamins and changes in the contents and composition of nitrogenous substances.

Non-microbial changes in foods, perceptible to human senses, may often lead to serious deterioration noticeable to the average consumer. These include discolouration and changes in odor, taste and consistency. Microbial changes may result in alteration of original properties of milk making it unfit for further processing, deterioration in flavour quality, loss of nutrients and toxin production.

Yogurt is a fermented milk product that contains the characteristic bacterial cultures such as Lactobacillus bulgaricus and Streptococcus thermophilus. All yogurts must contain at least 8.25% solids not fat.

Introduction

Consumers are nowadays very much concerned about the food they purchase and consume. Consumer wants the food containing antioxidant properties or can say functional food in safe and stable forms, so that the availability of daily requirements of functional food ingredient must be ensured for normal growth and development of the body. The functional food that can provide a health benefit such as increased dietary fibre, vitamins or minerals to the body. The incorporation of antioxidant compounds acts as an efficient way to fight against reactive oxygen species (ROS) generated in our body. As it is already known that the ROS causes oxidative stress and acts as a main trigger for different life threatening chronic diseases like cancer, diabetes, cardiovascular disease, aging etc. Therefore, seeing the consumers’ demand for value added products with bioactive ingredients, all small and large scale food industries are taking initiatives to process different types of food (fruits, vegetables, dairy, sea food, animal, microbial products etc.) to extract and purify its active fraction of functional ingredients to valorize different value added products. Functional ingredients may also be derived from different food wastes generated during processing of food. This practice provides additional economic benefits to food businesses. Food industries are also giving importance to different processing methods to extract high quality of functional food ingredients in large quantity because processing method affects the concentration of extracted bioactive compounds. The simple addition of functional ingredients may cause an unwanted and negative change of sensory and texture of food. Therefore, using novel processing technologies such as microencapsulation, the negative changes could be minimized or avoided because these technologies could prevent the inactivation of bioactive ingredients.

Abstract

The objectives of the study is to help to create self-employment and provide proper information for different conventional milk based products. Traditional dairy foods have always played a crucial role in preservation of precious milk nutrients and promotion of its use among masses. The demand for milk and milk products is increasing with increasing of world's population. To meet these needs, high-end technology is needed for emerging the capability of milk production and preserving it by producing high quality milk products. The dairy industry is highly localized owing to perishable nature of milk products. Approximately 50% of the total milk produced in India has been projected to be processed into a variety of conventional milk products, such as khoa, chhena, paneer, dahi, ghee, kalakand and a wide range of sweets in rural areas mostly by the rural citizens. Therefore, setting up a milk-based food production system and updating conventional technologies to manage milk-based products on a commercial scale is very important.

5.1 Introduction
The process of milk processing entails the transformation of liquid milk into dairy products, including pasteurised liquid milk, yoghurt, butter, cheese, and ghee. The following are the rationales for processing: There are numerous benefits to processing milk, including the ability to access distant markets, the extension of the product’s storage life, and the attraction of higher prices because of its enhanced transportability. Furthermore, processed products are less cumbersome and lighter, which facilitates transportation. The refining of milk improves its quality and hygienic safety, enabling the production of a variety of milk products, including cheese and liquid milk, to satisfy the diverse needs of consumers. Additionally, the processing industry contributes to economic development by creating employment. Small-scale processors are capable of producing a wide range of dairy products. Before determining which dairy products the group will produce, it is recommended that a comprehensive market/feasibility study be conducted. Milk products can be processed in the manner depicted in the image.

1. Introduction

Product development is a basic activity in the food industry. Each company has a product mix, often including hundreds of products, which are constantly evolving - old products dying, products reaching maturity, products contributing to rapid growth and new products being introduced.

In the food industry, just as any other industry, product and process development is considered a vital part – indeed the lifeblood – of smart business strategy. Failure to develop new and improved products relegates firms to competing solely on price which favours the players with access to the lowest cost inputs (land, labour etc). Product and process development (commonly referred to as product development) is systematic, commercially oriented research to develop products and processes satisfying a known or suspected consumer need. Product development is a method of industrial research in its own right. There are essentially four basic stages in the model product development process.

Nutraceutical is a broad term which represents any substance extracted from food sources with additional health benefits alongside the good nutritional value already present in them. The nutraceutical term was originated by Stephen De Felice in 1989.

Definition
Production and operations management is the process of conversion of inputs into outputs, using physical resources, so as to provide the desired utility/utilities —of form, place, possession or state or a combination thereof—to the customer while meeting the other organizational objectives of effectiveness, efficiency and adaptability. It distinguishes itself from the other functions such as personnel, marketing etc. by its primary concern for ‘conversion by using physical resources’. Figure 1.1 describes a generalized concept of production system. It takes resource inputs and processes them to produce useful outputs in the form of goods or services.

Previous Year Questions

1. Write down the constraints of fruit processing industry in India (10M, CSE 2023)

2. Enlist & Explain the issues & challenges in the growth of food processing industry in India (10M, CSE 2022)

3. Give a detailed account of constraints in sustaining the food-grain production in India. (10M, IFoS 2020)

4. What are the major constraints of food production in India (10 mark, CSE 2020)

5. Describe the food production constraints in Indian agricultural system. (15M, IFoS-2018)

6. Indicate the major constraints in food procurement and distribution. (10M, CSE 2017)

7. Discuss the procurement and processing constraints of food grain. (15M, IFoS-2017)

Profitability in the ice cream business depends on controlling costs and protecting value across the complete product journey. This chapter examines both backend and frontend profitability levers, including yield improvement, giveaway control, manufacturing efficiency, OEE, utilities, refrigeration, quality costs, procurement, channel economics, distributor performance, route profitability, freezer productivity, and return on investment. It emphasizes that high sales volume does not necessarily guarantee sustainable profitability. SKU economics, pricing discipline, working capital, cold-chain losses, stock ageing, and trade schemes must also be managed. The chapter presents a structured profitability-improvement approach that connects manufacturing discipline, commercial strategy, operational efficiency, and financial governance to stronger contribution, EBITDA, cash flow, and sustainable business growth.

Introduction

Biotechnology is defined as the ‘application of scientific and engineering principles to the processing of material by biological agents to provide goods and services’. The term ‘biotechnology’ is also used when a technique involving live organisms are employed to make or modify a product, improve plants or animals or develop microorganisms for specific uses. According to the definition of the Codex Alimentarius Commission (CAC 2001), modern biotechnology is defined as the application of (i) in vitro nucleic acid techniques, including recombinant deoxyribonucleic acid (DNA) and direct injection of nucleic acid into cells or organelles, or (ii) fusion of cells beyond the taxonomic family, that overcome natural physiological reproductive or recombination barriers, and that are not techniques used in traditional breeding and selection. Scientific developments of present world owe to processes and products of the field of biotechnology. Modern biotechnological advancements have played a major role in important branches of science such as agriculture, food processing, medicine and veterinary applications. Biotechnological interventions of the downstream processing applications include primary production areas namely breeding of crops and livestock, production of additives for food and feed and pharmaceuticals along with diagnostic tools. Inputs for food processing applications from biotechnology include biocatalysts such as enzymes, tracing food ingredients such as Genetically modified (GM) ingredients in end products of food and feed and food quality testing applications for food borne pathogens. Applications of modern biotechnology in form of genetically modified organisms have gained much importance in comparison to the downstream applications during food processing and food safety (Zhu, 2017). Food processing operations include transformation of harvested crop or animal produce into produce which are marketable with long shelf life and minimises waste and losses of raw produce in food chain and ensure their continuous availability in super market shelves. Food safety is an important area which assures of the food being safe for consumption. Food technological applications of modern biotechnology includes food enzymes that are important in various food processing applications in reactions involving lipids, carbohydrates and proteins, food fermentations to improve properties such as taste, flavour, texture, nutritional and shelf stability, food additives in form of bio preservatives, flavours, fragrances, genetically modified starter cultures, genetically modified crops, modern rapid diagnostic kits for food safety are discussed in detail.

Introduction of Food Industry

India is one of the major food producers in the world. The food sector contributes to about 28% of India's GDP. India stands at 1st position in the world for production of cereals, milk, livestock, banana and Mango, 2nd in producing fruits and vegetables and ranks amongst top 5 in producing rice, wheat, groundnut, tea, coffee, tobacco, spices, sugar and oilseeds. India's share in global production of fruits is 10% and vegetables are 13.7%. The current consumption of fruits and vegetables is approx Rs.2 lakh crores at current prices with an estimated growth rate of 11% per annum. The growth rate is higher than cereals and milk and comparable to meet consumption

Status of Food Processing Industry

The food processing industry in India is still in a sorry state. The rural population comprising 70% including small cities, consume less than 10% of the processed foods and vegetables, whereas 60% of the processed food is consumed in four major metropolitan cities and 30% in the state capitals and big cities. Another fact is that 40% of the processed food and vegetables produced in the country in terms of value are bought by institutional buyers like Hotels, Restaurants and Defense etc. The highest growth in domestic market has been in fruit drinks, tomato ketch up and Jams.

There is another fact that India is the largest milk producer in the world, however, organized industry accounts for less than 15% of the milk produce in India. It is estimated that there may be a total production of 1100 million tons of production of food products mainly food grains, oilseeds, sugarcane and fruits/vegetables during 2011-12 and leaving marketable surplus of 870 million tons.

Like other sectors of the economy food processing sector also suffers from certain constraints. The challenges for the food conservation, distribution and processing sectors are diverse and demanding, which need to be addressed to leverage the growth of agriculture and food processing sector. Following are the major challenges and strategies are suggested to overcome the challenges (Sakina, 2019).

6.1. Domestic Constraints in the Development of Food Processing Industry in India

Despite the significant economic potential of the FPI in India, it still faces massive domestic challenges, which include low level of processing, fragmented structure of the industry, consumer perception and lack of skilled manpower. Firstly, low level of processing means that at industrial level, less food is processed, as compared to household level. Most of the traditional food items like jams, jellies, pickles etc. are prepared at small manufacturing units or by households (Agarwal & Neogi, 2017).

Secondly, consumer perception with regard to processed food in India is not very optimistic and they consider that packaged food is not fresh, thus, leading to low consumption of processed food. Thirdly, food processing sector is still highly fragmented and unorganised. This led to the inefficacies in the sector and has failed to achieve the economies of scale (Agarwal & Neogi, 2017).

7.1. Challenges in Milk Quality

Demand for milk and milk products in India is growing very rapidly, owing to urbanization, customer convenience, and the change from loose to packaged milk products. At present, the dairy sector is rising at about 10-12 per cent annually. Based on population growth projections and an increase in urbanization over the next four decades, India is projected to need approximately 600 million tonnes of milk per year to meet the demand for milk and milk products. This means that India’s milk production needs to grow at around 3.2 per cent CAGR for the next 40 years (FICCI, 2020).

Main reason of poor quantity of milk is due to unhygienic and poor condition of animal farms and dairies. Even due to high demand in foreign country we cannot export such poor quality of milk product. Many times, Indian milk products get rejected in foreign nations due to unhygienic milking milk contains high number of microbial counts. Reasons behind the quality affected in India are due to poor health of animal, polluted water and food and Unclean surrounding in farm. So, it is necessary to consider all this challenges in improving milk production. Currently most of the milking animals are not screened on regular interval of time. So, there is lots of chances that some diseases can transmitted to human beings. Farmers use antibiotics and chemical drugs to increase the production of milk this will also affect the quality of milk. These issues can be avoided by regular screening of animals in animal husbandry and also maintaining history records of health, feed etc. Quality of feed should be checked regularly. There must be pollution control in the feed and water used in cattle farms (Bhagyalaxmi, 2020).