eChapters

Emerging preservation technologies are being developed to meet increasing expectations for food safety, nutritional quality, sustainability and longer shelf life. Greater investigation remains necessary to understand their effects, limitations and suitability for large-scale industrial application. This chapter examines innovative physical, chemical and biological methods used at different stages of the food chain. Technologies include plasma-activated water, cold atmospheric plasma, advanced oxidation, electrolysed water, space-food preservation, hurdle systems and biopreservation. Their mechanisms involve reactive species, microbial membrane damage, oxidation, combined environmental stresses and beneficial biological agents. The chapter assesses their applications, advantages and disadvantages while highlighting research, regulation, process standardisation, equipment cost and commercial scalability.

Introduction

Food fraud is not new it can be traced back to ancient Rome and Athens, where regulations prohibiting the adulteration of beverages with flavours and colours were in place. It exists since the food trade has been started. Food fraud affects all food supply chains, thus the entire food sector. Due to globalisation and the increasing complexity of supply lines, food supply chains are becoming more vulnerable to food fraud (Robson et al., 2021a). A fraud is “any act or omission relating to the use or presentation of false, incorrect, or incomplete statements or documents, or non disclosure of information in violation of a specific obligation, and any other illegal activity affecting the financial interests” (EU No. 2017/1371).

Introduction

All the worldwide cuisines generally rely on plant-based edible oils as the main cooking ingredient due to their unique chemical composition, further presence of unsaturated fatty acids in plant-based oils makes it a healthy complement in the diet. Lipids are known to provide almost double calories than carbohydrates. For ages, vegetable oils have contributed maximally to our nutrition and economics, but their authenticity is still a considerable issue (Firestone and Reina, 1996) as for more profitability fraudulent blends lower grade oils to high-grade expensive oils.

14.1 Introduction

The increasing human population and changing life styles have resulted in a huge demand for processed and packaged foods. As a result of this demand, large amounts of water, air, electricity and fuel are consumed on a daily basis for food processing, transportation and preservation. Although not one of the most heavily polluting, the food industry does contribute to the increase in volume of waste produced [1]. There is an increasing pressure from regulatory bodies through incentives, legislation and financial penalties to minimize waste generation and encourage adoption of eco-friendly options for waste management. Nanotechnology is a panacea for food waste management.

Employee motivation and job enrichment are described as two management techniques used to improve human behaviour and attitude towards work, with a view to utilize available human resources more efficiently and thus make man-management more effective. Just as the employee has certain wants that the organization is expected to satisfy, the organization too expects certain types of behaviour usually termed as ‘Direction’ or ‘Motivation’. Obviously this managerial function is not so easy, as it involves many problems, and it is these problems and their possible solutions that one is concerned.
Job enrichment is also a motivational problem created by the alienation of employees from their work, or by lack of their interest in their work. Here the problem is how to make the work more interesting, purposeful and acceptable to employees so that they may perform it more enthusiastically and with a greater sense of responsibility. Like motivation this is also a problem of human behaviour and work attitude. In fact both employee motivation and job enrichment are the problems which every supervisor and manager has to face while working and making their subordinates work.

Probiotics have been newly defined as ‘live microbes which passage the gastrointestinal tract and in doing so benefit the health of the consumer differing from the earlier definitions which focused on probiotic interfaces with original intestinal microbes (Fuller, 1989). These definitions of probiotic bacteria generally agree that probiotic bacteria should be living organisms toward consult health benefits. Probiotics have been reported to play a therapeutic role by modulating immunity, lowering cholesterol, improving lactose tolerance and preventing some cancers (Kailasapathy and Chin, 2000).

1. Introduction

Energy balance means keeping an account of how the flow of energy is taking place in and out of any system or process. It helps the engineers to estimate the total energy requirement and identify the area for conservation of energy in any process. In food processing the concept of energy balance is applicable in unit operation such as blanching, drying, evaporation, freezing, refrigeration, distillation, crystallization. The scopes of energy balance in food industry are:

i) Streamlining and reduction of energy consumption in the existing process.

ii) Selection and sizing of any equipment and process.

iii) Economic analysis of alternate process/technology.

iv) Designing new economically viable process/technology

Introduction

Over the years, processed food industry has become the largest growing industry in the world. The convenience food industry with the state of the art technology has been proved very successful in reaching every household and have started replacing fresh and healthy foods with the processed ones (Srinivasan and Shende, 2016).

Demand for processed and convenience foods are constantly on rise and convenience food segment is rising by leaps and bounds. Changing life style, food habits, displacement of rural population to urban centres, insufficient time to prepare healthy food and changing socio economic needs have led to the industrialization of the food supply and resulted in the development of convenience foods and today a great variety of the same are available for sale to the consumer throughout the world (Tillotson, 2003).

These convenience foods are available readily, convenient, inexpensive with pleasing organoleptic quality, requires minimal or no preparation before consumption. Varieties of convenience foods which are available in the market play a prominent role in the food choice of today’s consumer, and they include frozen and canned vegetables, bakery products, soups, sauces, prepared meat and fish, chilled and frozen dairy products as well as ready to eat and shelf stable products (Tillotson, 2003).

Rapid switchover of the consumer from the traditional dining practices to fast food centres, generally feed on quick and easily made products called fast foods, often termed as unhealthy junk foods. These foods are rich in saturated fatty acids, several refined components, high carbohydrates etc., which ultimately lead to the negative impact on physical and mental health of the consumer, contributing to several chronic diseases such as cardiovascular complications, cancer, atherosclerosis, liver disease, type 11 diabetes (Veggie et al., 2018) etc.

Therefore, focussing much about the health of the consumer, there is a great need for a healthy and a balanced diet to meet the basic needs of the human body (Mendes et al 2013) with an appealing organoleptic properties and appearance.

1. Introduction

1.1 Engineered health rice

Rice is one of the leading food crops in the world and sustains two-third of the world’s population providing 20% of the world’s dietary energy supply. It can be polished and marketed as white rice or can be marketed with the bran layer left intact as brown rice. The dry grain can then be steamed or boiled by the consumer to rehydrate and cook it (Kato, 2006). Despite its primary importance as a staple food, rice has several disadvantages. Primarily, rice is low in protein and high in starch. The low protein levels in rice can lead to protein malnutrition in societies where rice is the primary constituent of the diet. Deficiencies in essential amino acids may also result from a diet primarily comprised of rice. For example, lysine is the essential amino acid found in the lowest quantity in rice. The human body needs lysine for proper growth and repair of tissues. It is also important in the production of various enzymes, hormones and disease-fighting antibodies (Kato, 2006). The production of broken rice is nearly 15-20% of total production of rice (Bhullar and Bhullar, 2013). The broken rice is generally used for rice flour production and Indian traditional foods such as Idli, Dosa and weaning foods etc. Ugwu et al., (2012) reported that the rice broken can be utilized for the production of poly (3-hydroxybutyrate). The major value addition of broken rice is possible by reconstructing it into rice-shape and fortifying it with desired nutrients or micronutrients, which is a big challenge in terms of making the product acceptable to the consumers.

One approach to overcome the above mentioned challenges is to prepare artificial rice kernels or rice analogues by extrusion, in which the vitamins are embedded and consequently do not separate from the rice grains. This will utilize mainly the broken rice which is either discarded or sold at subsidized prices as the cattle feed. Furthermore, embedding makes extraction of the vitamin by rinsing or cooking more difficult and may provide a certain protection against oxidation, because the vitamins can be enveloped by a protective matrix (Steiger, 2010). The fortification of commercially produced staple foods allows exposed populations to benefit without making major changes to their eating habits (Kunz, 2009).The rice analogues prepared from extrusion can be used by addition of an appropriate proportion of water at a temperature ranging from 90°C-100°C, whereupon the rice product rehydrates within three minutes to obtain a product having the desired appearance and organoleptic properties to simulate natural rice grains (Harrow and Martin, 1982).

Introduction

Entrepreneurship has become a major driver of economic development in recent years. It involves starting new businesses or revitalizing existing ones, often through recognizing and seizing new opportunities. Entrepreneurs are individuals who organize, manage, and take on the risks of a business venture with the aim of making a profit. They identify market opportunities and introduce new technologies and organizational improvements. Entrepreneurs constantly innovate and create value from these opportunities. According to Hisrich (1990), entrepreneurs are proactive and creative individuals who can organize resources and manage risks and failures to succeed.

To grow as an entrepreneur, it is essential to develop and refine key entrepreneurial traits. These traits shape an entrepreneur’s skills and abilities, aligning with their motivations and guiding them to drive their business forward. Entrepreneurial characteristics are a mix of personal, social, and environmental factors that influence a business ecosystem. These traits play a key role in shaping a company’s goals and strategies, ultimately affecting its performance.

Introduction

Entrepreneurship, known for its ability to drive innovation, economic development, and societal change, is a dynamic force that plays a crucial role in global advancement. However, the path of an entrepreneur is not a straightforward journey from concept to achievement. Instead, it is a complex process influenced by numerous factors, which together form the entrepreneurship development cycle. Gaining a deep understanding of this cycle, along with the entrepreneurial environment and the challenges entrepreneurs face, is essential for cultivating a thriving entrepreneurial ecosystem.

The entrepreneurship development cycle refers to the various stages that entrepreneurs go through as they turn ideas into successful ventures. The cycle starts with ideation, where entrepreneurs identify opportunities and develop innovative solutions to address unmet market needs or problems. This is followed by a feasibility assessment, during which entrepreneurs assess the potential of their ideas, considering market demand, competition, and the resources needed. After confirming the viability of their concepts, entrepreneurs move on to resource acquisition, gathering the capital, talent, and infrastructure necessary to bring their ideas to life. The subsequent phases include launching the venture, establishing a market presence, and pursuing growth through strategic decision-making and adaptability to shifting market conditions.

Introduction

Entrepreneurship is crucial for economic development, especially during crises when job losses push people to find alternative livelihoods, often turning to entrepreneurship as a means of survival. Its impact is highly beneficial, promoting growth, wealth creation, and better living standards. In developing countries like India, entrepreneurship offers a path to holistic development, particularly for disadvantaged groups traditionally dependent on agriculture. Uplaonkar and Biradar (2015) noted that entrepreneurship development effectively generates employment, increases income, reduces poverty, improves health, and ensures food and nutritional security. Recognizing the importance of entrepreneurship, stakeholders in the agricultural sector now see its positive impact on business sustainability. Verhees et al. (2011) argued that promoting entrepreneurship in agriculture can significantly reduce poverty and boost rural prosperity, directly addressing socio-economic challenges in rural areas (Bhooshan and Ranjith, 2022). Therefore, supporting the entrepreneurial ecosystem in rural sectors through programs and schemes is essential.

Introduction
This method is used to determine the ‘total bacterial count’ (living and dead  organisms) by direct microscopic examination in samples of broth culture, cell suspension and milk. Simple and Gram’s staining techniques are used to stain the cells. This method is simple and a rapid. A known volume (0.01 ml) of the sample is spread over a known area (1cm2) on a glass slide. The sample is allowed to dry; then stained and examined microscopically. The average number of cells per field is determined and is then calculated by applying microscopic factor.
Materials
1. Microscopic slide with one sq. cm. area marked
2. Breed’s pipette (0.01 ml)
3. Methylene blue or Newman’s stain
4. Culture of E.coli or Bacillus subtilis
Procedure
1. Measure the diameter of microscopic field with a stage micrometer using oil immersion objective and 10X eye piece.
2. Area of field πr2.
Determination of microscopic factor (MF)

(A) Enumeration of Microbial Growth by Turbidity Measurement
Method and Estimation of Generation Time
Introduction
Bacterial growth usually refers to reproduction-increase in population sizerather than to enlargement of mycelia cells. “Reproduction in bacteria occurs mainly through binary (binary means one cell becomes two) or transverse fission that results in the formation of two daughter cells. This is repeated at intervals by each new daughter cell in turn, or with each successive round of division. The population increases (Fig. 1). The time required for a complete fission cycle i.e. parent cell to two daughter cells is called the generation or doubling time. In bacteria, it is a doubling process in which each new fission cycle or generation increases the population by a factor of 2. So the parent stage consists of 2 cells, the second of 4, then 8, 16, 32, 64, 128, 256 and so on (Fig. 2). Thus the population will double, over and over again as long as the environment remains favorable for growth. The length of generation time is a measure of the growth rate of an organism. The average generation time in bacteria is 30-60 minutes under optimum conditions. Some species have very rapid growth rate and short generation time (5-10 minutes) and others have very slow growth rates and long generation times (measured in days). Staphylococcus aureus and Salmonella enteritidis, both causes of food poisoning, are examples of pathogens having relatively short doubling times of 20-30 minutes, which result in a few million cells from a few cells in a few hours in the infested food. The longest generation time occurs in species of Mycobacterium, particularly Mycobacterium leprae (the cause of leprosy) of 10-30 days as long as in some animals.

1. The Aflatoxin Molecule

Aflatoxins are chemically difuro-coumarin derivatives. Presently, 18 different types of aflatoxins have been identified with aflatoxin B₁, B₂, G₁, G₂, M₁ and M₂ being the most common. Of these Aflatoxin B₁ (AFB₁) and G₁ occur most frequently, with AFB₁ being the most potent. Physicochemical and biochemical characteristics of the AFB₁ molecule reveal two important sites for toxicological activity (Heathcote and Hibbert, 1978). The first site is the double bond in position 8,9 of the furofuran ring (Figure 1). The interactions of aflatoxin, DNA and proteins, which occur at this site alter the normal biochemical functions of these macromolecules and leads to deleterious effects at the cellular level.Monteiro et al. (1966) studied the in vitro interaction of groundnut proteins namely arachin and conarachins with AFB₁ molecule. The second reactive group is the lactone ring in the coumarin moiety (Lee et al., 1981). The lactone ring is easily hydrolyzed and is therefore vulnerable to degradation.

Introduction

The entire living organism from the smaller creatures viruses to the advanced human beings are composed of a set up of biochemical reactions which form the metabolism. These set of metabolic reactions are responsible for the life of organism. The rate of these reactions is a major concern for effective working of organism which is governed by biocatalyst. These biocatalysts are known as enzymes, which accelerates rate of biochemical reaction. These are not utilized in reaction. In 1877, Wilhelm Friedrich Kuhne (Kuhne, 1877) proposed the term enzyme which means ‘in yeast’ and is derived from the Greek word; inzume “leavened” or “in yeast”. At the molecular level of study it is observed that enzymes are proteins which are functional as tertiary and quaratanary structures. Enzymes have active site for the binding to substrate. The active site has two positions; binding and catalytic site (Ogeston, 1948). The former help to bind the substrate with the enzyme followed by the catalysis of reaction by the catalytic site.

The establishment of a technology transfer office is an important and complex process for an organization. It needs careful planning to ensure its long-term sustainability. Before establishing a TTO, it is essential to evaluate whether institution’s core mission includes research commercialization, and if the volume and quality of its research output justify the need for an inhouse TTO. For institutions that may not meet all criteria, those institutions can collaborate or outsource for TTO services. It is also important to decide about its operational scope, funding, management, and for what kind of issues the office should develop a policy. There is no “right” way to set up a TTO, but successful TTO does require considering some key issues. This chapter outlines key steps and considerations for establishing a TTO. It also explains what physical infrastructures are needed, manpower, outlines the responsibilities and powers of TTOs.
2.1 Evaluating the need for a TTO
Establishing a TTO is an important step for any research focused organization. But before initiating a planning process for a new TTO, the organization must review its relevance by considering a few fundamental points:
Key considerations for institutions
1. Is institution’s mission focused on “research commercialization” If an institute’s primary mission is education, or it does not support research it might not be justified to create a TTO. For such institutions, it usually makes better sense to seek alternative solutions to the occasional technology transfer without the expense of a formal office. But institutions with strong research commercialization focus, a TTO has the ability to contribute meaningfully to the mission. The Association of University Technology Managers (AUTM), USA has identified following key motivations for initiating academic technology transfer:

Performance metrics and indicators
Traditionally, in the past, TTOs’ performance was measured in monetary terms, such as universities’ revenues derived through commercialization. The methodology, however, is prone to shortcomings, in light of the fact that various TTOs do not receive significant amounts of financial proceeds through their technology transfers. In addition, relying on income does not depict a TTO’s true potential, in light of the fact that it does not account for the total range of outputs of research and commercialization opportunities. Recognizing these limitations, academics have found innovative ways to measure TTO efficiency, such as monetary and non-monetary indicators. Financial indicators typically entail measurable outcomes such as number of signed licenses, income from licenses, royalties collected, equity income, and number of spin-offs or startups created. These are actual gauges of the economic returns from commercialization efforts, and in the majority of instances are used in the context of benchmarking the performance of a TTO in a comparative context, perhaps against peer institutions or industry averages. Non-financial indicators consist of royalty distributions to academics, having an integral role in encouraging academics to participate in commercialisation activity. By incorporating monetary and non-financial metrics, universities might have an enhanced, comprehensive evaluation system for their TTOs. Such evaluation is sure to ensure assessment extends beyond short-run monetary metrics and looks to incorporate several organization, institutional, and research-oriented determinants in driving successful technology transfer.

1. Introduction

Foods available in nature and, in some cases, the products made from it thereof contain quite an amount of water. This water not only adds volume to the food products but also provides a sight for attack by the micro organisms. Also, removal of water becomes essential if this water interferes with the end product chracteristics. There are several processes in food industries which are designed to get rid of this excess water, such processes are called concentration processes. The processes which can remove water from food substances are,- evaporation, freeze concentration and membrane based processes. All these process have their limitations in rendering solid content to the final product (Fig. 1). Perhaps, these limitations can be overcome by combinig the processes.

The objectives that are fulfilled by the concentration processes can be enumerated as follows:

7.1 Introduction

Evaporation is an unit operation which converts a liquid into a vapour, leaving behind a concentrated solution or a higher boiling liquid. In other words, the goal of evaporation is to vaporize most of the water from a solution containing a desired product. Heat is the main tool in evaporation, and the process occurs more readily at high temperature and low pressures. Removal of water from foods provides microbiological stability, reduces deteriorative chemical reactions, and reduces transportation and storage costs. Evaporation can be distinguished from some other drying methods in that the final product of evaporation is a concentrated liquid, not a solid. It also differs from distillation, since the vapours produced in the evaporator are not further divided into fractions.