eChapters

1. Introduction

Fruit products have their unique identities having large content of micronutrients, polyphenols, vitamins, flavonoids and other nutritional compounds. Characteristic appearance and flavors distinguish among various fruit based products like juice, puree, jam, jelly, squash and other several varieties. Main concern of this fruit processing industry is the stability of the products throughout the post processing storage. Thermal treatment as mean of preservation of these products is the common process implemented so far. It ensures the safety of the product but lacks in retaining the nutritional intact that like of fresh fruit. Today’s consumer demand for minimally processed fruit products and “natural” fruit products tend to think of alternative thermal processing.

High pressure processing (HPP), a relatively new concept compared to conventional thermal processing, also sometimes known as high hydrostatic pressure (HHP), or ultra high pressure (UHP) processing, is a non-thermal food processing method that subjects liquid or solid foods, with or without packaging, to pressures ranging between 50 and 1000 MPa. HPP has already been established itself as an alternative to thermal processing. In food processing it is of great interest due to its ability to inactivate food borne microorganisms and enzymes, at low temperature, without the need for chemical preservatives and it is less detrimental than thermal processes to low molecular weight food compounds such as flavoring agents, pigments, vitamins, etc., as covalent bonds are not affected by pressure. Pressure-treated foods have sensory properties similar to fresh products, which is a major advantage in fruit processing as it matches consumer demand for healthy, nutritious and ‘‘natural’’ products. HPP of fruit products offers the chance of producing food of high quality, greater safety and increased shelf life (Hayashi 1989; Gould 1996; Butz and Tauscher 1998).

1. Introduction

The demand for non-dairy based beverages in form of non-dairy milk is on rise due to several reasons. Firstly, the prevalence of lactose intolerance and milk allergy amongst a major percentage of population who cannot consume dairy milk and look for alternate source of proteins. The need for functional drinks is also a major factor which contributes to the increase in demand of milk-alternatives. It has also been reported that milk processors around the world face an uphill battle, including the dominance of private labels; consumer concerns over the presence of growth hormones in non-organic milk; fluctuations in commodity prices; supply shortages for organic milk; and growing consumer concern about the safety of animal products in general. With that long list of challenges, the market has had few growth opportunities even though consumers continue to prize milk for its nutritional benefits.

2. Need for Non-dairy Beverages

2.1 Prevalence of lactose intolerance

Lactose intolerance is estimated to affect 33 % of the global population, but the prevalence of the condition varies from country to country. It is also estimated that an average of 75 % of human adults have decreased intestinal lactase activity after weaning (NDDI, 2012).

1. Introduction

There is a general consensus that fruit and vegetable consumption is a key factor for a balanced diet. Globally, majority of people consistently are consuming less than the daily recommended fruit and vegetable requirement. Even in developed nations e.g., Australia, Canada, Europe, UK and USA, researchers have concluded that there is large gap between actual and recommended consumption of both fruits and vegetables despite decades of concern and publicity while resultant outcomes were short-lived. In a study from 52 low and middle-income countries 77.6% of men and 78.4% of women consumed less than the minimum recommended servings of fruits and vegetables. Same study reported 74% low fruit and vegetable consumption among adults in India (Hall et al., 2009). In India, recent studies on the fruit and vegetable intake amongst urban residents was reported as 265 g per day as against 400 g per dayof fruits and vegetables excluding potatoes and other starchy tubers, that is recommended by the World Health Organization (WHO, 2008).

Epidemiological studies conducted in recent years have indicated a positive correlation between the consumption of fruits and vegetables and prevention of cancer and cardiovascular diseases. Health-promoting functional characteristics of vegetables are mainly attributable to their functional bioactive constituents. These effects can be listed as antioxidant activity, cholesterol-bindingcapacity, antidiabetic activity and angiotensin-converting enzyme (ACE) inhibitory activity. Studies conducted in cell culture models and animal models have revealed that bioactive compounds of plant foods, including soybean, hawthorn fruit, tea, buckwheat, oats, onion, garlic, almond, and vegetable juice, composed of tomato watercress, parsley, carrots, celery, lettuce, beets and spinach, have cholesterol-lowering activity (El-Shatanovi et al., 2012; Vadivel et al., 2011).

1. Introduction

Now a days, foods are not intended to only satisfy hunger and to provide necessary nutrients but also to prevent nutrition-related diseases and improve physical and mental well-being of the consumers (Menrad, 2003). In this regard, functional foods play an important role. The concept of functional foods which has evolved as the role of food in the maintenance of health and well-being and in the prevention of disease, has received increased scientific and commercial interest is recent past. Growth of functional foods markets include increased consumer health awareness, increased popularity of healthy foods, search for food-based drug substitutes, self-medication approach promoted by busy lifestyles and rapidly developing health conscious markets. Functional foods can be defined as foods “that can beneficially affect one or more target functions in the body, beyond adequate nutritional effect, in a way relevant to an improved state of health and wellbeing and/or reduction of risk of disease” (Stanton et al., 2005).

1. Introduction

Milk and milk products constitute essential dietary components for a vast majority of the population. India with annual production of more than 135.6 million tonnes has emerged as leading milk producing nation. The per capita per day availability of milk in India is more than 290 g. Buffalo milk accounts for 57% of the total milk produced in India. Ethnic dairy products account for 90% of all dairy products consumed (Aneja et al., 2002). Many health benefits have been attributed to fermented dairy products and probiotic microorganisms (Salminen et al., 1998). The market for functional foods, nutraceuticals, wellness foods and beverages is fast growing. The harmful effects of synthetic drinks have propelled health conscious consumers to go for natural and traditional drinks. In search of better returns, the Indian dairy industry is widening its focus to include traditional milk products, and these are emerging as new profit centres for the organised sector.

Traditional dairy products have several advantages. They require low capital investment and give high profit margin. They are more appealing to the Indian palate. Dahi or curd is the most popular indigenous fermented dairy product. There are numerous references to dahi in vedic literature. The Prevention of Food Adulteration Act (Anon, 1976) describes dahi as a product obtained from pasteurized or boiled milk by souring (natural or otherwise), using harmless lactic acid or other bacterial cultures. Indian dahi and its western counterpart yoghurt differ considerably in terms of cultures used and their bouquet and flavour profiles (Aneja et al., 2002). The major flavor compound in dahi is diacetyl. Dahi made from buffalo milk has a firm body and white glossy appearance, with a cream layer on top. Mixed mesophilic cultures of Lactococcus lactis subsp. lactis, Lactococcus lactis subsp. cremoris, Lactococcus lactis subsp diacetylactis along with Leuconostoc species are grown together for production of dahi.

1. Introduction

Fermented milk products such as yoghurt are produced through out world (Kurmann et al., 1992). Yoghurt can be characterized as a gel like coagulated milk product, having a smooth consistency and a pleasing flavour and is now widely consumed both by infant and adult populations. It is common that yogurt preparation involves the use of equal amounts of two lactic acid bacteria (LAB): Streptococcus thermophillus (St) and Lactobacillus delbrueckii ssp. bulgaricus (Lb); samples prepared with these starter bacteria are labeled Lb-St (Ng et al., 2011). In the early stage of the incubation process, Lb stimulates the growth of St releasing amino acids from casein (where the most important is valine) and producing a moderate reduction in pH due essentially to St. Yoghurt has better digestibility than milk and this is related to its comparatively high content of free amino acids. Besides nutritional value, it possesses hypocholesterolaemic (Mann, 1977) and antitumour effects (Odd et al., 1983; Reddy et al., 1983).Yoghurt may be set or stirred and has a potential for being used as dietary supplement for infants. So they cross the line between dietary supplements, health foods and conventional foods with great facility. Now a days, emphasis is given on sweeteners and more natural sweeteners will be used (Hollingsworth, 2001). Natural sweeteners can be easily incorporated in yoghurt by blending cattle milk with fruit pulps like mango. The enhancement of other nutrients e. g. vitamins and minerals will be added advantage. Mango pulp which contains a high proportion of sugar, mostly monosaccharide, is easily adsorbed by the digestive systems (Celik and Bakirci, 2003).

Soybean is a comparatively inexpensive source of high quality protein. The protein and fat contents in soybean are above 40 % and 20 %, respectively. Nutritionally, soy protein is a more balanced plant protein for human consumption. Studies have indicated that consumption of soy protein decreases total serum cholesterol and minimizes risks for several cancers (Anderson et al., 1995; Messina, 1997; Zind, 1998). The positive health benefits associated with soybeans have greatly increased its consumer awareness which has created a large market for soy foods (Ohr, 2000; Liu, 2000). The problem of beany flavour in soy products can be overcome by lactic acid fermentation (Buono et al., 1990; Granata and Morr, 1996). Soy solids are not only abandoned source of protein but also have therapeutic properties against breast, prostate, colon and lung cancers due to its isoflavones like genistein and daidzein content. Fortification with soy made yoghurt beneficial in lowering cholesterol level and blood pressure and in relieving symptoms of menopause and osteoporosis.

1. Introduction

Extrusion is one of the most preferred ways for creating the indulgence called ready-to-eat (RTE) snack foods with diverse shapes, sizes, colours and also nutritional and functional values which appeal to the diverse group of consumer with varied choices. In this chapter ready-to-eat extruded and puffed snacks are the focus covering the technology involved, criteria for ingredient selection, design of experiment, process modelling, effects of extrusion on physical, nutritional and functional quality of the snack; a few relevant case studies are also discussed.

1.1 Market for RTE snack foods

Indian cooking and lifestyle have undergone tremendous changes in the last 15 years. There are many major factors impacting this change which include, liberalization policy, dual income, separate living of couples, innovative kitchen applications, media proliferation, etc. The cooking style and eating habits in India varies drastically from southern part to northern part of the contry. Due to lifestyle pressure nowadays people prefer easy short way of cooking food rather spending too much time on cooking. Rapid urbanisation is also one of the reasons for people to buy such products. Snack foods comprise a very large variety of items including potato chips, crackers, nuts and extruded snacks, among others (Harper, 1981). Snack food extrusion includes subjecting selected raw material to a variety of complex physical processes to yield snacks with varied shapes and textures (White, 1994).

The RTE market in India was expected to expand to reach Rs. 2,900 crore by 2015, according to an analysis done by Tata Strategic Management Group (TSMG). The products may vary from noodles, pasta, masala packages, stuffed paratha, etc. and many more. There are many regional players who are making foray into ready-to-eat segment. Many national and international players like Nestle, Pepsico, ITC, Haldirams, Cavinkare, are already in Indian market with their products.

1. Introduction

1.1 Malnutrition

The term malnutrition generally refers both to under nutrition and over nutrition, but in this guide we use the term to refer solely to a deficiency of nutrition. Many factors can cause malnutrition, most of which relate to poor diet or severe and repeated infections, particularly in underprivileged populations. Inadequate diet and disease, in turn, are closely linked to the general standard of living, the environmental conditions, and whether a population is able to meet its basic needs such as food, housing and health care. Malnutrition is thus a health outcome as well as a risk factor for disease and exacerbated malnutrition, and it can increase the risk both of morbidity and mortality (Ogunrinade, 2014). A term used to refer to any condition in which the body does not receive enough nutrients for proper function. A person cannot properly digest or absorb nutrients from the food they consume, as may occur with certain medical conditions (Medicine, 2015). Malnutrition may range from mild to severe and life-threatening. It can be a result of starvation, in which a person has an inadequate intake of calories, or it may be related to a deficiency of one particular nutrient (for example, vitamin C deficiency).

Malnutrition is estimated to contribute to more than one third of all child deaths, although it is rarely listed as the direct cause. Lack of access to highly nutritious foods, especially in the present context of rising food prices, is a common cause of malnutrition. Poor feeding practices, such as inadequate breast feeding, offering the wrong foods, and not ensuring that the child gets enough nutritious food, contributes to malnutrition (WHO, 2015).

Pooled estimates report the prevalence of malnutrition among community-dwelling older adults of 6% and among hospitalized older adults of 39%. In developed countries, treatment of malnutrition is relatively inexpensive and effective: energy and protein supplementation using commercial “sip feeds” results in both weight gain and reduced mortality (Pereira et al., 2015). In 2013, one in four children under age five worldwide had stunted growth. Between 2000 and 2013, stunting prevalence globally declined from 33 to 25 %, and the number of children affected fell from 199 million to 161 million. In 2013, about half of all stunted children lived in Asia and over one third in Africa (UNICEF, 2015). Malnutrition may be several types as follows:

1. Introduction

1.1 Engineered health rice

Rice is one of the leading food crops in the world and sustains two-third of the world’s population providing 20% of the world’s dietary energy supply. It can be polished and marketed as white rice or can be marketed with the bran layer left intact as brown rice. The dry grain can then be steamed or boiled by the consumer to rehydrate and cook it (Kato, 2006). Despite its primary importance as a staple food, rice has several disadvantages. Primarily, rice is low in protein and high in starch. The low protein levels in rice can lead to protein malnutrition in societies where rice is the primary constituent of the diet. Deficiencies in essential amino acids may also result from a diet primarily comprised of rice. For example, lysine is the essential amino acid found in the lowest quantity in rice. The human body needs lysine for proper growth and repair of tissues. It is also important in the production of various enzymes, hormones and disease-fighting antibodies (Kato, 2006). The production of broken rice is nearly 15-20% of total production of rice (Bhullar and Bhullar, 2013). The broken rice is generally used for rice flour production and Indian traditional foods such as Idli, Dosa and weaning foods etc. Ugwu et al., (2012) reported that the rice broken can be utilized for the production of poly (3-hydroxybutyrate). The major value addition of broken rice is possible by reconstructing it into rice-shape and fortifying it with desired nutrients or micronutrients, which is a big challenge in terms of making the product acceptable to the consumers.

One approach to overcome the above mentioned challenges is to prepare artificial rice kernels or rice analogues by extrusion, in which the vitamins are embedded and consequently do not separate from the rice grains. This will utilize mainly the broken rice which is either discarded or sold at subsidized prices as the cattle feed. Furthermore, embedding makes extraction of the vitamin by rinsing or cooking more difficult and may provide a certain protection against oxidation, because the vitamins can be enveloped by a protective matrix (Steiger, 2010). The fortification of commercially produced staple foods allows exposed populations to benefit without making major changes to their eating habits (Kunz, 2009).The rice analogues prepared from extrusion can be used by addition of an appropriate proportion of water at a temperature ranging from 90°C-100°C, whereupon the rice product rehydrates within three minutes to obtain a product having the desired appearance and organoleptic properties to simulate natural rice grains (Harrow and Martin, 1982).

1. Introduction

Celiac disease (CD) is an immune-mediated enteropathy triggered by the ingestion of gluten in genetically susceptible individuals. Gluten is the protein component in wheat, rye and barley. In the last 10 years, cutting edge scientific developments in this disease have led to the formulation of new concepts of epidemiology, pathophysiology and clinical manifestations. At present, the only available treatment for CD is a strict gluten-free diet (GFD) (Mulder et al., 2013; Theethira and Kelly, 2014). Until recently, the geographical distribution of CD was mostly restricted to Europe and other developed countries, such as the United States, Canada and Australia. Celiac disease is not only frequent in developed countries, but it is increasingly found in areas of the developing world, such as North Africa (Malekzadeh et al., 2005), Middle East (Rostami et al., 2004) and India (Basu et al., 2014, Deora et al., 2014; Yachha and Poddar, 2007). New epidemiological studies have brought evidence that this disorder is common in many developing countries as well, showing that the ‘global village of celiac disease’ has indeed a worldwide distribution (Fasano and Catassi, 2001).

The only available treatment for celiac disease is the strict, lifelong avoidance of gluten. When gluten is removed from the diet, the small intestine will start to heal and overall health should improve (Fasano and Catassi, 2001). Traditional substitutes for gluten-containing grains have been rice, tapioca, corn, and potato. However, more nutrient-dense grains, seeds, nut flours, and legumes are being incorporated into the diet to provide an enhanced palatability, variety, and nutritional quality. These include sorghum, millet, teff, quinoa, amaranth, flax seed, and buckwheat.

1. Introduction

The development of food products using composite flour has increased and is attracting much attention from researchers, especially in the production of bakery products and pastries. This article focuses on the use of composite flour to produce food products, namely bread, biscuits, and pasta, with looks at on its impact, following some improvements made, on the sensory quality, rheology characteristics, and nutritional values as well as its overall acceptance. The blending of wheat flour with various sources of tubers, legumes, cereals and fruit flour in different percentages to produce variety of food products are also reported. The positive effects of the use of composite flour can be seen in the final product related to the functional and physicochemical properties and health benefits of raw blended flour along with percentage blending. Overall, composite flour is a good new approach to utilizing uncommon food products as the application of composite flour produced products with different characteristics and quality, depending on the types and percentage of wheat flour used in the formulation.

Bread has been the staple food worldwide and habit of bread is derived in part due to the convenience and wholesomeness. Wheat is a commonly used cereal for making bread as it gives the desired texture due to the presence of gluten forming proteins like gliadins and glutenins (Decock & Cappelle, 2005). In earlier times idea of composite flour in developing countries originated in order to cut down on import of wheat (as it was not grown in many countries) but in recent past refined wheat flour consumption has lead to health issues and thus in order to improve nutritional and functional properties composite flour has been the norm. Composite flour is the one obtained by mixing wheat flour with cereal and legume flours for making bread and biscuits (Dendy, 1992). However, mixing of non-wheat flours, roots and tubers, legumes or other raw materials can also be regarded as composite flour.

Over the past several years, there has been significant change in consumer perception on quality and food safety. Consumers are demanding healthy, nutritious and cost-effective products processed under controlled hygienic conditions. Food safety concern, in recent years, has become dominant and important. The requirement of food safety and quality assurance has never been so demanding as it is now. Globally food safety is being addressed independent of food quality. In anticipation of consumer demands on food safety, food sanitary standards at national and international levels are under constant review. Several inter governmental agencies/organizations are engaged in work related to quality assurance and food safety. Amongst these World Health Organization (WHO) and Codex Alimentarius Commission (CAC) play the major role in protecting public health and defining standards of quality and safety aspects of foods for regional and or world wide usage.

1. Tyson Foods Inc. 
2210 W. Oaklawn Drive, Springdale, AR 72762-6999 
Phone  : 479-290-4000; Fax  : 479-290-4061; Web site  : www.tyson.com

Executives : Chairman : John Tyson; Pres./CEO : Richard Bond; SVP-Intl. Ops. : Mike Baker; SVP-Commodity Trading & Risk Management : Jean Mrha Beach; Group VP-Research & Development, Logistics & Tech Services : Hal Carper; SVP & CIO : Gary Cooper; Group VP & International Pres. : Rick Greubel Jr.; SVP, Controller & CAO : Craig Hart; VP, Assoc. General Counsel & Secretary : R. Read Hudson; Group VP-Ops : Donnie King; SVP-Human Resources : Ken Kimbro; EVP & CFO : Dennis Leatherby; Group VP-Food Service : Bernard Leonard; Sr. Group VP-Fresh Meats : James Lochner; SVP-External Relations : Archie Shaffer III; Group VP-Consumer Prods : Donnie Smith; EVP & General Counsel : David Van Bebber; SVP-Renewable Products : Jeff Webster; VP-Investor Relations & Asst. Secretary : Ruth Ann Wisener

    1.    Cereals are grown over 73% of the total world harvested area and contribute over 60% of the world food production providing dietary fibre, proteins, energy, minerals,  vitamins, b-glucans , Oligosaccharides, such as lactulose, fructo-oligosaccharides, transgalacto-oligosaccharides

    2.    Finger millet is a potent source of antioxidants and has potent radical-scavenging activity that is higher than that of wheat, rice, and other millets; these results corresponded to their phenolic content

    3.    The brown or red variety of finger millet, which is commonly available, had higher activity (94%) than the white variety (4%) using the DPPH method.

    4.    Most of the herbal plant polyphenols are known to have multi functional properties such as reducing agents, hydrogen donating antioxidants and singlet oxygen quenchers and flavonoids and their derivatives are the largest and most important group of polyphenols.

    5.    Phytosterols are found in plant foods and are structurally and functionally analogous to cholesterol in vertebrate animals. a-sitosterol, campesterol and stigmasterol are the most commonly occurring phytosterols and constitute 95% of total phytosterols in the diet.

    1.    Wohler (1828) synthesized the first organic compound, urea from inorganic components.

    2.    Emil Fischer (1894) demonstrated the specificity of enzymes and the lock and key relationship between enzyme and substrate.

    3.    Michaelis and Menten (1913) developed kinetic theory of enzyme action

    4.    Watson and Crick (1953) Proposed the double-helical model for DNA structure

    5.    Ingo potrykus (1999) Golden rice- rich in b-carotene.

    6.    Glyoxysomes : Glyoxysomes are temporary in that they occur during transient periods in the life cycle of a plant such as in certain beans and nuts which store fats in their seeds as energy reserves. Glyoxysomes appear in the first few days after seed germination in endosperm cells and associate closely with lipid bodies. They disappear after the storage fats are broken down and converted into carbohydrate.

    1.    Control points are the points in the food processing chain where it is possible to control or remove hazards.

    2.    Critical control points are the control points in the processing chain where it is essential to a hazard, usually because there is no later step at which to establish control.

    3.    Preventative measures are the actions and activities needed to remove hazards or control them by reducing them to acceptable levels.

    4.    Critical limits for critical control points are measurements such as temperature and time, that must be met, or characteristics such as appearance and texture. Critical limits need to be validated.

    5.    Monitoring is the regular measurement or observation of a critical control point to ensure it is not beyond its critical limits.

    6.    Take corrective action to bring the process back under control before the problem (‘deviation’) leads to a safety hazard. Consider proper management (disposition) of any adversely affected product.

    7.    Verification procedures are tests and programmes that make sure the HACCP system working properly

    8.    Record keeping and documentation systems must meet the needs of the business and be adequate to show that the food safety programme is working.

Food Safety and Standards Rules and Regulations-2009

    1.    The Food Safety and Standards Act, 2006 has been passed by the Parliament with the intention to integrate all present food laws into one and to have a single regulatory body.

    2.    Accordingly the Food Safety and Standards Authority of India (FSSAI) has been established under the Food Safety and Standards Act, 2006 (FSS Act, 2006) with the mandate to lay down science based standards for articles of food and to regulate their manufacture, storage, distribution, sale and import, to ensure availability of safe and wholesome food for human consumption.

    3.    In exercise of the powers conferred by section 91 of the Food Safety and Standards Act, 2006 (34 of 2006), the Central Government hereby makes the rules and regulations.

    4.    ‘‘Anti-oxidant’’ means a substance which when added to food retards or prevents oxidative deterioration of food and does not include sugar, cereal, oils, flours, herbs and spices.

1. Food Labelling

Food labelling is the primary means of communication between the producer and seller of food on one hand, and the purchaser and consumer on the other.

Basic Terminology in Food labelling

‘‘Food” means any substance, whether processed, semi-processed or raw, which is intended for human consumption, and includes drinks, chewing gum and any substance which has been used in the manufacture, preparation or treatment of “food” but does not include cosmetics or tobacco or substances used only as drugs.

‘‘Ingredient” means any substance, including a food additive, used in the manufacture or preparation of a food and present in the final product although possibly in a modified form.

Hazard Analysis and Critical Control Point (HACCP) System

1. Identify hazards
 
 It is important to be able to identify the possible microbiological, chemical and physical hazards that can occur at every stage of the food business – from growth, processing, manufacture, storage and distribution, until the point where it is sold to the customer and eaten.

    ·    Microbiological Hazards : Any bacterium, virus, or protozoan that is capable of causing illness and that grows or may be carried on food. Well-known examples of bacteria are Campylobacter, Listeria and Salmonella. The most likely food-borne viruses are the Norwalk type viruses. Giardia is an example of a protozoan that may be food borne. It is important to have some understanding of the risks associated with different types of microbiological hazards.

    ·    Chemical Hazards : Examples include excessive or toxic amounts of heavy metals, chemicals, pesticides, herbicides, insecticides, vitamins, minerals, preservatives, disinfectants, detergents and cleaning compounds. Some hazards may be naturally present such as in green potatoes or taro and rhubarb leaves.

    ·    Physical Hazards : Objects that get into food, or are already present in food, may cause illness, injury or distress to the person eating it. Some examples are glass, metal fragments etc. Other contaminants such as hair or insects may be offensive but not necessarily a danger to health. They should nonetheless be considered and controlled

1. The Stability and Shelf-Life of Food

    1.    In the UK, the date coding to be used is determined by the total life of the product: for microbiologically highly perishable foods, a ‘use by’ date is needed, while for other foods, including foods with more than 18 months’ shelf-life, a ‘best before’ or a ‘best before end’ date is needed.

    2.    IFT (1974) definition of shelf-life, is : The period between manufacture and retail purchase of a food product during which the product is of satisfactory quality.

    3.    Shelf-life is defined as the time during which the food product will :

        i.    remain safe;
        ii.    be certain to retain desired sensory, chemical, physical and 
    microbiological characteristics;
        iii.    comply with any label declaration of nutritional data,

    1.    Water activity (a_w) affects food chemistry and can be controlled by removal (dehydration or drying) or by chemically binding the water, reducing its activity. Water activity in foods ranges from 0.95 to 1.00 in fresh meats and vegetables to 0.20 in dried milk.

    2.    Water activity (a_w) may be defined in a number of ways, qualitatively, aw is a measure of unbound, free water in a system available to support biological and chemical reactions.

    3.    Water activity, not absolute water content, is what bacteria, enzymes, and chemical reactants encounter and is affected by at the micro environmental level in food materials.

    4.    Two foods with the same water content can have very different aw values depending upon the degree to which the water is free or otherwise bound to food constituents.

    5.    The term “water activity” is related to relative humidity. Relative humidity is defined as the ratio of the partial pressure of water vapour in the air to the vapour pressure of pure water at the same temperature.

    6.    Water activity or aw is a property of solutions and is the ratio of vapour pressure of the solution compared with the vapour pressure of pure water at the same temperature. Under equilibrium conditions water activity equals:

Food and Industrial Microbiology

    1.    Microbiology often has been defined as the study of organisms and agents too small to be seen clearly by the unaided eye—that is, the study of microorganisms

    2.    Two bacteria that are visible without a microscope, Thiomargarita and Epulopiscium.

    3.    Procaryotic cells [Greek pro, before, and karyon, nut or kernel; organism with a primordial nucleus] have a much simpler morphology than eucaryotic cells and lack a true membrane-delimited nucleus. All bacteria are procaryotic.

    4.    Eukaryotic cells [Greek eu, true, and karyon, nut or kernel] have a membrane- enclosed nucleus; they are more complex morphologically and are usually larger than procaryotes. Algae, fungi, protozoa, higher plants, and animals are eucaryotic.

    5.    The ordinary microscope is called a bright-field microscope because it forms a dark image against a brighter background

Food Packaging

    1.    Food packaging : A wrap, pouch, bag, box, cup, tray, can, tube, bottle and jar are some of the many forms of packaging that contain food products. It must contain a food product as well as protect and preserve it for a specific length of time. Each package must, by law, display product information for the consumer.

    2.    Low Density Polyethylene (LDPE) is an economical material with low WVTR; however, it has high permeabilities to flavours/volatiles, poor grease resistance and are limp.
 
    3.    High-Density Polyethylene (HDPE) is stiffer, more translucent and has better barrier properties but needs higher temperature for sealing.

    4.    A copolymer of polyethylene and poly vinyl alcohol, and EVOH has outstanding gas barrier properties especially when dry.

    5.    High-density polyethylene is stiff, strong, tough, resistant to chemicals and moisture, permeable to gas, easy to process, and easy to form. It is used to make bottles for milk, juice, and water; cereal box liners; margarine tubs; and grocery, trash, and retail bags.

    6.    Low-density polyethylene is flexible, strong, tough, easy to seal, and resistant to moisture. Because low-density polyethylene is relatively transparent, it is predominately used in film applications and in applications where heat sealing is necessary. Bread and frozen food bags, flexible lids, and squeezable food bottles are examples of low-density polyethylene.

Instructions for establishment registration and processing, filing for acidified and low-acid canned foods

Acid Food
 
A food that has a natural pH of 4.6 or below.
 
Acidified Food
 
A low-acid food to which acid(s) or acid food(s) are added and which has a finished equilibrium pH of 4.6 or below and a water activity (aw) greater than 0.85.
 
Authorized Company Representative
 
The person authorized by the company to sign the registration and process filing forms on its behalf. That person should possess the knowledge necessary to answer technical questions concerning filed processes.

1. Fundamental Concepts of Unit Operations

    1.    Process is the set of activities or industrial operations that modify the properties of raw materials with the purpose of obtaining products to satisfy the needs of a society.

    2.    Food process engineering includes the part of human activity in which the knowledge of physical, natural, and economic sciences is applied to agricultural products as related to their composition, energetic content, or physical state.

    3.    Food process engineering can also be defined as “the science of conceiving, calculating, designing, building, and running the facilities where the transformation processes of agricultural products, at the industrial level and as economically as possible, are carried out.

    4.    In a discontinuous operation the raw material is loaded in the equipment; after performing the required transformation, the obtained products are unloaded. These operations, also called “batch” or “intermittent,” are carried out in steps:

    1.    Microbial Risk Assessment (MRA) can be considered a tool for use in the management of the risks posed by food borne pathogens and in the elaboration of standards for food in international trade.

    2.    MRA comprises risk assessments of particular pathogen - commodity combinations

    3.    According to codex alimentarious commission (CAC) microbial risk assessment is defined as a scientifically based process consisting of 4 components.

        ·    Hazard identification
        ·    Exposure assessment
        ·    Hazard characterization
        ·    Risk characterization

Storage Systems

    1.    Low cost storage technology

        ·    In situ : Means delaying the harvest until the crop is required and employed 
    for the root crops.
        ·    Sand and Coir : Used for the Potatoes
        ·    Pits used for the Potatoes
        ·    Clamps used for the Potatoes
             Wind breaks used for the Onions – 6 months 1m height wooden stakes
        ·    Cellars : under ground / partly under ground. Ex: Apples, cabbage, 
    onions and potatoes during winter.
        ·    Barns : In Sudan onions are stored in palm leaf barns called “Rakubas”.
        ·    Evaporative Coolers
        ·    Zero energy cooling chambers
        ·    Night ventilation.

    2.    As a general rule, air circulation of storage room is designed to provide about 25m² / min / 3000 K cal of refrigeration predicated on a 5.5ºC

    3.    Frozen storage refers to storage at temperature where food is maintained in frozen conditions.

    4.    Good frozen storage generally means - 18ºC / below.

    5.    Below – 9.4ºC, there is no significant growth of microorganisms in food.

    1.    Food : defined as something which is eaten / drunk by human beings or something we eat and which can be utilized by our body.

    2.    Milk and milk products like Dahi, yoghurt, paneer, cheese, Khoa etc these foods are rich sources of Vitamin A, Riboflavin and Calcium.

    3.    Meat, fish, poultry etc besides proteins these foods also provide Iron and B- complex Vitamins.

    4.    Eggs and egg products are also rich in Iron, Vitamin A and Riboflavin (B1).

    5.    Pulses, nuts and oil seeds are also a good source of thiamin, niacin, and Iron.

    6.    Strategies and measures for alleviating the world food problem require.

Food Processing Technology

    1.    The transition from solid to fluid and back is known as a phase transition and this is important in many types of food processing (e.g. water to water vapour in evaporation and distillation and dehydration; water to ice in freezing and freeze drying or freeze concentration or crystallisation of fats.
 
    2.    Phase transition takes place isothermally at the phase transition temperature by release or absorption of latent heat, and can be represented by a phase diagram.

    3.    A second type of transition, known as glass transition, takes place without the release or absorption of latent heat and involves the transition of a food to an amorphous glass state at its glass transition temperature.

    4.    The transition is dependent on the temperature of the food, time, and the moisture content of the food.

    5.    The density of a material is equal to its mass divided by its volume and has units of kgm^-3

    1.    Vegetables are less acidic than fruits and food poisoning bacteria are able to grow in many vegetable products

    2.    Different levels of acidity in fruits

    3.    Water for cleaning of fruits and vegetables should contain about 200 ppm of chlorine (by mixing 1 litre of bleach into 250 litres of water)

    4.    Water that is used as an ingredient in fruits and vegetable product should not contain more than 0.5 ppm chlorine (by adding 2.5 ml of bleach to 250 litres of water), to avoid contaminating products with a chlorine smell

1. Herbs and Spices
       
    1.    International Standards Organization (ISO) defines spices and condiments as: Vegetable products or mixtures thereof, free from extraneous matter, used for flavouring, seasoning and imparting aroma in foods.

    2.    Rosengarten describes a spice as a product which enriches or alters the quality of a thing, for example altering the taste of a food to give it zest or pungency; a piquant or lasting flavouring; or a relish. The term ‘spice’ is thus used to cover the use of spices, herbs and certain aromatic vegetables to impart odour and flavour to foods.

    3.    Herbs may be defined as the dried leaves of aromatic plants used to impart flavour and odour to foods with, sometimes, the addition of colour. The leaves are commonly traded separately from the plant stems and leaf stalks.

Nutraceuticals and Health Foods

    1.    According to the International Food Information Council (IFIC), functional foods are “foods or dietary components that may provide a health benefit beyond basic nutrition.”

    2.    Nutraceuticals : A product isolated and purified from foods that is generally- sold in medicinal forms are usually associated with food. A nutraceutical is demonstrated to have a physiological benefit or provide protection against chronic disease (Coined originally by Stephen DeFelice in 1989, founder and chairman of the Foundation for Innovation in Medicine, USA).

    3.    The International Life Sciences Institute of North America (ILSI) has defined functional foods as “foods that by virtue of physiologically active food components provide health benefits beyond basic nutrition

    4.    Health Canada defines functional foods as “similar in appearance to a conventional food, consumed as part of the usual diet, with demonstrated physiological benefits, and/or to reduce the risk of chronic disease beyond basic nutritional functions.”

    1.    The heifer is bred (naturally or by insemination) before the age of two years.

    2.    The gestation period is nine months and one week.

    3.    After calving, the cow gives milk for 10 months.

    4.    1 – 2 months after calving the cow will again be bred.

    5.    The cow then continues to give milk for about 300 days. This period is known as lactation.

    6.    Milk should preferably be handled in a closed system, to minimise the risk of contamination. It must be cooled to 4 °C as soon as it is produced.

    7.    The content of protein, lactose and ash is somewhat higher in buffalo milk than in cow milk. Buffalo milk contains vitamin A, but lacks b-carotene, which is present in cow’s milk.

    8.    When milk and cream turn to butter, there is a phase inversion from an oil-in-water emulsion to a water-in-oil emulsion.

    9.    In milk, the whey proteins are in colloidal solution and the casein in colloidal suspension.

Meat and Meat Products

    1.    Meat can be defined as “the muscle tissue of slaughter animals”.
    2.    meat is composed of water, fat, protein, minerals and a small proportion of carbohydrate

Cereal Processing

    1.    The protein content of wheat grain varies widely, but for bread making a value of at least 11% is required.

    2.    Low grain sulphur will result in low concentrations of the sulphur-containing amino acids, cystine and methionine, and may result in poor loaf volume.

    3.    During seed germination endosperm starch is converted into soluble glucose and maltose to support the developing embryo. This is brought about by enzymatic activity, especially the enzyme a-amylase.

    4.    Excessive a-amylase levels result in the formation of a darkened loaf crust as a result of sugar caramelization and a sticky crumb structure which can cause problems during slicing.

    5.    Wheat with a protein level below 10% is preferred to reduce gluten elasticity.

    6.    There is a very good correlation between grain nitrogen and the amount of malt extract achieved, low grain nitrogen giving more fermentable extract

    7.    The term ‘coarse grains milling’ is a very broad one that refers to the comminution of the berries of the wheat, barley and other coarse grain crops.

Moisture Content
 
Two maximum moisture levels are provided to meet different climatic conditions and marketing practices. Lower values in the first column are suggested for countries with tropical climates or when long-term (more than one crop year) storage is a normal commercial practice. The values in the second column are suggested for more moderate climates or when other short term storage is the normal commercial practice.

Introduction

    1.    Food Science is the application of the physical, biological and behavioral science to the processing and marketing of foods (Margaret, 1968).

    2.    Technology can be defined as the science dealing with knowledge of doing things efficiently and effectively.

    3.    Food science helps us understand the theory example: what methods can best be used to store and preserve food to maintain quality and prevent spoilage.

    4.    Food technology demonstrates how the food is stored, preserved, processed and transported and has been responsible for development of new techniques for processing and preservation of food on a commercial scale and for packaging it in such a way that it can be sold conveniently.

    5.    Food science deals with the study of facts of the physical, chemical and biological sciences as they influence the processing and preservation of food.

    6.    Food technology deals with engineering and other scientific and technical problems involved in transforming edible raw materials and other ingredients into safe, nutritious and appetizing food products (Desrosier, 1977).

    7.    Food science is concerned with basic scientific facts about food, where as food technology concerned with the processing of raw materials into foods that meet human needs and wants.

    8.    Indian agriculture has undergone a sea change during the past half a century. Starting with the campaign for “grow more foods” in the forties, India reached self sufficiency during the eighties.

Instrumentation and Advanced Analytical Techniques

    1.    Chromatography is defined as an analytical technique where by a mixture of chemicals may be separated by virtue of their differential affinities for two immiscible phases. One of these, the stationary phase, consists of a fixed bed of small particles with a large surface area, while the other, the mobile phase or “eluant”, is a fluid that moves constantly through, or over the surface of, the fixed phase.

    2.    The chromatogram evaluated qualitatively by determining the Rf or retention factor, for each of the eluted substances.

    3.    The Rf is a measure of that fraction of its total elution time that any compound spends in the mobile phase.

    4.    If the liquid phase is a polar substance (e.g. polyethylene glycol) and the mobile phase is non polar, the process is termed normal-phase chromatography

    5.    When the stationary phase is non polar (e.g. octadecylsilane) and the mobile phase is polar, the process is reversed-phase chromatography

    6.    The chromatographic separation of mixtures of large molecules such as proteins may be accomplished by a mechanism called size exclusion chromatography or gel permeation chromatography.

    7.    Separation of certain molecules is accomplished by a mechanism called affinity Chromatography in which specific binding between an antibody (stationary phase) and antigen (analyte) occur.

1. Guidelines on Analytical Terminology

    1.    Accuracy : The closeness of agreement between a test result or measurement result and a reference value.

    2.    Analyte : The chemical substance sought or determined in a sample. This definition does not apply to molecular biological analytical methods.

    3.    Applicability : The analytes, matrices, and concentrations for which a method of analysis may be used satisfactorily.

    4.    Bias : The difference between the expectation of the test result or measurement result and the true value. In practice conventional quantity value can be substituted for true value.

    5.    The bias of a measuring instrument is normally estimated by averaging the error of indication over the appropriate number of repeated measurements. The error of indication is the: “indication of a measuring instrument minus a true value of the corresponding input quantity”.

    6.    Calibration : Operation that, under specified conditions, in a first step, establishes a relation between the values with measurement uncertainties provided by measurement standards and corresponding indications with associated measurement uncertainties and in a second step uses this information to establish a relation for obtaining a measurement result from an indication.

    7.    Certified reference material (CRM) : Reference material accompanied by documentation issued by an authoritative body and providing one or more specified property values with associated uncertainties and traceability, using valid procedures.

Physical Chemistry of Foods

    1.    Thermodynamics is primarily concerned with energy and entropy. Energy, also called internal energy (U), comprises heat and work; it is measured in joules (J).

    2.    Energy may be recalled that work generally equals force times distance (in N_·m = J) and that force equals mass times acceleration (in kg ·m· s^-2 = N).

    3.    According to the first law of thermodynamics, the quantity of energy, i.e., heat (q) work (p) potential energy, is always preserved.

    4.    Entropy (S)  is a measure of disorder; it is given by   
        S = K_B ln Ω
        Where K_B is the Boltzmann constant (1.38 ×10^-23 J × K^-1) and W is the number of  ways in which the system can be arranged, also called the number of degrees of freedom.

    5.    Colligative solution properties : The lowering of the chemical potential of a solvent by the presence of a solute causes changes in a number of physical properties: vapor pressure, boiling point, freezing point, osmotic pressure, etc. (the magnitudes of these changes all are proportional to the mole fraction of solute).

Percent Solutions

There are three types of percent solutions. All are parts of solute per 100 total parts of solution. Based on the following definitions you may calculate the concentration of a solution or calculate how to make up a specific concentration

    1.    % W/W - Percent of weight of solute in the total weight of the solution. Percent here is the number of grams of solute in 100 grams of solution. 
        Example: 
        A 100% (W/W) NaCl solution is made by weighing 100 g NaCl and dissolving in 100 g of solution

    2.    % W/V - Percent of weight of solution in the total volume of solution. Percent here is the number of grams of solute in 100 ml of solution. This is probably the least significant way of naming a solution, but the most common way of doing it. In fact, any percent solution not stipulated as W/W, W/V, or V/V is assumed to be % W/V. 
        Example : A 4% (W/V) NaCl solution is 4 g of NaCl in 100 ml of solution.

    3.    % V/V - Percent of volume of solute in the total volume of solution %V/V. Percent here is the number of milliliters of solute in 100 ml of solution. 
        Example: A 10% (V/V) ethanol solution is 10 ml of ethanol in 100 ml of solution; unless otherwise stated, water is the solvent. 

    1.    Absorbance (A) - Defined as –log (1-a) = log (1/t), where ‘a’ is the absorbance and‘t’ the transmittance of a medium through which a light beam passes.

    2.    Absorption coefficient (a) - The relative decrease in the intensity of a collimated beam of electromagnetic radiation, as a result of absorption by a medium, during traversal of an infinitesimal layer of the medium, divided by the length traversed.

    3.    Acceleration due to gravity (g) - The standard value (9.80665 m/s2) of the acceleration experienced by a body in the earth’s gravitational field.

    4.    Acid - Historically, a substance that yields an H+ ion when it dissociates in solution, resulting in a pH< 7. In the Brönsted definition, an acid is a substance that donates a proton in any type of reaction. The most general definition, due to G.N. Lewis, classifies any chemical species capable of accepting an electron pair as an acid.

    5.    Acid dissociation constant (Ka) - The equilibrium constant for the dissociation of an acid HA through the reaction HA + H2O1 A- + H3O+. The quantity pKa = -log Ka is often used to express the acid dissociation constant

    6.    Acyl groups - Groups formed by removing the hydroxy groups from oxoacids that have the general structure RC (=O) (OH) and replacement analogues of such acyl groups.

    7.    Adiabatic process - A thermodynamic process in which no heat enters or leaves the system.

    1.    Nutrients : Chemical substances obtained from food and used in the body to provide energy, structural materials, and regulating agents to support growth, maintenance, and repair of the body’s tissues. Nutrients may also reduce the risks of some diseases.
    2.    Carbohydrate, fat, and protein are sometimes called macronutrients because the body requires them in relatively large amounts (many grams daily). In contrast, vitamins and minerals are micronutrients, required only in small amounts (milligrams or micrograms daily).
    3.    The international unit for measuring food energy is the joule, a measure of work energy. To convert kcalories to kilojoules, multiply by 4.2; to convert kilojoules to kcalories, multiply by 0.24.
    4.    Essential nutrients : Nutrients a person must obtain from food because the body cannot make them for itself in sufficient quantity to meet physiological needs; also called indispensable nutrients. About 40 nutrients are currently known to be essential for human beings. Energy-yielding nutrients: the nutrients that break down to yield energy the body can use:

In most countries there is no legislative definition of the term drawing a border line between conventional and functional foods is challenging even for nutrition and food experts. Functional foods do not have a specific place in food law; consequently there is a great deal of confusion about what they are, could or should be. A common perception is that functional foods are foodstuffs either with one or more specific added ingredients (components), or certain substances of common ingredients (components) removed and which are claimed to have a positive influence on human health or well being. The term ‘functional foods’ for this group of products is considered to be an unfortunate one since all foodstuffs are or should be functional and a more appropriate term might be helpful.

Introduction
 
The future of functional foods is bright and there are several reasons for the growing interest in “functional” foods, such as awareness of personal health deterioration, led by busy lifestyles with poor choices of convenience foods and insufficient exercise; increased incidence of self-medication; increased level of information from health authorities and media on nutrition and the link between diet and health; scientific developments in nutrition research; and a crowded and competitive food market, characterized by pressurized margins. Now, the consumer is well aware of the relationship between diet and health. Consumers understand that the risk for chronic diseases such as heart disease, stroke, colon cancer, and type II diabetes is possibly preventable by modification in their diet pattern. This scientific progress in understanding the relationship of diet to disease, along with increasing health care costs and the consumer’s desire to initiate healthy eating and lifestyle habits, makes this a potentially fertile ground for the development of food products that meet these demands.

Introduction

The search for weight controlling foods began in the mid 19^th century when industrialization led to widespread obesity. Over the years, people have tried to lose weight by avoiding particular food groups like carbohydrates, eating specific foods such as cabbage soup, or adding a certain ingredient, like vinegar or grapefruit to each meal. Recently scientists have demonstrated that green tea, dairy products, and cinnamon have the potential to improve weight control by altering fat and/or carbohydrate metabolism at the sub-cellular level. Considerable research is needed, however, before these food items can be used to develop effective weight management products.

Introduction
 
Diabetes shows a wide range of variation in prevalence around the world and it is expected to affect 300 million by the year 2025. In a prevention framework where banning policies and educational strategies lead the interventions, functional foods (FFs) with their specific health effects could, in the future, indicate a new mode of thinking about the relationships between food and health in everyday life. Functional ingredients, such as stevioside, cinnamon, bitter melon, garlic and onion, ginseng, Gymnema sylvestre and fenugreek, have been addressed for their specific actions towards different reactions involved in diabetes development. New strategies involving the use of FF should be validated through large scale population trials, considering validated surrogate end points to evaluate the effect of FF in prevention of chronic diseases such as type 2 diabetes mellitus.

Introduction
 
Cardiovascular disease (CVD) is still a major cause of death in Western populations and is becoming an important cause of morbidity and mortality worldwide. Thanks to advanced medical knowledge and treatments, many patients survive an initial event. Because of that, prevention of secondary CVD is a growing task for nutritionists and other health professionals. Cardiovascular risk can be reduced by lifestyle changes, one of which is diet. There is now substantial evidence from epidemiological and clinical studies that a diet rich in fruits, vegetables, unrefined grains, fish and low-fat dairy products, and low in saturated fats and sodium, can reduce the risk of coronary heart disease and hypertension.

Introduction
 
Developments in innovative food packaging technologies have evolved in response to the need for protection of the food product containing bioactive component from both external and internal environments and in response to consumer expectations for health claims, convenience and product safety. Consumers around the world are willing to pay extra for guaranteed product freshness delivered by the type of packaging. Many new packaging developments have focused on extending the shelf life of the product and on delivering a higher quality product to the consumer. These developments would not be possible without significant advances in the materials used in packaging and the incorporation of various types of sensors into food packaging. This chapter outlines the innovative packaing solutions in the production of functional food.

Introduction
 
The emerging functional foods market presents a considerable challenge to all manufacturers to keep abreast of the use of adequate packaging materials for their products. The interest in functional foods is intrinsically linked to the potential health benefits of their bioactive components. Preserving these bioactive components is, therefore, a crucial factor to providing the anticipated health benefits of functional foods. However, recurring failure in maintaining the quality of functional foods has been reported. Functional foods include a wide variety of products such as prebiotics and probiotics, dried foods (fibers, probiotics, tea, herbs, etc.), fermented products (yogurts, kefirs, vegetables), and fresh fruits and vegetables.