eChapters

10.1 Introduction
Developed in the 1950s, ultra-high temperature pasteurization typically involves no hold using a temperature range of 135°C to 150°C. Immediate aseptic packaging determines how well UHT heat treatment of milk works.
10.2 Uperization Developed in Switzerland, this is generally known as “ultra – pasteurization”. Milk is heated in this method under direct steam up to 150 °c for a fraction of a second. One is always learning from this. Fore warming milk to 50 °C is the first stage in the uperization process, meant to eliminate most of the dissolved oxygen and volatile off-flavours by vacuum treatment. In the second step, the milk is first preheated to roughly 80–90 °C and then heated on the uperization chamber under high-pressure steam to roughly 150 °C for ½ to ¾ a second. The product moves into an expansion chamber at almost atmospheric pressure after this heating, therefore inducing some evaporation of moisture. The good is then transferred to a chiller and subsequently into a storage chamber.
The advantages of uperization are
• Long keeping quality.
• Removal of feed and other volatile off-flavours:
• Appreciable homogenization effect.
• Reduction in acidity.
• Efficient destruction of microorganisms.
• Effect of uperization on nutritive value and flavour are not greater than that of pasteurization.

Introduction

Organic substances consumed for nutritional purposes can be called as “food”. Food contains biomolecules, like, protein, lipid and carbohydrate, inorganic minerals as salts, and organic substances, such as, vitamins, emulsifiers, acids, antioxidants, pigments, polyphenols and flavour-producing compounds. They can be of plant or animal origin. Depending on the durability in terms of suitability of consumption, foods can be perishable, semi-perishable and non-perishable. Food spoilage occurs due to chemical, microbial, or physical actions. eventually affecting its nutritional values, colour, edibility and texture (M. Rahman, 2007).

The cold chain is the lifeline of the ice cream industry because the product must remain continuously frozen from factory hardening until consumption. This chapter examines the complete cold-chain network, beginning with hardening and factory cold storage and extending through CFA or distributor cold rooms, refrigerated transport, retail freezers, and final consumer handling. It explains temperature requirements, monitoring systems, equipment selection, cold-room management, stock rotation, and quality assurance. Particular attention is given to Indian challenges such as power interruptions, inadequate freezer maintenance, poor retailer practices, and temperature abuse. The chapter demonstrates how disciplined cold-chain management protects texture, flavour, shape, overrun, shelf life, and ultimately consumer confidence

INTRODUCTION

Food processing is necessary for the conversion of raw food products in to finished products with desired textural, sensory and nutritional attributes. This is required to be done mainly due to seasonal production and facilitating non-seasonal availability of different commodities. Stages of processing are primary and secondary. In primary processing raw material is converted into food commodities, while in secondary processing commodities are converted into edible products. Processing is mainly done to convert the products into edible forms, to extend shelf life, availability, accessibility and also to provide variety into the form of value added products. Processing of products destroys inhibitors, microorganisms, retards physico-chemical and biochemical deterioration, maintains good sensory properties, preserves and enhances the nutritive value. Unit operations involve phase changes and sometimes chemical reactions also, which are undesirable as they cause inferior quality of the product (Gekas 1992). Different processes like separation, assembly, preservation and packaging are broader classification for unit operations in food processing (Saravacos and Kostaropoulos 2002).

1. Fundamental Concepts of Unit Operations

    1.    Process is the set of activities or industrial operations that modify the properties of raw materials with the purpose of obtaining products to satisfy the needs of a society.

    2.    Food process engineering includes the part of human activity in which the knowledge of physical, natural, and economic sciences is applied to agricultural products as related to their composition, energetic content, or physical state.

    3.    Food process engineering can also be defined as “the science of conceiving, calculating, designing, building, and running the facilities where the transformation processes of agricultural products, at the industrial level and as economically as possible, are carried out.

    4.    In a discontinuous operation the raw material is loaded in the equipment; after performing the required transformation, the obtained products are unloaded. These operations, also called “batch” or “intermittent,” are carried out in steps:

Different basic as well as advanced laboratory equipment need to be used in a laboratory while conducting experiments. Many of them like pH meter, laminar air-flow hood, viscometer, thermometer, magnetic stirrer, microwave ovens, incubators, sand-bath, water-bath, oil-bath, etc. are the basic and simple equipment which require to be used routinely while conducting experiments in a laboratory. However, basic knowledge of their operation, handling procedure, when to be used, how to be used, and more importantly what not to be done with it, etc. are essential for keeping them in serviceable condition as well as for the accuracy/precision in their operation. In this chapter, we will discuss about some of the routinely used laboratory equipment along, while some other sophisticated equipment will be discussed in other chapters.

pH Meter

A pH meter measures exactly the H+ concentration of a solution; thus indicates acidity or alkalinity of a solution with greater precision. Actually, it measures the difference in electrical potential between a pH electrode and a reference electrode, and display the results converted into the corresponding pH value. Electrode is the key part (rod-like structure, usually made of glass) of a pH meter, having a bulb at the bottom containing a sensor. The pH meters range from simple and inexpensive pen-like devices (portable/pocket pH meter) to complex and expensive laboratory instruments with computer interface (Figure 5.1).

Frequent calibration of a pH meter using the solutions of known pH (standard solution of pH 4.01, 7.00, 10.01, Figure 5.2), generally before each use, ensures the accuracy of the instrument. For very precise work the pH meter should be calibrated before each measurement. For normal use calibration should be performed at the beginning of each day. This is necessary because the glass electrode does not give a reproducible measurement over a longer period of time.

Introduction

To accomplish experimentations in laboratories, sometimes we need microscope to test/validate our ideas/findings. A microscope is needed to examine objects which are very small which cannot be visualised by naked eyes. However, microscopic study requires some expertise in operating the microscope, particularly the sophisticated microscopes, as well as to prepare (peeling, sections, cutting, fixation, staining, dehydration, mounting, etc.) the sample, so that it can be appropriately examined for the anticipated details.

According to the scientific requirements different types of microscopes are used in the laboratories for research purpose. In this chapter, our aim is to acquaint you with the basics of using and handling of microscopes, particularly a compound microscope that is commonly used by the graduate and post graduate students for basic research.

Microscopy is often designated as the scientific investigation that is employed to review and analyse minute structures and objects using a microscope. The term “microscope” has been derived from ancient Greek words “micros” meaning “small” and “skopeîn” meaning “to look at or see,”.

Microscope

A microscope is a laboratory instrument used to examine objects (biological specimens) which are too small to be seen by the naked eye. The microscope must accomplish three tasks:

• Magnification- to provide a magnified image of the specimen.

• Resolution- to separate the details in the image.

• Contrast- to render the details clearly visible to the eyes.

Although we will tnroduce to you the different types of microscopes used for different speciments, our main focus is to discuss about compound microscope, which is commonly used in the laboratories. Compound microscope was developed around the beginning of 17th century.

A variety of glassware like burettes, pipettes, measuring cylinders, flasks, separating funnel etc. are used for experimental purposes in a laboratory. All of these need to be used and handled correctly, otherwise the chances of experimental error and damage to the glassware increase significantly. Being made of glass, they need special care while washing and using in experiments because even a minor mistake in handling of glassware may result in breakage of the apparatus. With this precautionary note, let us discuss about some of the specific glassware used in the laboratories.

Burette

Burette is a long graduated glass tube with a stopcock at one end for delivering a known/desired volume of a liquid, especially in titration experiments. It contains a stopcock at the lower end and a tapered capillary tube as the stopcock’s outlet. The flow rate of liquid from the tub stopcock’s outlet can be controlled with the help of stopcock valve. Burette is used for measuring the volume of a solution/chemical added in a quantitative (titrimetric) estimation experiment.

Procedure of using a Burette

The burette reading must be noted before and after delivering the chemical/ solution from the burette. The difference in these two readings is the volume of the chemical/solution released out of the burette. The solution should be released slowly/drop-by-drop to avoid any experimental error/mistake. If the solution/chemical is allowed to pour out too fast, the wall of the burette will not drain properly and some liquid may remain sticking to the surface of the walls. Moreover, excessive amount of chemical may be released/dropped during the experiment, particularly during the titration experiment wherein we need to know the exact amount of acid/base needed to get neutralization point. Thus, any minor mistake may lead to get a faulty reading. The measuring capacity of a burette usually used in a laboratory is 50 mL.

Since mouth pipetting of liquid reagents in a laboratory is not advisable, different tools are needed for safe, easier, and precise dispensing of liquid reagents. This chapter is devoted to make you aware of the tools like vacupette and micropipette which can be used for the handling of liquid reagents in a safe and precise way.

Vacupette

Glass pipettes have been traditionally used by sucking liquid solvent/solution directly using mouth. For safety reasons, mouth pipetting is no longer permitted in a laboratory. Hence, to fill a pipette vacupette is used by placing it on the top of glass or plastic pipette. It acts as a device to create vacuum for filling reagent in the pipette as well as for releasing the reagent with mechanical regulation on the flow out of the liquid. Vacupette can be of different types.

1. Rubber Bulb Vacupette

Rubber bulb vacupette is often used to transfer (pump up and discharge) liquid using a pipette when a larger quantity of chemicals is to be transferred. Small/tiny rubber bulb is attached to glass/plastic pipette for drawing little amount of reagents. Small vacupette is used to precisely discharge smaller volume/ drops.

Handling of Vacupette

• Insert the top of a pipette in the bottom of the rubber bulb vacupette (Figure 6.1).

• Remove air from the pipette by squeezing valve “A” at the top of the vacupette while simultaneously squeezing the rubber bulb. The amount of air released depends on the size of the rubber bulb.

Introduction

Food ingredients sector is indicating ample prospects for growth. The segment is an obligatory element for the growth of the food and beverage sector. These ingredients are also driving the development of the healthy functional foods industry to increase by 20% growth annually. Innovation in the food and beverage market, especially in the processed food domain, has created innumerable opportunities for new product development in the ingredients market as well. The segment is driven by the growing food processing industry and India is emerging as ingredients bowl of the future. Additionally, growing urbanization coupled with industrialization and changing lifestyles have led to growing demand for convenient food that offers health benefits, over and above the basic nutritional benefits of the foodstuff. There are nearly 10 to 15 broad classes of ingredients that include the very basic ingredients as well as certain value added ingredients that have niche applications. Key ingredients are preservatives, flavours, colours, hydrocolloids/stabilizers, emulsifiers, artificial sweeteners and value added health food ingredients such as omega-3-fatty acids prebiotics, probiotics etc. Until recently, there has been limited research into functional foods in meats, while functional foods in the dairy sector have been developed to a high degree. But meat is less allergenic than many other foods and much more research into the development and marketing of novel functional meat products is required. Such meat products would open up a new market for the meat industry. Nutraceutical and functional food industry is a very vibrant industry today with the claims and a clear mandate of health, wellness and prevention of diseases as one of its main goals.

Introduction

It became clear from the discussions of previous chapters that probiotics perform metabolic functions such as fermenting indigestible dietary residues and endogenous mucus, production of vitamins, and absorption of minerals. They also play key role in intestinal epithelial cell proliferation and differentiation, and the homeostasis of the immune system. Further, probiotics are also known to involve in modifying gut pH, antagonizing pathogens through the production of antimicrobial compounds, competing with pathogens for recept or sites as well as for available nutrients and growth factors, stimulating immunomodulatory cells of the host, and produce a wide range of enzymes such as lactase, amylase, lipase, protease, phytase and so on. The molecular mechanisms of probiotics are largely unknown and opens avenue for research in probiotics. Moreover, current research in probiotics and health consciousness in the public lead to an increased interest towards probiotics from the public, researchers, governmental organizations (such as the WHO/FAO) and medicine and food companies. In this chapter, application of probiotics in treating various diseases and disorders are discussed.

Introduction

COVID-19, a worldwide pandemic, addresses a deflationary stun for the worldwide economy. The outbreak of coronavirus has had a significant impact on public health worldwide and all stages of various industries’ supply chain and value chain. The 2019 novel coronavirus (2019-nCoV) or the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is rapidly spreading from its origin in Wuhan City of Hubei Province of China to the rest of the world. The infection began in bats and was communicated to people by inhalation or contact with infected droplets, and its infection time frame goes from 2 to 14 days. Primary manifestations in hospitalized patients with COVID-19 include fever (98%), cough/hack (76%), and myalgia or weakness (44%). Severe complications include acute respiratory distress syndrome (ARDS), RNAemia, acute cardiac injury, and multiple organ failure. However, exaggerated immune responses may lead to Cytokine Storm. Cytokine storm syndrome includes ARDS, shock, and multi-organ failure (Zheng et al., 2020). Many people are asymptomatic also, and the severity of this disease varies from person to person. The fatality rate is estimated to range from 2 to 3%. Typical/low white cell checks can analyze it with raised C-responsive protein (CRP), periodic modernized tomographic chest examination. The infection spreads quicker than its two predecessors, the SARS-CoV and Middle East respiratory disorder Covid (MERS-CoV); however, it has lower casualty. The worldwide effect of this new scourge is yet questionable (Singhal, T. (2020).

Abstract

The present study was conducted to develop ready to eat extruded snack food based on germinated flour of wheat, Bengal gram and green gram. A Central Composite Rotatable Design (CCRD) for five variables at five levels was used for experimental combinations and optimization of the process parameters. Experimental design with green gram flour (5 to 20%) and bengal gram flour (1535%) in wheat flour, moisture content (14-18%), screw speed (200-300 rpm) and barrel temperature (126-134°C) as independent variables having 32 different combinations of snack formulations that were studied using response surface methodology to investigate the effect of these variables on product responses (Lateral expansion, bulk density, Water absorption index, water solubility index and sensory characteristics). Sensory analysis was carried out of all extrudates for texture, color and overall acceptability. Multiple regression equations were obtained to describe the effects of each variable on product responses. The optimum operating conditions for development of germinated wheat, green gram and Bengal gram flour extrudate were 132°C of barrel temperature, 275 rpm of screw speed, 17 % offeed moisture, 6.5% of green gram flour and 23.1% of Bengal gram flour. The responses predicted by the software for these optimum process conditions resulted lateral expansion of 169.59%, bulk density, 0.263g/cm³; water solubility index, 19.08; water absoption index, 13.88g/g; and overall acceptability value of 7.38.

Introduction

Whey is the serum or watery part of the milk that remains after separation of curd that result from the acid or proteolytic enzyme mediated co-agulation of milk. It is major by product of dairy industries manufacturing products like channa, paneer, cheese and chakka (Sharma and Bhatia, 1999).The current world production of whey is estimated at about 165 million tones, in that cheese whey accounts for nearly 95 % of total whey. In India near about one million tonne of whey is annually derived, in that major source of whey is from the production of channa, paneer and shrikhand production. This is mostly thrown away as a waste which creates environmental pollution, also in that way about 70, 000 tonnes of Nutritious solids are lost (Puranik, 1999).

11.1 Introduction

Fermented foods are the result of the activity of a few specialized types of micro-organisms among the thousands of species known to mankind. Success of such processes is depended upon the control of environmental conditions ensuring the growth of the desired microbial species and inhibition of others. The fermentation can be broadly defined as the process in which the carbohydrate or like compounds are broken down, under anaerobic or aerobic conditions [171]. The basic role of micro-organisms is to breakdown the organic compounds to get energy for their metabolic activities. From ancient times, people all over the world have developed traditional processes for production of alcoholic beverages (wine) either from natural sugar such as in wine or by degrading the starchy materials (beer). Fruit wines referred to as ‘Soma’ in our ancient scriptures have been part of food of man and were used as medicine, might have originated accidentally (transformation of fruit juice beverages having exhilarating or stimulating properties). Wines are made principally from fruits like grape or extract of other fruits of various types (table wine, sparkling wine, dessert, aperitif or vermouth and pop wines) while the beers are prepared from grains like barley, rice and corn. The principal carbohydrate in grain is starch which can not be fermented directly. This process is brought about by starch splitting enzymes by germinating the grains followed by drying and storage until needed. Distillation of beer produces a variety of liquors (whisky, rum, gin, vodka), while that of wine produces the brandy. Besides, the improvement in the raw material selection, fermentation conditions and techniques, a lot of waste material is generated during fermentation process in the industries. Nevertheless, the food based industries are significant contributors to the economy of the many countries of the world (Fig 1). The global market value of food fermentation based industries is estimated to be around 250 million US dollars. Interestingly, more than 10% of this accounts for alcoholic beverages (Fig 2) with projected increase of US$ 27 billions in 1989 to 40 billions in 1990 [93,123,123,129].

Lanolin, commonly known as Wool grease is primarily a lipid secretion from sebaceous glands of wool bearing animals. The role of Lanolin in nature is to protect wool and skin of animal from climate and the environment. It is an excellent emollient, self-emulsifying and dermatological safe for skin applications. Lanolin is a complex matrix since it is a mixture of high molecularweight esters of fatty acids with fatty alcohols, sterols, tri-terpene alcohols; diesters, long-chain alcohols & diols, long-chain fatty acids and hydroxy acids and is therefore difficult for effective matrix clean-up. Because of its water proofing and antimicrobial properties, lanolin is used mainly in cosmetic and pharmaceutical applications.

Abstract

Mango pulp is high in prebiotic dietary fibers vitamin c, polyphenols, carotenoids, vitamins and dietary minerals. Mint leaves are known for its medicinal aromatic and flavorsome qualities. Mint act as powerful antioxidant, protecting the body against the formation of cancerous cells and is rich source of polyphenolic compounds. Tulsi also have antioxidant, adaptogenic, anti-inflammatory, antibacterial and immune-enhancing properties. Honey is natural sweetener and has antiseptic and antibacterial properties. Medicinally, honey is non irritant promote rapid growth of healthy tissue and is useful in purities value, bed spores, skin and intestinal disorder, etc. Keeping in mind the beneficial effects of these raw materials, attempt was made to prepare value added drink from it, which may have medicinal value and good sensory acceptability with objective to prepare and evaluate the recipe for value added green mango-mint-tulsi beverage and to study the shelf life of the developed value added beverage. The formulation prepared with partial substitution of honey was more acceptable. Throughout the storage period, the squash prepared with honey scored more in flavor, taste, mouth-feel and overall acceptability than without honey. The cost of production without including labour, bottling and other overhead charges came out to be Rs. 34.5/L for squash without honey and Rs. 59.0/L for squash without honey.

In monetary terms, value-added’ per unit of the product is the margin between its selling price and cost of production. However, in the dairy and food industry, value addition refers to, extra, features of a product that are beyond the expectations of a consumer at little or no extra cost. In this context, the addition of value to milk can involve monetary value added to a liter of milk by turning it into milk products, enhancing the convenience of quality, shelf life, or health characteristics using processing and packaging interventions.

4.1. Conversion of Milk into Conventional Products

It is an age-old tradition to add value to milk by processing it into milk products. The primary aim was to increase the shelf life of milk that would otherwise be highly perishable. With inadequate facilities to preserve liquid milk fresh in the rural areas’ conversion of milk into a variety of milk products would be an ideal option to effectively preserve the huge quantities of milk procured during the flush season’ In the processing of milk products such as cream, butter, cheese and ghee, by-products such as skim milk, buttermilk, whey, and ghee residue are obtained’ Many of the dairy plants, particularly in India’ are usually confronted with the problem of by-products utilization, especially that of whey and ghee residue in an economical manner. Membrane separation processes have presented new possibilities for the production of newer intermediate products from dairy by-products that can be used for value addition in dairy and food industries.

Milk, as secreted in the udder of a healthy animal is practically sterile, but during its transit to the processing plant, becomes heavily contaminated with a variety of micro-organisms. These may be responsible for spoilage of milk or for causing diseases in animals and human beings. It thus becomes imperative that adequate heat treatment is given to milk to destroy these micro-organisms, thus increasing the shelf-life of milk as well as making it safe. Varied heat treatments offer the potential of producing different kinds of processed milk to satisfy the needs of the consumer. To cater to the ever-increasing demand of consumers, several varieties of processed milk are available in the market, some of which are discussed below.

5.1. Pasteurised Milk

Pasteurisation was named after the renowned French scientist Louis Pasteur, who discovered that spoilage organisms could be inactivated in wine by applying heat at temperatures below its boiling point. The process was later applied to milk and remains the most important operation in the processing of milk.

It is the process by which every particle of milk is heated to such temperature-time combinations (usually to 63°C for not less than 30 minutes or to 72°C for not less than 15 seconds) and promptly cooled to 4°C or below so as to destroy any harmful bacteria that may be present without affecting flavour and food value. Pasteurisation does not involve the use of any additives. Apart from making milk safe to drink, pasteurisation increases the length of time it can be kept before it spoils. The nutrient losses due to pasteurisation are so small, that they are considered insignificant.

Procedure: weigh vegetables 20 g (for each variation) Water: add sufficiently
1. Cook the vegetable in water in a pan according to the “cooking time” variations given in the table 1 and note down the color, texture and flavor changes takes place during cooking for each variation and record the observations

Introduction

A collaborative environment is necessary to enable a nurturing future for any business or entrepreneurship to succeed. While ventures may or may not have benefitted from collaborations, it is undoubtedly a step highly considered and regarded in any kind of field be it research, business, partnership between organizations or any international collaborations. The parties involved in these collaborations/partnerships share resources and risks as per agreed. The various kinds of models are discussed in this chapter ahead.

Venture Capital

Venture capital refers to financing that comes from companies or individuals in the business of investing in young, privately held businesses. They provide capital to young businesses in exchange for an ownership share of the business. Venture capital firms usually don’t want to participate in the initial financing of a business unless the company has management with a proven track record. Generally, they prefer to invest in companies that have received significant equity investments from the founders and are already profitable. Venture capital investors also prefer businesses that have a competitive advantage or a strong value proposition in the form of a patent, a proven demand for the product, or a very special (and protectable) idea. Startups with innovative ideas and technologies that have a great potential for wealth creation, but a high risk of failure might receive funding from venture capital. Typically, venture financing comes in the form of equity shares or a claim on equity that may arise in the future, such convertible debt, allowing the venture capital firm to acquire a portion of the company. New businesses are often the focus of venture capital, and Georges Doriot, a professor at Harvard Business School, is widely regarded as the “Father of Venture Capital.”

A five-kingdom classification system fails to classify viruses because they are not considered to be living. They neither reproduce independently nor utilize energy

General properties of virus

Viruses differ fundamentally from other classes of microorganism in that:

1.They are ultramicroscopic disease-producing entities.

2.They are simple in structure, basically composed of nucleic acid wrapped up in a protein coat.

3.The genome nucleic acid is either DNA or RNA , either single stranded or double stranded, but never both

4.Viruses have no metabolic activity of their own and lack enzyme systems and other constituents fundamental for independent growth and multiplication.

5.They do not posses ribosomes, nor transfer RNA and enzymes required for synthesis of nucleic acids and proteins.

6.Viruses are incapable of growth and division.

Vitamins are naturally occurring, vital nutrients that are needed in small amounts. They are important for bone and tissue health, wound healing, growth and development, immune system function, and other biological processes. These vital organic substances serve a variety of biochemical purposes. Vitamins, like minerals, are not biosynthesized by the body. Consequently, in order to maintain our health, we must obtain them from the food we eat or, in rare circumstances, supplements.

Vitamins in Milk

Together with water- soluble vitamins like vitamin C and B complex and provitamins, milk also contains a variety of fat- soluble vitamins like A, D, E, and K. Some of these vitamins do, however, have very low concentrations.

Vitamin A (Retinol)

Plant foods contain carotenoids, which are the precursors of vitamin A, whereas animal foods provide preformed vitamin A in the form of retinyl esters.

1. Introduction
All glassware used for bacteriological work must be meticulously cleaned and sterilized. Used and soiled glassware should be thoroughly washed to remove dirt and solid residues before drying and undergoing sterilization.
2. Cleaning of New Glassware
Borosilicate glass (e.g., Pyrex) and factory-washed soda glass require only standard washing before use. However, new, unwashed soda glass needs additional treatment to neutralize alkali content.
• Place the items (e.g., test tubes, Petri dishes, and flasks) in a suitable container, cover with a 1% tri-sodium phosphate solution, and bring to a boil.
• Remove the items, rinse with tap water, and immerse them in a 1% hydrochloric acid solution to neutralize alkalinity.
• Rinse thoroughly with tap water, followed by distilled water. Allow the glassware to dry before sterilization.
3. Cleaning of Used Glassware and Other Apparatus
• Autoclave all glassware contaminated by microorganisms (121°C for 15–20 minutes). This liquefies solid media, facilitating removal.
• Rinse the empty glassware with tap water, then warm water (50–55°C).
• Soak in a detergent solution (e.g., 1% washing soda or tri-sodium phosphate solution) and scrub with a brush.
• Rinse thoroughly with tap water to remove detergent traces, followed by distilled water.

Introduction

A variety of glassware, plasticware, and other apparatus are used for experimental purposes in a laboratory. They need to be washed, cleaned, dried, and sometimes sterilized before they can be used in the next set of experiments. Glassware needs special care of handling during washing, drying, sterilization, etc. because even a minor mistake in handling of glassware may result in its cracking/breakage and it may become unserviceable. All of these may result in a delay in experimentation if spare piece of the glassware is not available or it may affect serviceability of the associated apparatus.

A large number of glassware such as beaker, flask, measuring cylinder, burette, test tube, etc. are regularly used in a laboratory, which requires careful handling during the different stages of experiment as well as after completion of the experiment while preparing them for another set of experimentation.

Glass being a complex silicate, its properties depends on the type of silicate anions in the structure and the cations formed. Thermal properties of glass changes by the addition of boron oxide (B2 O3 ); hence, borosilicate glass is extensively used in the laboratories. With this brief background information, let us discuss about the necessity, procedures, and precautions during washing, cleaning, drying and sterilization of laboratory-wares. Figure 3.1 shows various advantages of using borosilicate glass in a laboratory.

Good laboratory practices require clean glassware, because an experiment may give erroneous result if dirty glassware is used at any step of the experiment. Hence, the glassware must be physically and chemically clean; and in many cases, it must be microbiologically clean or sterile. The commonly used glassware in a laboratory include test tube, measuring cylinder, pipettes, beakers, flasks, microscopic slides, etc.

Diverse categories of Food Products such as canning, preservation of fruits and vegetables, processing of poultry products, dairy goods and many more present different challenges for wastewater disposal and regular use of water. A significant source of environmental pollution is the dissipated water generated from food product development and other agricultural tasks. This waste is being considered the most strenuous and requires a high amount of capital to convert this waste into reusable waste and the main reason behind this is different nutritional components such as inorganic and organic carbon compounds, nitrogenous elements, suspended and dissolved solids, high amount of biochemical and chemical oxygen demands (BOD & COD).

    1.    Water activity (a_w) affects food chemistry and can be controlled by removal (dehydration or drying) or by chemically binding the water, reducing its activity. Water activity in foods ranges from 0.95 to 1.00 in fresh meats and vegetables to 0.20 in dried milk.

    2.    Water activity (a_w) may be defined in a number of ways, qualitatively, aw is a measure of unbound, free water in a system available to support biological and chemical reactions.

    3.    Water activity, not absolute water content, is what bacteria, enzymes, and chemical reactants encounter and is affected by at the micro environmental level in food materials.

    4.    Two foods with the same water content can have very different aw values depending upon the degree to which the water is free or otherwise bound to food constituents.

    5.    The term “water activity” is related to relative humidity. Relative humidity is defined as the ratio of the partial pressure of water vapour in the air to the vapour pressure of pure water at the same temperature.

    6.    Water activity or aw is a property of solutions and is the ratio of vapour pressure of the solution compared with the vapour pressure of pure water at the same temperature. Under equilibrium conditions water activity equals:

1. Natural Mineral Water- Food Safety & Standard Regulations - 2011 and IS 13428:2005 Reaff. 2009 requirements
2. Packaged Drinking Water other than Natural Mineral Water- Food Safety & Standard Regulations - 2011 and IS 14543:2004 Reaff. 2009 requirements
3. Water for processed Food Industry- IS 4251 : 1967 Reaff. 1992 requirements
4. Drinking water- IS 10500 : 2012

Introduction

Water is not only a vital environmental factor to all forms of life, but it has also a great role to play in socio-economic development of human population. Since antiquity humans have valued the importance of clean water and have used various techniques to improve its quality. In modern times, the safe drinking water became first a public health issue and then a human rights issue. The access to safe drinkin gwater now considered as a basic human right which is an essential component of effective policy for health protection. Improving access to safe drinking-water can result in tangible as well as intangible benefits to health. It has been shown that investments in water supply and sanitation can yield a net economic benefit, because the reductions in adverse health effects and healthcare costs outweigh the costs of undertaking the interventions (WHO, 2017). Past experiences have also proved that interventions in improving access to safe water favour the poor in particular, whether in rural or urban areas, and can be an effective part of poverty alleviation strategies. The disparity in water availability, reliability, and quality between the developed world and developing countries is enormous. Unfortunately, in many developing countries people lack access to safe water and therefore waterborne diseases are still a major cause of death, especially among children under age of 5. According to a joint report by World Health Organization (WHO) and United Nations International Children’s Emergency Fund (UNICEF) (WHO/UNICEF report 2017), around 2.1 billion people worldwide lack access to safe and readily available water at home, and around 4.5 billion, lack safely managed sanitation facilities. Moreover, many of these countries that have set drinking water standards often lack the resources to implement or enforce them.

Water is an essential component of all living things. Although once believed as a resource with a never-ending supply, it has now been understood that water is becoming increasingly scarce and therefore a proper management is necessary to keep a sustainable food production (Kannan & Anandhi, 2020). A continuous and safe supply of water is indispensable for the food industry where it has numerous applications along the agriculture and food production processes.

Water Requirements

Dairy plant requires large quantities of water on a daily basis for processing and cleaning purposes. Normally the specific water consumption ranges from 1000-5000 litres per 1000 litres of milk processed. The actual requirement can vary between dairies and is a function of 
    a)    Manufacturing process or manufacturing profile.
    b)    Auxiliary processes such as steam generation, design of the refrigeration processes and extent of social services.
    c)    Multiple use/reuse.
Water is a very valuable basic natural resource  and needs careful consumption. The water supply system in a dairy plant should be designed in such a way that consumption for each manufacturing department and auxiliary processes can be determined. This makes it possible to influence water consumption directly, and major losses can be determined very rapidly.

Introduction

In a research laboratory, we frequently need weigh chemicals and prepare different solutions of varying strength, as per the requirements. Accuracy in weighing and preparation of solution, with respect to weight/volume and strength, determines the success of experiment. In turn, the accuracy depends on weighing of solid chemical and/or measuring the volume of liquid chemical/making up the volume. In this Chapter, weighing of chemicals using appropriate weighing balance, preparation of solutions of different strength, their dilution, and precautions while preparing will be discuss in brief.

Weighing of Chemical

The primary method to determine the amount of chemicals to be used is weighing the chemical by using a balance.

In general, there are two types of balances to weigh chemicals in a laboratory:

1. Standard laboratory balance

2. Analytical balance

Standard Laboratory Balance

Generally, a standard laboratory balance is used for weighing a chemical when an error of ±0.02 g is acceptable. It is also used for pre-weighing, where approximate mass needs to be determined. Precise mass of the chemical is then determined using an analytical balance. Following are the steps in using a weighing balance in a laboratory.

When certain disease-causing bacteria or pathogens contaminate food, they can cause foodborne illness, often called “food poisoning”. Foods that are contaminated may not look, taste or smell any different from foods that are safe to eat. Salmonella, Campylobacter, Listeria and Escherichia coli are common bacteria causing foodborne illness. Some foodborne illnesses are mild where most people get better within a few days, but illness can sometimes be more severe and fatal. Bacillus cereus can form heat resistant spores. On cooking the food with these spores the heat resistant toxin is left at room temperature. Reheating or lightly cooking the food does not destroy these toxins. Most types of foodborne illnesses cause symptoms like nausea, vomiting, watery diarrhea, abdominal pain and cramps. Fever and/or the presence of blood or mucus in the stool are signs of bacterial infection that may require a medical consultation. Proper food handling is vital to prevent most foodborne illnesses and diseases. WHO has given keys towards safer food which should be followed. Unsafe food containing harmful bacteria, viruses, parasites or chemical substances, causes more than 200 diseases which range from diarrhoea to cancers. Foodborne and waterborne diarrhoeal diseases kill around 2 million people worldwide annually, including many children. A single foodborne bacterium can grow into more than two million bacteria in just seven hours under the right conditions. Foodborne pathogen called Listeria sp. can survive and sometimes grow on foods being stored in the refrigerator. Hepatitis A virus can cause long-lasting liver disease and spreads typically through raw or undercooked seafood or contaminated raw produce. It is estimated that more than 250 million people worldwide suffer food allergies. The boiling temperature (smoke point) of cooking oil is above 200 °C. The boiling point of water is 100 °C.

According to the World Health Day (WHO,2015) food contamination happens when food are corrupted with another substance. It can happen during the process of production, transportation, packaging, storage, sales and cooking process. The contamination can be physical, chemical and biological. Faulde et al., (2013) studied the infection caused to patients by mothfly

It is a discipline of science that describes handling, preparation and storage of food that can prevent food-borne diseases. When there are two or more cases of a similar illness from the ingestion of the same common food it is known as a food-borne disease outbreak (FSN, 2018). In order to avoid potential health hazards, food safety is very important. This needs safety between industry, market and the consumer. For food safety the labelling on food material is vital. This includes the origin of food, steps taken for food hygiene, food additives, pesticide residues, policies on biotechnology, food guidelines for the management of governmental import, export inspection and certification systems for foods. The food should be safe in the market with safe preparation and delivery of the food for the consumer. Foegeding et al., (1996) have done an assessment of risk factors with food borne pathogens.Warnock et al., (2007) have reported intervention to food safety.

When pathogens are in the food they may be transmitted to the consumer. It may result in an illness or death of the person or other animals. The pathogens include bacteria, viruses, molds and fungus. These multiply in the food medium. Joon et al., (2012) have found pathogenic bacteria in meat products of Korea. The developed countries have high intricate standards for the preparation of food; however, in the developing countries there are less standards and enforcement of the standards which are followed. Apart from food another major source of infection is water, which is very critical in spreading many diseases. It is possible to prevent food poisoning if good water is used for all purposes. However, it becomes difficult as in the food supply chain a large number of persons are involved apart from the fact that pathogens can be introduced into foods no matter how much precautions are taken. Cowden (2000) has studied the notification on food poisoning to improve health. Frenkel et al., (2015) studied the amount of wastes from food processing industries.

A process control system that identifies the hazardous points in the food production process is known as HACCP (Hazard Analysis Critical Control Point). It puts into place many stringent actions to prevent any hazards from occurring. In any food process if there is strict monitoring and controlling steps, the chances for hazards are minimum. Today, HACCP is an integral part of modern food industry that identifies and controls the major food risks. These are like microbiological, chemical and physical contaminants. These can also be practiced at home scale by checking proper storage, handling, cooking and cleaning procedures. These steps have resulted in industries taking greater responsibility for and controlling the food safety risk factors. This helps in better consumer protection, agriculture, the food processing industries and promotes domestic and international food trade.

The temperature of food storage is important. Below 4⁰C the food pathogens do not grow. Above 60⁰C some colonies begin to die. However, almost all die at 71⁰C. How hot is the food served is important for food safety. The size of the piece is important to how quickly it will cook all the way through. The smaller the piece the faster it will cook. Large or thicker pieces take a longer time to be cooked. Otherwise cooking for a longer time is better so that pathogenic microorganisms do not grow. In case of a thicker piece the color must be checked inside to outside after cooking which should be consistent. If the meat has the bone, check the color/temperature closest to the bone in the thickest part. In case of meat the contamination usually gets introduced while butchering and the practices used. The street food on a cart is perhaps the most contaminated as it has some level of bacteria in it. The meat in the decorated malls also has pathogenic bacteria. The environment plays a very pivotal role which can recontaminate the food even after proper cooking. The amount of harmful pathogens keeps growing in number with the passage of time. Fresh from the grill is the best, an hour or two is not ideal, half a day or overnight could make a person sick. Due to the reason that consumers do not know the time and temperature of cooking it is important to eat food which is freshly prepared.

Apart from the job content and specialization, the conditions or the atmosphere in which employees work is critical to their productivity. Work environment includes the tools and equipment provided to perform the job, the atmosphere at the work place, worker motivation etc.

Tools and Equipments

The quality of tools and equipment provided to a worker has a profound effect on the worker’s contribution to his job. A poorly designed tool can cause excessive physical burden on the worker and may result in fatigue. A faulty computer monitor can cause severe eyestrain, which might affect productivity in an office environment. Hence, tools and equipment should be appropriate for the production task. The design should consider ergonomic factors like grasping pressure, size, weight, ease of use etc. when designed properly, tools enable users to work efficiently, resulting in higher productivity. The equipment installed should be able to meet the capacity requirements of the job, have safety features and be easy to use. The use of the right tools and equipment can improve productivity in an organization substantially.

Yeasts

Yeasts are non filamentous, unicellular fungi that are typically spherical or oval. Like molds, yeasts are widely distributed in nature; they are frequently found as a white powdery coating on fruits and leaves.