eChapters

Introduction
In order to study the cultural, morphological and physiological characteristics of an individual species, a pure culture of the microorganisms is required. Several different methods of separating the microorganisms from a mixed culture are available. The two most frequently used methods involve making a pour plate and/or streak plate. Isolated colonies are obtained from individual organisms after incubation.
Streak Plate Method
Plating is generally applied to the inoculation of a medium in petridish. Streak plate method is best for the separation of organisms from a mixed culture. In this method, agar medium is allowed to solidify as a thin layer in a petri dish and culture is streaked on the surface of agar medium and allowed to incubate to get isolated colonies.
Materials
1. Sterile petri plate
2. Wire loop
3. Nutrient agar medium
4. Bunsen burner
5. Mixed culture of E.coli and Micrococcus leutus

21.1 Introduction
Khoa, as well as mawa or khava, is a partially dehydrated whole milk product that is produced by perpetually heating milk in a karahi over a direct fire while stirring and scraping it with a khunti until it reaches a semi-solid consistency. The pan is removed from the fire once the desired consistency is obtained, and the contents are transformed into a solid mass known as khoa pat. “Khoa is produced by rapid drying cow, buffalo, goat, or sheep milk, or a combination thereof, and must contain a minimum of 20% milk fat.The product is produced by heat desiccating milk to 65–69% solids in an open pan. Khoa has been utilised as a foundation for Indian desserts for centuries. Milk traders and halwais prepare it according to the conventional procedure. You typically require five times the amount of milk content.

21.2 Classification
There are three primary varieties: “pindi” for burfi, “dhap” for gulab jamun, pantooa, and other delicacies, and “danedar” for kalakand. Khoa production has been the most straightforward technique of preserving milk produced in rural areas during the flood season. Pindi, dhap, and Danedar are the three primary varieties of khoa that are prepared throughout the country. Khoa is the foundation for a diverse array of Indian delicacies, such as gulab jamun and other dishes.

1. Food Labelling

Food labelling is the primary means of communication between the producer and seller of food on one hand, and the purchaser and consumer on the other.

Basic Terminology in Food labelling

‘‘Food” means any substance, whether processed, semi-processed or raw, which is intended for human consumption, and includes drinks, chewing gum and any substance which has been used in the manufacture, preparation or treatment of “food” but does not include cosmetics or tobacco or substances used only as drugs.

‘‘Ingredient” means any substance, including a food additive, used in the manufacture or preparation of a food and present in the final product although possibly in a modified form.

Microscope

A microscope can be defined as an optical instrument consisting of a lens or a combination of lenses for getting magnified images of minute objects. A simple microscope is essentially an ordinary magnifying lens held on an adjustable stand. It can give magnification up to 20 times the size of the object.

A compound microscope consists of two separate lens system, the objective and eye piece (ocular) mounted on a holder known as body tube and with adjustment for illuminating the objects. It gives much greater magnification (100 to 1500 times) when compared to a simple microscope.

Lamination and coating techniques are integral to modern food packaging, providing additional layers of protection, functionality, and appeal to the packaging materials. These techniques enhance barrier properties, improve durability, and enable packaging to withstand a range of environmental and mechanical stresses. Lamination typically involves bonding multiple layers of materials to achieve specific characteristics, such as moisture resistance, heat stability, and strength. Coating, on the other hand, adds a surface layer that can enhance properties like grease resistance, sealability, and printability. This chapter explores the various lamination and coating techniques used in food packaging, examining their applications, benefits, and potential limitations.

    1.    Cereals are grown over 73% of the total world harvested area and contribute over 60% of the world food production providing dietary fibre, proteins, energy, minerals,  vitamins, b-glucans , Oligosaccharides, such as lactulose, fructo-oligosaccharides, transgalacto-oligosaccharides

    2.    Finger millet is a potent source of antioxidants and has potent radical-scavenging activity that is higher than that of wheat, rice, and other millets; these results corresponded to their phenolic content

    3.    The brown or red variety of finger millet, which is commonly available, had higher activity (94%) than the white variety (4%) using the DPPH method.

    4.    Most of the herbal plant polyphenols are known to have multi functional properties such as reducing agents, hydrogen donating antioxidants and singlet oxygen quenchers and flavonoids and their derivatives are the largest and most important group of polyphenols.

    5.    Phytosterols are found in plant foods and are structurally and functionally analogous to cholesterol in vertebrate animals. a-sitosterol, campesterol and stigmasterol are the most commonly occurring phytosterols and constitute 95% of total phytosterols in the diet.

2.1 Introduction
Typically, milk is obtained from domesticated animals (water buffalo, sheep, or goats) under farm-level circumstances. In smaller amounts, it can be utilized directly after boiling; but, in larger quantities, processing and storage are crucial. Farms provide their milk to milk vendors, who collect it in cans and deliver it to chilling centers or dairy processing plants. After milking, the milk is quickly chilled to 4°C, or it should be pasteurized and packaged within two hours. Before being marketed, milk must be pasteurized, with the exception of products that initially require raw milk or those that require direct application of heat, such as desiccation or another heating process. The market for novel goods and procedures is steadily growing. The main causes include rising disposable incomes, shifting consumer attitudes and concerns about safety, hygiene, and nutrition, and the entry of foreign companies.

2.2 Dairy plant requirements
The plant should be situated in an area free from waterlogging, well- protected, easily accessible by car, and equipped with a plentiful supply of drinkable water, a facility for disposing of waste, and energy.

2.2.1 Infrastructure requirements
For the receiving of milk, chilling, storing, separating, pasteurizing, homogenizing, packaging, and product manufacturing, civil works with appropriate space and machinery are needed. provisions for utility units such steam generation and refrigeration control, storage, and quality control. When choosing metals for equipment in dairy plants, look for materials that are nontoxic, tainting, corrosion-resistant, easy to clean, affordable, and long-lasting. Generally, alloys made of aluminum or 18:8 stainless steel are recommended.

Leavening agents produce carbon dioxide which helps to give characteristic structure to bread cakes and biscuits etc. In the case of cakes, leavening is produced by air entrapped in the dough due to the presence of egg in the mix. The important groups of leavening agent’s are l. baker’s yeast 2. Chemical leavening agents

8.1 Key legal statutes and regulatory foundations
Various policy measures were also undertaken by the Government of India in due course of time. The Government of India enacted four science policies after gaining independence, i.e. Scientific Policy Resolution (1958), Technology Policy Statement (1983), Science and Technology Policy (2003) and Science Technology and Innovation Policy (2013). The 1983 Technology Policy Statement possessed a simple goal of creating indigenous technology and boosting efficient absorption and adaptation of imported technologies suited to the nation’s priorities and. The Technology Policy Statement of 1983 was followed up through the establishment of the Technology Information Forecasting and Assessment Council (TIFAC), an autonomous organization entirely owned by the Government of India under the Department of Science and Technology during 1988. TIFAC also offers some funding and supports development and commercialization of technologies through the home-grown technology scheme, among its other operations. The Technology Development Board Act (1995) was also a significant policy move of the government in the same direction. Also in recent times, the Government of India also unveiled its own National IPR Policy 2016 with the motto cum slogan “Creative India, Innovative India”. In addition, the policy is aligned to a number of other schemes of the Indian Government like “Make in India”, “Atal Innovation Mission”, “Start-Up India” and “Stand-Up India”, all aimed at encouraging creativity, innovation and entrepreneurship in the nation.

1. Introduction

Livestock meat and fish are rich sources of high-quality protein and other valuable nutrients such as polyunsaturated fatty acids, vitamins, and minerals and are an essential part of our healthy diet (Vladau et al., 2008; Mahmud et al., 2018; Mohanti et al., 2019; Mei et al., 2019). Food fraud and adulteration is an age-old distortive practice. The rising incidence of food contaminants and adulterants-induced health issues has challenged consumers to identify and consume safe livestock meat and fish products. Its significance has increased in recent years due to global trade, awareness among consumers, and sociopolitical issues.

The nutritional richness and the high biological value of dairy milk have made it an important constituent of the balanced human diet. It is associated with better growth, improved status of some micronutrients, cognitive performance, motor development and activity. It is composed of a mixture of nutritive components as well as other bioactive factors such as biogenic amines, conjugated linoleic acid (CLA), with relevant physiological benefits. The consumption of dairy milk and products exert a protective action in human health and have been confirmed in preventing several chronic conditions like cardiovascular diseases (CVDs), some forms of cancer, obesity, immunomodulation and diabetes (Pereira, 2014). The chemical composition of bovine milk consists of 87% water, 4% to 5% lactose, 3% protein, 3% to 4% fat, 0.8% minerals, and 0.1% vitamins (Pereira, 2014).

1. Composition of Milk Fat

Bovine milk contains about 3.5 to 5% total lipid, existing as emulsified globules 2 to 4 μm in diameter and coated with a membrane, casein. The composition of milk fat is somewhat complex. Triglycerides constitute approximately 98% of milk fat, which is found in the milk globule, with the remainder being made up of di- and monoglycerides, phospholipids, cerebrosides, cholesterol, vitamins, tocopherols, carotene, and flavor components. Phospholipids are about 0.5 to 1% of total lipids, and sterols are 0.2 to 0.5%. These are mostly located in the milk fat globule membrane (Jensen et al., 1991). Cholesterol is the major sterol at 10 to 20 mg/dL.

INTRODUCTION

Food is the basic need of human beings. It gets spoiled primarily due to microorganisms and enzymes. Energy is required for microbial growth and enzymatic reactions. At low temperature, the rate of biochemical reactions is slow resulting in prolonged storage life of food. It must have been observed long back that food could be stored for longer period without deterioration during the winter months. Since then this principle is being used to extend the storage life of foods. A sea change can be observed in development of low temperature preservation technology. It has travelled a long journey to reach this stage; however, a major break through took place in twentieth century. Low temperature technology is of two types; refrigeration and freezing. In refrigeration storage, water is in liquid phase and temperature is maintained above freezing point. In freezing technology, the food is stored below freezing point and water gets converted into ice. The former is short term preservation due to slow growth of microbes and lower enzymatic activity while the later is long time preservation due to inactivation of enzymes during blanching and inhibition of microbial activity due to unavailability of free water and much lower temperature. Low temperature preservation is widely accepted and is of commercially important in food supply chain.

Low-temperature preservation is one of the most widely used physical approaches for extending food shelf life without seriously affecting quality. Reducing storage temperature slows microbial multiplication, enzyme activity and chemical deterioration, with inhibition increasing as temperature declines. Refrigeration maintains food above its freezing point, while freezing reduces temperature below zero and converts available water into ice. The chapter examines conventional refrigeration, advanced chilling, freezing, cryopreservation, superchilling and the influence of ice crystallisation on food quality. It explains how temperature, cooling rate and crystal size affect texture, nutritional retention and storage stability, while also addressing the limitations and quality changes associated with prolonged low-temperature storage.

A number of quality management tools are being used in food processing industry. Often selection of such tools are tough as it involves several factors for decision-making. The problem of choice of quality management and tools can be solved by selection on the basis of the objective decision making process rather than on the opinion of the decision makers (Sukkarn and Thawesaengskulthai, 2015).

Since time immemorial when man used primitive tools and machines to carry his loads, to draw his water, to till his land and to fabricate his building materials, he has been faced with the prospect of maintaining these assets until such time as he considered their useful life to be ended. Maintenance is very important to extend the useful life of an asset. Maintenance is usually viewed only as a repair function. It is, however, “a combination of any actions carried out to retain an item in, or restore it to, an acceptance condition”. In fact maintenance keeps or ensures that the entire production system is kept reliable, productive and efficient. All departments of a production system may have been designed beautifully without giving due consideration to maintenance management. The end result is obvious. Organizations like the National Productivity Council and others are playing a vital role in propagating the importance of Maintenance Management of all assets of the organization. In fact, all organizations must be having some assets, and hence the need for proper maintenance and ‘physical assets management’ which is synonymous with the word ‘Tero-technology’.

Introduction
Makkhan (Makhan) is an indigenous(desi) butter obtained by hand churning of dahi using wooden beater (mathani). It is traditional unsalted butter characterized by its delectable and rich flavour. It is white in colour with slightly greenish tinge and has typical soft body and a smooth grainy texture with pleasant aroma. This product has been extensively used for dietary and religious practices since vedic times. The entire quantity produced forms an intermediate product for preparation of ghee (clarified butter fat). Nearly 30% of the total milk production goes for desi butter manufacture. The average yield of the product is very low ranging from 5.5-7.0% for every 100 kg milk used for the purpose. The chemical composition of the product is variable depending upon the method of manufacture. It is difficult to achieve the uniform standard of 20% moisture in the final product due to limitations in the technique adopted for its manufacture. The non fatty solids and lactic acid are derived from the method of preparation and preservation. Higher non fatty solids in makhan indicates use of self soured milk, while a high lactic acid content indicates prolonged storage and in sanitary conditions of manufacture.

Whatever may be the nature and strength of resistance to the performance appraisal, management cannot relinquish this practice. Without employee appraisal, salary increases, reassignments or promotions cannot be administered rationally. Each of these areas has its specific problems and one of the solutions of modern times has been offered by an unusually promising frame work offered by Peter Drucker concept of Management by Objectives (MBO).
MBO calls on the subordinate to set his short-term performance goals in consultation with his superior. Goals explicitly state the actions which the employee will follow. Instead of being appraised in a traditional way by his superior, the subordinate appraises his own performance by evaluating it vis-à-vis the goals. Before establishing a goal, the individual studies his job, assesses his potentialities, and formulates some specific plans to reach the goal. The supervisor is not the evaluator, but directs the goal-setting process to ensure that it matches the objectives of the organization.

1. Introduction

Fermented milk products such as yoghurt are produced through out world (Kurmann et al., 1992). Yoghurt can be characterized as a gel like coagulated milk product, having a smooth consistency and a pleasing flavour and is now widely consumed both by infant and adult populations. It is common that yogurt preparation involves the use of equal amounts of two lactic acid bacteria (LAB): Streptococcus thermophillus (St) and Lactobacillus delbrueckii ssp. bulgaricus (Lb); samples prepared with these starter bacteria are labeled Lb-St (Ng et al., 2011). In the early stage of the incubation process, Lb stimulates the growth of St releasing amino acids from casein (where the most important is valine) and producing a moderate reduction in pH due essentially to St. Yoghurt has better digestibility than milk and this is related to its comparatively high content of free amino acids. Besides nutritional value, it possesses hypocholesterolaemic (Mann, 1977) and antitumour effects (Odd et al., 1983; Reddy et al., 1983).Yoghurt may be set or stirred and has a potential for being used as dietary supplement for infants. So they cross the line between dietary supplements, health foods and conventional foods with great facility. Now a days, emphasis is given on sweeteners and more natural sweeteners will be used (Hollingsworth, 2001). Natural sweeteners can be easily incorporated in yoghurt by blending cattle milk with fruit pulps like mango. The enhancement of other nutrients e. g. vitamins and minerals will be added advantage. Mango pulp which contains a high proportion of sugar, mostly monosaccharide, is easily adsorbed by the digestive systems (Celik and Bakirci, 2003).

Soybean is a comparatively inexpensive source of high quality protein. The protein and fat contents in soybean are above 40 % and 20 %, respectively. Nutritionally, soy protein is a more balanced plant protein for human consumption. Studies have indicated that consumption of soy protein decreases total serum cholesterol and minimizes risks for several cancers (Anderson et al., 1995; Messina, 1997; Zind, 1998). The positive health benefits associated with soybeans have greatly increased its consumer awareness which has created a large market for soy foods (Ohr, 2000; Liu, 2000). The problem of beany flavour in soy products can be overcome by lactic acid fermentation (Buono et al., 1990; Granata and Morr, 1996). Soy solids are not only abandoned source of protein but also have therapeutic properties against breast, prostate, colon and lung cancers due to its isoflavones like genistein and daidzein content. Fortification with soy made yoghurt beneficial in lowering cholesterol level and blood pressure and in relieving symptoms of menopause and osteoporosis.

Importance and Value of Human Resources

The employees are the human resources of an organization which are crucial for its functioning. The acquisition of human resources and their maintenance involves costs. Manpower costs include costs of research and designing of manpower programmes that is man-power planning, work study and designing of personnel policies etc. The costs of commissioning of man power, that is, costs of recruitment, induction, placement and training etc. The cost of maintenance of these resources is a very significant component of the total costs of an organization. Maintenance costs include wages and salaries, welfare and medical benefits, PF, gratuity and other retiring benefits etc. These costs form a substantial part of the total costs of an organization. In the last 30 years the treatment of manpower costs has been recognized as a separate division in accounting practices. The other assets like machinery, buildings etc come under depreciation. In contrast, human assets are the employees at all levels, as they grow in age and experience, add to their productivity and capacity to earn for the organization and become more valuable.