eChapters

Introduction

COVID-19, a worldwide pandemic, addresses a deflationary stun for the worldwide economy. The outbreak of coronavirus has had a significant impact on public health worldwide and all stages of various industries’ supply chain and value chain. The 2019 novel coronavirus (2019-nCoV) or the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is rapidly spreading from its origin in Wuhan City of Hubei Province of China to the rest of the world. The infection began in bats and was communicated to people by inhalation or contact with infected droplets, and its infection time frame goes from 2 to 14 days. Primary manifestations in hospitalized patients with COVID-19 include fever (98%), cough/hack (76%), and myalgia or weakness (44%). Severe complications include acute respiratory distress syndrome (ARDS), RNAemia, acute cardiac injury, and multiple organ failure. However, exaggerated immune responses may lead to Cytokine Storm. Cytokine storm syndrome includes ARDS, shock, and multi-organ failure (Zheng et al., 2020). Many people are asymptomatic also, and the severity of this disease varies from person to person. The fatality rate is estimated to range from 2 to 3%. Typical/low white cell checks can analyze it with raised C-responsive protein (CRP), periodic modernized tomographic chest examination. The infection spreads quicker than its two predecessors, the SARS-CoV and Middle East respiratory disorder Covid (MERS-CoV); however, it has lower casualty. The worldwide effect of this new scourge is yet questionable (Singhal, T. (2020).

Abstract

The present study was conducted to develop ready to eat extruded snack food based on germinated flour of wheat, Bengal gram and green gram. A Central Composite Rotatable Design (CCRD) for five variables at five levels was used for experimental combinations and optimization of the process parameters. Experimental design with green gram flour (5 to 20%) and bengal gram flour (1535%) in wheat flour, moisture content (14-18%), screw speed (200-300 rpm) and barrel temperature (126-134°C) as independent variables having 32 different combinations of snack formulations that were studied using response surface methodology to investigate the effect of these variables on product responses (Lateral expansion, bulk density, Water absorption index, water solubility index and sensory characteristics). Sensory analysis was carried out of all extrudates for texture, color and overall acceptability. Multiple regression equations were obtained to describe the effects of each variable on product responses. The optimum operating conditions for development of germinated wheat, green gram and Bengal gram flour extrudate were 132°C of barrel temperature, 275 rpm of screw speed, 17 % offeed moisture, 6.5% of green gram flour and 23.1% of Bengal gram flour. The responses predicted by the software for these optimum process conditions resulted lateral expansion of 169.59%, bulk density, 0.263g/cm³; water solubility index, 19.08; water absoption index, 13.88g/g; and overall acceptability value of 7.38.

Introduction

Whey is the serum or watery part of the milk that remains after separation of curd that result from the acid or proteolytic enzyme mediated co-agulation of milk. It is major by product of dairy industries manufacturing products like channa, paneer, cheese and chakka (Sharma and Bhatia, 1999).The current world production of whey is estimated at about 165 million tones, in that cheese whey accounts for nearly 95 % of total whey. In India near about one million tonne of whey is annually derived, in that major source of whey is from the production of channa, paneer and shrikhand production. This is mostly thrown away as a waste which creates environmental pollution, also in that way about 70, 000 tonnes of Nutritious solids are lost (Puranik, 1999).

11.1 Introduction

Fermented foods are the result of the activity of a few specialized types of micro-organisms among the thousands of species known to mankind. Success of such processes is depended upon the control of environmental conditions ensuring the growth of the desired microbial species and inhibition of others. The fermentation can be broadly defined as the process in which the carbohydrate or like compounds are broken down, under anaerobic or aerobic conditions [171]. The basic role of micro-organisms is to breakdown the organic compounds to get energy for their metabolic activities. From ancient times, people all over the world have developed traditional processes for production of alcoholic beverages (wine) either from natural sugar such as in wine or by degrading the starchy materials (beer). Fruit wines referred to as ‘Soma’ in our ancient scriptures have been part of food of man and were used as medicine, might have originated accidentally (transformation of fruit juice beverages having exhilarating or stimulating properties). Wines are made principally from fruits like grape or extract of other fruits of various types (table wine, sparkling wine, dessert, aperitif or vermouth and pop wines) while the beers are prepared from grains like barley, rice and corn. The principal carbohydrate in grain is starch which can not be fermented directly. This process is brought about by starch splitting enzymes by germinating the grains followed by drying and storage until needed. Distillation of beer produces a variety of liquors (whisky, rum, gin, vodka), while that of wine produces the brandy. Besides, the improvement in the raw material selection, fermentation conditions and techniques, a lot of waste material is generated during fermentation process in the industries. Nevertheless, the food based industries are significant contributors to the economy of the many countries of the world (Fig 1). The global market value of food fermentation based industries is estimated to be around 250 million US dollars. Interestingly, more than 10% of this accounts for alcoholic beverages (Fig 2) with projected increase of US$ 27 billions in 1989 to 40 billions in 1990 [93,123,123,129].

Lanolin, commonly known as Wool grease is primarily a lipid secretion from sebaceous glands of wool bearing animals. The role of Lanolin in nature is to protect wool and skin of animal from climate and the environment. It is an excellent emollient, self-emulsifying and dermatological safe for skin applications. Lanolin is a complex matrix since it is a mixture of high molecularweight esters of fatty acids with fatty alcohols, sterols, tri-terpene alcohols; diesters, long-chain alcohols & diols, long-chain fatty acids and hydroxy acids and is therefore difficult for effective matrix clean-up. Because of its water proofing and antimicrobial properties, lanolin is used mainly in cosmetic and pharmaceutical applications.

Abstract

Mango pulp is high in prebiotic dietary fibers vitamin c, polyphenols, carotenoids, vitamins and dietary minerals. Mint leaves are known for its medicinal aromatic and flavorsome qualities. Mint act as powerful antioxidant, protecting the body against the formation of cancerous cells and is rich source of polyphenolic compounds. Tulsi also have antioxidant, adaptogenic, anti-inflammatory, antibacterial and immune-enhancing properties. Honey is natural sweetener and has antiseptic and antibacterial properties. Medicinally, honey is non irritant promote rapid growth of healthy tissue and is useful in purities value, bed spores, skin and intestinal disorder, etc. Keeping in mind the beneficial effects of these raw materials, attempt was made to prepare value added drink from it, which may have medicinal value and good sensory acceptability with objective to prepare and evaluate the recipe for value added green mango-mint-tulsi beverage and to study the shelf life of the developed value added beverage. The formulation prepared with partial substitution of honey was more acceptable. Throughout the storage period, the squash prepared with honey scored more in flavor, taste, mouth-feel and overall acceptability than without honey. The cost of production without including labour, bottling and other overhead charges came out to be Rs. 34.5/L for squash without honey and Rs. 59.0/L for squash without honey.

In monetary terms, value-added’ per unit of the product is the margin between its selling price and cost of production. However, in the dairy and food industry, value addition refers to, extra, features of a product that are beyond the expectations of a consumer at little or no extra cost. In this context, the addition of value to milk can involve monetary value added to a liter of milk by turning it into milk products, enhancing the convenience of quality, shelf life, or health characteristics using processing and packaging interventions.

4.1. Conversion of Milk into Conventional Products

It is an age-old tradition to add value to milk by processing it into milk products. The primary aim was to increase the shelf life of milk that would otherwise be highly perishable. With inadequate facilities to preserve liquid milk fresh in the rural areas’ conversion of milk into a variety of milk products would be an ideal option to effectively preserve the huge quantities of milk procured during the flush season’ In the processing of milk products such as cream, butter, cheese and ghee, by-products such as skim milk, buttermilk, whey, and ghee residue are obtained’ Many of the dairy plants, particularly in India’ are usually confronted with the problem of by-products utilization, especially that of whey and ghee residue in an economical manner. Membrane separation processes have presented new possibilities for the production of newer intermediate products from dairy by-products that can be used for value addition in dairy and food industries.

Milk, as secreted in the udder of a healthy animal is practically sterile, but during its transit to the processing plant, becomes heavily contaminated with a variety of micro-organisms. These may be responsible for spoilage of milk or for causing diseases in animals and human beings. It thus becomes imperative that adequate heat treatment is given to milk to destroy these micro-organisms, thus increasing the shelf-life of milk as well as making it safe. Varied heat treatments offer the potential of producing different kinds of processed milk to satisfy the needs of the consumer. To cater to the ever-increasing demand of consumers, several varieties of processed milk are available in the market, some of which are discussed below.

5.1. Pasteurised Milk

Pasteurisation was named after the renowned French scientist Louis Pasteur, who discovered that spoilage organisms could be inactivated in wine by applying heat at temperatures below its boiling point. The process was later applied to milk and remains the most important operation in the processing of milk.

It is the process by which every particle of milk is heated to such temperature-time combinations (usually to 63°C for not less than 30 minutes or to 72°C for not less than 15 seconds) and promptly cooled to 4°C or below so as to destroy any harmful bacteria that may be present without affecting flavour and food value. Pasteurisation does not involve the use of any additives. Apart from making milk safe to drink, pasteurisation increases the length of time it can be kept before it spoils. The nutrient losses due to pasteurisation are so small, that they are considered insignificant.

Procedure: weigh vegetables 20 g (for each variation) Water: add sufficiently
1. Cook the vegetable in water in a pan according to the “cooking time” variations given in the table 1 and note down the color, texture and flavor changes takes place during cooking for each variation and record the observations

Introduction

A collaborative environment is necessary to enable a nurturing future for any business or entrepreneurship to succeed. While ventures may or may not have benefitted from collaborations, it is undoubtedly a step highly considered and regarded in any kind of field be it research, business, partnership between organizations or any international collaborations. The parties involved in these collaborations/partnerships share resources and risks as per agreed. The various kinds of models are discussed in this chapter ahead.

Venture Capital

Venture capital refers to financing that comes from companies or individuals in the business of investing in young, privately held businesses. They provide capital to young businesses in exchange for an ownership share of the business. Venture capital firms usually don’t want to participate in the initial financing of a business unless the company has management with a proven track record. Generally, they prefer to invest in companies that have received significant equity investments from the founders and are already profitable. Venture capital investors also prefer businesses that have a competitive advantage or a strong value proposition in the form of a patent, a proven demand for the product, or a very special (and protectable) idea. Startups with innovative ideas and technologies that have a great potential for wealth creation, but a high risk of failure might receive funding from venture capital. Typically, venture financing comes in the form of equity shares or a claim on equity that may arise in the future, such convertible debt, allowing the venture capital firm to acquire a portion of the company. New businesses are often the focus of venture capital, and Georges Doriot, a professor at Harvard Business School, is widely regarded as the “Father of Venture Capital.”

A five-kingdom classification system fails to classify viruses because they are not considered to be living. They neither reproduce independently nor utilize energy

General properties of virus

Viruses differ fundamentally from other classes of microorganism in that:

1.They are ultramicroscopic disease-producing entities.

2.They are simple in structure, basically composed of nucleic acid wrapped up in a protein coat.

3.The genome nucleic acid is either DNA or RNA , either single stranded or double stranded, but never both

4.Viruses have no metabolic activity of their own and lack enzyme systems and other constituents fundamental for independent growth and multiplication.

5.They do not posses ribosomes, nor transfer RNA and enzymes required for synthesis of nucleic acids and proteins.

6.Viruses are incapable of growth and division.

Vitamins are naturally occurring, vital nutrients that are needed in small amounts. They are important for bone and tissue health, wound healing, growth and development, immune system function, and other biological processes. These vital organic substances serve a variety of biochemical purposes. Vitamins, like minerals, are not biosynthesized by the body. Consequently, in order to maintain our health, we must obtain them from the food we eat or, in rare circumstances, supplements.

Vitamins in Milk

Together with water- soluble vitamins like vitamin C and B complex and provitamins, milk also contains a variety of fat- soluble vitamins like A, D, E, and K. Some of these vitamins do, however, have very low concentrations.

Vitamin A (Retinol)

Plant foods contain carotenoids, which are the precursors of vitamin A, whereas animal foods provide preformed vitamin A in the form of retinyl esters.

1. Introduction
All glassware used for bacteriological work must be meticulously cleaned and sterilized. Used and soiled glassware should be thoroughly washed to remove dirt and solid residues before drying and undergoing sterilization.
2. Cleaning of New Glassware
Borosilicate glass (e.g., Pyrex) and factory-washed soda glass require only standard washing before use. However, new, unwashed soda glass needs additional treatment to neutralize alkali content.
• Place the items (e.g., test tubes, Petri dishes, and flasks) in a suitable container, cover with a 1% tri-sodium phosphate solution, and bring to a boil.
• Remove the items, rinse with tap water, and immerse them in a 1% hydrochloric acid solution to neutralize alkalinity.
• Rinse thoroughly with tap water, followed by distilled water. Allow the glassware to dry before sterilization.
3. Cleaning of Used Glassware and Other Apparatus
• Autoclave all glassware contaminated by microorganisms (121°C for 15–20 minutes). This liquefies solid media, facilitating removal.
• Rinse the empty glassware with tap water, then warm water (50–55°C).
• Soak in a detergent solution (e.g., 1% washing soda or tri-sodium phosphate solution) and scrub with a brush.
• Rinse thoroughly with tap water to remove detergent traces, followed by distilled water.

Introduction

A variety of glassware, plasticware, and other apparatus are used for experimental purposes in a laboratory. They need to be washed, cleaned, dried, and sometimes sterilized before they can be used in the next set of experiments. Glassware needs special care of handling during washing, drying, sterilization, etc. because even a minor mistake in handling of glassware may result in its cracking/breakage and it may become unserviceable. All of these may result in a delay in experimentation if spare piece of the glassware is not available or it may affect serviceability of the associated apparatus.

A large number of glassware such as beaker, flask, measuring cylinder, burette, test tube, etc. are regularly used in a laboratory, which requires careful handling during the different stages of experiment as well as after completion of the experiment while preparing them for another set of experimentation.

Glass being a complex silicate, its properties depends on the type of silicate anions in the structure and the cations formed. Thermal properties of glass changes by the addition of boron oxide (B2 O3 ); hence, borosilicate glass is extensively used in the laboratories. With this brief background information, let us discuss about the necessity, procedures, and precautions during washing, cleaning, drying and sterilization of laboratory-wares. Figure 3.1 shows various advantages of using borosilicate glass in a laboratory.

Good laboratory practices require clean glassware, because an experiment may give erroneous result if dirty glassware is used at any step of the experiment. Hence, the glassware must be physically and chemically clean; and in many cases, it must be microbiologically clean or sterile. The commonly used glassware in a laboratory include test tube, measuring cylinder, pipettes, beakers, flasks, microscopic slides, etc.

Diverse categories of Food Products such as canning, preservation of fruits and vegetables, processing of poultry products, dairy goods and many more present different challenges for wastewater disposal and regular use of water. A significant source of environmental pollution is the dissipated water generated from food product development and other agricultural tasks. This waste is being considered the most strenuous and requires a high amount of capital to convert this waste into reusable waste and the main reason behind this is different nutritional components such as inorganic and organic carbon compounds, nitrogenous elements, suspended and dissolved solids, high amount of biochemical and chemical oxygen demands (BOD & COD).

    1.    Water activity (a_w) affects food chemistry and can be controlled by removal (dehydration or drying) or by chemically binding the water, reducing its activity. Water activity in foods ranges from 0.95 to 1.00 in fresh meats and vegetables to 0.20 in dried milk.

    2.    Water activity (a_w) may be defined in a number of ways, qualitatively, aw is a measure of unbound, free water in a system available to support biological and chemical reactions.

    3.    Water activity, not absolute water content, is what bacteria, enzymes, and chemical reactants encounter and is affected by at the micro environmental level in food materials.

    4.    Two foods with the same water content can have very different aw values depending upon the degree to which the water is free or otherwise bound to food constituents.

    5.    The term “water activity” is related to relative humidity. Relative humidity is defined as the ratio of the partial pressure of water vapour in the air to the vapour pressure of pure water at the same temperature.

    6.    Water activity or aw is a property of solutions and is the ratio of vapour pressure of the solution compared with the vapour pressure of pure water at the same temperature. Under equilibrium conditions water activity equals:

1. Natural Mineral Water- Food Safety & Standard Regulations - 2011 and IS 13428:2005 Reaff. 2009 requirements
2. Packaged Drinking Water other than Natural Mineral Water- Food Safety & Standard Regulations - 2011 and IS 14543:2004 Reaff. 2009 requirements
3. Water for processed Food Industry- IS 4251 : 1967 Reaff. 1992 requirements
4. Drinking water- IS 10500 : 2012

Introduction

Water is not only a vital environmental factor to all forms of life, but it has also a great role to play in socio-economic development of human population. Since antiquity humans have valued the importance of clean water and have used various techniques to improve its quality. In modern times, the safe drinking water became first a public health issue and then a human rights issue. The access to safe drinkin gwater now considered as a basic human right which is an essential component of effective policy for health protection. Improving access to safe drinking-water can result in tangible as well as intangible benefits to health. It has been shown that investments in water supply and sanitation can yield a net economic benefit, because the reductions in adverse health effects and healthcare costs outweigh the costs of undertaking the interventions (WHO, 2017). Past experiences have also proved that interventions in improving access to safe water favour the poor in particular, whether in rural or urban areas, and can be an effective part of poverty alleviation strategies. The disparity in water availability, reliability, and quality between the developed world and developing countries is enormous. Unfortunately, in many developing countries people lack access to safe water and therefore waterborne diseases are still a major cause of death, especially among children under age of 5. According to a joint report by World Health Organization (WHO) and United Nations International Children’s Emergency Fund (UNICEF) (WHO/UNICEF report 2017), around 2.1 billion people worldwide lack access to safe and readily available water at home, and around 4.5 billion, lack safely managed sanitation facilities. Moreover, many of these countries that have set drinking water standards often lack the resources to implement or enforce them.

Water is an essential component of all living things. Although once believed as a resource with a never-ending supply, it has now been understood that water is becoming increasingly scarce and therefore a proper management is necessary to keep a sustainable food production (Kannan & Anandhi, 2020). A continuous and safe supply of water is indispensable for the food industry where it has numerous applications along the agriculture and food production processes.

Water Requirements

Dairy plant requires large quantities of water on a daily basis for processing and cleaning purposes. Normally the specific water consumption ranges from 1000-5000 litres per 1000 litres of milk processed. The actual requirement can vary between dairies and is a function of 
    a)    Manufacturing process or manufacturing profile.
    b)    Auxiliary processes such as steam generation, design of the refrigeration processes and extent of social services.
    c)    Multiple use/reuse.
Water is a very valuable basic natural resource  and needs careful consumption. The water supply system in a dairy plant should be designed in such a way that consumption for each manufacturing department and auxiliary processes can be determined. This makes it possible to influence water consumption directly, and major losses can be determined very rapidly.