eChapters

a) A Key GDP Contributor: Dairy and Livestock sector form the backbone of agriculture with 28 per cent contribution in GDP and the progress in this sector is dependent on innovative measures to increase the value for farmers as well as customers.

b) Doubling Farmer’s Income : Doubling Farmer’s Income by 2022 can be achieved by improving crop and livestock productivity, efficiency to utilize resources, increased intensity of cultivation and diversification in high value cash crops, processing and value addition of food and animal products. This price realization can be assured through market reforms and efficient market linkages.

c) Unorganised sector – a challenge : The Indian dairy sector is still characterized by strong presence of unorganized sector. Apart from the Cooperative dairy sector, in the last few years there has been a considerable growth in Private Dairy sector and Milk Producers Organization like Maahi in Gujarat. If small dairy farmers are provided with sufficient ‘value addition’ training and support, they can increase their income considerably.

d) Value addition : Further, the milk processing plants can also go for value addition and innovation and in turn pay remunerative prices to milk producers.

Food preservation has evolved from traditional household practices to an interdisciplinary field incorporating microbiology, biochemistry, engineering, biotechnology, packaging, nanotechnology and digital intelligence. The book demonstrates that food stability can increasingly be achieved without excessive reliance on synthetic chemical preservatives. Effective preservation requires control of microbial growth, enzyme activity, oxidation, moisture, temperature and environmental exposure while protecting sensory and nutritional quality. Traditional practices remain scientifically valuable, while thermal, non-thermal, biological, packaging and digital technologies offer new possibilities. Future food systems will depend on carefully designed combinations of these approaches to improve safety, shelf life, sustainability, traceability and consumer acceptance while overcoming cost, regulatory and scalability challenges.

For looking into the biosafety in research laboratories, agriculture and other food producing industries it is important to look into the numerous strains of lactic acid bacteria that are associated with food systems and are capable of producing bacteriocin or antibacterial proteins with activity against foodborne pathogens such as Listeria monocytogens, Staphylococcus aureus and Clostridium botulinum. Recently considerable attention has been given on the identification of these proteins and their biochemical properties including spectrum of activity, production conditions, purification procedures, aminoacid composition and mode of action. Advances in genetic engineering have facilitated the characterization of proteins at genetic level, providing information about protein synthesis, structure and cloning of bacteriocin genes into other organism. One problem in the use of bacteriocin in their applications as bio-preservatives is the emergence of resistant population. The understanding on the mechanism of resistance to bacteriocins is limited which limits the application in biopreservation. Much of the work on bacteriocin has been done under controlled laboratory conditions without the presence of adventitious microbial population. More research is needed to determine the efficacy of bacteriocin producing organisms and bacteriocins which are stable under commercial conditions. Foodborne illness is a major international problem and an important cause of reduced economic growth. The contamination of the food supply with the pathogens and its persistence, growth, multiplication and/or toxin production

A sustainable and healthy food system is one which can provide food and nutrition security for all, in an economical way which is socially and environmentally sound for future generations. Underutilized indigenous and traditional crops can support and strengthen the existing food system, as they are in particular economical and friendly with the environment. Several underutilized indigenous and traditional crops are nutrient dense and adapted to marginal conditions, suggesting that they could be used to champion sustainable and resilient agriculture and food systems for smallholder farmers residing in these environments. The mainstreaming of underutilized indigenous and traditional crops and foods should be recognized for their attributes to make them desirable and to conserve them. In this regard, the fact that value chains for underutilized indigenous and traditional crops are poorly developed at present has created many opportunities to diversify the current food systems. This will create a new employment, market and distribution opportunities.It will also help to combat poverty. It will be if the smallholder farmers and women in rural areas are given the opportunity to utilize underutilized indigenous and traditional crops and re-enter the manistream system. A significant role which is played by the women for the production and conservation of underutilized indigenous and traditional crops offers opportunities for women empowerment through their inclusion in the food system. Promotion of gender equality and empowerment of women is directly linked for strengthening the sustainable food systems that can fight hunger and malnutrition and improve the lives and livelihoods of rural populations. The mainstreaming of underutilized indigenous and traditional crops into the food system would support women to diversify in a sustainable way, feed their own households and to provide nutritious food into the local markets. This will also help in addressing, the Sustainable

Introduction

India’s rural economy has benefited greatly from the dairy sector. It has played a significant role in bringing about socioeconomic change. India’s dairy industry has undergone significant change as a result of the White Revolution. The “White Revolution” can be credited with increasing milk availability and providing rural India with a stable source of employment and income. The business has drastically changed to meet India’s increasing demand for milk and milk products. India is currently the world’s greatest producer of milk. The Operation Flood Program is credited with this title. The dairy market in India is projected to increase at a compound annual growth rate (CAGR) of 6.61% from 2024 to 2029, from an estimated US$26.11 billion in 2024 to US$35.96 billion by 2029 (Indus Food, 2024). With over 8 crore farmers employed and a 5% national GDP contribution, the dairy industry in India is vital to the country’s economy. With around 25% of the world’s milk supply and a massive livestock population of 536.76 million animals, it tops the world in milk production. With major markets in the United Arab Emirates, Saudi Arabia, the United States, Singapore, and Bhutan, India exports approximately 63,738.47 metric tonnes of dairy products in 2023–2024, generating US$ 272.64 million.

Microbial contamination sources: Foods may be contaminated by microorganisms at any time during harvest, storage, processing, distribution, handling, or preparation. The primary sources of microbial contamination are soil, air, animal feed, animal hides and intestines, plant surfaces, sewage, and food processing machinery or utensils. Since most foods either carry or eventually acquire bacteria, molds, or yeasts, microorganisms are the major causes of food spoilage. The increased load of microorganisms leads to rapid spoilage.

Organisms: Bacteria and fungi (yeasts and molds) are the principal types of microorganisms that cause food spoilage and food- borne illnesses. The typical microorganisms that cause food spoilage are bacteria (e.g., Pseudomonas), yeasts (e.g., Candida), and molds (e.g., Rhizopus). The growth of microorganisms in the food will increase the load of microorganisms, and the heavier load will add to the difficulty of preventing spoilage and may influence the kind of spoilage to be anticipated.

Food spoilage may be defined as any change that renders food unfit for human consumption. These changes may be caused by various factors, including contamination by microorganisms, infestation by insects, or degradation by endogenous enzymes (those present naturally in the food). In addition, physical and chemical changes, such as the tearing of plant or animal tissues or the oxidation of certain constituents of food, may promote food spoilage. Foods obtained from plant or animal sources begin to spoil soon after harvest or slaughter. The enzymes contained in the cells of plant and animal tissues may be released as a result of any mechanical damage inflicted during postharvest handling. These enzymes begin to break down the cellular material. The chemical reactions catalyzed by the enzymes result in the degradation of food quality, such as the development of off- flavours, the deterioration of texture, and the loss of nutrients. The enzymatic changes in foods are desirable to some extent as in the case of ripening of fruits, but continued enzymatic activity beyond an optimum point becomes food deterioration due to rotting. The native enzymes may be inactivated by heat, radiation or by the use of specific chemicals.

Tea as a Beverage

Tea is the most popular beverage consumed in the world after water. All types of teas including black. green, oolong, white and pu-erh tea are mainly made from the buds and young leaves of tea plants, Camellia sinensis (L.) O. Kuntze belongs to the family Theaceae [1]. Depending on the different processing techniques of tea leaves, tea types are categorized as “Green Tea” (unfermented), “Black Tea” (fully fermented), “Oolong Tea” (partially fermented), “Pu-ErhTea” (post fermented) and white tea (unfermented) [2]. The manufacturing process for these different types of teas mainly differ in the degree of enzymatic oxidation (fermentation) process [1].

Introduction

Coffee is a beverage made from the roasted and ground seeds of the African tropical evergreen coffee plant. Coffee is one of the world’s three most popular beverages (together with water and tea), as well as one of the most profitable international commodities, and it is the world’s second most marketed product after petroleum (Myhrvold, 2021).Coffee is a popular beverage that is enjoyed around the world for its taste, health benefits, and ability to enhance physical performance (Simkin, 2008).

16.1. CANNED FOODS-PRESERVATION

Canning is a method of preserving food from spoilage by storing it in containers that are hermetically sealed and then sterilized by heat. The process was invented after prolonged research by Nicolas Appert of France in 1809, in response to a call by his government for a means of preserving food for army and navy use. Appert’s method consisted of tightly sealing food inside a bottle or jar, heating it to a certain temperature, and maintaining the heat for a certain period, after which the container was kept sealed until use. It was 50 years before Louis Pasteur was able to explain why the food so treated did not spoil: the heat killed the microorganisms in the food, and the sealing kept other microorganisms from entering the jar. Samuel C. Prescott and William Underwood of the United States set canning on a scientific basis by describing specific time-temperature heating requirements for sterilizing canned foods.

Most vegetables, fruits, meat and dairy products, and processed foods are stored in tin cans, but soft drinks and many other beverages are now commonly stored in aluminum cans, which are lighter and do not rust. Aluminum cans are made by impact extrusion; the body of the can is punched out in one piece from a single aluminum sheet by a stamping die. This seamless piece, which has a rounded bottom, is then capped with a second piece as its lid. The tabs used in pop- top cans are also made of aluminum. Bimetal cans are made of aluminum bodies and steel lids.

Canneries are usually located close to the growing areas of the product to be packed, since it is desirable to can foods as quickly as possible after harvesting. The canning process itself consists of several stages: cleaning and further preparing the raw food material; blanching it; filling the containers, usually under a vacuum; closing and sealing the containers; sterilizing the canned products; and labeling and warehousing the finished goods. Cleaning usually involves passing the raw food through tanks of water or under high- pressure water sprays, after which vegetable or other products are cut, peeled, cored, sliced, graded, soaked, pureed, and so on. Almost all vegetables and some fruits require blanching by immersion in hot water or steam; this process softens the vegetable tissues and makes them pliable enough to be packed tightly, while also serving to inactivate enzymes that can cause undesirable changes in the food before canning. Blanching also serves as an additional or final cleansing operation.

10.1 CEREALS AND CEREAL PRODUCTS

CONTAMINATION: The sources of contamination in cereals and cereal products are soil, insects, air, etc. Most of the microorganisms are removed with the outer portions of the grain during milling. The bleaching agents used during milling also reduce numbers of microorganisms. The bacteria in wheat flour include Bacillus, Micrococcus, Flavobacterium. Mold spores are chiefly of Aspergillus spp and Penicillium spp and in addition Alternaria, Cladosporium and other genera are found. The numbers of microbes in cereals and cereal products vary widely from few hundred per gram to million. Corn meal contains several hundred to several thousand bacteria and mold per gram.

14.1. EGGS

India is the third largest producer of eggs. Fresh eggs are gathered on automatic collection belts at the farm and stored in a cooler at about 7°C. The eggs are then delivered to a central processing plant, where they are washed, sanitized, and graded. Grading involves the sorting of eggs into size and quality categories using automated machines. Flash candling (passing the eggs over a strong light source) detects any abnormalities such as cracked eggs and eggs containing bloodspots or other defects. Higher-grade eggs have a thick, upstanding white, an oval yolk, and a clean, smooth, unbroken shell.

14.1.1. Eggs Contamination

More than 90 percent of all eggs are free of contamination at the time they are laid; contamination with Salmonella bacteria and with certain spoilage organisms occurs essentially afterward. They become contaminated by faecal matter from the hen, by the lining of nest, by handling, by wash water and packing materials. The total number of microorganisms per shell of a hen’s egg was found to range from 100 to 10,000,000 with a mean of about 100,000. Proper washing and sanitizing of eggs eliminates most Salmonella and spoilage organisms deposited on the shell. The organism Salmonella enteritidis, a common cause of gastroenteritis (a form of food poisoning), has been found to be transferred through the hen ovary in fewer than 1 percent of all eggs produced. Ovarian- transferred S. enteritidis can be controlled by thorough cooking of eggs (i.e., until there are no runny whites or yolk).

13.1. CONTAMINATION

MICROFLORA: The flora of living fish depends upon the microbial contents of the waters in which they live. The slime in the outer surface of the fish contain bacteria of the genera: Pseudomonas, Alcaligenes, Micrococcus, Flavobacterium, Corynebacetrium, Bacillus, Sarcina, Serratia and Vibrio. The same species of bacteria are found in shrimps, crabs, lobsters and similar sea foods. Oysters and Shell fish that pass large amount of water through their bodies, pick-up soil and water microorganisms in this way including human pathogens. Common spoilage microorganisms of fish include species of Pseudomonas, Moraxella, and Acinetobacter, found mainly in marine fish, and Bacillus and Micrococcus, found in freshwater fish. Fish may also contain pathogenic (disease-causing) microorganisms such as Salmonella and Escherichia coli. Pathogenic contamination is of special concern with mollusks because they are often eaten raw and as whole animals.

SOURCES: Boats, boxes, bins, fish houses and fishermen are the sources of contamination of these bacteria. The bacterial population ranges from few hundred to few million per square centimeter. Because of their soft tissues and aquatic environment, fish are extremely susceptible to microbial contamination. At the time of harvest, fish carry a high microbial load on the surface of their skin, in their intestinal tract, and in their gills. The type and number of microorganisms that live in fish vary according to the season, the species, and the natural habitat. Additional contamination may occur during the harvesting, handling, or processing of the fish. Harvested fish must be immediately stored in a low-temperature environment such as ice or refrigerated seawater. This chilling process slows the growth of microorganisms that live in fish and inhibits the activity of enzymes. Because fish have a lower body temperature, softer texture, and less connective tissue than land animals, they are much more susceptible to microbial contamination and structural degradation. If immediate chilling is not possible, then the fish must generally be sold and eaten on the day of the harvest.

11.1. FRUITS-Maturation, Ripening and Storage

Fruits are natural foods containing substantial quantities of essential nutrients and the best sources of minerals like sodium, potassium and magnesium, vitamins (antioxidants), such as vitamin A, C and enzymes. They are rich in fructose and water. Fruits are living biological entities that perform a number of metabolic functions. Two functions of particular importance in fruit processing are respiration (the breaking down of carbohydrates, giving off carbon dioxide and heat) and transpiration (the giving off of moisture). Senescence is the period when chemical synthesizing pathways give way to degradative processes, leading to aging and death of tissue. Fruit ripening is thus the result of many complex changes, some interactive but many independent of one another. Once the fruit is harvested, respiration and transpiration continue, but only for as long as the fruit can draw on its own food reserves and moisture. It is this limited ability to continue vital metabolic functions that defines fruit as perishable.

CONTAMINATION

The healthy inner flesh of meat contains few or no microorganisms, although they have been found in lymph nodes, bone marrow and intestine. Staphylococcus, Streptococcus, Clostridium and Salmonella have been isolated from lymph nodes of red meat animals. During slaughtering the lymph nodes are removed from edible parts. The important contamination, however comes from external sources during bleeding, handling and processing. During bleeding, skinning and cutting the main sources of microorganisms are from the exterior of the animal- hide, hoof and hair and the intestinal tract. The exterior of the animal harbours large number and many kinds of microorganisms from soil, water, feed and manure as well as its natural surface flora.

Knives, cloths, air and hands and clothing of workers can serve as intermediate sources of contamination. During handling and processing the contamination can come from containers, air, other contaminated meat, equipments and personnel.

Molds that grow on the surface of the meat include the species of Cladosporium, Sporotrichum, Geotrichum, Mucor, Monilia, Penicillium and Alternaria. The important bacteria found in meat are Staphylococcus, Sarcina, Streptococcus, Clostridium, Leuconostoc, Lactobacillus, Proteus, Flavobacterium, Bacillus, Streptomyces, Escherichia and Salmonella.

15.1 CONTAMINATION

Milk is sterile at secretion in the udder, but contaminated by bacteria when it leaves the udder of a healthy cow. Milk is subject to contamination from the animal and adjacent areas. Bacteria found in manure, soil and water may enter milk from these sources. Certain diseases may be transmitted through milk, from a cow. It is essential that cows producing milk should be free from any contagious or infectious disease. Bovine tuberculosis comes from consumption of milk of tuberculous cows. Tuberculosis, brucellosis, mastitis and actinomycosis are the human disease transmitted through milk. The disease producing bacteria may find their way into milk and cause infectious diseases in children and adults.

COMMON BACTERIA IN MILK: The common bacteria found in milk are: Rickettsia, Coxiella burnetii, lactic acid bacteria, Lactobacillus bulgaricus, Streptococcus thermophilus, aerobic rods, Bacillus subtilis, Bacillus cereus, anaerobic bacteria, Clostridium butyricum .

Sources of Microbial contamination: Milk contains relatively few bacteria when it leaves the udder of a healthy cow. Milk is subject to contamination from the animal udder, skin and adjacent areas barn yard, milk utensils, milkers. Bacteria found in manure, soil and water may enter milk from these sources.

Definition

It is one of the important portion of milk which forms a fatty layer on top of milk when left un-disturbed. It is a prime component of milk and comprises mainly of milk fat. It may be defined as “that portion of milk which is rich in milk fat” meaning portion of milk into which larger portion of milk fat has been gathered.  According to FSSAI, 2011 Malai and Cream are defined as –

Malai means the product rich in butter fat prepared by boiling and cooling cow or buffalo milk or a combination thereof. It shall contain not less than 25.0 per cent milk fat.

16.1 Introduction
The fluid product “Cream” is defined by FSSAI standards as an emulsion of fat-in-skimmed milk that is comparatively rich in fat. It is derived by physical separation from cow milk, buffalo milk, or milk of any other species as defined under this regulation, or a mixture thereof. Cream, with the exception of sterilized variants, is composed of a minimum of 25% milk fat and is derived from cow or buffalo milk or a combination of both. It is a high-energy source that is abundant in fat and fat-soluble vitamins A, D, E, and K. The vitamin content is contingent upon the fat percentage in the cream.
16.2 Classification
Cream may be broadly classified into Market cream (direct consumption) and Manufacturing cream (manufacture of dairy products). The three different types of cream are:
i. Table cream/Light cream contains 20-25% milk fat.
ii. Coffee cream/ Whipping cream Contains 30-40 % milk fat, and
iii. Heavy cream/Plastic cream contains 65-85 % milk fat.
As per FSSAI Standards, Cream may be labeled according to milk fat content (m/m) along with product name as specified in item 1 of this Standard, as follows:
i. Low fat cream: Minimum 10 per cent. and less than 40 per cent.;
ii. Medium fat cream: Minimum 40 per cent. and less than 60 per cent.;
iii. High fat cream: Minimum 60 per cent.

Butter is one of the leading milk product in developed countries of the world. It serves as a balance wheel of dairy industry. Surplus milk is generally converted into butter to be used during the times of scarcity. It is an article of commerce and a sign if wealth. Butter is a fat concentrate, which is obtained by churning cream, gathering the fat into a compact mass and then working it for proper distribution of moisture and salt. The art of butter making has a long history. The manufacture of butter was confined to colder regions where gravity creaming was successful. The milk was allowed to stand undisturbed for 10-12 hours until layer of cream collects at the top.

Cultural characteristics of bacteria deal with the appearance of the growth in various liquid and on solid media. The growth characteristics of most of the bacteria are typical of the group or species and help in their identification. The terminology employed to describe various features of bacterial growth are mentioned below.

Culture methods employed depend on the purpose for which they are intended. In the laboratory the indications for culture are mainly to

1.Isolate bacteria in pure culture.

2.Demonstrate their properties

3.Obtain sufficient growth for preparation of antigens and for other tests.

4.Type isolates by methods such as bacteriophages and bacteriocin susceptibility.

5.Determine sensitivity to antibiotics

6.Estimate viable counts

7.Maintain stock solution

Introduction

Advances in food preservation methods have supported the journey of food science from that of a cookery science to a fast growing industrial technology. Food preservation knowledge deals with the way to prevent spoilage and maintenance of quality in foods. Spoilage is the deterioration in quality brought about by inherent biochemical and chemical changes, as well as microbial metabolism during food storage. Basic principles of food preservation (Frazier et al., 2008) remains the same, but newer methods for achieving the goal is added through research fuelled by a consumer demand for fresh-like foods with convenience and safety. Scientific information on novel processing methods are beautifully compiled (Cullen et al., 2011; Da-Wen Sun, 2014; Tokuþoðlu and Swanson 2015; Doona, 2018).

In-depth knowledge of physiological activities of micro-organisms in presence of preservation agents is essential for preparation of safe foods with prolonged shelf life. Besides this, it is necessary to understand the effect of food constituents on microbial resistance against inactivation agents, to identify critical dose requirement. This profound knowledge would also help to understand kinetics of microbial inactivation and to develop mathematical models suitable for a realistic design of process parameters. For better appreciation of the following sections, readers are encouraged to have fundamental knowledge in microbiology and food processing. The sections in this chapter cover advanced microbiological knowledge pertaining to the preservation aspects of food.

1. Introduction

Milk and milk products constitute essential dietary components for a vast majority of the population. India with annual production of more than 135.6 million tonnes has emerged as leading milk producing nation. The per capita per day availability of milk in India is more than 290 g. Buffalo milk accounts for 57% of the total milk produced in India. Ethnic dairy products account for 90% of all dairy products consumed (Aneja et al., 2002). Many health benefits have been attributed to fermented dairy products and probiotic microorganisms (Salminen et al., 1998). The market for functional foods, nutraceuticals, wellness foods and beverages is fast growing. The harmful effects of synthetic drinks have propelled health conscious consumers to go for natural and traditional drinks. In search of better returns, the Indian dairy industry is widening its focus to include traditional milk products, and these are emerging as new profit centres for the organised sector.

Traditional dairy products have several advantages. They require low capital investment and give high profit margin. They are more appealing to the Indian palate. Dahi or curd is the most popular indigenous fermented dairy product. There are numerous references to dahi in vedic literature. The Prevention of Food Adulteration Act (Anon, 1976) describes dahi as a product obtained from pasteurized or boiled milk by souring (natural or otherwise), using harmless lactic acid or other bacterial cultures. Indian dahi and its western counterpart yoghurt differ considerably in terms of cultures used and their bouquet and flavour profiles (Aneja et al., 2002). The major flavor compound in dahi is diacetyl. Dahi made from buffalo milk has a firm body and white glossy appearance, with a cream layer on top. Mixed mesophilic cultures of Lactococcus lactis subsp. lactis, Lactococcus lactis subsp. cremoris, Lactococcus lactis subsp diacetylactis along with Leuconostoc species are grown together for production of dahi.

Dairy byproducts constitute an important segment of dairy industry. An important criterion in the economic choice of any process is the efficient utilization of byproduct that is produced along with the main product. The main byproducts of dairy industry are skim milk, buttermilk, whey and ghee residue. Each and every component of milk must be judiciously processed into edible form not only to improve the profitability of dairy industry but also to make best use of the unique nutritional value of the precious milk constituents on one hand, and to prevent environmental pollution, on the other. However, dairy plants, particularly in India, are usually confronted with the problem of byproduct utilization, especially that of whey and ghee residue in economical manner.

Skim milk is a byproduct obtained during the manufacture of cream. It is rich in solids-not-fat content and has high nutritional value. In dairy plants, it is mostly utilized either in standardization for the manufacture of main dairy products or preserved by removing moisture in milk powder form. The skim milk when utilized in either of these two forms or consumed as liquid, is not considered a byproduct. It is regarded as a byproduct only when it is either not economically utilized or utilized for derived byproducts, like casein and other related products, co-precipitates, protein hydrolyzates, etc.

Buttermilk is the byproduct obtained during butter manufacture. Sweet cream buttermilk resembles skim milk in gross chemical composition and is usually admixed with bulk of skim milk for further spray drying or even product manufacture in dairy plants. Desi buttermilk, on the other hand, has long been an important domestic beverage in India. It has high nutritive and therapeutic value. It also finds its way in the preparation of a host of culinary dishes such as kadhi, dhokla and idili. Also a number of federations and private plants sell salted and spiced buttermilk in 200 ml pouches.

30.1 Introduction
Every food processing facility, especially those that produce several products, generates trash in various forms. The food products are rich in carbs, proteins, lipids, and mineral salts. During the process of flushing and washing various equipment, nutrients are released into the drainage, promoting the growth of both anaerobic and aerobic bacteria. Consequently, the water that is flushed might have a highly unpleasant smell and could become a challenge for disposal in the municipal sewer system.

30.2 Definition of Waste Management
Waste management encompasses the collection, transportation, processing, recycling or disposal, and oversight of waste materials.

30.3 Types of Wastes in a Food Processing Plants
Waste produced in food processing plants can be categorized based on their form and origin, whether they are avoidable or unavoidable, their value, or their potential for reuse.

30.3.1 Based on the forms: Wastes based on forms can be classified as
1. Solid wastes,
2. Liquid wastes,
3. Oily wastes,
4. Gaseous wastes/water vapors.

Milk processing plants involve processing of raw milk into products like market milk, cheese, butter, ice cream, condensed milk, dried milks and traditional dairy products. The by-products processing includes buttermilk, whey and its derivatives. Dairy effluents contain dissolved sugar, protein, fat and minerals. Spills and leaks of products or by-products, residual milk or milk products in piping and equipment before cleaning, wash solutions from equipment and floors, condensate from evaporation processes and cheese trimmings are some of the sources of dairy effluents in a dairy plant. The key parameters are the biological oxygen demand (BOD), chemical oxygen demand (COD), total suspended solids, total dissolved solids etc. Common dairy waste techniques include grease traps, oil water separators for separation of floatable solids, equalization tanks. Biological treatment consists of aerobic and anaerobic process.

Dairy Industry in India
Milk holds a deeply symbolic place in Indian culture, far beyond its role as a mere food item. It is intricately woven into the cultural and historical identity of the nation, with the dairy industry serving as a cornerstone of India’s economy. The sector is a driving force behind rural development providing a crucial pathway to prosperity for millions of small-scale farmers. It plays a significant role in alleviating poverty and generating employment opportunities. The dairy sector by empowering farmers, developing modern infrastructure embracing technological advancements, and promoting sustainable practices, fostering widespread socio-economic progress in rural India. In the 1920s, modern milk processing and marketing technologies were introduced in India. The National Dairy Development Board (NDDB; founded in 1965), implemented Operation Flood (1970-96) to modernize the dairy sector through cooperatives. This led to the rise of dairy cooperatives countering the exploitation by private milk plants. The cooperatives followed the Anand model, a three-tier structure comprising village-level societies (producers), district-level unions (milk collection and processing), and state-level federations (marketing). Originating in Anand, Gujarat in 1946, the model was later adopted nationwide. The progress termed as White Revolution transformed India from a nation struggling with milk shortages into the world’s largest producer and consumer of milk. By 1997, India became the global leader in milk production with a volume of 70.9 million tons, surpassing the United States (FAO data rearranged by Our World in Data). Further in the year 2014, for the first time, it beat the entire European Union. Since 1950, India’s milk production has seen tremendous growth, rising from 17 million tonnes in 1950-51 to 230.58 million tonnes in 2022-23. The per capita availability of milk increased from 124 g/day in 1950-51 (PIB 2022) to 459 g/day in India during.

Introduction
 
Dairy ingredients are of significant importance in the formulation of many food products. Additionally, the incorporation of dairy ingredients, comprehensively recognized by the consumer as “natural” intensifies the odds of success of packaged foods and adds functional properties of the dairy ingredients in the targeted product. Dairy ingredients can be extracted from fluid milk in the form of cream, butter, condensed milk, dry milk, cheese, and whey products [1]. They provide beneficial functionality to foods, such as delivery of key nutrients, water management, fat-holding capacity, emulsification capability, viscosity creation, gel formation, and foam generation apart from providing desirable attributes of texture and flavour. Various ingredients and claims associated with dairy foods are shown in Table 3.1.

Milk is nutritious and essential food for human beings and also serve as good medium for microbial growth and contamination. Milk is a single, most nearly complete, natural food. Primarily it is meant for nourishing the newborns but man has exploited it for human consumption since long. Today milk has become indispensable integral component of our diet. About half the milk produced is consumed as fresh milk, the rest going into a variety of products including evaporated and condensed milk, dried milk powder, butter, cheese, malted milk, ice cream, yoghurt, lactose and casein. Milk is very important from a nutritional standpoint since it contained all the essential food constituents (carbohydrate-lactose, protein-casein, fat-butter, minerals- calcium, potassium and vitamins- A, B, C, D, E, and K) required in human diet in about the proper proportions. India is the second largest producer of milk in the world. India has exhibited unprecedented rate of growth in dairy development in last 30 years mainly due to sustained efforts of dairy technologists in Operation flood Programme introduced by the National Dairy Development Board. Dairy microbiology deals with aspects like shelf life and spoilage microbes, public health significance and food borne pathogens, and production of fermented dairy products.

DAIRY BUSINESS

Most ambitious young men at some time or another are likely to develop an interest in going into business for themselves. This is prompted not only by the thought that a person owning his own business is more independent but also by the hope of increasing personal income. However, when these same young men look about them and see the general existence of “big business” in practically all lines of endeavour, they often lose courage and decide to remain as workers with some company where they can enjoy a certain degree of security. 
The general condition is an important factor in determining the success of new business enterprises. Similarly, innovations like the creation of new products or the development of new methods of merchandising often gives rise to opportunities for a new business enterprise. The introduction of “Softy” ice cream and other soft-served frozen products made it possible for several hundred small businesses to get established in our country. The advent of single- service paper container was the spark that started several milk-plant operations which later grew into companies of considerable size. Ironically, the paper milk container also spelled the doom for many older independent plants that could not or did not adjust soon enough to this new way of packaging. The chocolate-coated ice-cream bar idea started a number of new enterprises which grew from small companies going to concerns of considerable size. Vending machines made it possible for many small companies to get started selling milk, ice cream, candy and lassi etc. 

Introduction

The dairy industry is a cornerstone of worldwide agricultural economies, underpinning nutrition, employment, and rural development. India stands out among nations with thriving dairy sectors, accounting for approximately 23% of global milk production (Prasad et al., 2023). With advent of Operation Flood in the late 1970s, India’s dairy industry has undergone transformative changes. This movement revolutionised milk production, rural income, and the organisation of cooperatives. The Indian dairy sector is still unorganized and only 18-20 per cent of the total milk produced is handled by organized sector (Gowda, 2023). There is rising trend of value addition along with rising demand of processed dairy products in India. These changes underscore the importance of effective dairy plant management systems, where strategic oversight of milk procurement, processing, and product manufacturing ensures quality, efficiency, and sustainability.

The GOI is pushing to strengthen infrastructure for the production of quality milk, as well as for procurement, processing, and marketing of milk and milk products through the following Dairy Development Schemes:

6.1. Dairy Entrepreneurship Development Scheme (DEDS)

Dairy Entrepreneurship Development Scheme (DEDS) was started in September 2010. This scheme is being implemented through NABARD which provides financial assistance to commercially bankable projects with loans from Commercial, Cooperative, Urban and Rural banks.

Objective of the scheme

To generate self-employment and provide infrastructure for the dairy sector, to set up modern dairy farms and infrastructure for the production of clean milk, to encourage heifer calf rearing for conservation and development of good breeding stock, to bring structural changes in the unorganized sector so that initial processing of milk can be taken up at the village level, to upgrade traditional technology to handle milk on a commercial scale, to provide value addition to milk through processing and production of milk products.

1.1. Global Dairy Scenario

Global milk production is forecast at 852 million tonnes in 2019, an increase of 1.4 per cent from 2018 (DAHD, 2019) – a smaller rate of growth, reflecting downward revisions made for India and the European Union. Much of the anticipated output expansion will originate in India, Pakistan, China, the European Union, and Brazil, partially offset by declines in some countries including Australia, Colombia, and Argentina. In India and Pakistan, herd expansions drive output growth, while in China, farm efficiency improvements underpin higher growth. The output is increasing in the European Union, although slowly as dry weather limited the supply of milk during the summer, whilst rising dairy herds and stable milk prices are supporting higher production in Brazil. By contrast, the output may decline in Australia and Colombia due to dry weather, whereas in Argentina, rising feed costs and restrained consumer demand may dampen production. Elsewhere, in the United States, higher milk yields hold up the growth momentum, whereas, in New Zealand, favourable weather corroborates a positive production outlook (DAHD,19).

    1.    The heifer is bred (naturally or by insemination) before the age of two years.

    2.    The gestation period is nine months and one week.

    3.    After calving, the cow gives milk for 10 months.

    4.    1 – 2 months after calving the cow will again be bred.

    5.    The cow then continues to give milk for about 300 days. This period is known as lactation.

    6.    Milk should preferably be handled in a closed system, to minimise the risk of contamination. It must be cooled to 4 °C as soon as it is produced.

    7.    The content of protein, lactose and ash is somewhat higher in buffalo milk than in cow milk. Buffalo milk contains vitamin A, but lacks b-carotene, which is present in cow’s milk.

    8.    When milk and cream turn to butter, there is a phase inversion from an oil-in-water emulsion to a water-in-oil emulsion.

    9.    In milk, the whey proteins are in colloidal solution and the casein in colloidal suspension.

Ice cream defects can result from formulation imbalance, poor ingredient quality, processing deviations, inadequate freezing or hardening, packaging problems, storage conditions, and cold-chain failures. This chapter provides a systematic framework for understanding, classifying, diagnosing, and correcting defects affecting flavour, body, texture, melting, colour, appearance, packaging, and storage stability. It emphasizes that a visible defect in the finished product may originate much earlier in the production or distribution chain. Examples include iciness caused by slow freezing or temperature abuse, weak body associated with formulation or overrun, and flavour problems originating from raw materials or microbial activity. Defect analysis therefore becomes an important tool for troubleshooting, quality improvement, training, and preventive action.

25.1 Introduction
Cream, butter, anhydrous milk fat, ghee and fat preserves, cheese, condensed milk, evaporated milk, skim milk, and whole milk powder are among the dairy products that have been extensively examined.
25.2 Defects in market cream
In the market, there are a variety of creams, including half cream, single cream, coffee cream, cultured cream, whipping cream, heavy whipping cream, aerosol cream, double cream, clotted cream, and high-fat creams (also referred to as plastic cream). The defects related to market cream are detailed in Annexure-I
1. Flavour defects in cream: Any flavor imperfections originally present in whole milk may become more noticeable in separated cream. Volatile compounds that are fat-soluble tend to concentrate in the fat portion, which can amplify these defects, such as an oxidized taste. Common flavor defects associated with cream include feed, cooked, malty, musty, utensil, weed, acid, bitter, and oxidized/rancid flavors. These flavor defects are further described in the section discussing flavor issues in Table butter.
2. Specific defects in cream
• Serum leakage, creaming, reduced overrun in whipping cream: Serum leakage typically results from partial churning (overwhipping), causing an unattractive liquid pool around the whipped cream or sogginess when applied to cakes. Excessive whipping leads to the formation of large fat globule clusters, which can break bubble-enclosing membranes, initiate bubble coalescence, and reduce overrun. This process may lead to an irreversible phase inversion, turning the cream into a greasy water-in-oil emulsion, visible as butter granules. Unfavorable creaming and serum separation can be delayed by lowering fat content (within legal limits), adding stabilizers like carrageenan, emulsifiers, or protein ingredients, or through post-UHT homogenization. Using a mix of kappa and iota carrageenan can help reduce creaming during storage and minimize whipped cream drainage.

1.1. General Discussion of Milk

“The cow is the foster mother of the human race. From the days of the ancient Hindoo to this time have the thoughts of men turned to this kindly and beneficent creature as one of the chief sustaining forces of human life”.

William Dempster Hoard (1836-1918)
Former Governor, State of Wisconsin, USA (1889-1891)
Founder of Hoard’s Dairyman (1885)

The oldest written records of the human race are in Sanskrit and are preserved in India. At the time these records began, about 6,000 years ago, milk had already become an important article of food. In fact, so important was the cow to these early people of South and Central Asia that wealth was measured in numbers of cattle, and the cow was in time made a sacred animal and is still so considered in India.

According to the best authorities, the domestication of cattle must have occurred somewhere between 6,000 and 10,000 years ago. The cow was worshipped in Babylonia, and in Egypt about 20,000 B.C. Over fifty references to cows and milk are found in Old Testament and the Promised Land was described as “a land flowing with milk and honey”.

Milk is the only food of the young mammal during the first period of its life. The substances in milk provide more energy and the building materials necessary for growth. Milk also contains antibodies which protect the young mammal against infection. A calf needs about 1000 litres of milk for growth, and that is the quantity which the primitive cow produces for each calf. There has been an enormous changes since man took the cow into his service. Selective breeding has resulted in dairy cows which yield more than 5,000 litres of milk per calf, i.e. five times as much as the primitive cow. Some cows can yield 10,000 litres or more.

INTRODUCTION

Drying or dehydration is defined as the removal of moisture from its initial level to a level which renders food materials safe for subsequent storage. It is a simultaneous heat and mass transfer operation in which heat is supplied externally to evaporate moisture and air is forced through the bed to carry away the moisture. Drying is the oldest technique to preserve food materials. Advantages of drying include reduction in water activity, enhancement of shelf life, maintenance of flavor and nutritive value, and reduction in packaging and transportation costs. When the water activity of the dried product is reduced to below 0.6, most microorganisms do not grow and the rates of other deteriorative reactions are substantially reduced.

Introduction

Flowering plants (Angiosperms) are identified by their morphological characteristics like flower, fruit and seeds that help their taxonomic characterization into different species, genera, families and orders. The floral characters which are used for taxonomic characterization of plants include the type of inflorescences, perianth structure, floral symmetry, arrangement of floral leaves, type of androecium, stamens, gynoecium, carpel, ovule, etc. The type of fruit and seed also play important roles in taxonomic classification. In this chapter we will briefly discuss about various morphological features of flowering plants (in botanical terms) that are useful in taxonomy.

Nomenclature of Plant

Nomenclature of plant is the formal and scientific naming of plants. It is related to but distinct from taxonomy for grouping and classifying plants and provide botanical name.

The scientific/botanical nomenclature started with Species Plantarum by Carolus Linnaeus in 1753. Botanical nomenclature is governed by the International Code of Nomenclature for algae, fungi, and plants (ICN), which replaces the International Code of Botanical Nomenclature (ICBN).

ICBN and ICN

International Code of Botanical Nomenclature, (ICBN) was adopted by the VIIth International Botanical Congress held in Stockholm, July1950. While the International Code of Nomenclature for algae, fungi, and plants (ICN) was adopted by the XVIIIth International Botanical Congress held in Melbourne, Australia in July 2011.

Abstract

Designer foods are known by various names like medical foods, fortified foods, nutritional foods, nutraceutical foods, pharma foods etc. These foods are nutritious, maintain health and prevent diseases. Such foods must possess characteristics like modified composition; limit the presence of certain potentially harmful components and include certain desirable ingredients either naturally or by intentional addition. The term designer food emerged some years ago to describe foods tailored to specific concentration and proportion of nutrients that are important to health. These are designed and produced by using different approaches. The term ‘designer foods’ is subjected to change based on nutritional labelling regulations, new developments, consumer perceptions and expectations. Grain improvement through biotechnology emphasizes crop upgrading with a specific challenge to manipulate the endosperm composition in order to improve its intrinsic properties for diet and health. Breeding fruits with higher bioactive compounds for disease resistance and improved composition have stimulated interest in the nutraceutical and processing aspects of the fruits Nutritive and genetic interventions to alter milk composition for specific health and processing opportunities are gaining importance in dairy biotechnology. Designer eggs having modified fat provide choices with different nutritional benefits. The options suggested for designer meat and meat products are through production practices, post-harvest procedures and reformulation techniques. All these features of designer foods will be presented with suitable examples.

1 Detection of Starch Introduction

Starch is widely available in several forms such as wheat flour, corn flour and commercially manufactured starch. It is extensively used by adulterators to raise the SNF content in the milk. When high amounts of starch are added to milk, it can cause diarrhea due to the indigestion of starch in colon. Apart from this, accumulation of starch in the body may prove very fatal for diabetic patients. In this method, starch in milk can be detected by using iodine solution. Iodine from the iodine solution gets absorbed on the starch molecule surface and resultant product of adsorption has deep blue colour.

Reagents

 • Potassium iodide

 • Iodine solution - 1% (Add 1 g of iodine and 5 g of potassium iodide in

distilled water and dilute to 100 ml)

Wood apple (Feronialimonia) commonly known as Bael in various Indian sub-continent languages is the most under-utilised fruit despite of its aromatic flavor, high nutritional components and important medicinal values. Reason may be due to challenge in harnessing the pulp from the fruit. It is considered under seasonal fruit but it has long duration of shelf-life as compared to other fruits.The physic-chemical characteristics revealed that it has high shell weight of 55.67% as compared to pulp weight of 43.77% (Devi et al. 2018).

A. Estimation of Calcium

Requirements : Pottasium permanganate solution (0.1N) – Dissolve by heating 33g of potassium permanganate in 1000ml distilled water and allow to stand in coloured bottle for several days. Filter contents through 6mm thick film of asbestos mat on Gooch crucible.

Asbestos mat – Digest asbestos with dilute hydrochloric acid(1:3) for 2-3 days, wash free from acid and digest with 10% sodium hydroxide for few days, wash free from alkali. Digest with dilute Nitric acid(1:3) for several hours and wash. Shake with water into fine pulp and digest with 0.1N potassium permanganate solution that has been acidified with dilute sulphuric acid (1:3), for 1 hour and wash. Use this pulp for layering 6mm film on Gooch crucible.

Dilute hydrochloric acid (1:4), Ammonium hydroxide solution (1:1), Saturated ammonium oxalate solution, Diluted sulphuric acid (1:4)

Developing a nutraceutical or functional food is becoming mandatory as looking at the current nutritional status of the population over the world. Nutraceutical foods are proven to cause several health benefits to people. Hence the basic aim behind developing the nutraceutical chocolate bar was to fulfill the nutritional demands and provide some additional benefits to people. The product mainly contains two key ingredients that are, Drumstick (Morings oleifera) which has the most qualities to call a Superfood” and Quinoa (Chenopodium quinoa) which is referred to as “Mother of all grains”.

Introduction

Meaning of Entrepreneur

The term “entrepreneur” is derived from the French verb entreprendre, which means “to undertake.” Historically, its meaning has evolved. In the early 16th century, it referred to individuals involved in engineering tasks like construction. By the 18th century, the term came to describe those engaged in economic activities. Over time, the concept of an entrepreneur shifted from someone participating in military ventures to someone actively involved in business and economic pursuits. Today, an entrepreneur is defined as an individual who innovates, organizes resources, and takes on risks to create new ventures or products with the goal of generating profits.

Richard Cantillon was the first who proposed the term “entrepreneur is an agent who buys factors of production at certain prices in order to combine them into a product with a view to selling it at uncertain prices in future.”

The proposal presented takes into account the rationale of using natural source of immunity, discarding any preservatives or artificial flavors and additives. Collection of fresh produce followed by processing of fruits and vegetables, in the form of a veggie mix slushy which is ready to eat, ready to cook and easy to digest, through the use of sustainable retorting technology. The proposal takes into account the major problems regarding food for the elder like lack of digestion, in sufficient appetite, lack of value in terms of vitamins and minerals of food, also targeting the notion of feeling sick by taking multi vitamin and immunity tablets on a regular basis.

Introduction

A marketing plan is a strategic document that guides an organization in generating leads and engaging with target customers. It serves as a roadmap for how a company will approach the market, akin to a job description for the business. Every organization needs a well-crafted marketing plan that is tailored to its specific objectives, regularly updated, and aligned with customer demands. Companies with a solid marketing plan tend to be more successful and outperform their competitors compared to those without one.

The marketing plan outlines a strategy for growth by detailing how a company will attract and retain customers. It is an integral part of the overall business plan and lays the foundation for future market opportunities. A strong marketing plan focuses on customer needs and provides a clear roadmap for launching new products, expanding the customer base, and boosting the company’s profile. It includes essential elements such as business goals, mission, budget, and strategies. A well-designed marketing plan ensures effective promotion of products and services, helping businesses reach their target market and track success. Moreover, it should be flexible and adaptable to the evolving market landscape.

Introduction

Oils and fats are utilized both for their food applications as well as for industrial purpose. As a food product, oils and fats are consumed in a variety of forms such as shortening, ghee, butter, margarine, cooking oil, salad oil, etc. Other uses include their usage in the form of lubricants, greases, soaps, cosmetics, paints as well as animal feed and biofuels. Among the Asian countries, Indonesia leads as the prime producer with more than fifty percent of the total palm oil production in the world. As a second-best producer of oils and fats, China was the major manufacturer of rapeseed as well as soybean oil in year of 2018. In general, Asian countries contributed to more than fifty percent of the total fat and oil manufacturing in the world in 2018 (Kushairi et al., 2018). Even though fats and oils are primarily utilized for food applications, there has been an increased interest in their industrial utility for the manufacture of biofuels. Fats manufactured from various animal sources like tallow or lard also contribute to a considerable quantity and compete with vegetable oils for the production of biofuels.

The resources for producing oils and fats are diverse depending on their end use, yet they are obtained from two major sources: animal sources and plant sources. Oils and fats are used as starting materials for the preparation of shortenings, margarines, and other tailored products that further act as functional ingredients in food products prepared by food processors, restaurants, and in home. They have been used for food and other variety of applications since the prehistoric times, due to their easy availability. For example, fatty tissues from animals liberate free-floating fats on being boiled, whereas oil can be pressed out from oil fruits and oilseeds.

The role of dietary oils and fats in human nutrition is also one of the important areas of interest. Over the time, oil and fat industry has provided significant contributions toward nutritional improvement of various products. With the growing concern linked between cardiovascular diseases and consumption of oil and fat products, low-calorie variants of different foods, free or low in cholesterol are also available now. Excessive fat consumption among the populations of developed countries and consequences of obesity has led to the development of reduced-fat and very-low-fat variants of foods.

Abstract

The concept of functional foods has received significant media attention and, as a result, interest has focused on the need for research into the non- nutrient constituents of food plants. This has enabled market penetration of functional foods containing increased amounts of bioactive food compounds or physiologically active nutrients and non-nutrients within food that impart proven health benefits. Functional foods can take many forms. Some may be conventional foods with bioactive components that can now be identified and linked to positive health outcomes. Some may be fortified or enhanced foods, specifically created to reduce disease risk for a certain group of people. Numerous observational studies have shown that omega-3 fatty acids enriched diets are associated with a reduction of cardiovascular mortality, heart attack and sudden death. In several studies, a higher fish intake has been associated with decreased incidence of coronary artery disease (CAD) and cardiovascular mortality. A trial revealed that fish oil supplementation of 130 mg/kg of body weight each day decreased the number of stiff joints, duration of morning stiffness, pain and global arthritis activity. Many supplements contain active ingredients that have strong biological effects in the body. The bioavailability of nutrients within a given food is governed by the composition and microstructure of the food, commonly referred to as the food matrix. More specifically, numerous studies investigating the development of functional foods have shown that the interactions between nutrients and non-nutrients within the food matrix can be synergistic, additive, or neutralizing in nature. The preventive and curative effects of the functional foods on health concludes it to be integral components of established public health programs to reduce the risk of specific diseases.