eChapters

Low-temperature preservation is one of the most widely used physical approaches for extending food shelf life without seriously affecting quality. Reducing storage temperature slows microbial multiplication, enzyme activity and chemical deterioration, with inhibition increasing as temperature declines. Refrigeration maintains food above its freezing point, while freezing reduces temperature below zero and converts available water into ice. The chapter examines conventional refrigeration, advanced chilling, freezing, cryopreservation, superchilling and the influence of ice crystallisation on food quality. It explains how temperature, cooling rate and crystal size affect texture, nutritional retention and storage stability, while also addressing the limitations and quality changes associated with prolonged low-temperature storage.

Thermal preservation is among the most established methods for improving the safety and storage life of food. It uses controlled heating to destroy spoilage organisms and pathogens and to inactivate undesirable enzymes. Processing intensity varies according to the target microorganisms, food composition, packaging and required shelf life. Pasteurisation reduces pathogenic and spoilage microorganisms without achieving complete sterility, while sterilisation destroys more heat-resistant organisms and spores to produce commercially stable foods. Ultra-high-temperature processing combines very high temperatures with extremely short treatment periods. This chapter also discusses retort processing, thermal-death kinetics and microbial-inactivation models used to design safe processes while minimising nutritional and sensory damage.

Several centuries ago, perhaps as early as 6000-8000 BC, ancient man learned to domesticate species of animals for obtaining milk for their consumption. These included cows, buffaloes, sheep, goats, and camels, all of which are still used in various parts of the world for the production of milk for human use. The significance of milk as the most wholesome single food available in nature has never been debated. It is as ancient as mankind itself. The role and consumption pattern of milk in the traditional diet varies widely in different regions of the world. The tropical countries have not been traditional milk consumers, whereas in Europe (especially Scandinavia) and North America, milk and milk products have been traditional parts of the diet.

Galactan : It is a polysaccharide in which major monosaccharide subunit is galactose and often found among the pectic substances of cell walls.

Galactoblast : It is also called colostrums. It is female’s first milk after parturition.

Galactometer : It is an instrument used for recording the specific gravity of the milk.

Galactorrhea : A spontaneous flow of milk from the nipple is called galactorrhea.

Introduction

The emerging functional foods market presents a considerable challenge to all manufacturers to keep abreast of the use of adequate packaging materials for their products. The interest in functional foods is intrinsically linked to the potential health benefits of their bioactive components. Preserving these bioactive components is, therefore, a crucial factor to providing the anticipated health benefits of functional foods. However, recurring failure in maintaining the quality of functional foods has been reported. Functional foods include a wide variety of products such as prebiotics and probiotics, dried foods (fibers, tea, herbs, etc.), fermented products (yogurts, kefirs, vegetables), and fresh fruits and vegetables. Their bioactive components (minerals, vitamins, fibers, peptides, proteins, w–3 polyunsaturated fats, antioxidants, enzymes, and symbiotics) range from very simple molecules to complex living organisms such as bacteria.

Introduction

The search for weight controlling foods began in the mid 19^th century when industrialization led to widespread obesity. Over the years, people have tried to lose weight by avoiding particular food groups like carbohydrates, eating specific foods such as cabbage soup, or adding a certain ingredient, like vinegar or grapefruit to each meal. Recently scientists have demonstrated that green tea, dairy products, and cinnamon have the potential to improve weight control by altering fat and/or carbohydrate metabolism at the sub-cellular level. Considerable research is needed, however, before these food items can be used to develop effective weight management products.

Definition

Packaging is the science, art, and technology of enclosing or protecting products for distribution, storage, sale, and use. Packaging also refers to the process of design, evaluation, and production of packages. Food packaging is packaging for food. It requires protection, tampering resistance, and special physical, chemical, or biological needs. It also shows the product that is labeled to show any nutrition information on the food being consumed

Packaging may be defined as co-ordinated system of preparing foods for transport, distribution, storage, retailing and their use. It is also a means of ensuring safe delivery of goods to the consumer in sound condition without affecting adversely the quality of the product packed in it at a reasonable cost. Packaging thus has as “techno-economic function” aimed at maintaining the quality of food stuff packed, with a view to retain the quality for a reasonable period.

Packaging plays a vital and dominant role in marketing, in maintaining quality and in preserving the food.

Consequent to the increase in the consumer awareness of the healthy hazards, which may be posed by different types of packaging material. It becomes inevitable for the packers to use better quality, hygienic packaging material which could retain the quality of the product for a longer time without posing any health hazard. The choice of a suitable packaging becomes all the more necessary for a number of factors like physico-chemical characters and acceptability of the commodities packed in any packaging material. Apart from this the package should be of the right shape and size and must attract the consumer's eye which could offer an absolute confidence regarding the wholesomeness of the products pack in it.

When natural constituents of whole milk are altered by addition, removal, exchange and / or treatment, the resultant milk is designated as special milk. Recent years have witnessed a large increase in market penetration of special milks into the total fluid milk market. In many countries, now a large portion of fluid milk is consumed in the form of special milks. Manufacturers of special milks offer numerous advantages that are broadly related to dietary, cost and scarcity aspects. Special milks provide an avenue to diversify the milk with variety of flavours and other characteristics.

Milk is considered as nature’s almost perfect food. However, milk in its natural form is not considered suitable for many more healthy people who do not like the taste of milk as such. All these problems can be overcome by manufacturing special milks such as dietetic milks, low fat milks, flavoured milks, etc.

Butterfat is the most expensive constituent of milk. Therefore, the cost of buffalo whole milk is very high and many people particularly the weaker section of our society cannot afford to buy it. Consequently, manufacture of low priced toned milk was introduced in many markets. This also enabled the dairy plants to meet the ever increasing demand of milk, particularly during lean season. Replacement of butterfat by vegetable fat could also help in reducing the cost of milk

Due to seasonal fluctuation in milk production, there is acute shortage of milk during the lean period in our country. In certain parts of the world fresh milk is not produced at all. Manufacture of reconstituted and recombined milk from anhydrous milk solids can overcome such whole milk shortage. These anhydrous milk solids could either be prepared from the domestic seasonal surplus or marketed from those countries that have surplus milk production. The machinery and manpower of a market milk plant can be fully utilized all the year round by such diversifications.

Traditionally, Labour Laws have a protective function consisting of established standards both to protect workers in their workplace and to provide them a basic minimum level of living conditions. Alongwith this function, there also existed in most systems, a legal framework for the regulation of industrial relations between employers and workers. These functions had their impact on problems of development. The emphasis was more on labour legislation for its own sake, development being a separate proposition. This mutual lack of concern was perhaps due to historical factors. The most important aspect is the fact that in developed countries of the Western world, labour legislation followed the emergence of industrialization and in response to a demand for economic and social betterment of the workers, whereas in developing countries the emergence of labour laws preceded industrial growth.

Milk is nutritious and essential food for human beings and also serve as good medium for microbial growth and contamination. Milk is a single, most nearly complete, natural food. Primarily it is meant for nourishing the newborns but man has exploited it for human consumption since long. Today milk has become indispensable integral component of our diet. About half the milk produced is consumed as fresh milk, the rest going into a variety of products including evaporated and condensed milk, dried milk powder, butter, cheese, malted milk, ice cream, yoghurt, lactose and casein. Milk is very important from a nutritional standpoint since it contained all the essential food constituents (carbohydrate-lactose, protein-casein, fat-butter, minerals- calcium, potassium and vitamins- A, B, C, D, E, and K) required in human diet in about the proper proportions. India is the second largest producer of milk in the world. India has exhibited unprecedented rate of growth in dairy development in last 30 years mainly due to sustained efforts of dairy technologists in Operation flood Programme introduced by the National Dairy Development Board. Dairy microbiology deals with aspects like shelf life and spoilage microbes, public health significance and food borne pathogens, and production of fermented dairy products.

SINGLE CELL PROTEIN (SCP) AND PROTEIN DEFICIENCY

Protein is an essential component of diet. The greatest single problem in the world today is global food protein shortage. Having adequate quantity of food is only one side of the picture, but the most important aspect is the supply of balanced and nutritious food. All efforts are being made to increase the food production through modern methods of farming. But very little attention is being paid towards protein requirements and production. Approximately half of world population suffers from the protein deficiency disease called ‘Kwashiorkor’ and children with poor protein are victims of this disease.

Usually, cereals have low protein values while protein rich pulses are not available in desired quantities due to poor yields and high costs. The good quality protein available in fish, meat, poultry, and eggs is not available due to poor economic condition as well as varied dietary habits. With the current system of production, agriculture cannot be relied upon to feed an ever increasing world population. Hence there is an urgent need to find other protein sources. The best potential is seen in microbial protein or single cell protein (SCP), a new source of protein independent of agriculture.

DEFINITION: The dried cells of microorganisms such as bacteria, fungi, yeasts, algae that are grown in large scale culture systems as proteins for human or animal consumption are collectively known as "single cell proteins".

Ex.No.1. MICROBIAL SPOILAGE OF FRUITS

Fruits are living structures that continue to perform metabolic reactions and sustain physiological processes for a considerable length of time after harvest. The post-harvest infection of fruits by microorganisms may cause serious losses of fruits both in terms of quantity and quality. Initial infection which may be the direct result of mechanical or insect damage is followed by invasion of fruit tissues by secondary infection by common saprophytic organisms. . Many bacteria and fungi, for instance, are involved in decay after harvest. Typical fungi include Alternaria, Botrytis, Colletotrichum, Fusarium, Monilia, Penicillium, and Rhizopus. These fungi are generally weak pathogens, in that they usually infest only weak or damaged fruit. Molds are the most predominant spoilage organisms in fruits. Fungal rots in fruits like apple show brown or cream coloured areas.

Bacterial soft rot caused by Erwinia carotovora is wide spread in fruits that are not very acid. Fruits like straw berries, melons are in direct contact with moist soil and become infected from that source.

One of the major discoveries that gave birth to the field of microbiology was the development of instruments that could magnify, so that objects too small to be seen with the naked eye could be visualized. Robert Hooke in 1665, using an instrument with two sets of lenses (a crude compound microscope), was the first person to describe cells as life’s smallest structural units. In the early 1670s Anton van Leeuwenhoek used a simple magnifying lens to observe microbes he called ‘animalcules’ in rainwater, and was possibly the first person to observe microorganisms. The development of electron microscopes in the 1930s enabled magnifications of a further hundred times greater to be achieved.

A microscope can be defined as an optical instrument consisting of a lens or a combination of lenses for getting magnified images of minute objects. A simple microscope is essentially an ordinary magnifying lens held on an adjustable stand. It can give magnification up to 20 times the size of the object.

Improvements in microscopes enabled scientists to study the ‘invisible’ organisms – that is, those too small to be seen with the naked eye. They were able to show that certain of these microscopic creatures are responsible for the infectious diseases of higher organisms. Most of them are harmless, some are essential for life and some are used in the manufacture of products that are of benefit to humans.

13.1 Introduction

Hospitality industry is the second largest industry in the world. It is well diversified and can be chosen as per the interest. Hospitality management involves planning, organizing, directing and controlling human and other resources within the different segments of hospitality industry i.e. lodging, restaurant, travel and tourism, institutional management, recreational management, meeting and convention planning industries. Although, these all are separate but related segments of the hospitality industry and are interrelated to provide good and efficient services to the guests and users. Hospitality industry provides services which are need oriented and differ in various parts of the world as per regions and the traditional styles.

The first building created specifically for hotel was ‘City Hotel’ with 73 rooms at 115 Broadway, New York in 1794. Pallonjee Pestonjee established the very first western-style hotel in India, “The Bristol Hotel” in Mumbai in 1840. According to the report of the department of Tourism, Government of India, at the end of the year 2004, there were 167 Five star hotels with approx. 28,900 rooms, whereas 126 Four star hotels, with 8,831 rooms [2]. To expedite the ongoing process of classification/re-classification of functioning hotels in one to three star categories, five Regional Committees have also been set up in Delhi, Mumbai, Kolkata, Guwahati and Chennai to conduct inspection [3].

Abstract

Consumer now-a-days relies on manufacturing and processing standards, particularly to know what ingredients are present, due to dietary, nutritional requirements, or medical conditions (e.g., diabetes orallergies). Modified PCR and combination of PCR with other analytical tools have been successfully used for analysis of various parameters related to functional foods such as detection and quantification of beneficial as well as food borne pathogenic microorganisms, food allergens, raw material source, bioactive compounds, genetically modified foods. Multiplex PCR method has been found to be an efficient tool for simple, rapid, and reliable identification of Lactobacillus and Bifidobacterium in probiotic products. Food allergens like gluten, peanut, hazelnut etc. could be easily detected in foods using PCR, even if present in small quantities. BOX-PCR and PCR-DGGE analysis have been used for identification of bacterial diversity of a naturally fermented functional food (Enzyme). HPLC combined with real time -PCR were successfully used to quantify and analyse compounds of secondary metabolites like phenylpropanoid amides, which represent an important class of nutraceutical having strong antioxidant and chemotherapeutic effects in various transgenic tomato tissues. Detection of active compounds in natural plant products were successfully achieved using PCR techniques. In general PCR have gained more importance as it has high sensitivity, improved precision, wide dynamic range for quantification, genotype based and is quick method of detection and quality control of foods than traditional culture based methods.

Abstract

Prebiotics are the non-digestible food ingredients that beneficially affect the host by selectively stimulating the growth and/or activity of one or limited number of bacteria in the colon. These are heat resistant components which remain intact during the baking process; this allows prebiotics to be incorporated into every day food choices. Consumption of non-digestible ingredients allows the growth ofbio cultures by reaching the intestine unaffected by the digestion process resulting in improvement of digestive health. Prebiotics reach the intestine in an unaltered form and shows the prebiotic effect when there is an increase in the activity of healthy bacteria in the human intestine. The prebiotics stimulate the growth of healthy bacteria such as bifidobacteria and lactobacilli in the gut and increase resistance to invading pathogens. Functional foods increase consumer choice by adding prebiotics to every day food items such as cereals, biscuits, breads, table spreads, drinks, and yoghurts. By continuing to eat and drink common foods, but choosing functional alternatives (such as bread containing prebiotics) dietary requirements can be met, without significant changes to foodpreferences. Some common example of cereal based products containing prebiotics are oat-based prebiotic drink, Breakstone's and Knudsen live active cottage cheese, prebiotic yogurt- Attune and Muller vitality, multi-fruit drink enrich and flavoured water enrich, prebiotic flavoured water, prebiotic capsules, dried fruit bars, prebiotic powder- Biocare fructo-oligosaccharides, Newtree purplaisir chocolate spread. The claims are constipation relief, suppression of diarrhea and reduction of the risks of osteoporosis, atherosclerotic cardiovascular disease associated with dyslipidemia and insulin resistance, obesity and possibly Type-2 diabetes. At present, claims about reduction of disease risk are only tentative and further research is needed.

Abstract

The use of biosensors is growing in the food industry as a means to ensure food safety and quality because of its versatility in determining biological compounds. It is defined as analytical technique used for detection of analyte that combines a biological component with a physico-chemical detector. The most important contribution of biosensors in the food sectors is to control production processes and ensure food quality and safety by reliable, fast and cost effective procedures. Biosensors have been useful for the evaluation of food composition, particularly in functional food products fortified with phytochemicals, vitamins and minerals.. Biosensors are replacing conventional analytical tools since they offer advantages in size, cost, specificity, rapid response, precision and sensitivity. Future progress in the biosensor technology involves the development of nutraceutical release technology within packaging material where it can be used to trigger the controllable release of bioactive components and targeted delivery of protective agents into the food product. The growing consumer demands for safe and quality food has led the food industries to search for quick and reliable methods for which the applications of the biosensor technique in the field of food processing and quality control are found to be promising.

Abstract

Chitin has been ranked at second position after cellulose as the most abundant natural biopolymer. Chitin has similar chemical structure to that of cellulose with 2-acetamido-2-deoxy-b-d-glucose (NAG) monomers attached via ß (1–>4) linkages. Chitosan is the deacetylated (to varying degrees) form of chitin, which, unlike chitin, is soluble in acidic solutions. Therefore, Chitosan, a copolymer of glucosamine and N-acetyl glucosamine, is amongst the most copious biopolymers on earth and finds wide range of biomedical and industrial applications. It has been approved in 1983 by USDA as a feed additive. Chitin basically is obtained from prawn/crab shells and chemical treatment of chitin produces chitosan. Since supplies of seafood waste are seasonable and variable, so fungi can be used as alternative source for chitosan. The unique physicochemical properties of chitosan provide great potential of its utilization in food industry especially dairy industry such as production of dairy products, dairy waste water treatment, extension of shelf life of dairy products etc. In recent years, exotic synthetic compounds are losing their appeal, thus application of chitinous products in dairy as well as processing aids has received considerable attention. The modified chitosan with greater functional properties has increased application in different dairy foods. This chapter focuses on the fundamental uses of chitosan and their derivatives in a variety of dairy industry applications.

Abstract

Consumers increased response to new products with high sensory, organoleptic and nutritional potentials has impelled a search for new substitutes to processing methods. For several years, thermal processing was the main technology for producing safe products with long shelf lives, although in most cases losses of fresh flavors, vitamins, and some physicochemical characteristics were the price of safety and long-term stability. Pulsed electric field (PEF) processing as a non- thermal technique has been proven to inactivate microorganisms with minimal losses of flavor and food quality, possibly making it the answer to present-day consumer demands. In addition, the low processing temperatures used in this non- thermal technology allow the process to be nutrient preserving, energy efficient, which translates into lower costs and fewer environmental impacts. In this chapter, the the configuration of various PEF equipment, their electric configuration and recent application of this technique in processing of foods have been discussed in detail.

4.0. Introduction

Quality is a measure of the degree of excellence or degree of acceptability by the consumer. By quality, one can differentiate the individual unit of a product from the other units and can determined the degree of acceptability of the individual unit by the consumer. The quality may also be defined in term of end use and may vary depending upon consumer’s perception and need. According to USDA Marketing Workshop Report (1951) “quality is the combination of attributes or characteristics of a product that have significance in determining the degree of acceptability of the product to the user”. For industry, quality is a measure of purity, strength, flavour, colour, size, maturity, workmanship and conditions or any other distinctive attributes of the product.

According to Code of Federal Regulations “Quality is the inherent properties of any processed product which determine the relative degree of excellence of such product and includes the effect of preparation and processing and may or may not include the effects of packaging or added ingredients/ additives”.

Introduction

Food safety and quality assumes paramount significance in any food industry. Globalization has driven both the food and agricultural firms to adopt systems that functions in a way to interlink food standards and international trade so as to achieve superior food quality and safety. Food industries follow several regional and international food safety procedures to procure certification from the government and other agencies in order to survive in both domestic and international markets (Ryan, 2014). The essential components in the food safety include handling, preparation and storing of the food products to ensure the food quality and to avoid food-borne diseases. The unwanted substances in a food product included by an accident is known as contaminants and the unwanted substance added to the food product to reduce the cost or to improve the other properties are known as adulterants. Contaminants and adulterants may lead to pathogens and toxins that pose serious concerns over the health of the consumer (Jha, 2016). The adulterants and contaminants are classified as physical adulterants and contaminants, chemical adulterants and residues, microbiological and pathogenic contaminants and toxins.

Hazards in food can be categorized into physical, chemical and biological.

Physical Hazards: Physical hazards are foreign organic or inorganic objects or extraneous materials in the food products. Hard and sharp physical hazards are of particular concern. Examples of physical hazards are broken glass, plastics, metal pieces, wood pieces, stones etc.

Introduction

Thermal processing is an important treatment for food preservation is, especially in the manufacture of shelf-stable foods with specific nutritional properties. It is also indispensable for determining the sensory properties, particularly, colour, flavour, and texture of fried, baked, and roasted products. As a result of heat treatment, chemical changes induced, and a plethora of new molecules are formed in foods, some of which have been claimed to be health-promoting antioxidants and anti-mutagens. However, reports suggest that thermal processing leads to the formation of certain toxic compounds, so-called process-induced contaminants in foods. The process-induced toxicants are certain compounds which are formed in food as a result of food processing, exert adverse toxicological effects, and cause potential health risks to humans. Acrylamide, furan, chloropropanols, heterocyclic aromatic amines, polycyclic aromatic hydrocarbons, and N-nitrosamines are some well-known examples of thermal process contaminants.

Over the past several years, there has been significant change in consumer perception on quality and food safety. Consumers are demanding healthy, nutritious and cost-effective products processed under controlled hygienic conditions. Food safety concern, in recent years, has become dominant and important. The requirement of food safety and quality assurance has never been so demanding as it is now. Globally food safety is being addressed independent of food quality. In anticipation of consumer demands on food safety, food sanitary standards at national and international levels are under constant review. Several inter governmental agencies / organizations are engaged in work related to quality assurance and food safety. Amongst these World Health Organization (WHO) and Codex Alimentarius Commission (CAC) play the major role in protecting public health and defining standards of quality and safety aspects of foods for regional and or world wide usage.

Regulatory Institutions

The quality environment in the Indian food processing sector revolves around compulsory legislation which specify minimum standard and certification system. Legal standards are generally made to have control over the quality of foods offered for sale and to safeguard the consumer from health hazards posed by practice of adulteration. There are basically three types of Indian standards namely

Introduction

Biotechnology is defined as the ‘application of scientific and engineering principles to the processing of material by biological agents to provide goods and services’. The term ‘biotechnology’ is also used when a technique involving live organisms are employed to make or modify a product, improve plants or animals or develop microorganisms for specific uses. According to the definition of the Codex Alimentarius Commission (CAC 2001), modern biotechnology is defined as the application of (i) in vitro nucleic acid techniques, including recombinant deoxyribonucleic acid (DNA) and direct injection of nucleic acid into cells or organelles, or (ii) fusion of cells beyond the taxonomic family, that overcome natural physiological reproductive or recombination barriers, and that are not techniques used in traditional breeding and selection. Scientific developments of present world owe to processes and products of the field of biotechnology. Modern biotechnological advancements have played a major role in important branches of science such as agriculture, food processing, medicine and veterinary applications. Biotechnological interventions of the downstream processing applications include primary production areas namely breeding of crops and livestock, production of additives for food and feed and pharmaceuticals along with diagnostic tools. Inputs for food processing applications from biotechnology include biocatalysts such as enzymes, tracing food ingredients such as Genetically modified (GM) ingredients in end products of food and feed and food quality testing applications for food borne pathogens. Applications of modern biotechnology in form of genetically modified organisms have gained much importance in comparison to the downstream applications during food processing and food safety (Zhu, 2017). Food processing operations include transformation of harvested crop or animal produce into produce which are marketable with long shelf life and minimises waste and losses of raw produce in food chain and ensure their continuous availability in super market shelves. Food safety is an important area which assures of the food being safe for consumption. Food technological applications of modern biotechnology includes food enzymes that are important in various food processing applications in reactions involving lipids, carbohydrates and proteins, food fermentations to improve properties such as taste, flavour, texture, nutritional and shelf stability, food additives in form of bio preservatives, flavours, fragrances, genetically modified starter cultures, genetically modified crops, modern rapid diagnostic kits for food safety are discussed in detail.

Introduction

The improvement of the quality of foods can be made by fortification of foods nutrients quality and quantity in food. It can be a very cost effective public health intervention. As a result of the high consumption pattern of dairy products viz. yogurt, the fortification process will effectively diminish or prevent diseases associated with nutritional deficiencies (Gahruie et al., 2015). However, due to nutrients deficiencies in human population especially in certain stages of life, holding, importing, and consumption of fortified foods is increasing (Preedy et al., 2013). As a general rule, addition of one or more essential nutrients to a food and increasing their concentration in that particular food to levels higher than normal is known as fortification. This is intended at preventing and correcting shortages in one or more micronutrients in the society or specific population groups (Bonner et al., 1999). The major ways of food fortification are (1) commercial and industrial fortification for wheat flour, corn meal, cooking oils, (2) bio-fortification of crops to augment their nutritional value, which can include conventional as well as selective breeding, and genetic engineering and (3) house hold fortification like vitamin D drops. Fermentation has twofold outcome on glycemic index though there are conflicting results after consumption. The reduced glycemic index of fermented food has been featured to the production of short?chain organic acids during fermentation.

Introduction

Food ingredients sector is indicating ample prospects for growth. The segment is an obligatory element for the growth of the food and beverage sector. These ingredients are also driving the development of the healthy functional foods industry to increase by 20% growth annually. Innovation in the food and beverage market, especially in the processed food domain, has created innumerable opportunities for new product development in the ingredients market as well. The segment is driven by the growing food processing industry and India is emerging as ingredients bowl of the future. Additionally, growing urbanization coupled with industrialization and changing lifestyles have led to growing demand for convenient food that offers health benefits, over and above the basic nutritional benefits of the foodstuff. There are nearly 10 to 15 broad classes of ingredients that include the very basic ingredients as well as certain value added ingredients that have niche applications. Key ingredients are preservatives, flavours, colours, hydrocolloids/stabilizers, emulsifiers, artificial sweeteners and value added health food ingredients such as omega-3-fatty acids prebiotics, probiotics etc. Until recently, there has been limited research into functional foods in meats, while functional foods in the dairy sector have been developed to a high degree. But meat is less allergenic than many other foods and much more research into the development and marketing of novel functional meat products is required. Such meat products would open up a new market for the meat industry. Nutraceutical and functional food industry is a very vibrant industry today with the claims and a clear mandate of health, wellness and prevention of diseases as one of its main goals.

Introduction

Hippocrate, an ancient physician, a great thinker and also often referred as father of medicine, for the first time conceive the idea nearly 2,500 years ago that a disease is caused due to natural factors such as environmental factors, diet, and living habits. He advocated the secret of healthy living in healthy food in his famous quote “Let food be thy medicine and medicine be thy food”. But unfortunately, this wisdom about food has faded with the time and we became dependent on synthetic medicines and drugs for even life style disorders. In the last century, research advancement in the field of food science and nutrition established food not only as a source of nutrition but also a source of active food components that aid specific functions and rejuvenated the Hippocrates concept. According to the Department of Health and Human Services, USA, daily diet of a person, plays a crucial role in 5 of 10 diseases which are the leading causes of death, such as diabetes (noninsulin dependent or type 2), stroke, coronary heart disease (CHD), specific type of cancer, and atherosclerosis. In the mid-1980s, term “functional food” was introduce in Japan and defined as food that contain active components either from plant or animal origin and provide physiological benefits in addition to the basic nutritional needs.

In India after rice wheat (Triticum aestivum L.) is the second most important cultivated food crop belonging to family poaceae. Total production of wheat was 760 million tons in India during 2016-2017 (FAOSTAT, 2017) which is second largest in the world after china. In India highest producing state of wheat is Uttar Pradesh followed by Punjab, Haryana, Madhya Pradesh, Rajasthan etc. Wheat is categorize into seven commonly known wheat classes those are hard red spring, hard red winter, soft red winter, hard white, soft white, durum and mixed wheat (Khamis, 2014). 

Abstract

The objectives of the study is to help to create self-employment and provide proper information for different conventional milk based products. Traditional dairy foods have always played a crucial role in preservation of precious milk nutrients and promotion of its use among masses. The demand for milk and milk products is increasing with increasing of world's population. To meet these needs, high-end technology is needed for emerging the capability of milk production and preserving it by producing high quality milk products. The dairy industry is highly localized owing to perishable nature of milk products. Approximately 50% of the total milk produced in India has been projected to be processed into a variety of conventional milk products, such as khoa, chhena, paneer, dahi, ghee, kalakand and a wide range of sweets in rural areas mostly by the rural citizens. Therefore, setting up a milk-based food production system and updating conventional technologies to manage milk-based products on a commercial scale is very important.

1. Sample: One or more units selected from a population of units, or a portion of material selected from a larger quantity of material
2. The Standard Operating Procedures (SOPs) should contain full working instructions as well as information on applicability, expected performance, internal quality control (performance verification) requirements and calculation of results. It should also contain information on any hazards arising from the method, from standards or from reagents.
3. Analytical Samples: The quantity of the analytical sample to be used is usually indicated in tests and assays. Unless otherwise specified, the quantity used may deviate by 10% from that stated. All quantitative determinations should be conducted on triplicate test portions and in these cases, the amount actually taken should be accurately weighed or measured and the result of the analysis calculated on this exact quantity. When substances are to be “accurately weighed” in a test or assay, the weighing is to be performed in such manner as to limit the error to ± 0.1% or less. Quantities smaller than 100 mg should be weighed to the nearest 0.1 mg.

1. Proteins in solution absorb ultraviolet light with absorbance maxima at 280 and 200 nm. Amino acids with aromatic rings are the primary reason for the absorbance peak at 280 nm. Peptide bonds are primarily responsible for the peak at 200 nm.
2. Aromatic amino acids tyrosine and tryptophan give proteins their characteristic ultraviolet (UV) absorption at 280 nm, which is routinely used to estimate protein concentration. Phenylalanine and disulfide bonds also contribute to absorption at 280 nm, albeit slightly.

1. Introduction

Refrigeration may be defined as the process of achieving and maintaining a temperature below that of the surroundings. The aim being to cool some product or space to the required temperature. Refrigerated system or a room system is maintained at a temperature which is lower than the atmospheric temperature. Due to higher temperature of atmosphere, heat enters the system. To keep the temperature of the system constant, heat must be removed continuously from the system. Heat removed must be equal to the heat transferred to the system. One of the most important applications of refrigeration has been the preservation of perishable food products by storing them at low temperatures. Refrigeration systems are also used extensively for providing thermal comfort to human beings by means of air conditioning.

1. Introduction

Cleaning and grading are the first and vital post harvest operations undertaken to remove foreign and undesirable materials such as crop seed, chaff, mud, stones, straws, immature grains, dust, husk and even iron and steel particles from grains as well as fruits and vegetables and to separate the products into various fractions. Generally, the hand threshing and the traditional handling used in most developing countries cause a larger percentage of foreign matter with these crops. Thus, more cleaning is required. Cleaning is essentially a separation process, in which some difference in physical properties of the contaminants and the food units is exploited. 

1. Introduction

Size reduction is applied to all the ways in which particles of the solids are cut or broken into smaller pieces. Size reduction operation of solids is referred to as 'Comminution", some of the common comminutive processes are - crushing, grinding, milling, mincing, and dicing. Breaking down of large sized particles of food substances into smaller particles is a common unit operation in many food processes, e.g. wheat flour, turmeric powder, spices, etc. are mostly used in powdered form. Some ofthe common objectives that are fulfilled by size reduction are:

1. Introduction

To maintain good quality of product & shelf life, it is very important & essential to concentrate on Cleaning & Sanitization process at plant level & to follow the CIP process properly. An attempt is made in the current chapter to present details pertaining to cleaning in fluid milk and milk products manufacturing & processing plants. This chapter will be helpful the students of Food & Dairy Technology for better understanding the process milk plant cleaning & sanitation technology.

1. Introduction

Thermal processing used extensively in the food industry for preservation and shelf life extension of the products (Arnoldi 2001). Heat treatments remain most important in food processing unit operations, not only to achieve desirable changes in eating quality but also for preservative effect by inactivation of microorganisms, destruction of enzymes and insects.Heat is applied in various unit operations through adifferent mode of heat transfer such as conduction, convection, and radiations. 

Introduction

A variety of glassware, plasticware, and other apparatus are used for experimental purposes in a laboratory. They need to be washed, cleaned, dried, and sometimes sterilized before they can be used in the next set of experiments. Glassware needs special care of handling during washing, drying, sterilization, etc. because even a minor mistake in handling of glassware may result in its cracking/breakage and it may become unserviceable. All of these may result in a delay in experimentation if spare piece of the glassware is not available or it may affect serviceability of the associated apparatus.

A large number of glassware such as beaker, flask, measuring cylinder, burette, test tube, etc. are regularly used in a laboratory, which requires careful handling during the different stages of experiment as well as after completion of the experiment while preparing them for another set of experimentation.

Glass being a complex silicate, its properties depends on the type of silicate anions in the structure and the cations formed. Thermal properties of glass changes by the addition of boron oxide (B2 O3 ); hence, borosilicate glass is extensively used in the laboratories. With this brief background information, let us discuss about the necessity, procedures, and precautions during washing, cleaning, drying and sterilization of laboratory-wares. Figure 3.1 shows various advantages of using borosilicate glass in a laboratory.

Good laboratory practices require clean glassware, because an experiment may give erroneous result if dirty glassware is used at any step of the experiment. Hence, the glassware must be physically and chemically clean; and in many cases, it must be microbiologically clean or sterile. The commonly used glassware in a laboratory include test tube, measuring cylinder, pipettes, beakers, flasks, microscopic slides, etc.

Introduction

Measurement of pH of a solution and preparation of different types/strength of buffers are common practices in a laboratory, particularly in the Biochemistry and Molecular Biology laboratories. Therefore, in this chapter you will be acquainted with the procedures of measuring pH of a solution as well as preparation of some of the commonly used buffers in a laboratory.

Potential or Power of Hydrogen Ion (pH)

Measurement of pH of a solution is measuring the molar concentration of hydrogen ion [H+] in the solution, which is an indication of the acidity or basicity of the solution. The symbol pH stands for “power of hydrogen” and numerical value for pH is just the negative logarithm of the molar concentration of hydrogen ions [H+]. The ionic product of water (Kw ) is the basis for the pH scale. The total hydrogen ion concentration from all the sources and it can be experimentally measured.

The pH scale designates the concentration of H+ (and thus of OH−) in an aqueous solution, which ranges between 1.0 M H+ and 1.0 M OH−. The term pH is defined by the expression

Thus, the ionic product of water makes it possible to calculate the concentration of H+, with given concentration of OH−, and vice versa. The value of 7 for pH of a neutral solution is not an arbitrarily chosen figure; it is derived from the absolute value of the ionic product of water. A solution having pH greater than 7 is basic, and the concentration of OH− is greater than that of H+. A solution having a pH less than 7 is acidic.

Introduction

Different types of culture media are used for culturing microbes and plant tissues. Preparing as well as sterilizing them properly following appropriate method with safety for the desired purpose are important parts of research work. In this chapter, we will discuss briefly about different types of media, their composition, preparation and sterilization in laboratory for microbial or plant tissue culture.

Culture Medium

Ingredients mixed to prepare solid, liquid or semi-solid substance to support the growth of microorganism or plant tissue in vitro (outside the body) is called culture medium. Frequently, it becomes important to grow microorganism or plant/animal tissue in vitro for any or all of the following purposes:

• To study characteristic features or properties of microorganisms.

• To identify the causal organism(s) of infection in a clinical sample, so that appropriate remedy can be determined.

• To culture/multiply microorganism or plant/animal tissues in vitro for research or commercial purposes.

• To prepare/produce biological molecules, vaccines, antigens, proteins, metabolites, etc. in vitro for research or industrial purposes.

Introduction

Flowering plants (Angiosperms) are identified by their morphological characteristics like flower, fruit and seeds that help their taxonomic characterization into different species, genera, families and orders. The floral characters which are used for taxonomic characterization of plants include the type of inflorescences, perianth structure, floral symmetry, arrangement of floral leaves, type of androecium, stamens, gynoecium, carpel, ovule, etc. The type of fruit and seed also play important roles in taxonomic classification. In this chapter we will briefly discuss about various morphological features of flowering plants (in botanical terms) that are useful in taxonomy.

Nomenclature of Plant

Nomenclature of plant is the formal and scientific naming of plants. It is related to but distinct from taxonomy for grouping and classifying plants and provide botanical name.

The scientific/botanical nomenclature started with Species Plantarum by Carolus Linnaeus in 1753. Botanical nomenclature is governed by the International Code of Nomenclature for algae, fungi, and plants (ICN), which replaces the International Code of Botanical Nomenclature (ICBN).

ICBN and ICN

International Code of Botanical Nomenclature, (ICBN) was adopted by the VIIth International Botanical Congress held in Stockholm, July1950. While the International Code of Nomenclature for algae, fungi, and plants (ICN) was adopted by the XVIIIth International Botanical Congress held in Melbourne, Australia in July 2011.

Introduction

We come across a variety of chemicals every day in a laboratory. While some of them are relatively safer, others are extremely hazardous. Thus, it is very important to know the potential hazards that can be caused by the chemicals present in the laboratory and the safety measures needed to be followed to avoid the hazards. Additionally, we should also be aware of the steps necessary to be taken on such hazardous situations. According to IUPAC, a hazardous chemical mean the chemical which has the potential to cause damage in various ways. In a laboratory, there are chemicals that might be irritant, carcinogen, respiratory fibrogen, systemic poison, asphyxiant, flammable, etc. which have the potential to cause hazard to the workers or environment. Therefore, it is important to use appropriate procedure to reduce the risk and protect the health/safety of laboratory personnel, the public and the environment.

Types of Hazards Associated with Chemicals

Chemical hazards: The hazards caused by chemicals can be broadly classified into three categories.

1. Health hazard: This is also known as toxic effects of chemical. The risk associated with a chemical in laboratory depends on the extent of exposure and the inherent toxicity of the chemical. Many chemical reactions form the product that might be much more toxic than the reacting chemicals. For example, inadvertent mixing of formaldehyde (a common tissue fixative) and hydrogen chloride results in the generation of bis(chloromethyl) ether, a potent human carcinogen. For most of the chemicals, the route of exposure (through skin, eyes, gastrointestinal tract, or respiratory tract) is more important (Figure 17.1) that must be considered during risk assessment.

Introduction

Proper electrical wiring and earthing are crucial for laboratory safety and functionality. A laboratory houses several installations/equipment that demand stable/continuous power supply. Inappropriate or faulty wiring and earthing may lead to significant risks, including electrical shocks, damage to equipment, and even incidence of fire. Therefore, understanding the requirements of electrical wiring and earthing is essential for designing, construction, and maintenance of laboratory. This chapter aims to provide a basic knowledge of electrical wiring and earthing necessary for a laboratory. Importance of earthing in mitigating electrical hazards and ensuring integrity of electrical installations are also described.

Basics of Electrical Wiring

Basic understanding of electrical wiring is necessary for laboratory safety and efficient electrical functioning. The potential difference between two electrical points (negative and positive) is known as Voltage, which drives the flow of electric current. This is measured as volt (V). The rate of flow of electricity in a circuit is known as Current (I), which is measured as ampere (A). Current can be either direct (DC) or alternating (AC). Opposition/resistance experienced in the flow of current in circuit is known as Resistance (R) and it is measured in ohms (Ω). Higher the resistance means lesser the current flows at a given voltage. The rate at which electrical energy is transferred/utilized in an electric circuit is known as Power (P), which is measured in watt (W). The Power can be calculated as the product of voltage and current (P = V × I).

Introduction

Several hazardous chemicals, microbes, and techniques like recombinant DNA technology are used for research and development work in research Institutes and universities. Therefore, the researchers, technical, helping and supporting staffs as well as the laboratory outputs must be kept free from contaminations. Thus, biosafety is necessary to prevent unintentional exposure/ release of hazardous chemicals, microbes/pathogens, and toxins to the laboratory staff and environment. Following the biosafety guidelines and ethical considerations are essential to maintain safety standards for the researchers as well as the environment. This chapter deals with various biosafety measures and ethical issues to be strictly followed while working in a research laboratory. The required safety level needs to be determined as per the chemical/biological materials are used in the laboratory to keep the researcher/environment safe for sustainable research and developmental activities.

Biosafety

The term biosafety is used to describe the procedure and policies needed to ensure environmental and personal safety. Biosafety refers to the containment principles, technologies and practices that are implemented to prevent unintentional exposure to pathogens, toxins, or their accidental release into the environment. A fundamental objective of any biosafety program is the containment of potentially harmful chemical/biological agents as precautionary safety measures.

Why Biosafety?

With the increasing number of countries adopting molecular biology tools and techniques in life science research and development activities, especially in the areas of agriculture and medicine, the biosafety issues are gaining importance to ensure biological safety for the public and the environment. Conducting research in safe and sustainable manner is not only a personal requirement but essential collective efforts to ensure biological safety for a clean and safe environment. This requires rules, regulations, monitoring bodies, and awareness among the workers as well as the public. Therefore, biosafety per se is an integral part of laboratory research and requires awareness among the researchers so that biological safety can be well taken care from the grassroot level.

Introduction

Probiotics, prebiotics and synbiotics are interrelated terms. Probiotic is discussed in detail in previous chapter. In this chapter, prebiotics and its effect on gut along with probiotics are emphasized. Prebiotics can be defined as non digestible food ingredients that stimulate the growth and/or activity of bacteria in the digestive system in ways claimed to be beneficial to health. They were f irst identified and named by Marcel Roberfroid in 1995. He defined prebiotic as a selectively fermented ingredient that allows specific changes, both in the composition and/or activity in the gastrointestinal microflora that confers benefits upon host well-being and health. Further, FAO defined prebiotic as “a non-viable food component that confers a health benefit on the host associated with modulation of the microbiota.”Whereas the term synbiotics refers to a product that contains both probiotics and prebiotics in the form of synergism. A synbiotic has been defined as “a mixture of prebiotics and probiotics that beneficially affect the host by improving the survival and implantation of live microbial dietary supplements in the gastrointestinal tract, by selectively stimulating the growth and activating the metabolism of one or a limited number of health promoting bacteria, and thus improving host welfare”. As described above, the combined use of prebiotics and probiotics is often described as synbiotic, but the United Nations Food & Agriculture Organization (FAO) recommends that the term “synbiotic” be used only if the net health benefit is synergistic.

Introduction

Let food be thy medicine and medicine be thy food- the age-old quote by Hippocrates, is certainly the tenet of today. Microbial cultures have been used for millenniums in food and alcoholic fermentations and in the past century have undergone scientific scrutiny for their ability to prevent and cure a variety of diseases. Later it became popular in improving human and farm animal nutritive values and recently in aquaculture also. The growing interest in the understanding the role of food in human health has moved from its primary role as a source of energy to the subtle action of biologically active food components on human health. Henceforth functional foods or nutraceuticals are of great demand in present time. The growing scientific evidence suggests that the food supplements containing beneficial bacteria can provide an array of benefits to the host. Now a day’s probiotic fermented foods are in great demand due to the awareness of beneficial aspects of probiotics among people. Several industries focused on the improvement of conventional fermented foods scientifically in order to fetch good market for their products. Particularly, animal feed companies and researchers have been looking for alternative products and strategies that can help to maintain animal gut health in order to prevent or reduce the prevalence of pathogens in the food chain. After understanding the mechanism and beneficial aspects of probiotics in detail at molecular level in earlier chapters, now it is relevant to discuss the applied part of probiotics. In this chapter, commercial importance of probiotics and probiotics derived products at industrial level are emphasized.

Carbohydrates are polyhydroxy derivatives of aldehydes or ketones and undergo all the organic reactions common to the carbonyl groups and hydroxyl groups. The principal milk carbohydrate is lactose. It is a disaccharide. Hydrolysis of lactose with enzyme β-galactosidase yields glucose and galactose.

Lactose is the major carbohydrate present in all types of milk. The first record of isolation of lactose was in 1663, by Bartolettus by evaporation of whey. Milk contains only trace amounts of other sugars, including glucose, fructose, glucosamine, galactosamine, neuraminic acid, and neutral and acidic oligosaccharides.

Lactose plays a significant role in milk and milk products that are

• It plays a major role in the production of fermented dairy products.

• It contributes to the nutritive value of milk and milk products

• It affects the texture of certain concentrated and frozen products;

• It is involved in heat-induced changes in the color and flavor of highly heated milk products.

Status of Lactose

Lactose content varies extensively in the milk of different species. Breed of animal, individuality factors, udder infection, and especially the stage of lactation regulates the lactose content in milk. The lactose concentration in milk decreases gradually and significantly during lactation; this behavior contrasts with the lactational trends for lipids and proteins, which, after a decrease during early lactation, increase significantly during the second half of lactation. Lactose synthesis is hampered during mastitis. Lactose, along with sodium, potassium, and chloride ions, plays the principal role in controlling the osmotic pressure of the mammary system. Thus, any change in lactose content is compensated by a change in the soluble salt constituents. The osmotic relationship between lactose and soluble salts during the secretion of milk partly explains the presence of high lactose in milk with a reduced ash content and vice versa (Table 1).

Being an essential component of the rich Indian past, traditional dairy products and a sophisticated classification of confections have played a significant role in the Indian economy. Depending on how it is processed, it has significant social, cultural, and economic significance. These products have been developed over a long period using a combination of experience, traditional wisdom, and culinary skills. India is emerging as the highest milk- producing country in the world with an annual production of 221.1 million tons in the year 2021–22 (Source: Basic Animal Husbandry Statistics, MoFAHD, DAHD, GoI). An estimated 50% of the milk produced in India is used to make a range of traditional dairy products, including paneer, dahi, ghee, khoa, and shrikhand. The product is not only well- established in the Indian market, but it also has significant export potential due to the large Indian diaspora living all over the world.

All native milk products that have developed over time using locally accessible technologies are referred to as traditional Indian dairy products or Indian indigenous milk products. The Indian masses’ economic, social, religious, and nutritional well- being are greatly influenced by traditional dairy products.

Classification of Traditional Indian Dairy Products

Traditional dairy products in India are typically made, packaged, and/or sold using time- honored methods without the use of machinery or meticulous documentation of the procedure. These products do, however, have several technological advantages, including their widespread appeal, reduced cost of production, straightforward manufacturing process, adoption at the rural level or collection center, large profit margin, and abundance of employment opportunities. These products are categorized according to the manufacturing principle.

Vitamins are naturally occurring, vital nutrients that are needed in small amounts. They are important for bone and tissue health, wound healing, growth and development, immune system function, and other biological processes. These vital organic substances serve a variety of biochemical purposes. Vitamins, like minerals, are not biosynthesized by the body. Consequently, in order to maintain our health, we must obtain them from the food we eat or, in rare circumstances, supplements.

Vitamins in Milk

Together with water- soluble vitamins like vitamin C and B complex and provitamins, milk also contains a variety of fat- soluble vitamins like A, D, E, and K. Some of these vitamins do, however, have very low concentrations.

Vitamin A (Retinol)

Plant foods contain carotenoids, which are the precursors of vitamin A, whereas animal foods provide preformed vitamin A in the form of retinyl esters.

Introduction

Entrepreneurship is crucial for economic development, especially during crises when job losses push people to find alternative livelihoods, often turning to entrepreneurship as a means of survival. Its impact is highly beneficial, promoting growth, wealth creation, and better living standards. In developing countries like India, entrepreneurship offers a path to holistic development, particularly for disadvantaged groups traditionally dependent on agriculture. Uplaonkar and Biradar (2015) noted that entrepreneurship development effectively generates employment, increases income, reduces poverty, improves health, and ensures food and nutritional security. Recognizing the importance of entrepreneurship, stakeholders in the agricultural sector now see its positive impact on business sustainability. Verhees et al. (2011) argued that promoting entrepreneurship in agriculture can significantly reduce poverty and boost rural prosperity, directly addressing socio-economic challenges in rural areas (Bhooshan and Ranjith, 2022). Therefore, supporting the entrepreneurial ecosystem in rural sectors through programs and schemes is essential.