eChapters

Introduction

Over the past few decades, probiotics have become the most promising and emerging field in human nutrition and health across the world including India and other developing countries. The global profit generated from the probiotic market is expected to reach approximately 12,753.4 million US$ by the end of 2026 (Probiotic market report, 2018). The factor fueling the demand for probiotics is the growing awareness among consumers about their health benefits. The association of probiotics with health benefits stretches back to a time before microbes were discovered. However, the scientific evidence of the health benefits of probiotic microorganisms on the wellbeing of the host was firstly proposed by Russian Nobel laureate Ellie Metchnikoff in 1905. He reported that consumption of fermented milk containing Lactobacillus spp. is responsible for the healthy and long life of Bulgarian people (Anukam & Reid, 2007). Afterward, several of scientific progressions were made to reach the current status where probiotics are not only the subject of food and medical research but also the source of a multi-billion dollar global industry.

The term probiotic is derived from the Greek words, which means ‘for life’. According to the Food and Agriculture Association of the United Nations (FAO) and World Health Organization (WHO), probiotics are defined as “live microorganisms which when administered in adequate amount promote the health of the host” (Joint, FAO, 2002). In 2014, International Scientific Association for Probiotics and Prebiotics (IPPA) did minor correction in the definition given by FAO-WHO and defined them as “Live microorganisms that, when administered in adequate amounts, confer a health benefit on the host”

Introduction

Cocoa beans are extracted from the pod of a tree crop Theobroma cacao. It is mainly cultivated in tropical and subtropical countries. In terms of world production, Ivory Coast and Ghana contribute about 65 percent of world production. Cocoa beans are the main ingredient in chocolate. (Teye, E et al 2014). One of the earliest examples of chocolate consumption comes from the Mayans. The Maya drink was prepared with cocoa and water and often included cinnamon and black pepper. It was also known as the “Food of the Gods” and offered to Emperor Moctezuma II by the Aztecs, (Verna, R. 2013). Christopher Columbus was the first European to encounter cocoa in 1502.

Introduction

Color is an important characteristic of food. Based on the color of the food, f irst impressions are made: Is the fruit immature, ripe, or overripe? Is the toast burnt? Is the food fresh? Based on these first impressions, a judgment is made whether the food is safe to eat or not and whether it can be expected to taste good or not. Since color is closely associated with expectations, the addition of color to food is a way to fulfill these expectations. Color is added to food for one or more of the following reasons:

1. To replace color lost during processing,

2. To enhance color already present,

3. To minimize batch-to batch variations, and

4. To color otherwise uncolored food.

The production of fruit and vegetables has recently increased significantly and even surpassed the total grain production in the country due to increased awareness of consumer and positive government initiatives (Schieber et al., 2001; Sagar et al., 2018; Malenica and Bhat, 2020). But due to limited production and area, the importance of excess production during a particular season and waste management arises at highest priority level. Moreover, fruits and vegetables waste are a serious problem for environment as most of themare being disposed of as landfills or directly disposed off to water bodies such as rivers (Wadhwa and Bakshi, 2013). Fruits and vegetables are perishable in nature and rich source of nutrients and vitamins, which results in microbiological contamination (Coman et al., 2019; Bas-Bellver et al., 2020). Foods wastes are usually organic residues generated by the processing of raw agricultural materials into food and are made up of liquid (wastewater) and solids (Bas-Bellver et al., 2020). The wastewater results from the cleaning processes or in the form of excessive or polluted process water. Its dry material content is typically less than 5% by mass. It possibly also contains organic or inorganic cleaning agents or disinfectants. Solid food wastes with an organic origin have remarkably high water content (mostly about 80% by mass). They are usually characterized by a constant quality and purity due to the forgone processes. The fact that these substances are removed from the production process as undesirable ingredients makes them, wastes.

Abstract

Rice is the most produced and consumed source of stable food in J andK, and is excellent in genetic diversity. Rice production is the principal activity and a major source of income for the State. Traditional rice varieties have an enhancing potential in wide range of nutraceutical and functional foods. Besides possessing medicinal and nutritional properties, the by-products generated from these rice varieties in various post harvest operations are equally valuable. Traditional healers and local farmers have been using these traditional varieties in ayurveda and in curing of various kinds of ailments, such as cooling the body in ayurvedic treatments, improving vocal clarity, curing of boils, swellings and skin blemishes. Extracts of brown rice are also used as energy drink in individuals, patients and in treatment of chronic gastric problems, jaundice, dysenteric complaints and to increase lactation and nutrition to Childs. Sticky glutinous rice has been reported in treatment of stomach upsets, nausea, heart-burn and indigestion. Brown rice extracts have been reported in treatments of breast and stomach cancer and warts.

18.1 Introduction

The Packaging Institute International describes packaging as the enclosure of items, packages or products in a wrapped pouch, box, bag, cup, can, tray, tube, bottle or other container for protection, preservation, containment, communication, performance and utility. To an engineer or a scientist, it is technical matter as it is a system designed for efficient delivery of safe food products with high quality, via each and every stage of distribution chain, from production of raw material to food manufacture, packaging, retail, wholesale, consumer, disposal and finally recycling or of resource resurgence. The extent of food packaging is literally broad as it encompasses technological activities such as graphic and machinery design, package manufacture and development, shelf life testing, distribution and marketing. Food packaging science is a discipline which applies principles from four major areas of science (materials science, food science, information science and socioeconomics) to understand the properties of packaging materials, packaging requirements of foods and the packaging system. Both material science and food science have been two major contributors for food packaging development. Material science is important to understand the mechanical strength, barrier properties, appearance and physical and chemical properties of paper, glass, metal, polymer and composite. Food science is important to understand the deterioration kinetics of foods (such as microbial growth, lipid oxidation, moisture loss or gain) governs the food shelf life. Food packaging technology is a science based solutions to address specific food packaging needs; examples are tamper evident packaging, microwavable packaging, modified atmospheric packaging, and controlled atmospheric packaging which are aimed at enhancing safety, quality and convenience for the consumer.

15.1 Introduction

Color of food is the most sensitive part of any commodity as it is the one of the organoleptic characteristic to be noticed and one of the main ways of visually assessing a food before it is consumed. The people associate certain colors with certain flavors and the color of food can influence the perceived flavor in anything from confectionary to beverages. For this reason, food manufacturers add dyes to their products. Sometimes the aim is to simulate a color that is perceived by the consumer as natural, such as adding red coloring to glace cherries, but sometimes it is for effect, like the green ketchup that was launched by Heinz in 2000. If the flavor of a food product is inconsistent with the color, the flavor can often be perceived incorrectly, for example an orange flavored drink colored green could be perceived to taste of lime. In addition, the color of a food substance is important indicator of its freshness. While all of us are aware that food with bright or unnatural colors likely contains food coloring but only few people know that natural foods such as oranges and salmon are sometimes also dyed to mask natural variations in color. The color variation in foods throughout the seasons and the effects of processing and storage often make color addition commercially advantageous to maintain the color expected or preferred by the consumer. Some of the primary reasons include:

The growing concern over the environmental impact of traditional packaging has led to increased interest in biodegradable and edible packaging solutions. These innovative approaches offer sustainable alternatives to conventional materials like plastic, which can persist in the environment for hundreds of years. Biodegradable packaging decomposes naturally, reducing pollution and landfill waste, while edible packaging provides an additional layer of sustainability by allowing consumers to eat the packaging along with the food) These solutions not only address waste management issues but also respond to changing consumer preferences for environmentally friendly products. This chapter explores the importance of sustainable packaging, the different types of biodegradable and edible materials available, and the challenges and future potential of these eco-friendly options.

Introduction

Biofortification is the development of nutrient dense staple food crops using the best conventional breeding practices and modern biotechnology, without sacrificing agronomic performance and important consumer preferred trait (Nestel et al., 2006). It provides a comparatively cost-effective, sustainable, and long- term means of delivering more micronutrients (Saltzman et al. 2014). This approach not only will lower the number of severely malnourished people who require treatment by complementary interventions but also will help them maintain improved nutritional status. Moreover, biofortification provides a feasible means of reaching malnourished rural populations who may have limited access to commercially marketed fortified foods and supplements. Micronutrient malnutrition (MNM) is the major constrain to provide nutritional security to world population of underdeveloped and developing countries. Among the 20 major risk factors of the global burden of disease estimates, Fe deficiency ranks 9th and Zn deficiency ranks 11th, while in the high mortality countries, including India, they rank 6th and 5th, respectively (WHO 2002).

Due to inadequate intakes of vitamins and minerals, half of the world's population suffers from micronutrient malnutrition. An adequate amount of wholesome as well as diverse foods are required for the proper growth and development. In addition to calories requirements, these foods should provide various nutrients. In India, ~70% of children do not get the required calories either due to lack of enough food or diverse food. This indicates that these children are also incapable of fulfilling their protein, minerals and vitamins requirements. Diets that lack diversity can be deficient in certain nutrients. Due to the micronutrient composition of the soil of a region, or because of the intrinsic insufficiency of the normal diet the staple foods of some region can lack particular nutrients. The individual plant foods are often deficient in certain nutrient components due to the varying level and composition of food nutrients in different food crops. Some crops are rich in one component but low in other. For example; legumes are usually high in protein, but deficient in essential amino acids like methionine whereas, wheat and rice are rich in carbohydrates but contain little essential amino acid such as lysine, iron, and lack provitamin A. Relying on a single food crop as major staple source of nutrients thus will not attain a nutritionally complete diet and result in malnutrition and deficiency diseases, which often occur in populations of developing countries, mainly due to poverty. Globally, over one billion people are undernourished and food security is a major issue with the world's population forecast reaching to nine billion by 2050.

Abstract

Bioinformatics is a field of science which studies biology, computer science, and information technology. The ultimate goal is to obtain useful information by data acquisition, analysis and interpretation of sequences data. In the current decade, a huge volume of sequence data is stored in the data bank due to global genome initiatives of many organisms. Management and understanding of the genome data cannot be possible without the bioinformatics tools. A large number of tools and softwares packages are available either commercial or freely online for the bioinformatics applications. The use of bioinformatics tools enhances the research capability for crop improvement through genome sequencing, database management, assembly, alignment, primer designing, markers development, genes prediction and annotation, gene expression, phylogenetics, protein structure identification, metabolic pathways analysis and many other applications.

Introduction

Biological activation of grain or germination is a natural, biological processing techniques and traditional method that can be used to improve the nutritional, functional, and sensory properties of grains. During biological activation of grains, extensive phytochemical changes were reported in a variety of food grains, which reflects a dynamic and complex nutrient flux including remobilization, degradation and accumulation. During this process many hydrolytic enzymes were activated to hydrolyze the starches, proteins and, non-starch polysaccharides, which results in more oligosaccharides and free amino acids. Many nutrients and bioactive compounds reportedly increase and then decrease as they are utilized by the growing plant, which results in degradation of the high molecular weight polymers for generation of bio-functional substances. The biological activation of grains also realizes a significant growth of nutritional value by increasing the bioavailability of nutritional compounds, vitamins, bio-elements and other biologically active substances due to the partial hydrolysis of starch, proteins, hemicelluloses and even celluloses. Biological activation triggers the enzymatic activity in grain and dormant hydrolytic enzymes are activated, breaking down starch, fibers and proteins and leading to an increase in the amount of digestible compounds along with improvement of functional properties without any chemical modification. Also, the activity of some anti-nutritional factors (enzyme inhibitors, haemagglutinins, anti-vitamins etc.) decreases or disappears during activation, allowing a complete valorization of biological compounds in grains. During biological activation hydrolytic amylase enzymes, proteolytic enzymes, and catalase were activated in grains, which enhances the starch degradation, a complex biochemical process and thus lead to the conversion of insoluble granules to soluble starch and dextrin. Apart from positive shifting in the level of nutrition, biochemical activities which occur during activation also generate bioactive components of health-promoting activities through the enzymatic actions that are antioxidants in nature, such as ascorbic acid, tocopherols, tocotrienols and phenolic compounds, thus resulting in an increase of antioxidant activity.

Introduction

Humans have primarily consumed "meat/meat products based on where they lived and how they evolved. According to Food Safety and Standards Act 2006, “meat means fresh meat, poultry and game meat which could be in the form of whole pieces, cuts or comminuted (minced etc.), whereas, meat products are those that have been processed by various methods so that they no longer resemble fresh meat”. These products include meat, poultry, and game products in whole pieces or cuts that are cured salted and dried, processed by non-heat treatment, fermented non-heat treated products, heat treated or frozen products. Edible casings used for sausages etc. also come in the category of meat and meat products. Through a notification dated 12^th September, 2017, the FSSAI has included domestic rabbits (Oryctolagus cuniculus) in the Food Safety and Standards (Food Products Standards and Food Additives) Regulation 2011 in the regulation under meat and meat products.

With the advent of globalisation, there has been an increase in food trade which has led to the introduction of exotic meat and meat products. Moreover, rapidly growing economies like India, now demonstrate a growing appetite for meat. As the consumer demand for high quality meat is increasing in most countries, the meat industry should consistently produce and supply quality meat that is safe and healthy for the consumer to ensure continued consumption of meat products.

I. INTRODUCTION

The fruits and vegetables we eat and flowers and ornamentals we enjoy are biological entities. They breath, produce heat, perspire, are easily damaged, get diseases and finally die. These carry on processes characteristics of all living things before or after harvest to perform the metabolic activities:

1. to maintain cellular organization
2. to transport metabolites around the tissue and
3. to maintain membrane permeability.

1. INTRODUCTION

Packaging waste forms a significant part of solid waste and has caused increasing environmental concerns, resulting in strengthening of various regulations aimed at reducing the amounts generated. A wide range of oil-based polymers is currently used for packaging of various products by different industries including the food industry. These are virtually non-biodegradable and some are difficult to recycle or re-use as they are complex composites having varying levels of contaminants and pose serious health concerns. In the recent years, the use of bio-based and biodegradable products as packaging material has generated a lot of interest amongst researchers as they bring out a significant contribution to sustainable development with a wider range of disposal options.

Bioplastics are polymers that are capable of undergoing decomposition into CO₂, H₂O and inorganic compounds or biomass through predominantly the enzymatic action of microorganisms. Some of these polymers can also be compostable, which leads to their decomposition in a compost site at a rate consistent with known compostable materials. The market for these environmentally friendly materials is expanding rapidly, at the rate of 10–20 per cent per year. The global market for biodegradable polymers exceeds 114 million pounds and is expected to rise at an average annual growth rate of 12.6 per cent to 206 million pounds by 2020.

Bio-based packaging materials are mostly used to pack short shelf-life products, like fresh fruits and vegetables, and long shelf-life products, like pasta and chips, which do not require very high oxygen and/or water barrier properties. However, the inventory of films shows a wide variety in properties, which could make them applicable as a packaging material for other food products with stricter conditions, like MAP packaging. One of the challenges faced by the food packaging industry in its efforts to produce bio-based primary packaging, is to match the durability of the packaging with product shelf-life. The bio-based material offers great potential for the packaging industry. However, it is important to realize that a thorough evaluation of the functional properties of a bio-based material is essential before it can be used as an alternative for the traditional film materials.

14.1 Introduction

Proteins are the substances formed from nitrogen containing amino acids and are principle structural components of body tissues and muscle. In addition, they are used to produce various hormones, enzymes and hemoglobin in human body. Due to the shift from agricultural to electronics and other fast paying industrial development, most of the countries around the world, ceased to be self-sufficient in their food production in particular protein, which is necessary to satisfy their optimum food demands. The biotechnology development and its usage to agriculture have brought hope to them. The need for food by modernization of agriculture has gained much attention throughout the world. However, the necessity for exploring unconventional and non-agricultural means of food production, especially of proteins rich food, cannot be over-emphasized due to the tremendous growth of human populations in several regions of the world. In addition to this, the importance of protein in food nutrient cannot be put aside because its shortage causes various malnutrition problems and the most lethal malnutrition problem is Protein-Energy-Malnutrition (PEM). The United Nations Food and Agriculture Organization estimates that nearly 870 million people of the 7.1 billion people in the world, or one in eight, were suffering from chronic undernourishment in 2010-2012. There are 925 million people undernourished in developed countries (FAO October, 2010). This situation has created a demand for the formulation of innovative and alternative proteinaceous food sources having high nutritional value. These sources have to be non-competitive with food for human consumption, economically feasible and locally available. This demands a search for new protein sources, with high nutritional value, economically feasible and locally available. Use of microbes as a food source is one of the biotechnological innovations that will certainly increase the availability of affordable protein in the world to solve the global food and feed problems.

In recent decades there has been an increased interest in the industrial production of microbiological protein for use in both human and animal nutrition. The main reason behind it is that there has been the shortage of low cost protein sources in view of the increasing human population and living standards, resulting in famine in some parts of the world and increased demand for animal protein in others.

Introduction

Several hazardous chemicals, microbes, and techniques like recombinant DNA technology are used for research and development work in research Institutes and universities. Therefore, the researchers, technical, helping and supporting staffs as well as the laboratory outputs must be kept free from contaminations. Thus, biosafety is necessary to prevent unintentional exposure/ release of hazardous chemicals, microbes/pathogens, and toxins to the laboratory staff and environment. Following the biosafety guidelines and ethical considerations are essential to maintain safety standards for the researchers as well as the environment. This chapter deals with various biosafety measures and ethical issues to be strictly followed while working in a research laboratory. The required safety level needs to be determined as per the chemical/biological materials are used in the laboratory to keep the researcher/environment safe for sustainable research and developmental activities.

Biosafety

The term biosafety is used to describe the procedure and policies needed to ensure environmental and personal safety. Biosafety refers to the containment principles, technologies and practices that are implemented to prevent unintentional exposure to pathogens, toxins, or their accidental release into the environment. A fundamental objective of any biosafety program is the containment of potentially harmful chemical/biological agents as precautionary safety measures.

Why Biosafety?

With the increasing number of countries adopting molecular biology tools and techniques in life science research and development activities, especially in the areas of agriculture and medicine, the biosafety issues are gaining importance to ensure biological safety for the public and the environment. Conducting research in safe and sustainable manner is not only a personal requirement but essential collective efforts to ensure biological safety for a clean and safe environment. This requires rules, regulations, monitoring bodies, and awareness among the workers as well as the public. Therefore, biosafety per se is an integral part of laboratory research and requires awareness among the researchers so that biological safety can be well taken care from the grassroot level.

Abstract

The use of biosensors is growing in the food industry as a means to ensure food safety and quality because of its versatility in determining biological compounds. It is defined as analytical technique used for detection of analyte that combines a biological component with a physico-chemical detector. The most important contribution of biosensors in the food sectors is to control production processes and ensure food quality and safety by reliable, fast and cost effective procedures. Biosensors have been useful for the evaluation of food composition, particularly in functional food products fortified with phytochemicals, vitamins and minerals.. Biosensors are replacing conventional analytical tools since they offer advantages in size, cost, specificity, rapid response, precision and sensitivity. Future progress in the biosensor technology involves the development of nutraceutical release technology within packaging material where it can be used to trigger the controllable release of bioactive components and targeted delivery of protective agents into the food product. The growing consumer demands for safe and quality food has led the food industries to search for quick and reliable methods for which the applications of the biosensor technique in the field of food processing and quality control are found to be promising.

Introduction

The food that we consume should not only contain all essential nutrients like carbohydrate, protein, fat, vitamins, minerals, fibre etc but also should be hygienic and safe. Safety and quality of food is to be ensured not only during food production but also should be continuously monitored till it reaches the consumer (Coles and Frewer, 2013). The characteristics of food are altered during every processing operations viz., cleaning, sorting, transportation, thermal processing, packaging, storage, distribution etc which may be desirable or undesirable. Persistent physical, chemical and microbiological analyses have to be done at all levels of supply chain to ensure the quality of food. Traditional methods of food safety analysis are spectrophotometry, aerobic plate count method, microscopic plate count, titrations, chromatography etc. Though these techniques provide accurate results, the analysis demand cumbersome extraction steps, trained technical staff and are time consuming (Di-Natale et al., 2000). Hence, steady, sensitive and rapid monitoring of food analysis technique is a need of the hour in the present era. Biosensors can effectively replace the conventional techniques owing to its reliability, rapidity, economic and field applicability (Prasad et al., 2014). Biosensors are an attractive diagnostic alternative owing to their in situ detection ability and comparatively reduced detection time from several days to hours, or even minutes (Ruiz et al., 2017). Rapid food analysis can also minimise spoilage, especially in freshly harvested agricultural commodities, such as fruits and vegetables, meat, fish etc. In addition, the portable detection devices provide in-situ detection capabilities without extensive training and assist in integrating real time analysis in food processing centres.

16.1 Introduction

Food safety is most important issue in today's global environment. To safeguard food supply is a very difficult and challenging responsibility, as a consequence of which the world's largest food companies, retailers, and manufacturers have placed very high food safety standards. Although the safety of food has improved exponentially but progress is uneven and food borne outbreaks due to microbial contamination, pesticides, antibiotics chemicals and toxins are common in many countries. International trade statistics (2007) by World Trade Organization (WTO) reported that Europe has accounted for 46% of world exports of agricultural products, where food represents 80% of agricultural exports. The trading of contaminated food among countries increases the risk for outbreaks and consequently, health risks due to microbial pathogens in food are of major concern to all governments.

Biosensors are an important option in the agricultural and food sectors to control production processes and ensure food quality and safety by dependable, fast and cost effective methods. Biosensors have plenty of advantages over conventional analytical tools since they offer advantages in size, cost, inherent specificity, selectivity, rapid response, accuracy and sensitivity. Food microbiologists are always in search of rapid and reliable automated systems for the detection of biological activity. Biosensors provide sensitive, miniaturized systems that can be used to detect unwanted microbial activity or the presence of a biologically active compound, such as glucose or a pesticide. Immunodiagnostics and enzyme biosensors are two of the leading technologies that have had the greatest impact on the food industry. The use of these two systems has reduced the time for detection of pathogens such as Salmonella to 24 hour and has provided detection of biological compounds such as cholesterol or chymotrypsin. The another useful application for biosensors, is detection of genetically modified organisms (GMO), since several countries have laws regulating the commercialization of GMO products and their derivatives. Biosensors are also useful in the implementation of hazard analysis and critical control points (HACCP) plans by verifying process developments and correcting errors in due time. There is joint application of Biosensor technologies and nanotechnologies in many food and agricultural applications such as the development of nano-scale tools for biosafety, nanoscale compounds for food packaging, and nano-sensors for pathogen detection in animals and plants. These are helpful tools in the detection and control of potential food contaminants by the agricultural and food industries.