eChapters

Food and Industrial Microbiology

    1.    Microbiology often has been defined as the study of organisms and agents too small to be seen clearly by the unaided eye—that is, the study of microorganisms

    2.    Two bacteria that are visible without a microscope, Thiomargarita and Epulopiscium.

    3.    Procaryotic cells [Greek pro, before, and karyon, nut or kernel; organism with a primordial nucleus] have a much simpler morphology than eucaryotic cells and lack a true membrane-delimited nucleus. All bacteria are procaryotic.

    4.    Eukaryotic cells [Greek eu, true, and karyon, nut or kernel] have a membrane- enclosed nucleus; they are more complex morphologically and are usually larger than procaryotes. Algae, fungi, protozoa, higher plants, and animals are eucaryotic.

    5.    The ordinary microscope is called a bright-field microscope because it forms a dark image against a brighter background

1. Introduction

The term nanotechnology was first coined by Norio Taniguchi in 1974 to describe controlled thin film deposition (Taniguchi, 1974). Nanotechnology is now generally recognized to include materials with a size in at least one dimension in the 1-100 nm range, although this terminology is not strictly enforced (Rao, 2008). In food science, there are many types of naturally occurring particles that fall within this size range and the use of a strict definition is problematic due to the large distribution of sizes (Kruif, 1998). Many natural foods contain nanoscale components and their properties are determined by their structure. These have been eaten safely for generations. In fact, some of food’s most important raw materials (proteins, starches, and fats) undergo structural changes at the nanometre and micrometre scales during normal food processing(Morris and Parker 2008). Food proteins (for example, native beta-lactoglobulin, which is about 3. 6 nm in length) can undergo denaturation (via pressure, heat, pH, etc. ) 

1. Introduction

Jackfruit is the largest edible tree borne fruit in the world (Baliga et al., 2011). According to Haq (2006) India is considered as the birth place of jackfruit. The major jackfruit producing states in India are Assam, Tripura, Bihar, Uttar Pradesh, the foothills of the Himalayas and South Indian States of Kerala, Tamil Nadu and Karnataka. Jackfruit is a fruit prized in some areas of the world and allowed to go waste in others. According to Sidhu (2012) the extent to which jackfruit is being wasted in India is not exactly documented however, the wastage is approximately 75%. On an average a fully developed fruit may weigh 10-25 kg. Seeds occupy around 10 to 15% of the total weight of the jackfruit (Bobbio et al., 1978). It is reported that jackfruit seeds contain high amount of protein, fiber and carbohydrate (Ocloo et al., 2010). The seeds are viable for not more than three or four days (Sidhu, 2012). According to Abedin et al. (2012) the industrial utilization of jackfruit pulp are varied but the seeds are discarded from those food industries. Moreover, mature fresh seeds cannot remain fresh for long as they tend to germinate easily. So, converting jackfruit seed into flour is an option in order to increase the shelf- life of the seed and can substitute various cereal based flours for developing food products. Dumpling is a type of food which is made out of small pieces of dough wrapped around a filling that may include vegetables, meat, or other ingredients mixed together.

1. Introduction

With increase in consumer awareness and lifestyle the interest for health promoting natural food ingredients has expanded in recent years. However, globally it has become a great challenge to the manufacturers to ensure that such compounds should remain in their active form during processing and storage with an emphasis to its bioavailability profile. Oxidative stress is the major contributor to the pathogenesis of a number of chronic and degenerative diseases including atherosclerosis, Alzheimer’s and Parkinson’s disease, diabetes, DNA damage, carcinogenesis, cardiovascular and neuro degenerative diseases, allergies, premature ageing etc. (Gupta et al. , 2014; Svetli et al. , 2010). Endogenous antioxidant defense mechanisms are not always sufficient to fully scavenge the free radicals formed in the body during various processes. Therefore, dietary components having antioxidant properties, such as tea flavonoids, are very much necessary to supplement the activity of endogenous antioxidants. Tea is one of the most concentrated sources of flavonoids and phenolic acids which show antioxidant activity by sequestering metal ions and by scavenging reactive oxygen and nitrogen species (Frei & Higdon, 2003).

1. Introduction

Kiwi fruit (KF) is very much acceptable for preservation by freeze-drying (FD) technique, since it has very much health benefits such as low calorific value, low glycemic index (GI), more vitamins (particularly vitamin C), maximum load of potassium, presence of variety of pigments, few important enzymes, low allergic response (AR) and finally it is rich in natural antioxidants, polyphenols. It is translucent, green pulp and many black seeds arranged nicely with covered by hairy and brown skin. The KF seeds are small sizes and black colored surround inner circularly through the whole pulp. It gives a nice crunch during consumption and provides a number of essential nutrients. Therefore, it will be remembered that seeds cannot be discarded during processing of KF unlike other fruit (jamun etc. ) seeds.

Pineapple [Ananas comosus (L.) Merr.] is one of the most importanttropical fruit belonging to the family Bromeliaceae and is native to Brazil. It is cultivated in High rainfall and humid coastal regions of peninsular India and Hilly areas of North eastern states. In India, pineapple is commercially cultivated in the states like West Bengal, Assam, Karnataka, Bihar, Tripura, Kerala, Nagaland, Goa, Arunachal Pradesh, Manipur and Meghalaya on large scale whereas on small scale in the states like Gujarat, Andhra Pradesh, Maharashtra, Tamil Nadu, Orissa and Uttar Pradesh etc. It is popularly known as ‘Ananas’ in India and has lot of health benefits due to the presence of a proteolytic enzyme called bromelin, which is used to tenderize meat (Coppensd’ Eeckenbrugge, 2011). It acts as antiamoebic, arbortifacient and also used in correction of stomach disorders (Leal and Coppensd’ Eeckenbrugge, 1996). It is consumed both fresh and processed but mostly as fresh (70%) (Loeillet, 1997). Portuguese are the first people to introduce pineapple to India. The greenhouse cultivation of pineapple was first started in 1500s by Europeans, which led to the varieties like Smooth Cayenne and Queen and transported to all tropical and subtropical regions of the world (Collins, 1951). Due to its ease in transport of its propagules and high tolerance to drought, pineapple cultivation has spread worldwide. But due to its short shelf life, in temperate regions it is consumed more as processed food. The first commercial product made out of pineapple is jams and sweets (Thevet, 1957).

1. Introduction

Underutilized fruits and vegetables are commercially neglected but recognized as rich sources of nutrients and non-nutrient bioactive compounds (Ali et al., 2008). These crops are very popular among local communities in many parts of the world and well accepted in their traditional diets and therapeutic medicines (Animesh et al., 2010). Nowadays, these are gaining significance in research and development programs of many organizations. Meghalaya is endowed with rich and unique diversity of indigenous wild edible fruit species of which most of them are less known and underutilized (Haque et al., 2009). There are rich in functional bioactive components such as vitamins and minerals are the much talked dietary nutrient for women and children health. It is being supplied to body through tablets, vegetables, fruits and other food items (Islam et al., 2012).

1. Introduction

The emergence of food borne illness due to contaminated food is one of the most frequently occurring event and challenge for the food processing industry. This has resulted into economy as well as energy requirement to counter the food borne pathogens. Some of the important pathogenic microorganisms of concern are E. coli, Listeria monocytogenes, salmonella, etc. To address the food safety and quality issues, several non-thermal food processing technologies have been developed in the last decades, with an aim of achieving food safety without significantly affecting the quality of the food product. Cold plasma in one of the non-thermal processing technology, which has shown promising results as evidenced by recent scientific investigations.

The plasma is known as fourth state of matter after solid, liquid and gas state. Plasma contains diversity of energetic species including ions, electrons, atoms, excited molecules and charged particles. In a plasma, a significant number of electrons have such a high energy that no nucleus can hold them. When a gas is subjected to heat or an electric field, some of the atoms become ions and the gas is said to be ionized. An ionized gas in its normal condition, can conduct electric current to a limited extent. If the heat or electric current becomes extreme, many of the atoms become ions. The resulting super ionized gas, which can conduct a large and sustained electric charge. Plasmas are used in several materials processing industries like textiles, electronics, paper and glass industries etc.

Pineapple is a monocarpic perennial herb. Each stem flowers only once and dies after fruiting, similar to bananas, before a side shoot takes over. After the pineapple plant reaches a certain size, natural inflorescence initiation (flowering) occurs in response to decreasing day length and temperature (Bartholomew, 2003). Flowering of Smooth Cayenne pineapples grown under an 8-hour day length was encouraged by a night temperature of 20°C, whereas f lowering was delayed by temperatures of 15 and 20°C, and totally inhibited by 30°C.Flower initiation usually starts in November and lasts through the winter. Flowering in nature is highly irregular, and some plants can fail to produce fruit. Thus, growth regulators such as ethylene (25-50 ppm) and NAA (25 ppm) are used in industrial practise to force plants to bloom. The numerous radish-purple sessile flowers are spirally arranged on the axis, each with a pointed bract. To explain the emergence of developing inflorescence, this stage is also known as the red bud stage. The spike-like inflorescence contains 100 to 200 florets. The petals are white at the base and bluish-purple above the calyx at anthesis. They are ligulae form in shape, about 1.6 cm long and 5 mm wide, and are about 1.6 cm long and 5 mm wide (Collins, 1960).The outer f lower petals are very close together, allowing only small insects to reach the f lower; they have 14 to 20 locules, resulting in a capacity of 6000 to 7000 seeds per 150 floret fruit. After pollination, fruits with 2000 to 3000 seeds are not uncommon (Collins, 1960).

Abstract

The amaranth as a pseudocereal has shown its role in the development and diversification of agricultural products and food which is a priority trend for world agriculture. The amaranth has excellent nutrient profile and technological advantage in various food formulations in comparison to other conventional cereals. Recently, current interest in amaranth also resides in the fact that it has a great amount of genetic diversity, phenotypic plasticity and is extremely adaptable to adverse growing conditions, resists heat and drought, has no major disease problem. This crop is among the easiest of plants to grow in agriculturally marginal lands in hilly area. Moreover, these contain significant amount of bioactive components such as phytosterols, squalene and polyphenols along with good amount of micronutrients such as minerals and vitamins. Amaranth consumption has been shown to improve plasma lipid profile in animals. Methanolic extracts of amaranth have demonstrated anti-hyperlipidemic,anti-diarrheic, anti-fungal and anti-malarial properties. Specific polyphenols such as rutin, isoquercetin and nicotiflorin and phenolic acids and amides with antioxidant activity have been found in amaranth seeds. In addition to above, amaranth, seeds are gluten free and thus they are currently emerging as healthy alternatives to gluten containing grains in gluten free diet for celiac people.

1. Introduction

Sundarbans is claimed to be one of the wonders of the world for its natural resources of halophytic mangrove forest (some 34 species of true mangroves are present), biodiversity and ecosystem that form an extraordinary environment of the globe. Available brackish water environment of Sundarbans provides favourable substratum for algal communities (Naskar and Naskar, 2010). Enteromorpha intestinalis specifically is one of the algal species that is very abundant in the Sundarban region. The production of total seaweeds in India in 2000 was approximately 600,000 tons (wet weight). India produces 110-132 tons of dry agar annually utilizing about 880-1100 tons of dry agarophytes. The seaweed industry in India is mainly a cottage industry and is based only on the natural stock of agar-yielding red seaweeds, such as Gelidiella acerosa and Gracilaria edulis, and algin yielding brown seaweeds species such as Sargassum and Tubineria (Kaladharan and Kaliaperumal, 1999).

Investigational studies indicate that marine algae contain significant amount of nutraceuticals, vitamins, proteins, carbohydrates, fiber and antioxidants. Composition indicates that there is a tremendous scope for food and pharmaceutical applications. These may be used for food fortification but after rigorous studies. Formulation, standarizaiton and characterization should be performed before human use. Studies for exploitation of the bioactive components of the species may open up new avenues. Hence there seems to be a full scope for the realization of outcomes of the research. Further the utilization of the developed processes at higher scales may reap in financial benefits and may prove beneficial to the local people, who are supplier of the raw materials. The present study is an attempt to utilize this India variety of macro-algae for fortification of bakery food products.

Abstract

Demand for starch is increasing day by day and is met by restricted range of crops. In recent years, substantial progress has been made in obtaining starch from non-conventional sources such as sweet potato, buckwheat, sago, water chestnut, which can be used as a substitute for conventional sources. So it is necessary to search for other underutilized and nonconventional sources of starch to meet the increased demand. Typha angustifolia, an aquatic weed containing appreciable amount of starch in its rhizome and roots, can be used for starch isolation to make its use in food and non-food derivatives. In many parts of the world, starch-rich roots of Typha angustifolia are eaten raw or boiled or dried roots are pulverized into a powder, and then, used as a thickener in soups or added to cereal flours to make biscuits and bread.

Successful cultivation of a crop is governed by various factors including the edaphic and climatic conditions of the region, owing to which the crop responds in terms of quality and productivity. Pineapple (Ananas comosus L. Merr.) is one of the major crops cultivated across the tropical and sub tropical countries worldwide. Adaptability of the crop under different climatic conditions, topography, temperature, photo-periodicity, rainfall and edaphic conditions will be discussed in this chapter.

Climatic zone: Pineapple is a tropical fruit crop though it can thrive well in the sub-tropical condition as well.Pineapple grows well inwarm and humid climate ranging from mild coastal climate up to an altitude of about 1000 meters, provided the area is free from frost (Singh, 2014).Pineapple is commercially grown over a wide range of latitudes from 30° N (Assam, India) in the northern hemisphere to 33°S in the Southern hemisphere (South Africa) (Malezieux et al., 2003; Purseglove, 1972).

Topography: Pineapples are easy to cultivatesince they thrive naturally in a variety of places with different elevation, especially in tropical countries (Kaudo, 2014). Though the plant survives at different altitude, the fruit quality and size may alter at different elevation. When pineapple is grown at altitudes greater than 1000 m, smaller fruits with elevated tartness with less attractive pulp colour (Purseglove, 1968).Moreover, fruit flavour can also vary, wherein the fruit tends to bemore acidic and sour when cultivated above 1800 meters, but will be sweet and tasty under 1350 meters (Kaudo, 2014).

Temperature: On an average, the temperatures required for growing pineapples range within 18-30°C (Kaudo, 2014) andmore specifically at 23 - 24 ° C (Neild and Boshell, 1976; Py et al., 1987). The optimum day and night temperatures for pineapple were reported to be about 30°C and 20 °C, respectively (Neild and Boshell, 1976). Bartholomew and Kadzimin (1977), however have reported that leaves and roots grow best at 32°C and 29°C respectively and the growth practically ceases below 20°C and above 36°C. Py and Tisuucau (1965) have observed 25°C to be optimal for fruit ripening.

1. Introduction

Okara is a by-product generated during processing soybean into soymilk or tofu. Okara is rich in fibre and protein. It contains most of the carbohydrates, some of the protein and a small portion of the oil from soybeans. Okara contains approximately 50% fibre, 27% protein, and 10% fat on dry basis (Li et al., 2012). About 1. 2 kg of fresh okara is produced from every kilogram of soybean processed for soymilk (Vishwanathan et al., 2011). Fresh okara from soymilk production has about 80% moisture. The protein present in okara has good nutritional quality and superior protein efficiency ratio. Other soy components that are also likely present in okara include isoflavones (genistein and daidzein), lignans, phytosterols, coumestans, saponins, and phytates (Li et al., 2012). The most common method of utilization of okara is in the form of animal feed. Tofu or soymilk processing plants primarily dump okara as landfill or resort to burning. Okara can be utilized as a fertilizer as it contains various nutrients required for growth of plants. It can be used as a fermentation substrate for production of ethanol. Okara can also be used in pet foods as it contains high levels of extenders and protein. Apart from these, okara can be used in a variety of food products. It can be used in various food products. Utilization of okara is still in the initial stage. It can be used as a replacement of wheat flour in baked products. It can also be used in various soups, salads or vegetable preparations.

Resistant starch refers to the portion of starch and starch products that resist digestion as they pass through the gastrointestinal tract. Resistant starch is the fraction of starch which is not hydrolysed to D-glucose in the small intestine within 120 min of being consumed, but which is fermented in the colon. It can be used as a functional ingredient in various food formulations (Fuentes-Zaragoza et al., 2010). RS has desirable physicochemical properties (Fausto et al., 1997) such as swelling, viscosity increase, gel formation and water-binding capacity, making it useful in a variety of foods. It helps in improving the appearance, texture, and mouthfeel of the final product compared to conventional fibres. Resistant starch can act as a prebiotic for gut microflora (Sajilata et al., 2006). It has hypocholesterolemic effects, is hypoglycaemic and prevents gall stone formation.

Abstract

Quinoa is a pseudo-cereal which belongs to family Chenopodiaceae. Quinoa can act as highly potential crop for areas where ecological conditions do not support other food crops because of its rusticity, tolerance to frost, salinity, drought and ability to grow in marginal soils. Recently, much attention is focused on quinoa for people with celiac disease and allergic to gluten. Quinoa contains high-quality protein, complete with all essential amino acids and is a good source of carbohydrates, lipids, vitamin C, vitamin E (natural antioxidants) and adequate amount of important micronutrients such as minerals. Carbohydrates of quinoa possess excellentfreeze-thaw stability and can be used as thickener in frozen foods.

Quinoa also consists of bioactive components such as phytosterols, squalene, fagopyritols and polyphenols which act as powerful anti-oxidants in-vitro and thus couldprevent degenerative diseases such as coronary heart disease, atherosclerosis and Alzheimer's disease. Fibre accounts for 6% of the grains total weight, and quinoa intake therefore promotes intestinal transit, regulates cholesterol, stimulates the development of beneficial bacterial flora and helps prevent colon cancer. The quinoa grain can be used to combat liver disease, angina and cystitis. The radical scavenging ability and Angiotensin I converting enzyme (ACE) inhibition activity of quinoa is higher than that of amaranth, rice and wheat. Recent studies have identified significant amounts of ecdysteroids, kaempferol, quercetin, two phytoestrogen - daidzein and cenistema in quinoa. Ethanolic extract of quinoa was observed to have Antioxidant and antihypertensive activity.

1. Introduction

Organophosphate (OP) and organocarbamate (OC) pesticides are highly used in modern agriculture due to their low persistence and high insecticidal activity. Although they are relatively less persistent than the organochlorines, they have greater acute toxicity with tolerance level ranging from 1 to 8 μg mL-1 (Mulchandani et al., 2001). The high toxicity of OP and OC neurotoxins and their large use in modern agriculture practices has increased public concern, health risk and the consequent contamination of water and food sources (Buonasera et al., 2010). Development of fast and cost effective analytical tools for onsite monitoring of OP and OC contaminations in food, water and in the environment is highly necessary. An approach toward that has resulted the evolution of acetylcholinesterase (AChE) based biosensors for OP and OC pesticides. Ample literature is available on AChE based biosensors (Zhou et al., 2013). But some intrinsic disadvantages remain that limits its utility and field deployment. The most influencing ones are their inability to sustain in organic solvents and the limited class selectivity that demands alternate bioreceptor for pesticide detection. Given the diversity in mode of action of different classes of pesticide, it is quite challenging to come up with different sensor molecules for each class of pesticides. As a continuous effort towards that goal, attempts have been made to use the Glutathione S –transferses (GSTs; EC 2.5.1. 18) for the purpose. GSTs are a multigenic family of cytosolic proteins with multifunctional biological roles, widely distributed throughout the body and found in the liver, kidney, brain, pancreas, testis, heart, lung, small intestine, skeletal muscles, prostate and spleen (Jancova et al., 2010). The important biological functions those are making GSTs the focus of current research includes its catalytic role in detoxification reaction (Lumjuan et al., 2011) over expressions during phase II metabolism as well as drug resistance associated with anticancer therapies of human and the resistance acquired by certain insects while getting exposure to pesticides (Borah et al., 2016).

Abstract

This review presents an idea on the botanical description, medicinal properties and traditional usage of wild, edible and aromatic plant (Nymphaea alba) in the human nutrition. Nymphaea alba Lin, also known as the White Water lily, White Lotus, or Nenuphar is an aquatic flowering plant of the family Nymphaeaceae. Nymphaea is a weed in India and its production pretty much as good as water chest nut and Nelumbo nucifera. Its rhizome is rich in tannic acid, gallic acid, alkaloids, sterols, flavonoids, glycosides, hydrolysable tannins and high-molecular-weight polyphenolic compounds. It is used as associate aphrodisiac, anodyne, antiscrophulatic, astringent, cardiotonic, demulcent, sedative and antiinflammatory. Further, it conjointly produces calming and sedative effects upon the nervous system, and is beneficial within the treatment of sleep disorder, anxiety and similar disorders. Moreover, the content of starch in the rhizome is said to be 40%. Extensive work has been carried out on agronomic and phenotypic properties for most plants, but relatively little study has been carried out on the properties which are of direct technical and economic interest to competitive food and nonfood industry. Non-conventional food resources and their value addition have attracted attention in the recent yearsfor their potential use as functional ingredients in food formulations, besides food they have also been used for non-food applications. Moreover, Plants provide the predominant ingredients of medicines in most medical traditions. So, there is tremendous potential for the profitable industrial use of these medicinal plants and exploring them for further use. Keywords: Non-conventional food resources, Nymphaea alba, Rhizome.

1. Introduction

The culinary banana (Musa ABB) of Assam and North East India (locally known as kachkal) is an important and cheap source of vegetable in the diet of local people. It is considered as one of the potential sources of carbohydrates, starch, polyphenols, micronutrients and functionally important bioactive compounds (Khawas et al., 2016). Native starches have limited use in food application as it has many weakness such as narrow peak viscosity range, poor process tolerance, low shear stress resistance, hydrophilicity, thermal decomposition, high retro gradation, and synersis and cooked starches will formed a weak, cohesive and rubbery paste. These facts motivated the employment of modified starches as important functional ingredients in processed foods in recent years because of their improved functional properties over unmodified starches (Fleche et al., 1985).

To overcome these problems physical. Chemical and enzymatic modification of Starch is done. Chemical modification of starch generally involves esterification, cross linking, etherification or oxidation of the available hydroxyl groups on the β-D-glucopyranosyl units that make up the starch polymer.

Abstract

Pomegranates are usually consumed as fresh fruit, with only a limited quantity being processed into products like juice, syrup, beverages, wine, confectionary and anardana. The special structure of fruit is one of main factors which limit its industrial processing. Knowing the importance of health benefits of pomegranate and all related aspects, the present study was carried out in order to utilize pomegranate fruit for preparation of pomegranate mouth freshener. Arils of two cultivars like Ganesh and Araktaweredried by two drying methods viz., sun drying (SD) and cabinet drying (CD).Nutritive and palatable mouth freshener was prepared from anardana of two different cultivars as Ganesh (G) and Arakta (A). Production cost of 100g pomegranate mouth freshener is about Rs.55/. It was noticed that moisture content, acidity and ascorbic acid content decreased at the end of storage.