eChapters

1. INTRODUCTION

Fermentation is an age old technology. It has been used by man since antiquity. It has been employed to produce a number of fermented products such as wine, beer, curd, vinegar, pickles and several others. Fermentation was discovered quite early and by chance but the underlying principles were discovered very late. Primarly fermentation was used as a method of shelf-life extension of the produce in the time of glut production, though the final product had modified sensory qualities. Thus, fermentation and drying were the main methods of preservation. With the discovery of new methods of preservation, fermentation as a method of preservation has gone into background and as technique of flavour development and product with modified sensory quality came into practice. In this chapter, fermentation as a method of preservation has been described.

INTRODUCTION

For thousands of years fermentation processes were largely natural phenomena exclusively identified with food preservation throughout the world. Fermentations can play a significant role in meeting the ever growing food requirements of world population providing benefits in the form of innovative food products with improved nutritional value and food quality along side better safety. Fermentations can be used to address a large variety of food related problems and in the development of processes to meet the demand for food. In the wake of estimations made by international organizations that global food demand and population would increase by two-fold by the year 2050, much emphasis has been placed over the ways and means to increase food production and preservation.

Introduction

Wine is a very unique fermented beverage. It was an essential part of various religious and cultural ceremonies in ancient civilizations; and continues to be considered as a ‘drink for elite’. The reason is that wine was considered as ‘Gift of God and ‘God’s nectar’ in religious text books, and medicinal value of the beverage is well supported by modern science. Wine is a beverage well tagged with its geographical origin. This concept originally comes from French concept of terroir; which explains that a varietal wine from a defined region possess distinctive qualities. Wine makers of the past enjoyed this definition of quality for better marketing opportunities. Today, globalization and advancement in wine science created empowered consumers who could clearly discriminate quality of wines. In addition to a product with high sensory qualities, consumers expect a healthful beverage produced in an environmentally sustainable manner. Enology research projects focus on such aspects; and this chapter is a glance on enology advancements and proven health benefits of wine, such as cardiovascular health gut health, dental health etc.

Introduction

Fermented foods are those foods which have been subjected to the action of microorganisms or enzymes that cause desirable biochemical changes and significant modification of the food (Campbell-Piatt, 1994). Fermented foods are usually produced using ingredients of plants or animals in combination with bacteria or fungi. The bacteria or fungi are either sourced from cultures maintained by humans or from the environment. The origins of fermented foods go back many thousands of years, with early evidence of the alcoholic fermentations of barley to beer and grapes to wine. Evidence suggests that fermented foods were consumed 7,000 years ago in Babylon (Battcock and Azam-Ali, 1998).Throughout the world, the fermented foods are used by the people. It plays a major role in the diet in many developing countries. Several traditional fermented foods are prepared in rural areas of many countries.

Fermentation in food processing typically is the conversion of carbohydrates to alcohols and carbon dioxide or organic acids using yeasts, bacteria, or a combination thereof, under anaerobic conditions.

Introduction

Fermentation is an alluring procedure of biochemical adjustment of essential nourishment microorganisms and their enzymes accomplish by lattice. Fermentation is a utilized to upgrade bio-accessibility and bioavailability of supplements from various crops and improves organoleptic properties (e.g., appearance, generation of sour taste and new aroma compounds, and textural changes such as consistency or viscosity) just as increase the shelf life of realistic usability. It makes foodstuff safe not just hindering the growth of pathogenic microorganisms because of antimicrobial growth of lactic acid and it also detoxifies aflatoxin. With these advantages, fermentation is a successful method to reduce the risk of mineral deficiency among population, particularly in developing nations where unrefined cereal or pulses are profoundly consumed. Tragically, it is additionally connected with multiplication of microorganisms, for ex., yeast and molds caused food safety concerns, (Omemu et al; 2011), decrease in provitamin A and antioxidant (Ortiz et al; 2017), carotenoids, just as loss of nutrients and minerals (Hotz & Gibson, 2007).

Fermentation is a minimum cost-efficient procedure for production and preservation of nutrient, therefore fermented food is an important piece of the eating regimen in numerous of the world. In the nineteenth century the scientific study of microbiology began, and just because, the scientific procedure of fermentation was comprehended. several classifications have been utilized to sort the assorted exhibit of the fermented nourishment produced dependent on various microorganisms, and food types, and kinds of fermentation included.

The microorganisms associated with fermentation process fall into three classes: bacteria, yeast, and molds. Yeasts are the principal microorganisms for the fermentation of breads, whereas molds is mainly used in processing of cheese and legumes. Bacteria is included only of the fermentation of cereal product. cereal and tuber fermentation are a lactic and acetic acid producing bacteria. yeast is a piece of mix microbial fermentation by bacteria in weaning food; all the more frequently, be that as it may, alcoholic fermentation of yeast may not totally alluring. Four genera of lactic acid producing bacteria are recognized as overwhelming cereal fermentation to be specific, Lactobacillus, Leuconostoc, Pediococcus, and Lactococcus spp. all of which require carbohydrate for the energy. Lactic acid and other natural acids produced from hexoses bring down the pH from about 6.5 to about 3.6. In the event that the pH is beneath 4.0, fermented product retard the growth of pathogens and along these lines ensure fermented items.

Introduction

Eradication of malnutrition and hunger (Sustainable Development Goal, SDG-2) are the major challenges to humanity. Availability of sufficient nutritionally balanced food is the prerequisite for ensuring global food security. Food security is also critical for achievement of SDG 1 (poverty eradication) and SDG 3 (health and well-being) (UNDP, 2019). Changing climatic conditions accompanied by global warming not only acts a major threat for achievement of SDGs but could actually lead to the reversal of the progress already made. The escalating global temperature has jeopardized the food security throughout the world. A great decrease in the global crop yield has been suspected even with a 2°C rise in temperature. On field, it is often associated with crop nutrient loss, less accumulation of micronutrients and low availability of micro-minerals like iron and zinc. Additionally, the effect of the rainfall and temperature has also been observed. The global food prices are projected to increase by 3–84% by 2050 due to the fluctuations in rainfall and temperature (Hoegh-Guldberg et al., 2018).

Millets are the small seeded grains which belong to family Grassacae/Poaceae. Traditionally, millets are used for the food and feed purpose and are among the oldest cultivated crops. The millet species of commercial importance are proso (Panicum miliaceum), foxtail (Setaria italica), barnyard (Echinochloa esculenta), finger (Eleusine coracana), little (Panicum sumatrense) and pearl (Pennisetum glaucum) in different regions of the world. In India, foxtail millet (priyangava), barnyard millet (aanava) and black finger millet (shyaamaka) have been mentioned even in the Yajurveda texts of 4500 BC (Morya et al., 2017). The records of consumption of foxtail and proso millet are as old as 2000 to 1000 BC in China. Cultivation of foxtail millet in Korean peninsula is documented around 3,500–2,000 BC. Foxtail millet is the oldest of all the cultivated millets and is believed to be originated in Southern Asia. From there, it was spread to Europian nations. In the west, cultivation of millets majorly started after 1850 (ICRISAT, 2019)

Introduction

Fermentation of food dates back to ancient practice of wine making. Fermented foods reflect culture and tradition of folklore and emphasis their close association with nature. Various methods employed for fermenting foods aimed in food preservation to reduce wastage; however lactic acid fermentation enhanced nutrition content and improved organoleptic properties. Experimental evidences on benefits on health and wellness on consumption of fermented foods are being reported. Fermentation process used for preparation of indigenous or traditional foods often depend entirely on natural microflora on the surface of raw material and containers used; Practices of fermenting foods are passed on as traditional know how to generations as a legacy. The fermented food manufacturers rely on commercial starter cultures, and highly reliant on indigenous materials as microbial collections are unique and highly variable according to region and products. Therefore, isolation of novel organisms, development of newer products and interactions are being explored using variety of fermented foods obtained through traditional processes

Introduction

The art of fermentation process is developed as far back as thousand years ago without understanding the role of microorganism in it. It is believed that the process of fermentation originates in Indus valley during 7000 – 8000 BC. Archives of harappan civilization showed that different clay utensils were used for the fermented product and the agriculture and animal husbandry were fully developed during that period. It is evident from the artifacts of Egypt and middle east that the practice of fermentation was common in that part (Farnworth, 2003). The earliest practice of cheese making is originated from Falcate between Tigris and the Euphrates rivers in Iraq, 8000 years back. Later, Egyptians developed the use of yeast in wine and bread making during 4000 BC (Ross et al., 2002). Further, Sumarians prepared beer from fermented barley during 1750 BC. Earlier the fermenting process was an artisan way without any knowledge of microorganism. However, the main turning point was in 1608, when Antony Van Leeuwenhoek first established the use of a microscope and revealed the existence of microorganisms. Another, major finding was Pasteurization by Louis Pasteur in 1861 AD and this was the first report that documented the role of microorganism in fermentation. Later, there was a shift observed in the production of fermented food from local communities to large scale industry. The health effect of fermented food was first reported by Roman historian Pliny, who reported that fermented milk is beneficial for gastrointestinal complains. Further, a French paediatrician proved that Bifidobacteria in food prevents infants against infection (Grill et al., 1995).

1. Introduction

Now a days, foods are not intended to only satisfy hunger and to provide necessary nutrients but also to prevent nutrition-related diseases and improve physical and mental well-being of the consumers (Menrad, 2003). In this regard, functional foods play an important role. The concept of functional foods which has evolved as the role of food in the maintenance of health and well-being and in the prevention of disease, has received increased scientific and commercial interest is recent past. Growth of functional foods markets include increased consumer health awareness, increased popularity of healthy foods, search for food-based drug substitutes, self-medication approach promoted by busy lifestyles and rapidly developing health conscious markets. Functional foods can be defined as foods “that can beneficially affect one or more target functions in the body, beyond adequate nutritional effect, in a way relevant to an improved state of health and wellbeing and/or reduction of risk of disease” (Stanton et al., 2005).

Introduction

Fresh fruit journey from the farm to the consumer’s table is an intricate process which needs a lot of attention. The way that the produce is harvested and handled is of supreme importance in relation to its quality shelf life and general market attractiveness. Improper handling practices during these critical stages can bring both quantitative and qualitative loses hence the perishable produce will lose its commercial value and appeal to the consumers (Kader, 2005). No doubt the significance of successful harvesting and post-harvest handling practices is crucial and has an impact on quality features and safety in case of fresh fruits. Poor management can cause physical assault ripening speed and more susceptibility to the growth of micro-organisms which makes the produce unsuitable for consumption or processing.

Filling converts freshly frozen ice cream into commercially marketable product formats while maintaining accurate portioning, appearance, hygiene, and structural quality. This chapter examines the major filling and forming systems used in industrial ice cream production. It covers cup, cone, moulded stick, extruded novelty, tub, and family-pack filling lines. The chapter explains equipment functions, filling principles, product handling, and operational considerations for different formats. Common filling defects and corrective measures are also discussed. Standard operating procedures for start-up, operation, and shutdown provide practical guidance for plant personnel. Effective filling operations are essential for achieving consistent weight, attractive presentation, packaging integrity, productivity, and efficient manufacturing performance.

A TTO needs a planned budget of five years to sustain it in the long term. This involves budgeting the infrastructure, manpower, research, and outreach programs. The budgetary needs of a TTO will be based on the size and scope of the TTO and the maturity of the parent institution it caters to. The initial years will be focused on capacity development, the appointment of experts, and the establishment of processes of intellectual property management. The mature TTO will generate income through the returns of the licensing agreements and the collaborative efforts with the industry. Nevertheless, external funding through grants and government schemes plays a vital part, particularly in the initial years. The planned budget ensures long-term stability and development of the TTO.
9.1 Budgetary heads
9.1.1 Operational expenditure
Operational expenditure refers to the ongoing costs incurred by a TTO to maintain its day-to-day operations. This includes the cost of maintaining the office, including rent, power and utility bills, computer and network infrastructure, patent management cost, and administration charges. The filing and legal charges also fall within this category. The overheads will be a heavy burden if not planned with finances properly. A TTO has to be provided with a properly planned operational budget that covers the daily operational expenses. The operational expenses help the office perform effectively, sustain the employees, and effectively manage IP. The operational expenses that the budget normally covers include:

Introduction

Fermented meat and fish products have been consumed all over the world since times immemorial and represent one of the most important groups of food. Meat offers its own natural microflora. Hence a wide variety of products have been prepared since ancient times by varying the mixture of meat and salt as well as by the addition of spices and seasonings. Thus, fermented meat products characterize a diversity of flavors and textures and are receiving increased interest from consumers all over the world. Most of these products still depend mainly on local, traditional manufacturing processes since little scientific information is available; but scientific knowledge is very important for consistent production of high quality and safe products.

Meat is an essential part of non-vegetarian diet. It is liked for its unique taste and is a rich source of nutrients like good quality animal proteins, essential amino acids and fatty acids, minerals, trace elements and particularly B-complex vitamins (Singh et al., 2013). The growing concern of consumers regarding health and safety issues has led to the development of functional meat products that promote health and well-being, beyond their nutritional benefits (Grasso, et al., 2014; Tyopponen et al., 2003). This has driven researches in the meat processing sector to increase their efforts in producing such functional meat products, which have incorporated functional ingredients, reduced salt, nitrates/nitrites, cholesterol and fat and modified product fatty acid profiles (Zhang et al., 2010). Fermentation of meat produces a wide range of physical, biochemical and microbial changes in the muscle-based product

Finfish and aquatic invertebrates are part of Fish Genetic Resources (FiGR) that has actual or potential value for culture and capture fisheries. Fish as a group, have the highest species diversity among all the vertebrate taxa. Out of 62,305 species of vertebrates recognized world over, 32,064 (~ 52%) are valid fish species; of which, the great majority (97%) are bony fishes, mainly teleosts; and the remaining (3%) are cartilaginous fishes (sharks & rays) and jawless fishes (lampreys and hagfishes) (Eschmeyer and Fricke, 2012). India is blessed with vast fishery resources, like exclusive economic zone (2.02 m sq. km), coast line (8,129 km), ponds and tanks (2.254 m ha), beels, ox-bow lakes and swamps (1.3 m ha), reservoirs (2.90 m ha), rivers and canals (173,287 km), brackish water (1.235 m ha) (Ayyappan et al., 2011). The country is fortunate to possess vast and varied fish genetic resources in different aquatic ecosystems and being home to 7.84% of global finfish diversity, with 3535 species of fish, of which 3035 are native species, representing 46 orders, 252 families and 1,018 genera. The 500 exotic fishes are also covered and majority of them are of ornamental fishes. Out of the 3035 native fishes, 1016 are freshwater, 113 are brackish water and 1906 are marine species and at least 258 species are of commercially important (NBFGR, 2016-17). India harbours a rich diversity of fishes and four out of 34 biodiversity hotspot areas of the world. The biodiversity hotspots i.e. the Western Ghats and North East India harbours maximum endemic freshwater fishes. The Western Ghats harboring about 306 finfishes out of which nearly 69% are endemic to the region (Gopalakrishnan and Ponniah, 2000). Likewise North East India, another biodiversity hotspot area, harbors about 240 fin fishes and nearly 40% are endemic to the region (Sarkar et al. 2012). In the past one and half decades more than 50 new species were described from these areas and more than 10 new species were described from the marine environment.

7.1 Introduction

Fisheries are emerging as one of the fastest growing food production system all-over the world. The potential for fisheries development world over and especially in India is highly promising in view of physical and biological resources available coupled with technological development. The demand for fish fishery products is ever increasing as the awareness of health benefits are being made available to public at large. The world fish production has not kept pace with demand which means less and less fish is available. Nevertheless, a large fraction of this valuable commodity is wasted due to deterioration after landing or consumer preference. An estimate by FAO [1] indicates nearly 20-25% of the total catches are wasted as postharvest loss. Better utilization of aquatic resources should therefore, aim primarily at reducing this enormous loss by improving preservation of fish and fishery products and upgrading the discarded low value fish to high value fishery products. The processing of fish into different types of products generates considerable waste. Commercially, fish is being processed into fillets, steaks, mince, canned products and a variety of ready-to-eat products. Fish processing plants contributes substantial wastes in the form of skins, bones, offal, exoskeletons from shrimps and nutrients in wash water from surimi plants.

Fish processing waste utilization is an important issue for the seafood industry both from a regulatory standpoint as well as one that has potential economic impacts. It is estimated that only 25-50% of the raw material is utilized for primary products (fillets, canned and stabilized mince production). The remaining 50-75% of the raw material is considered processing waste and is utilized for low-value products or disposed-off at sea. A large volume of solid waste is currently processed into fish meal, fish protein hydrolysates, and fertilizers. A small percentage is processed into higher valued items, such as, seafood flavourants, colourants, chitosan, enzymes, etc.

Introduction Aquaculture is one of the world’s fastest-growing food industries, providing a sustainable source of income for millions of people (Mohanty et al., 2018, Troell et al., 2014; FAO 2018). From the total world’s production around 33% of the all harvested fishes are not only utilized for human utilization for different reasons like size, species, quality, amount, non-accessibility of handling or transportation office. Today, waste generated from fish processing is a major source of pollution in the environment. Even when excellent grade fish is produced, only 50% of it is consumed by humans, with the remainder being discarded as waste. These wastes, which are dumped in an open environment without being treated, produces foul odour, which, as previously said, might be a reason of environmental pollution, water pollution and other municipal difficulties. Fish meal and oil made from low-grade fish and discarded fish parts not only solves many of these health and environmental issues, but also boosts the fishing and fish processing sectors’ overall revenue. With the rapid build-up in yearly extraction since 2000, global usage of natural resources has increased dramatically in recent years, reaching 92.1 billion tonnes and resulting in a 254% rise from 27 billion in the nineteenth century (Coppola et al., 2021). As a result, the research is stepping up its attempts to develop a circular bio-economy that aspires to increase the value of material flows while also achieving sustainable consumption and production. It is a new idea that is gaining traction for the goal of ensuring human quality of life through the effective and sustainable use of resources, energy, and infrastructure.

1. INTRODUCTION

Sensory science is the fundamental physiological and psychological underpinnings of sensory evaluation and includes our basic understanding of the mechanisms of taste and odour, kinesthetic and the various techniques of scaling and statistical analysis, whereas, sensory evaluation could unfold the application of this knowledge and the development of specific methods for the evaluation of the discriminative, descriptive and affective nature of products, in a problem-solving situation. When the Quantitative Descriptive Analysis (QDA) methodology was first described, it represented a decade of experimentation and study of the issue that were perceived at the time to be the impediments in solving “the problem of flavour characterization” Further, new developments and knowledge about sensory evaluation were beginning to have a positive effect on its acceptance by the food industry, but much more needed in be done if it is to contribute in a meaningful way. Today, it is indisputable that flavour is an important attribute affecting consumer acceptance of food and it is taste plus odour. The perception of taste is merely the detection of non-volatile tastants by the tongue while in other words, the perception of odour is the detection of odoriferous volatile aroma compounds by the olfactory nerves ending in the nose. The combination of all the possible flavour stimuli that could be encountered is as vast as the galaxy.

Introduction

How one can differentiates between sensory science and sensory evaluation? Perhaps the simplest way would be to think of sensory science as the fundamental physiological and psychological underpinnings of sensory evaluation. It could include our basic understandings of the mechanisms of taste and odour, kinesthetic and the various techniques of scaling and statistical analysis, whereas, sensory evaluation could designate the application of this knowledge and the development of specific methods for the evaluation of the discriminative, descriptive and affective nature of products, in a problem-solving situation. When the quantitative descriptive analysis (QDA) methodology was f irst described, it represented a decade of experimentation and study of the issue that were perceived at the time to be impediments to solving “the problem of flavour characterization” (Stone et al., 1974). As noted by Pangborn (1964), new developments and knowledge about sensory evaluation were beginning to have a positive effect on its acceptance by industry, but much more needed to be done if it was to contribute in a meaningful way. Today, it is indisputable that flavour is an important attribute affecting consumer acceptance of food. “Flavour is taste plus odour”. The perception of taste is merely the detection of non-volative tastants by the tongue. On the other word, the perception of odour is the detection of odoriferous volatile aroma compounds by the olfactory nerves ending in the nose. The combination of all possible flavour stimuli we could encounter is as vast as the galaxy. However, most of the attempts to define these experiences have historically been over simplified.