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Agriculture has been the base of human livelihood since ancient time when the man started planting of seeds of some edible plants now called Agricultural crops. Domestication of food plants and animals was started with the development of human civilization. Thus agriculture can be explained as science and technology of growing food crops and rearing of animals. Still in several countries of the world, most of population is based on agriculture for their livelihood particularly in rural areas of that country. According to Williams and Haq (2002) there are over 7000 species of plants that can be used for food. The edible plants species grown for food are called agricultural crops. The crops grown in garden are known as garden cops or Horticulture crops. Horticulture is a branch of agriculture, which deals with intensive cultivation of garden crops such as fruits, vegetables, f lowers, ornamental plants, plantation crops, spices, medicinal and aromatic plants, and mushroom. It also deals with post-harvest technology of horticultural crops. Processing and Preservation of fruits and vegetables is a part of Post-harvest Technology of horticultural crops which deals with methods of processing and preservation of these crops to extend the shelf life of fruits and vegetables after harvesting.

All horticultural crops have their own importance in our society. Fruits, vegetables and spices are major ingredients of our meal. Spices add taste and flavor to our food and also have high medicinal values. Flowers and ornamental plants are used for decoration of environment and landscape. Aromatic and plantation crops have become industrial crops. Mushroom growing is a great enterprise. Among these crops fruits and vegetables are major crops and have been considered presently as highly economical and important food crops. There are 1200 crop species are used for vegetable purpose in the world (Hazra and Som, 2006). Fruits and vegetables are richest and cheapest source of all essential nutrients (carbohydrates, fat, protein, vitamins and minerals) required by our body for proper functioning of body organs and maintaining good health. Besides these nutrients they also supply water and fibers to our body which play vital roles in our body. Fruits and vegetables have important place in our daily diet and based on survey made by experts FAO WHO (2003) has recommended 400 g/capita/day of fruit and vegetables (fruits -100 g/capita/day and vegetables -300 g/capita/day) to be consumed for good health and prevent chronic diseases. Therefore, the demand of these food commodities has increased in the modern society due to increased awareness of the nutritional importance of fruits and vegetables. So that people must be aware that what part of fruit and vegetable is to be consumed?

Fermentation process is known to humans since ancient times. It involves the transformation of raw materials into value added or nutritional products as a result of microbial action or their growth. The fermentation as a science involves aspects of the biological action of microbes, biochemical changes in the substrates and metabolites formed and their effect on food, health and nutritional applications. While fermentation as technology, involves chemical engineering, food engineering and instrumentation aspects and commercialization of the products prepared keeping in view the consumer acceptability. The term fermentation is derived from the Latin word ‘Fervere - to boil’ thus describing the actions of yeasts on extracts of fruits or malted grain. Fermented foods and beverages enhance the pleasure of eating and their nutritional roles include indirect contributions through subjective enhancement of appetite. The instant mixes for fermented or fermentable products such as idli, dosai, dhokla and bhatura mix can reduce the overnight fermenting of the raw materials required for preparation of these products. The instant mixes for idli & dhokla are quite common in the market. However, instant mix for Jalebi and Bhatura are still emerging. Such products add convenience to the consumers and products can be prepared instantly. Certain other fermented foods include dairy products, pickles, sauerkrauts, Kanji, beverages, food supplements and nutraceuticals from fermented biomass.

In 1907, the Russian Nobel laureate, Dr. Elie Metchnikoff, fascinated by the unusual longevity of Bulgarian farmers, consuming beneficial lactic acid bacteria by way of traditional fermented milk products, suggested that regular intake of friendly microbes could benefit human health. Although Dr. Metchnikoff ’s recommended bacteria for the purpose proved largely ineffective, the theory was carried forward with moderate success until the war on bacteria began because of the advent of sulfa drugs and antibiotics (Scott, 2012). However soon after their rampant use and the realization of the rapid spread of antimicrobial resistance, researchers turned yet again to ecological notions and the time was fertile for the advent of probiotics. By the 1980’s the term had stabilized both in medical and veterinary literature. The mid 1990’s witnessed a surge of exponential studies on probiotics, the definition of Probiotics emerged in 2001 and the years thereafter saw tremendous progress in the science of probiotics.

Almost simultaneous was the study on the study of the intestinal microbiota when Alfred Nissile demonstrated that transferring members of the human gut microbiota to healthy typhoid carriers helped in Salmonella being cleansed from their system (Nissle, 1916). It was then, that the importance of the gut microbes in prevention of infection became evident. What followed was the advent of culture independent, modern biotechnological tools for whole genome sequencing and the successful launch of two most ambitious projects - Human Microbiome project (Turnbaugh et al., 2007) and the Met Hit project (Metahit, 2009; Qin et al., 2010). They provided fascinating insights into the highly complex microbial ecosystem harboured by the human body. The term Microbiome (refers to the collective genome of our microbial inhabitants) had entered literature in 2001 and the concept of gut microbiota in maintaining overall health gained wider attention.

Introduction

Since the last century, nutritional science has made a remarkable contribution to the biological sciences through identification of diverse nutrients and their sources, functions, requirement, deficiency symptoms and their remedial measures etc. The advent of high-throughput sequencing technologies together with bioinformatic tools identified the presence of 100 trillion of microorganism belonging to all forms of life in the gastrointestinal tract. In fact, the whole gut microbial consortia are considered as a virtual organ in human and animals and is responsible for the development and functioning of series of essential physiological activities including digestion, absorption, metabolism, immunity, homeostasis, brain and gut development, resistance against disease etc. According to the modern scientific dogma, the human being is in fact a “Superorganism”, carrying consortium of numerous symbiotic Eukarya, Bacteria, Archaea and Viruses. The gut microflora has been co-evolved with human and presumed to maintain an intricate balance among different microbial communities. Upon introspection about the functionality of the gut microflora, it is amply clear that a few of these microorganisms have the ability to make healthy state; while other members are responsible for health-related problems including food poisoning, diarrhoea, abdominal pain, indigestion, inflammatory bowel disease, predisposition to colon cancer, compromising immunity, allergy etc.

Preamble

Historically, food has been an integral part of human civilization and diet with varied food components has sustained human life on this planet over the centuries. Similarly, culturing of food with natural fermentation as means of preserving foods with extended shelf life for future use. In the background of sustaining food security, advances made in biotechnology and molecular biology has provided ample scientific platform to use the advances in increasing and expanding food production on a global magnitude, so as to meet the needs of an ever-growing world population.

The intestinal microbiota is a key component of both the metabolism and immunity of humans and animals. The desirable microbes do have a significant role to play in healthcare through control of digestive disorders and foodborne illnesses. The continued research in this area over the years has led to the identification of important molecules that regulate major functional attributes in the betterment of human health and wellness in the long run. The science and technique of genetic engineering through molecular biology approaches has enabled in enriching the beneficial molecules for the benefit of human life. The accuracy in targeting and designing of molecules will go a long way in developing genetically modified (GM) lactic acid bacteria (LAB) and GM probiotics to significantly rewrite the medical care with food-based approach in the form of targeted specific diets – “Dietomics”.

Introduction

Off late our understanding of the composition and functions of the human gut microbiota has increased exponentially and there is a great interest in producing healthy foods in particular that which have an impact in improving the gastrointestinal health such as probiotics, prebiotics and synbiotics. Many of these functional foods are dairy based and have been accepted greatly worldwide. Nonertheless, there has been a need for the development of nondairy probiotic, prebiotic and synbiotic products (Salmeron 2017). This has prompted food scientists and food industrialists to study the feasibility of applying other fermenting substrates such as cereals for the development of innovative nondairy fermented functional foods. The importance of including nanoscience and nanotechnology techniques for the creation of fermented cereal beverages that contain specific bioactive nanoparticles and their role in food packaging systems will be discussed.

Introduction

The entire living organism from the smaller creatures viruses to the advanced human beings are composed of a set up of biochemical reactions which form the metabolism. These set of metabolic reactions are responsible for the life of organism. The rate of these reactions is a major concern for effective working of organism which is governed by biocatalyst. These biocatalysts are known as enzymes, which accelerates rate of biochemical reaction. These are not utilized in reaction. In 1877, Wilhelm Friedrich Kuhne (Kuhne, 1877) proposed the term enzyme which means ‘in yeast’ and is derived from the Greek word; inzume “leavened” or “in yeast”. At the molecular level of study it is observed that enzymes are proteins which are functional as tertiary and quaratanary structures. Enzymes have active site for the binding to substrate. The active site has two positions; binding and catalytic site (Ogeston, 1948). The former help to bind the substrate with the enzyme followed by the catalysis of reaction by the catalytic site.

Introduction

Since long time fermentation process is in use for the production of functional food products for human welfare. SSF is a cost effective industrially important method that could be used by food industries to improve the nutritional profile of natural substrates (Salar and Purewal, 2016; Postemsky et al., 2017). The process is being carried out by trained microbiologists in the presence of aseptic conditions using suitable starter culture on steam sterilized substrates. Steam sterilized substrates possess minimal amount of moisture so as to support the microbial growth during the fermentation period. Growth of microbial strains on specific substrates during the fermentation time results in formation of koji (antioxidant rich fermented product). Selection of substrates and starter microbial strains is an important step in the fermentation process which will determine the nutritional properties of fermented product. One of the major advantages of SSF at industrial level is its potential to be utilized on cereal grains, fruits, vegetables and even agricultural wastes (Salar et al., 2012; Postemsky and Curvetto, 2015; Bhanja et al., 2009). Most common substrates that are being used as solid platform for the growth of microbial strains are cereal grains.

Introduction

Wine is a very unique fermented beverage. It was an essential part of various religious and cultural ceremonies in ancient civilizations; and continues to be considered as a ‘drink for elite’. The reason is that wine was considered as ‘Gift of God and ‘God’s nectar’ in religious text books, and medicinal value of the beverage is well supported by modern science. Wine is a beverage well tagged with its geographical origin. This concept originally comes from French concept of terroir; which explains that a varietal wine from a defined region possess distinctive qualities. Wine makers of the past enjoyed this definition of quality for better marketing opportunities. Today, globalization and advancement in wine science created empowered consumers who could clearly discriminate quality of wines. In addition to a product with high sensory qualities, consumers expect a healthful beverage produced in an environmentally sustainable manner. Enology research projects focus on such aspects; and this chapter is a glance on enology advancements and proven health benefits of wine, such as cardiovascular health gut health, dental health etc.

Introduction

Fermented foods and beverages comprise an indispensable part of the human diet. They can be generally defined as those foods and beverages produced via controlled microbial growth and conversion of various substrates using either enzymes or as whole cell itself, as shown in Figure 1.

Introduction

The improvement of the quality of foods can be made by fortification of foods nutrients quality and quantity in food. It can be a very cost effective public health intervention. As a result of the high consumption pattern of dairy products viz. yogurt, the fortification process will effectively diminish or prevent diseases associated with nutritional deficiencies (Gahruie et al., 2015). However, due to nutrients deficiencies in human population especially in certain stages of life, holding, importing, and consumption of fortified foods is increasing (Preedy et al., 2013). As a general rule, addition of one or more essential nutrients to a food and increasing their concentration in that particular food to levels higher than normal is known as fortification. This is intended at preventing and correcting shortages in one or more micronutrients in the society or specific population groups (Bonner et al., 1999). The major ways of food fortification are (1) commercial and industrial fortification for wheat flour, corn meal, cooking oils, (2) bio-fortification of crops to augment their nutritional value, which can include conventional as well as selective breeding, and genetic engineering and (3) house hold fortification like vitamin D drops. Fermentation has twofold outcome on glycemic index though there are conflicting results after consumption. The reduced glycemic index of fermented food has been featured to the production of short?chain organic acids during fermentation.

Introduction

Fermented meat and fish products have been consumed all over the world since times immemorial and represent one of the most important groups of food. Meat offers its own natural microflora. Hence a wide variety of products have been prepared since ancient times by varying the mixture of meat and salt as well as by the addition of spices and seasonings. Thus, fermented meat products characterize a diversity of flavors and textures and are receiving increased interest from consumers all over the world. Most of these products still depend mainly on local, traditional manufacturing processes since little scientific information is available; but scientific knowledge is very important for consistent production of high quality and safe products.

Meat is an essential part of non-vegetarian diet. It is liked for its unique taste and is a rich source of nutrients like good quality animal proteins, essential amino acids and fatty acids, minerals, trace elements and particularly B-complex vitamins (Singh et al., 2013). The growing concern of consumers regarding health and safety issues has led to the development of functional meat products that promote health and well-being, beyond their nutritional benefits (Grasso, et al., 2014; Tyopponen et al., 2003). This has driven researches in the meat processing sector to increase their efforts in producing such functional meat products, which have incorporated functional ingredients, reduced salt, nitrates/nitrites, cholesterol and fat and modified product fatty acid profiles (Zhang et al., 2010). Fermentation of meat produces a wide range of physical, biochemical and microbial changes in the muscle-based product

Introduction

Food is the most vital prerequisite for sustenance of human life and provides adequate nutrients for the metabolism. Recently several hypothesis supports that, beyond meeting nutritional needs, they play beneficial roles in improving health, and minimize the risk of illnesses (Roberfroid, 2000a). In this way, a novel term “functional food” was introduced and plays an outstanding role in improving the overall conditions of the human body (e.g. pre- and probiotics), lessening the threat of some ailments (e.g. cholesterol-lowering products), and also as therapeutics for several diseases (Roberfroid, 2000a, Roberfroid, 2000b, Mollet and Rowland, 2002).

Recently throughout the world, an extensive array of functional foods were developed which includes prebiotics, probiotics, synbiotics, foods supplemented with anthocyanins, phytosterols, isoflavones, antioxidants, and fat, sugar and salt-reduced foods. Amongst them, probiotic functional food has exerted beneficial effects on the health and is comprised of both probiotic dairy foods and probiotic non-dairy foods. The demand for dairy probiotic foods are increasing as it attributes to the beneficial health effects on the host by improving the survival of gastrointestinal microflora, production of bioactive compounds and prevention of lactose intolerance. Consequently, production of these foods is a significant research importance for both food manufacturing units and research institutes.

Probiotics are the important ingredients for foods with a “healthy” image. It could be combined with other beneficial ingredients or simply used to supplement the natural functional qualities of whole foods. The amalgamation of probiotic bacteria with nutrient rich foods, like dairy products, will have the additional value of improving consumer nutrition.

Introduction

Over the past few decades, probiotics have become the most promising and emerging field in human nutrition and health across the world including India and other developing countries. The global profit generated from the probiotic market is expected to reach approximately 12,753.4 million US$ by the end of 2026 (Probiotic market report, 2018). The factor fueling the demand for probiotics is the growing awareness among consumers about their health benefits. The association of probiotics with health benefits stretches back to a time before microbes were discovered. However, the scientific evidence of the health benefits of probiotic microorganisms on the wellbeing of the host was firstly proposed by Russian Nobel laureate Ellie Metchnikoff in 1905. He reported that consumption of fermented milk containing Lactobacillus spp. is responsible for the healthy and long life of Bulgarian people (Anukam & Reid, 2007). Afterward, several of scientific progressions were made to reach the current status where probiotics are not only the subject of food and medical research but also the source of a multi-billion dollar global industry.

The term probiotic is derived from the Greek words, which means ‘for life’. According to the Food and Agriculture Association of the United Nations (FAO) and World Health Organization (WHO), probiotics are defined as “live microorganisms which when administered in adequate amount promote the health of the host” (Joint, FAO, 2002). In 2014, International Scientific Association for Probiotics and Prebiotics (IPPA) did minor correction in the definition given by FAO-WHO and defined them as “Live microorganisms that, when administered in adequate amounts, confer a health benefit on the host”

Introduction

One of the main properties of fermented foods whether alkaline or acidic is the presence and activity of beneficial organisms which themselves make these foods functional. These functional micro organisms have varied roles like conversion of the native constituents of raw materials to forms in which they are more bioavailable, formation of taste, colour and flavouring compounds thereby imparting pleasantness to foods, removal of anti-nutritive factors in food by converting them into much less harmful compounds or degrade certain native toxic elements present in food, formation of biopreservatives which will extend the shelf life of foods and provide microbial security, as pathogens and spoilage microorganisms fail to readily grow in such hostile conditions. These functional microorganisms also produce in foods certain health-promoting bioactive compounds like enzymes, vitamins, minerals or increase the digestibility which may not have been in the original food or raw material before fermentation. In other words they improve the nutrition profile of the foods or naturally fortify foods. Some of these beneficial effects may also lead to the formation of certain compounds which show antioxidant properties in foods. These very roles played by these functional micro-organisms form criteria in their selection for use in food fermentations when they are intentionally added to foods both to initiate and accomplish desired fermentations.

Introduction

Foods prepared by fermenting raw ingredients of plant or animal sources naturally or mixing it with the starter culture (s) containing functional micro flora is called fermented foods. The functional micro flora used in starter culture is responsible for biochemical and organoleptic alteration of the substrates into edible yields which are socially and culturally appropriate to consumers (Tamang et al., 2010a, Tamang et al., 2010b). Fermented food has its own characteristic microflora and this group of micro flora increases the nutritional value by increasing the levels of essential amino acids, vitamins, proteins, etc.

Fermented foods play a major role in meeting nutritional requirement and socio economic development of developing countries. They are important components of the diet as staples, adjuncts to staples, condiments and beverages. A wide variety of fermenting substrates are available such as cereals, pulses, soya beans, flowers, milk, fish, meat, etc. A wide majority of plants and their parts are often utilized in the preparation of starter culture (Deori et al., 2007, Deka and Sarma, 2010).

Introduction

Eradication of malnutrition and hunger (Sustainable Development Goal, SDG-2) are the major challenges to humanity. Availability of sufficient nutritionally balanced food is the prerequisite for ensuring global food security. Food security is also critical for achievement of SDG 1 (poverty eradication) and SDG 3 (health and well-being) (UNDP, 2019). Changing climatic conditions accompanied by global warming not only acts a major threat for achievement of SDGs but could actually lead to the reversal of the progress already made. The escalating global temperature has jeopardized the food security throughout the world. A great decrease in the global crop yield has been suspected even with a 2°C rise in temperature. On field, it is often associated with crop nutrient loss, less accumulation of micronutrients and low availability of micro-minerals like iron and zinc. Additionally, the effect of the rainfall and temperature has also been observed. The global food prices are projected to increase by 3–84% by 2050 due to the fluctuations in rainfall and temperature (Hoegh-Guldberg et al., 2018).

Millets are the small seeded grains which belong to family Grassacae/Poaceae. Traditionally, millets are used for the food and feed purpose and are among the oldest cultivated crops. The millet species of commercial importance are proso (Panicum miliaceum), foxtail (Setaria italica), barnyard (Echinochloa esculenta), finger (Eleusine coracana), little (Panicum sumatrense) and pearl (Pennisetum glaucum) in different regions of the world. In India, foxtail millet (priyangava), barnyard millet (aanava) and black finger millet (shyaamaka) have been mentioned even in the Yajurveda texts of 4500 BC (Morya et al., 2017). The records of consumption of foxtail and proso millet are as old as 2000 to 1000 BC in China. Cultivation of foxtail millet in Korean peninsula is documented around 3,500–2,000 BC. Foxtail millet is the oldest of all the cultivated millets and is believed to be originated in Southern Asia. From there, it was spread to Europian nations. In the west, cultivation of millets majorly started after 1850 (ICRISAT, 2019)

Introduction

Cereals are grown over 73% of the total world harvested area and contribute over 60% to the world food production providing dietary fibre, proteins, energy, minerals and vitamins required for human health. Cereals like rice, maize, wheat, barley, sorghum, and oats are chiefly used in the preparation of traditional fermented foods and beverages. However, the nutritional quality of cereals and the sensory properties of their products are sometimes inferior in comparison with dairy products. The reasons behind this the lower protein content, deficiency of certain essential amino acids, the presence of determined anti-nutrients (phytic acid, tanins and polyphenols) and the coarse nature.

Introduction

Fermentation of food dates back to ancient practice of wine making. Fermented foods reflect culture and tradition of folklore and emphasis their close association with nature. Various methods employed for fermenting foods aimed in food preservation to reduce wastage; however lactic acid fermentation enhanced nutrition content and improved organoleptic properties. Experimental evidences on benefits on health and wellness on consumption of fermented foods are being reported. Fermentation process used for preparation of indigenous or traditional foods often depend entirely on natural microflora on the surface of raw material and containers used; Practices of fermenting foods are passed on as traditional know how to generations as a legacy. The fermented food manufacturers rely on commercial starter cultures, and highly reliant on indigenous materials as microbial collections are unique and highly variable according to region and products. Therefore, isolation of novel organisms, development of newer products and interactions are being explored using variety of fermented foods obtained through traditional processes

Introduction

Fermentation has been an integral part of food processing throughout the history of mankind. In beginning, it was mainly preservation method, then it became key process in substantial sensory characteristic development and now it is utilized to improve overall properties of outcome from major industries like bakery, dairy, beverages, etc. The human understanding of controlled fermentation process changes its fundamental objective by exploring its success in development of rheological and texture characteristics.

The biochemical transformation of raw food due to fermentation affects the organoleptic as well as rheological properties of a product. The changes in rheological as well as sensory properties is related with the modification of major component by the action of microorganism. For example, transformation of polysaccharides in bread dough by action of yeast produce ethanol, CO₂ and other simple compound which modify sensory as well as rheology of dough. The major micro-structural, bio-compositional and bio-chemical changes are due to microbial action are frequently described to understand the effect of fermentation on rheological as well as sensory properties of food products. The rate and quality of these associated major changes mainly depends upon concentration of saccharides for growth of microorganism as well as nature and existing microflorae. Hence, a proper monitoring of changes in rheological and sensory properties is necessary to optimize the controlled fermentation as well as design of process parameters from engineering and consumer point of view.

The biochemical changes directly responsible for changes in basic rheological and sensory characteristic including flow behavior, consistency, viscoelastic properties, hardness, adhesiveness, color, aroma, flavor and mouthfeel. The food industry is so diverse and exploring new and possibilities to catch diverse consumer worldwide, a proper understanding of effect of fermentation on mentioned properties will be key to hold competitive market. Hence, the present chapter will cover the basics of rheology and its role in food industry, effect of fermentation on rheological and sensory attributes of major class of food.